RLG00000035604
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000469
Physical Location & Seq
Reverse (-)
69260969 .. 69262356
1388 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035604

Sequence Viewer

Length: 555 bp
ATGGAAGATGGTGACTCAGATAGTTCTTCAGAACTAGATGAAATGGCACAACATATGATAATATGTATGAACATCTATGATTATTGGTCTTCATACATAGACAAGGTTCCTTGCCATACATCGATATTATCTGGAGCTGAATATGTGCAAGAGTTGCTGAATGGACATCCAGATAGAATTTATAACTCATTTCGCATGGATAAACATGTATTTCAAAGGTTGTGTTGCACGCTTGAGAGTTTAAATTTATTAAAAGATGATCGGCATGTAGGCATTCAAGAGGCTGTGGCTATTTTCTTATATATAGTATCTCATAGTGAGCGCATGAGAATGGCTGCTGAGCGCTTTCAACGGTCAAAAGATACTATTCATCGACAATTTAAGCGTGTGTTAGCAGCCTTGTGTAAGTTATCACCACAGATCATACGTCCTCAAAGTCAAGGAGAAACACCTCCAGAAATTTTGAACAATCCAAAGTTTTATCCATATTTCGAGAAGACTTGTGGCAAGACTTTATCCCTCACCCTTAGAAGTTCGGTGATAGTAGATTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

185

Amino Acids

21.34

Weight (kDa)

6.15

Isoelectric Point (pI)

