Glutamate-gated receptor that probably acts as non- selective cation channel
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 2021 |
| Acc36I | ACCTGC | 1 cut(s) | 2043 |
| AccB1I | GGYRCC | 1 cut(s) | 1342 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1772 |
| AccBSI | CCGCTC | 1 cut(s) | 2517 |
| AccI | GTMKAC | 1 cut(s) | 543 |
| AccII | CGCG | 4 cut(s) | 23, 249, 1870, 2513 |
| AccIII | TCCGGA | 1 cut(s) | 927 |
| AciI | CCGC | 9 cut(s) | 180, 500, 564, 644, 1111, 1122, 1926, 2124, 2517 |
| AclI | AACGTT | 1 cut(s) | 147 |
| AclWI | GGATC | 1 cut(s) | 1900 |
| AcoI | YGGCCR | 2 cut(s) | 446, 2550 |
| AcsI | RAATTY | 9 cut(s) | 387, 922, 935, 978, 1367, 1595, 1769, 2099, 2555 |
| AcuI | CTGAAG | 2 cut(s) | 309, 1345 |
| AcvI | CACGTG | 1 cut(s) | 2252 |
| AfaI | GTAC | 5 cut(s) | 1131, 1136, 1505, 1902, 2033 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1211, 1534, 1772 |
| AgeI | ACCGGT | 1 cut(s) | 2772 |
| AhlI | ACTAGT | 2 cut(s) | 908, 1286 |
| AjnI | CCWGG | 5 cut(s) | 56, 973, 1388, 1746, 2651 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 309, 341 |
| AleI | CACNNNNGTG | 1 cut(s) | 2264 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1199, 1231 |
| Alw21I | GWGCWC | 3 cut(s) | 630, 2596, 2646 |
| Alw26I | GTCTC | 3 cut(s) | 17, 255, 329 |
| AlwI | GGATC | 1 cut(s) | 1900 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1960 |
| Ama87I | CYCGRG | 3 cut(s) | 717, 1274, 2681 |
| Aor13HI | TCCGGA | 1 cut(s) | 927 |
| AoxI | GGCC | 8 cut(s) | 446, 597, 1023, 1271, 1777, 2121, 2395, 2550 |
| ApeKI | GCWGC | 8 cut(s) | 194, 275, 284, 350, 407, 2278, 2414, 2483 |
| ApoI | RAATTY | 9 cut(s) | 387, 922, 935, 978, 1367, 1595, 1769, 2099, 2555 |
| AseI | ATTAAT | 1 cut(s) | 2577 |
| AsiGI | ACCGGT | 1 cut(s) | 2772 |
| Asp700I | GAANNNNTTC | 5 cut(s) | 935, 1015, 1178, 1679, 2099 |
| AspLEI | GCGC | 5 cut(s) | 23, 137, 968, 1755, 2513 |
| AspS9I | GGNCC | 5 cut(s) | 598, 1272, 1386, 1778, 2655 |
| AsuC2I | CCSGG | 3 cut(s) | 1275, 1276, 1347 |
| AsuII | TTCGAA | 1 cut(s) | 1485 |
| AsuNHI | GCTAGC | 2 cut(s) | 580, 1570 |
| AvaI | CYCGRG | 3 cut(s) | 717, 1274, 2681 |
| AvaII | GGWCC | 2 cut(s) | 1386, 2655 |
| BaeGI | GKGCMC | 2 cut(s) | 875, 1294 |
| BalI | TGGCCA | 2 cut(s) | 448, 2552 |
| BanI | GGYRCC | 1 cut(s) | 1342 |
| BanII | GRGCYC | 1 cut(s) | 2596 |
| BbrPI | CACGTG | 1 cut(s) | 2252 |
| BbsI | GAAGAC | 1 cut(s) | 2146 |
| Bbv12I | GWGCWC | 3 cut(s) | 630, 2596, 2646 |
