Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000350

Physical Location & Seq

Genomic Coordinates Reverse (-)

43849463 .. 43852695

Gene Structure 3233 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1306400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2547 bp

ATGTCTTTTCCTTTCCCTAAACTTGGGACCTTCGCTCCTGTGCTCATCGTCTACTTGCTAATTGTTATCTCCCATGGAGTTGGAGCTAACAATAGTAGCAATGTCACCAACATTGGAGCAATCATTAATGCTAATGGCCGTATCGGGAAAGAACAGAAAACTGCCATGGAAATTGCAGCTGAAAACTTCAATAACCATTCGAAGACTCATAAGCTAATCCTTCATTTTCGAGACTCTGGCAGAGACCCCTTTCGTGCTGCTTATGCCGCTAAAGAATTGATAAAGAAGAAGGTAGACGTGATAGTTGGCATGGAGACATGGCAGGAAGCAGCTCAAGTAGCTGATCATGGAAACCAATCGAATGTTACGGTGATTTCATTCGCATCGCCAACTATCACCCCGCCCCTAATCCAGCGCCGCTGGCCATATCTAATACGAATGGCAACAGACGGTTCTGCTCAATTAAAAGGCATAGCGGATATAGTTTCTGCGTACCATTGGAAAAGGGTGGTTGTAGTATACGAAGATGATGGTTACGGTGGAGCTGTTGGGATGCTAGCTGTTTTATCTGAGGCTCTTCGAGATGTTGGTTCGGTGATTGAGCACCGTTTGATTCTCCCGCGAGTTTCTTCGATACATAATCCAAACGGGGATGGGTTTGAAGAGATGCTGAACCTTACCACCATAAAATCCCGTGTGTTTATTGTTATTCAGTCATCTTTGCCTACAGTGACTCGTCTGTTTCAGGTAGCGAAGGAGACGGGACTTGTAGGAAGAGACTCAGCTTGGATAATCACAGAGGCTGTCATTAGTAATTTGCCTGACTCTCTTGACAACTCTGATATGGAAGGCACTCTCGGAATGAAGACTGACTATGCTAAAAATACTAGTTCTTATATGAAGTTCCTAAAGGAGTTCGAAAAAAAGTTTTCAGATGAAGATAACATTGTGCCAACGCCAGGAATTTATGCACTTCGAGCCTATGATATGATGACAATCATTACACAAGCCATAGAAAGAATGACTAGTAACAACAGTTCTACTACTACTAGTAGTAGCCTTCAGGTATTGTTAAACACATTGTTGAACAATTATACCGGTCTAAGTGGGGAAATGTACTGCAAAGGAGGTGAGGTACTTTTTCGTTCTCCGACATTCAGGATCATAAATGTTGTTGATGGGAAGAGACAAAGAGATGTAGGGTCTTTAGAAAGCATTGTCAGCCAAGGCACAGACGGAAGTAATGATGTTGGTATAATTTGGCCCGGGAAAGACATTAGTGCCCCGAAAGGTTGGGCTATGCCTACTAAAGAGAGCCCAATGAAAATTTTGGTTCCTGGTATAACACCCTTCAACGCATTTGTAAAGGTTGATTGGTCGCGGAACAATTCAGACGAGAAGAAATTCGATGGTTTCTGCATTGAACTTTTCAGATGGATACTACCAAATTTGACTTATACTCTGCATCATGAAATTGAAGCCTTAAATTGTACTTACGATTCTCTGATAGAACTGGTCCAAAACAAGAGAGAACGTATGCTTTTCATGTTCATCAAAACATTGGTAAAATGGAAGACATATGATGCTGTGATCGGTGATATGACTGTGCTAGCTGATCGGTTGGACAAGGTGGACTTCACTCAGCCATATATCGAGTCTGGTTTGTCGATGATTGTTCCTGAAAAAGATGAGGAGTCGACATGGCTATTTTTGAAGCCTTTCACTTGGCAAATGTGGGCGGTGTCTGGTGGCATTTTACTCTACACAACGCTAATAGTCTGGCTCCTCGAAGGCCCGTCAAATCCAGAATTTGGTGATGGGTTGAAGAATCAAATCAGGACAGCAACTTGGTTCACTTTCTCCTCTTTGTTCTTTGCTCAGAGAGAGAAAATCTCTAGCAACTTTACTAAAGTAGTTGTTATAGAGTGGCTGTTTGTTGTATTGATCCTAAGCTCAAGCTACACTGCTAATCTTTCCTCAATGCTTACCATCAAACGGCTGCAACCAAACGTAACGGACATTGAAATGCTGAAGAGAACCGAAGCAAAAGTTGGTTCGGATAAAGATACATTTGTCAGGAACTACATGCAGCGTGTTATTGGATTCAATGACTCGAACGTCATGGAAATAGGCCCCCTTTACAACGTGGACGAGTTCAAGAGCAAACGTATATCTGCTGCCTTTCTTGAACTCCCATATGCAAAAGTATTCATGAATCAGAACTGTAAAGGATATACTCAAACCGCGCCAACCTACAGATTCGGAGGATTCAGCTTTGCATTCCGGAAAGGTTCTCCAATAGCGAGGGATTTCACTAGGGTCATTCTAGAGCTATTGGAAAATGGAAAACTGAAGGAGCTAGAAAATAAGTGGTTGACTACAAGGGAAGAGTGTTCAAGAAATGCGACTTCAGATACCCCGAAGAGCTTGAACCTCAAGAATTTCACCGGCCTCTATATAATAACCGGCGTTACTTCTACATTTTGTTTTCTAATCTCTCTTTTTATCTTGCTGAGGAAGTTGTGTTGGCGTGAGTCAAACTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

849

Amino Acids

95.47

Weight (kDa)

8.37

Isoelectric Point (pI)

35.63

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	48 - 348	3.7e-18	Periplasmic binding protein
ANF_receptor	PF01094	53 - 392	9.4e-43	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	454 - 561	1.6e-08	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	521 - 793	4.9e-11	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	575 - 824	2e-28	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000350)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g04591 FvH4_1g04600 FvH4_3g08210 FvH4_3g08210 FvH4_3g08211 FvH4_3g32960 FvH4_3g32961 FvH4_5g21700 FvH4_6g22211
malus_domestica	MD05G1305700.v1.1 MD05G1305800.v1.1 MD05G1305900.v1.1 MD05G1306400.v1.1
prunus_persica	Prupe.4G056200_v2.0.a1 Prupe.4G056200_v2.0.a1 Prupe.4G056600_v2.0.a1 Prupe.4G056600_v2.0.a1
pyrus_communis	pycom05g28300 pycom05g28310 pycom05g28350 pycom10g23860
rosa_chinensis	RchiOBHm_Chr5g0009861 RchiOBHm_Chr5g0009881 RchiOBHm_Chr5g0009931 RchiOBHm_Chr5g0009951 RchiOBHm_Chr5g0009981 RchiOBHm_Chr5g0055531 RchiOBHm_Chr5g0055541
rosa_laevigata	RLG00000015779 RLG00000031705 RLG00000031706 RLG00000031711 RLG00000031713 RLG00000031715 RLG00000031716 RLG00000034996
rosa_multiflora	Rmu_co8083594.1_g000001 Rmu_co8206032.1_g000001 Rmu_co8356045.1_g000001 Rmu_sc0000773.1_g000002 Rmu_sc0001709.1_g000001 Rmu_sc0001709.1_g000007 Rmu_sc0001709.1_g000012 Rmu_sc0001709.1_g000013 Rmu_sc0001709.1_g000018 Rmu_sc0001709.1_g000019 Rmu_sc0002275.1_g000004 Rmu_sc0003071.1_g000002 Rmu_sc0003071.1_g000003 Rmu_sc0003630.1_g000014 Rmu_sc0003630.1_g000017 Rmu_sc0003630.1_g000043 Rmu_sc0003630.1_g000044 Rmu_sc0004626.1_g000007
rosa_roxburghii	Rroxscaffold_1G00024770 Rroxscaffold_1G00066180 Rroxscaffold_1G00066200 Rroxscaffold_1G00066210 Rroxscaffold_1G00066240 Rroxscaffold_1G00066250 Rroxscaffold_1G00066320 Rroxscaffold_1G00066340 Rroxscaffold_3G00234120
