Rw1G023690
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000469
Physical Location & Seq
Forward (+)
49659285 .. 49660104
820 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G023690.1

Sequence Viewer

Length: 660 bp
ATGGACACTTCCGGAGATGAGGAGGAGATAGACCAAATTATATGTGTATGTGGTCGTCTCATCCAAGTCTATCTCGAAAAGTACATTATGAAAACGCCATGCATGACAAGCTCTCAAACAGGTTATATTTGGTTAATGGAAGTGTTTGAAGGCAATGAAAGTCGGTGTTATAACCAATTTAGGATGGATAAGTACTGCTTCTTAATGCTATTGAATGATGTTGAAAAGGTGTTGAAGGGTTCAAAAAACATAAGTACCGCTGAAATGTTAGCTATGTTTCTCTATATTTTGGGACAAAGTCAAACCAATAGGAATACACAAGAACGTTTTCAACGTTCCGGTGAGGCGGTTAGTAGATATTTTGATATAATGCTCAACATTTTCTATAATATGGCGAAAGTACTCATAAAGCCATCGGATCCCGAATTTACGAACATCTCAAATGAAATTCTAAATGACTCAAGATACATGCCTCATTTTAAGGTATGTATAAGAACACCGACCCAGAATATTTTGGCTATATGCGACTTCAACATGCAATTCACATTTGCATGTGCAGGATGGGAAGGCACTGCACATGATAGTAGAGTATTCTTATCGGCTTTTCGTAATCCTCGATCGAATTTTCCTAAACCCCCAAATGGTACATTTTATACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

219

Amino Acids

25.48

Weight (kDa)

5.77

Isoelectric Point (pI)

