Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000350

Physical Location & Seq

Genomic Coordinates Forward (+)

43811194 .. 43814581

Gene Structure 3388 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1305700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2739 bp

ATGCAAACGGAAATGTCTGTCTGTCTCACCAGCGCGAATCGCGTCCAAAGGTTCTGGCTTCTGTTTCCCAGGCTCATCATCTACTTTCTCACCATTCTCTCCTATGGAGTTGAAGCTGGCAGTAGAACCAATGTCACCAACGTTGGCGCAGTCATTAACGTTAGTTCTCGTATTGGAAAAGAGCAGAAAACCGCCATGGAGATCGCAGCTGAGAGCTTCAACAACCGTTCAAAGACTCATAAGCTCATCCTTCATTTTCCCGACTCCGGCAGAGACCCCTTTCTTGCTGCTTCTGCTGCTGAAGAGTTGATAAAGGAAAAGAAAGTAGAAGTAATAGTTGGCATGGAGACATGGCAGGAAGCAGCTCAAGTAGCTGATCTTGTCGGAAACCAAGCTCAAATTCCGGTGATTTCGTTTGCAGCACCCTCTATAACCCCGCCACTAATGCAACGCCAATGGCCATCTTTGATACGAATGGCAACAAATGGTTCTGCTCAGATGAAATGCATTGCGGATATAGTTTCAGCCTACCATTGGAAAACGGTGGTTGTGGTATACGAAGATGACGGCTATGGCGGCGATGTTGGGATGCTAGCTCTTTTGACCGAGGCGCTTCAAGATGTCGGTTCAAAGATTGAGCACCGTTTGGTTCTTCCGCAGATTTCTTCGCTGTCTAATCCAAACTGGGTTGAGCTAGAAGAGATGTTGAGGCTTCCCAAAATGCAGTCTCGGGTGTTTATCATTCTCCAGTCATCGTTGCCTACAGTGACTAATCTATTTCGATTGGCTGGGAAGATGGGACTTGTTGGAAAAGAGTCAGCTTGGATCATCACCGAGAGTATTGCAAGTCTGCTTGACCCTCAACACGCCTCTGACATGAAAGGCACCCTGGGAATCAAGACCTACTATGCCAATAATACTAGTTCTTATACAAATTTCCGGAAGAAATTCCAAACGAAGTATTCAGAAGGAAATAGCGCCCAGCCAGGAATTTATGCTCTTCGAGCATATGATGCTATTGGAATCATTTCACAGGCTATAGGAAGAATGACTGATAACACAACTAGCCTTGAGGTGTTGAAAACCGTATTGAGCAGTTATACAGGTTTGAGCGCGAACATGCGGTTAAACAGCGGTGAGGTACTGTACTCTCCAATTTTCAGGATTGTAAATATTTTGGATGAGAATAGATTCCAAGAATTGAACTATTGGACACCGGAACTTGGGTTCTCATCGGATGAAGCAGGGGAAAACAAATTCAGTGATGTTGGTGGAGTAATCTGGCCTGGGAACCTAACTAGTGCCCCGAAAGGCTGGGCAATGCCTACTGTTGAGAAGCCAATGAGAATTGGGGTGCCGGGTAAAACTTCCTTCAGCAAATTTGTGAAGGTTGACTCAGATAAGAAGAAGTCTGTCGACGGTTTCTGCATTGCAATTTTTGATTTGGTCATAAATCGTTTGAACTATTTTCTGCCGTATCAATTCGAAGTTTTCAATGGCTCGTACGATCATCTTGTGGAACAAGTCCATAAAAAGGTTTATGATGCTGTCGTTGGTGACATAACCGTGCTAGCTGACCGGCTGGACAAGGTGGAATTCACTCAGCCATATATGGAGTCTGGTTTGTCAATGATAGTTCCGGCAGAACCCAAAAAATCAACATGGATGTTTATGAAACCTTTCACATGGCAAATGTGGGTGGTGACTGTTTCCATCTTAATCTACACAGTGTTCATCGTATGGTTCCTGGAGCAACCGTCAAATCCAGAATTTGATGGCCCCTTGAAGAATCAGATTGGCACAGCAACTTGGTTCACCTTCTCCTCTTTGTTCTTTGCTCACAAAGAGAAAATCTACAGCAACTTGACGCGAGTGGTGGTCGCAGTTTGGCTCTTTGTTGTACTGATCCTGACCTCAAGCTACACCGCTAATCTCACTTCAATGCTCACCGTTCAGCGACTGAAACCGAATGCAACATACATCGACACCGACTCGAAAGTTGGTTGCGACGGGGACTCATTTGTCATTGAGTACCTGCGGAATGTCCTTGGATTCAAAAAAATCATCGAAGTGAAAAGTGAATACGACTACCCGAAAGAATTCAAAAACAAAACTATAGAGGTCGCCTTTCTTGAACACCCATATGCAGAAGTCTTCATTAATGAGAACTGCATAGGATATACTGCCACTGCACCCACCTATAGATTTGGAGGCTTGAGCTTTATATTCCAGAGAGGTTCTCCAATAGCGCGTGATTTCACAAAGGCGATTCTAGAGCTGTTGGAGAATGGAGATGTGAAGAAGCTACAAAATGAATGGTTGACTCCCAAGGAGTGTCCAAGAAATACAACTTTGAACGAACCAGAAAGCTTGACCCTCAACAGTTTTTCAGTCCTCTATGAAATATCAGCTGCTACTTCCACCTTATGTTTTCTGCTCTCTTTTACTATCCGGCTGAGGAGGTTTCAGCATCAAGAGGCAGCAGAACAAGGCAGTGCAAGTCCGTGCGCGAGCGGAGAAACGCTTTGGAACAAGGCAGTTAGAATGGCAAAATTTTTTTGCAATAGAGAGATTAATGTTCCAACCAGAGCTCCAACTTTAGCACATTACGCAGAAGAATGGAGTTCTCCAAGGTGGGAGCCACTGGCAAAGCTACATAGGAGCAAGGAGGGGCGGCCGCCCCTCCGGTCGTCGGAAATCGCCATGAAAGCAAGAATTCGGCCCCTCCGATCTTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

913

Amino Acids

102.7

Weight (kDa)

8.29

Isoelectric Point (pI)

36.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	57 - 361	1.2e-19	Periplasmic binding protein
ANF_receptor	PF01094	63 - 377	6.9e-41	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	465 - 547	7e-07	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	467 - 775	1.4e-12	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	562 - 804	1.4e-28	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0000350)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g04591 FvH4_1g04600 FvH4_3g08210 FvH4_3g08210 FvH4_3g08211 FvH4_3g32960 FvH4_3g32961 FvH4_5g21700 FvH4_6g22211
malus_domestica	MD05G1305700.v1.1 MD05G1305800.v1.1 MD05G1305900.v1.1 MD05G1306400.v1.1
prunus_persica	Prupe.4G056200_v2.0.a1 Prupe.4G056200_v2.0.a1 Prupe.4G056600_v2.0.a1 Prupe.4G056600_v2.0.a1
pyrus_communis	pycom05g28300 pycom05g28310 pycom05g28350 pycom10g23860
rosa_chinensis	RchiOBHm_Chr5g0009861 RchiOBHm_Chr5g0009881 RchiOBHm_Chr5g0009931 RchiOBHm_Chr5g0009951 RchiOBHm_Chr5g0009981 RchiOBHm_Chr5g0055531 RchiOBHm_Chr5g0055541
rosa_laevigata	RLG00000015779 RLG00000031705 RLG00000031706 RLG00000031711 RLG00000031713 RLG00000031715 RLG00000031716 RLG00000034996
rosa_multiflora	Rmu_co8083594.1_g000001 Rmu_co8206032.1_g000001 Rmu_co8356045.1_g000001 Rmu_sc0000773.1_g000002 Rmu_sc0001709.1_g000001 Rmu_sc0001709.1_g000007 Rmu_sc0001709.1_g000012 Rmu_sc0001709.1_g000013 Rmu_sc0001709.1_g000018 Rmu_sc0001709.1_g000019 Rmu_sc0002275.1_g000004 Rmu_sc0003071.1_g000002 Rmu_sc0003071.1_g000003 Rmu_sc0003630.1_g000014 Rmu_sc0003630.1_g000017 Rmu_sc0003630.1_g000043 Rmu_sc0003630.1_g000044 Rmu_sc0004626.1_g000007
