Rw5G021010
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000469
Physical Location & Seq
Forward (+)
27929746 .. 27930645
900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021010.1

Sequence Viewer

Length: 747 bp
ATGGAGGCTTCTGGAGATGAGGAGGAGATAGACCAACTTATATGTGTAACTGGTCGTCTTATTCAACTCTATCTTGAAAAGTACATTCAGAAAACTCCATATATGACAAGCTCCCAAACAGGTTATATTTGGTTAATGGAAGTGTTAGAGGGGAATGAAAGTAGGTGTTATAACCAGTTTAGGATGGATAAAAACTGCTTCTTAATGCTATTGAATGATGTTGAGAAGATGTTGAAGGGTTCAAAAAACATAAGTACCGTTGAAATGTTAGCTATGTCTCTATATATTTTGGGACAAGGTCAAACCAATAGGAATACACAAGAGCGTTTTCAACGTTCTGGTGAGACGGTTAGTAGATATTTTGATAAAATGCTCAAGATTTTCTATAATATGGCGAAAGTGCTCATAAAGCCAGAAGATCCCGAATTTACAAGCATCCCAAATGAAATTCTAAATGACTCAAGATACATGCCTCATTTTAAGGATTGTATTGGCGCTATTGATGGTGTTCATGTTCAAGCTTCAATTTCTCCATGTGACCAAGTGCCATACATTGGCCGAAAAGGAACACCCACCCAAAATATTTTGGCTATATGCAACTTCAACATGCAATTCACATTTGCATGTGCAGGGTGGGAAGGCAGTGCCCATGACAGTAGAGTATTTTTATCGGCTTTCCGCAATCCTCAATCGAATTTTCCTAAACCCCCAAATGGTAATTTTTTTTTACCTACAGTGCACAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

248

Amino Acids

28.44

Weight (kDa)

6.11

Isoelectric Point (pI)