| BbvCI | CCTCAGC | 1 cut(s) | 2459 |
| BbvI | GCAGC | 8 cut(s) | 206, 262, 271, 362, 419, 2265, 2401, 2470 |
| BccI | CCATC | 6 cut(s) | 438, 457, 548, 778, 1490, 1721 |
| BceAI | ACGGC | 3 cut(s) | 1135, 1459, 2101 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 812, 846 |
| BciT130I | CCWGG | 5 cut(s) | 58, 975, 1390, 1748, 2653 |
| BclI | TGATCA | 1 cut(s) | 297 |
| BcnI | CCSGG | 3 cut(s) | 1275, 1276, 1347 |
| BcoDI | GTCTC | 3 cut(s) | 17, 255, 329 |
| BcuI | ACTAGT | 2 cut(s) | 908, 1286 |
| BfaI | CTAG | 7 cut(s) | 581, 683, 909, 1053, 1287, 1571, 2273 |
| BfmI | CTRYAG | 5 cut(s) | 1855, 2036, 2115, 2200, 2484 |
| BfoI | RGCGCY | 2 cut(s) | 969, 1756 |
| BfuAI | ACCTGC | 1 cut(s) | 2043 |
| Bme1390I | CCNGG | 8 cut(s) | 58, 975, 1275, 1276, 1347, 1390, 1748, 2653 |
| Bme18I | GGWCC | 2 cut(s) | 1386, 2655 |
| BmeT110I | CYCGRG | 3 cut(s) | 717, 1274, 2681 |
| BmgT120I | GGNCC | 5 cut(s) | 598, 1272, 1386, 1778, 2655 |
| BmiI | GGNNCC | 5 cut(s) | 1280, 1344, 1711, 1745, 1780 |
| BmrFI | CCNGG | 8 cut(s) | 58, 975, 1275, 1276, 1347, 1390, 1748, 2653 |
| BmrI | ACTGGG | 1 cut(s) | 682 |
| BmsI | GCATC | 4 cut(s) | 567, 991, 1534, 2695 |
| BmtI | GCTAGC | 2 cut(s) | 584, 1574 |
| BmuI | ACTGGG | 1 cut(s) | 682 |
| BpiI | GAAGAC | 1 cut(s) | 2146 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2224, 2256 |
| BpmI | CTGGAG | 4 cut(s) | 719, 1125, 1769, 2303 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2459 |
| Bpu14I | TTCGAA | 1 cut(s) | 1485 |
| BpuEI | CTTGAG | 4 cut(s) | 339, 1079, 1900, 2236 |
| BpuMI | CCSGG | 3 cut(s) | 1275, 1276, 1347 |
| BsaAI | YACGTR | 1 cut(s) | 2252 |
| BsaBI | GATNNNNATC | 1 cut(s) | 448 |
| BsaI | GGTCTC | 2 cut(s) | 17, 255 |
| BsaJI | CCNNGG | 6 cut(s) | 56, 183, 594, 1274, 2047, 2633 |
| BsaWI | WCCGGW | 4 cut(s) | 391, 927, 1204, 2772 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1742, 1772, 1976, 2006, 2578, 2608 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1211, 1534, 1772 |
| Bse118I | RCCGGY | 2 cut(s) | 1578, 2772 |
| Bse1I | ACTGG | 4 cut(s) | 677, 736, 1142, 1711 |
| Bse3DI | GCAATG | 3 cut(s) | 495, 1314, 1428 |
| Bse8I | GATNNNNATC | 1 cut(s) | 448 |
| BseAI | TCCGGA | 1 cut(s) | 927 |
| BseBI | CCWGG | 5 cut(s) | 58, 975, 1390, 1748, 2653 |
| BseDI | CCNNGG | 6 cut(s) | 56, 183, 594, 1274, 2047, 2633 |
| BseGI | GGATG | 5 cut(s) | 582, 1174, 1231, 1671, 2686 |
