Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb3

Orthogroup ID

OG0000350

Physical Location & Seq

Genomic Coordinates Forward (+)

28335679 .. 28339154

Gene Structure 3476 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_3g32960.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2766 bp

ATGTCTTCTCCCTCTGCAAACTTTTACAAAGTCAAAACGATTTATTGGCTCTTGTCTTCTCTGCTTCTCTATTTGTTCATCATTCTCTCCAATGGAGTTGCAGCTCAAAATGGTATCAGTGTCACAAACGTTGGAGCAATCATTGATCTTAATTCTCGTATCGGTAGAGAGCAGAAAGCTGCCATGGAAATAGCAGCTGAAAGCTTCAACAACAAGTCCAACACTCATGAGCTACTCATTCATTTTCGAGACTCAGAAGGAAACCCCTTTCTTGCTGGTTCTGCTGCTGAAGAACTGTTGAAGGAAGAAAAGGTACAAGTGATTGTTGGCATGGAGTATTGGCCAGAAGCAGTCCTAGTAGCTGATCATGTTAGAAACCAATCTCAAGTTGTGCCGGTAATTTCATTTGGTGCACCTCTTATAACTCCACCACTGATCCAACGCCGATGGCCTTTCTTGATACGTATGACCAGTGATGGTTCAGCTCAAATGAAATGCATTGCAGACATAGTTGCTGCATACAATTGGAGAAGGGTTGTGGTGATATACGAAGACGACGGTTATGGTGGTTATGTTGGCTTGCTAGGTCTTTTATCTGAGGCTCTTCAAGATGCTAATGCAAAGATTGAGCATCATTTGGTTCTTCCGAGACTATCTTCAGTGTCTGAGCCTAATTTTGATGAACTAGAAGAGTTGATGAAGCTTCCTACCTTTCAGTCTCGGGTGTTTATTGTTCTTCAATCATCTTTACCAACAGTGAATAATCTTTTCAGAGTGGCTAAGAAGATGGGACTTGTAGGGAGAGACTCAGCTTGGATATTTACAGAATGTATCACTAGTTTGCTTGCTCCTCAAGGTAACAACGATATGGAAGGAGCTCTAGGAATCAGGACTTACTACAGTGAGTCTAGTTCTTATGATGAGTTCCAGAAGGAATTCCAAGTTAAGTATGCAGAAGGAAATAACTCCAAGCCAGGAATTTATACTCTTCGAGCATACGATACTATTAATGTCATTTCAAAAGCCATAGAAAGAATGCCTAGTAACACCAGTAGTACTAACCTTCAAGGTATGATAACTACATTGTTGAGCAATTACAAGGGTTTAAGTGGGAATATGCACTTGAAAGAAGGTGAAGCACAGTTGGATTCTCCGAGACTGAGGATCCTTAATGTTGTCGATGGGAAGATGAACACAGAGTTAAACTTCTGGACACCAAATGGGTTTTCAGAAGGCCTTGGCACAAGTATAACAGATGTTGTTGGTAAAGTGAAATGGCCTGGGAACCTAAGACGTACCCCGAAGGGATGGGCAATGCCTAGTGTTGAAAAGCCAATGATAATTGGGGTTCCAGGAGAAACTTCCTTCAGCAAATTTGTAAAAGTGGAGTATAACAAAGACAATCCAGATGAGAGTACCTATGATGGTTTCTGCATTCAAATTTTCTACAAGGTTTTAAGTCGTTTGAATTATCCTCTTCCGTATGAGTTTCAGGCTGACAATGGAACCTATGATGAACTGGTTGAAAAAGTCCATAACAAGACGTATGATGCTGTGGTTGGTGATATAACTGTTCTCGCTGACCGAATGGACAAGGTGGAATTCACTCAGCCCTATATGGGGTCAGGGTTGTCAATGATAGTACCAGAAGTAACTGATGAGGAGGCAACATGGATGTTTATGAAACCTTTCACATGGCAAATGTGGGTGGTCACCGGTTCCATATTAATCTACACAATGTTGATAGTATGGTTCCTAGAGTGCCCATCTAATCCTGACTTTGGTGGTCCATTGAAGAATCAGATTGGTACAGCAGTTTGGTTCACCTTCTCCTCTTTGTTCTTTGCTCACAGAGAAAAAGTCTATAGCAACTTAACTCGAGTAGTTTTTGTTGTATGGCTGTTTGTCGTATTGGTCCTAACCTCAAGCTACACTGCTAATCTCTCTTCAATGCTCACCATTCAACGCCTGCAACCGAATGTAACTGACATCCAAATGCTACTGAACACCAATGCACCGGTTGGTTTGGATGGGGATTCATTCGTGTACAATTACATGGAGAATGTTCTTAAATTCAAGCGAGATAACTTGATCAAGGTATTGAGTGAATACAGCTATACAGACAACTTCCAAAACAAAACAATATCAGCTGCCTTTCTTGAACTCCCCTATGCTGACGTATTCATGAACAAGTATTGTGAGGGATATACTTCTACCACTCCTACCTTCAGATTTGGAGGCTTAACCTTCATATTTCAGAAAGGCTCTCCAATTGCCAGGGATTTCACTAAGGCCATACTAGAGCTATTGGAGAACGGAGAGATTAAGAAACTTCAAAATGAGTGGTTGACTCCAGAGGAGGAGTGCTCAAAGAATTCAACCTCCAATCAACCAAAAAGCTTGAGCCTCAACAGCTTTTCTGGCCTCTATGTAATATCCGGTGCTACTTCTACATTCTGTTTTCTACTATCTCTTGCTATATGGATGAGGAGGTTTCAGCTCCAAGAAACAAATGCAGGCAGTGCAAGTCCAAGTCCAAGTAATGAGAATATTTGGAACAAAACTGTTAGAATAGTGAAGTTTTTTAAACTTCGAGATCTTGAAATTCCAAATAGAGCTCCAACTTTTGCTAGTTATGTTGTGGATTGGACTACTCCAAGGTGGGGGGAATACTCACCTACTTCTAACAATCCGGAGCAACCTCCGGTTTTCACGCCAGCAGAAATTGAATGCATGCCAGTAGAACAGACAGAAACAAGGGTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

