Rw5G021860
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000469
Physical Location & Seq
Forward (+)
29786451 .. 29790454
4004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G021860.1

Sequence Viewer

Length: 1116 bp
ATGGAAGATGGTGACTCAGATAGTTCTTTAGAACTAGATGAAATGGCACAACATATGATAATATGTATGAACATCTATGATTATTGGTCTTCATACATAGACAAGGTTCCTTGCCATACATCGATATTATCTGGAGCTGAATATGTGCAAGAGTTGCTGAATGGACATCCAGATAGAATTTATAACTCATTTCGCATGGATAAACATGTATTTCAAAGGTTGTGTTGCACGCTTGAGAGTTTAAATTTATTAAGTTTAAATTTATTAAAAAATGATCGGCATGTAGGCATTCAAGAGGCCGTGGCTATTTTCTTATATATAGTATCTCATAGTGAGCGCATGAGAATGGCTGCTGAGCGCTTTCAACGGTCAAAAGATACTATTCATCGACAATTTAAGCGTGTATTAGCAGCCTTGTGTAAGTTATCGCCACAGATCATACGTCCTCAAAGTCAAGGAGAAACACCTCCAGAAATTTTGAACAATCCAAAGTTTTATCCATATTTCGAGAAATGTATTGGGGCAATTGATGGAACACATGTCGCTGCATGGGCCCCGGCTCATAAACAAACTTCATATCGTGGTAGGAAGGTCCTTGTTACCCAAAATATCATGTGTGCATGCTCCTTCGACATGATTGTTCGGGACGTAACAATGATGTTCACCTTTGTTTACACTGGATGGGAAGGGACTGCGAATGATTCCAGGGTCTTTGCAGATGCAGTGACCAGGCCAGAGAATAAATTTCCATTTCCAAATGAAGGATATTATTATGTGGTGGATGCTGGATATGCAAACATGCCAGGATTCTTAGCTCCTTATCGAGGTGAGAGGTATCATTTGCGTGATTACAGAGGACCTCGTCGTACACCAAGAGGGCCTAGAGAGTTGTTCAATTACAGACATTCTTCATTGCGAAATGTGATTGAGAGATGTTTTGGTGAAGCTCGTCGCGACAAATTGTTTGAAGCTTTTGACGTAGAAGATATGATTTTTGAGGAGGAAAACAGTACACCAGCGAACTTAGATATGTCACAAGAGAACCTTGCACAAATGGCTAATGTTAGAAATGAAATTGCAGAAGACTTGTGGCAAGATTTTATCCCTCACCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDE_Tnp_4

Protein Analysis

371

Amino Acids

43.12

Weight (kDa)

