Prupe.1G198400_v2.0.a1
ERF Family

DDE superfamily endonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000469
Physical Location & Seq
Forward (+)
19068206 .. 19069079
874 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G198400.1

Sequence Viewer

Length: 765 bp
ATGCAAGTTGTAGAAGGCATACATGAAATTGTTGAGGATGATAAGGTTTTCGTGAAAGCTGCATATGTCTTGACTGAAAGAAAGAATAGAGAGATGTTTGTTGCGTTGAGGCAAGCCAACCGACAAATTATGTGGCTTAGGAAGAAATTACGTGACAAGCTAACCGATAAATGGGTCATGGATGTTTTAAATGGTCACAAGATAAGGTGCTATGAACAATTTAGGATGGAAAAACATGTCTTCATAAAATTGTTAGAGACACTTACAAGTGCATATGATTTGAAAGAAGTTGGAGATATACCAGTAGTTGAGGTATTATTCCTCATCATTTTAGGTCATGGGTTCACTTATAGAATGGTTCAAGAAAGGTTCCAACATTCTGGTGAGACAGTGTCAAAGTGGTTTGGGATAATTTTAGATGTTTTTTGTCCCATGGCTAGTTATATCATAAGTCCACAAGACCCACATTTTAGGAGAGTTCCGGGTAAAATAAAAGTTGATGATCGATATTGGCCATACTTCAAAGATTGCATAGGGGCAATTGATGGTACGCATATACCACTGGTCATGCCTAGAGCAAGACAAGTACTCTATATTGAAAGAAAAGGTATCACTACCCAAAGTGTTATGGTTGTATGGAATTTTAATATATGTTTTACATTTGTATGGGCTGGATGGGAAAGTGCTGTTCACAATGCACGAATATTCATGGAGGCCTTACGAAGGCCAATTTTAAAGTTTCGTCATCCACCTACTGGTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

255

Amino Acids

30.0

Weight (kDa)

9.41

Isoelectric Point (pI)