922

Amino Acids

103.81

Weight (kDa)

5.27

Isoelectric Point (pI)

42.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	54 - 373	7.8e-15	Periplasmic binding protein
ANF_receptor	PF01094	59 - 393	3.1e-41	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	459 - 549	6.2e-09	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	470 - 784	4e-14	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	565 - 815	1.2e-31	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000350)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g04591 FvH4_1g04600 FvH4_3g08210 FvH4_3g08210 FvH4_3g08211 FvH4_3g32960 FvH4_3g32961 FvH4_5g21700 FvH4_6g22211
malus_domestica	MD05G1305700.v1.1 MD05G1305800.v1.1 MD05G1305900.v1.1 MD05G1306400.v1.1
prunus_persica	Prupe.4G056200_v2.0.a1 Prupe.4G056200_v2.0.a1 Prupe.4G056600_v2.0.a1 Prupe.4G056600_v2.0.a1
pyrus_communis	pycom05g28300 pycom05g28310 pycom05g28350 pycom10g23860
rosa_chinensis	RchiOBHm_Chr5g0009861 RchiOBHm_Chr5g0009881 RchiOBHm_Chr5g0009931 RchiOBHm_Chr5g0009951 RchiOBHm_Chr5g0009981 RchiOBHm_Chr5g0055531 RchiOBHm_Chr5g0055541
rosa_laevigata	RLG00000015779 RLG00000031705 RLG00000031706 RLG00000031711 RLG00000031713 RLG00000031715 RLG00000031716 RLG00000034996
rosa_multiflora	Rmu_co8083594.1_g000001 Rmu_co8206032.1_g000001 Rmu_co8356045.1_g000001 Rmu_sc0000773.1_g000002 Rmu_sc0001709.1_g000001 Rmu_sc0001709.1_g000007 Rmu_sc0001709.1_g000012 Rmu_sc0001709.1_g000013 Rmu_sc0001709.1_g000018 Rmu_sc0001709.1_g000019 Rmu_sc0002275.1_g000004 Rmu_sc0003071.1_g000002 Rmu_sc0003071.1_g000003 Rmu_sc0003630.1_g000014 Rmu_sc0003630.1_g000017 Rmu_sc0003630.1_g000043 Rmu_sc0003630.1_g000044 Rmu_sc0004626.1_g000007
rosa_roxburghii	Rroxscaffold_1G00024770 Rroxscaffold_1G00066180 Rroxscaffold_1G00066200 Rroxscaffold_1G00066210 Rroxscaffold_1G00066240 Rroxscaffold_1G00066250 Rroxscaffold_1G00066320 Rroxscaffold_1G00066340 Rroxscaffold_3G00234120
rosa_rugosa	Rorug04G0446400 Rorug04G0446500 Rorug04G0446600 Rorug04G0447000 Rorug04G0447100 Rorug04G0447200 Rorug04G0447300 Rorug04G0447900 Rorug04G0448200