| BseJI | GATNNNNATC | 1 cut(s) | 448 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1211, 1534, 1772 |
| BseMI | GCAATG | 3 cut(s) | 495, 1314, 1428 |
| BseMII | CTCAG | 4 cut(s) | 189, 1413, 1616, 2450 |
| BseNI | ACTGG | 4 cut(s) | 677, 736, 1142, 1711 |
| BseRI | GAGGAG | 2 cut(s) | 1813, 2476 |
| BseSI | GKGCMC | 2 cut(s) | 875, 1294 |
| BseXI | GCAGC | 8 cut(s) | 206, 262, 271, 362, 419, 2265, 2401, 2470 |
| BseYI | CCCAGC | 2 cut(s) | 969, 1302 |
| BsgI | GTGCAG | 1 cut(s) | 2175 |
| Bsh1236I | CGCG | 4 cut(s) | 23, 249, 1870, 2513 |
| BshFI | GGCC | 8 cut(s) | 448, 599, 1025, 1273, 1779, 2123, 2397, 2552 |
| BshNI | GGYRCC | 1 cut(s) | 1342 |
| BshTI | ACCGGT | 1 cut(s) | 2772 |
| BsiHKAI | GWGCWC | 3 cut(s) | 630, 2596, 2646 |
| BsiHKCI | CYCGRG | 3 cut(s) | 717, 1274, 2681 |
| BsiSI | CCGG | 9 cut(s) | 255, 392, 928, 1205, 1275, 1346, 1579, 1640, 2773 |
| BslFI | GGGAC | 2 cut(s) | 801, 2027 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1211, 1534, 1772 |
| BsmAI | GTCTC | 3 cut(s) | 17, 255, 329 |
| BsmFI | GGGAC | 2 cut(s) | 801, 2027 |
| BsmI | GAATGC | 2 cut(s) | 1975, 2726 |
| BsnI | GGCC | 8 cut(s) | 448, 599, 1025, 1273, 1779, 2123, 2397, 2552 |
| Bso31I | GGTCTC | 2 cut(s) | 17, 255 |
| BsoBI | CYCGRG | 3 cut(s) | 717, 1274, 2681 |
| Bsp119I | TTCGAA | 1 cut(s) | 1485 |
| Bsp1286I | GDGCHC | 5 cut(s) | 630, 875, 1294, 2596, 2646 |
| Bsp13I | TCCGGA | 1 cut(s) | 927 |
| Bsp143I | GATC | 7 cut(s) | 189, 297, 364, 813, 1381, 1510, 1905 |
| Bsp19I | CCATGG | 1 cut(s) | 183 |
| Bsp68I | TCGCGA | 1 cut(s) | 249 |
| BspACI | CCGC | 9 cut(s) | 180, 500, 564, 644, 1111, 1122, 1926, 2124, 2517 |
| BspANI | GGCC | 8 cut(s) | 448, 599, 1025, 1273, 1779, 2123, 2397, 2552 |
| BspCNI | CTCAG | 4 cut(s) | 190, 1412, 1615, 2451 |
| BspEI | TCCGGA | 1 cut(s) | 927 |
| BspFNI | CGCG | 4 cut(s) | 23, 249, 1870, 2513 |
| BspHI | TCATGA | 1 cut(s) | 2157 |
| BspLI | GGNNCC | 5 cut(s) | 1280, 1344, 1711, 1745, 1780 |
| BspMAI | CTGCAG | 2 cut(s) | 2040, 2488 |
| BspMI | ACCTGC | 1 cut(s) | 2043 |
| BspOI | GCTAGC | 2 cut(s) | 584, 1574 |
| BspPI | GGATC | 1 cut(s) | 1900 |
| BspQI | GCTCTTC | 1 cut(s) | 993 |
| BspT104I | TTCGAA | 1 cut(s) | 1485 |
| BspT107I | GGYRCC | 1 cut(s) | 1342 |
| BspTNI | GGTCTC | 2 cut(s) | 17, 255 |
| BsrBI | CCGCTC | 1 cut(s) | 2517 |
| BsrDI | GCAATG | 3 cut(s) | 495, 1314, 1428 |
| BsrFI | RCCGGY | 2 cut(s) | 1578, 2772 |
| BsrI | ACTGG | 4 cut(s) | 677, 736, 1142, 1711 |
