MD08G1193900.v1.1
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000469
Physical Location & Seq
Reverse (-)
24923562 .. 24926168
2607 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1193900.v1.1.491

Sequence Viewer

Length: 600 bp
ATGTTGGCATGTTCATTTAACATGATGTTCACATATGTCTATACCGGATGGGAAGGAACGGCTAATGACTCGAGAGTGTTGATGGATGCAATAACAAGAGAAGATAATATGTTTCCAATGCCTAAAGAAGGAAAATATTATGTTGTTGATTCCGGCTATGCCAACATGAGTGGGTTTCTTGCACCATATCGCAAAATGCGTTATCACTTGCGTGATTTTAGAGGAAGAGGTAGACGTCCAAGAGAAGCAATGGAATTGTTCAACTTTAGGCACTCATCACTACGTAATGTTGTTGAACGATGCATTGGAGTGCTTAAGAATTGTTTTCCTATTCTAAAGTTGATGCCAAATTATCCAATTAGGAAGCAAAGACGCATTCCCATTGCATGTTGTGCCGTGCACAATTTCATTAGAATGCAATCAAGGAATGATACTTTGTTTCAACAATTTGAAGACAATGATGTTGATGTGATAGATGAAGAAAGCTCGGGGACGAATCAAGAAGGGGAAAACATGCATTTGAATGACGACAATGTTATGAATGATGTTAGAGATCACATAGCCGGATTAATGTGGCTTGATTACGTCAATAACAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

200

Amino Acids

23.42

Weight (kDa)

6.52

Isoelectric Point (pI)