rosa_samantha	Rh2CG021600 Rh2DG021300 Rh4AG195700 Rh4BG194700 Rh5AG077900 Rh5AG078100 Rh5AG078600 Rh5AG078700 Rh5AG078900 Rh5AG079000 Rh5AG079100 Rh5AG363100 Rh5CG085300 Rh5CG085900 Rh5CG086000 Rh5CG086200 Rh5CG086400 Rh5CG086500 Rh5CG397300 Rh5DG073400 Rh5DG074000 Rh5DG074100 Rh5DG074300 Rh5DG074500 Rh5DG074600 Rh5DG129000 Rh5DG129400 Rh5DG388500
rosa_wichuraiana	Rw2G001690 Rw5G007180 Rw5G007190 Rw5G007230 Rw5G007240 Rw5G007250 Rw5G007320 Rw5G034180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	422
AccIII	TCCGGA	1 cut(s)	2692
AclI	AACGTT	1 cut(s)	129
AclWI	GGATC	3 cut(s)	430, 1159, 1172
AcoI	YGGCCR	1 cut(s)	341
AcsI	RAATTY	8 cut(s)	935, 978, 1373, 1440, 1601, 2072, 2374, 2604
AcuI	CTGAAG	4 cut(s)	309, 642, 1351, 2212
AfaI	GTAC	7 cut(s)	315, 1057, 1297, 1417, 1644, 1810, 2048
AfiI	CCNNNNNNNGG	4 cut(s)	1619, 1620, 1781, 2663
AgeI	ACCGGT	2 cut(s)	1715, 2017
AhlI	ACTAGT	1 cut(s)	836
AjnI	CCWGG	4 cut(s)	973, 1279, 1351, 2276
AjuI	GAANNNNNNNTTGG	2 cut(s)	1210, 1242
Alw21I	GWGCWC	4 cut(s)	415, 880, 2369, 2620
Alw26I	GTCTC	5 cut(s)	243, 643, 723, 798, 1150
Alw44I	GTGCAC	1 cut(s)	411
AlwI	GGATC	3 cut(s)	430, 1159, 1172
AlwNI	CAGNNNCTG	1 cut(s)	665
Ama87I	CYCGRG	2 cut(s)	720, 1878
Aor13HI	TCCGGA	1 cut(s)	2692
AoxI	GGCC	6 cut(s)	341, 449, 1234, 1277, 2292, 2422
ApaLI	GTGCAC	1 cut(s)	411
ApeKI	GCWGC	6 cut(s)	101, 179, 194, 284, 515, 2150
ApoI	RAATTY	8 cut(s)	935, 978, 1373, 1440, 1601, 2072, 2374, 2604
AseI	ATTAAT	2 cut(s)	1008, 1727
AsiGI	ACCGGT	2 cut(s)	1715, 2017
Asp700I	GAANNNNTTC	2 cut(s)	935, 1688
AspS9I	GGNCC	2 cut(s)	1787, 1915
AsuHPI	GGTGA	7 cut(s)	553, 1145, 1574, 1705, 1816, 1948, 2667
AvaI	CYCGRG	2 cut(s)	720, 1878
AvaII	GGWCC	2 cut(s)	1787, 1915
BaeGI	GKGCMC	2 cut(s)	415, 1766
BaeI	ACNNNNGTAYC	2 cut(s)	1800, 1833
BalI	TGGCCA	1 cut(s)	343
BamHI	GGATCC	1 cut(s)	1164
BanII	GRGCYC	2 cut(s)	880, 2620
BarI	GAAGNNNNNNTAC	2 cut(s)	297, 329
BbsI	GAAGAC	2 cut(s)	48, 558
Bbv12I	GWGCWC	4 cut(s)	415, 880, 2369, 2620
BbvI	GCAGC	6 cut(s)	113, 166, 206, 271, 502, 2137
BccI	CCATC	8 cut(s)	441, 470, 781, 1175, 1302, 1418, 1774, 2024
BciT130I	CCWGG	4 cut(s)	975, 1281, 1353, 2278
BclI	TGATCA	2 cut(s)	364, 2091
BcoDI	GTCTC	5 cut(s)	243, 643, 723, 798, 1150
BcuI	ACTAGT	1 cut(s)	836
BfmI	CTRYAG	2 cut(s)	898, 1864
BglII	AGATCT	1 cut(s)	2596
BisI	GCNGC	6 cut(s)	102, 180, 195, 285, 516, 2151