43.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 36 - 128 1.4e-30 Domain of unknown function (DUF8040)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43722 AT3G30525 AT5G28730
fragaria_vesca FvH4_1g10461 FvH4_1g19401 FvH4_2g04530 FvH4_2g04530 FvH4_2g11121 FvH4_2g13361 FvH4_3g21471 FvH4_3g26161 FvH4_3g29911 FvH4_4g20922 FvH4_4g30624 FvH4_5g23912 FvH4_6g30381 FvH4_6g53026 FvH4_7g00061 FvH4_7g19211 FvH4_7g19211
malus_domestica MD06G1090500.v1.1 MD08G1193900.v1.1 MD11G1151600.v1.1 MD16G1281600.v1.1
prunus_persica Prupe.1G096200_v2.0.a1 Prupe.1G189200_v2.0.a1 Prupe.1G198400_v2.0.a1 Prupe.6G167200_v2.0.a1 Prupe.6G192900_v2.0.a1
pyrus_communis pycom02g00900 pycom06g03500 pycom07g13740 pycom11g10040 pycom17g21680 pycom520g00090
rosa_chinensis RchiOBHm_Chr1g0354781 RchiOBHm_Chr2g0085491 RchiOBHm_Chr2g0103971 RchiOBHm_Chr2g0145791 RchiOBHm_Chr4g0414051 RchiOBHm_Chr4g0419691 RchiOBHm_Chr4g0435871 RchiOBHm_Chr6g0292381 RchiOBHm_Chr7g0199291
rosa_laevigata RLG00000035604
rosa_multiflora Rmu_co8287949.1_g000001 Rmu_sc0000079.1_g000057 Rmu_sc0000240.1_g000063 Rmu_sc0000552.1_g000020 Rmu_sc0000950.1_g000002 Rmu_sc0001257.1_g000003 Rmu_sc0001597.1_g000014 Rmu_sc0001939.1_g000006 Rmu_sc0002160.1_g000015 Rmu_sc0003526.1_g000015 Rmu_sc0005712.1_g000025 Rmu_sc0006032.1_g000011 Rmu_sc0006935.1_g000005 Rmu_sc0008636.1_g000002 Rmu_sc0009205.1_g000006 Rmu_sc0009438.1_g000007 Rmu_ssc0000486.1_g000013
rosa_roxburghii Rroxscaffold_1G00003870 Rroxscaffold_1G00014970 Rroxscaffold_3G00218750 Rroxscaffold_5G00367160 Rroxscaffold_7G00186480
rosa_rugosa Rorug05G0192000
rosa_samantha Rh4BG219200
rosa_wichuraiana Rw1G010350 Rw1G014480 Rw1G023690 Rw1G033480 Rw2G050290 Rw3G027480 Rw4G033650 Rw5G005670 Rw5G021010 Rw5G021860 Rw5G041260 Rw5G041760 Rw6G008410 Rw6G021080 Rw6G022950 Rw6G025480 Rw6G028700 Rw7G002710 Rw7G004940 Rw7G031510 Rw7G041110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 171
AccIII TCCGGA 1 cut(s) 11
AciI CCGC 2 cut(s) 258, 347
AclI AACGTT 2 cut(s) 325, 334
AclWI GGATC 2 cut(s) 413, 426
AcsI RAATTY 3 cut(s) 425, 447, 622
AfaI GTAC 5 cut(s) 83, 194, 256, 402, 646
AfiI CCNNNNNNNGG 1 cut(s) 641
AgsI TTSAA 7 cut(s) 149, 214, 224, 235, 243, 332, 532
AluBI AGCT 2 cut(s) 111, 272
AluI AGCT 2 cut(s) 111, 272
Alw26I GTCTC 1 cut(s) 62
AlwI GGATC 2 cut(s) 413, 426
Aor13HI TCCGGA 1 cut(s) 11
ApoI RAATTY 3 cut(s) 425, 447, 622
Asp700I GAANNNNTTC 1 cut(s) 327
AsuHPI GGTGA 1 cut(s) 353
BaeI ACNNNNGTAYC 2 cut(s) 238, 271
BamHI GGATCC 1 cut(s) 418
BccI CCATC 3 cut(s) 178, 421, 555
BcoDI GTCTC 1 cut(s) 62
BfaI CTAG 1 cut(s) 658
BmcAI AGTACT 2 cut(s) 194, 402
BmiI GGNNCC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 445
BsaWI WCCGGW 2 cut(s) 11, 338
Bsc4I CCNNNNNNNGG 1 cut(s) 641
Bse3DI GCAATG 1 cut(s) 160
BseAI TCCGGA 1 cut(s) 11
BseGI GGATG 3 cut(s) 60, 189, 566
BseLI CCNNNNNNNGG 1 cut(s) 641
BseMI GCAATG 1 cut(s) 160
BseRI GAGGAG 2 cut(s) 35, 38
BsgI GTGCAG 2 cut(s) 558, 576
Bsh1285I CGRYCG 1 cut(s) 620
BsiEI CGRYCG 1 cut(s) 620
BsiSI CCGG 2 cut(s) 12, 339
BslFI GGGAC 1 cut(s) 306
BslI CCNNNNNNNGG 1 cut(s) 641
BsmAI GTCTC 1 cut(s) 62
BsmBI CGTCTC 1 cut(s) 62
BsmFI GGGAC 1 cut(s) 306
Bsp13I TCCGGA 1 cut(s) 11
Bsp143I GATC 2 cut(s) 418, 617
BspACI CCGC 2 cut(s) 258, 347
BspEI TCCGGA 1 cut(s) 11
BspLI GGNNCC 1 cut(s) 420
BspPI GGATC 2 cut(s) 413, 426
BsrDI GCAATG 1 cut(s) 160
BssMI GATC 2 cut(s) 418, 617
BstF5I GGATG 3 cut(s) 60, 189, 566
BstKTI GATC 2 cut(s) 421, 620