| BssAI | RCCGGY | 2 cut(s) | 1578, 2772 |
| BssECI | CCNNGG | 6 cut(s) | 56, 183, 594, 1274, 2047, 2633 |
| BssMI | GATC | 7 cut(s) | 189, 297, 364, 813, 1381, 1510, 1905 |
| BssNAI | GTATAC | 1 cut(s) | 544 |
| BssT1I | CCWWGG | 3 cut(s) | 183, 2047, 2633 |
| Bst1107I | GTATAC | 1 cut(s) | 544 |
| Bst2UI | CCWGG | 5 cut(s) | 58, 975, 1390, 1748, 2653 |
| Bst4CI | ACNGT | 9 cut(s) | 632, 754, 1075, 1134, 1318, 1567, 1729, 1951, 2387 |
| Bst6I | CTCTTC | 3 cut(s) | 285, 681, 993 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1001 |
| BstBAI | YACGTR | 1 cut(s) | 2252 |
| BstBI | TTCGAA | 1 cut(s) | 1485 |
| BstC8I | GCNNGC | 5 cut(s) | 106, 582, 1572, 2515, 2542 |
| BstDEI | CTNAG | 4 cut(s) | 198, 1399, 1602, 2459 |
| BstDSI | CCRYGG | 1 cut(s) | 183 |
| BstF5I | GGATG | 5 cut(s) | 582, 1174, 1231, 1671, 2686 |
| BstFNI | CGCG | 4 cut(s) | 23, 249, 1870, 2513 |
| BstH2I | RGCGCY | 2 cut(s) | 969, 1756 |
| BstHHI | GCGC | 5 cut(s) | 23, 137, 968, 1755, 2513 |
| BstKTI | GATC | 7 cut(s) | 192, 300, 367, 816, 1384, 1513, 1908 |
| BstMAI | GTCTC | 3 cut(s) | 17, 255, 329 |
| BstMBI | GATC | 7 cut(s) | 189, 297, 364, 813, 1381, 1510, 1905 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 255, 281, 284, 359, 564, 992, 1001, 2085, 2612 |
| BstNI | CCWGG | 5 cut(s) | 58, 975, 1390, 1748, 2653 |
| BstNSI | RCATGY | 1 cut(s) | 1111 |
| BstSCI | CCNGG | 8 cut(s) | 56, 973, 1273, 1274, 1345, 1388, 1746, 2651 |
| BstSFI | CTRYAG | 5 cut(s) | 1855, 2036, 2115, 2200, 2484 |
| BstSLI | GKGCMC | 2 cut(s) | 875, 1294 |
| BstUI | CGCG | 4 cut(s) | 23, 249, 1870, 2513 |
| BstV1I | GCAGC | 8 cut(s) | 206, 262, 271, 362, 419, 2265, 2401, 2470 |
| BstV2I | GAAGAC | 1 cut(s) | 2146 |
| BstZ17I | GTATAC | 1 cut(s) | 544 |
| BsuRI | GGCC | 8 cut(s) | 448, 599, 1025, 1273, 1779, 2123, 2397, 2552 |
| BtgI | CCRYGG | 1 cut(s) | 183 |
| BtgZI | GCGATG | 1 cut(s) | 582 |
| BtsCI | GGATG | 5 cut(s) | 582, 1174, 1231, 1671, 2686 |
| BtsI | GCAGTG | 2 cut(s) | 2187, 2503 |
| BtsIMutI | CAGTG | 4 cut(s) | 1255, 1734, 2187, 2503 |
| BtuMI | TCGCGA | 1 cut(s) | 249 |
| BveI | ACCTGC | 1 cut(s) | 2043 |
| Cac8I | GCNNGC | 5 cut(s) | 106, 582, 1572, 2515, 2542 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1960 |
| CciI | TCATGA | 1 cut(s) | 2157 |
| CfoI | GCGC | 5 cut(s) | 23, 137, 968, 1755, 2513 |
| Cfr10I | RCCGGY | 2 cut(s) | 1578, 2772 |
| Cfr13I | GGNCC | 5 cut(s) | 598, 1272, 1386, 1778, 2655 |
| Cfr9I | CCCGGG | 1 cut(s) | 1274 |