49.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDE_Tnp_4 PF13359 2 - 135 1.1e-17 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43722 AT3G30525 AT5G28730
fragaria_vesca FvH4_1g10461 FvH4_1g19401 FvH4_2g04530 FvH4_2g04530 FvH4_2g11121 FvH4_2g13361 FvH4_3g21471 FvH4_3g26161 FvH4_3g29911 FvH4_4g20922 FvH4_4g30624 FvH4_5g23912 FvH4_6g30381 FvH4_6g53026 FvH4_7g00061 FvH4_7g19211 FvH4_7g19211
malus_domestica MD06G1090500.v1.1 MD08G1193900.v1.1 MD11G1151600.v1.1 MD16G1281600.v1.1
prunus_persica Prupe.1G096200_v2.0.a1 Prupe.1G189200_v2.0.a1 Prupe.1G198400_v2.0.a1 Prupe.6G167200_v2.0.a1 Prupe.6G192900_v2.0.a1
pyrus_communis pycom02g00900 pycom06g03500 pycom07g13740 pycom11g10040 pycom17g21680 pycom520g00090
rosa_chinensis RchiOBHm_Chr1g0354781 RchiOBHm_Chr2g0085491 RchiOBHm_Chr2g0103971 RchiOBHm_Chr2g0145791 RchiOBHm_Chr4g0414051 RchiOBHm_Chr4g0419691 RchiOBHm_Chr4g0435871 RchiOBHm_Chr6g0292381 RchiOBHm_Chr7g0199291
rosa_laevigata RLG00000035604
rosa_multiflora Rmu_co8287949.1_g000001 Rmu_sc0000079.1_g000057 Rmu_sc0000240.1_g000063 Rmu_sc0000552.1_g000020 Rmu_sc0000950.1_g000002 Rmu_sc0001257.1_g000003 Rmu_sc0001597.1_g000014 Rmu_sc0001939.1_g000006 Rmu_sc0002160.1_g000015 Rmu_sc0003526.1_g000015 Rmu_sc0005712.1_g000025 Rmu_sc0006032.1_g000011 Rmu_sc0006935.1_g000005 Rmu_sc0008636.1_g000002 Rmu_sc0009205.1_g000006 Rmu_sc0009438.1_g000007 Rmu_ssc0000486.1_g000013
rosa_roxburghii Rroxscaffold_1G00003870 Rroxscaffold_1G00014970 Rroxscaffold_3G00218750 Rroxscaffold_5G00367160 Rroxscaffold_7G00186480
rosa_rugosa Rorug05G0192000
rosa_samantha Rh4BG219200
rosa_wichuraiana Rw1G010350 Rw1G014480 Rw1G023690 Rw1G033480 Rw2G050290 Rw3G027480 Rw4G033650 Rw5G005670 Rw5G021010 Rw5G021860 Rw5G041260 Rw5G041760 Rw6G008410 Rw6G021080 Rw6G022950 Rw6G025480 Rw6G028700 Rw7G002710 Rw7G004940 Rw7G031510 Rw7G041110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 238
AccI GTMKAC 1 cut(s) 232
AcyI GRCGYC 1 cut(s) 235
AfiI CCNNNNNNNGG 1 cut(s) 128
AflII CTTAAG 1 cut(s) 314
AgsI TTSAA 5 cut(s) 262, 296, 443, 452, 523
AjuI GAANNNNNNNTTGG 2 cut(s) 288, 320
AleI CACNNNNGTG 1 cut(s) 210
AluBI AGCT 1 cut(s) 486
AluI AGCT 1 cut(s) 486
Alw21I GWGCWC 1 cut(s) 402
Alw44I GTGCAC 1 cut(s) 398
Ama87I CYCGRG 2 cut(s) 70, 487
ApaLI GTGCAC 1 cut(s) 398
AseI ATTAAT 1 cut(s) 569
AvaI CYCGRG 2 cut(s) 70, 487
BaeGI GKGCMC 1 cut(s) 402
BbsI GAAGAC 1 cut(s) 459
Bbv12I GWGCWC 1 cut(s) 402
BccI CCATC 2 cut(s) 42, 76
BceAI ACGGC 2 cut(s) 75, 380
BfrI CTTAAG 1 cut(s) 314
BmeT110I CYCGRG 2 cut(s) 70, 487
BmsI GCATC 3 cut(s) 76, 290, 333
BpiI GAAGAC 1 cut(s) 459
BsaAI YACGTR 1 cut(s) 284
BsaHI GRCGYC 1 cut(s) 235
BsaWI WCCGGW 1 cut(s) 44
Bsc4I CCNNNNNNNGG 1 cut(s) 128
Bse3DI GCAATG 2 cut(s) 255, 381
BseGI GGATG 2 cut(s) 53, 91
BseLI CCNNNNNNNGG 1 cut(s) 128
BseMI GCAATG 2 cut(s) 255, 381
BseSI GKGCMC 1 cut(s) 402
BsiHKAI GWGCWC 1 cut(s) 402
BsiHKCI CYCGRG 2 cut(s) 70, 487
BsiSI CCGG 3 cut(s) 45, 153, 564
BslFI GGGAC 1 cut(s) 505
BslI CCNNNNNNNGG 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 505
BsmI GAATGC 2 cut(s) 375, 420
BsoBI CYCGRG 2 cut(s) 70, 487
Bsp1286I GDGCHC 1 cut(s) 402
Bsp143I GATC 1 cut(s) 553
BspTI CTTAAG 1 cut(s) 314
BsrDI GCAATG 2 cut(s) 255, 381
BssMI GATC 1 cut(s) 553
BssNI GRCGYC 1 cut(s) 235
Bst6I CTCTTC 1 cut(s) 220
BstACI GRCGYC 1 cut(s) 235
BstAFI CTTAAG 1 cut(s) 314
BstAPI GCANNNNNTGC 1 cut(s) 392
BstBAI YACGTR 1 cut(s) 284
BstENI CCTNNNNNAGG 1 cut(s) 126
