Rw4G033650
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000469
Physical Location & Seq
Forward (+)
60774539 .. 60775909
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G033650.1

Sequence Viewer

Length: 1134 bp
ATGGAAGATGGTGACTCTGATAGTTCTTCAGAACTAGATGAAATGGCACAACATATGATAATATGTATGAACATCTATGATTATTGGTCTTCATACATAGACAAGGTTCCTTGCCATACATCGATATTATCTGGAGCTGAATATGTGCAAGAGTTGCTGAATGGACATCCAGATAGAATTTATAACTCATTTCGCATGGATAAACATGTATTTCAAAGGTTGTGTTGCACGCTTGAGAGTTTAAATTTATTAAAAGATGATCGGCATGTAGGCATTCAAGAGGCCGTGGCTATTTTCTTATATATAGTATCTCATAGTGAGCGCATGAGAATGGCTGCTGAGCGCTTTCAACGGTCAAAAGATACTATTCATCGACAATTTAAGCGTGTGTTAGCAGCCTTGTGTAAGTTATCGCCACAGATCATACGTGCTCAAAGTCAAGGAGAAACACCTCCAGAAATTTTGAACAATCCAAAGTTTTATCCATATTTCGAGAAACAAACTTCATATCGTGGTAGGAAGGTCCTTGTTACCCAAAATGTCATGTGTGCATGCTCCTTTGACATGATGTTCACCTTTGTTTATACTAGATGGGAAGGGACTGCGAATGATTCTAGGGTCTTTGCAGATGCAGTGACCAGGCCAGAGAATAAATTTCCATTTCCAAATGAAGGATATTATTATGTGGTGGATGCTGGGTATACAAACATGCCAGGATTCTTAGCTCCTTATCGAGGTGAGAGGTATCATTTGCGTGATTACAGAGGACCTCGTCGTACACCAAGAGGCCCTAGGGAGTTGTTCAATTACAGACATTCTTCATTGCGAAATGTGATTGAGCGATGTTTTGGTGTACTGAAAGCACGTTTTCCAATTTTAAAATATATGCCTAATTATCCTCCAAGAAGACAACAACGTATCCCTATTGCATGTTGTGTTTTACACAATTTTATTCGGAAGGAAGCTCGTCGCGACAGATTGTTTGAAGTTTTTGATGTAGAAGATATGATTTTTGAGGAGGAAAACAGTACACCAGCGAACTTAGATATGTCACAAGAGAACCTTGCACAAATGACTAATGTTAGAAATGAAATTGCAGAAGACTTGTGGCAAAACTTTATCCCTCACCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

377

Amino Acids

44.48

Weight (kDa)

7.59

Isoelectric Point (pI)

