Rmu_sc0005712.1_g000025
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005712.1
OG0000469
Physical Location & Seq
Forward (+)
135883 .. 136925
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005712.1_g000025.1.cds

Sequence Viewer

Length: 864 bp
atgagaatggctgctgagcgctttcaacggtcaaaagatactattcatcgacaatttaagcgtgtattagcagccttgtgtaagttatcgccacagatcatacgtcctcaaagtcaaggagaaacacctccagaaattttgaacaatccaaagttttatccatatttcgagaaatgtattggggcaattgatggaacacatgtcgctgcatgggccccggctcagaaacaaacttcatatcatggtaggaaggtccttgttacccaaaatgtcatgtgtgcatgctcctttgacatgatgttcacctttgtttacactggatgggaacggactgcgaatgattctagggtctttgcagatgcagtgaccaggccagagaataaatttccatttccaaatgaaggatattattatgtggtggatgctggatatacaaacatgccaggattcttagctccttatcgaggtgagaggtatcatttgcgtgattacagaggacctcgtcgtacaccaagagggcctagagagttgttcaattacagacattcttcattgcgaaatgtgattgagagatgttttggtgtactgaaagcgcgttttccaattttaaaatatatgcctaattatcctccaagaagacaacgacgtatccctattgcatgtcgtgttttacacaattttattcggaaggaagctcgttgcgacagattgtttgaagcttttgacgtagaagatatgatttttgaggaggaaaacagtacatcagcgaacttagatatgtcacaagagaactttgcacaaatggctaatgttagaaatgaaattgcagaagacttgtggcaagattttatccctcacccttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

287

Amino Acids

33.76

Weight (kDa)

9.31

Isoelectric Point (pI)

