Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000350

Physical Location & Seq

Genomic Coordinates Forward (+)

43816482 .. 43817905

Gene Structure 1424 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1305800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1221 bp

ATGCTAGCTCTTTTGACCGAGGCCCTTCAAGATGTCGGTTCAAAGATTGAGCACCGTTTGGTTCTTCCGCAGATTTCTTCGCTGTCTAATCCAAACGGGGTCGAGCTAGAAGAGATGTTGAGGCTTCCCACCATGCATTCTCGGTTGTTTATCATTCTCCAGTCATCGTTGCCTACGGTGACTAATCTATTTCGATTGGCTGGAAAGATGGGACTTGTTGGAAAAGAGTCAGCTTGGATCATCACCGAGAGTATTGCAAGTTTGCTTGACCCTCAACACACCTCTGACATGAAAGGCACTCTGGGAATCAAGACCTACTATGCCAATAATACTAGTTCTTATACAAATTTCCGGAAGAAATTCCAAACGAAGTATTCAGAAGGAAATAGCGCCCAGCCAGGAATTTACGCTCTTCGAGCATATGATGCTATTGGAATCATTTCACAGGCCATAGGAAGAATGACTGATAACACAACTAGCCTTGAGGTGTTGAAAACCGTATTGAGCAGTTATACAGAATTGAACTATTGGAAACCGGAAGTTGGGTTCTCATCGGATGAAGCAGGGAAATACAGATTCAGTGATGTTGGTTTAGTAATCTGGCCCGGGAACCTAGCTAGTGCCCCGAAAGGCTGGGCAATGCCTACTGTTGAGAAGCCAATGAGAATTGGGGTGCCGGGTAAAACTTCCTTCAGCAACTTTGTGAAGGTTGATCGGTCCAGGCAAGGCTCAGATAAGAAGAAGTATGTCGGTTTCTGCATTGCAATTTTTGATATGGTCATAAATCGTTTGAACTATTCTCTGCCGTATCAATTTGAAGTTTTCGATGGCTCGTACGATCATCTTGTGGAACAAGTCCATAAAAAGGTTTATGATGCTGTGGTTGGTTACATAACTGTGCTAGCTGACCGGCTGGACAAGGTGGAATTCACTCAGCCATATATGGAGTCTGGTTTGTCAATGATAGTTCCGGCAGAACCCAAAAAATCAACATGGATGTTTATGAAACCTTTCACATGGCAAATGTGGGTGGTGACTGGTTCCATCTTAATCTACACAGTGTTCATCGTATGGTTCCTGGAGCGCCCGTCAAATCCAGAATTTGGTGGCCCCTTGAAGAATCAGATTGGCACAGCAACTTGGTTCACCTTCTCCTCTTTGTTCTTTGCTCACAGTAAGTACCAATTTTGTACATTTCACTACCAAAAAAGTGAGTATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

407

Amino Acids

45.99

Weight (kDa)

8.86

Isoelectric Point (pI)

