Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0000350

Physical Location & Seq

Genomic Coordinates Forward (+)

6700217 .. 6703482

Gene Structure 3266 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG078100.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2586 bp

ATGTCTCTTTGTTTTACACTCCACACAAATAGGCTCTTGGTGCCTCTTCTCATCACCTGTTTGATAATTTGTCTCGAGGGAGTTGCAGTTCAAAATGAAAAAATTGTCACTAACGTTGGAGCAATCATTAATGCTGATTCCCGTATCGGCAAAGAACAGAAAGCTGCCATGGAAATTGCAGCTGAAAGCTTCAACAACAAGTCCGGCAACACTCGTGAACTAATCCTTCATTTTCAAGACTCCGGGGGAGATCCCTTTCTTGCTGCTTCTGAGGTTGAAGAGTTGATAAAGGAAAAGAAAGTAGAAGTGATAGTTGGCATGGAGACTTGGCAGGAAGCAGCTCTAGTAGCTGATCATGTGGGAAACCAATCTAAAAAAATTCCGGTAATTTCATTTGCAGCACCAGCCATAACCGTGCCACTAATGCAACAGCGTTGGCCATTTCTGATACGAATGGCAAGCAATGGATCTGCTCAGATGAAATGCATTGCAGATATAGTTTCTGCATACAAGTGGAAAAGGGTTGTTGTGATATACGAAGACGACGGTTATGGTGGTCAAGTTGGGTTGCTAGCTCTTTTATCTGAGGCTCTTCAAGATGCAAATGCAAAGATTGAGAATCATTTGGTTCTTCCGAGACTAAATAATTCTTCGATGGCTAAGCCTAATTGGGATGAAGTAGAAGAGCTGTTGAAGCTTCCCACCTTTCAGTCTCGGGTGTTTGTTGTTCTTCAATCATCTTTACCAACAGTGACTAATCTGTTCAGCGTGGCTAGGAAGATGGGACTTGTAGGGAGAGAGTCGGCTTGGATAATCACAGAATGTATCACCAGTTTGCTTGACCCTCAAGGTGACGACTCTATGGAAGGAACTCTGGGAATCAAGACCGATTACGCCACTGAGTCTAGTTCTTATGCTGAATTCCAGAAGGAATTCCAAGCTAAGTATGCAGAAGAAAATAACTTCAAGCCAGGAATTTATGCTCTTCGAGCATACGACACTATTACTGTCATTTCAAAGGCCTTAGAAAGGATGCCTAATAACACCACTAGTACAAGCCTTCAGAATATGATAACTACATTGTTGAGCAATTACAAGGGTTTAAGTGGGAATATGCACTTGAAAGAAGGTGCAGCACTGTTGGATTCTCTGAGATTCAGGATCATAAATGTTGTGGATGGGAAGAGGAACAAAGAGTTAAACTTTTGGTCACCAGAGGTTGGGTTCTCCGAAGGCCTAGGCTGGGCAGCCTCACACGAGAACCTCACACGTGCGCCTCAAGGCTGGGGAATGCCTCCTGCTGGGAAGCCAATGAAATTTGGGGTTCCAGCTGGTAACAAGCACTTCGGTAAATTTGTAAAGGTTGAACCCAGAAACAATTCATCATATGACTTCTCATATGACGGTTTCTCCATTAGAATTTTCTACACGGTACTAACTCATTTGAATTATCATCTACCATTTGAATTTGTACCCTTTAACGGTTCTTACGATGATTTGGTAGAAGGAGTTTATAACAAGACTTTTGATGCTGCTGTTGGTGATTTCACTATATTACCTGAGCGAATGGAGAAAGTGGAATTCACTCAGCCGTATATGAAGTCTGGTTTGTCAATGATAGTCGTAGAAAAATACGATGAATCAACATGGATGTTTGTGAGACCTTTCACATGGAAAATATGGGTGGTGAGTGGTGCCATCTTAATCTACACTAGTTTAGTAGTATGGTTCCTGGAGCGCTCAAGAAATCCAGAATTTGATGGTTCATTGAAGAATCAGATTGGCACAGCAACTTATATCACTTTCTCCTCCTTGTTCTTTGCTCACAAAGAAAAAGTATATACCAACTTTGCTCGGGTAGTATTTCTTCTGTGGCTATTTATTGTATTGATCTTGAGCTCAAGCTACACTGCTAGTCTTTCTTCCATGCTTACCACCCAACGACCGACCTTAAATGTAACAGCTGAAATGTTAAACAAGACTGGTGCAAAAGTTGGTTGGGATGGAGGTCCATTTGTCTCAAAGTATCTGTCAGATACCTATAAATTCGATGCTAACAACAGAATCAATGTAAGCAGCAAAAACAGCTACACTGATGCATTCAAAAGCAAAAATATAACTGCTATGTTTCTTGAACTCCCATATGCTCAAGTCTACATGAACGAGCTCTGTGAGGGATATACTTCAACCACACAACCCTACGAATTTGGAGGATTTAGCTTTATATTCCAGAAAGGCTCTCCAATAGCTAGGGATTTCTCTAAGGCCATTCTAGAGCTATTGGAGAATGGATATATCAAGCAACTTCAAAATGAATTATTGACTCCCGAGAAGCAGTGTCCAAACACTAATAGTAGACCAAAAAGCTTGAGCCTTAATAGCTTTTCGGGTCTCTACGTAATATGCGGTGCTACTTCCACCCTGTGTCTTCTACTATCTTTTACCCCATGTGTGAGGAAGTCTAAGAAGCAAGAAAAGCACGAAGGCAATGCAGGTCCAAGTGATGAATGTCTCCTTCGATCCCAACACTCCAACAGTCCGCAGGCCTTCACATCCGTAGAAATCGAATGCGTGCCACAGAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

861

Amino Acids

96.31

Weight (kDa)

6.38

Isoelectric Point (pI)

40.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	49 - 370	1.8e-19	Periplasmic binding protein
ANF_receptor	PF01094	54 - 392	2.3e-42	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	462 - 541	1.3e-07	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	466 - 772	9.1e-13	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	557 - 804	3.2e-25	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000350)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g04591 FvH4_1g04600 FvH4_3g08210 FvH4_3g08210 FvH4_3g08211 FvH4_3g32960 FvH4_3g32961 FvH4_5g21700 FvH4_6g22211
malus_domestica	MD05G1305700.v1.1 MD05G1305800.v1.1 MD05G1305900.v1.1 MD05G1306400.v1.1
prunus_persica	Prupe.4G056200_v2.0.a1 Prupe.4G056200_v2.0.a1 Prupe.4G056600_v2.0.a1 Prupe.4G056600_v2.0.a1
pyrus_communis	pycom05g28300 pycom05g28310 pycom05g28350 pycom10g23860
rosa_chinensis	RchiOBHm_Chr5g0009861 RchiOBHm_Chr5g0009881 RchiOBHm_Chr5g0009931 RchiOBHm_Chr5g0009951 RchiOBHm_Chr5g0009981 RchiOBHm_Chr5g0055531 RchiOBHm_Chr5g0055541
rosa_laevigata	RLG00000015779 RLG00000031705 RLG00000031706 RLG00000031711 RLG00000031713 RLG00000031715 RLG00000031716 RLG00000034996
rosa_multiflora	Rmu_co8083594.1_g000001 Rmu_co8206032.1_g000001 Rmu_co8356045.1_g000001 Rmu_sc0000773.1_g000002 Rmu_sc0001709.1_g000001 Rmu_sc0001709.1_g000007 Rmu_sc0001709.1_g000012 Rmu_sc0001709.1_g000013 Rmu_sc0001709.1_g000018 Rmu_sc0001709.1_g000019 Rmu_sc0002275.1_g000004 Rmu_sc0003071.1_g000002 Rmu_sc0003071.1_g000003 Rmu_sc0003630.1_g000014 Rmu_sc0003630.1_g000017 Rmu_sc0003630.1_g000043 Rmu_sc0003630.1_g000044 Rmu_sc0004626.1_g000007