53.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43722 AT3G30525 AT5G28730
fragaria_vesca FvH4_1g10461 FvH4_1g19401 FvH4_2g04530 FvH4_2g04530 FvH4_2g11121 FvH4_2g13361 FvH4_3g21471 FvH4_3g26161 FvH4_3g29911 FvH4_4g20922 FvH4_4g30624 FvH4_5g23912 FvH4_6g30381 FvH4_6g53026 FvH4_7g00061 FvH4_7g19211 FvH4_7g19211
malus_domestica MD06G1090500.v1.1 MD08G1193900.v1.1 MD11G1151600.v1.1 MD16G1281600.v1.1
prunus_persica Prupe.1G096200_v2.0.a1 Prupe.1G189200_v2.0.a1 Prupe.1G198400_v2.0.a1 Prupe.6G167200_v2.0.a1 Prupe.6G192900_v2.0.a1
pyrus_communis pycom02g00900 pycom06g03500 pycom07g13740 pycom11g10040 pycom17g21680 pycom520g00090
rosa_chinensis RchiOBHm_Chr1g0354781 RchiOBHm_Chr2g0085491 RchiOBHm_Chr2g0103971 RchiOBHm_Chr2g0145791 RchiOBHm_Chr4g0414051 RchiOBHm_Chr4g0419691 RchiOBHm_Chr4g0435871 RchiOBHm_Chr6g0292381 RchiOBHm_Chr7g0199291
rosa_laevigata RLG00000035604
rosa_multiflora Rmu_co8287949.1_g000001 Rmu_sc0000079.1_g000057 Rmu_sc0000240.1_g000063 Rmu_sc0000552.1_g000020 Rmu_sc0000950.1_g000002 Rmu_sc0001257.1_g000003 Rmu_sc0001597.1_g000014 Rmu_sc0001939.1_g000006 Rmu_sc0002160.1_g000015 Rmu_sc0003526.1_g000015 Rmu_sc0005712.1_g000025 Rmu_sc0006032.1_g000011 Rmu_sc0006935.1_g000005 Rmu_sc0008636.1_g000002 Rmu_sc0009205.1_g000006 Rmu_sc0009438.1_g000007 Rmu_ssc0000486.1_g000013
rosa_roxburghii Rroxscaffold_1G00003870 Rroxscaffold_1G00014970 Rroxscaffold_3G00218750 Rroxscaffold_5G00367160 Rroxscaffold_7G00186480
rosa_rugosa Rorug05G0192000
rosa_samantha Rh4BG219200
rosa_wichuraiana Rw1G010350 Rw1G014480 Rw1G023690 Rw1G033480 Rw2G050290 Rw3G027480 Rw4G033650 Rw5G005670 Rw5G021010 Rw5G021860 Rw5G041260 Rw5G041760 Rw6G008410 Rw6G021080 Rw6G022950 Rw6G025480 Rw6G028700 Rw7G002710 Rw7G004940 Rw7G031510 Rw7G041110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 595
AcsI RAATTY 2 cut(s) 135, 383
AfaI GTAC 3 cut(s) 508, 585, 760
AfeI AGCGCT 1 cut(s) 20
AfiI CCNNNNNNNGG 2 cut(s) 401, 464
AflIII ACRYGT 1 cut(s) 199
AgsI TTSAA 4 cut(s) 26, 142, 535, 716
AjnI CCWGG 2 cut(s) 368, 442
AluBI AGCT 3 cut(s) 455, 695, 719
AluI AGCT 3 cut(s) 455, 695, 719
Aor51HI AGCGCT 1 cut(s) 20
AoxI GGCC 3 cut(s) 213, 371, 518
ApaI GGGCCC 1 cut(s) 217
ApeKI GCWGC 3 cut(s) 11, 71, 206
ApoI RAATTY 2 cut(s) 135, 383
ArsI GACNNNNNNTTYG 2 cut(s) 695, 727
AspLEI GCGC 2 cut(s) 21, 595
AspS9I GGNCC 5 cut(s) 213, 214, 253, 497, 518
AsuC2I CCSGG 1 cut(s) 218
AsuHPI GGTGA 3 cut(s) 295, 479, 848
AvaII GGWCC 2 cut(s) 253, 497
BaeGI GKGCMC 1 cut(s) 217
BanII GRGCYC 1 cut(s) 217
BbsI GAAGAC 2 cut(s) 643, 837
BbvI GCAGC 2 cut(s) 83, 193
BccI CCATC 2 cut(s) 185, 315
BciT130I CCWGG 2 cut(s) 370, 444
BciVI GTATCC 1 cut(s) 659
BcnI CCSGG 1 cut(s) 218
BfaI CTAG 2 cut(s) 345, 522
BfoI RGCGCY 1 cut(s) 22
BfuI GTATCC 1 cut(s) 659
BisI GCNGC 3 cut(s) 12, 72, 207
BlpI GCTNAGC 1 cut(s) 15
BlsI GCNGC 3 cut(s) 13, 73, 208
Bme1390I CCNGG 3 cut(s) 218, 370, 444
Bme18I GGWCC 2 cut(s) 253, 497
BmgT120I GGNCC 5 cut(s) 213, 214, 253, 497, 518
BmiI GGNNCC 2 cut(s) 215, 216
BmrFI CCNGG 3 cut(s) 218, 370, 444
BmsI GCATC 2 cut(s) 349, 412
BpiI GAAGAC 2 cut(s) 643, 837