31.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000469)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G43722 AT3G30525 AT5G28730
fragaria_vesca FvH4_1g10461 FvH4_1g19401 FvH4_2g04530 FvH4_2g04530 FvH4_2g11121 FvH4_2g13361 FvH4_3g21471 FvH4_3g26161 FvH4_3g29911 FvH4_4g20922 FvH4_4g30624 FvH4_5g23912 FvH4_6g30381 FvH4_6g53026 FvH4_7g00061 FvH4_7g19211 FvH4_7g19211
malus_domestica MD06G1090500.v1.1 MD08G1193900.v1.1 MD11G1151600.v1.1 MD16G1281600.v1.1
prunus_persica Prupe.1G096200_v2.0.a1 Prupe.1G189200_v2.0.a1 Prupe.1G198400_v2.0.a1 Prupe.6G167200_v2.0.a1 Prupe.6G192900_v2.0.a1
pyrus_communis pycom02g00900 pycom06g03500 pycom07g13740 pycom11g10040 pycom17g21680 pycom520g00090
rosa_chinensis RchiOBHm_Chr1g0354781 RchiOBHm_Chr2g0085491 RchiOBHm_Chr2g0103971 RchiOBHm_Chr2g0145791 RchiOBHm_Chr4g0414051 RchiOBHm_Chr4g0419691 RchiOBHm_Chr4g0435871 RchiOBHm_Chr6g0292381 RchiOBHm_Chr7g0199291
rosa_laevigata RLG00000035604
rosa_multiflora Rmu_co8287949.1_g000001 Rmu_sc0000079.1_g000057 Rmu_sc0000240.1_g000063 Rmu_sc0000552.1_g000020 Rmu_sc0000950.1_g000002 Rmu_sc0001257.1_g000003 Rmu_sc0001597.1_g000014 Rmu_sc0001939.1_g000006 Rmu_sc0002160.1_g000015 Rmu_sc0003526.1_g000015 Rmu_sc0005712.1_g000025 Rmu_sc0006032.1_g000011 Rmu_sc0006935.1_g000005 Rmu_sc0008636.1_g000002 Rmu_sc0009205.1_g000006 Rmu_sc0009438.1_g000007 Rmu_ssc0000486.1_g000013
rosa_roxburghii Rroxscaffold_1G00003870 Rroxscaffold_1G00014970 Rroxscaffold_3G00218750 Rroxscaffold_5G00367160 Rroxscaffold_7G00186480
rosa_rugosa Rorug05G0192000
rosa_samantha Rh4BG219200
rosa_wichuraiana Rw1G010350 Rw1G014480 Rw1G023690 Rw1G033480 Rw2G050290 Rw3G027480 Rw4G033650 Rw5G005670 Rw5G021010 Rw5G021860 Rw5G041260 Rw5G041760 Rw6G008410 Rw6G021080 Rw6G022950 Rw6G025480 Rw6G028700 Rw7G002710 Rw7G004940 Rw7G031510 Rw7G041110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 755
AcoI YGGCCR 1 cut(s) 512
AcsI RAATTY 1 cut(s) 640
AfaI GTAC 2 cut(s) 550, 588
AfiI CCNNNNNNNGG 3 cut(s) 171, 723, 755
AflIII ACRYGT 1 cut(s) 235
AgsI TTSAA 4 cut(s) 283, 362, 523, 599
AluBI AGCT 2 cut(s) 59, 160
AluI AGCT 2 cut(s) 59, 160
Alw26I GTCTC 2 cut(s) 251, 380
AoxI GGCC 3 cut(s) 512, 714, 725
ApeKI GCWGC 1 cut(s) 59
ApoI RAATTY 1 cut(s) 640
AsuC2I CCSGG 1 cut(s) 483
AsuHPI GGTGA 1 cut(s) 395
BalI TGGCCA 1 cut(s) 514
BbsI GAAGAC 1 cut(s) 232
BbvI GCAGC 1 cut(s) 46
BccI CCATC 3 cut(s) 220, 539, 669
BcnI CCSGG 1 cut(s) 483
BcoDI GTCTC 2 cut(s) 251, 380
BfaI CTAG 2 cut(s) 438, 573
BisI GCNGC 1 cut(s) 60
BlsI GCNGC 1 cut(s) 61
BmcAI AGTACT 1 cut(s) 588
Bme1390I CCNGG 1 cut(s) 483
BmiI GGNNCC 1 cut(s) 371
BmrFI CCNGG 1 cut(s) 483
BpiI GAAGAC 1 cut(s) 232