BlsI	GCNGC	6 cut(s)	103, 181, 196, 286, 517, 2152
BmcAI	AGTACT	1 cut(s)	1057
Bme1390I	CCNGG	4 cut(s)	975, 1281, 1353, 2278
Bme18I	GGWCC	2 cut(s)	1787, 1915
BmeT110I	CYCGRG	2 cut(s)	720, 1878
BmgT120I	GGNCC	2 cut(s)	1787, 1915
BmiI	GGNNCC	6 cut(s)	1166, 1286, 1350, 1507, 1720, 1754
BmrFI	CCNGG	4 cut(s)	975, 1281, 1353, 2278
BmsI	GCATC	3 cut(s)	601, 640, 1540
BpiI	GAAGAC	2 cut(s)	48, 558
BplI	GAGNNNNNCTC	2 cut(s)	2351, 2383
BpmI	CTGGAG	1 cut(s)	2337
BpuEI	CTTGAG	4 cut(s)	369, 837, 1909, 2422
BsaAI	YACGTR	1 cut(s)	464
BsaJI	CCNNGG	5 cut(s)	183, 1237, 1280, 2277, 2657
BsaWI	WCCGGW	5 cut(s)	1715, 2017, 2438, 2692, 2704
Bsc4I	CCNNNNNNNGG	4 cut(s)	1619, 1620, 1781, 2663
Bse118I	RCCGGY	3 cut(s)	394, 1715, 2017
Bse1I	ACTGG	4 cut(s)	471, 1050, 1524, 2738
Bse3DI	GCAATG	2 cut(s)	498, 1320
BseAI	TCCGGA	1 cut(s)	2692
BseBI	CCWGG	4 cut(s)	975, 1281, 1353, 2278
BseDI	CCNNGG	5 cut(s)	183, 1237, 1280, 2277, 2657
BseGI	GGATG	5 cut(s)	1313, 1680, 1989, 2035, 2490
BseLI	CCNNNNNNNGG	4 cut(s)	1619, 1620, 1781, 2663
BseMI	GCAATG	2 cut(s)	498, 1320
BseMII	CTCAG	6 cut(s)	267, 588, 657, 822, 1151, 1622
BseNI	ACTGG	4 cut(s)	471, 1050, 1524, 2738
BseRI	GAGGAG	6 cut(s)	840, 1676, 1822, 2372, 2375, 2503
BseSI	GKGCMC	2 cut(s)	415, 1766
BseXI	GCAGC	6 cut(s)	113, 166, 206, 271, 502, 2137
BshFI	GGCC	6 cut(s)	343, 451, 1236, 1279, 2294, 2424
BshTI	ACCGGT	2 cut(s)	1715, 2017
BsiHKAI	GWGCWC	4 cut(s)	415, 880, 2369, 2620
BsiHKCI	CYCGRG	2 cut(s)	720, 1878
BsiSI	CCGG	6 cut(s)	395, 1716, 2018, 2439, 2693, 2705
BslFI	GGGAC	1 cut(s)	804
BslI	CCNNNNNNNGG	4 cut(s)	1619, 1620, 1781, 2663
BsmAI	GTCTC	5 cut(s)	243, 643, 723, 798, 1150
BsmFI	GGGAC	1 cut(s)	804
BsmI	GAATGC	3 cut(s)	1041, 1434, 2735
BsnI	GGCC	6 cut(s)	343, 451, 1236, 1279, 2294, 2424
BsoBI	CYCGRG	2 cut(s)	720, 1878
Bsp1286I	GDGCHC	5 cut(s)	415, 880, 1766, 2369, 2620
Bsp13I	TCCGGA	1 cut(s)	2692
Bsp1407I	TGTACA	1 cut(s)	2046
Bsp143I	GATC	6 cut(s)	145, 364, 435, 1164, 2091, 2596
Bsp19I	CCATGG	1 cut(s)	183
BspANI	GGCC	6 cut(s)	343, 451, 1236, 1279, 2294, 2424
BspCNI	CTCAG	6 cut(s)	266, 589, 658, 821, 1152, 1621
BspEI	TCCGGA	1 cut(s)	2692
BspHI	TCATGA	2 cut(s)	226, 2184
BspLI	GGNNCC	6 cut(s)	1166, 1286, 1350, 1507, 1720, 1754
BspPI	GGATC	3 cut(s)	430, 1159, 1172