| Csp6I | GTAC | 5 cut(s) | 1130, 1135, 1504, 1901, 2032 |
| CspAI | ACCGGT | 1 cut(s) | 2772 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 112, 147 |
| CviQI | GTAC | 5 cut(s) | 1130, 1135, 1504, 1901, 2032 |
| DdeI | CTNAG | 4 cut(s) | 198, 1399, 1602, 2459 |
| DpnI | GATC | 7 cut(s) | 191, 299, 366, 815, 1383, 1512, 1907 |
| DpnII | GATC | 7 cut(s) | 189, 297, 364, 813, 1381, 1510, 1905 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 2021 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 2021 |
| EaeI | YGGCCR | 2 cut(s) | 446, 2550 |
| Eam1104I | CTCTTC | 3 cut(s) | 285, 681, 993 |
| EarI | CTCTTC | 3 cut(s) | 285, 681, 993 |
| Ecl136II | GAGCTC | 1 cut(s) | 2594 |
| Eco130I | CCWWGG | 3 cut(s) | 183, 2047, 2633 |
| Eco147I | AGGCCT | 1 cut(s) | 2397 |
| Eco24I | GRGCYC | 1 cut(s) | 2596 |
| Eco31I | GGTCTC | 2 cut(s) | 17, 255 |
| Eco47I | GGWCC | 2 cut(s) | 1386, 2655 |
| Eco53kI | GAGCTC | 1 cut(s) | 2594 |
| Eco57I | CTGAAG | 2 cut(s) | 309, 1345 |
| Eco72I | CACGTG | 1 cut(s) | 2252 |
| Eco88I | CYCGRG | 3 cut(s) | 717, 1274, 2681 |
| EcoICRI | GAGCTC | 1 cut(s) | 2594 |
| EcoO109I | RGGNCCY | 1 cut(s) | 598 |
| EcoRI | GAATTC | 2 cut(s) | 1595, 2099 |
| EcoRII | CCWGG | 5 cut(s) | 56, 973, 1388, 1746, 2651 |
| EcoT14I | CCWWGG | 3 cut(s) | 183, 2047, 2633 |
| EcoT22I | ATGCAT | 1 cut(s) | 497 |
| EcoT38I | GRGCYC | 1 cut(s) | 2596 |
| ErhI | CCWWGG | 3 cut(s) | 183, 2047, 2633 |
| FaqI | GGGAC | 2 cut(s) | 801, 2027 |
| FauNDI | CATATG | 3 cut(s) | 472, 997, 2143 |
| FbaI | TGATCA | 1 cut(s) | 297 |
| FblI | GTMKAC | 1 cut(s) | 543 |
| FokI | GGATG | 5 cut(s) | 589, 1181, 1238, 1678, 2673 |
| FriOI | GRGCYC | 1 cut(s) | 2596 |
| FspBI | CTAG | 7 cut(s) | 581, 683, 909, 1053, 1287, 1571, 2273 |
| GlaI | GCGC | 5 cut(s) | 22, 136, 967, 1754, 2512 |
| GsaI | CCCAGC | 2 cut(s) | 973, 1306 |
| GsuI | CTGGAG | 4 cut(s) | 719, 1125, 1769, 2303 |
| HaeII | RGCGCY | 2 cut(s) | 969, 1756 |
| HaeIII | GGCC | 8 cut(s) | 448, 599, 1025, 1273, 1779, 2123, 2397, 2552 |
| HapII | CCGG | 9 cut(s) | 255, 392, 928, 1205, 1275, 1346, 1579, 1640, 2773 |
| HhaI | GCGC | 5 cut(s) | 23, 137, 968, 1755, 2513 |
| Hin6I | GCGC | 5 cut(s) | 21, 135, 966, 1753, 2511 |
| HinP1I | GCGC | 5 cut(s) | 21, 135, 966, 1753, 2511 |
| HincII | GTYRAC | 2 cut(s) | 151, 2322 |
| HindII | GTYRAC | 2 cut(s) | 151, 2322 |
| HindIII | AAGCTT | 2 cut(s) | 230, 2371 |
| HpaI | GTTAAC | 1 cut(s) | 151 |