31.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANF_receptor	PF01094	3 - 157	4.2e-12	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	234 - 324	7.5e-08	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	245 - 329	9.2e-09	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	339 - 391	4.8e-06	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000350)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g04591 FvH4_1g04600 FvH4_3g08210 FvH4_3g08210 FvH4_3g08211 FvH4_3g32960 FvH4_3g32961 FvH4_5g21700 FvH4_6g22211
malus_domestica	MD05G1305700.v1.1 MD05G1305800.v1.1 MD05G1305900.v1.1 MD05G1306400.v1.1
prunus_persica	Prupe.4G056200_v2.0.a1 Prupe.4G056200_v2.0.a1 Prupe.4G056600_v2.0.a1 Prupe.4G056600_v2.0.a1
pyrus_communis	pycom05g28300 pycom05g28310 pycom05g28350 pycom10g23860
rosa_chinensis	RchiOBHm_Chr5g0009861 RchiOBHm_Chr5g0009881 RchiOBHm_Chr5g0009931 RchiOBHm_Chr5g0009951 RchiOBHm_Chr5g0009981 RchiOBHm_Chr5g0055531 RchiOBHm_Chr5g0055541
rosa_laevigata	RLG00000015779 RLG00000031705 RLG00000031706 RLG00000031711 RLG00000031713 RLG00000031715 RLG00000031716 RLG00000034996
rosa_multiflora	Rmu_co8083594.1_g000001 Rmu_co8206032.1_g000001 Rmu_co8356045.1_g000001 Rmu_sc0000773.1_g000002 Rmu_sc0001709.1_g000001 Rmu_sc0001709.1_g000007 Rmu_sc0001709.1_g000012 Rmu_sc0001709.1_g000013 Rmu_sc0001709.1_g000018 Rmu_sc0001709.1_g000019 Rmu_sc0002275.1_g000004 Rmu_sc0003071.1_g000002 Rmu_sc0003071.1_g000003 Rmu_sc0003630.1_g000014 Rmu_sc0003630.1_g000017 Rmu_sc0003630.1_g000043 Rmu_sc0003630.1_g000044 Rmu_sc0004626.1_g000007
rosa_roxburghii	Rroxscaffold_1G00024770 Rroxscaffold_1G00066180 Rroxscaffold_1G00066200 Rroxscaffold_1G00066210 Rroxscaffold_1G00066240 Rroxscaffold_1G00066250 Rroxscaffold_1G00066320 Rroxscaffold_1G00066340 Rroxscaffold_3G00234120
rosa_rugosa	Rorug04G0446400 Rorug04G0446500 Rorug04G0446600 Rorug04G0447000 Rorug04G0447100 Rorug04G0447200 Rorug04G0447300 Rorug04G0447900 Rorug04G0448200
rosa_samantha	Rh2CG021600 Rh2DG021300 Rh4AG195700 Rh4BG194700 Rh5AG077900 Rh5AG078100 Rh5AG078600 Rh5AG078700 Rh5AG078900 Rh5AG079000 Rh5AG079100 Rh5AG363100 Rh5CG085300 Rh5CG085900 Rh5CG086000 Rh5CG086200 Rh5CG086400 Rh5CG086500 Rh5CG397300 Rh5DG073400 Rh5DG074000 Rh5DG074100 Rh5DG074300 Rh5DG074500 Rh5DG074600 Rh5DG129000 Rh5DG129400 Rh5DG388500