Bpu10I CCTNAGC 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 483
Bsa29I ATCGAT 1 cut(s) 505
BsaAI YACGTR 1 cut(s) 152
BsaJI CCNNGG 1 cut(s) 432
Bsc4I CCNNNNNNNGG 3 cut(s) 171, 723, 755
Bse1I ACTGG 3 cut(s) 302, 567, 760
BseCI ATCGAT 1 cut(s) 505
BseDI CCNNGG 1 cut(s) 432
BseGI GGATG 5 cut(s) 43, 187, 231, 680, 745
BseLI CCNNNNNNNGG 3 cut(s) 171, 723, 755
BseNI ACTGG 3 cut(s) 302, 567, 760
BseXI GCAGC 1 cut(s) 46
BshFI GGCC 3 cut(s) 514, 716, 727
BshVI ATCGAT 1 cut(s) 505
BsiSI CCGG 1 cut(s) 482
BslFI GGGAC 1 cut(s) 414
BslI CCNNNNNNNGG 3 cut(s) 171, 723, 755
BsmAI GTCTC 2 cut(s) 251, 380
BsmFI GGGAC 1 cut(s) 414
BsnI GGCC 3 cut(s) 514, 716, 727
Bsp143I GATC 1 cut(s) 502
Bsp19I CCATGG 1 cut(s) 432
BspANI GGCC 3 cut(s) 514, 716, 727
BspDI ATCGAT 1 cut(s) 505
BspLI GGNNCC 1 cut(s) 371
BsrI ACTGG 3 cut(s) 302, 567, 760
BssECI CCNNGG 1 cut(s) 432
BssMI GATC 1 cut(s) 502
BssT1I CCWWGG 1 cut(s) 432
Bst4CI ACNGT 1 cut(s) 391
BstBAI YACGTR 1 cut(s) 152
BstC8I GCNNGC 1 cut(s) 114
BstDEI CTNAG 1 cut(s) 137
BstDSI CCRYGG 1 cut(s) 432
BstENI CCTNNNNNAGG 1 cut(s) 721
BstF5I GGATG 5 cut(s) 43, 187, 231, 680, 745
BstKTI GATC 1 cut(s) 505
BstMAI GTCTC 2 cut(s) 251, 380
BstMBI GATC 1 cut(s) 502
BstNSI RCATGY 1 cut(s) 239
BstSCI CCNGG 1 cut(s) 481
BstV1I GCAGC 1 cut(s) 46
BstV2I GAAGAC 1 cut(s) 232
BstXI CCANNNNNNTGG 1 cut(s) 380
Bsu15I ATCGAT 1 cut(s) 505
BsuRI GGCC 3 cut(s) 514, 716, 727
BsuTUI ATCGAT 1 cut(s) 505
BtgI CCRYGG 1 cut(s) 432
BtsCI GGATG 5 cut(s) 43, 187, 231, 680, 745
BtsIMutI CAGTG 2 cut(s) 396, 560
Cac8I GCNNGC 1 cut(s) 114
ClaI ATCGAT 1 cut(s) 505
Csp6I GTAC 2 cut(s) 549, 587
CspCI CAANNNNNGTGG 2 cut(s) 113, 148
CviAII CATG 7 cut(s) 23, 178, 236, 338, 433, 568, 709
CviJI RGCY 9 cut(s) 59, 116, 136, 160, 437, 514, 671, 716, 727
CviKI_1 RGCY 9 cut(s) 59, 116, 136, 160, 437, 514, 671, 716, 727
CviQI GTAC 2 cut(s) 549, 587
DdeI CTNAG 1 cut(s) 137
DpnI GATC 1 cut(s) 504
DpnII GATC 1 cut(s) 502
DraI TTTAAA 2 cut(s) 189, 735
EaeI YGGCCR 1 cut(s) 512
Eco130I CCWWGG 1 cut(s) 432
Eco147I AGGCCT 1 cut(s) 716
EcoNI CCTNNNNNAGG 1 cut(s) 721
EcoT14I CCWWGG 1 cut(s) 432
ErhI CCWWGG 1 cut(s) 432
FaeI CATG 7 cut(s) 26, 181, 239, 341, 436, 571, 712
FaqI GGGAC 1 cut(s) 414
FatI CATG 7 cut(s) 22, 177, 235, 337, 432, 567, 708
FauNDI CATATG 2 cut(s) 64, 274
Fnu4HI GCNGC 1 cut(s) 60
FokI GGATG 5 cut(s) 50, 194, 238, 687, 732
Fsp4HI GCNGC 1 cut(s) 60
FspBI CTAG 2 cut(s) 438, 573
GluI GCNGC 1 cut(s) 60
HaeIII GGCC 3 cut(s) 514, 716, 727
HapII CCGG 1 cut(s) 482
Hin1II CATG 7 cut(s) 26, 181, 239, 341, 436, 571, 712
HpaII CCGG 1 cut(s) 482
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 3 cut(s) 345, 455, 691
