DDE superfamily endonuclease
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 459 |
| AccBSI | CCGCTC | 1 cut(s) | 741 |
| AccI | GTMKAC | 2 cut(s) | 1225, 1339 |
| AccII | CGCG | 1 cut(s) | 1532 |
| AciI | CCGC | 5 cut(s) | 108, 131, 509, 741, 1532 |
| AclWI | GGATC | 1 cut(s) | 805 |
| AcoI | YGGCCR | 2 cut(s) | 34, 105 |
| AcsI | RAATTY | 3 cut(s) | 473, 1132, 1515 |
| AdeI | CACNNNGTG | 1 cut(s) | 1499 |
| AfaI | GTAC | 1 cut(s) | 271 |
| AfeI | AGCGCT | 1 cut(s) | 252 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 126, 548 |
| AflIII | ACRYGT | 2 cut(s) | 1217, 1440 |
| AjnI | CCWGG | 1 cut(s) | 719 |
| AluBI | AGCT | 2 cut(s) | 345, 1082 |
| AluI | AGCT | 2 cut(s) | 345, 1082 |
| Alw26I | GTCTC | 2 cut(s) | 1322, 1532 |
| AlwI | GGATC | 1 cut(s) | 805 |
| Aor51HI | AGCGCT | 1 cut(s) | 252 |
| AoxI | GGCC | 5 cut(s) | 34, 105, 117, 144, 1473 |
| ApeKI | GCWGC | 2 cut(s) | 1082, 1141 |
| ApoI | RAATTY | 3 cut(s) | 473, 1132, 1515 |
| AseI | ATTAAT | 1 cut(s) | 926 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 851 |
| AspLEI | GCGC | 1 cut(s) | 253 |
| AspS9I | GGNCC | 2 cut(s) | 539, 1474 |
| AsuC2I | CCSGG | 1 cut(s) | 569 |
| AsuHPI | GGTGA | 4 cut(s) | 563, 891, 1044, 1288 |
| AvaII | GGWCC | 1 cut(s) | 539 |
| BalI | TGGCCA | 1 cut(s) | 36 |
| BbsI | GAAGAC | 1 cut(s) | 1426 |
| BbvI | GCAGC | 2 cut(s) | 1069, 1153 |
| BccI | CCATC | 4 cut(s) | 45, 588, 1155, 1303 |
| BceAI | ACGGC | 2 cut(s) | 153, 849 |
| BciT130I | CCWGG | 1 cut(s) | 721 |
| BciVI | GTATCC | 1 cut(s) | 506 |
| BcnI | CCSGG | 1 cut(s) | 569 |
| BcoDI | GTCTC | 2 cut(s) | 1322, 1532 |
| BfaI | CTAG | 5 cut(s) | 41, 342, 938, 1137, 1400 |
| BfoI | RGCGCY | 1 cut(s) | 254 |
| BfuI | GTATCC | 1 cut(s) | 506 |
| BisI | GCNGC | 3 cut(s) | 108, 1083, 1142 |
| BlsI | GCNGC | 3 cut(s) | 109, 1084, 1143 |
| Bme1390I | CCNGG | 2 cut(s) | 569, 721 |
| Bme18I | GGWCC | 1 cut(s) | 539 |
| BmgT120I | GGNCC | 2 cut(s) | 539, 1474 |
| BmiI | GGNNCC | 1 cut(s) | 540 |
| BmrFI | CCNGG | 2 cut(s) | 569, 721 |
| BmsI | GCATC | 2 cut(s) | 496, 1342 |
| BpiI | GAAGAC | 1 cut(s) | 1426 |
| Bpu10I | CCTNAGC | 1 cut(s) | 141 |
| BpuMI | CCSGG | 1 cut(s) | 569 |
| BsaI | GGTCTC | 1 cut(s) | 1322 |
| BsaJI | CCNNGG | 2 cut(s) | 719, 720 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 126, 548 |