rosa_wichuraiana	Rw2G001690 Rw5G007180 Rw5G007190 Rw5G007230 Rw5G007240 Rw5G007250 Rw5G007320 Rw5G034180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	673
AccB7I	CCANNNNNTGG	1 cut(s)	1103
AccIII	TCCGGA	1 cut(s)	351
AciI	CCGC	1 cut(s)	68
AclWI	GGATC	1 cut(s)	245
AcsI	RAATTY	5 cut(s)	346, 359, 402, 926, 1100
AcuI	CTGAAG	1 cut(s)	676
AfaI	GTAC	3 cut(s)	836, 1181, 1192
AfiI	CCNNNNNNNGG	3 cut(s)	542, 865, 1103
AgsI	TTSAA	7 cut(s)	29, 42, 493, 523, 793, 818, 1117
AhlI	ACTAGT	1 cut(s)	332
AjnI	CCWGG	3 cut(s)	397, 719, 1077
AloI	GAACNNNNNNTCC	2 cut(s)	530, 562
AluBI	AGCT	5 cut(s)	8, 106, 233, 617, 905
AluI	AGCT	5 cut(s)	8, 106, 233, 617, 905
Alw21I	GWGCWC	1 cut(s)	54
AlwI	GGATC	1 cut(s)	245
Ama87I	CYCGRG	1 cut(s)	605
Aor13HI	TCCGGA	1 cut(s)	351
AoxI	GGCC	4 cut(s)	21, 447, 602, 1108
ApoI	RAATTY	5 cut(s)	346, 359, 402, 926, 1100
Asp700I	GAANNNNTTC	3 cut(s)	359, 439, 1010
AspLEI	GCGC	2 cut(s)	392, 1086
AspS9I	GGNCC	4 cut(s)	22, 603, 717, 1109
AsuC2I	CCSGG	3 cut(s)	606, 607, 678
AsuHPI	GGTGA	4 cut(s)	190, 235, 1045, 1138
AsuNHI	GCTAGC	2 cut(s)	4, 901
AvaI	CYCGRG	1 cut(s)	605
AvaII	GGWCC	1 cut(s)	717
BaeGI	GKGCMC	1 cut(s)	625
BanI	GGYRCC	1 cut(s)	673
Bbv12I	GWGCWC	1 cut(s)	54
BccI	CCATC	3 cut(s)	202, 821, 1052
BceAI	ACGGC	1 cut(s)	790
BcgI	CGANNNNNNTGC	2 cut(s)	236, 270
BciT130I	CCWGG	3 cut(s)	399, 721, 1079
BcnI	CCSGG	3 cut(s)	606, 607, 678
BcuI	ACTAGT	1 cut(s)	332
BfaI	CTAG	7 cut(s)	5, 107, 333, 477, 614, 618, 902
BfoI	RGCGCY	2 cut(s)	393, 1087
Bme1390I	CCNGG	6 cut(s)	399, 606, 607, 678, 721, 1079
Bme18I	GGWCC	1 cut(s)	717
BmeT110I	CYCGRG	1 cut(s)	605
BmgT120I	GGNCC	4 cut(s)	22, 603, 717, 1109
BmiI	GGNNCC	5 cut(s)	611, 675, 1042, 1076, 1111
BmrFI	CCNGG	6 cut(s)	399, 606, 607, 678, 721, 1079
BmsI	GCATC	2 cut(s)	415, 865
BmtI	GCTAGC	2 cut(s)	8, 905
BpmI	CTGGAG	2 cut(s)	143, 1100
BpuEI	CTTGAG	1 cut(s)	503
BpuMI	CCSGG	3 cut(s)	606, 607, 678
BsaJI	CCNNGG	2 cut(s)	18, 605
BsaWI	WCCGGW	2 cut(s)	351, 535
BsaXI	ACNNNNNCTCC	2 cut(s)	1073, 1103
Bsc4I	CCNNNNNNNGG	3 cut(s)	542, 865, 1103
Bse118I	RCCGGY	1 cut(s)	909
Bse1I	ACTGG	2 cut(s)	160, 1042
Bse3DI	GCAATG	2 cut(s)	645, 759
BseAI	TCCGGA	1 cut(s)	351
BseBI	CCWGG	3 cut(s)	399, 721, 1079