BstF5I GGATG 2 cut(s) 53, 91
BstKTI GATC 1 cut(s) 556
BstMBI GATC 1 cut(s) 553
BstMWI GCNNNNNNNGC 1 cut(s) 392
BstNSI RCATGY 3 cut(s) 12, 390, 517
BstSLI GKGCMC 1 cut(s) 402
BstSNI TACGTA 1 cut(s) 284
BstV2I GAAGAC 1 cut(s) 459
BtsCI GGATG 2 cut(s) 53, 91
CseI GACGC 1 cut(s) 381
CspCI CAANNNNNGTGG 2 cut(s) 151, 186
CviAII CATG 5 cut(s) 9, 22, 166, 387, 514
CviJI RGCY 5 cut(s) 62, 156, 486, 563, 577
CviKI_1 RGCY 5 cut(s) 62, 156, 486, 563, 577
DpnI GATC 1 cut(s) 555
DpnII GATC 1 cut(s) 553
Eam1104I CTCTTC 1 cut(s) 220
EarI CTCTTC 1 cut(s) 220
Eco105I TACGTA 1 cut(s) 284
Eco88I CYCGRG 2 cut(s) 70, 487
EcoNI CCTNNNNNAGG 1 cut(s) 126
EcoT22I ATGCAT 2 cut(s) 305, 519
FaeI CATG 5 cut(s) 12, 25, 169, 390, 517
FaqI GGGAC 1 cut(s) 505
FatI CATG 5 cut(s) 8, 21, 165, 386, 513
FauNDI CATATG 1 cut(s) 34
FblI GTMKAC 1 cut(s) 232
FokI GGATG 2 cut(s) 60, 98
HapII CCGG 3 cut(s) 45, 153, 564
HgaI GACGC 1 cut(s) 381
Hin1I GRCGYC 1 cut(s) 235
Hin1II CATG 5 cut(s) 12, 25, 169, 390, 517
HinfI GANTC 3 cut(s) 68, 149, 496
HpaII CCGG 3 cut(s) 45, 153, 564
Hpy166II GTNNAC 3 cut(s) 30, 233, 400
Hpy188III TCNNGA 2 cut(s) 72, 500
Hpy8I GTNNAC 3 cut(s) 30, 233, 400
HpyAV CCTTC 3 cut(s) 47, 122, 497
HpyCH4IV ACGT 3 cut(s) 235, 283, 585
HpyCH4V TGCA 7 cut(s) 89, 182, 303, 386, 400, 418, 517
HpyF10VI GCNNNNNNNGC 1 cut(s) 392
HpySE526I ACGT 3 cut(s) 235, 283, 585
Hsp92I GRCGYC 1 cut(s) 235
Hsp92II CATG 5 cut(s) 12, 25, 169, 390, 517
Kzo9I GATC 1 cut(s) 553
LpnPI CCDG 3 cut(s) 58, 166, 577
LweI GCATC 3 cut(s) 76, 290, 333
MaeII ACGT 3 cut(s) 235, 283, 585
MalI GATC 1 cut(s) 555
MboI GATC 1 cut(s) 553
MboII GAAGA 4 cut(s) 113, 237, 464, 491
MhlI GDGCHC 1 cut(s) 402
MluCI AATT 7 cut(s) 254, 319, 349, 357, 403, 446, 595
MlyI GAGTC 1 cut(s) 62
MnlI CCTC 2 cut(s) 215, 221
Mph1103I ATGCAT 2 cut(s) 305, 519
MseI TTAA 3 cut(s) 18, 315, 569
MslI CAYNNNNRTG 4 cut(s) 210, 308, 413, 522
MspCI CTTAAG 1 cut(s) 314
MspI CCGG 3 cut(s) 45, 153, 564
Mva1269I GAATGC 2 cut(s) 375, 420
MwoI GCNNNNNNNGC 1 cut(s) 392
NdeI CATATG 1 cut(s) 34
NdeII GATC 1 cut(s) 553
NlaIII CATG 5 cut(s) 12, 25, 169, 390, 517
NsiI ATGCAT 2 cut(s) 305, 519
NspI RCATGY 3 cut(s) 12, 390, 517
OliI CACNNNNGTG 1 cut(s) 210
PaeR7I CTCGAG 1 cut(s) 70
PcsI WCGNNNNNNNCGW 1 cut(s) 196
PctI GAATGC 2 cut(s) 375, 420
PfeI GAWTC 2 cut(s) 149, 496
PleI GAGTC 1 cut(s) 62
PpsI GAGTC 1 cut(s) 62
Ppu21I YACGTR 1 cut(s) 284
PshBI ATTAAT 1 cut(s) 569
RseI CAYNNNNRTG 4 cut(s) 210, 308, 413, 522
SaqAI TTAA 3 cut(s) 18, 315, 569
Sau3AI GATC 1 cut(s) 553
SchI GAGTC 1 cut(s) 62
SduI GDGCHC 1 cut(s) 402
SetI ASST 5 cut(s) 232, 238, 286, 488, 588
SfaNI GCATC 3 cut(s) 76, 290, 333
Sfr274I CTCGAG 1 cut(s) 70
SlaI CTCGAG 1 cut(s) 70
SmiMI CAYNNNNRTG 4 cut(s) 210, 308, 413, 522
SmlI CTYRAG 2 cut(s) 70, 314
SmoI CTYRAG 2 cut(s) 70, 314
SnaBI TACGTA 1 cut(s) 284
Sse9I AATT 7 cut(s) 254, 319, 349, 357, 403, 446, 595
SspI AATATT 1 cut(s) 137
TaiI ACGT 3 cut(s) 238, 286, 588
TaqI TCGA 1 cut(s) 71
TasI AATT 7 cut(s) 254, 319, 349, 357, 403, 446, 595
TfiI GAWTC 2 cut(s) 149, 496
Tru1I TTAA 3 cut(s) 18, 315, 569
Tru9I TTAA 3 cut(s) 18, 315, 569
TspDTI ATGAA 3 cut(s) 397, 492, 554
Vha464I CTTAAG 1 cut(s) 314
VneI GTGCAC 1 cut(s) 398
VspI ATTAAT 1 cut(s) 569
XagI CCTNNNNNAGG 1 cut(s) 126
XceI RCATGY 3 cut(s) 12, 390, 517
XhoI CTCGAG 1 cut(s) 70
XmiI GTMKAC 1 cut(s) 232
ZraI GACGTC 1 cut(s) 236
Zsp2I ATGCAT 2 cut(s) 305, 519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.