| HpaII | CCGG | 9 cut(s) | 255, 392, 928, 1205, 1275, 1346, 1579, 1640, 2773 |
| Hpy166II | GTNNAC | 5 cut(s) | 151, 544, 1815, 2322, 2342 |
| Hpy188I | TCNGA | 9 cut(s) | 374, 486, 862, 955, 1225, 1402, 1794, 2508, 2697 |
| Hpy8I | GTNNAC | 5 cut(s) | 151, 544, 1815, 2322, 2342 |
| Hpy99I | CGWCG | 1 cut(s) | 2012 |
| HpyAV | CCTTC | 9 cut(s) | 248, 611, 862, 950, 1369, 1369, 1828, 2168, 2326 |
| HpyCH4III | ACNGT | 9 cut(s) | 632, 754, 1075, 1134, 1318, 1567, 1729, 1951, 2387 |
| HpyCH4IV | ACGT | 2 cut(s) | 147, 2251 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 255, 281, 284, 359, 564, 992, 1001, 2085, 2612 |
| HpyF3I | CTNAG | 4 cut(s) | 198, 1399, 1602, 2459 |
| HpySE526I | ACGT | 2 cut(s) | 147, 2251 |
| HspAI | GCGC | 5 cut(s) | 21, 135, 966, 1753, 2511 |
| Kpn2I | TCCGGA | 1 cut(s) | 927 |
| Ksp22I | TGATCA | 1 cut(s) | 297 |
| KspAI | GTTAAC | 1 cut(s) | 151 |
| Kzo9I | GATC | 7 cut(s) | 189, 297, 364, 813, 1381, 1510, 1905 |
| LguI | GCTCTTC | 1 cut(s) | 993 |
| LmnI | GCTCC | 3 cut(s) | 1750, 2599, 2641 |
| Lsp1109I | GCAGC | 8 cut(s) | 206, 262, 271, 362, 419, 2265, 2401, 2470 |
| LweI | GCATC | 4 cut(s) | 567, 991, 1534, 2695 |
| MaeI | CTAG | 7 cut(s) | 581, 683, 909, 1053, 1287, 1571, 2273 |
| MaeII | ACGT | 2 cut(s) | 147, 2251 |
| MaeIII | GTNAC | 4 cut(s) | 754, 1556, 1702, 2669 |
| MalI | GATC | 7 cut(s) | 191, 299, 366, 815, 1383, 1512, 1907 |
| MbiI | CCGCTC | 1 cut(s) | 2517 |
| MboI | GATC | 7 cut(s) | 189, 297, 364, 813, 1381, 1510, 1905 |
| MhlI | GDGCHC | 5 cut(s) | 630, 875, 1294, 2596, 2646 |
| MlsI | TGGCCA | 2 cut(s) | 448, 2552 |
| MluNI | TGGCCA | 2 cut(s) | 448, 2552 |
| MlyI | GAGTC | 9 cut(s) | 217, 245, 812, 1391, 1625, 1985, 2009, 2317, 2783 |
| MmeI | TCCRAC | 5 cut(s) | 352, 775, 2609, 2621, 2768 |
| Mox20I | TGGCCA | 2 cut(s) | 448, 2552 |
| Mph1103I | ATGCAT | 1 cut(s) | 497 |
| MroI | TCCGGA | 1 cut(s) | 927 |
| MroXI | GAANNNNTTC | 5 cut(s) | 935, 1015, 1178, 1679, 2099 |
| MscI | TGGCCA | 2 cut(s) | 448, 2552 |
| MseI | TTAA | 6 cut(s) | 144, 150, 1115, 1718, 1865, 2577 |
| MslI | CAYNNNNRTG | 4 cut(s) | 1565, 1940, 2069, 2264 |
| Msp20I | TGGCCA | 2 cut(s) | 448, 2552 |
| MspA1I | CMGCKG | 2 cut(s) | 197, 2414 |
| MspI | CCGG | 9 cut(s) | 255, 392, 928, 1205, 1275, 1346, 1579, 1640, 2773 |
| MspR9I | CCNGG | 8 cut(s) | 58, 975, 1275, 1276, 1347, 1390, 1748, 2653 |
| Mva1269I | GAATGC | 2 cut(s) | 1975, 2726 |