5.87

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8040 PF26138 40 - 141 5.9e-37 Domain of unknown function (DUF8040)
DDE_Tnp_4 PF13359 176 - 317 5.3e-11 DDE superfamily endonuclease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43722 AT3G30525 AT5G28730
fragaria_vesca FvH4_1g10461 FvH4_1g19401 FvH4_2g04530 FvH4_2g04530 FvH4_2g11121 FvH4_2g13361 FvH4_3g21471 FvH4_3g26161 FvH4_3g29911 FvH4_4g20922 FvH4_4g30624 FvH4_5g23912 FvH4_6g30381 FvH4_6g53026 FvH4_7g00061 FvH4_7g19211 FvH4_7g19211
malus_domestica MD06G1090500.v1.1 MD08G1193900.v1.1 MD11G1151600.v1.1 MD16G1281600.v1.1
prunus_persica Prupe.1G096200_v2.0.a1 Prupe.1G189200_v2.0.a1 Prupe.1G198400_v2.0.a1 Prupe.6G167200_v2.0.a1 Prupe.6G192900_v2.0.a1
pyrus_communis pycom02g00900 pycom06g03500 pycom07g13740 pycom11g10040 pycom17g21680 pycom520g00090
rosa_chinensis RchiOBHm_Chr1g0354781 RchiOBHm_Chr2g0085491 RchiOBHm_Chr2g0103971 RchiOBHm_Chr2g0145791 RchiOBHm_Chr4g0414051 RchiOBHm_Chr4g0419691 RchiOBHm_Chr4g0435871 RchiOBHm_Chr6g0292381 RchiOBHm_Chr7g0199291
rosa_laevigata RLG00000035604
rosa_multiflora Rmu_co8287949.1_g000001 Rmu_sc0000079.1_g000057 Rmu_sc0000240.1_g000063 Rmu_sc0000552.1_g000020 Rmu_sc0000950.1_g000002 Rmu_sc0001257.1_g000003 Rmu_sc0001597.1_g000014 Rmu_sc0001939.1_g000006 Rmu_sc0002160.1_g000015 Rmu_sc0003526.1_g000015 Rmu_sc0005712.1_g000025 Rmu_sc0006032.1_g000011 Rmu_sc0006935.1_g000005 Rmu_sc0008636.1_g000002 Rmu_sc0009205.1_g000006 Rmu_sc0009438.1_g000007 Rmu_ssc0000486.1_g000013
rosa_roxburghii Rroxscaffold_1G00003870 Rroxscaffold_1G00014970 Rroxscaffold_3G00218750 Rroxscaffold_5G00367160 Rroxscaffold_7G00186480
rosa_rugosa Rorug05G0192000
rosa_samantha Rh4BG219200
rosa_wichuraiana Rw1G010350 Rw1G014480 Rw1G023690 Rw1G033480 Rw2G050290 Rw3G027480 Rw4G033650 Rw5G005670 Rw5G021010 Rw5G021860 Rw5G041260 Rw5G041760 Rw6G008410 Rw6G021080 Rw6G022950 Rw6G025480 Rw6G028700 Rw7G002710 Rw7G004940 Rw7G031510 Rw7G041110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 183
AccII CGCG 1 cut(s) 954
AcsI RAATTY 5 cut(s) 177, 244, 259, 474, 743
AfaI GTAC 2 cut(s) 868, 1012
AfeI AGCGCT 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 761, 824
AflIII ACRYGT 2 cut(s) 205, 538
AgsI TTSAA 6 cut(s) 215, 293, 365, 481, 895, 968
AjnI CCWGG 3 cut(s) 704, 728, 802
AluBI AGCT 4 cut(s) 137, 815, 947, 971
AluI AGCT 4 cut(s) 137, 815, 947, 971
Aor51HI AGCGCT 1 cut(s) 359
AoxI GGCC 4 cut(s) 297, 552, 731, 878
ApaI GGGCCC 1 cut(s) 556
ApeKI GCWGC 3 cut(s) 350, 410, 545
ApoI RAATTY 5 cut(s) 177, 244, 259, 474, 743
ArsI GACNNNNNNTTYG 2 cut(s) 947, 979