rosa_roxburghii	Rroxscaffold_1G00024770 Rroxscaffold_1G00066180 Rroxscaffold_1G00066200 Rroxscaffold_1G00066210 Rroxscaffold_1G00066240 Rroxscaffold_1G00066250 Rroxscaffold_1G00066320 Rroxscaffold_1G00066340 Rroxscaffold_3G00234120
rosa_rugosa	Rorug04G0446400 Rorug04G0446500 Rorug04G0446600 Rorug04G0447000 Rorug04G0447100 Rorug04G0447200 Rorug04G0447300 Rorug04G0447900 Rorug04G0448200
rosa_samantha	Rh2CG021600 Rh2DG021300 Rh4AG195700 Rh4BG194700 Rh5AG077900 Rh5AG078100 Rh5AG078600 Rh5AG078700 Rh5AG078900 Rh5AG079000 Rh5AG079100 Rh5AG363100 Rh5CG085300 Rh5CG085900 Rh5CG086000 Rh5CG086200 Rh5CG086400 Rh5CG086500 Rh5CG397300 Rh5DG073400 Rh5DG074000 Rh5DG074100 Rh5DG074300 Rh5DG074500 Rh5DG074600 Rh5DG129000 Rh5DG129400 Rh5DG388500
rosa_wichuraiana	Rw2G001690 Rw5G007180 Rw5G007190 Rw5G007230 Rw5G007240 Rw5G007250 Rw5G007320 Rw5G034180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1515
Acc36I	ACCTGC	1 cut(s)	2483
AccB1I	GGYRCC	2 cut(s)	40, 1694
AccB7I	CCANNNNNTGG	1 cut(s)	1220
AccI	GTMKAC	2 cut(s)	2154, 2356
AciI	CCGC	2 cut(s)	2406, 2540
AclI	AACGTT	1 cut(s)	114
AclWI	GGATC	4 cut(s)	245, 475, 1169, 2514
AcoI	YGGCCR	1 cut(s)	437
AcuI	CTGAAG	1 cut(s)	1046
AcvI	CACGTG	1 cut(s)	1271
AdeI	CACNNNGTG	1 cut(s)	2424
AfaI	GTAC	3 cut(s)	1054, 1434, 1473
AfeI	AGCGCT	1 cut(s)	1739
AfiI	CCNNNNNNNGG	5 cut(s)	1029, 1220, 1243, 1301, 1481
AflIII	ACRYGT	1 cut(s)	1268
AhlI	ACTAGT	2 cut(s)	1049, 1712
AjnI	CCWGG	2 cut(s)	970, 1731
AjuI	GAANNNNNNNTTGG	2 cut(s)	297, 329
AloI	GAACNNNNNNTCC	2 cut(s)	72, 104
Alw21I	GWGCWC	2 cut(s)	1901, 2169
Alw26I	GTCTC	9 cut(s)	9, 77, 317, 631, 717, 1654, 2023, 2396, 2516
AlwI	GGATC	4 cut(s)	245, 475, 1169, 2514
Ama87I	CYCGRG	4 cut(s)	74, 714, 1854, 2327
Aor51HI	AGCGCT	1 cut(s)	1739
AoxI	GGCC	5 cut(s)	437, 1020, 1234, 2265, 2544
ApeKI	GCWGC	9 cut(s)	164, 179, 263, 338, 398, 1133, 1247, 1532, 2076
ArsI	GACNNNNNNTTYG	2 cut(s)	2015, 2047
AseI	ATTAAT	1 cut(s)	129
Asp700I	GAANNNNTTC	2 cut(s)	932, 1378
AspA2I	CCTAGG	1 cut(s)	1237
AspLEI	GCGC	2 cut(s)	1276, 1740
AspS9I	GGNCC	2 cut(s)	2009, 2495
AsuC2I	CCSGG	1 cut(s)	244
AsuHPI	GGTGA	6 cut(s)	46, 820, 863, 1203, 1553, 1699
AsuNHI	GCTAGC	1 cut(s)	571
AvaI	CYCGRG	4 cut(s)	74, 714, 1854, 2327
AvaII	GGWCC	2 cut(s)	2009, 2495
AvrII	CCTAGG	1 cut(s)	1237
BalI	TGGCCA	1 cut(s)	439
BanI	GGYRCC	2 cut(s)	40, 1694
BanII	GRGCYC	2 cut(s)	1901, 2169
BarI	GAAGNNNNNNTAC	2 cut(s)	1851, 1883
BauI	CACGAG	2 cut(s)	213, 1256
BbrPI	CACGTG	1 cut(s)	1271