41.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 36 - 128 4.7e-32 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 167 - 222 1.8e-06 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43722 AT3G30525 AT5G28730
fragaria_vesca FvH4_1g10461 FvH4_1g19401 FvH4_2g04530 FvH4_2g04530 FvH4_2g11121 FvH4_2g13361 FvH4_3g21471 FvH4_3g26161 FvH4_3g29911 FvH4_4g20922 FvH4_4g30624 FvH4_5g23912 FvH4_6g30381 FvH4_6g53026 FvH4_7g00061 FvH4_7g19211 FvH4_7g19211
malus_domestica MD06G1090500.v1.1 MD08G1193900.v1.1 MD11G1151600.v1.1 MD16G1281600.v1.1
prunus_persica Prupe.1G096200_v2.0.a1 Prupe.1G189200_v2.0.a1 Prupe.1G198400_v2.0.a1 Prupe.6G167200_v2.0.a1 Prupe.6G192900_v2.0.a1
pyrus_communis pycom02g00900 pycom06g03500 pycom07g13740 pycom11g10040 pycom17g21680 pycom520g00090
rosa_chinensis RchiOBHm_Chr1g0354781 RchiOBHm_Chr2g0085491 RchiOBHm_Chr2g0103971 RchiOBHm_Chr2g0145791 RchiOBHm_Chr4g0414051 RchiOBHm_Chr4g0419691 RchiOBHm_Chr4g0435871 RchiOBHm_Chr6g0292381 RchiOBHm_Chr7g0199291
rosa_laevigata RLG00000035604
rosa_multiflora Rmu_co8287949.1_g000001 Rmu_sc0000079.1_g000057 Rmu_sc0000240.1_g000063 Rmu_sc0000552.1_g000020 Rmu_sc0000950.1_g000002 Rmu_sc0001257.1_g000003 Rmu_sc0001597.1_g000014 Rmu_sc0001939.1_g000006 Rmu_sc0002160.1_g000015 Rmu_sc0003526.1_g000015 Rmu_sc0005712.1_g000025 Rmu_sc0006032.1_g000011 Rmu_sc0006935.1_g000005 Rmu_sc0008636.1_g000002 Rmu_sc0009205.1_g000006 Rmu_sc0009438.1_g000007 Rmu_ssc0000486.1_g000013
rosa_roxburghii Rroxscaffold_1G00003870 Rroxscaffold_1G00014970 Rroxscaffold_3G00218750 Rroxscaffold_5G00367160 Rroxscaffold_7G00186480
rosa_rugosa Rorug05G0192000
rosa_samantha Rh4BG219200
rosa_wichuraiana Rw1G010350 Rw1G014480 Rw1G023690 Rw1G033480 Rw2G050290 Rw3G027480 Rw4G033650 Rw5G005670 Rw5G021010 Rw5G021860 Rw5G041260 Rw5G041760 Rw6G008410 Rw6G021080 Rw6G022950 Rw6G025480 Rw6G028700 Rw7G002710 Rw7G004940 Rw7G031510 Rw7G041110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 171
AccB7I CCANNNNNTGG 1 cut(s) 554
AciI CCGC 1 cut(s) 679
AclI AACGTT 1 cut(s) 334
AclWI GGATC 1 cut(s) 413
AcoI YGGCCR 1 cut(s) 556
AcsI RAATTY 3 cut(s) 425, 447, 694
AfaI GTAC 2 cut(s) 83, 256
AfiI CCNNNNNNNGG 2 cut(s) 554, 713
AluBI AGCT 3 cut(s) 111, 272, 521
AluI AGCT 3 cut(s) 111, 272, 521
Alw21I GWGCWC 2 cut(s) 405, 741
Alw26I GTCTC 2 cut(s) 282, 338
Alw44I GTGCAC 1 cut(s) 737
AlwI GGATC 1 cut(s) 413
AoxI GGCC 1 cut(s) 556
ApaLI GTGCAC 1 cut(s) 737
ApoI RAATTY 3 cut(s) 425, 447, 694
AspLEI GCGC 1 cut(s) 497
AsuHPI GGTGA 1 cut(s) 353
BaeGI GKGCMC 2 cut(s) 649, 741
BaeI ACNNNNGTAYC 2 cut(s) 238, 271
Bbv12I GWGCWC 2 cut(s) 405, 741
BccI CCATC 2 cut(s) 178, 497
BcoDI GTCTC 2 cut(s) 282, 338
BfmI CTRYAG 1 cut(s) 732
BfoI RGCGCY 1 cut(s) 498
BmsI GCATC 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 33
BpuEI CTTGAG 2 cut(s) 359, 445
Bsc4I CCNNNNNNNGG 2 cut(s) 554, 713
Bse1I ACTGG 2 cut(s) 55, 175
BseGI GGATG 2 cut(s) 189, 435
BseLI CCNNNNNNNGG 2 cut(s) 554, 713
BseNI ACTGG 2 cut(s) 55, 175
BseRI GAGGAG 2 cut(s) 35, 38
BseSI GKGCMC 2 cut(s) 649, 741
BsgI GTGCAG 1 cut(s) 648
BshFI GGCC 1 cut(s) 558
BsiHKAI GWGCWC 2 cut(s) 405, 741
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 2 cut(s) 554, 713
BsmAI GTCTC 2 cut(s) 282, 338
BsmBI CGTCTC 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 306
BsnI GGCC 1 cut(s) 558
Bsp1286I GDGCHC 3 cut(s) 405, 649, 741
Bsp143I GATC 1 cut(s) 418
BspACI CCGC 1 cut(s) 679
BspANI GGCC 1 cut(s) 558
BspPI GGATC 1 cut(s) 413
BsrI ACTGG 2 cut(s) 55, 175
BssMI GATC 1 cut(s) 418
Bst4CI ACNGT 4 cut(s) 259, 349, 656, 736
BstF5I GGATG 2 cut(s) 189, 435
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 1 cut(s) 421