AspLEI GCGC 2 cut(s) 339, 360
AspS9I GGNCC 5 cut(s) 552, 553, 592, 857, 878
AsuC2I CCSGG 1 cut(s) 557
AsuHPI GGTGA 5 cut(s) 23, 655, 839, 953, 1100
AvaII GGWCC 2 cut(s) 592, 857
BaeGI GKGCMC 1 cut(s) 556
BanII GRGCYC 1 cut(s) 556
BbsI GAAGAC 2 cut(s) 81, 1089
BbvI GCAGC 3 cut(s) 337, 422, 532
BccI CCATC 3 cut(s) 2, 524, 675
BceAI ACGGC 1 cut(s) 284
BciT130I CCWGG 3 cut(s) 706, 730, 804
BcnI CCSGG 1 cut(s) 557
BfaI CTAG 2 cut(s) 35, 882
BfoI RGCGCY 1 cut(s) 361
BisI GCNGC 3 cut(s) 351, 411, 546
BlpI GCTNAGC 1 cut(s) 354
BlsI GCNGC 3 cut(s) 352, 412, 547
Bme1390I CCNGG 4 cut(s) 557, 706, 730, 804
Bme18I GGWCC 2 cut(s) 592, 857
BmgT120I GGNCC 5 cut(s) 552, 553, 592, 857, 878
BmiI GGNNCC 3 cut(s) 108, 554, 555
BmrFI CCNGG 4 cut(s) 557, 706, 730, 804
BmsI GCATC 2 cut(s) 709, 772
BpiI GAAGAC 2 cut(s) 81, 1089
BpmI CTGGAG 2 cut(s) 153, 453
Bpu1102I GCTNAGC 1 cut(s) 354
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 557
Bsa29I ATCGAT 1 cut(s) 122
BsaJI CCNNGG 3 cut(s) 300, 555, 705
Bsc4I CCNNNNNNNGG 2 cut(s) 761, 824
Bse1I ACTGG 1 cut(s) 682
Bse3DI GCAATG 1 cut(s) 911
BseBI CCWGG 3 cut(s) 706, 730, 804
BseCI ATCGAT 1 cut(s) 122
BseDI CCNNGG 3 cut(s) 300, 555, 705
BseGI GGATG 3 cut(s) 166, 686, 787
BseLI CCNNNNNNNGG 2 cut(s) 761, 824
BseMI GCAATG 1 cut(s) 911
BseMII CTCAG 2 cut(s) 30, 345
BseNI ACTGG 1 cut(s) 682
BseRI GAGGAG 1 cut(s) 1013
BseSI GKGCMC 1 cut(s) 556
BseXI GCAGC 3 cut(s) 337, 422, 532
Bsh1236I CGCG 1 cut(s) 954
BshFI GGCC 4 cut(s) 299, 554, 733, 880
BshVI ATCGAT 1 cut(s) 122
BsiSI CCGG 1 cut(s) 557
BslFI GGGAC 2 cut(s) 659, 703
BslI CCNNNNNNNGG 2 cut(s) 761, 824
BsmFI GGGAC 2 cut(s) 659, 703
BsmI GAATGC 1 cut(s) 288
BsnI GGCC 4 cut(s) 299, 554, 733, 880
Bsp120I GGGCCC 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 556
Bsp143I GATC 2 cut(s) 274, 435
Bsp1720I GCTNAGC 1 cut(s) 354
Bsp68I TCGCGA 1 cut(s) 954
BspANI GGCC 4 cut(s) 299, 554, 733, 880
BspCNI CTCAG 2 cut(s) 29, 346
BspDI ATCGAT 1 cut(s) 122
BspFNI CGCG 1 cut(s) 954
BspLI GGNNCC 3 cut(s) 108, 554, 555
BsrDI GCAATG 1 cut(s) 911
BsrI ACTGG 1 cut(s) 682
BssECI CCNNGG 3 cut(s) 300, 555, 705
BssMI GATC 2 cut(s) 274, 435
Bst2UI CCWGG 3 cut(s) 706, 730, 804
Bst4CI ACNGT 2 cut(s) 369, 1010
BstAPI GCANNNNNTGC 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 230, 622
BstDEI CTNAG 5 cut(s) 16, 354, 811, 1024, 1113
BstDSI CCRYGG 1 cut(s) 300
BstENI CCTNNNNNAGG 1 cut(s) 822
BstF5I GGATG 3 cut(s) 166, 686, 787
BstFNI CGCG 1 cut(s) 954
BstH2I RGCGCY 1 cut(s) 361
BstHHI GCGC 2 cut(s) 339, 360
BstKTI GATC 2 cut(s) 277, 438
BstMBI GATC 2 cut(s) 274, 435