BbsI	GAAGAC	2 cut(s)	546, 2420
Bbv12I	GWGCWC	2 cut(s)	1901, 2169
BbvI	GCAGC	9 cut(s)	151, 191, 250, 350, 410, 1145, 1259, 1519, 2088
BccI	CCATC	6 cut(s)	649, 775, 1172, 1706, 1754, 1997
BceAI	ACGGC	1 cut(s)	1576
BcgI	CGANNNNNNTGC	4 cut(s)	65, 99, 2471, 2505
BciT130I	CCWGG	2 cut(s)	972, 1733
BclI	TGATCA	1 cut(s)	352
BcnI	CCSGG	1 cut(s)	244
BcoDI	GTCTC	9 cut(s)	9, 77, 317, 631, 717, 1654, 2023, 2396, 2516
BcuI	ACTAGT	2 cut(s)	1049, 1712
BfoI	RGCGCY	1 cut(s)	1741
BfuAI	ACCTGC	1 cut(s)	2483
BisI	GCNGC	9 cut(s)	165, 180, 264, 339, 399, 1134, 1248, 1533, 2077
BlnI	CCTAGG	1 cut(s)	1237
BlpI	GCTNAGC	1 cut(s)	660
BlsI	GCNGC	9 cut(s)	166, 181, 265, 340, 400, 1135, 1249, 1534, 2078
Bme1390I	CCNGG	3 cut(s)	244, 972, 1733
Bme18I	GGWCC	2 cut(s)	2009, 2495
BmeT110I	CYCGRG	4 cut(s)	74, 714, 1854, 2327
BmgT120I	GGNCC	2 cut(s)	2009, 2495
BmiI	GGNNCC	4 cut(s)	42, 1326, 1696, 1730
BmrFI	CCNGG	3 cut(s)	244, 972, 1733
BmsI	GCATC	5 cut(s)	589, 1023, 1519, 2041, 2086
BmtI	GCTAGC	1 cut(s)	575
BpiI	GAAGAC	2 cut(s)	546, 2420
BpmI	CTGGAG	1 cut(s)	1754
Bpu10I	CCTNAGC	1 cut(s)	1560
Bpu1102I	GCTNAGC	1 cut(s)	660
BpuEI	CTTGAG	7 cut(s)	831, 1263, 1726, 1885, 1915, 2133, 2389
BpuMI	CCSGG	1 cut(s)	244
BsaAI	YACGTR	2 cut(s)	1271, 2398
BsaI	GGTCTC	2 cut(s)	1654, 2396
BsaJI	CCNNGG	3 cut(s)	168, 243, 1237
BsaWI	WCCGGW	1 cut(s)	382
BsaXI	ACNNNNNCTCC	4 cut(s)	72, 102, 1394, 1424
Bsc4I	CCNNNNNNNGG	5 cut(s)	1029, 1220, 1243, 1301, 1481
Bse1I	ACTGG	2 cut(s)	831, 1987
Bse3DI	GCAATG	3 cut(s)	469, 486, 2494
BseBI	CCWGG	2 cut(s)	972, 1733
BseDI	CCNNGG	3 cut(s)	168, 243, 1237
BseGI	GGATG	6 cut(s)	679, 1038, 1183, 1656, 2008, 2552
BseLI	CCNNNNNNNGG	5 cut(s)	1029, 1220, 1243, 1301, 1481
BseMI	GCAATG	3 cut(s)	469, 486, 2494
BseMII	CTCAG	7 cut(s)	261, 488, 576, 891, 1142, 1551, 1601
BseNI	ACTGG	2 cut(s)	831, 1987
BseRI	GAGGAG	1 cut(s)	1798
BseXI	GCAGC	9 cut(s)	151, 191, 250, 350, 410, 1145, 1259, 1519, 2088
BseYI	CCCAGC	3 cut(s)	1242, 1284, 1301
BsgI	GTGCAG	1 cut(s)	1152
Bsh1285I	CGRYCG	1 cut(s)	1946
BshFI	GGCC	5 cut(s)	439, 1022, 1236, 2267, 2546
BshNI	GGYRCC	2 cut(s)	40, 1694
BsiEI	CGRYCG	1 cut(s)	1946
BsiHKAI	GWGCWC	2 cut(s)	1901, 2169
BsiHKCI	CYCGRG	4 cut(s)	74, 714, 1854, 2327
BsiSI	CCGG	3 cut(s)	204, 243, 383
BslFI	GGGAC	1 cut(s)	798
BslI	CCNNNNNNNGG	5 cut(s)	1029, 1220, 1243, 1301, 1481
BsmAI	GTCTC	9 cut(s)	9, 77, 317, 631, 717, 1654, 2023, 2396, 2516