47.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 40 - 136 7.6e-41 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43722 AT3G30525 AT5G28730
fragaria_vesca FvH4_1g10461 FvH4_1g19401 FvH4_2g04530 FvH4_2g04530 FvH4_2g11121 FvH4_2g13361 FvH4_3g21471 FvH4_3g26161 FvH4_3g29911 FvH4_4g20922 FvH4_4g30624 FvH4_5g23912 FvH4_6g30381 FvH4_6g53026 FvH4_7g00061 FvH4_7g19211 FvH4_7g19211
malus_domestica MD06G1090500.v1.1 MD08G1193900.v1.1 MD11G1151600.v1.1 MD16G1281600.v1.1
prunus_persica Prupe.1G096200_v2.0.a1 Prupe.1G189200_v2.0.a1 Prupe.1G198400_v2.0.a1 Prupe.6G167200_v2.0.a1 Prupe.6G192900_v2.0.a1
pyrus_communis pycom02g00900 pycom06g03500 pycom07g13740 pycom11g10040 pycom17g21680 pycom520g00090
rosa_chinensis RchiOBHm_Chr1g0354781 RchiOBHm_Chr2g0085491 RchiOBHm_Chr2g0103971 RchiOBHm_Chr2g0145791 RchiOBHm_Chr4g0414051 RchiOBHm_Chr4g0419691 RchiOBHm_Chr4g0435871 RchiOBHm_Chr6g0292381 RchiOBHm_Chr7g0199291
rosa_laevigata RLG00000035604
rosa_multiflora Rmu_co8287949.1_g000001 Rmu_sc0000079.1_g000057 Rmu_sc0000240.1_g000063 Rmu_sc0000552.1_g000020 Rmu_sc0000950.1_g000002 Rmu_sc0001257.1_g000003 Rmu_sc0001597.1_g000014 Rmu_sc0001939.1_g000006 Rmu_sc0002160.1_g000015 Rmu_sc0003526.1_g000015 Rmu_sc0005712.1_g000025 Rmu_sc0006032.1_g000011 Rmu_sc0006935.1_g000005 Rmu_sc0008636.1_g000002 Rmu_sc0009205.1_g000006 Rmu_sc0009438.1_g000007 Rmu_ssc0000486.1_g000013
rosa_roxburghii Rroxscaffold_1G00003870 Rroxscaffold_1G00014970 Rroxscaffold_3G00218750 Rroxscaffold_5G00367160 Rroxscaffold_7G00186480
rosa_rugosa Rorug05G0192000
rosa_samantha Rh4BG219200
rosa_wichuraiana Rw1G010350 Rw1G014480 Rw1G023690 Rw1G033480 Rw2G050290 Rw3G027480 Rw4G033650 Rw5G005670 Rw5G021010 Rw5G021860 Rw5G041260 Rw5G041760 Rw6G008410 Rw6G021080 Rw6G022950 Rw6G025480 Rw6G028700 Rw7G002710 Rw7G004940 Rw7G031510 Rw7G041110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 183
AcsI RAATTY 3 cut(s) 177, 244, 459
AcuI CTGAAG 1 cut(s) 12
AfeI AGCGCT 1 cut(s) 344
AflIII ACRYGT 1 cut(s) 205
AgsI TTSAA 4 cut(s) 215, 278, 350, 466
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
Aor51HI AGCGCT 1 cut(s) 344
ApeKI GCWGC 2 cut(s) 335, 395
ApoI RAATTY 3 cut(s) 177, 244, 459
AspLEI GCGC 2 cut(s) 324, 345
AsuHPI GGTGA 4 cut(s) 23, 405, 514, 550
BbsI GAAGAC 2 cut(s) 81, 503
BbvI GCAGC 2 cut(s) 322, 407
BccI CCATC 1 cut(s) 2
BfaI CTAG 1 cut(s) 35
BfoI RGCGCY 1 cut(s) 346
BisI GCNGC 2 cut(s) 336, 396
BlpI GCTNAGC 1 cut(s) 339
BlsI GCNGC 2 cut(s) 337, 397
BmiI GGNNCC 1 cut(s) 108
BpiI GAAGAC 2 cut(s) 81, 503
BpmI CTGGAG 2 cut(s) 153, 438
Bpu1102I GCTNAGC 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 122
BseCI ATCGAT 1 cut(s) 122
BseGI GGATG 1 cut(s) 166
BseMII CTCAG 2 cut(s) 30, 330
BseXI GCAGC 2 cut(s) 322, 407
BshVI ATCGAT 1 cut(s) 122
BsmI GAATGC 1 cut(s) 273
Bsp143I GATC 2 cut(s) 259, 420
Bsp1720I GCTNAGC 1 cut(s) 339
BspCNI CTCAG 2 cut(s) 29, 331
BspDI ATCGAT 1 cut(s) 122
BspLI GGNNCC 1 cut(s) 108
BssMI GATC 2 cut(s) 259, 420
Bst4CI ACNGT 1 cut(s) 354
BstAPI GCANNNNNTGC 1 cut(s) 154
BstC8I GCNNGC 1 cut(s) 230
BstDEI CTNAG 3 cut(s) 16, 339, 527
BstF5I GGATG 1 cut(s) 166
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 2 cut(s) 324, 345
BstKTI GATC 2 cut(s) 262, 423