Hpy188III TCNNGA 3 cut(s) 52, 70, 362
Hpy8I GTNNAC 3 cut(s) 345, 455, 691
HpyAV CCTTC 2 cut(s) 8, 717
HpyCH4III ACNGT 1 cut(s) 391
HpyCH4IV ACGT 1 cut(s) 151
HpyCH4V TGCA 5 cut(s) 4, 62, 272, 531, 698
HpyF3I CTNAG 1 cut(s) 137
HpySE526I ACGT 1 cut(s) 151
Hsp92II CATG 7 cut(s) 26, 181, 239, 341, 436, 571, 712
Kzo9I GATC 1 cut(s) 502
LpnPI CCDG 6 cut(s) 315, 366, 495, 548, 657, 741
Lsp1109I GCAGC 1 cut(s) 46
MaeI CTAG 2 cut(s) 438, 573
MaeII ACGT 1 cut(s) 151
MaeIII GTNAC 3 cut(s) 152, 194, 758
MalI GATC 1 cut(s) 504
MboI GATC 1 cut(s) 502
MboII GAAGA 2 cut(s) 154, 232
MfeI CAATTG 1 cut(s) 540
MlsI TGGCCA 1 cut(s) 514
MluCI AATT 9 cut(s) 27, 126, 146, 218, 248, 411, 540, 640, 729
MluNI TGGCCA 1 cut(s) 514
MmeI TCCRAC 2 cut(s) 271, 397
MnlI CCTC 5 cut(s) 28, 102, 304, 332, 706
Mox20I TGGCCA 1 cut(s) 514
MscI TGGCCA 1 cut(s) 514
MseI TTAA 3 cut(s) 188, 645, 734
MslI CAYNNNNRTG 2 cut(s) 381, 664
Msp20I TGGCCA 1 cut(s) 514
MspI CCGG 1 cut(s) 482
MspR9I CCNGG 1 cut(s) 483
MunI CAATTG 1 cut(s) 540
NciI CCSGG 1 cut(s) 483
NcoI CCATGG 1 cut(s) 432
NdeI CATATG 2 cut(s) 64, 274
NdeII GATC 1 cut(s) 502
NlaIII CATG 7 cut(s) 26, 181, 239, 341, 436, 571, 712
NlaIV GGNNCC 1 cut(s) 371
NmuCI GTSAC 2 cut(s) 152, 194
NspI RCATGY 1 cut(s) 239
PceI AGGCCT 1 cut(s) 716
PciI ACATGT 1 cut(s) 235
PflFI GACNNNGTC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 755
PkrI GCNGC 1 cut(s) 61
Ppu21I YACGTR 1 cut(s) 152
PscI ACATGT 1 cut(s) 235
PspN4I GGNNCC 1 cut(s) 371
PsyI GACNNNGTC 1 cut(s) 391
RsaI GTAC 2 cut(s) 550, 588
RsaNI GTAC 2 cut(s) 549, 587
RseI CAYNNNNRTG 2 cut(s) 381, 664
SaqAI TTAA 3 cut(s) 188, 645, 734
SatI GCNGC 1 cut(s) 60
Sau3AI GATC 1 cut(s) 502
ScaI AGTACT 1 cut(s) 588
ScrFI CCNGG 1 cut(s) 483
SmiMI CAYNNNNRTG 2 cut(s) 381, 664
Sse9I AATT 9 cut(s) 27, 126, 146, 218, 248, 411, 540, 640, 729
SseBI AGGCCT 1 cut(s) 716
SspI AATATT 1 cut(s) 705
SspMI CTAG 2 cut(s) 438, 573
StuI AGGCCT 1 cut(s) 716
StyD4I CCNGG 1 cut(s) 481
StyI CCWWGG 1 cut(s) 432
TaaI ACNGT 1 cut(s) 391
TaiI ACGT 1 cut(s) 154
TaqI TCGA 1 cut(s) 505
TasI AATT 9 cut(s) 27, 126, 146, 218, 248, 411, 540, 640, 729
TatI WGTACW 1 cut(s) 586
Tru1I TTAA 3 cut(s) 188, 645, 734
Tru9I TTAA 3 cut(s) 188, 645, 734
TscAI CASTG 2 cut(s) 396, 567
TseFI GTSAC 2 cut(s) 152, 194
TseI GCWGC 1 cut(s) 59
Tsp45I GTSAC 2 cut(s) 152, 194
TspDTI ATGAA 4 cut(s) 39, 228, 232, 697
TspRI CASTG 2 cut(s) 396, 567
Tth111I GACNNNGTC 1 cut(s) 391
Van91I CCANNNNNTGG 1 cut(s) 755
XagI CCTNNNNNAGG 1 cut(s) 721
XapI RAATTY 1 cut(s) 640
XceI RCATGY 1 cut(s) 239
XspI CTAG 2 cut(s) 438, 573
ZrmI AGTACT 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.