BsmFI	GGGAC	1 cut(s)	798
BsmI	GAATGC	3 cut(s)	1296, 2099, 2573
BsnI	GGCC	5 cut(s)	439, 1022, 1236, 2267, 2546
Bso31I	GGTCTC	2 cut(s)	1654, 2396
BsoBI	CYCGRG	4 cut(s)	74, 714, 1854, 2327
Bsp1286I	GDGCHC	2 cut(s)	1901, 2169
Bsp143I	GATC	6 cut(s)	250, 352, 467, 1161, 1890, 2519
Bsp1720I	GCTNAGC	1 cut(s)	660
Bsp19I	CCATGG	1 cut(s)	168
BspACI	CCGC	2 cut(s)	2406, 2540
BspANI	GGCC	5 cut(s)	439, 1022, 1236, 2267, 2546
BspCNI	CTCAG	7 cut(s)	262, 487, 577, 892, 1143, 1552, 1600
BspLI	GGNNCC	4 cut(s)	42, 1326, 1696, 1730
BspMI	ACCTGC	1 cut(s)	2483
BspOI	GCTAGC	1 cut(s)	575
BspPI	GGATC	4 cut(s)	245, 475, 1169, 2514
BspQI	GCTCTTC	3 cut(s)	597, 678, 990
BspT107I	GGYRCC	2 cut(s)	40, 1694
BspTNI	GGTCTC	2 cut(s)	1654, 2396
BsrDI	GCAATG	3 cut(s)	469, 486, 2494
BsrI	ACTGG	2 cut(s)	831, 1987
BssECI	CCNNGG	3 cut(s)	168, 243, 1237
BssMI	GATC	6 cut(s)	250, 352, 467, 1161, 1890, 2519
BssSI	CACGAG	2 cut(s)	213, 1256
BssT1I	CCWWGG	2 cut(s)	168, 1237
Bst2BI	CACGAG	2 cut(s)	213, 1256
Bst2UI	CCWGG	2 cut(s)	972, 1733
Bst4CI	ACNGT	9 cut(s)	415, 548, 751, 1009, 1140, 1406, 1432, 1484, 2537
Bst6I	CTCTTC	6 cut(s)	51, 273, 597, 678, 990, 1178
BstBAI	YACGTR	2 cut(s)	1271, 2398
BstC8I	GCNNGC	4 cut(s)	460, 573, 2544, 2573
BstDSI	CCRYGG	1 cut(s)	168
BstEII	GGTNACC	1 cut(s)	1209
BstENI	CCTNNNNNAGG	1 cut(s)	1027
BstF5I	GGATG	6 cut(s)	679, 1038, 1183, 1656, 2008, 2552
BstH2I	RGCGCY	1 cut(s)	1741
BstHHI	GCGC	2 cut(s)	1276, 1740
BstKTI	GATC	6 cut(s)	253, 355, 470, 1164, 1893, 2522
BstMAI	GTCTC	9 cut(s)	9, 77, 317, 631, 717, 1654, 2023, 2396, 2516
BstMBI	GATC	6 cut(s)	250, 352, 467, 1161, 1890, 2519
BstMCI	CGRYCG	1 cut(s)	1946
BstNI	CCWGG	2 cut(s)	972, 1733
BstPI	GGTNACC	1 cut(s)	1209
BstSCI	CCNGG	3 cut(s)	242, 970, 1731
BstSNI	TACGTA	1 cut(s)	2398
BstV1I	GCAGC	9 cut(s)	151, 191, 250, 350, 410, 1145, 1259, 1519, 2088
BstV2I	GAAGAC	2 cut(s)	546, 2420
BstX2I	RGATCY	2 cut(s)	250, 467
BstYI	RGATCY	2 cut(s)	250, 467
BsuRI	GGCC	5 cut(s)	439, 1022, 1236, 2267, 2546
BtgI	CCRYGG	1 cut(s)	168
BtsCI	GGATG	6 cut(s)	679, 1038, 1183, 1656, 2008, 2552
BtsI	GCAGTG	2 cut(s)	1908, 2342
BtsIMutI	CAGTG	6 cut(s)	756, 897, 1136, 1908, 2091, 2342
BveI	ACCTGC	1 cut(s)	2483
Cac8I	GCNNGC	4 cut(s)	460, 573, 2544, 2573
CfoI	GCGC	2 cut(s)	1276, 1740
Cfr13I	GGNCC	2 cut(s)	2009, 2495
Csp6I	GTAC	3 cut(s)	1053, 1433, 1472
