Rw7G002710
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0000469
Physical Location & Seq
Forward (+)
2231550 .. 2232436
887 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G002710.1

Sequence Viewer

Length: 714 bp
ATGGATGCGTCTGGAGATGAGGAGGAGATAGATCAAATTATATGTTTATGTGGTCGTCTTATTCAAGTCTATCTTGAAAAGTACATTCAGAAAACTCCATGTATGACAAGCTCTCAAACAGGTTACATTTGGTTAATGGAAGTGTTAGAAGGCAATGAAAGTAGGTGTTATAATCAATTTAGGATGGATAAACAAGTCTTCATGATGCTTGTAAATGATTTGGAAAAGATAATGAAGGGTTCAAGAAACATGAGTATCGTTGAAATGTTGGCTATGTTTCTTTATATTTTGGGACAAGGTCAGACTAATAGGAGTACACAAGAACGTTTTCAACATTCTGGTGAGACGGTTAGTAGGTATTTTGATATAATGTTAGAGATTTTCTATAATATGGCAAAAGTGCTCATAAATCCAAGGGATCCGGAATTTAGGAGCATTCCACAAGAAATTCTCACTGATTCTAGATACATGCCTCATTTTAAGGTATGTATAATAAAATGGATACCCACCCAAAATGTATTGGCTATATGCAACTTCGACATGCAATTCACATTTGCATGTGCAGGGTGGGAAGGCACCGCCCATGATAGTAGAGTATTCTTATCAGCTCTACGCAATCCTCAATCGAATTTTCCTAAACCCCCAAATGGTATTTTTTTTCCGAGAATTACACAAATAATAACTTATAATCTTTTATTTTACCGAGATCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

237

Amino Acids

27.82

Weight (kDa)

5.69

Isoelectric Point (pI)

