pycom06g03500
MYB Family

nuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0000469
Physical Location & Seq
Reverse (-)
4431090 .. 4431506
417 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g03500.1

Sequence Viewer

Length: 417 bp
ATGAGAGATTATTTGGGACCATATAAAGGTGAAAGATATCATCTTCCGGATTTTTGTAGGGGTCCCAAACCAACGGGTCATAAAGAGGTATTCAACCACATGCATTCTTCTCTTCGGAGCATCATTGAACGAACTTTTGGGGTATGGAAGAAAAGATGGGCAATTTTAAGGGATATGCCTAATTACCCGTTTAATAAACAAGTGAAGATTGTCATTACTACAATGGCTCTTCATAACTACATACGGAGGTATTCTGAACGTGATTGTCATTTTGATGACCCCAGGAACTATTGTGAAGAGAGCGATAGTAGTGATGATGATGATGAAGAATACCAAAATTATCAAGTTGAAGGATCCCATGAGATAGAAGCATTAAGAAATAGAATAACGGTAAATTTGATGAATGCATCTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4 Myb_DNA-bind_3

Protein Analysis

139

Amino Acids

16.55

Weight (kDa)

6.37

Isoelectric Point (pI)

63.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 20 - 79 7.6e-08 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43722 AT3G30525 AT5G28730
fragaria_vesca FvH4_1g10461 FvH4_1g19401 FvH4_2g04530 FvH4_2g04530 FvH4_2g11121 FvH4_2g13361 FvH4_3g21471 FvH4_3g26161 FvH4_3g29911 FvH4_4g20922 FvH4_4g30624 FvH4_5g23912 FvH4_6g30381 FvH4_6g53026 FvH4_7g00061 FvH4_7g19211 FvH4_7g19211
malus_domestica MD06G1090500.v1.1 MD08G1193900.v1.1 MD11G1151600.v1.1 MD16G1281600.v1.1
prunus_persica Prupe.1G096200_v2.0.a1 Prupe.1G189200_v2.0.a1 Prupe.1G198400_v2.0.a1 Prupe.6G167200_v2.0.a1 Prupe.6G192900_v2.0.a1
pyrus_communis pycom02g00900 pycom06g03500 pycom07g13740 pycom11g10040 pycom17g21680 pycom520g00090
rosa_chinensis RchiOBHm_Chr1g0354781 RchiOBHm_Chr2g0085491 RchiOBHm_Chr2g0103971 RchiOBHm_Chr2g0145791 RchiOBHm_Chr4g0414051 RchiOBHm_Chr4g0419691 RchiOBHm_Chr4g0435871 RchiOBHm_Chr6g0292381 RchiOBHm_Chr7g0199291
rosa_laevigata RLG00000035604
rosa_multiflora Rmu_co8287949.1_g000001 Rmu_sc0000079.1_g000057 Rmu_sc0000240.1_g000063 Rmu_sc0000552.1_g000020 Rmu_sc0000950.1_g000002 Rmu_sc0001257.1_g000003 Rmu_sc0001597.1_g000014 Rmu_sc0001939.1_g000006 Rmu_sc0002160.1_g000015 Rmu_sc0003526.1_g000015 Rmu_sc0005712.1_g000025 Rmu_sc0006032.1_g000011 Rmu_sc0006935.1_g000005 Rmu_sc0008636.1_g000002 Rmu_sc0009205.1_g000006 Rmu_sc0009438.1_g000007 Rmu_ssc0000486.1_g000013
rosa_roxburghii Rroxscaffold_1G00003870 Rroxscaffold_1G00014970 Rroxscaffold_3G00218750 Rroxscaffold_5G00367160 Rroxscaffold_7G00186480