BspQI	GCTCTTC	1 cut(s)	609
BsrDI	GCAATG	2 cut(s)	498, 1320
BsrFI	RCCGGY	3 cut(s)	394, 1715, 2017
BsrGI	TGTACA	1 cut(s)	2046
BsrI	ACTGG	4 cut(s)	471, 1050, 1524, 2738
BssAI	RCCGGY	3 cut(s)	394, 1715, 2017
BssECI	CCNNGG	5 cut(s)	183, 1237, 1280, 2277, 2657
BssMI	GATC	6 cut(s)	145, 364, 435, 1164, 2091, 2596
BssT1I	CCWWGG	3 cut(s)	183, 1237, 2657
Bst2UI	CCWGG	4 cut(s)	975, 1281, 1353, 2278
Bst4CI	ACNGT	7 cut(s)	297, 560, 757, 902, 1143, 1573, 2566
Bst6I	CTCTTC	5 cut(s)	609, 684, 993, 1482, 1951
BstAPI	GCANNNNNTGC	1 cut(s)	2522
BstAUI	TGTACA	1 cut(s)	2046
BstBAI	YACGTR	1 cut(s)	464
BstC8I	GCNNGC	6 cut(s)	581, 846, 1970, 2518, 2718, 2735
BstDEI	CTNAG	9 cut(s)	253, 597, 666, 780, 808, 1160, 1289, 1608, 2289
BstDSI	CCRYGG	1 cut(s)	183
BstEII	GGTNACC	1 cut(s)	1711
BstF5I	GGATG	5 cut(s)	1313, 1680, 1989, 2035, 2490
BstKTI	GATC	6 cut(s)	148, 367, 438, 1167, 2094, 2599
BstMAI	GTCTC	5 cut(s)	243, 643, 723, 798, 1150
BstMBI	GATC	6 cut(s)	145, 364, 435, 1164, 2091, 2596
BstMWI	GCNNNNNNNGC	4 cut(s)	281, 2412, 2421, 2522
BstNI	CCWGG	4 cut(s)	975, 1281, 1353, 2278
BstNSI	RCATGY	1 cut(s)	2737
BstPI	GGTNACC	1 cut(s)	1711
BstSCI	CCNGG	4 cut(s)	973, 1279, 1351, 2276
BstSFI	CTRYAG	2 cut(s)	898, 1864
BstSLI	GKGCMC	2 cut(s)	415, 1766
BstSNI	TACGTA	1 cut(s)	464
BstV1I	GCAGC	6 cut(s)	113, 166, 206, 271, 502, 2137
BstV2I	GAAGAC	2 cut(s)	48, 558
BstX2I	RGATCY	2 cut(s)	1164, 2596
BstYI	RGATCY	2 cut(s)	1164, 2596
BsuRI	GGCC	6 cut(s)	343, 451, 1236, 1279, 2294, 2424
BtgI	CCRYGG	1 cut(s)	183
BtsCI	GGATG	5 cut(s)	1313, 1680, 1989, 2035, 2490
BtsI	GCAGTG	2 cut(s)	1932, 2527
BtsIMutI	CAGTG	8 cut(s)	124, 431, 478, 666, 762, 907, 1932, 2527
Cac8I	GCNNGC	6 cut(s)	581, 846, 1970, 2518, 2718, 2735
CaiI	CAGNNNCTG	1 cut(s)	665
CciI	TCATGA	2 cut(s)	226, 2184
Cfr10I	RCCGGY	3 cut(s)	394, 1715, 2017
Cfr13I	GGNCC	2 cut(s)	1787, 1915
Csp6I	GTAC	7 cut(s)	314, 1056, 1296, 1416, 1643, 1809, 2047
CspAI	ACCGGT	2 cut(s)	1715, 2017
CspCI	CAANNNNNGTGG	2 cut(s)	2324, 2359
CviAII	CATG	9 cut(s)	184, 227, 331, 368, 1671, 1695, 2056, 2185, 2734
CviQI	GTAC	7 cut(s)	314, 1056, 1296, 1416, 1643, 1809, 2047
DdeI	CTNAG	9 cut(s)	253, 597, 666, 780, 808, 1160, 1289, 1608, 2289
DpnI	GATC	6 cut(s)	147, 366, 437, 1166, 2093, 2598