40.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 36 - 128 9.7e-35 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43722 AT3G30525 AT5G28730
fragaria_vesca FvH4_1g10461 FvH4_1g19401 FvH4_2g04530 FvH4_2g04530 FvH4_2g11121 FvH4_2g13361 FvH4_3g21471 FvH4_3g26161 FvH4_3g29911 FvH4_4g20922 FvH4_4g30624 FvH4_5g23912 FvH4_6g30381 FvH4_6g53026 FvH4_7g00061 FvH4_7g19211 FvH4_7g19211
malus_domestica MD06G1090500.v1.1 MD08G1193900.v1.1 MD11G1151600.v1.1 MD16G1281600.v1.1
prunus_persica Prupe.1G096200_v2.0.a1 Prupe.1G189200_v2.0.a1 Prupe.1G198400_v2.0.a1 Prupe.6G167200_v2.0.a1 Prupe.6G192900_v2.0.a1
pyrus_communis pycom02g00900 pycom06g03500 pycom07g13740 pycom11g10040 pycom17g21680 pycom520g00090
rosa_chinensis RchiOBHm_Chr1g0354781 RchiOBHm_Chr2g0085491 RchiOBHm_Chr2g0103971 RchiOBHm_Chr2g0145791 RchiOBHm_Chr4g0414051 RchiOBHm_Chr4g0419691 RchiOBHm_Chr4g0435871 RchiOBHm_Chr6g0292381 RchiOBHm_Chr7g0199291
rosa_laevigata RLG00000035604
rosa_multiflora Rmu_co8287949.1_g000001 Rmu_sc0000079.1_g000057 Rmu_sc0000240.1_g000063 Rmu_sc0000552.1_g000020 Rmu_sc0000950.1_g000002 Rmu_sc0001257.1_g000003 Rmu_sc0001597.1_g000014 Rmu_sc0001939.1_g000006 Rmu_sc0002160.1_g000015 Rmu_sc0003526.1_g000015 Rmu_sc0005712.1_g000025 Rmu_sc0006032.1_g000011 Rmu_sc0006935.1_g000005 Rmu_sc0008636.1_g000002 Rmu_sc0009205.1_g000006 Rmu_sc0009438.1_g000007 Rmu_ssc0000486.1_g000013
rosa_roxburghii Rroxscaffold_1G00003870 Rroxscaffold_1G00014970 Rroxscaffold_3G00218750 Rroxscaffold_5G00367160 Rroxscaffold_7G00186480
rosa_rugosa Rorug05G0192000
rosa_samantha Rh4BG219200
rosa_wichuraiana Rw1G010350 Rw1G014480 Rw1G023690 Rw1G033480 Rw2G050290 Rw3G027480 Rw4G033650 Rw5G005670 Rw5G021010 Rw5G021860 Rw5G041260 Rw5G041760 Rw6G008410 Rw6G021080 Rw6G022950 Rw6G025480 Rw6G028700 Rw7G002710 Rw7G004940 Rw7G031510 Rw7G041110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 171, 687
AccB1I GGYRCC 1 cut(s) 575
AccIII TCCGGA 1 cut(s) 421
AciI CCGC 1 cut(s) 579
AclI AACGTT 1 cut(s) 325
AclWI GGATC 2 cut(s) 413, 426
AcsI RAATTY 3 cut(s) 425, 447, 628
AfaI GTAC 2 cut(s) 83, 316
AfiI CCNNNNNNNGG 1 cut(s) 647
AgsI TTSAA 5 cut(s) 65, 77, 243, 263, 332
AluBI AGCT 2 cut(s) 111, 608
AluI AGCT 2 cut(s) 111, 608
Alw21I GWGCWC 1 cut(s) 405
Alw26I GTCTC 1 cut(s) 338
AlwI GGATC 2 cut(s) 413, 426
Aor13HI TCCGGA 1 cut(s) 421
ApoI RAATTY 3 cut(s) 425, 447, 628
Asp700I GAANNNNTTC 1 cut(s) 327
AsuHPI GGTGA 1 cut(s) 353
BaeI ACNNNNGTAYC 2 cut(s) 238, 271
BamHI GGATCC 1 cut(s) 418
BanI GGYRCC 1 cut(s) 575
BbsI GAAGAC 1 cut(s) 190
Bbv12I GWGCWC 1 cut(s) 405
BccI CCATC 1 cut(s) 178
BciVI GTATCC 1 cut(s) 495
BcoDI GTCTC 1 cut(s) 338
BfaI CTAG 1 cut(s) 462
BfuI GTATCC 1 cut(s) 495
BmiI GGNNCC 2 cut(s) 420, 577
BmsI GCATC 1 cut(s) 195
BpiI GAAGAC 1 cut(s) 190
BpmI CTGGAG 1 cut(s) 33
BsaJI CCNNGG 1 cut(s) 413
BsaWI WCCGGW 1 cut(s) 421
Bsc4I CCNNNNNNNGG 1 cut(s) 647
Bse3DI GCAATG 1 cut(s) 160
BseAI TCCGGA 1 cut(s) 421
BseDI CCNNGG 1 cut(s) 413
BseGI GGATG 2 cut(s) 10, 189
BseLI CCNNNNNNNGG 1 cut(s) 647
BseMI GCAATG 1 cut(s) 160
BseRI GAGGAG 2 cut(s) 35, 38
BsgI GTGCAG 1 cut(s) 582
BshNI GGYRCC 1 cut(s) 575
BsiHKAI GWGCWC 1 cut(s) 405
BsiSI CCGG 1 cut(s) 422
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 1 cut(s) 647
BsmAI GTCTC 1 cut(s) 338
BsmBI CGTCTC 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 306
BsmI GAATGC 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 405
Bsp13I TCCGGA 1 cut(s) 421
Bsp143I GATC 3 cut(s) 31, 418, 706
BspACI CCGC 1 cut(s) 579
BspEI TCCGGA 1 cut(s) 421
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 2 cut(s) 420, 577
BspPI GGATC 2 cut(s) 413, 426
BspT107I GGYRCC 1 cut(s) 575