| Bse118I | RCCGGY | 1 cut(s) | 202 |
| Bse1I | ACTGG | 1 cut(s) | 1549 |
| Bse3DI | GCAATG | 2 cut(s) | 651, 1359 |
| BseBI | CCWGG | 1 cut(s) | 721 |
| BseDI | CCNNGG | 2 cut(s) | 719, 720 |
| BseGI | GGATG | 1 cut(s) | 1166 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 126, 548 |
| BseMI | GCAATG | 2 cut(s) | 651, 1359 |
| BseMII | CTCAG | 2 cut(s) | 674, 1344 |
| BseNI | ACTGG | 1 cut(s) | 1549 |
| BseXI | GCAGC | 2 cut(s) | 1069, 1153 |
| Bsh1236I | CGCG | 1 cut(s) | 1532 |
| BshFI | GGCC | 5 cut(s) | 36, 107, 119, 146, 1475 |
| BsiSI | CCGG | 2 cut(s) | 203, 569 |
| BslFI | GGGAC | 2 cut(s) | 552, 737 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 126, 548 |
| BsmAI | GTCTC | 2 cut(s) | 1322, 1532 |
| BsmFI | GGGAC | 2 cut(s) | 552, 737 |
| BsnI | GGCC | 5 cut(s) | 36, 107, 119, 146, 1475 |
| Bso31I | GGTCTC | 1 cut(s) | 1322 |
| Bsp1407I | TGTACA | 1 cut(s) | 269 |
| Bsp143I | GATC | 2 cut(s) | 93, 797 |
| BspACI | CCGC | 5 cut(s) | 108, 131, 509, 741, 1532 |
| BspANI | GGCC | 5 cut(s) | 36, 107, 119, 146, 1475 |
| BspCNI | CTCAG | 2 cut(s) | 675, 1345 |
| BspFNI | CGCG | 1 cut(s) | 1532 |
| BspLI | GGNNCC | 1 cut(s) | 540 |
| BspPI | GGATC | 1 cut(s) | 805 |
| BspQI | GCTCTTC | 1 cut(s) | 672 |
| BspTNI | GGTCTC | 1 cut(s) | 1322 |
| BsrBI | CCGCTC | 1 cut(s) | 741 |
| BsrDI | GCAATG | 2 cut(s) | 651, 1359 |
| BsrFI | RCCGGY | 1 cut(s) | 202 |
| BsrGI | TGTACA | 1 cut(s) | 269 |
| BsrI | ACTGG | 1 cut(s) | 1549 |
| BssAI | RCCGGY | 1 cut(s) | 202 |
| BssECI | CCNNGG | 2 cut(s) | 719, 720 |
| BssMI | GATC | 2 cut(s) | 93, 797 |
| BssNAI | GTATAC | 1 cut(s) | 1340 |
| Bst1107I | GTATAC | 1 cut(s) | 1340 |
| Bst2UI | CCWGG | 1 cut(s) | 721 |
| Bst4CI | ACNGT | 1 cut(s) | 274 |
| Bst6I | CTCTTC | 2 cut(s) | 672, 729 |
| BstAUI | TGTACA | 1 cut(s) | 269 |
| BstDEI | CTNAG | 3 cut(s) | 141, 683, 1353 |
| BstF5I | GGATG | 1 cut(s) | 1166 |
| BstFNI | CGCG | 1 cut(s) | 1532 |
| BstH2I | RGCGCY | 1 cut(s) | 254 |
| BstHHI | GCGC | 1 cut(s) | 253 |
| BstKTI | GATC | 2 cut(s) | 96, 800 |
| BstMAI | GTCTC | 2 cut(s) | 1322, 1532 |
| BstMBI | GATC | 2 cut(s) | 93, 797 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 116, 137, 662 |
| BstNI | CCWGG | 1 cut(s) | 721 |