CviAII	CATG	8 cut(s)	169, 319, 356, 1647, 1671, 1927, 2158, 2448
CviQI	GTAC	3 cut(s)	1053, 1433, 1472
DpnI	GATC	6 cut(s)	252, 354, 469, 1163, 1892, 2521
DpnII	GATC	6 cut(s)	250, 352, 467, 1161, 1890, 2519
DraIII	CACNNNGTG	1 cut(s)	2424
EaeI	YGGCCR	1 cut(s)	437
Eam1104I	CTCTTC	6 cut(s)	51, 273, 597, 678, 990, 1178
EarI	CTCTTC	6 cut(s)	51, 273, 597, 678, 990, 1178
Ecl136II	GAGCTC	2 cut(s)	1899, 2167
Eco105I	TACGTA	1 cut(s)	2398
Eco130I	CCWWGG	2 cut(s)	168, 1237
Eco147I	AGGCCT	3 cut(s)	1022, 1236, 2546
Eco24I	GRGCYC	2 cut(s)	1901, 2169
Eco31I	GGTCTC	2 cut(s)	1654, 2396
Eco47I	GGWCC	2 cut(s)	2009, 2495
Eco47III	AGCGCT	1 cut(s)	1739
Eco53kI	GAGCTC	2 cut(s)	1899, 2167
Eco57I	CTGAAG	1 cut(s)	1046
Eco72I	CACGTG	1 cut(s)	1271
Eco88I	CYCGRG	4 cut(s)	74, 714, 1854, 2327
Eco91I	GGTNACC	1 cut(s)	1209
EcoICRI	GAGCTC	2 cut(s)	1899, 2167
EcoNI	CCTNNNNNAGG	1 cut(s)	1027
EcoO65I	GGTNACC	1 cut(s)	1209
EcoRI	GAATTC	3 cut(s)	920, 932, 1580
EcoRII	CCWGG	2 cut(s)	970, 1731
EcoT14I	CCWWGG	2 cut(s)	168, 1237
EcoT22I	ATGCAT	2 cut(s)	488, 2101
EcoT38I	GRGCYC	2 cut(s)	1901, 2169
ErhI	CCWWGG	2 cut(s)	168, 1237
FaeI	CATG	8 cut(s)	172, 322, 359, 1650, 1674, 1930, 2161, 2451
FaqI	GGGAC	1 cut(s)	798
FatI	CATG	8 cut(s)	168, 318, 355, 1646, 1670, 1926, 2157, 2447
FauNDI	CATATG	3 cut(s)	1387, 1399, 2143
FbaI	TGATCA	1 cut(s)	352
FblI	GTMKAC	2 cut(s)	2154, 2356
Fnu4HI	GCNGC	9 cut(s)	165, 180, 264, 339, 399, 1134, 1248, 1533, 2077
FokI	GGATG	6 cut(s)	686, 1045, 1190, 1663, 2015, 2539
FriOI	GRGCYC	2 cut(s)	1901, 2169
Fsp4HI	GCNGC	9 cut(s)	165, 180, 264, 339, 399, 1134, 1248, 1533, 2077
GlaI	GCGC	2 cut(s)	1275, 1739
GluI	GCNGC	9 cut(s)	165, 180, 264, 339, 399, 1134, 1248, 1533, 2077
GsaI	CCCAGC	3 cut(s)	1246, 1288, 1305
GsuI	CTGGAG	1 cut(s)	1754
HaeII	RGCGCY	1 cut(s)	1741
HaeIII	GGCC	5 cut(s)	439, 1022, 1236, 2267, 2546
HapII	CCGG	3 cut(s)	204, 243, 383
HhaI	GCGC	2 cut(s)	1276, 1740
Hin1II	CATG	8 cut(s)	172, 322, 359, 1650, 1674, 1930, 2161, 2451
Hin6I	GCGC	2 cut(s)	1274, 1738
HinP1I	GCGC	2 cut(s)	1274, 1738
HindIII	AAGCTT	3 cut(s)	187, 695, 2365
HpaII	CCGG	3 cut(s)	204, 243, 383
HphI	GGTGA	6 cut(s)	46, 820, 863, 1203, 1553, 1699
Hpy166II	GTNNAC	3 cut(s)	218, 2155, 2357
Hpy8I	GTNNAC	3 cut(s)	218, 2155, 2357
Hpy99I	CGWCG	1 cut(s)	548
HpyCH4III	ACNGT	9 cut(s)	415, 548, 751, 1009, 1140, 1406, 1432, 1484, 2537
HpyCH4IV	ACGT	3 cut(s)	114, 1270, 2397