rosa_roxburghii	Rroxscaffold_1G00024770 Rroxscaffold_1G00066180 Rroxscaffold_1G00066200 Rroxscaffold_1G00066210 Rroxscaffold_1G00066240 Rroxscaffold_1G00066250 Rroxscaffold_1G00066320 Rroxscaffold_1G00066340 Rroxscaffold_3G00234120
rosa_rugosa	Rorug04G0446400 Rorug04G0446500 Rorug04G0446600 Rorug04G0447000 Rorug04G0447100 Rorug04G0447200 Rorug04G0447300 Rorug04G0447900 Rorug04G0448200
rosa_samantha	Rh2CG021600 Rh2DG021300 Rh4AG195700 Rh4BG194700 Rh5AG077900 Rh5AG078100 Rh5AG078600 Rh5AG078700 Rh5AG078900 Rh5AG079000 Rh5AG079100 Rh5AG363100 Rh5CG085300 Rh5CG085900 Rh5CG086000 Rh5CG086200 Rh5CG086400 Rh5CG086500 Rh5CG397300 Rh5DG073400 Rh5DG074000 Rh5DG074100 Rh5DG074300 Rh5DG074500 Rh5DG074600 Rh5DG129000 Rh5DG129400 Rh5DG388500
rosa_wichuraiana	Rw2G001690 Rw5G007180 Rw5G007190 Rw5G007230 Rw5G007240 Rw5G007250 Rw5G007320 Rw5G034180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2021
Acc36I	ACCTGC	1 cut(s)	2043
AccB1I	GGYRCC	2 cut(s)	884, 1354
AccBSI	CCGCTC	1 cut(s)	2514
AccI	GTMKAC	2 cut(s)	555, 1416
AccII	CGCG	6 cut(s)	35, 42, 1115, 1870, 2251, 2510
AccIII	TCCGGA	1 cut(s)	939
AclI	AACGTT	2 cut(s)	141, 159
AclWI	GGATC	2 cut(s)	833, 1900
AcoI	YGGCCR	2 cut(s)	458, 2675
AcuI	CTGAAG	2 cut(s)	321, 1357
AfaI	GTAC	5 cut(s)	1143, 1148, 1505, 1902, 2033
AfiI	CCNNNNNNNGG	5 cut(s)	266, 534, 1223, 1534, 2692
AhlI	ACTAGT	2 cut(s)	920, 1298
AjnI	CCWGG	5 cut(s)	68, 888, 985, 1285, 1746
AjuI	GAANNNNNNNTTGG	4 cut(s)	321, 353, 384, 416
AloI	GAACNNNNNNTCC	2 cut(s)	1211, 1243
Alw21I	GWGCWC	2 cut(s)	642, 2593
Alw26I	GTCTC	4 cut(s)	29, 267, 341, 732
AlwI	GGATC	2 cut(s)	833, 1900
AlwNI	CAGNNNCTG	1 cut(s)	1960
Ama87I	CYCGRG	1 cut(s)	729
Aor13HI	TCCGGA	1 cut(s)	939
AoxI	GGCC	5 cut(s)	458, 1283, 1777, 2675, 2720
ApeKI	GCWGC	7 cut(s)	206, 287, 296, 362, 419, 2411, 2480
AseI	ATTAAT	2 cut(s)	2160, 2574
Asp700I	GAANNNNTTC	5 cut(s)	947, 1027, 1190, 1679, 2099
AspLEI	GCGC	7 cut(s)	35, 149, 613, 980, 1115, 2251, 2510
AspS9I	GGNCC	2 cut(s)	1778, 2721
AsuC2I	CCSGG	1 cut(s)	1359
AsuII	TTCGAA	1 cut(s)	1485
AsuNHI	GCTAGC	2 cut(s)	592, 1570
AvaI	CYCGRG	1 cut(s)	729
BaeGI	GKGCMC	1 cut(s)	1306
BalI	TGGCCA	1 cut(s)	460
BanI	GGYRCC	2 cut(s)	884, 1354
BanII	GRGCYC	1 cut(s)	2593
BbsI	GAAGAC	1 cut(s)	2146
Bbv12I	GWGCWC	2 cut(s)	642, 2593
BbvCI	CCTCAGC	1 cut(s)	2456
BbvI	GCAGC	7 cut(s)	218, 274, 283, 374, 431, 2398, 2492
BccI	CCATC	4 cut(s)	469, 790, 1721, 1769
BceAI	ACGGC	2 cut(s)	583, 1459
BcgI	CGANNNNNNTGC	2 cut(s)	824, 858