| BstNSI | RCATGY | 3 cut(s) | 1173, 1221, 1444 |
| BstSCI | CCNGG | 2 cut(s) | 567, 719 |
| BstUI | CGCG | 1 cut(s) | 1532 |
| BstV1I | GCAGC | 2 cut(s) | 1069, 1153 |
| BstV2I | GAAGAC | 1 cut(s) | 1426 |
| BstZ17I | GTATAC | 1 cut(s) | 1340 |
| BsuI | GTATCC | 1 cut(s) | 506 |
| BsuRI | GGCC | 5 cut(s) | 36, 107, 119, 146, 1475 |
| BtsCI | GGATG | 1 cut(s) | 1166 |
| CfoI | GCGC | 1 cut(s) | 253 |
| Cfr10I | RCCGGY | 1 cut(s) | 202 |
| Cfr13I | GGNCC | 2 cut(s) | 539, 1474 |
| Csp6I | GTAC | 1 cut(s) | 270 |
| CviAII | CATG | 6 cut(s) | 103, 227, 803, 1170, 1218, 1441 |
| CviQI | GTAC | 1 cut(s) | 270 |
| DdeI | CTNAG | 3 cut(s) | 141, 683, 1353 |
| DpnI | GATC | 2 cut(s) | 95, 799 |
| DpnII | GATC | 2 cut(s) | 93, 797 |
| DraIII | CACNNNGTG | 1 cut(s) | 1499 |
| EaeI | YGGCCR | 2 cut(s) | 34, 105 |
| Eam1104I | CTCTTC | 2 cut(s) | 672, 729 |
| EarI | CTCTTC | 2 cut(s) | 672, 729 |
| Eco31I | GGTCTC | 1 cut(s) | 1322 |
| Eco32I | GATATC | 1 cut(s) | 560 |
| Eco47I | GGWCC | 1 cut(s) | 539 |
| Eco47III | AGCGCT | 1 cut(s) | 252 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1474 |
| EcoRII | CCWGG | 1 cut(s) | 719 |
| EcoRV | GATATC | 1 cut(s) | 560 |
| FaeI | CATG | 6 cut(s) | 106, 230, 806, 1173, 1221, 1444 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1289, 1321 |
| FaqI | GGGAC | 2 cut(s) | 552, 737 |
| FatI | CATG | 6 cut(s) | 102, 226, 802, 1169, 1217, 1440 |
| FauI | CCCGC | 2 cut(s) | 502, 1525 |
| FblI | GTMKAC | 2 cut(s) | 1225, 1339 |
| Fnu4HI | GCNGC | 3 cut(s) | 108, 1083, 1142 |
| FokI | GGATG | 1 cut(s) | 1173 |
| Fsp4HI | GCNGC | 3 cut(s) | 108, 1083, 1142 |
| FspBI | CTAG | 5 cut(s) | 41, 342, 938, 1137, 1400 |
| GlaI | GCGC | 1 cut(s) | 252 |
| GluI | GCNGC | 3 cut(s) | 108, 1083, 1142 |
| HaeII | RGCGCY | 1 cut(s) | 254 |
| HaeIII | GGCC | 5 cut(s) | 36, 107, 119, 146, 1475 |
| HapII | CCGG | 2 cut(s) | 203, 569 |
| HhaI | GCGC | 1 cut(s) | 253 |
| Hin1II | CATG | 6 cut(s) | 106, 230, 806, 1173, 1221, 1444 |
| Hin6I | GCGC | 1 cut(s) | 251 |
| HinP1I | GCGC | 1 cut(s) | 251 |
| HincII | GTYRAC | 1 cut(s) | 913 |
| HindII | GTYRAC | 1 cut(s) | 913 |
| HinfI | GANTC | 4 cut(s) | 190, 338, 847, 1465 |
| HpaI | GTTAAC | 1 cut(s) | 913 |
| HpaII | CCGG | 2 cut(s) | 203, 569 |