HpySE526I	ACGT	3 cut(s)	114, 1270, 2397
Hsp92II	CATG	8 cut(s)	172, 322, 359, 1650, 1674, 1930, 2161, 2451
HspAI	GCGC	2 cut(s)	1274, 1738
Ksp22I	TGATCA	1 cut(s)	352
Kzo9I	GATC	6 cut(s)	250, 352, 467, 1161, 1890, 2519
LguI	GCTCTTC	3 cut(s)	597, 678, 990
LmnI	GCTCC	2 cut(s)	119, 1735
Lsp1109I	GCAGC	9 cut(s)	151, 191, 250, 350, 410, 1145, 1259, 1519, 2088
LweI	GCATC	5 cut(s)	589, 1023, 1519, 2041, 2086
MaeII	ACGT	3 cut(s)	114, 1270, 2397
MaeIII	GTNAC	6 cut(s)	106, 751, 851, 1209, 1334, 1957
MalI	GATC	6 cut(s)	252, 354, 469, 1163, 1892, 2521
MboI	GATC	6 cut(s)	250, 352, 467, 1161, 1890, 2519
MflI	RGATCY	2 cut(s)	250, 467
MhlI	GDGCHC	2 cut(s)	1901, 2169
MlsI	TGGCCA	1 cut(s)	439
MluNI	TGGCCA	1 cut(s)	439
MlyI	GAGTC	5 cut(s)	233, 809, 851, 911, 2317
MmeI	TCCRAC	3 cut(s)	97, 1122, 2556
Mox20I	TGGCCA	1 cut(s)	439
Mph1103I	ATGCAT	2 cut(s)	488, 2101
MroXI	GAANNNNTTC	2 cut(s)	932, 1378
MscI	TGGCCA	1 cut(s)	439
MseI	TTAA	8 cut(s)	129, 1103, 1199, 1479, 1703, 1952, 1973, 2376
MslI	CAYNNNNRTG	3 cut(s)	413, 511, 2581
Msp20I	TGGCCA	1 cut(s)	439
MspA1I	CMGCKG	3 cut(s)	182, 1331, 1964
MspI	CCGG	3 cut(s)	204, 243, 383
MspR9I	CCNGG	3 cut(s)	244, 972, 1733
Mva1269I	GAATGC	3 cut(s)	1296, 2099, 2573
MvaI	CCWGG	2 cut(s)	972, 1733
NciI	CCSGG	1 cut(s)	244
NcoI	CCATGG	1 cut(s)	168
NdeI	CATATG	3 cut(s)	1387, 1399, 2143
NdeII	GATC	6 cut(s)	250, 352, 467, 1161, 1890, 2519
NheI	GCTAGC	1 cut(s)	571
NlaIII	CATG	8 cut(s)	172, 322, 359, 1650, 1674, 1930, 2161, 2451
NlaIV	GGNNCC	4 cut(s)	42, 1326, 1696, 1730
NmuCI	GTSAC	4 cut(s)	106, 751, 851, 1209
NsiI	ATGCAT	2 cut(s)	488, 2101
PaeR7I	CTCGAG	1 cut(s)	74
PceI	AGGCCT	3 cut(s)	1022, 1236, 2546
PciSI	GCTCTTC	3 cut(s)	597, 678, 990
PcsI	WCGNNNNNNNCGW	1 cut(s)	1488
PctI	GAATGC	3 cut(s)	1296, 2099, 2573
PdmI	GAANNNNTTC	2 cut(s)	932, 1378
PfeI	GAWTC	8 cut(s)	137, 619, 879, 1145, 1155, 1640, 1774, 2064
PflMI	CCANNNNNTGG	1 cut(s)	1220
PfoI	TCCNGGA	1 cut(s)	1731
PkrI	GCNGC	9 cut(s)	166, 181, 265, 340, 400, 1135, 1249, 1534, 2078
PleI	GAGTC	5 cut(s)	233, 808, 851, 910, 2317
PmaCI	CACGTG	1 cut(s)	1271
PmlI	CACGTG	1 cut(s)	1271
PpsI	GAGTC	5 cut(s)	233, 808, 851, 910, 2317
Ppu21I	YACGTR	2 cut(s)	1271, 2398
PshBI	ATTAAT	1 cut(s)	129
PsiI	TTATAA	1 cut(s)	1515
Psp124BI	GAGCTC	2 cut(s)	1901, 2169
Psp1406I	AACGTT	1 cut(s)	114
Psp6I	CCWGG	2 cut(s)	970, 1731
PspCI	CACGTG	1 cut(s)	1271
PspEI	GGTNACC	1 cut(s)	1209