BciT130I	CCWGG	5 cut(s)	70, 890, 987, 1287, 1748
BcnI	CCSGG	1 cut(s)	1359
BcoDI	GTCTC	4 cut(s)	29, 267, 341, 732
BcuI	ACTAGT	2 cut(s)	920, 1298
BfaI	CTAG	7 cut(s)	593, 695, 921, 1065, 1299, 1571, 2273
BfmI	CTRYAG	5 cut(s)	762, 1038, 1855, 2115, 2200
BfoI	RGCGCY	2 cut(s)	614, 981
BfuAI	ACCTGC	1 cut(s)	2043
Bme1390I	CCNGG	6 cut(s)	70, 890, 987, 1287, 1359, 1748
BmeT110I	CYCGRG	1 cut(s)	729
BmgT120I	GGNCC	2 cut(s)	1778, 2721
BmiI	GGNNCC	7 cut(s)	886, 1292, 1356, 1745, 1780, 2640, 2723
BmrFI	CCNGG	6 cut(s)	70, 890, 987, 1287, 1359, 1748
BmrI	ACTGGG	1 cut(s)	694
BmsI	GCATC	4 cut(s)	579, 1003, 1534, 2479
BmtI	GCTAGC	2 cut(s)	596, 1574
BmuI	ACTGGG	1 cut(s)	694
BpiI	GAAGAC	1 cut(s)	2146
BplI	GAGNNNNNCTC	2 cut(s)	2224, 2256
BpmI	CTGGAG	2 cut(s)	731, 1769
Bpu10I	CCTNAGC	1 cut(s)	2456
Bpu14I	TTCGAA	1 cut(s)	1485
BpuEI	CTTGAG	4 cut(s)	351, 1091, 1900, 2236
BpuMI	CCSGG	1 cut(s)	1359
BsaI	GGTCTC	1 cut(s)	267
BsaJI	CCNNGG	9 cut(s)	68, 195, 606, 888, 889, 1286, 2047, 2328, 2630
BsaWI	WCCGGW	4 cut(s)	403, 939, 1216, 2685
BsaXI	ACNNNNNCTCC	4 cut(s)	1742, 1772, 2575, 2605
Bsc4I	CCNNNNNNNGG	5 cut(s)	266, 534, 1223, 1534, 2692
Bse118I	RCCGGY	1 cut(s)	1578
Bse1I	ACTGG	3 cut(s)	689, 748, 2649
Bse3DI	GCAATG	3 cut(s)	507, 1326, 1428
BseAI	TCCGGA	1 cut(s)	939
BseBI	CCWGG	5 cut(s)	70, 890, 987, 1287, 1748
BseDI	CCNNGG	9 cut(s)	68, 195, 606, 888, 889, 1286, 2047, 2328, 2630
BseGI	GGATG	5 cut(s)	246, 594, 1186, 1243, 1671
BseLI	CCNNNNNNNGG	5 cut(s)	266, 534, 1223, 1534, 2692
BseMI	GCAATG	3 cut(s)	507, 1326, 1428
BseMII	CTCAG	5 cut(s)	201, 509, 1410, 1616, 2447
BseNI	ACTGG	3 cut(s)	689, 748, 2649
BseRI	GAGGAG	2 cut(s)	1813, 2473
BseSI	GKGCMC	1 cut(s)	1306
BseX3I	CGGCCG	1 cut(s)	2675
BseXI	GCAGC	7 cut(s)	218, 274, 283, 374, 431, 2398, 2492
BseYI	CCCAGC	3 cut(s)	788, 981, 1314
BsgI	GTGCAG	1 cut(s)	2175
Bsh1236I	CGCG	6 cut(s)	35, 42, 1115, 1870, 2251, 2510
Bsh1285I	CGRYCG	2 cut(s)	2678, 2690
BshFI	GGCC	5 cut(s)	460, 1285, 1779, 2677, 2722
BshNI	GGYRCC	2 cut(s)	884, 1354
BsiEI	CGRYCG	2 cut(s)	2678, 2690
BsiHKAI	GWGCWC	2 cut(s)	642, 2593
BsiHKCI	CYCGRG	1 cut(s)	729
BsiSI	CCGG	9 cut(s)	267, 404, 940, 1217, 1358, 1579, 1640, 2452, 2686
BsiWI	CGTACG	1 cut(s)	1503
BslFI	GGGAC	2 cut(s)	813, 2027
BslI	CCNNNNNNNGG	5 cut(s)	266, 534, 1223, 1534, 2692
BsmAI	GTCTC	4 cut(s)	29, 267, 341, 732
BsmFI	GGGAC	2 cut(s)	813, 2027
BsmI	GAATGC	1 cut(s)	1975
BsnI	GGCC	5 cut(s)	460, 1285, 1779, 2677, 2722
Bso31I	GGTCTC	1 cut(s)	267