rosa_rugosa	Rorug04G0446400 Rorug04G0446500 Rorug04G0446600 Rorug04G0447000 Rorug04G0447100 Rorug04G0447200 Rorug04G0447300 Rorug04G0447900 Rorug04G0448200
rosa_samantha	Rh2CG021600 Rh2DG021300 Rh4AG195700 Rh4BG194700 Rh5AG077900 Rh5AG078100 Rh5AG078600 Rh5AG078700 Rh5AG078900 Rh5AG079000 Rh5AG079100 Rh5AG363100 Rh5CG085300 Rh5CG085900 Rh5CG086000 Rh5CG086200 Rh5CG086400 Rh5CG086500 Rh5CG397300 Rh5DG073400 Rh5DG074000 Rh5DG074100 Rh5DG074300 Rh5DG074500 Rh5DG074600 Rh5DG129000 Rh5DG129400 Rh5DG388500
rosa_wichuraiana	Rw2G001690 Rw5G007180 Rw5G007190 Rw5G007230 Rw5G007240 Rw5G007250 Rw5G007320 Rw5G034180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2117
AccB7I	CCANNNNNTGG	1 cut(s)	1811
AccI	GTMKAC	4 cut(s)	51, 294, 519, 1697
AccII	CGCG	3 cut(s)	622, 1381, 2246
AccIII	TCCGGA	1 cut(s)	2283
AciI	CCGC	8 cut(s)	267, 401, 418, 476, 620, 1381, 1739, 2244
AclWI	GGATC	2 cut(s)	1169, 1939
AcoI	YGGCCR	2 cut(s)	136, 422
AcsI	RAATTY	6 cut(s)	963, 1326, 1403, 1447, 1808, 2440
AcuI	CTGAAG	4 cut(s)	1046, 2051, 2372, 2394
AfaI	GTAC	4 cut(s)	494, 1118, 1137, 1492
AfiI	CCNNNNNNNGG	4 cut(s)	23, 959, 1811, 1995
AgeI	ACCGGT	1 cut(s)	1097
AhlI	ACTAGT	3 cut(s)	887, 1025, 1049
AjiI	CACGTC	1 cut(s)	298
AjnI	CCWGG	2 cut(s)	958, 1336
AjuI	GAANNNNNNNTTGG	2 cut(s)	2034, 2066
Alw21I	GWGCWC	2 cut(s)	45, 606
Alw26I	GTCTC	6 cut(s)	225, 237, 308, 752, 771, 1180
AlwI	GGATC	2 cut(s)	1169, 1939
AlwNI	CAGNNNCTG	1 cut(s)	803
Ama87I	CYCGRG	1 cut(s)	1265
Aor13HI	TCCGGA	1 cut(s)	2283
AoxI	GGCC	6 cut(s)	136, 422, 1262, 1792, 2131, 2449
ApeKI	GCWGC	6 cut(s)	176, 257, 329, 1999, 2089, 2177
ApoI	RAATTY	6 cut(s)	963, 1326, 1403, 1447, 1808, 2440
AseI	ATTAAT	1 cut(s)	126
AsiGI	ACCGGT	1 cut(s)	1097
Asp700I	GAANNNNTTC	2 cut(s)	1403, 1718
AspLEI	GCGC	2 cut(s)	417, 2248
AspS9I	GGNCC	5 cut(s)	27, 1263, 1516, 1793, 2132
AsuC2I	CCSGG	2 cut(s)	1266, 1267
AsuHPI	GGTGA	8 cut(s)	97, 382, 388, 607, 1142, 1607, 1826, 2437
AsuII	TTCGAA	2 cut(s)	200, 918
AsuNHI	GCTAGC	2 cut(s)	556, 1609
AvaI	CYCGRG	1 cut(s)	1265
AvaII	GGWCC	2 cut(s)	27, 1516
BaeGI	GKGCMC	1 cut(s)	1285
BaeI	ACNNNNGTAYC	2 cut(s)	2058, 2091
BalI	TGGCCA	1 cut(s)	424
BanII	GRGCYC	1 cut(s)	1319
BbsI	GAAGAC	3 cut(s)	209, 872, 1580
Bbv12I	GWGCWC	2 cut(s)	45, 606
BbvCI	CCTCAGC	1 cut(s)	2513