| MvaI | CCWGG | 5 cut(s) | 58, 975, 1390, 1748, 2653 |
| MvnI | CGCG | 4 cut(s) | 23, 249, 1870, 2513 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 255, 281, 284, 359, 564, 992, 1001, 2085, 2612 |
| NciI | CCSGG | 3 cut(s) | 1275, 1276, 1347 |
| NcoI | CCATGG | 1 cut(s) | 183 |
| NdeI | CATATG | 3 cut(s) | 472, 997, 2143 |
| NdeII | GATC | 7 cut(s) | 189, 297, 364, 813, 1381, 1510, 1905 |
| NheI | GCTAGC | 2 cut(s) | 580, 1570 |
| NlaIV | GGNNCC | 5 cut(s) | 1280, 1344, 1711, 1745, 1780 |
| NmuCI | GTSAC | 3 cut(s) | 754, 1556, 1702 |
| NruI | TCGCGA | 1 cut(s) | 249 |
| NsiI | ATGCAT | 1 cut(s) | 497 |
| NspI | RCATGY | 1 cut(s) | 1111 |
| NspV | TTCGAA | 1 cut(s) | 1485 |
| OliI | CACNNNNGTG | 1 cut(s) | 2264 |
| PagI | TCATGA | 1 cut(s) | 2157 |
| PceI | AGGCCT | 1 cut(s) | 2397 |
| PciSI | GCTCTTC | 1 cut(s) | 993 |
| PctI | GAATGC | 2 cut(s) | 1975, 2726 |
| PdmI | GAANNNNTTC | 5 cut(s) | 935, 1015, 1178, 1679, 2099 |
| PfeI | GAWTC | 9 cut(s) | 25, 30, 882, 1011, 1179, 1245, 1789, 2052, 2269 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1772 |
| PfoI | TCCNGGA | 1 cut(s) | 1746 |
| PinAI | ACCGGT | 1 cut(s) | 2772 |
| PleI | GAGTC | 9 cut(s) | 217, 245, 811, 1391, 1624, 1985, 2009, 2317, 2783 |
| PmaCI | CACGTG | 1 cut(s) | 2252 |
| PmlI | CACGTG | 1 cut(s) | 2252 |
| PpsI | GAGTC | 9 cut(s) | 217, 245, 811, 1391, 1624, 1985, 2009, 2317, 2783 |
| Ppu21I | YACGTR | 1 cut(s) | 2252 |
| PshBI | ATTAAT | 1 cut(s) | 2577 |
| Psp124BI | GAGCTC | 1 cut(s) | 2596 |
| Psp1406I | AACGTT | 1 cut(s) | 147 |
| Psp6I | CCWGG | 5 cut(s) | 56, 973, 1388, 1746, 2651 |
| PspCI | CACGTG | 1 cut(s) | 2252 |
| PspFI | CCCAGC | 2 cut(s) | 969, 1302 |
| PspGI | CCWGG | 5 cut(s) | 56, 973, 1388, 1746, 2651 |
| PspN4I | GGNNCC | 5 cut(s) | 1280, 1344, 1711, 1745, 1780 |
| PspPI | GGNCC | 5 cut(s) | 598, 1272, 1386, 1778, 2655 |
| PstI | CTGCAG | 2 cut(s) | 2040, 2488 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1960 |
| PvuII | CAGCTG | 2 cut(s) | 197, 2414 |
| RruI | TCGCGA | 1 cut(s) | 249 |
| RsaI | GTAC | 5 cut(s) | 1131, 1136, 1505, 1902, 2033 |
| RsaNI | GTAC | 5 cut(s) | 1130, 1135, 1504, 1901, 2032 |
| RseI | CAYNNNNRTG | 4 cut(s) | 1565, 1940, 2069, 2264 |
| SacI | GAGCTC | 1 cut(s) | 2596 |
| SapI | GCTCTTC | 1 cut(s) | 993 |
| SaqAI | TTAA | 6 cut(s) | 144, 150, 1115, 1718, 1865, 2577 |
| Sau3AI | GATC | 7 cut(s) | 189, 297, 364, 813, 1381, 1510, 1905 |
| Sau96I | GGNCC | 5 cut(s) | 598, 1272, 1386, 1778, 2655 |