DpnII	GATC	6 cut(s)	145, 364, 435, 1164, 2091, 2596
DraI	TTTAAA	1 cut(s)	2587
EaeI	YGGCCR	1 cut(s)	341
Eam1104I	CTCTTC	5 cut(s)	609, 684, 993, 1482, 1951
EarI	CTCTTC	5 cut(s)	609, 684, 993, 1482, 1951
Ecl136II	GAGCTC	2 cut(s)	878, 2618
Eco105I	TACGTA	1 cut(s)	464
Eco130I	CCWWGG	3 cut(s)	183, 1237, 2657
Eco147I	AGGCCT	1 cut(s)	1236
Eco24I	GRGCYC	2 cut(s)	880, 2620
Eco47I	GGWCC	2 cut(s)	1787, 1915
Eco53kI	GAGCTC	2 cut(s)	878, 2618
Eco57I	CTGAAG	4 cut(s)	309, 642, 1351, 2212
Eco88I	CYCGRG	2 cut(s)	720, 1878
Eco91I	GGTNACC	1 cut(s)	1711
EcoICRI	GAGCTC	2 cut(s)	878, 2618
EcoO65I	GGTNACC	1 cut(s)	1711
EcoRI	GAATTC	3 cut(s)	935, 1601, 2374
EcoRII	CCWGG	4 cut(s)	973, 1279, 1351, 2276
EcoT14I	CCWWGG	3 cut(s)	183, 1237, 2657
EcoT22I	ATGCAT	2 cut(s)	500, 2735
EcoT38I	GRGCYC	2 cut(s)	880, 2620
ErhI	CCWWGG	3 cut(s)	183, 1237, 2657
FaeI	CATG	9 cut(s)	187, 230, 334, 371, 1674, 1698, 2059, 2188, 2737
FaqI	GGGAC	1 cut(s)	804
FatI	CATG	9 cut(s)	183, 226, 330, 367, 1670, 1694, 2055, 2184, 2733
FbaI	TGATCA	2 cut(s)	364, 2091
Fnu4HI	GCNGC	6 cut(s)	102, 180, 195, 285, 516, 2151
FokI	GGATG	5 cut(s)	1320, 1687, 1976, 2042, 2497
FriOI	GRGCYC	2 cut(s)	880, 2620
Fsp4HI	GCNGC	6 cut(s)	102, 180, 195, 285, 516, 2151
GluI	GCNGC	6 cut(s)	102, 180, 195, 285, 516, 2151
GsuI	CTGGAG	1 cut(s)	2337
HaeIII	GGCC	6 cut(s)	343, 451, 1236, 1279, 2294, 2424
HapII	CCGG	6 cut(s)	395, 1716, 2018, 2439, 2693, 2705
Hin1II	CATG	9 cut(s)	187, 230, 334, 371, 1674, 1698, 2059, 2188, 2737
HincII	GTYRAC	1 cut(s)	2349
HindII	GTYRAC	1 cut(s)	2349
HindIII	AAGCTT	3 cut(s)	202, 701, 2398
HinfI	GANTC	8 cut(s)	251, 806, 885, 905, 1148, 1798, 2036, 2350
HpaII	CCGG	6 cut(s)	395, 1716, 2018, 2439, 2693, 2705
HphI	GGTGA	7 cut(s)	553, 1145, 1574, 1705, 1816, 1948, 2667
Hpy166II	GTNNAC	4 cut(s)	413, 1824, 2047, 2349
Hpy8I	GTNNAC	4 cut(s)	413, 1824, 2047, 2349
Hpy99I	CGWCG	1 cut(s)	560
HpyCH4III	ACNGT	7 cut(s)	297, 560, 757, 902, 1143, 1573, 2566
HpyCH4IV	ACGT	5 cut(s)	129, 463, 1294, 1544, 2178
HpyF10VI	GCNNNNNNNGC	4 cut(s)	281, 2412, 2421, 2522
HpyF3I	CTNAG	9 cut(s)	253, 597, 666, 780, 808, 1160, 1289, 1608, 2289
HpySE526I	ACGT	5 cut(s)	129, 463, 1294, 1544, 2178
Hsp92II	CATG	9 cut(s)	187, 230, 334, 371, 1674, 1698, 2059, 2188, 2737