BbvI	GCAGC	6 cut(s)	188, 244, 341, 1986, 2101, 2164
BccI	CCATC	7 cut(s)	524, 647, 1172, 1403, 1428, 1811, 1997
BceAI	ACGGC	2 cut(s)	123, 2012
BciT130I	CCWGG	2 cut(s)	960, 1338
BciVI	GTATCC	1 cut(s)	1431
BclI	TGATCA	1 cut(s)	343
BcnI	CCSGG	2 cut(s)	1266, 1267
BcoDI	GTCTC	6 cut(s)	225, 237, 308, 752, 771, 1180
BcuI	ACTAGT	3 cut(s)	887, 1025, 1049
BfaI	CTAG	9 cut(s)	557, 888, 1026, 1050, 1610, 1896, 2316, 2327, 2360
BfmI	CTRYAG	2 cut(s)	726, 2254
BfoI	RGCGCY	1 cut(s)	418
BfuI	GTATCC	1 cut(s)	1431
BisI	GCNGC	8 cut(s)	177, 258, 267, 330, 418, 2000, 2090, 2178
BlsI	GCNGC	8 cut(s)	178, 259, 268, 331, 419, 2001, 2091, 2179
Bme1390I	CCNGG	4 cut(s)	960, 1266, 1267, 1338
Bme18I	GGWCC	2 cut(s)	27, 1516
BmeT110I	CYCGRG	1 cut(s)	1265
BmgBI	CACGTC	1 cut(s)	298
BmgT120I	GGNCC	5 cut(s)	27, 1263, 1516, 1793, 2132
BmiI	GGNNCC	4 cut(s)	28, 1335, 1784, 2134
BmrFI	CCNGG	4 cut(s)	960, 1266, 1267, 1338
BmsI	GCATC	5 cut(s)	392, 543, 657, 1474, 1573
BmtI	GCTAGC	2 cut(s)	560, 1613
BpiI	GAAGAC	3 cut(s)	209, 872, 1580
BplI	GAGNNNNNCTC	2 cut(s)	1877, 1909
Bpu10I	CCTNAGC	2 cut(s)	1949, 2513
Bpu14I	TTCGAA	2 cut(s)	200, 918
BpuEI	CTTGAG	3 cut(s)	318, 1939, 2420
BpuMI	CCSGG	2 cut(s)	1266, 1267
BsaBI	GATNNNNATC	1 cut(s)	995
BsaI	GGTCTC	1 cut(s)	237
BsaJI	CCNNGG	4 cut(s)	73, 165, 1225, 1265
BsaWI	WCCGGW	2 cut(s)	1097, 2283
BsaXI	ACNNNNNCTCC	4 cut(s)	19, 49, 1119, 1149
Bsc4I	CCNNNNNNNGG	4 cut(s)	23, 959, 1811, 1995
Bse118I	RCCGGY	3 cut(s)	1097, 2447, 2465
Bse1I	ACTGG	1 cut(s)	1518
Bse3DI	GCAATG	1 cut(s)	106
Bse8I	GATNNNNATC	1 cut(s)	995
BseAI	TCCGGA	1 cut(s)	2283
BseBI	CCWGG	2 cut(s)	960, 1338
BseDI	CCNNGG	4 cut(s)	73, 165, 1225, 1265
BseGI	GGATG	2 cut(s)	558, 658
BseJI	GATNNNNATC	1 cut(s)	995
BseLI	CCNNNNNNNGG	4 cut(s)	23, 959, 1811, 1995
BseMI	GCAATG	1 cut(s)	106
BseMII	CTCAG	5 cut(s)	561, 795, 1655, 1892, 2504
BseNI	ACTGG	1 cut(s)	1518
BseRI	GAGGAG	3 cut(s)	1706, 1775, 1852
BseSI	GKGCMC	1 cut(s)	1285
BseXI	GCAGC	6 cut(s)	188, 244, 341, 1986, 2101, 2164
Bsh1236I	CGCG	3 cut(s)	622, 1381, 2246
BshFI	GGCC	6 cut(s)	138, 424, 1264, 1794, 2133, 2451
BshTI	ACCGGT	1 cut(s)	1097
BsiHKAI	GWGCWC	2 cut(s)	45, 606
BsiHKCI	CYCGRG	1 cut(s)	1265
BsiSI	CCGG	5 cut(s)	1098, 1266, 2284, 2448, 2466
BslFI	GGGAC	2 cut(s)	40, 777
BslI	CCNNNNNNNGG	4 cut(s)	23, 959, 1811, 1995
BsmAI	GTCTC	6 cut(s)	225, 237, 308, 752, 771, 1180
BsmBI	CGTCTC	1 cut(s)	752
BsmFI	GGGAC	2 cut(s)	40, 777