rosa_rugosa Rorug05G0192000
rosa_samantha Rh4BG219200
rosa_wichuraiana Rw1G010350 Rw1G014480 Rw1G023690 Rw1G033480 Rw2G050290 Rw3G027480 Rw4G033650 Rw5G005670 Rw5G021010 Rw5G021860 Rw5G041260 Rw5G041760 Rw6G008410 Rw6G021080 Rw6G022950 Rw6G025480 Rw6G028700 Rw7G002710 Rw7G004940 Rw7G031510 Rw7G041110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 46
AclWI GGATC 2 cut(s) 348, 361
AcsI RAATTY 1 cut(s) 394
AfiI CCNNNNNNNGG 1 cut(s) 26
AgsI TTSAA 3 cut(s) 94, 128, 350
AjnI CCWGG 1 cut(s) 281
AjuI GAANNNNNNNTTGG 2 cut(s) 120, 152
AlwI GGATC 2 cut(s) 348, 361
Aor13HI TCCGGA 1 cut(s) 46
ApoI RAATTY 1 cut(s) 394
AspS9I GGNCC 2 cut(s) 17, 62
AsuHPI GGTGA 1 cut(s) 41
AvaII GGWCC 2 cut(s) 17, 62
BamHI GGATCC 1 cut(s) 353
BccI CCATC 1 cut(s) 150
BciT130I CCWGG 1 cut(s) 283
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 2 cut(s) 17, 62
BmgT120I GGNCC 2 cut(s) 17, 62
BmiI GGNNCC 4 cut(s) 18, 63, 64, 355
BmrFI CCNGG 1 cut(s) 283
BmsI GCATC 1 cut(s) 129
BsaJI CCNNGG 1 cut(s) 281
BsaWI WCCGGW 1 cut(s) 46
Bsc4I CCNNNNNNNGG 1 cut(s) 26
BseAI TCCGGA 1 cut(s) 46
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 1 cut(s) 281
BseLI CCNNNNNNNGG 1 cut(s) 26
BsiSI CCGG 1 cut(s) 47
BslFI GGGAC 2 cut(s) 30, 48
BslI CCNNNNNNNGG 1 cut(s) 26
BsmFI GGGAC 2 cut(s) 30, 48
BsmI GAATGC 2 cut(s) 103, 409
Bsp13I TCCGGA 1 cut(s) 46
Bsp143I GATC 1 cut(s) 353
BspEI TCCGGA 1 cut(s) 46
BspLI GGNNCC 4 cut(s) 18, 63, 64, 355
BspPI GGATC 2 cut(s) 348, 361
BspQI GCTCTTC 1 cut(s) 234
BssECI CCNNGG 1 cut(s) 281
BssMI GATC 1 cut(s) 353
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 1 cut(s) 391
Bst6I CTCTTC 3 cut(s) 117, 234, 291
BstKTI GATC 1 cut(s) 356
BstMBI GATC 1 cut(s) 353
BstNI CCWGG 1 cut(s) 283
BstNSI RCATGY 1 cut(s) 103
BstSCI CCNGG 1 cut(s) 281
BstX2I RGATCY 1 cut(s) 353
BstYI RGATCY 1 cut(s) 353
Cfr13I GGNCC 2 cut(s) 17, 62
CviAII CATG 2 cut(s) 100, 359
CviJI RGCY 1 cut(s) 227
CviKI_1 RGCY 1 cut(s) 227
DpnI GATC 1 cut(s) 355
DpnII GATC 1 cut(s) 353
Eam1104I CTCTTC 3 cut(s) 117, 234, 291
EarI CTCTTC 3 cut(s) 117, 234, 291
Eco32I GATATC 1 cut(s) 38
Eco47I GGWCC 2 cut(s) 17, 62
EcoO109I RGGNCCY 1 cut(s) 62
EcoRII CCWGG 1 cut(s) 281
EcoRV GATATC 1 cut(s) 38
EcoT22I ATGCAT 2 cut(s) 105, 409
FaeI CATG 2 cut(s) 103, 362