49.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 40 - 136 3.6e-40 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 165 - 316 1.9e-20 DDE superfamily endonuclease
Plant_tran PF04827 251 - 322 1.5e-06 Plant transposon protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43722 AT3G30525 AT5G28730
fragaria_vesca FvH4_1g10461 FvH4_1g19401 FvH4_2g04530 FvH4_2g04530 FvH4_2g11121 FvH4_2g13361 FvH4_3g21471 FvH4_3g26161 FvH4_3g29911 FvH4_4g20922 FvH4_4g30624 FvH4_5g23912 FvH4_6g30381 FvH4_6g53026 FvH4_7g00061 FvH4_7g19211 FvH4_7g19211
malus_domestica MD06G1090500.v1.1 MD08G1193900.v1.1 MD11G1151600.v1.1 MD16G1281600.v1.1
prunus_persica Prupe.1G096200_v2.0.a1 Prupe.1G189200_v2.0.a1 Prupe.1G198400_v2.0.a1 Prupe.6G167200_v2.0.a1 Prupe.6G192900_v2.0.a1
pyrus_communis pycom02g00900 pycom06g03500 pycom07g13740 pycom11g10040 pycom17g21680 pycom520g00090
rosa_chinensis RchiOBHm_Chr1g0354781 RchiOBHm_Chr2g0085491 RchiOBHm_Chr2g0103971 RchiOBHm_Chr2g0145791 RchiOBHm_Chr4g0414051 RchiOBHm_Chr4g0419691 RchiOBHm_Chr4g0435871 RchiOBHm_Chr6g0292381 RchiOBHm_Chr7g0199291
rosa_laevigata RLG00000035604
rosa_multiflora Rmu_co8287949.1_g000001 Rmu_sc0000079.1_g000057 Rmu_sc0000240.1_g000063 Rmu_sc0000552.1_g000020 Rmu_sc0000950.1_g000002 Rmu_sc0001257.1_g000003 Rmu_sc0001597.1_g000014 Rmu_sc0001939.1_g000006 Rmu_sc0002160.1_g000015 Rmu_sc0003526.1_g000015 Rmu_sc0005712.1_g000025 Rmu_sc0006032.1_g000011 Rmu_sc0006935.1_g000005 Rmu_sc0008636.1_g000002 Rmu_sc0009205.1_g000006 Rmu_sc0009438.1_g000007 Rmu_ssc0000486.1_g000013
rosa_roxburghii Rroxscaffold_1G00003870 Rroxscaffold_1G00014970 Rroxscaffold_3G00218750 Rroxscaffold_5G00367160 Rroxscaffold_7G00186480
rosa_rugosa Rorug05G0192000
rosa_samantha Rh4BG219200
rosa_wichuraiana Rw1G010350 Rw1G014480 Rw1G023690 Rw1G033480 Rw2G050290 Rw3G027480 Rw4G033650 Rw5G005670 Rw5G021010 Rw5G021860 Rw5G041260 Rw5G041760 Rw6G008410 Rw6G021080 Rw6G022950 Rw6G025480 Rw6G028700 Rw7G002710 Rw7G004940 Rw7G031510 Rw7G041110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 183
AccI GTMKAC 1 cut(s) 701
AccII CGCG 1 cut(s) 972
AcsI RAATTY 4 cut(s) 177, 244, 459, 653
AcuI CTGAAG 1 cut(s) 12
AfaI GTAC 3 cut(s) 778, 855, 1030
AfeI AGCGCT 1 cut(s) 344
AfiI CCNNNNNNNGG 2 cut(s) 671, 734
AflIII ACRYGT 1 cut(s) 205
AgsI TTSAA 6 cut(s) 215, 278, 350, 466, 805, 986
AjnI CCWGG 2 cut(s) 638, 712
AluBI AGCT 3 cut(s) 137, 725, 965
AluI AGCT 3 cut(s) 137, 725, 965
Alw21I GWGCWC 1 cut(s) 433
Aor51HI AGCGCT 1 cut(s) 344
AoxI GGCC 3 cut(s) 282, 641, 787
ApeKI GCWGC 2 cut(s) 335, 395
ApoI RAATTY 4 cut(s) 177, 244, 459, 653
ArsI GACNNNNNNTTYG 2 cut(s) 965, 997
AspA2I CCTAGG 1 cut(s) 791