BsmI	GAATGC	1 cut(s)	2279
BsnI	GGCC	6 cut(s)	138, 424, 1264, 1794, 2133, 2451
Bso31I	GGTCTC	1 cut(s)	237
BsoBI	CYCGRG	1 cut(s)	1265
Bsp119I	TTCGAA	2 cut(s)	200, 918
Bsp1286I	GDGCHC	4 cut(s)	45, 606, 1285, 1319
Bsp13I	TCCGGA	1 cut(s)	2283
Bsp143I	GATC	5 cut(s)	343, 1161, 1590, 1615, 1944
Bsp19I	CCATGG	2 cut(s)	73, 165
BspACI	CCGC	8 cut(s)	267, 401, 418, 476, 620, 1381, 1739, 2244
BspANI	GGCC	6 cut(s)	138, 424, 1264, 1794, 2133, 2451
BspCNI	CTCAG	5 cut(s)	562, 794, 1654, 1891, 2505
BspEI	TCCGGA	1 cut(s)	2283
BspFNI	CGCG	3 cut(s)	622, 1381, 2246
BspHI	TCATGA	2 cut(s)	1468, 2211
BspLI	GGNNCC	4 cut(s)	28, 1335, 1784, 2134
BspOI	GCTAGC	2 cut(s)	560, 1613
BspPI	GGATC	2 cut(s)	1169, 1939
BspQI	GCTCTTC	2 cut(s)	582, 2417
BspT104I	TTCGAA	2 cut(s)	200, 918
BspTNI	GGTCTC	1 cut(s)	237
BsrDI	GCAATG	1 cut(s)	106
BsrFI	RCCGGY	3 cut(s)	1097, 2447, 2465
BsrI	ACTGG	1 cut(s)	1518
BssAI	RCCGGY	3 cut(s)	1097, 2447, 2465
BssECI	CCNNGG	4 cut(s)	73, 165, 1225, 1265
BssMI	GATC	5 cut(s)	343, 1161, 1590, 1615, 1944
BssNAI	GTATAC	1 cut(s)	520
BssT1I	CCWWGG	3 cut(s)	73, 165, 1225
Bst1107I	GTATAC	1 cut(s)	520
Bst2UI	CCWGG	2 cut(s)	960, 1338
Bst4CI	ACNGT	8 cut(s)	370, 452, 539, 608, 730, 1037, 1606, 2225
Bst6I	CTCTTC	7 cut(s)	582, 657, 769, 1178, 2027, 2382, 2417
BstBI	TTCGAA	2 cut(s)	200, 918
BstC8I	GCNNGC	3 cut(s)	422, 558, 1611
BstDEI	CTNAG	7 cut(s)	570, 781, 1103, 1641, 1878, 1949, 2513
BstDSI	CCRYGG	2 cut(s)	73, 165
BstF5I	GGATG	2 cut(s)	558, 658
BstFNI	CGCG	3 cut(s)	622, 1381, 2246
BstH2I	RGCGCY	1 cut(s)	418
BstHHI	GCGC	2 cut(s)	417, 2248
BstKTI	GATC	5 cut(s)	346, 1164, 1593, 1618, 1947
BstMAI	GTCTC	6 cut(s)	225, 237, 308, 752, 771, 1180
BstMBI	GATC	5 cut(s)	343, 1161, 1590, 1615, 1944
BstMWI	GCNNNNNNNGC	6 cut(s)	263, 266, 338, 421, 977, 1221
BstNI	CCWGG	2 cut(s)	960, 1338
BstNSI	RCATGY	1 cut(s)	2089
BstSCI	CCNGG	4 cut(s)	958, 1264, 1265, 1336
BstSFI	CTRYAG	2 cut(s)	726, 2254
BstSLI	GKGCMC	1 cut(s)	1285
BstUI	CGCG	3 cut(s)	622, 1381, 2246
BstV1I	GCAGC	6 cut(s)	188, 244, 341, 1986, 2101, 2164
BstV2I	GAAGAC	3 cut(s)	209, 872, 1580
BstXI	CCANNNNNNTGG	1 cut(s)	80
BstZ17I	GTATAC	1 cut(s)	520
BsuI	GTATCC	1 cut(s)	1431
BsuRI	GGCC	6 cut(s)	138, 424, 1264, 1794, 2133, 2451
BtgI	CCRYGG	2 cut(s)	73, 165
BtgZI	GCGATG	1 cut(s)	369
BtrI	CACGTC	1 cut(s)	298
BtsCI	GGATG	2 cut(s)	558, 658
BtsI	GCAGTG	1 cut(s)	1962
BtsIMutI	CAGTG	2 cut(s)	735, 1962