BpmI CTGGAG 1 cut(s) 114
Bpu1102I GCTNAGC 1 cut(s) 15
BpuMI CCSGG 1 cut(s) 218
BsaJI CCNNGG 1 cut(s) 216
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 464
Bse1I ACTGG 1 cut(s) 322
Bse3DI GCAATG 1 cut(s) 551
BseBI CCWGG 2 cut(s) 370, 444
BseDI CCNNGG 1 cut(s) 216
BseGI GGATG 2 cut(s) 326, 427
BseLI CCNNNNNNNGG 2 cut(s) 401, 464
BseMI GCAATG 1 cut(s) 551
BseMII CTCAG 2 cut(s) 6, 236
BseNI ACTGG 1 cut(s) 322
BseRI GAGGAG 1 cut(s) 761
BseSI GKGCMC 1 cut(s) 217
BseXI GCAGC 2 cut(s) 83, 193
Bsh1236I CGCG 1 cut(s) 595
BshFI GGCC 3 cut(s) 215, 373, 520
BsiSI CCGG 1 cut(s) 218
BslI CCNNNNNNNGG 2 cut(s) 401, 464
BsnI GGCC 3 cut(s) 215, 373, 520
Bsp120I GGGCCC 1 cut(s) 213
Bsp1286I GDGCHC 1 cut(s) 217
Bsp143I GATC 1 cut(s) 96
Bsp1720I GCTNAGC 1 cut(s) 15
BspANI GGCC 3 cut(s) 215, 373, 520
BspCNI CTCAG 2 cut(s) 7, 235
BspFNI CGCG 1 cut(s) 595
BspLI GGNNCC 2 cut(s) 215, 216
BsrDI GCAATG 1 cut(s) 551
BsrI ACTGG 1 cut(s) 322
BssECI CCNNGG 1 cut(s) 216
BssMI GATC 1 cut(s) 96
Bst2UI CCWGG 2 cut(s) 370, 444
Bst4CI ACNGT 2 cut(s) 30, 758
BstC8I GCNNGC 1 cut(s) 283
BstDEI CTNAG 5 cut(s) 15, 222, 451, 772, 861
BstENI CCTNNNNNAGG 1 cut(s) 462
BstF5I GGATG 2 cut(s) 326, 427
BstFNI CGCG 1 cut(s) 595
BstH2I RGCGCY 1 cut(s) 22
BstHHI GCGC 2 cut(s) 21, 595
BstKTI GATC 1 cut(s) 99
BstMBI GATC 1 cut(s) 96
BstMWI GCNNNNNNNGC 2 cut(s) 212, 803
BstNI CCWGG 2 cut(s) 370, 444
BstNSI RCATGY 4 cut(s) 203, 285, 442, 663
BstSCI CCNGG 3 cut(s) 216, 368, 442
BstSLI GKGCMC 1 cut(s) 217
BstUI CGCG 1 cut(s) 595
BstV1I GCAGC 2 cut(s) 83, 193
BstV2I GAAGAC 2 cut(s) 643, 837
BsuI GTATCC 1 cut(s) 659
BsuRI GGCC 3 cut(s) 215, 373, 520
BtsCI GGATG 2 cut(s) 326, 427
BtsI GCAGTG 1 cut(s) 369
BtsIMutI CAGTG 2 cut(s) 315, 369
Cac8I GCNNGC 1 cut(s) 283
CfoI GCGC 2 cut(s) 21, 595
Cfr13I GGNCC 5 cut(s) 213, 214, 253, 497, 518
Csp6I GTAC 3 cut(s) 507, 584, 759
CviAII CATG 8 cut(s) 200, 210, 242, 274, 282, 295, 439, 660
CviQI GTAC 3 cut(s) 507, 584, 759
DdeI CTNAG 5 cut(s) 15, 222, 451, 772, 861
DpnI GATC 1 cut(s) 98
DpnII GATC 1 cut(s) 96
DraI TTTAAA 1 cut(s) 609
Eco24I GRGCYC 1 cut(s) 217
Eco47I GGWCC 2 cut(s) 253, 497
Eco47III AGCGCT 1 cut(s) 20
EcoNI CCTNNNNNAGG 1 cut(s) 462
EcoO109I RGGNCCY 4 cut(s) 214, 253, 497, 518
EcoRII CCWGG 2 cut(s) 368, 442
EcoT38I GRGCYC 1 cut(s) 217
FaeI CATG 8 cut(s) 203, 213, 245, 277, 285, 298, 442, 663
FatI CATG 8 cut(s) 199, 209, 241, 273, 281, 294, 438, 659
Fnu4HI GCNGC 3 cut(s) 12, 72, 207
FokI GGATG 2 cut(s) 333, 434
FriOI GRGCYC 1 cut(s) 217
Fsp4HI GCNGC 3 cut(s) 12, 72, 207
FspBI CTAG 2 cut(s) 345, 522
GlaI GCGC 2 cut(s) 20, 594
GluI GCNGC 3 cut(s) 12, 72, 207
GsuI CTGGAG 1 cut(s) 114
HaeII RGCGCY 1 cut(s) 22
HaeIII GGCC 3 cut(s) 215, 373, 520
HapII CCGG 1 cut(s) 218
HhaI GCGC 2 cut(s) 21, 595
Hin1II CATG 8 cut(s) 203, 213, 245, 277, 285, 298, 442, 663
Hin6I GCGC 2 cut(s) 19, 593
HinP1I GCGC 2 cut(s) 19, 593
HindIII AAGCTT 1 cut(s) 717
HinfI GANTC 2 cut(s) 341, 447
HpaII CCGG 1 cut(s) 218
HphI GGTGA 3 cut(s) 295, 479, 848
Hpy166II GTNNAC 4 cut(s) 303, 313, 509, 584
Hpy188I TCNGA 2 cut(s) 225, 687
Hpy188III TCNNGA 2 cut(s) 131, 169