BstMAI GTCTC 2 cut(s) 282, 338
BstMBI GATC 1 cut(s) 418
BstMWI GCNNNNNNNGC 1 cut(s) 409
BstNSI RCATGY 3 cut(s) 472, 610, 627
BstSFI CTRYAG 1 cut(s) 732
BstSLI GKGCMC 2 cut(s) 649, 741
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BsuRI GGCC 1 cut(s) 558
BtsCI GGATG 2 cut(s) 189, 435
BtsI GCAGTG 1 cut(s) 649
BtsIMutI CAGTG 2 cut(s) 649, 741
CfoI GCGC 1 cut(s) 497
Csp6I GTAC 2 cut(s) 82, 255
CviAII CATG 6 cut(s) 469, 512, 534, 607, 624, 650
CviJI RGCY 8 cut(s) 8, 111, 272, 412, 521, 558, 590, 674
CviKI_1 RGCY 8 cut(s) 8, 111, 272, 412, 521, 558, 590, 674
CviQI GTAC 2 cut(s) 82, 255
DpnI GATC 1 cut(s) 420
DpnII GATC 1 cut(s) 418
EaeI YGGCCR 1 cut(s) 556
Esp3I CGTCTC 1 cut(s) 338
FaeI CATG 6 cut(s) 472, 515, 537, 610, 627, 653
FaqI GGGAC 1 cut(s) 306
FatI CATG 6 cut(s) 468, 511, 533, 606, 623, 649
FokI GGATG 2 cut(s) 196, 422
GlaI GCGC 1 cut(s) 496
GsuI CTGGAG 1 cut(s) 33
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 1 cut(s) 558
HhaI GCGC 1 cut(s) 497
Hin1II CATG 6 cut(s) 472, 515, 537, 610, 627, 653
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HindIII AAGCTT 1 cut(s) 519
HinfI GANTC 1 cut(s) 458
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 1 cut(s) 739
Hpy188I TCNGA 1 cut(s) 90
Hpy188III TCNNGA 5 cut(s) 12, 74, 376, 422, 462
Hpy8I GTNNAC 1 cut(s) 739
HpyAV CCTTC 2 cut(s) 229, 632
HpyCH4III ACNGT 4 cut(s) 259, 349, 656, 736
HpyCH4IV ACGT 1 cut(s) 334
HpyCH4V TGCA 5 cut(s) 597, 610, 623, 629, 739
HpyF10VI GCNNNNNNNGC 1 cut(s) 409
HpySE526I ACGT 1 cut(s) 334
Hsp92II CATG 6 cut(s) 472, 515, 537, 610, 627, 653
HspAI GCGC 1 cut(s) 495
Kzo9I GATC 1 cut(s) 418
LmnI GCTCC 1 cut(s) 116
LpnPI CCDG 6 cut(s) 36, 105, 188, 324, 426, 615
LweI GCATC 1 cut(s) 444
MaeII ACGT 1 cut(s) 334
MaeIII GTNAC 2 cut(s) 46, 536
MalI GATC 1 cut(s) 420
MboI GATC 1 cut(s) 418
MboII GAAGA 2 cut(s) 238, 428
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 3 cut(s) 405, 649, 741
MluCI AATT 6 cut(s) 425, 447, 525, 611, 694, 718
MlyI GAGTC 1 cut(s) 452
MnlI CCTC 5 cut(s) 13, 16, 142, 483, 696
MseI TTAA 3 cut(s) 134, 203, 480
MslI CAYNNNNRTG 1 cut(s) 622
MwoI GCNNNNNNNGC 1 cut(s) 409
NdeII GATC 1 cut(s) 418
NlaIII CATG 6 cut(s) 472, 515, 537, 610, 627, 653
NmuCI GTSAC 1 cut(s) 536
NspI RCATGY 3 cut(s) 472, 610, 627
PflFI GACNNNGTC 1 cut(s) 297
PflMI CCANNNNNTGG 1 cut(s) 554
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
PsiI TTATAA 1 cut(s) 171
Psp1406I AACGTT 1 cut(s) 334
PsuI RGATCY 1 cut(s) 418
PsyI GACNNNGTC 1 cut(s) 297
RsaI GTAC 2 cut(s) 83, 256
RsaNI GTAC 2 cut(s) 82, 255
RseI CAYNNNNRTG 1 cut(s) 622
SaqAI TTAA 3 cut(s) 134, 203, 480
Sau3AI GATC 1 cut(s) 418
SchI GAGTC 1 cut(s) 452
SduI GDGCHC 3 cut(s) 405, 649, 741
SetI ASST 8 cut(s) 113, 124, 167, 274, 301, 337, 523, 733
SfaNI GCATC 1 cut(s) 444
SfcI CTRYAG 1 cut(s) 732
SmiMI CAYNNNNRTG 1 cut(s) 622
SmlI CTYRAG 2 cut(s) 374, 460
SmoI CTYRAG 2 cut(s) 374, 460
Sse9I AATT 6 cut(s) 425, 447, 525, 611, 694, 718
SsiI CCGC 1 cut(s) 679
SspI AATATT 1 cut(s) 583
TaaI ACNGT 4 cut(s) 259, 349, 656, 736
TaiI ACGT 1 cut(s) 337
TaqI TCGA 1 cut(s) 692
TasI AATT 6 cut(s) 425, 447, 525, 611, 694, 718
TatI WGTACW 1 cut(s) 81
Tru1I TTAA 3 cut(s) 134, 203, 480
Tru9I TTAA 3 cut(s) 134, 203, 480
TscAI CASTG 2 cut(s) 649, 741
TseFI GTSAC 1 cut(s) 536
Tsp45I GTSAC 1 cut(s) 536
TspDTI ATGAA 3 cut(s) 171, 459, 500
TspRI CASTG 2 cut(s) 649, 741
Tth111I GACNNNGTC 1 cut(s) 297
Van91I CCANNNNNTGG 1 cut(s) 554
VneI GTGCAC 1 cut(s) 737
XapI RAATTY 3 cut(s) 425, 447, 694
XceI RCATGY 3 cut(s) 472, 610, 627
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.