Cac8I	GCNNGC	3 cut(s)	422, 558, 1611
CaiI	CAGNNNCTG	1 cut(s)	803
CciI	TCATGA	2 cut(s)	1468, 2211
CfoI	GCGC	2 cut(s)	417, 2248
Cfr10I	RCCGGY	3 cut(s)	1097, 2447, 2465
Cfr13I	GGNCC	5 cut(s)	27, 1263, 1516, 1793, 2132
Cfr9I	CCCGGG	1 cut(s)	1265
Csp6I	GTAC	4 cut(s)	493, 1117, 1136, 1491
CspAI	ACCGGT	1 cut(s)	1097
CviQI	GTAC	4 cut(s)	493, 1117, 1136, 1491
DdeI	CTNAG	7 cut(s)	570, 781, 1103, 1641, 1878, 1949, 2513
DpnI	GATC	5 cut(s)	345, 1163, 1592, 1617, 1946
DpnII	GATC	5 cut(s)	343, 1161, 1590, 1615, 1944
DrdI	GACNNNNNNGTC	1 cut(s)	2117
DseDI	GACNNNNNNGTC	1 cut(s)	2117
EaeI	YGGCCR	2 cut(s)	136, 422
Eam1104I	CTCTTC	7 cut(s)	582, 657, 769, 1178, 2027, 2382, 2417
EarI	CTCTTC	7 cut(s)	582, 657, 769, 1178, 2027, 2382, 2417
Eco130I	CCWWGG	3 cut(s)	73, 165, 1225
Eco24I	GRGCYC	1 cut(s)	1319
Eco31I	GGTCTC	1 cut(s)	237
Eco47I	GGWCC	2 cut(s)	27, 1516
Eco57I	CTGAAG	4 cut(s)	1046, 2051, 2372, 2394
Eco88I	CYCGRG	1 cut(s)	1265
EcoO109I	RGGNCCY	2 cut(s)	27, 2132
EcoRII	CCWGG	2 cut(s)	958, 1336
EcoT14I	CCWWGG	3 cut(s)	73, 165, 1225
EcoT38I	GRGCYC	1 cut(s)	1319
ErhI	CCWWGG	3 cut(s)	73, 165, 1225
Esp3I	CGTCTC	1 cut(s)	752
FalI	AAGNNNNNCTT	2 cut(s)	1619, 1651
FaqI	GGGAC	2 cut(s)	40, 777
FauI	CCCGC	2 cut(s)	408, 627
FauNDI	CATATG	2 cut(s)	1579, 2197
FbaI	TGATCA	1 cut(s)	343
FblI	GTMKAC	4 cut(s)	51, 294, 519, 1697
Fnu4HI	GCNGC	8 cut(s)	177, 258, 267, 330, 418, 2000, 2090, 2178
FokI	GGATG	2 cut(s)	565, 665
FriOI	GRGCYC	1 cut(s)	1319
Fsp4HI	GCNGC	8 cut(s)	177, 258, 267, 330, 418, 2000, 2090, 2178
FspBI	CTAG	9 cut(s)	557, 888, 1026, 1050, 1610, 1896, 2316, 2327, 2360
GlaI	GCGC	2 cut(s)	416, 2247
GluI	GCNGC	8 cut(s)	177, 258, 267, 330, 418, 2000, 2090, 2178
HaeII	RGCGCY	1 cut(s)	418
HaeIII	GGCC	6 cut(s)	138, 424, 1264, 1794, 2133, 2451
HapII	CCGG	5 cut(s)	1098, 1266, 2284, 2448, 2466
HhaI	GCGC	2 cut(s)	417, 2248
Hin6I	GCGC	2 cut(s)	415, 2246
HinP1I	GCGC	2 cut(s)	415, 2246
HincII	GTYRAC	2 cut(s)	1698, 2376
HindII	GTYRAC	2 cut(s)	1698, 2376
HpaII	CCGG	5 cut(s)	1098, 1266, 2284, 2448, 2466
HphI	GGTGA	8 cut(s)	97, 382, 388, 607, 1142, 1607, 1826, 2437
Hpy166II	GTNNAC	8 cut(s)	52, 295, 520, 1633, 1698, 1854, 2149, 2376
Hpy8I	GTNNAC	8 cut(s)	52, 295, 520, 1633, 1698, 1854, 2149, 2376
HpyAV	CCTTC	9 cut(s)	40, 230, 283, 748, 842, 1070, 1360, 1784, 2347
HpyCH4III	ACNGT	8 cut(s)	370, 452, 539, 608, 730, 1037, 1606, 2225