BseDI	CCNNGG	2 cut(s)	18, 605
BseGI	GGATG	2 cut(s)	562, 1002
BseLI	CCNNNNNNNGG	3 cut(s)	542, 865, 1103
BseMI	GCAATG	2 cut(s)	645, 759
BseMII	CTCAG	2 cut(s)	744, 947
BseNI	ACTGG	2 cut(s)	160, 1042
BseRI	GAGGAG	1 cut(s)	1144
BseSI	GKGCMC	1 cut(s)	625
BseYI	CCCAGC	2 cut(s)	393, 633
BshFI	GGCC	4 cut(s)	23, 449, 604, 1110
BshNI	GGYRCC	1 cut(s)	673
BsiHKAI	GWGCWC	1 cut(s)	54
BsiHKCI	CYCGRG	1 cut(s)	605
BsiSI	CCGG	6 cut(s)	352, 536, 606, 677, 910, 971
BsiWI	CGTACG	1 cut(s)	834
BslFI	GGGAC	1 cut(s)	225
BslI	CCNNNNNNNGG	3 cut(s)	542, 865, 1103
BsmFI	GGGAC	1 cut(s)	225
BsmI	GAATGC	1 cut(s)	136
BsnI	GGCC	4 cut(s)	23, 449, 604, 1110
BsoBI	CYCGRG	1 cut(s)	605
Bsp1286I	GDGCHC	2 cut(s)	54, 625
Bsp13I	TCCGGA	1 cut(s)	351
Bsp1407I	TGTACA	1 cut(s)	1190
Bsp143I	GATC	3 cut(s)	237, 712, 838
BspACI	CCGC	1 cut(s)	68
BspANI	GGCC	4 cut(s)	23, 449, 604, 1110
BspCNI	CTCAG	2 cut(s)	743, 946
BspEI	TCCGGA	1 cut(s)	351
BspLI	GGNNCC	5 cut(s)	611, 675, 1042, 1076, 1111
BspOI	GCTAGC	2 cut(s)	8, 905
BspPI	GGATC	1 cut(s)	245
BspQI	GCTCTTC	1 cut(s)	417
BspT107I	GGYRCC	1 cut(s)	673
BsrDI	GCAATG	2 cut(s)	645, 759
BsrFI	RCCGGY	1 cut(s)	909
BsrGI	TGTACA	1 cut(s)	1190
BsrI	ACTGG	2 cut(s)	160, 1042
BssAI	RCCGGY	1 cut(s)	909
BssECI	CCNNGG	2 cut(s)	18, 605
BssMI	GATC	3 cut(s)	237, 712, 838
Bst2UI	CCWGG	3 cut(s)	399, 721, 1079
Bst4CI	ACNGT	7 cut(s)	56, 178, 499, 649, 898, 1060, 1175
Bst6I	CTCTTC	2 cut(s)	105, 417
BstAPI	GCANNNNNTGC	1 cut(s)	425
BstAUI	TGTACA	1 cut(s)	1190
BstC8I	GCNNGC	2 cut(s)	6, 903
BstDEI	CTNAG	2 cut(s)	730, 933
BstF5I	GGATG	2 cut(s)	562, 1002
BstH2I	RGCGCY	2 cut(s)	393, 1087
BstHHI	GCGC	2 cut(s)	392, 1086
BstKTI	GATC	3 cut(s)	240, 715, 841
BstMBI	GATC	3 cut(s)	237, 712, 838
BstMWI	GCNNNNNNNGC	2 cut(s)	416, 425
BstNI	CCWGG	3 cut(s)	399, 721, 1079
BstSCI	CCNGG	6 cut(s)	397, 604, 605, 676, 719, 1077
BstSLI	GKGCMC	1 cut(s)	625
BsuRI	GGCC	4 cut(s)	23, 449, 604, 1110
BtsCI	GGATG	2 cut(s)	562, 1002
BtsIMutI	CAGTG	2 cut(s)	586, 1065
Cac8I	GCNNGC	2 cut(s)	6, 903
CfoI	GCGC	2 cut(s)	392, 1086
Cfr10I	RCCGGY	1 cut(s)	909
Cfr13I	GGNCC	4 cut(s)	22, 603, 717, 1109
Cfr9I	CCCGGG	1 cut(s)	605
Csp6I	GTAC	3 cut(s)	835, 1180, 1191
CviAII	CATG	4 cut(s)	133, 289, 993, 1017
CviQI	GTAC	3 cut(s)	835, 1180, 1191
DdeI	CTNAG	2 cut(s)	730, 933