AspLEI GCGC 2 cut(s) 324, 345
AspS9I GGNCC 3 cut(s) 523, 767, 788
AsuHPI GGTGA 4 cut(s) 23, 565, 749, 1118
AvaII GGWCC 2 cut(s) 523, 767
AvrII CCTAGG 1 cut(s) 791
BbsI GAAGAC 3 cut(s) 81, 913, 1107
Bbv12I GWGCWC 1 cut(s) 433
BbvI GCAGC 2 cut(s) 322, 407
BccI CCATC 2 cut(s) 2, 585
BceAI ACGGC 1 cut(s) 269
BciT130I CCWGG 2 cut(s) 640, 714
BciVI GTATCC 1 cut(s) 929
BfaI CTAG 4 cut(s) 35, 588, 615, 792
BfoI RGCGCY 1 cut(s) 346
BfuI GTATCC 1 cut(s) 929
BisI GCNGC 2 cut(s) 336, 396
BlnI CCTAGG 1 cut(s) 791
BlpI GCTNAGC 1 cut(s) 339
BlsI GCNGC 2 cut(s) 337, 397
Bme1390I CCNGG 2 cut(s) 640, 714
Bme18I GGWCC 2 cut(s) 523, 767
BmgT120I GGNCC 3 cut(s) 523, 767, 788
BmiI GGNNCC 1 cut(s) 108
BmrFI CCNGG 2 cut(s) 640, 714
BmsI GCATC 2 cut(s) 619, 682
BpiI GAAGAC 3 cut(s) 81, 913, 1107
BpmI CTGGAG 2 cut(s) 153, 438
Bpu1102I GCTNAGC 1 cut(s) 339
BpuEI CTTGAG 1 cut(s) 254
Bsa29I ATCGAT 1 cut(s) 122
BsaAI YACGTR 1 cut(s) 428
BsaJI CCNNGG 2 cut(s) 285, 791
Bsc4I CCNNNNNNNGG 2 cut(s) 671, 734
Bse3DI GCAATG 1 cut(s) 821
BseBI CCWGG 2 cut(s) 640, 714
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 2 cut(s) 285, 791
BseGI GGATG 2 cut(s) 166, 697
BseLI CCNNNNNNNGG 2 cut(s) 671, 734
BseMI GCAATG 1 cut(s) 821
BseMII CTCAG 1 cut(s) 330
BseRI GAGGAG 1 cut(s) 1031
BseXI GCAGC 2 cut(s) 322, 407
BseYI CCCAGC 1 cut(s) 695
Bsh1236I CGCG 1 cut(s) 972
BshFI GGCC 3 cut(s) 284, 643, 789
BshVI ATCGAT 1 cut(s) 122
BsiHKAI GWGCWC 1 cut(s) 433
BslFI GGGAC 1 cut(s) 613
BslI CCNNNNNNNGG 2 cut(s) 671, 734
BsmFI GGGAC 1 cut(s) 613
BsmI GAATGC 1 cut(s) 273
BsnI GGCC 3 cut(s) 284, 643, 789
Bsp1286I GDGCHC 1 cut(s) 433
Bsp143I GATC 2 cut(s) 259, 420
Bsp1720I GCTNAGC 1 cut(s) 339
Bsp68I TCGCGA 1 cut(s) 972
BspANI GGCC 3 cut(s) 284, 643, 789
BspCNI CTCAG 1 cut(s) 331
BspDI ATCGAT 1 cut(s) 122
BspFNI CGCG 1 cut(s) 972
BspLI GGNNCC 1 cut(s) 108
BsrDI GCAATG 1 cut(s) 821
BssECI CCNNGG 2 cut(s) 285, 791
BssMI GATC 2 cut(s) 259, 420
BssNAI GTATAC 1 cut(s) 702
BssT1I CCWWGG 1 cut(s) 791
Bst1107I GTATAC 1 cut(s) 702
Bst2UI CCWGG 2 cut(s) 640, 714
Bst4CI ACNGT 2 cut(s) 354, 1028
BstAPI GCANNNNNTGC 1 cut(s) 154
BstBAI YACGTR 1 cut(s) 428
BstC8I GCNNGC 2 cut(s) 230, 553
BstDEI CTNAG 4 cut(s) 339, 721, 1042, 1131
BstDSI CCRYGG 1 cut(s) 285
BstENI CCTNNNNNAGG 1 cut(s) 732
BstF5I GGATG 2 cut(s) 166, 697
BstFNI CGCG 1 cut(s) 972
BstH2I RGCGCY 1 cut(s) 346
BstHHI GCGC 2 cut(s) 324, 345
BstKTI GATC 2 cut(s) 262, 423
BstMBI GATC 2 cut(s) 259, 420
BstMWI GCNNNNNNNGC 1 cut(s) 154
BstNI CCWGG 2 cut(s) 640, 714
BstNSI RCATGY 5 cut(s) 209, 269, 555, 712, 933
BstSCI CCNGG 2 cut(s) 638, 712
BstUI CGCG 1 cut(s) 972
BstV1I GCAGC 2 cut(s) 322, 407
BstV2I GAAGAC 3 cut(s) 81, 913, 1107