BsoBI	CYCGRG	1 cut(s)	729
Bsp119I	TTCGAA	1 cut(s)	1485
Bsp1286I	GDGCHC	3 cut(s)	642, 1306, 2593
Bsp13I	TCCGGA	1 cut(s)	939
Bsp143I	GATC	6 cut(s)	201, 376, 825, 1507, 1905, 2729
Bsp19I	CCATGG	1 cut(s)	195
BspANI	GGCC	5 cut(s)	460, 1285, 1779, 2677, 2722
BspCNI	CTCAG	5 cut(s)	202, 508, 1409, 1615, 2448
BspEI	TCCGGA	1 cut(s)	939
BspFNI	CGCG	6 cut(s)	35, 42, 1115, 1870, 2251, 2510
BspLI	GGNNCC	7 cut(s)	886, 1292, 1356, 1745, 1780, 2640, 2723
BspMI	ACCTGC	1 cut(s)	2043
BspOI	GCTAGC	2 cut(s)	596, 1574
BspPI	GGATC	2 cut(s)	833, 1900
BspQI	GCTCTTC	1 cut(s)	1005
BspT104I	TTCGAA	1 cut(s)	1485
BspT107I	GGYRCC	2 cut(s)	884, 1354
BspTNI	GGTCTC	1 cut(s)	267
BsrBI	CCGCTC	1 cut(s)	2514
BsrDI	GCAATG	3 cut(s)	507, 1326, 1428
BsrFI	RCCGGY	1 cut(s)	1578
BsrI	ACTGG	3 cut(s)	689, 748, 2649
BssAI	RCCGGY	1 cut(s)	1578
BssECI	CCNNGG	9 cut(s)	68, 195, 606, 888, 889, 1286, 2047, 2328, 2630
BssMI	GATC	6 cut(s)	201, 376, 825, 1507, 1905, 2729
BssNAI	GTATAC	1 cut(s)	556
BssT1I	CCWWGG	4 cut(s)	195, 2047, 2328, 2630
Bst1107I	GTATAC	1 cut(s)	556
Bst2UI	CCWGG	5 cut(s)	70, 890, 987, 1287, 1748
Bst6I	CTCTTC	3 cut(s)	297, 693, 1005
BstAPI	GCANNNNNTGC	1 cut(s)	1013
BstBI	TTCGAA	1 cut(s)	1485
BstC8I	GCNNGC	4 cut(s)	118, 594, 1572, 2512
BstDEI	CTNAG	5 cut(s)	210, 495, 1396, 1602, 2456
BstDSI	CCRYGG	1 cut(s)	195
BstF5I	GGATG	5 cut(s)	246, 594, 1186, 1243, 1671
BstFNI	CGCG	6 cut(s)	35, 42, 1115, 1870, 2251, 2510
BstH2I	RGCGCY	2 cut(s)	614, 981
BstHHI	GCGC	7 cut(s)	35, 149, 613, 980, 1115, 2251, 2510
BstKTI	GATC	6 cut(s)	204, 379, 828, 1510, 1908, 2732
BstMAI	GTCTC	4 cut(s)	29, 267, 341, 732
BstMBI	GATC	6 cut(s)	201, 376, 825, 1507, 1905, 2729
BstMCI	CGRYCG	2 cut(s)	2678, 2690
BstNI	CCWGG	5 cut(s)	70, 890, 987, 1287, 1748
BstNSI	RCATGY	1 cut(s)	1123
BstSCI	CCNGG	6 cut(s)	68, 888, 985, 1285, 1357, 1746
BstSFI	CTRYAG	5 cut(s)	762, 1038, 1855, 2115, 2200
BstSLI	GKGCMC	1 cut(s)	1306
BstUI	CGCG	6 cut(s)	35, 42, 1115, 1870, 2251, 2510
BstV1I	GCAGC	7 cut(s)	218, 274, 283, 374, 431, 2398, 2492
BstV2I	GAAGAC	1 cut(s)	2146
BstZ17I	GTATAC	1 cut(s)	556
BstZI	CGGCCG	1 cut(s)	2675
BsuRI	GGCC	5 cut(s)	460, 1285, 1779, 2677, 2722
BtgI	CCRYGG	1 cut(s)	195
BtgZI	GCGATG	1 cut(s)	594
BtsCI	GGATG	5 cut(s)	246, 594, 1186, 1243, 1671
BtsI	GCAGTG	2 cut(s)	2187, 2500
BtsIMutI	CAGTG	6 cut(s)	771, 1267, 1734, 2187, 2500, 2642
BveI	ACCTGC	1 cut(s)	2043
Cac8I	GCNNGC	4 cut(s)	118, 594, 1572, 2512
CaiI	CAGNNNCTG	1 cut(s)	1960