FaiI YATR 9 cut(s) 22, 24, 81, 101, 145, 176, 234, 242, 360
FaqI GGGAC 2 cut(s) 30, 48
FatI CATG 2 cut(s) 99, 358
HapII CCGG 1 cut(s) 47
Hin1II CATG 2 cut(s) 103, 362
HpaII CCGG 1 cut(s) 47
HphI GGTGA 1 cut(s) 41
Hpy188I TCNGA 2 cut(s) 117, 256
Hpy188III TCNNGA 1 cut(s) 47
HpyAV CCTTC 1 cut(s) 344
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 1 cut(s) 259
HpyCH4V TGCA 2 cut(s) 103, 407
HpySE526I ACGT 1 cut(s) 259
Hsp92II CATG 2 cut(s) 103, 362
KflI GGGWCCC 1 cut(s) 62
Kpn2I TCCGGA 1 cut(s) 46
Kzo9I GATC 1 cut(s) 353
LguI GCTCTTC 1 cut(s) 234
LmnI GCTCC 1 cut(s) 117
LpnPI CCDG 3 cut(s) 60, 268, 295
LweI GCATC 1 cut(s) 129
MaeII ACGT 1 cut(s) 259
MalI GATC 1 cut(s) 355
MboI GATC 1 cut(s) 353
MboII GAAGA 8 cut(s) 35, 99, 104, 160, 217, 221, 308, 338
MflI RGATCY 1 cut(s) 353
MluCI AATT 5 cut(s) 162, 181, 337, 394, 412
MnlI CCTC 2 cut(s) 79, 240
Mph1103I ATGCAT 2 cut(s) 105, 409
MroI TCCGGA 1 cut(s) 46
MseI TTAA 3 cut(s) 167, 192, 374
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 1 cut(s) 47
MspR9I CCNGG 1 cut(s) 283
Mva1269I GAATGC 2 cut(s) 103, 409
MvaI CCWGG 1 cut(s) 283
NdeII GATC 1 cut(s) 353
NlaIII CATG 2 cut(s) 103, 362
NlaIV GGNNCC 4 cut(s) 18, 63, 64, 355
NsiI ATGCAT 2 cut(s) 105, 409
NspI RCATGY 1 cut(s) 103
PciSI GCTCTTC 1 cut(s) 234
PctI GAATGC 2 cut(s) 103, 409
PpuMI RGGWCCY 1 cut(s) 62
Psp5II RGGWCCY 1 cut(s) 62
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 4 cut(s) 18, 63, 64, 355
PspPI GGNCC 2 cut(s) 17, 62
PspPPI RGGWCCY 1 cut(s) 62
PsuI RGATCY 1 cut(s) 353
RseI CAYNNNNRTG 1 cut(s) 273
SapI GCTCTTC 1 cut(s) 234
SaqAI TTAA 3 cut(s) 167, 192, 374
Sau3AI GATC 1 cut(s) 353
Sau96I GGNCC 2 cut(s) 17, 62
ScrFI CCNGG 1 cut(s) 283
SetI ASST 4 cut(s) 31, 90, 251, 262
SfaNI GCATC 1 cut(s) 129
SinI GGWCC 2 cut(s) 17, 62
SmiMI CAYNNNNRTG 1 cut(s) 273
Sse9I AATT 5 cut(s) 162, 181, 337, 394, 412
StyD4I CCNGG 1 cut(s) 281
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 1 cut(s) 262
TasI AATT 5 cut(s) 162, 181, 337, 394, 412
Tru1I TTAA 3 cut(s) 167, 192, 374
Tru9I TTAA 3 cut(s) 167, 192, 374
TspDTI ATGAA 3 cut(s) 221, 339, 416
TspGWI ACGGA 1 cut(s) 259
VpaK11BI GGWCC 2 cut(s) 17, 62
XapI RAATTY 1 cut(s) 394
XceI RCATGY 1 cut(s) 103
Zsp2I ATGCAT 2 cut(s) 105, 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.