Kpn2I	TCCGGA	1 cut(s)	2692
Ksp22I	TGATCA	2 cut(s)	364, 2091
Kzo9I	GATC	6 cut(s)	145, 364, 435, 1164, 2091, 2596
LguI	GCTCTTC	1 cut(s)	609
LmnI	GCTCC	6 cut(s)	134, 853, 875, 2505, 2623, 2695
Lsp1109I	GCAGC	6 cut(s)	113, 166, 206, 271, 502, 2137
LweI	GCATC	3 cut(s)	601, 640, 1540
MaeII	ACGT	5 cut(s)	129, 463, 1294, 1544, 2178
MaeIII	GTNAC	6 cut(s)	121, 857, 1043, 1651, 1711, 1981
MalI	GATC	6 cut(s)	147, 366, 437, 1166, 2093, 2598
MboI	GATC	6 cut(s)	145, 364, 435, 1164, 2091, 2596
MfeI	CAATTG	2 cut(s)	523, 2271
MflI	RGATCY	2 cut(s)	1164, 2596
MhlI	GDGCHC	5 cut(s)	415, 880, 1766, 2369, 2620
MlsI	TGGCCA	1 cut(s)	343
MluNI	TGGCCA	1 cut(s)	343
MlyI	GAGTC	4 cut(s)	245, 800, 914, 2344
MmeI	TCCRAC	5 cut(s)	112, 243, 463, 1125, 2645
Mox20I	TGGCCA	1 cut(s)	343
Mph1103I	ATGCAT	2 cut(s)	500, 2735
MroI	TCCGGA	1 cut(s)	2692
MroXI	GAANNNNTTC	2 cut(s)	935, 1688
MscI	TGGCCA	1 cut(s)	343
MslI	CAYNNNNRTG	1 cut(s)	1994
Msp20I	TGGCCA	1 cut(s)	343
MspA1I	CMGCKG	2 cut(s)	197, 2150
MspI	CCGG	6 cut(s)	395, 1716, 2018, 2439, 2693, 2705
MspR9I	CCNGG	4 cut(s)	975, 1281, 1353, 2278
MunI	CAATTG	2 cut(s)	523, 2271
Mva1269I	GAATGC	3 cut(s)	1041, 1434, 2735
MvaI	CCWGG	4 cut(s)	975, 1281, 1353, 2278
MwoI	GCNNNNNNNGC	4 cut(s)	281, 2412, 2421, 2522
NcoI	CCATGG	1 cut(s)	183
NdeII	GATC	6 cut(s)	145, 364, 435, 1164, 2091, 2596
NlaIII	CATG	9 cut(s)	187, 230, 334, 371, 1674, 1698, 2059, 2188, 2737
NlaIV	GGNNCC	6 cut(s)	1166, 1286, 1350, 1507, 1720, 1754
NmuCI	GTSAC	2 cut(s)	121, 1711
NsiI	ATGCAT	2 cut(s)	500, 2735
NspI	RCATGY	1 cut(s)	2737
PaeI	GCATGC	1 cut(s)	2737
PaeR7I	CTCGAG	1 cut(s)	1878
PagI	TCATGA	2 cut(s)	226, 2184
PceI	AGGCCT	1 cut(s)	1236
PciSI	GCTCTTC	1 cut(s)	609
PctI	GAATGC	3 cut(s)	1041, 1434, 2735
PdmI	GAANNNNTTC	2 cut(s)	935, 1688
PfeI	GAWTC	4 cut(s)	885, 1148, 1798, 2036
PfoI	TCCNGGA	1 cut(s)	1351
PinAI	ACCGGT	2 cut(s)	1715, 2017
PkrI	GCNGC	6 cut(s)	103, 181, 196, 286, 517, 2152
PleI	GAGTC	4 cut(s)	245, 800, 913, 2344
PpsI	GAGTC	4 cut(s)	245, 800, 913, 2344
Ppu21I	YACGTR	1 cut(s)	464
PshBI	ATTAAT	2 cut(s)	1008, 1727
PsiI	TTATAA	1 cut(s)	422
Psp124BI	GAGCTC	2 cut(s)	880, 2620
Psp1406I	AACGTT	1 cut(s)	129
Psp6I	CCWGG	4 cut(s)	973, 1279, 1351, 2276
PspEI	GGTNACC	1 cut(s)	1711