CciNI	GCGGCCGC	1 cut(s)	2675
CfoI	GCGC	7 cut(s)	35, 149, 613, 980, 1115, 2251, 2510
Cfr10I	RCCGGY	1 cut(s)	1578
Cfr13I	GGNCC	2 cut(s)	1778, 2721
CseI	GACGC	2 cut(s)	31, 1876
Csp6I	GTAC	5 cut(s)	1142, 1147, 1504, 1901, 2032
CviAII	CATG	8 cut(s)	196, 343, 351, 877, 1120, 1662, 1686, 2704
CviQI	GTAC	5 cut(s)	1142, 1147, 1504, 1901, 2032
DdeI	CTNAG	5 cut(s)	210, 495, 1396, 1602, 2456
DpnI	GATC	6 cut(s)	203, 378, 827, 1509, 1907, 2731
DpnII	GATC	6 cut(s)	201, 376, 825, 1507, 1905, 2729
DrdI	GACNNNNNNGTC	1 cut(s)	2021
DseDI	GACNNNNNNGTC	1 cut(s)	2021
EaeI	YGGCCR	2 cut(s)	458, 2675
EagI	CGGCCG	1 cut(s)	2675
Eam1104I	CTCTTC	3 cut(s)	297, 693, 1005
EarI	CTCTTC	3 cut(s)	297, 693, 1005
Ecl136II	GAGCTC	1 cut(s)	2591
EclXI	CGGCCG	1 cut(s)	2675
Eco130I	CCWWGG	4 cut(s)	195, 2047, 2328, 2630
Eco24I	GRGCYC	1 cut(s)	2593
Eco31I	GGTCTC	1 cut(s)	267
Eco52I	CGGCCG	1 cut(s)	2675
Eco53kI	GAGCTC	1 cut(s)	2591
Eco57I	CTGAAG	2 cut(s)	321, 1357
Eco88I	CYCGRG	1 cut(s)	729
EcoICRI	GAGCTC	1 cut(s)	2591
EcoRI	GAATTC	3 cut(s)	1595, 2099, 2715
EcoRII	CCWGG	5 cut(s)	68, 888, 985, 1285, 1746
EcoT14I	CCWWGG	4 cut(s)	195, 2047, 2328, 2630
EcoT22I	ATGCAT	1 cut(s)	509
EcoT38I	GRGCYC	1 cut(s)	2593
ErhI	CCWWGG	4 cut(s)	195, 2047, 2328, 2630
FaeI	CATG	8 cut(s)	199, 346, 354, 880, 1123, 1665, 1689, 2707
FaqI	GGGAC	2 cut(s)	813, 2027
FatI	CATG	8 cut(s)	195, 342, 350, 876, 1119, 1661, 1685, 2703
FauI	CCCGC	1 cut(s)	444
FauNDI	CATATG	2 cut(s)	1009, 2143
FblI	GTMKAC	2 cut(s)	555, 1416
FokI	GGATG	5 cut(s)	233, 601, 1193, 1250, 1678
FriOI	GRGCYC	1 cut(s)	2593
FspBI	CTAG	7 cut(s)	593, 695, 921, 1065, 1299, 1571, 2273
GlaI	GCGC	7 cut(s)	34, 148, 612, 979, 1114, 2250, 2509
GsaI	CCCAGC	3 cut(s)	792, 985, 1318
GsuI	CTGGAG	2 cut(s)	731, 1769
HaeII	RGCGCY	2 cut(s)	614, 981
HaeIII	GGCC	5 cut(s)	460, 1285, 1779, 2677, 2722
HapII	CCGG	9 cut(s)	267, 404, 940, 1217, 1358, 1579, 1640, 2452, 2686
HgaI	GACGC	2 cut(s)	31, 1876
HhaI	GCGC	7 cut(s)	35, 149, 613, 980, 1115, 2251, 2510
Hin1II	CATG	8 cut(s)	199, 346, 354, 880, 1123, 1665, 1689, 2707
Hin6I	GCGC	7 cut(s)	33, 147, 611, 978, 1113, 2249, 2508
HinP1I	GCGC	7 cut(s)	33, 147, 611, 978, 1113, 2249, 2508
HincII	GTYRAC	3 cut(s)	1393, 1417, 2322
HindII	GTYRAC	3 cut(s)	1393, 1417, 2322
HindIII	AAGCTT	1 cut(s)	2368
HpaII	CCGG	9 cut(s)	267, 404, 940, 1217, 1358, 1579, 1640, 2452, 2686
Hpy166II	GTNNAC	5 cut(s)	556, 1393, 1417, 1815, 2322
Hpy8I	GTNNAC	5 cut(s)	556, 1393, 1417, 1815, 2322