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 2 cut(s) 418, 617
BstMCI CGRYCG 1 cut(s) 620
BstMWI GCNNNNNNNGC 1 cut(s) 108
BstNSI RCATGY 3 cut(s) 472, 538, 555
BstX2I RGATCY 1 cut(s) 418
BstYI RGATCY 1 cut(s) 418
BtsCI GGATG 3 cut(s) 60, 189, 566
BtsI GCAGTG 1 cut(s) 570
BtsIMutI CAGTG 1 cut(s) 570
Csp6I GTAC 5 cut(s) 82, 193, 255, 401, 645
CviAII CATG 6 cut(s) 99, 103, 469, 535, 552, 578
CviJI RGCY 5 cut(s) 111, 272, 412, 518, 602
CviKI_1 RGCY 5 cut(s) 111, 272, 412, 518, 602
CviQI GTAC 5 cut(s) 82, 193, 255, 401, 645
DpnI GATC 2 cut(s) 420, 619
DpnII GATC 2 cut(s) 418, 617
EcoT22I ATGCAT 1 cut(s) 104
Esp3I CGTCTC 1 cut(s) 62
FaeI CATG 6 cut(s) 102, 106, 472, 538, 555, 581
FalI AAGNNNNNCTT 2 cut(s) 182, 214
FaqI GGGAC 1 cut(s) 306
FatI CATG 6 cut(s) 98, 102, 468, 534, 551, 577
FokI GGATG 3 cut(s) 47, 196, 573
FspBI CTAG 1 cut(s) 658
HapII CCGG 2 cut(s) 12, 339
Hin1II CATG 6 cut(s) 102, 106, 472, 538, 555, 581
HinfI GANTC 1 cut(s) 458
HpaII CCGG 2 cut(s) 12, 339
HphI GGTGA 1 cut(s) 353
Hpy188I TCNGA 1 cut(s) 418
Hpy188III TCNNGA 4 cut(s) 12, 74, 422, 462
HpyAV CCTTC 3 cut(s) 143, 229, 560
HpyCH4IV ACGT 2 cut(s) 325, 334
HpyCH4V TGCA 5 cut(s) 102, 538, 551, 557, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 108
HpySE526I ACGT 2 cut(s) 325, 334
Hsp92II CATG 6 cut(s) 102, 106, 472, 538, 555, 581
Kpn2I TCCGGA 1 cut(s) 11
Kzo9I GATC 2 cut(s) 418, 617
LpnPI CCDG 5 cut(s) 25, 105, 352, 518, 543
MaeI CTAG 1 cut(s) 658
MaeII ACGT 2 cut(s) 325, 334
MalI GATC 2 cut(s) 420, 619
MboI GATC 2 cut(s) 418, 617
MflI RGATCY 1 cut(s) 418
MluCI AATT 6 cut(s) 36, 176, 425, 447, 539, 622
MlyI GAGTC 1 cut(s) 452
MnlI CCTC 5 cut(s) 13, 16, 337, 483, 624
Mph1103I ATGCAT 1 cut(s) 104
MroI TCCGGA 1 cut(s) 11
MroXI GAANNNNTTC 1 cut(s) 327
MseI TTAA 3 cut(s) 134, 203, 480
MslI CAYNNNNRTG 1 cut(s) 550
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 2 cut(s) 12, 339
MwoI GCNNNNNNNGC 1 cut(s) 108
NdeII GATC 2 cut(s) 418, 617
NlaIII CATG 6 cut(s) 102, 106, 472, 538, 555, 581
NlaIV GGNNCC 1 cut(s) 420
NsiI ATGCAT 1 cut(s) 104
NspI RCATGY 3 cut(s) 472, 538, 555
PcsI WCGNNNNNNNCGW 2 cut(s) 331, 613
PdmI GAANNNNTTC 1 cut(s) 327
PflFI GACNNNGTC 1 cut(s) 297
Ple19I CGATCG 1 cut(s) 620
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
PsiI TTATAA 1 cut(s) 171
Psp1406I AACGTT 2 cut(s) 325, 334
PspN4I GGNNCC 1 cut(s) 420
PsuI RGATCY 1 cut(s) 418
PsyI GACNNNGTC 1 cut(s) 297
PvuI CGATCG 1 cut(s) 620
RsaI GTAC 5 cut(s) 83, 194, 256, 402, 646
RsaNI GTAC 5 cut(s) 82, 193, 255, 401, 645
RseI CAYNNNNRTG 1 cut(s) 550
SaqAI TTAA 3 cut(s) 134, 203, 480
Sau3AI GATC 2 cut(s) 418, 617
ScaI AGTACT 2 cut(s) 194, 402
SchI GAGTC 1 cut(s) 452
SetI ASST 8 cut(s) 113, 124, 231, 274, 328, 337, 486, 659
SmiMI CAYNNNNRTG 1 cut(s) 550
SmlI CTYRAG 1 cut(s) 460
SmoI CTYRAG 1 cut(s) 460
Sse9I AATT 6 cut(s) 36, 176, 425, 447, 539, 622
SsiI CCGC 2 cut(s) 258, 347
SspI AATATT 1 cut(s) 511
SspMI CTAG 1 cut(s) 658
TaiI ACGT 2 cut(s) 328, 337
TaqI TCGA 3 cut(s) 75, 616, 620
TasI AATT 6 cut(s) 36, 176, 425, 447, 539, 622
TatI WGTACW 3 cut(s) 81, 192, 400
Tru1I TTAA 3 cut(s) 134, 203, 480
Tru9I TTAA 3 cut(s) 134, 203, 480
TscAI CASTG 1 cut(s) 577
TspDTI ATGAA 3 cut(s) 104, 171, 459
TspRI CASTG 1 cut(s) 577
Tth111I GACNNNGTC 1 cut(s) 297
XapI RAATTY 3 cut(s) 425, 447, 622
XceI RCATGY 3 cut(s) 472, 538, 555
XmnI GAANNNNTTC 1 cut(s) 327
XspI CTAG 1 cut(s) 658
ZrmI AGTACT 2 cut(s) 194, 402
Zsp2I ATGCAT 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.