| SchI | GAGTC | 9 cut(s) | 217, 245, 812, 1391, 1625, 1985, 2009, 2317, 2783 |
| ScrFI | CCNGG | 8 cut(s) | 58, 975, 1275, 1276, 1347, 1390, 1748, 2653 |
| SduI | GDGCHC | 5 cut(s) | 630, 875, 1294, 2596, 2646 |
| SfaNI | GCATC | 4 cut(s) | 567, 991, 1534, 2695 |
| SfcI | CTRYAG | 5 cut(s) | 1855, 2036, 2115, 2200, 2484 |
| SfuI | TTCGAA | 1 cut(s) | 1485 |
| SinI | GGWCC | 2 cut(s) | 1386, 2655 |
| SmaI | CCCGGG | 1 cut(s) | 1276 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 1565, 1940, 2069, 2264 |
| SmlI | CTYRAG | 4 cut(s) | 354, 1058, 1915, 2215 |
| SmoI | CTYRAG | 4 cut(s) | 354, 1058, 1915, 2215 |
| SpeI | ACTAGT | 2 cut(s) | 908, 1286 |
| SseBI | AGGCCT | 1 cut(s) | 2397 |
| SsiI | CCGC | 9 cut(s) | 180, 500, 564, 644, 1111, 1122, 1926, 2124, 2517 |
| SspI | AATATT | 2 cut(s) | 1162, 2581 |
| SspMI | CTAG | 7 cut(s) | 581, 683, 909, 1053, 1287, 1571, 2273 |
| SstI | GAGCTC | 1 cut(s) | 2596 |
| StuI | AGGCCT | 1 cut(s) | 2397 |
| StyD4I | CCNGG | 8 cut(s) | 56, 973, 1273, 1274, 1345, 1388, 1746, 2651 |
| StyI | CCWWGG | 3 cut(s) | 183, 2047, 2633 |
| TaaI | ACNGT | 9 cut(s) | 632, 754, 1075, 1134, 1318, 1567, 1729, 1951, 2387 |
| TaiI | ACGT | 2 cut(s) | 150, 2254 |
| TaqI | TCGA | 6 cut(s) | 28, 769, 812, 991, 1485, 1983 |
| TaqII | GACCGA | 2 cut(s) | 608, 1374 |
| TatI | WGTACW | 2 cut(s) | 1134, 1900 |
| TauI | GCSGC | 2 cut(s) | 567, 2126 |
| TfiI | GAWTC | 9 cut(s) | 25, 30, 882, 1011, 1179, 1245, 1789, 2052, 2269 |
| Tru1I | TTAA | 6 cut(s) | 144, 150, 1115, 1718, 1865, 2577 |
| Tru9I | TTAA | 6 cut(s) | 144, 150, 1115, 1718, 1865, 2577 |
| TscAI | CASTG | 4 cut(s) | 1255, 1734, 2194, 2503 |
| TseFI | GTSAC | 3 cut(s) | 754, 1556, 1702 |
| TseI | GCWGC | 8 cut(s) | 194, 275, 284, 350, 407, 2278, 2414, 2483 |
| Tsp45I | GTSAC | 3 cut(s) | 754, 1556, 1702 |
| TspMI | CCCGGG | 1 cut(s) | 1274 |
| TspRI | CASTG | 4 cut(s) | 1255, 1734, 2194, 2503 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1772 |
| VpaK11BI | GGWCC | 2 cut(s) | 1386, 2655 |
| VspI | ATTAAT | 1 cut(s) | 2577 |
| XapI | RAATTY | 9 cut(s) | 387, 922, 935, 978, 1367, 1595, 1769, 2099, 2555 |
| XbaI | TCTAGA | 1 cut(s) | 2272 |
| XceI | RCATGY | 1 cut(s) | 1111 |
| XmaI | CCCGGG | 1 cut(s) | 1274 |
| XmiI | GTMKAC | 1 cut(s) | 543 |
| XmnI | GAANNNNTTC | 5 cut(s) | 935, 1015, 1178, 1679, 2099 |
| XspI | CTAG | 7 cut(s) | 581, 683, 909, 1053, 1287, 1571, 2273 |
| Zsp2I | ATGCAT | 1 cut(s) | 497 |