BstMBI GATC 2 cut(s) 259, 420
BstMWI GCNNNNNNNGC 1 cut(s) 154
BstNSI RCATGY 2 cut(s) 209, 269
BstV1I GCAGC 2 cut(s) 322, 407
BstV2I GAAGAC 2 cut(s) 81, 503
Bsu15I ATCGAT 1 cut(s) 122
BsuTUI ATCGAT 1 cut(s) 122
BtsCI GGATG 1 cut(s) 166
Cac8I GCNNGC 1 cut(s) 230
CfoI GCGC 2 cut(s) 324, 345
ClaI ATCGAT 1 cut(s) 122
CviAII CATG 4 cut(s) 196, 206, 266, 325
CviJI RGCY 5 cut(s) 137, 284, 290, 335, 398
CviKI_1 RGCY 5 cut(s) 137, 284, 290, 335, 398
DdeI CTNAG 3 cut(s) 16, 339, 527
DpnI GATC 2 cut(s) 261, 422
DpnII GATC 2 cut(s) 259, 420
DraI TTTAAA 1 cut(s) 243
Eco47III AGCGCT 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 12
FaeI CATG 4 cut(s) 199, 209, 269, 328
FatI CATG 4 cut(s) 195, 205, 265, 324
FauNDI CATATG 1 cut(s) 54
Fnu4HI GCNGC 2 cut(s) 336, 396
FokI GGATG 1 cut(s) 153
Fsp4HI GCNGC 2 cut(s) 336, 396
FspBI CTAG 1 cut(s) 35
GlaI GCGC 2 cut(s) 323, 344
GluI GCNGC 2 cut(s) 336, 396
GsuI CTGGAG 2 cut(s) 153, 438
HaeII RGCGCY 1 cut(s) 346
HhaI GCGC 2 cut(s) 324, 345
Hin1II CATG 4 cut(s) 199, 209, 269, 328
Hin6I GCGC 2 cut(s) 322, 343
HinP1I GCGC 2 cut(s) 322, 343
HinfI GANTC 1 cut(s) 14
HphI GGTGA 4 cut(s) 23, 405, 514, 550
Hpy188I TCNGA 2 cut(s) 19, 31
Hpy188III TCNNGA 5 cut(s) 132, 170, 278, 455, 493
HpyCH4III ACNGT 1 cut(s) 354
HpyCH4IV ACGT 1 cut(s) 427
HpyCH4V TGCA 2 cut(s) 148, 228
HpyF10VI GCNNNNNNNGC 1 cut(s) 154
HpyF3I CTNAG 3 cut(s) 16, 339, 527
HpySE526I ACGT 1 cut(s) 427
Hsp92II CATG 4 cut(s) 199, 209, 269, 328
HspAI GCGC 2 cut(s) 322, 343
Kzo9I GATC 2 cut(s) 259, 420
LmnI GCTCC 1 cut(s) 134
LpnPI CCDG 3 cut(s) 117, 183, 468
Lsp1109I GCAGC 2 cut(s) 322, 407
MaeI CTAG 1 cut(s) 35
MaeII ACGT 1 cut(s) 427
MaeIII GTNAC 1 cut(s) 11
MalI GATC 2 cut(s) 261, 422
MboI GATC 2 cut(s) 259, 420
MboII GAAGA 4 cut(s) 17, 18, 81, 508
MluCI AATT 4 cut(s) 177, 244, 377, 459
MlyI GAGTC 1 cut(s) 8
MnlI CCTC 4 cut(s) 274, 441, 462, 530
MseI TTAA 4 cut(s) 242, 251, 381, 553
MslI CAYNNNNRTG 1 cut(s) 329
Mva1269I GAATGC 1 cut(s) 273
MwoI GCNNNNNNNGC 1 cut(s) 154
NdeI CATATG 1 cut(s) 54
NdeII GATC 2 cut(s) 259, 420
NlaIII CATG 4 cut(s) 199, 209, 269, 328
NlaIV GGNNCC 1 cut(s) 108
NmuCI GTSAC 1 cut(s) 11
NspI RCATGY 2 cut(s) 209, 269
PciI ACATGT 1 cut(s) 205
PctI GAATGC 1 cut(s) 273
PkrI GCNGC 2 cut(s) 337, 397
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
PscI ACATGT 1 cut(s) 205
PsiI TTATAA 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 108
RseI CAYNNNNRTG 1 cut(s) 329
SaqAI TTAA 4 cut(s) 242, 251, 381, 553
SatI GCNGC 2 cut(s) 336, 396
Sau3AI GATC 2 cut(s) 259, 420
SchI GAGTC 1 cut(s) 8
SetI ASST 5 cut(s) 108, 139, 221, 430, 454
SmiMI CAYNNNNRTG 1 cut(s) 329
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 4 cut(s) 177, 244, 377, 459
SspMI CTAG 1 cut(s) 35
TaaI ACNGT 1 cut(s) 354
TaiI ACGT 1 cut(s) 430
TaqI TCGA 3 cut(s) 122, 373, 492
TasI AATT 4 cut(s) 177, 244, 377, 459
Tru1I TTAA 4 cut(s) 242, 251, 381, 553
Tru9I TTAA 4 cut(s) 242, 251, 381, 553
TseFI GTSAC 1 cut(s) 11
TseI GCWGC 2 cut(s) 335, 395
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 4 cut(s) 54, 81, 83, 359
XapI RAATTY 3 cut(s) 177, 244, 459
XceI RCATGY 2 cut(s) 209, 269
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.