BstMWI GCNNNNNNNGC 4 cut(s) 154, 551, 791, 1055
BstNI CCWGG 3 cut(s) 706, 730, 804
BstNSI RCATGY 5 cut(s) 209, 284, 542, 624, 802
BstSCI CCNGG 4 cut(s) 555, 704, 728, 802
BstSLI GKGCMC 1 cut(s) 556
BstUI CGCG 1 cut(s) 954
BstV1I GCAGC 3 cut(s) 337, 422, 532
BstV2I GAAGAC 2 cut(s) 81, 1089
Bsu15I ATCGAT 1 cut(s) 122
BsuRI GGCC 4 cut(s) 299, 554, 733, 880
BsuTUI ATCGAT 1 cut(s) 122
BtgI CCRYGG 1 cut(s) 300
BtsCI GGATG 3 cut(s) 166, 686, 787
BtsI GCAGTG 1 cut(s) 729
BtsIMutI CAGTG 2 cut(s) 675, 729
BtuMI TCGCGA 1 cut(s) 954
Cac8I GCNNGC 2 cut(s) 230, 622
CfoI GCGC 2 cut(s) 339, 360
Cfr13I GGNCC 5 cut(s) 552, 553, 592, 857, 878
ClaI ATCGAT 1 cut(s) 122
Csp6I GTAC 2 cut(s) 867, 1011
CviQI GTAC 2 cut(s) 867, 1011
DdeI CTNAG 5 cut(s) 16, 354, 811, 1024, 1113
DpnI GATC 2 cut(s) 276, 437
DpnII GATC 2 cut(s) 274, 435
DraI TTTAAA 2 cut(s) 243, 258
Eco24I GRGCYC 1 cut(s) 556
Eco47I GGWCC 2 cut(s) 592, 857
Eco47III AGCGCT 1 cut(s) 359
EcoNI CCTNNNNNAGG 1 cut(s) 822
EcoO109I RGGNCCY 4 cut(s) 553, 592, 857, 878
EcoRII CCWGG 3 cut(s) 704, 728, 802
EcoT38I GRGCYC 1 cut(s) 556
FalI AAGNNNNNCTT 2 cut(s) 1029, 1061
FaqI GGGAC 2 cut(s) 659, 703
FauNDI CATATG 1 cut(s) 54
Fnu4HI GCNGC 3 cut(s) 351, 411, 546
FokI GGATG 3 cut(s) 153, 693, 794
FriOI GRGCYC 1 cut(s) 556
Fsp4HI GCNGC 3 cut(s) 351, 411, 546
FspBI CTAG 2 cut(s) 35, 882
GlaI GCGC 2 cut(s) 338, 359
GluI GCNGC 3 cut(s) 351, 411, 546
GsuI CTGGAG 2 cut(s) 153, 453
HaeII RGCGCY 1 cut(s) 361
HaeIII GGCC 4 cut(s) 299, 554, 733, 880
HapII CCGG 1 cut(s) 557
HhaI GCGC 2 cut(s) 339, 360
Hin6I GCGC 2 cut(s) 337, 358
HinP1I GCGC 2 cut(s) 337, 358
HindIII AAGCTT 1 cut(s) 969
HinfI GANTC 3 cut(s) 14, 701, 807
HpaII CCGG 1 cut(s) 557
HphI GGTGA 5 cut(s) 23, 655, 839, 953, 1100
Hpy166II GTNNAC 4 cut(s) 663, 673, 869, 1013
Hpy188I TCNGA 1 cut(s) 19
Hpy188III TCNNGA 7 cut(s) 132, 170, 293, 470, 508, 644, 953
Hpy8I GTNNAC 4 cut(s) 663, 673, 869, 1013
Hpy99I CGWCG 2 cut(s) 867, 954
HpyAV CCTTC 4 cut(s) 583, 637, 680, 755
HpyCH4III ACNGT 2 cut(s) 369, 1010
HpyCH4IV ACGT 3 cut(s) 442, 648, 978
HpyCH4V TGCA 9 cut(s) 148, 228, 548, 620, 716, 722, 794, 1049, 1079
HpyF10VI GCNNNNNNNGC 4 cut(s) 154, 551, 791, 1055
HpyF3I CTNAG 5 cut(s) 16, 354, 811, 1024, 1113
HpySE526I ACGT 3 cut(s) 442, 648, 978
HspAI GCGC 2 cut(s) 337, 358
Kzo9I GATC 2 cut(s) 274, 435
LmnI GCTCC 3 cut(s) 134, 629, 820
Lsp1109I GCAGC 3 cut(s) 337, 422, 532
LweI GCATC 2 cut(s) 709, 772
MaeI CTAG 2 cut(s) 35, 882
MaeII ACGT 3 cut(s) 442, 648, 978
MaeIII GTNAC 5 cut(s) 11, 598, 649, 724, 1032
MalI GATC 2 cut(s) 276, 437
MboI GATC 2 cut(s) 274, 435