HpyCH4IV	ACGT	6 cut(s)	297, 1534, 2010, 2118, 2145, 2167
HpyCH4V	TGCA	9 cut(s)	176, 971, 1122, 1419, 1465, 2002, 2089, 2201, 2279
HpyF10VI	GCNNNNNNNGC	6 cut(s)	263, 266, 338, 421, 977, 1221
HpyF3I	CTNAG	7 cut(s)	570, 781, 1103, 1641, 1878, 1949, 2513
HpySE526I	ACGT	6 cut(s)	297, 1534, 2010, 2118, 2145, 2167
HspAI	GCGC	2 cut(s)	415, 2246
Kpn2I	TCCGGA	1 cut(s)	2283
Ksp22I	TGATCA	1 cut(s)	343
Kzo9I	GATC	5 cut(s)	343, 1161, 1590, 1615, 1944
LguI	GCTCTTC	2 cut(s)	582, 2417
LmnI	GCTCC	6 cut(s)	40, 83, 116, 542, 1788, 2356
Lsp1109I	GCAGC	6 cut(s)	188, 244, 341, 1986, 2101, 2164
LweI	GCATC	5 cut(s)	392, 543, 657, 1474, 1573
MaeI	CTAG	9 cut(s)	557, 888, 1026, 1050, 1610, 1896, 2316, 2327, 2360
MaeII	ACGT	6 cut(s)	297, 1534, 2010, 2118, 2145, 2167
MaeIII	GTNAC	7 cut(s)	103, 364, 533, 730, 1028, 2011, 2470
MalI	GATC	5 cut(s)	345, 1163, 1592, 1617, 1946
MboI	GATC	5 cut(s)	343, 1161, 1590, 1615, 1944
MhlI	GDGCHC	4 cut(s)	45, 606, 1285, 1319
MlsI	TGGCCA	1 cut(s)	424
MluNI	TGGCCA	1 cut(s)	424
MlyI	GAGTC	9 cut(s)	199, 227, 727, 773, 818, 1664, 1703, 2105, 2543
MmeI	TCCRAC	3 cut(s)	61, 1175, 1602
Mox20I	TGGCCA	1 cut(s)	424
MroI	TCCGGA	1 cut(s)	2283
MroXI	GAANNNNTTC	2 cut(s)	1403, 1718
MscI	TGGCCA	1 cut(s)	424
MseI	TTAA	4 cut(s)	126, 464, 1073, 1484
MslI	CAYNNNNRTG	1 cut(s)	2024
Msp20I	TGGCCA	1 cut(s)	424
MspA1I	CMGCKG	2 cut(s)	179, 420
MspI	CCGG	5 cut(s)	1098, 1266, 2284, 2448, 2466
MspR9I	CCNGG	4 cut(s)	960, 1266, 1267, 1338
Mva1269I	GAATGC	1 cut(s)	2279
MvaI	CCWGG	2 cut(s)	960, 1338
MvnI	CGCG	3 cut(s)	622, 1381, 2246
MwoI	GCNNNNNNNGC	6 cut(s)	263, 266, 338, 421, 977, 1221
NciI	CCSGG	2 cut(s)	1266, 1267
NcoI	CCATGG	2 cut(s)	73, 165
NdeI	CATATG	2 cut(s)	1579, 2197
NdeII	GATC	5 cut(s)	343, 1161, 1590, 1615, 1944
NheI	GCTAGC	2 cut(s)	556, 1609
NlaIV	GGNNCC	4 cut(s)	28, 1335, 1784, 2134
NmuCI	GTSAC	2 cut(s)	103, 730
NspI	RCATGY	1 cut(s)	2089
NspV	TTCGAA	2 cut(s)	200, 918
PagI	TCATGA	2 cut(s)	1468, 2211
PciSI	GCTCTTC	2 cut(s)	582, 2417
PctI	GAATGC	1 cut(s)	2279
PdmI	GAANNNNTTC	2 cut(s)	1403, 1718
PfeI	GAWTC	7 cut(s)	613, 1499, 1828, 2103, 2215, 2259, 2268
PflMI	CCANNNNNTGG	1 cut(s)	1811
PinAI	ACCGGT	1 cut(s)	1097
PkrI	GCNGC	8 cut(s)	178, 259, 268, 331, 419, 2001, 2091, 2179
PleI	GAGTC	9 cut(s)	199, 227, 727, 773, 818, 1663, 1702, 2105, 2542
PpsI	GAGTC	9 cut(s)	199, 227, 727, 773, 818, 1663, 1702, 2105, 2542
PpuMI	RGGWCCY	1 cut(s)	27