BsrDI GCAATG 1 cut(s) 160
BssECI CCNNGG 1 cut(s) 413
BssMI GATC 3 cut(s) 31, 418, 706
BssT1I CCWWGG 1 cut(s) 413
Bst4CI ACNGT 1 cut(s) 349
BstF5I GGATG 2 cut(s) 10, 189
BstKTI GATC 3 cut(s) 34, 421, 709
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 3 cut(s) 31, 418, 706
BstNSI RCATGY 3 cut(s) 472, 544, 561
BstV2I GAAGAC 1 cut(s) 190
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BsuI GTATCC 1 cut(s) 495
BtsCI GGATG 2 cut(s) 10, 189
BtsIMutI CAGTG 1 cut(s) 453
CciI TCATGA 1 cut(s) 201
Csp6I GTAC 2 cut(s) 82, 315
CviAII CATG 7 cut(s) 99, 202, 250, 469, 541, 558, 584
CviJI RGCY 4 cut(s) 111, 272, 524, 608
CviKI_1 RGCY 4 cut(s) 111, 272, 524, 608
CviQI GTAC 2 cut(s) 82, 315
DpnI GATC 3 cut(s) 33, 420, 708
DpnII GATC 3 cut(s) 31, 418, 706
Eco130I CCWWGG 1 cut(s) 413
EcoT14I CCWWGG 1 cut(s) 413
ErhI CCWWGG 1 cut(s) 413
Esp3I CGTCTC 1 cut(s) 338
FaeI CATG 7 cut(s) 102, 205, 253, 472, 544, 561, 587
FalI AAGNNNNNCTT 2 cut(s) 57, 89
FaqI GGGAC 1 cut(s) 306
FatI CATG 7 cut(s) 98, 201, 249, 468, 540, 557, 583
FokI GGATG 2 cut(s) 17, 196
FspBI CTAG 1 cut(s) 462
GsuI CTGGAG 1 cut(s) 33
HapII CCGG 1 cut(s) 422
Hin1II CATG 7 cut(s) 102, 205, 253, 472, 544, 561, 587
HinfI GANTC 1 cut(s) 458
HpaII CCGG 1 cut(s) 422
HphI GGTGA 1 cut(s) 353
Hpy166II GTNNAC 1 cut(s) 317
Hpy188I TCNGA 3 cut(s) 90, 303, 663
Hpy188III TCNNGA 6 cut(s) 12, 74, 202, 243, 422, 462
Hpy8I GTNNAC 1 cut(s) 317
HpyAV CCTTC 3 cut(s) 143, 229, 566
HpyCH4III ACNGT 1 cut(s) 349
HpyCH4IV ACGT 1 cut(s) 325
HpyCH4V TGCA 4 cut(s) 531, 544, 557, 563
HpySE526I ACGT 1 cut(s) 325
Hsp92II CATG 7 cut(s) 102, 205, 253, 472, 544, 561, 587
Kpn2I TCCGGA 1 cut(s) 421
Kzo9I GATC 3 cut(s) 31, 418, 706
LmnI GCTCC 1 cut(s) 432
LpnPI CCDG 4 cut(s) 105, 324, 435, 549
LweI GCATC 1 cut(s) 195
MaeI CTAG 1 cut(s) 462
MaeII ACGT 1 cut(s) 325
MaeIII GTNAC 1 cut(s) 122
MalI GATC 3 cut(s) 33, 420, 708
MboI GATC 3 cut(s) 31, 418, 706
MboII GAAGA 1 cut(s) 190
MflI RGATCY 1 cut(s) 418
MhlI GDGCHC 1 cut(s) 405
MluCI AATT 7 cut(s) 36, 176, 425, 447, 545, 628, 666
MnlI CCTC 4 cut(s) 13, 16, 483, 630
MroI TCCGGA 1 cut(s) 421
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 2 cut(s) 134, 480
MslI CAYNNNNRTG 2 cut(s) 339, 556
MspI CCGG 1 cut(s) 422
Mva1269I GAATGC 1 cut(s) 435
NdeII GATC 3 cut(s) 31, 418, 706
NlaIII CATG 7 cut(s) 102, 205, 253, 472, 544, 561, 587
NlaIV GGNNCC 2 cut(s) 420, 577
NspI RCATGY 3 cut(s) 472, 544, 561
PagI TCATGA 1 cut(s) 201
PctI GAATGC 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 327
PfeI GAWTC 1 cut(s) 458
PflFI GACNNNGTC 1 cut(s) 297
PsiI TTATAA 2 cut(s) 171, 687
Psp1406I AACGTT 1 cut(s) 325
PspN4I GGNNCC 2 cut(s) 420, 577
PsuI RGATCY 1 cut(s) 418
PsyI GACNNNGTC 1 cut(s) 297
RsaI GTAC 2 cut(s) 83, 316
RsaNI GTAC 2 cut(s) 82, 315
RseI CAYNNNNRTG 2 cut(s) 339, 556
SaqAI TTAA 2 cut(s) 134, 480
Sau3AI GATC 3 cut(s) 31, 418, 706
SduI GDGCHC 1 cut(s) 405
SetI ASST 8 cut(s) 113, 124, 167, 301, 328, 359, 486, 610
SfaNI GCATC 1 cut(s) 195
SmiMI CAYNNNNRTG 2 cut(s) 339, 556
Sse9I AATT 7 cut(s) 36, 176, 425, 447, 545, 628, 666
SsiI CCGC 1 cut(s) 579
SspMI CTAG 1 cut(s) 462
StyI CCWWGG 1 cut(s) 413
TaaI ACNGT 1 cut(s) 349
TaiI ACGT 1 cut(s) 328
TaqI TCGA 2 cut(s) 537, 626
TasI AATT 7 cut(s) 36, 176, 425, 447, 545, 628, 666
TatI WGTACW 2 cut(s) 81, 314
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 2 cut(s) 134, 480
Tru9I TTAA 2 cut(s) 134, 480
TscAI CASTG 1 cut(s) 460
TspDTI ATGAA 3 cut(s) 171, 190, 248
TspRI CASTG 1 cut(s) 460
Tth111I GACNNNGTC 1 cut(s) 297
XapI RAATTY 3 cut(s) 425, 447, 628
XbaI TCTAGA 1 cut(s) 461
XceI RCATGY 3 cut(s) 472, 544, 561
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.