Hpy99I	CGWCG	3 cut(s)	1421, 2012, 2695
HpyAV	CCTTC	5 cut(s)	260, 962, 1381, 1381, 1828
HpyCH4IV	ACGT	2 cut(s)	141, 159
HpyF3I	CTNAG	5 cut(s)	210, 495, 1396, 1602, 2456
HpySE526I	ACGT	2 cut(s)	141, 159
Hsp92II	CATG	8 cut(s)	199, 346, 354, 880, 1123, 1665, 1689, 2707
HspAI	GCGC	7 cut(s)	33, 147, 611, 978, 1113, 2249, 2508
Kpn2I	TCCGGA	1 cut(s)	939
Kzo9I	GATC	6 cut(s)	201, 376, 825, 1507, 1905, 2729
LguI	GCTCTTC	1 cut(s)	1005
LmnI	GCTCC	4 cut(s)	1750, 2596, 2638, 2661
Lsp1109I	GCAGC	7 cut(s)	218, 274, 283, 374, 431, 2398, 2492
LweI	GCATC	4 cut(s)	579, 1003, 1534, 2479
MaeI	CTAG	7 cut(s)	593, 695, 921, 1065, 1299, 1571, 2273
MaeII	ACGT	2 cut(s)	141, 159
MaeIII	GTNAC	4 cut(s)	133, 766, 1556, 1702
MalI	GATC	6 cut(s)	203, 378, 827, 1509, 1907, 2731
MbiI	CCGCTC	1 cut(s)	2514
MboI	GATC	6 cut(s)	201, 376, 825, 1507, 1905, 2729
MhlI	GDGCHC	3 cut(s)	642, 1306, 2593
MlsI	TGGCCA	1 cut(s)	460
MluNI	TGGCCA	1 cut(s)	460
MlyI	GAGTC	8 cut(s)	229, 257, 824, 1388, 1625, 1985, 2009, 2317
MmeI	TCCRAC	6 cut(s)	364, 787, 2262, 2606, 2618, 2673
Mox20I	TGGCCA	1 cut(s)	460
Mph1103I	ATGCAT	1 cut(s)	509
MroI	TCCGGA	1 cut(s)	939
MroXI	GAANNNNTTC	5 cut(s)	947, 1027, 1190, 1679, 2099
MscI	TGGCCA	1 cut(s)	460
MseI	TTAA	5 cut(s)	156, 1127, 1718, 2160, 2574
MslI	CAYNNNNRTG	4 cut(s)	1565, 1940, 2069, 2142
Msp20I	TGGCCA	1 cut(s)	460
MspA1I	CMGCKG	3 cut(s)	209, 1134, 2411
MspI	CCGG	9 cut(s)	267, 404, 940, 1217, 1358, 1579, 1640, 2452, 2686
MspR9I	CCNGG	6 cut(s)	70, 890, 987, 1287, 1359, 1748
Mva1269I	GAATGC	1 cut(s)	1975
MvaI	CCWGG	5 cut(s)	70, 890, 987, 1287, 1748
MvnI	CGCG	6 cut(s)	35, 42, 1115, 1870, 2251, 2510
NciI	CCSGG	1 cut(s)	1359
NcoI	CCATGG	1 cut(s)	195
NdeI	CATATG	2 cut(s)	1009, 2143
NdeII	GATC	6 cut(s)	201, 376, 825, 1507, 1905, 2729
NheI	GCTAGC	2 cut(s)	592, 1570
NlaIII	CATG	8 cut(s)	199, 346, 354, 880, 1123, 1665, 1689, 2707
NlaIV	GGNNCC	7 cut(s)	886, 1292, 1356, 1745, 1780, 2640, 2723
NmuCI	GTSAC	4 cut(s)	133, 766, 1556, 1702
NotI	GCGGCCGC	1 cut(s)	2675
NsiI	ATGCAT	1 cut(s)	509
NspI	RCATGY	1 cut(s)	1123
NspV	TTCGAA	1 cut(s)	1485
PasI	CCCWGGG	1 cut(s)	889
PciSI	GCTCTTC	1 cut(s)	1005
PcsI	WCGNNNNNNNCGW	1 cut(s)	2725
PctI	GAATGC	1 cut(s)	1975
PdmI	GAANNNNTTC	5 cut(s)	947, 1027, 1190, 1679, 2099
PfeI	GAWTC	7 cut(s)	37, 894, 1023, 1191, 1789, 2052, 2269
Pfl23II	CGTACG	1 cut(s)	1503
PfoI	TCCNGGA	1 cut(s)	1746
PleI	GAGTC	8 cut(s)	229, 257, 823, 1388, 1624, 1985, 2009, 2317