Hpy8I GTNNAC 4 cut(s) 303, 313, 509, 584
Hpy99I CGWCG 2 cut(s) 507, 648
HpyAV CCTTC 3 cut(s) 244, 395, 682
HpyCH4III ACNGT 2 cut(s) 30, 758
HpyCH4IV ACGT 3 cut(s) 103, 646, 726
HpyCH4V TGCA 7 cut(s) 209, 281, 356, 362, 659, 797, 827
HpyF10VI GCNNNNNNNGC 2 cut(s) 212, 803
HpyF3I CTNAG 5 cut(s) 15, 222, 451, 772, 861
HpySE526I ACGT 3 cut(s) 103, 646, 726
Hsp92II CATG 8 cut(s) 203, 213, 245, 277, 285, 298, 442, 663
HspAI GCGC 2 cut(s) 19, 593
Kzo9I GATC 1 cut(s) 96
LmnI GCTCC 2 cut(s) 290, 460
LpnPI CCDG 9 cut(s) 144, 231, 303, 355, 382, 387, 411, 429, 456
Lsp1109I GCAGC 2 cut(s) 83, 193
LweI GCATC 2 cut(s) 349, 412
MaeI CTAG 2 cut(s) 345, 522
MaeII ACGT 3 cut(s) 103, 646, 726
MaeIII GTNAC 3 cut(s) 259, 364, 780
MalI GATC 1 cut(s) 98
MboI GATC 1 cut(s) 96
MboII GAAGA 4 cut(s) 540, 648, 743, 842
MfeI CAATTG 1 cut(s) 186
MhlI GDGCHC 1 cut(s) 217
MluCI AATT 9 cut(s) 53, 135, 186, 383, 535, 603, 622, 676, 822
MseI TTAA 2 cut(s) 57, 608
MslI CAYNNNNRTG 1 cut(s) 483
MspI CCGG 1 cut(s) 218
MspR9I CCNGG 3 cut(s) 218, 370, 444
MunI CAATTG 1 cut(s) 186
MvaI CCWGG 2 cut(s) 370, 444
MvnI CGCG 1 cut(s) 595
MwoI GCNNNNNNNGC 2 cut(s) 212, 803
NciI CCSGG 1 cut(s) 218
NdeII GATC 1 cut(s) 96
NlaIII CATG 8 cut(s) 203, 213, 245, 277, 285, 298, 442, 663
NlaIV GGNNCC 2 cut(s) 215, 216
NmuCI GTSAC 2 cut(s) 364, 780
NspI RCATGY 4 cut(s) 203, 285, 442, 663
PaeI GCATGC 1 cut(s) 285
PciI ACATGT 1 cut(s) 199
PfeI GAWTC 2 cut(s) 341, 447
PflFI GACNNNGTC 1 cut(s) 501
PkrI GCNGC 3 cut(s) 13, 73, 208
PpuMI RGGWCCY 2 cut(s) 253, 497
PscI ACATGT 1 cut(s) 199
Psp5II RGGWCCY 2 cut(s) 253, 497
Psp6I CCWGG 2 cut(s) 368, 442
PspGI CCWGG 2 cut(s) 368, 442
PspN4I GGNNCC 2 cut(s) 215, 216
PspOMI GGGCCC 1 cut(s) 213
PspPI GGNCC 5 cut(s) 213, 214, 253, 497, 518
PspPPI RGGWCCY 2 cut(s) 253, 497
PsyI GACNNNGTC 1 cut(s) 501
RsaI GTAC 3 cut(s) 508, 585, 760
RsaNI GTAC 3 cut(s) 507, 584, 759
RseI CAYNNNNRTG 1 cut(s) 483
SaqAI TTAA 2 cut(s) 57, 608
SatI GCNGC 3 cut(s) 12, 72, 207
Sau3AI GATC 1 cut(s) 96
Sau96I GGNCC 5 cut(s) 213, 214, 253, 497, 518
ScrFI CCNGG 3 cut(s) 218, 370, 444
SduI GDGCHC 1 cut(s) 217
SfaNI GCATC 2 cut(s) 349, 412
SinI GGWCC 2 cut(s) 253, 497
SmiMI CAYNNNNRTG 1 cut(s) 483
SphI GCATGC 1 cut(s) 285
Sse9I AATT 9 cut(s) 53, 135, 186, 383, 535, 603, 622, 676, 822
SspMI CTAG 2 cut(s) 345, 522
StyD4I CCNGG 3 cut(s) 216, 368, 442
TaaI ACNGT 2 cut(s) 30, 758
TaiI ACGT 3 cut(s) 106, 649, 729
TaqI TCGA 3 cut(s) 49, 168, 463
TasI AATT 9 cut(s) 53, 135, 186, 383, 535, 603, 622, 676, 822
TatI WGTACW 2 cut(s) 583, 758
TfiI GAWTC 2 cut(s) 341, 447
Tru1I TTAA 2 cut(s) 57, 608
Tru9I TTAA 2 cut(s) 57, 608
TscAI CASTG 2 cut(s) 322, 369
TseFI GTSAC 2 cut(s) 364, 780
TseI GCWGC 3 cut(s) 11, 71, 206
Tsp45I GTSAC 2 cut(s) 364, 780
TspDTI ATGAA 5 cut(s) 35, 225, 414, 540, 834
TspGWI ACGGA 1 cut(s) 343
TspRI CASTG 2 cut(s) 322, 369
Tth111I GACNNNGTC 1 cut(s) 501
VpaK11BI GGWCC 2 cut(s) 253, 497
XagI CCTNNNNNAGG 1 cut(s) 462
XapI RAATTY 2 cut(s) 135, 383
XceI RCATGY 4 cut(s) 203, 285, 442, 663
XspI CTAG 2 cut(s) 345, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.