| HphI | GGTGA | 4 cut(s) | 563, 891, 1044, 1288 |
| Hpy166II | GTNNAC | 7 cut(s) | 270, 913, 995, 1052, 1226, 1340, 1387 |
| Hpy188I | TCNGA | 2 cut(s) | 55, 288 |
| Hpy188III | TCNNGA | 4 cut(s) | 41, 486, 1306, 1511 |
| Hpy8I | GTNNAC | 7 cut(s) | 270, 913, 995, 1052, 1226, 1340, 1387 |
| HpyAV | CCTTC | 1 cut(s) | 788 |
| HpyCH4III | ACNGT | 1 cut(s) | 274 |
| HpyCH4IV | ACGT | 2 cut(s) | 697, 726 |
| HpyCH4V | TGCA | 5 cut(s) | 4, 300, 381, 1169, 1364 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 116, 137, 662 |
| HpyF3I | CTNAG | 3 cut(s) | 141, 683, 1353 |
| HpySE526I | ACGT | 2 cut(s) | 697, 726 |
| Hsp92II | CATG | 6 cut(s) | 106, 230, 806, 1173, 1221, 1444 |
| HspAI | GCGC | 1 cut(s) | 251 |
| KspAI | GTTAAC | 1 cut(s) | 913 |
| Kzo9I | GATC | 2 cut(s) | 93, 797 |
| LguI | GCTCTTC | 1 cut(s) | 672 |
| LpnPI | CCDG | 9 cut(s) | 112, 136, 216, 279, 582, 706, 733, 1192, 1530 |
| Lsp1109I | GCAGC | 2 cut(s) | 1069, 1153 |
| LweI | GCATC | 2 cut(s) | 496, 1342 |
| MaeI | CTAG | 5 cut(s) | 41, 342, 938, 1137, 1400 |
| MaeII | ACGT | 2 cut(s) | 697, 726 |
| MaeIII | GTNAC | 4 cut(s) | 133, 166, 1174, 1450 |
| MalI | GATC | 2 cut(s) | 95, 799 |
| MbiI | CCGCTC | 1 cut(s) | 741 |
| MboI | GATC | 2 cut(s) | 93, 797 |
| MboII | GAAGA | 7 cut(s) | 257, 598, 655, 659, 746, 782, 1426 |
| MfeI | CAATTG | 1 cut(s) | 333 |
| MlsI | TGGCCA | 1 cut(s) | 36 |
| MluNI | TGGCCA | 1 cut(s) | 36 |
| MnlI | CCTC | 7 cut(s) | 109, 430, 521, 678, 1307, 1348, 1487 |
| Mox20I | TGGCCA | 1 cut(s) | 36 |
| MroXI | GAANNNNTTC | 1 cut(s) | 851 |
| MscI | TGGCCA | 1 cut(s) | 36 |
| MseI | TTAA | 9 cut(s) | 84, 312, 353, 605, 818, 912, 921, 926, 1373 |
| MslI | CAYNNNNRTG | 3 cut(s) | 101, 711, 1445 |
| Msp20I | TGGCCA | 1 cut(s) | 36 |
| MspA1I | CMGCKG | 1 cut(s) | 1082 |
| MspI | CCGG | 2 cut(s) | 203, 569 |
| MspR9I | CCNGG | 2 cut(s) | 569, 721 |
| MunI | CAATTG | 1 cut(s) | 333 |
| MvaI | CCWGG | 1 cut(s) | 721 |
| MvnI | CGCG | 1 cut(s) | 1532 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 116, 137, 662 |
| NciI | CCSGG | 1 cut(s) | 569 |
| NdeII | GATC | 2 cut(s) | 93, 797 |
| NlaIII | CATG | 6 cut(s) | 106, 230, 806, 1173, 1221, 1444 |
| NlaIV | GGNNCC | 1 cut(s) | 540 |
| NmuCI | GTSAC | 1 cut(s) | 133 |
| NspI | RCATGY | 3 cut(s) | 1173, 1221, 1444 |