PpsI	GAGTC	8 cut(s)	229, 257, 823, 1388, 1624, 1985, 2009, 2317
PshBI	ATTAAT	2 cut(s)	2160, 2574
Psp124BI	GAGCTC	1 cut(s)	2593
Psp1406I	AACGTT	2 cut(s)	141, 159
Psp6I	CCWGG	5 cut(s)	68, 888, 985, 1285, 1746
PspFI	CCCAGC	3 cut(s)	788, 981, 1314
PspGI	CCWGG	5 cut(s)	68, 888, 985, 1285, 1746
PspLI	CGTACG	1 cut(s)	1503
PspN4I	GGNNCC	7 cut(s)	886, 1292, 1356, 1745, 1780, 2640, 2723
PspPI	GGNCC	2 cut(s)	1778, 2721
PstNI	CAGNNNCTG	1 cut(s)	1960
PvuII	CAGCTG	2 cut(s)	209, 2411
RsaI	GTAC	5 cut(s)	1143, 1148, 1505, 1902, 2033
RsaNI	GTAC	5 cut(s)	1142, 1147, 1504, 1901, 2032
RseI	CAYNNNNRTG	4 cut(s)	1565, 1940, 2069, 2142
SacI	GAGCTC	1 cut(s)	2593
SalI	GTCGAC	1 cut(s)	1415
SapI	GCTCTTC	1 cut(s)	1005
SaqAI	TTAA	5 cut(s)	156, 1127, 1718, 2160, 2574
Sau3AI	GATC	6 cut(s)	201, 376, 825, 1507, 1905, 2729
Sau96I	GGNCC	2 cut(s)	1778, 2721
SchI	GAGTC	8 cut(s)	229, 257, 824, 1388, 1625, 1985, 2009, 2317
ScrFI	CCNGG	6 cut(s)	70, 890, 987, 1287, 1359, 1748
SduI	GDGCHC	3 cut(s)	642, 1306, 2593
SfaNI	GCATC	4 cut(s)	579, 1003, 1534, 2479
SfcI	CTRYAG	5 cut(s)	762, 1038, 1855, 2115, 2200
SfuI	TTCGAA	1 cut(s)	1485
SmiMI	CAYNNNNRTG	4 cut(s)	1565, 1940, 2069, 2142
SmlI	CTYRAG	4 cut(s)	366, 1070, 1915, 2215
SmoI	CTYRAG	4 cut(s)	366, 1070, 1915, 2215
SpeI	ACTAGT	2 cut(s)	920, 1298
SspI	AATATT	1 cut(s)	1174
SspMI	CTAG	7 cut(s)	593, 695, 921, 1065, 1299, 1571, 2273
SstI	GAGCTC	1 cut(s)	2593
StyD4I	CCNGG	6 cut(s)	68, 888, 985, 1285, 1357, 1746
StyI	CCWWGG	4 cut(s)	195, 2047, 2328, 2630
TaiI	ACGT	2 cut(s)	144, 162
TaqI	TCGA	7 cut(s)	781, 1003, 1416, 1485, 1983, 1994, 2067
TaqII	GACCGA	1 cut(s)	620
TatI	WGTACW	2 cut(s)	1146, 1900
TauI	GCSGC	3 cut(s)	579, 2677, 2680
TfiI	GAWTC	7 cut(s)	37, 894, 1023, 1191, 1789, 2052, 2269
Tru1I	TTAA	5 cut(s)	156, 1127, 1718, 2160, 2574
Tru9I	TTAA	5 cut(s)	156, 1127, 1718, 2160, 2574
TscAI	CASTG	6 cut(s)	771, 1267, 1734, 2194, 2500, 2649
TseFI	GTSAC	4 cut(s)	133, 766, 1556, 1702
TseI	GCWGC	7 cut(s)	206, 287, 296, 362, 419, 2411, 2480
Tsp45I	GTSAC	4 cut(s)	133, 766, 1556, 1702
TspGWI	ACGGA	2 cut(s)	23, 2493
TspRI	CASTG	6 cut(s)	771, 1267, 1734, 2194, 2500, 2649
VspI	ATTAAT	2 cut(s)	2160, 2574
XbaI	TCTAGA	1 cut(s)	2272
XceI	RCATGY	1 cut(s)	1123
XmiI	GTMKAC	2 cut(s)	555, 1416
XmnI	GAANNNNTTC	5 cut(s)	947, 1027, 1190, 1679, 2099
XspI	CTAG	7 cut(s)	593, 695, 921, 1065, 1299, 1571, 2273
Zsp2I	ATGCAT	1 cut(s)	509

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.