BstZ17I GTATAC 1 cut(s) 702
Bsu15I ATCGAT 1 cut(s) 122
BsuI GTATCC 1 cut(s) 929
BsuRI GGCC 3 cut(s) 284, 643, 789
BsuTUI ATCGAT 1 cut(s) 122
BtgI CCRYGG 1 cut(s) 285
BtgZI GCGATG 1 cut(s) 856
BtsCI GGATG 2 cut(s) 166, 697
BtsI GCAGTG 1 cut(s) 639
BtsIMutI CAGTG 1 cut(s) 639
BtuMI TCGCGA 1 cut(s) 972
Cac8I GCNNGC 2 cut(s) 230, 553
CfoI GCGC 2 cut(s) 324, 345
Cfr13I GGNCC 3 cut(s) 523, 767, 788
ClaI ATCGAT 1 cut(s) 122
Csp6I GTAC 3 cut(s) 777, 854, 1029
CviAII CATG 9 cut(s) 196, 206, 266, 325, 544, 552, 565, 709, 930
CviJI RGCY 9 cut(s) 137, 284, 290, 335, 398, 643, 725, 789, 965
CviKI_1 RGCY 9 cut(s) 137, 284, 290, 335, 398, 643, 725, 789, 965
CviQI GTAC 3 cut(s) 777, 854, 1029
DdeI CTNAG 4 cut(s) 339, 721, 1042, 1131
DpnI GATC 2 cut(s) 261, 422
DpnII GATC 2 cut(s) 259, 420
DraI TTTAAA 2 cut(s) 243, 879
Eco130I CCWWGG 1 cut(s) 791
Eco47I GGWCC 2 cut(s) 523, 767
Eco47III AGCGCT 1 cut(s) 344
Eco57I CTGAAG 1 cut(s) 12
EcoNI CCTNNNNNAGG 1 cut(s) 732
EcoO109I RGGNCCY 3 cut(s) 523, 767, 788
EcoRII CCWGG 2 cut(s) 638, 712
EcoT14I CCWWGG 1 cut(s) 791
ErhI CCWWGG 1 cut(s) 791
FaeI CATG 9 cut(s) 199, 209, 269, 328, 547, 555, 568, 712, 933
FalI AAGNNNNNCTT 2 cut(s) 1047, 1079
FaqI GGGAC 1 cut(s) 613
FatI CATG 9 cut(s) 195, 205, 265, 324, 543, 551, 564, 708, 929
FauNDI CATATG 1 cut(s) 54
FblI GTMKAC 1 cut(s) 701
Fnu4HI GCNGC 2 cut(s) 336, 396
FokI GGATG 2 cut(s) 153, 704
Fsp4HI GCNGC 2 cut(s) 336, 396
FspBI CTAG 4 cut(s) 35, 588, 615, 792
GlaI GCGC 2 cut(s) 323, 344
GluI GCNGC 2 cut(s) 336, 396
GsaI CCCAGC 1 cut(s) 699
GsuI CTGGAG 2 cut(s) 153, 438
HaeII RGCGCY 1 cut(s) 346
HaeIII GGCC 3 cut(s) 284, 643, 789
HhaI GCGC 2 cut(s) 324, 345
Hin1II CATG 9 cut(s) 199, 209, 269, 328, 547, 555, 568, 712, 933
Hin6I GCGC 2 cut(s) 322, 343
HinP1I GCGC 2 cut(s) 322, 343
HinfI GANTC 3 cut(s) 14, 611, 717
HphI GGTGA 4 cut(s) 23, 565, 749, 1118
Hpy166II GTNNAC 5 cut(s) 573, 702, 779, 854, 1031
Hpy188I TCNGA 3 cut(s) 19, 31, 957
Hpy188III TCNNGA 6 cut(s) 132, 170, 278, 455, 493, 971
Hpy8I GTNNAC 5 cut(s) 573, 702, 779, 854, 1031
Hpy99I CGWCG 2 cut(s) 777, 972
HpyAV CCTTC 4 cut(s) 514, 590, 665, 952
HpyCH4III ACNGT 2 cut(s) 354, 1028
HpyCH4IV ACGT 3 cut(s) 427, 865, 916
HpyCH4V TGCA 8 cut(s) 148, 228, 551, 626, 632, 929, 1067, 1097
HpyF10VI GCNNNNNNNGC 1 cut(s) 154
HpyF3I CTNAG 4 cut(s) 339, 721, 1042, 1131
HpySE526I ACGT 3 cut(s) 427, 865, 916
Hsp92II CATG 9 cut(s) 199, 209, 269, 328, 547, 555, 568, 712, 933
HspAI GCGC 2 cut(s) 322, 343
Kzo9I GATC 2 cut(s) 259, 420
LmnI GCTCC 3 cut(s) 134, 560, 730
Lsp1109I GCAGC 2 cut(s) 322, 407
LweI GCATC 2 cut(s) 619, 682
MaeI CTAG 4 cut(s) 35, 588, 615, 792