PspGI	CCWGG	4 cut(s)	973, 1279, 1351, 2276
PspN4I	GGNNCC	6 cut(s)	1166, 1286, 1350, 1507, 1720, 1754
PspPI	GGNCC	2 cut(s)	1787, 1915
PspXI	VCTCGAGB	1 cut(s)	1878
PstNI	CAGNNNCTG	1 cut(s)	665
PsuI	RGATCY	2 cut(s)	1164, 2596
PvuII	CAGCTG	2 cut(s)	197, 2150
RsaI	GTAC	7 cut(s)	315, 1057, 1297, 1417, 1644, 1810, 2048
RsaNI	GTAC	7 cut(s)	314, 1056, 1296, 1416, 1643, 1809, 2047
RseI	CAYNNNNRTG	1 cut(s)	1994
SacI	GAGCTC	2 cut(s)	880, 2620
SapI	GCTCTTC	1 cut(s)	609
SatI	GCNGC	6 cut(s)	102, 180, 195, 285, 516, 2151
Sau3AI	GATC	6 cut(s)	145, 364, 435, 1164, 2091, 2596
Sau96I	GGNCC	2 cut(s)	1787, 1915
ScaI	AGTACT	1 cut(s)	1057
SchI	GAGTC	4 cut(s)	245, 800, 914, 2344
ScrFI	CCNGG	4 cut(s)	975, 1281, 1353, 2278
SduI	GDGCHC	5 cut(s)	415, 880, 1766, 2369, 2620
SfaNI	GCATC	3 cut(s)	601, 640, 1540
SfcI	CTRYAG	2 cut(s)	898, 1864
Sfr274I	CTCGAG	1 cut(s)	1878
SinI	GGWCC	2 cut(s)	1787, 1915
SlaI	CTCGAG	1 cut(s)	1878
SmiMI	CAYNNNNRTG	1 cut(s)	1994
SmlI	CTYRAG	5 cut(s)	384, 852, 1878, 1924, 2401
SmoI	CTYRAG	5 cut(s)	384, 852, 1878, 1924, 2401
SnaBI	TACGTA	1 cut(s)	464
SpeI	ACTAGT	1 cut(s)	836
SphI	GCATGC	1 cut(s)	2737
SseBI	AGGCCT	1 cut(s)	1236
SspI	AATATT	1 cut(s)	2551
SstI	GAGCTC	2 cut(s)	880, 2620
StuI	AGGCCT	1 cut(s)	1236
StyD4I	CCNGG	4 cut(s)	973, 1279, 1351, 2276
StyI	CCWWGG	3 cut(s)	183, 1237, 2657
TaaI	ACNGT	7 cut(s)	297, 560, 757, 902, 1143, 1573, 2566
TaiI	ACGT	5 cut(s)	132, 466, 1297, 1547, 2181
TaqI	TCGA	5 cut(s)	247, 991, 1179, 1879, 2593
TaqII	GACCGA	1 cut(s)	1599
TatI	WGTACW	2 cut(s)	1055, 2046
TfiI	GAWTC	4 cut(s)	885, 1148, 1798, 2036
TscAI	CASTG	8 cut(s)	124, 438, 478, 666, 762, 907, 1939, 2527
TseFI	GTSAC	2 cut(s)	121, 1711
TseI	GCWGC	6 cut(s)	101, 179, 194, 284, 515, 2150
Tsp45I	GTSAC	2 cut(s)	121, 1711
TspGWI	ACGGA	2 cut(s)	1470, 2331
TspRI	CASTG	8 cut(s)	124, 438, 478, 666, 762, 907, 1939, 2527
VneI	GTGCAC	1 cut(s)	411
VpaK11BI	GGWCC	2 cut(s)	1787, 1915
VspI	ATTAAT	2 cut(s)	1008, 1727
XapI	RAATTY	8 cut(s)	935, 978, 1373, 1440, 1601, 2072, 2374, 2604
XceI	RCATGY	1 cut(s)	2737
XhoI	CTCGAG	1 cut(s)	1878
XmnI	GAANNNNTTC	2 cut(s)	935, 1688
ZrmI	AGTACT	1 cut(s)	1057
Zsp2I	ATGCAT	2 cut(s)	500, 2735

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.