DpnI	GATC	3 cut(s)	239, 714, 840
DpnII	GATC	3 cut(s)	237, 712, 838
Eam1104I	CTCTTC	2 cut(s)	105, 417
EarI	CTCTTC	2 cut(s)	105, 417
Eco47I	GGWCC	1 cut(s)	717
Eco57I	CTGAAG	1 cut(s)	676
Eco88I	CYCGRG	1 cut(s)	605
EcoO109I	RGGNCCY	1 cut(s)	22
EcoRI	GAATTC	1 cut(s)	926
EcoRII	CCWGG	3 cut(s)	397, 719, 1077
EcoT22I	ATGCAT	1 cut(s)	138
FaeI	CATG	4 cut(s)	136, 292, 996, 1020
FaqI	GGGAC	1 cut(s)	225
FatI	CATG	4 cut(s)	132, 288, 992, 1016
FauNDI	CATATG	1 cut(s)	421
FokI	GGATG	2 cut(s)	569, 1009
FspBI	CTAG	7 cut(s)	5, 107, 333, 477, 614, 618, 902
GlaI	GCGC	2 cut(s)	391, 1085
GsaI	CCCAGC	2 cut(s)	397, 637
GsuI	CTGGAG	2 cut(s)	143, 1100
HaeII	RGCGCY	2 cut(s)	393, 1087
HaeIII	GGCC	4 cut(s)	23, 449, 604, 1110
HapII	CCGG	6 cut(s)	352, 536, 606, 677, 910, 971
HhaI	GCGC	2 cut(s)	392, 1086
Hin1II	CATG	4 cut(s)	136, 292, 996, 1020
Hin6I	GCGC	2 cut(s)	390, 1084
HinP1I	GCGC	2 cut(s)	390, 1084
HinfI	GANTC	6 cut(s)	227, 306, 435, 576, 947, 1120
HpaII	CCGG	6 cut(s)	352, 536, 606, 677, 910, 971
HphI	GGTGA	4 cut(s)	190, 235, 1045, 1138
Hpy166II	GTNNAC	1 cut(s)	1146
Hpy188I	TCNGA	5 cut(s)	286, 379, 556, 733, 1125
Hpy188III	TCNNGA	4 cut(s)	29, 310, 352, 1097
Hpy8I	GTNNAC	1 cut(s)	1146
HpyAV	CCTTC	5 cut(s)	35, 374, 700, 700, 1159
HpyCH4III	ACNGT	7 cut(s)	56, 178, 499, 649, 898, 1060, 1175
HpyCH4V	TGCA	4 cut(s)	136, 257, 759, 764
HpyF10VI	GCNNNNNNNGC	2 cut(s)	416, 425
HpyF3I	CTNAG	2 cut(s)	730, 933
Hsp92II	CATG	4 cut(s)	136, 292, 996, 1020
HspAI	GCGC	2 cut(s)	390, 1084
Kpn2I	TCCGGA	1 cut(s)	351
Kzo9I	GATC	3 cut(s)	237, 712, 838
LguI	GCTCTTC	1 cut(s)	417
LmnI	GCTCC	1 cut(s)	1081
LweI	GCATC	2 cut(s)	415, 865
MaeI	CTAG	7 cut(s)	5, 107, 333, 477, 614, 618, 902
MaeIII	GTNAC	3 cut(s)	178, 887, 1033
MalI	GATC	3 cut(s)	239, 714, 840
MboI	GATC	3 cut(s)	237, 712, 838
MboII	GAAGA	8 cut(s)	56, 69, 122, 367, 404, 468, 751, 1129
MhlI	GDGCHC	2 cut(s)	54, 625
MlyI	GAGTC	2 cut(s)	236, 956
MmeI	TCCRAC	1 cut(s)	199
MnlI	CCTC	6 cut(s)	13, 114, 282, 292, 478, 1165
Mph1103I	ATGCAT	1 cut(s)	138
MroI	TCCGGA	1 cut(s)	351
MroXI	GAANNNNTTC	3 cut(s)	359, 439, 1010
MseI	TTAA	1 cut(s)	1049
MslI	CAYNNNNRTG	1 cut(s)	896
MspI	CCGG	6 cut(s)	352, 536, 606, 677, 910, 971
MspR9I	CCNGG	6 cut(s)	399, 606, 607, 678, 721, 1079
Mva1269I	GAATGC	1 cut(s)	136