MaeII ACGT 3 cut(s) 427, 865, 916
MaeIII GTNAC 4 cut(s) 11, 529, 634, 1050
MalI GATC 2 cut(s) 261, 422
MboI GATC 2 cut(s) 259, 420
MboII GAAGA 7 cut(s) 17, 18, 81, 810, 918, 1013, 1112
MhlI GDGCHC 1 cut(s) 433
MlyI GAGTC 1 cut(s) 8
MseI TTAA 4 cut(s) 242, 251, 381, 878
MslI CAYNNNNRTG 2 cut(s) 329, 753
MspR9I CCNGG 2 cut(s) 640, 714
Mva1269I GAATGC 1 cut(s) 273
MvaI CCWGG 2 cut(s) 640, 714
MvnI CGCG 1 cut(s) 972
MwoI GCNNNNNNNGC 1 cut(s) 154
NdeI CATATG 1 cut(s) 54
NdeII GATC 2 cut(s) 259, 420
NlaIII CATG 9 cut(s) 199, 209, 269, 328, 547, 555, 568, 712, 933
NlaIV GGNNCC 1 cut(s) 108
NmuCI GTSAC 3 cut(s) 11, 634, 1050
NruI TCGCGA 1 cut(s) 972
NspI RCATGY 5 cut(s) 209, 269, 555, 712, 933
PaeI GCATGC 1 cut(s) 555
PciI ACATGT 1 cut(s) 205
PctI GAATGC 1 cut(s) 273
PfeI GAWTC 2 cut(s) 611, 717
PflFI GACNNNGTC 1 cut(s) 771
PkrI GCNGC 2 cut(s) 337, 397
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
Ppu21I YACGTR 1 cut(s) 428
PpuMI RGGWCCY 2 cut(s) 523, 767
PscI ACATGT 1 cut(s) 205
PsiI TTATAA 1 cut(s) 183
Psp5II RGGWCCY 2 cut(s) 523, 767
Psp6I CCWGG 2 cut(s) 638, 712
PspFI CCCAGC 1 cut(s) 695
PspGI CCWGG 2 cut(s) 638, 712
PspN4I GGNNCC 1 cut(s) 108
PspPI GGNCC 3 cut(s) 523, 767, 788
PspPPI RGGWCCY 2 cut(s) 523, 767
PsyI GACNNNGTC 1 cut(s) 771
RruI TCGCGA 1 cut(s) 972
RsaI GTAC 3 cut(s) 778, 855, 1030
RsaNI GTAC 3 cut(s) 777, 854, 1029
RseI CAYNNNNRTG 2 cut(s) 329, 753
SaqAI TTAA 4 cut(s) 242, 251, 381, 878
SatI GCNGC 2 cut(s) 336, 396
Sau3AI GATC 2 cut(s) 259, 420
Sau96I GGNCC 3 cut(s) 523, 767, 788
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 2 cut(s) 640, 714
SduI GDGCHC 1 cut(s) 433
SfaNI GCATC 2 cut(s) 619, 682
SinI GGWCC 2 cut(s) 523, 767
SmiMI CAYNNNNRTG 2 cut(s) 329, 753
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
SphI GCATGC 1 cut(s) 555
SspMI CTAG 4 cut(s) 35, 588, 615, 792
StyD4I CCNGG 2 cut(s) 638, 712
StyI CCWWGG 1 cut(s) 791
TaaI ACNGT 2 cut(s) 354, 1028
TaiI ACGT 3 cut(s) 430, 868, 919
TaqI TCGA 4 cut(s) 122, 373, 492, 733
TatI WGTACW 2 cut(s) 853, 1028
TfiI GAWTC 2 cut(s) 611, 717
Tru1I TTAA 4 cut(s) 242, 251, 381, 878
Tru9I TTAA 4 cut(s) 242, 251, 381, 878
TscAI CASTG 1 cut(s) 639
TseFI GTSAC 3 cut(s) 11, 634, 1050
TseI GCWGC 2 cut(s) 335, 395
Tsp45I GTSAC 3 cut(s) 11, 634, 1050
TspDTI ATGAA 8 cut(s) 54, 81, 83, 359, 495, 684, 810, 1104
TspRI CASTG 1 cut(s) 639
Tth111I GACNNNGTC 1 cut(s) 771
VpaK11BI GGWCC 2 cut(s) 523, 767
XagI CCTNNNNNAGG 1 cut(s) 732
XapI RAATTY 4 cut(s) 177, 244, 459, 653
XceI RCATGY 5 cut(s) 209, 269, 555, 712, 933
XmaJI CCTAGG 1 cut(s) 791
XmiI GTMKAC 1 cut(s) 701
XspI CTAG 4 cut(s) 35, 588, 615, 792
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.