PspFI	CCCAGC	3 cut(s)	1242, 1284, 1301
PspGI	CCWGG	2 cut(s)	970, 1731
PspN4I	GGNNCC	4 cut(s)	42, 1326, 1696, 1730
PspPI	GGNCC	2 cut(s)	2009, 2495
PsuI	RGATCY	2 cut(s)	250, 467
PvuII	CAGCTG	3 cut(s)	182, 1331, 1964
RsaI	GTAC	3 cut(s)	1054, 1434, 1473
RsaNI	GTAC	3 cut(s)	1053, 1433, 1472
RseI	CAYNNNNRTG	3 cut(s)	413, 511, 2581
SacI	GAGCTC	2 cut(s)	1901, 2169
SapI	GCTCTTC	3 cut(s)	597, 678, 990
SaqAI	TTAA	8 cut(s)	129, 1103, 1199, 1479, 1703, 1952, 1973, 2376
SatI	GCNGC	9 cut(s)	165, 180, 264, 339, 399, 1134, 1248, 1533, 2077
Sau3AI	GATC	6 cut(s)	250, 352, 467, 1161, 1890, 2519
Sau96I	GGNCC	2 cut(s)	2009, 2495
SchI	GAGTC	5 cut(s)	233, 809, 851, 911, 2317
ScrFI	CCNGG	3 cut(s)	244, 972, 1733
SduI	GDGCHC	2 cut(s)	1901, 2169
SfaNI	GCATC	5 cut(s)	589, 1023, 1519, 2041, 2086
Sfr274I	CTCGAG	1 cut(s)	74
SinI	GGWCC	2 cut(s)	2009, 2495
SlaI	CTCGAG	1 cut(s)	74
SmiMI	CAYNNNNRTG	3 cut(s)	413, 511, 2581
SmlI	CTYRAG	8 cut(s)	74, 846, 1278, 1741, 1894, 1900, 2148, 2368
SmoI	CTYRAG	8 cut(s)	74, 846, 1278, 1741, 1894, 1900, 2148, 2368
SnaBI	TACGTA	1 cut(s)	2398
SpeI	ACTAGT	2 cut(s)	1049, 1712
SseBI	AGGCCT	3 cut(s)	1022, 1236, 2546
SsiI	CCGC	2 cut(s)	2406, 2540
SstI	GAGCTC	2 cut(s)	1901, 2169
StuI	AGGCCT	3 cut(s)	1022, 1236, 2546
StyD4I	CCNGG	3 cut(s)	242, 970, 1731
StyI	CCWWGG	2 cut(s)	168, 1237
TaaI	ACNGT	9 cut(s)	415, 548, 751, 1009, 1140, 1406, 1432, 1484, 2537
TaiI	ACGT	3 cut(s)	117, 1273, 2400
TaqI	TCGA	6 cut(s)	75, 653, 988, 2049, 2518, 2565
TaqII	GACCGA	2 cut(s)	902, 1960
TatI	WGTACW	1 cut(s)	1052
TfiI	GAWTC	8 cut(s)	137, 619, 879, 1145, 1155, 1640, 1774, 2064
Tru1I	TTAA	8 cut(s)	129, 1103, 1199, 1479, 1703, 1952, 1973, 2376
Tru9I	TTAA	8 cut(s)	129, 1103, 1199, 1479, 1703, 1952, 1973, 2376
TscAI	CASTG	6 cut(s)	756, 904, 1143, 1915, 2098, 2342
TseFI	GTSAC	4 cut(s)	106, 751, 851, 1209
TseI	GCWGC	9 cut(s)	164, 179, 263, 338, 398, 1133, 1247, 1532, 2076
Tsp45I	GTSAC	4 cut(s)	106, 751, 851, 1209
TspGWI	ACGGA	1 cut(s)	2545
TspRI	CASTG	6 cut(s)	756, 904, 1143, 1915, 2098, 2342
Van91I	CCANNNNNTGG	1 cut(s)	1220
VpaK11BI	GGWCC	2 cut(s)	2009, 2495
VspI	ATTAAT	1 cut(s)	129
XagI	CCTNNNNNAGG	1 cut(s)	1027
XbaI	TCTAGA	1 cut(s)	2272
XhoI	CTCGAG	1 cut(s)	74
XmaJI	CCTAGG	1 cut(s)	1237
XmiI	GTMKAC	2 cut(s)	2154, 2356
XmnI	GAANNNNTTC	2 cut(s)	932, 1378
Zsp2I	ATGCAT	2 cut(s)	488, 2101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.