PshBI	ATTAAT	1 cut(s)	126
Psp5II	RGGWCCY	1 cut(s)	27
Psp6I	CCWGG	2 cut(s)	958, 1336
PspGI	CCWGG	2 cut(s)	958, 1336
PspN4I	GGNNCC	4 cut(s)	28, 1335, 1784, 2134
PspPI	GGNCC	5 cut(s)	27, 1263, 1516, 1793, 2132
PspPPI	RGGWCCY	1 cut(s)	27
PsrI	GAACNNNNNNTAC	2 cut(s)	1021, 1053
PstNI	CAGNNNCTG	1 cut(s)	803
PvuII	CAGCTG	1 cut(s)	179
RsaI	GTAC	4 cut(s)	494, 1118, 1137, 1492
RsaNI	GTAC	4 cut(s)	493, 1117, 1136, 1491
RseI	CAYNNNNRTG	1 cut(s)	2024
SalI	GTCGAC	1 cut(s)	1696
SapI	GCTCTTC	2 cut(s)	582, 2417
SaqAI	TTAA	4 cut(s)	126, 464, 1073, 1484
SatI	GCNGC	8 cut(s)	177, 258, 267, 330, 418, 2000, 2090, 2178
Sau3AI	GATC	5 cut(s)	343, 1161, 1590, 1615, 1944
Sau96I	GGNCC	5 cut(s)	27, 1263, 1516, 1793, 2132
SchI	GAGTC	9 cut(s)	199, 227, 727, 773, 818, 1664, 1703, 2105, 2543
ScrFI	CCNGG	4 cut(s)	960, 1266, 1267, 1338
SduI	GDGCHC	4 cut(s)	45, 606, 1285, 1319
SfaNI	GCATC	5 cut(s)	392, 543, 657, 1474, 1573
SfcI	CTRYAG	2 cut(s)	726, 2254
SfuI	TTCGAA	2 cut(s)	200, 918
SinI	GGWCC	2 cut(s)	27, 1516
SmaI	CCCGGG	1 cut(s)	1267
SmiMI	CAYNNNNRTG	1 cut(s)	2024
SmlI	CTYRAG	3 cut(s)	333, 1954, 2435
SmoI	CTYRAG	3 cut(s)	333, 1954, 2435
SpeI	ACTAGT	3 cut(s)	887, 1025, 1049
SsiI	CCGC	8 cut(s)	267, 401, 418, 476, 620, 1381, 1739, 2244
SspMI	CTAG	9 cut(s)	557, 888, 1026, 1050, 1610, 1896, 2316, 2327, 2360
StyD4I	CCNGG	4 cut(s)	958, 1264, 1265, 1336
StyI	CCWWGG	3 cut(s)	73, 165, 1225
TaaI	ACNGT	8 cut(s)	370, 452, 539, 608, 730, 1037, 1606, 2225
TaiI	ACGT	6 cut(s)	300, 1537, 2013, 2121, 2148, 2170
TatI	WGTACW	2 cut(s)	1116, 1490
TauI	GCSGC	2 cut(s)	269, 420
TfiI	GAWTC	7 cut(s)	613, 1499, 1828, 2103, 2215, 2259, 2268
Tru1I	TTAA	4 cut(s)	126, 464, 1073, 1484
Tru9I	TTAA	4 cut(s)	126, 464, 1073, 1484
TscAI	CASTG	2 cut(s)	735, 1969
TseFI	GTSAC	2 cut(s)	103, 730
TseI	GCWGC	6 cut(s)	176, 257, 329, 1999, 2089, 2177
Tsp45I	GTSAC	2 cut(s)	103, 730
TspGWI	ACGGA	2 cut(s)	1251, 2030
TspMI	CCCGGG	1 cut(s)	1265
TspRI	CASTG	2 cut(s)	735, 1969
Van91I	CCANNNNNTGG	1 cut(s)	1811
VpaK11BI	GGWCC	2 cut(s)	27, 1516
VspI	ATTAAT	1 cut(s)	126
XapI	RAATTY	6 cut(s)	963, 1326, 1403, 1447, 1808, 2440
XbaI	TCTAGA	1 cut(s)	2326
XceI	RCATGY	1 cut(s)	2089
XmaI	CCCGGG	1 cut(s)	1265
XmiI	GTMKAC	4 cut(s)	51, 294, 519, 1697
XmnI	GAANNNNTTC	2 cut(s)	1403, 1718
XspI	CTAG	9 cut(s)	557, 888, 1026, 1050, 1610, 1896, 2316, 2327, 2360

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.