| PasI | CCCWGGG | 1 cut(s) | 720 |
| PciI | ACATGT | 2 cut(s) | 1217, 1440 |
| PciSI | GCTCTTC | 1 cut(s) | 672 |
| PdmI | GAANNNNTTC | 1 cut(s) | 851 |
| PfeI | GAWTC | 4 cut(s) | 190, 338, 847, 1465 |
| PfoI | TCCNGGA | 1 cut(s) | 567 |
| PkrI | GCNGC | 3 cut(s) | 109, 1084, 1143 |
| PscI | ACATGT | 2 cut(s) | 1217, 1440 |
| PshBI | ATTAAT | 1 cut(s) | 926 |
| PsiI | TTATAA | 1 cut(s) | 459 |
| Psp6I | CCWGG | 1 cut(s) | 719 |
| PspGI | CCWGG | 1 cut(s) | 719 |
| PspN4I | GGNNCC | 1 cut(s) | 540 |
| PspPI | GGNCC | 2 cut(s) | 539, 1474 |
| PvuII | CAGCTG | 1 cut(s) | 1082 |
| RsaI | GTAC | 1 cut(s) | 271 |
| RsaNI | GTAC | 1 cut(s) | 270 |
| RseI | CAYNNNNRTG | 3 cut(s) | 101, 711, 1445 |
| SapI | GCTCTTC | 1 cut(s) | 672 |
| SaqAI | TTAA | 9 cut(s) | 84, 312, 353, 605, 818, 912, 921, 926, 1373 |
| SatI | GCNGC | 3 cut(s) | 108, 1083, 1142 |
| Sau3AI | GATC | 2 cut(s) | 93, 797 |
| Sau96I | GGNCC | 2 cut(s) | 539, 1474 |
| ScrFI | CCNGG | 2 cut(s) | 569, 721 |
| SfaNI | GCATC | 2 cut(s) | 496, 1342 |
| SinI | GGWCC | 1 cut(s) | 539 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 101, 711, 1445 |
| SsiI | CCGC | 5 cut(s) | 108, 131, 509, 741, 1532 |
| SspI | AATATT | 3 cut(s) | 157, 494, 866 |
| SspMI | CTAG | 5 cut(s) | 41, 342, 938, 1137, 1400 |
| StyD4I | CCNGG | 2 cut(s) | 567, 719 |
| TaaI | ACNGT | 1 cut(s) | 274 |
| TaiI | ACGT | 2 cut(s) | 700, 729 |
| TaqI | TCGA | 2 cut(s) | 92, 243 |
| TatI | WGTACW | 1 cut(s) | 269 |
| TauI | GCSGC | 1 cut(s) | 110 |
| TfiI | GAWTC | 4 cut(s) | 190, 338, 847, 1465 |
| Tru1I | TTAA | 9 cut(s) | 84, 312, 353, 605, 818, 912, 921, 926, 1373 |
| Tru9I | TTAA | 9 cut(s) | 84, 312, 353, 605, 818, 912, 921, 926, 1373 |
| TseFI | GTSAC | 1 cut(s) | 133 |
| TseI | GCWGC | 2 cut(s) | 1082, 1141 |
| Tsp45I | GTSAC | 1 cut(s) | 133 |
| TspDTI | ATGAA | 7 cut(s) | 182, 268, 566, 659, 783, 860, 1509 |
| TspGWI | ACGGA | 1 cut(s) | 615 |
| VpaK11BI | GGWCC | 1 cut(s) | 539 |
| VspI | ATTAAT | 1 cut(s) | 926 |
| XapI | RAATTY | 3 cut(s) | 473, 1132, 1515 |
| XbaI | TCTAGA | 1 cut(s) | 40 |
| XceI | RCATGY | 3 cut(s) | 1173, 1221, 1444 |
| XmiI | GTMKAC | 2 cut(s) | 1225, 1339 |
| XmnI | GAANNNNTTC | 1 cut(s) | 851 |
| XspI | CTAG | 5 cut(s) | 41, 342, 938, 1137, 1400 |