MboII GAAGA 5 cut(s) 17, 81, 900, 995, 1094
MfeI CAATTG 1 cut(s) 525
MhlI GDGCHC 1 cut(s) 556
MlyI GAGTC 1 cut(s) 8
MseI TTAA 5 cut(s) 242, 251, 257, 266, 396
MslI CAYNNNNRTG 2 cut(s) 344, 843
MspI CCGG 1 cut(s) 557
MspR9I CCNGG 4 cut(s) 557, 706, 730, 804
MunI CAATTG 1 cut(s) 525
Mva1269I GAATGC 1 cut(s) 288
MvaI CCWGG 3 cut(s) 706, 730, 804
MvnI CGCG 1 cut(s) 954
MwoI GCNNNNNNNGC 4 cut(s) 154, 551, 791, 1055
NciI CCSGG 1 cut(s) 557
NdeI CATATG 1 cut(s) 54
NdeII GATC 2 cut(s) 274, 435
NlaIV GGNNCC 3 cut(s) 108, 554, 555
NmuCI GTSAC 3 cut(s) 11, 724, 1032
NruI TCGCGA 1 cut(s) 954
NspI RCATGY 5 cut(s) 209, 284, 542, 624, 802
PaeI GCATGC 1 cut(s) 624
PciI ACATGT 2 cut(s) 205, 538
PctI GAATGC 1 cut(s) 288
PfeI GAWTC 2 cut(s) 701, 807
PflFI GACNNNGTC 1 cut(s) 861
PkrI GCNGC 3 cut(s) 352, 412, 547
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
PpuMI RGGWCCY 2 cut(s) 592, 857
PscI ACATGT 2 cut(s) 205, 538
PsiI TTATAA 1 cut(s) 183
Psp5II RGGWCCY 2 cut(s) 592, 857
Psp6I CCWGG 3 cut(s) 704, 728, 802
PspGI CCWGG 3 cut(s) 704, 728, 802
PspN4I GGNNCC 3 cut(s) 108, 554, 555
PspOMI GGGCCC 1 cut(s) 552
PspPI GGNCC 5 cut(s) 552, 553, 592, 857, 878
PspPPI RGGWCCY 2 cut(s) 592, 857
PsyI GACNNNGTC 1 cut(s) 861
RruI TCGCGA 1 cut(s) 954
RsaI GTAC 2 cut(s) 868, 1012
RsaNI GTAC 2 cut(s) 867, 1011
RseI CAYNNNNRTG 2 cut(s) 344, 843
SaqAI TTAA 5 cut(s) 242, 251, 257, 266, 396
SatI GCNGC 3 cut(s) 351, 411, 546
Sau3AI GATC 2 cut(s) 274, 435
Sau96I GGNCC 5 cut(s) 552, 553, 592, 857, 878
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 4 cut(s) 557, 706, 730, 804
SduI GDGCHC 1 cut(s) 556
SfaNI GCATC 2 cut(s) 709, 772
SinI GGWCC 2 cut(s) 592, 857
SmiMI CAYNNNNRTG 2 cut(s) 344, 843
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
SphI GCATGC 1 cut(s) 624
SspMI CTAG 2 cut(s) 35, 882
StyD4I CCNGG 4 cut(s) 555, 704, 728, 802
TaaI ACNGT 2 cut(s) 369, 1010
TaiI ACGT 3 cut(s) 445, 651, 981
TaqI TCGA 5 cut(s) 122, 388, 507, 630, 823
TatI WGTACW 1 cut(s) 1010
TfiI GAWTC 2 cut(s) 701, 807
Tru1I TTAA 5 cut(s) 242, 251, 257, 266, 396
Tru9I TTAA 5 cut(s) 242, 251, 257, 266, 396
TscAI CASTG 2 cut(s) 682, 729
TseFI GTSAC 3 cut(s) 11, 724, 1032
TseI GCWGC 3 cut(s) 350, 410, 545
Tsp45I GTSAC 3 cut(s) 11, 724, 1032
TspDTI ATGAA 8 cut(s) 54, 81, 83, 374, 564, 774, 900, 1086
TspRI CASTG 2 cut(s) 682, 729
Tth111I GACNNNGTC 1 cut(s) 861
VpaK11BI GGWCC 2 cut(s) 592, 857
XagI CCTNNNNNAGG 1 cut(s) 822
XapI RAATTY 5 cut(s) 177, 244, 259, 474, 743
XceI RCATGY 5 cut(s) 209, 284, 542, 624, 802
XspI CTAG 2 cut(s) 35, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.