MvaI	CCWGG	3 cut(s)	399, 721, 1079
MwoI	GCNNNNNNNGC	2 cut(s)	416, 425
NciI	CCSGG	3 cut(s)	606, 607, 678
NdeI	CATATG	1 cut(s)	421
NdeII	GATC	3 cut(s)	237, 712, 838
NheI	GCTAGC	2 cut(s)	4, 901
NlaIII	CATG	4 cut(s)	136, 292, 996, 1020
NlaIV	GGNNCC	5 cut(s)	611, 675, 1042, 1076, 1111
NmuCI	GTSAC	2 cut(s)	178, 1033
NsiI	ATGCAT	1 cut(s)	138
PciSI	GCTCTTC	1 cut(s)	417
PctI	GAATGC	1 cut(s)	136
PdmI	GAANNNNTTC	3 cut(s)	359, 439, 1010
PfeI	GAWTC	4 cut(s)	306, 435, 576, 1120
Pfl23II	CGTACG	1 cut(s)	834
PflMI	CCANNNNNTGG	1 cut(s)	1103
PfoI	TCCNGGA	1 cut(s)	1077
PleI	GAGTC	2 cut(s)	235, 955
PpsI	GAGTC	2 cut(s)	235, 955
Psp6I	CCWGG	3 cut(s)	397, 719, 1077
PspFI	CCCAGC	2 cut(s)	393, 633
PspGI	CCWGG	3 cut(s)	397, 719, 1077
PspLI	CGTACG	1 cut(s)	834
PspN4I	GGNNCC	5 cut(s)	611, 675, 1042, 1076, 1111
PspPI	GGNCC	4 cut(s)	22, 603, 717, 1109
RsaI	GTAC	3 cut(s)	836, 1181, 1192
RsaNI	GTAC	3 cut(s)	835, 1180, 1191
RseI	CAYNNNNRTG	1 cut(s)	896
SapI	GCTCTTC	1 cut(s)	417
SaqAI	TTAA	1 cut(s)	1049
Sau3AI	GATC	3 cut(s)	237, 712, 838
Sau96I	GGNCC	4 cut(s)	22, 603, 717, 1109
SchI	GAGTC	2 cut(s)	236, 956
ScrFI	CCNGG	6 cut(s)	399, 606, 607, 678, 721, 1079
SduI	GDGCHC	2 cut(s)	54, 625
SfaNI	GCATC	2 cut(s)	415, 865
SinI	GGWCC	1 cut(s)	717
SmaI	CCCGGG	1 cut(s)	607
SmiMI	CAYNNNNRTG	1 cut(s)	896
SmlI	CTYRAG	1 cut(s)	482
SmoI	CTYRAG	1 cut(s)	482
SpeI	ACTAGT	1 cut(s)	332
SsiI	CCGC	1 cut(s)	68
SspMI	CTAG	7 cut(s)	5, 107, 333, 477, 614, 618, 902
StyD4I	CCNGG	6 cut(s)	397, 604, 605, 676, 719, 1077
TaaI	ACNGT	7 cut(s)	56, 178, 499, 649, 898, 1060, 1175
TaqI	TCGA	4 cut(s)	102, 193, 415, 825
TaqII	GACCGA	2 cut(s)	32, 705
TatI	WGTACW	1 cut(s)	1190
TfiI	GAWTC	4 cut(s)	306, 435, 576, 1120
Tru1I	TTAA	1 cut(s)	1049
Tru9I	TTAA	1 cut(s)	1049
TscAI	CASTG	2 cut(s)	586, 1065
TseFI	GTSAC	2 cut(s)	178, 1033
Tsp45I	GTSAC	2 cut(s)	178, 1033
TspDTI	ATGAA	4 cut(s)	305, 573, 1019, 1054
TspMI	CCCGGG	1 cut(s)	605
TspRI	CASTG	2 cut(s)	586, 1065
Van91I	CCANNNNNTGG	1 cut(s)	1103
VpaK11BI	GGWCC	1 cut(s)	717
XapI	RAATTY	5 cut(s)	346, 359, 402, 926, 1100
XmaI	CCCGGG	1 cut(s)	605
XmnI	GAANNNNTTC	3 cut(s)	359, 439, 1010
XspI	CTAG	7 cut(s)	5, 107, 333, 477, 614, 618, 902
Zsp2I	ATGCAT	1 cut(s)	138

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.