Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0000350

Physical Location & Seq

Genomic Coordinates Reverse (-)

25837928 .. 25839936

Gene Structure 2009 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g23860.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1521 bp

ATGTGGGGAAGGTTAGATCAGAACAAAATGTCTTTTCCTTTCCCTAAACTTGGGCTCTTGCTTCCTGTGCTCATCGTCTGCTTGCTAATTGCTATCTCCCATGGAGTTGGAGCTAACAATAGTAGCAATGTCACCAACATTGGAGCAATCATTAATGCTAATGGCCGTATTGGGAAAGAACAGAAAACTGCCATGGAAATTGCAGCTGAAAACTTCAACAACTGTTCGAAGACTCATAAGCTAGTCCTTCATTTTCGAGACTCTGGCAGAGACCCCTTTCGTGCTTCTTCTGCTGTAGCTGATCGTGCAGGAAACCAATCAAATGTTTCGGTGATTTCATTTGCATCAACAGCTATCACCCAGCCCCTAATCCAAAGCCGCTGGCCATATCTGATACGAATGGCAACAGACGGTTCTGCTCAGTTGAAATGCATAGCAGATATGGTTTCTGCGTACAATTGGAAAAGGGTTGTTGTAGTCTACGAAGATGATGGTCACGGTGGAGCTGTTGGGATGCTAGCTGTTTTATCTGAGGCTCTTCGAGATGTTGGTTCGAAGATTGAGCACCATTTGATTCTCCCGCGAGTTTCTTCAATGTCTAATCCAAATTGGGATGGATTAGAAGAGATATTGAGCCTTCCCACCATACAATCCCGTGTGTTTATTGTTATTCAGTCATCTTTTCCTACAGTGACTCATCTGTTTTGTGTAGCGAAGAAGATGGGACTTGTAGGAAGAGACTCAGCTTGGATAATCACAGAGAGTGTCATTAGTGATTTTCCTGACTCTCTTGACAACTCTGATATGGAAGGCACTCTCGGAAGCAAGACCTACTATGCTAATAATTCTAGTTCTTGTACGAAGTTCCTAAAGGAACTCGAAACAAAGTTTTCAGACGAAGATAACACTGCGCCAATGCCAGGAATTTATGCCCTTCAAGCCTACGATATGATGACCGTCATTGCACAGGCCATAACAATAATGACTAGTAACAACAGTGCTACTACTAGTAGTAGCCTGCAGGTGTTGTTAAACATATTGTTGAATAATTGTACTGGTCTGAGCGGTAAAATGTACTTCAAAGAAGGTGAGGTACGTTTTTATTCTCCACGATTTAGGATCATAAATGTCGTTGATGGGAAGATACATAGAGATGTAGGGTTTTCAGAAAGCGTTGTCAGGCAAGGCACAAACAGACGTAATGATGTTGGTATAATTTGGCCCGGGAAGGCCAAAAGTGCCCCAAAAGAGAGAACGTATGCTTTCCATGTTCATCGAAATATTGACCAACTGAAGGATTATGATGCTGTGATTGTTGATGTGACTGTGCTACCTGATCAATTGGACAGAGTGGACTTCACTCAGCCATATATCGAGTCTGGTTTGTCGATGATTGTTCCCGAAAAATATGAGGAGTCGACATGGCTATGTCTGAAGCCTTTCACTTGGCAAGTGTGGGTGGCGTCTGGTGGCCTATTACTCTACACAACACCAATAGTCTGGTTCCTCGAGGGCCCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

507

Amino Acids

56.02

Weight (kDa)

6.38

Isoelectric Point (pI)

41.57

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ANF_receptor	PF01094	95 - 378	8.2e-33	Receptor family ligand binding region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000350)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g04591 FvH4_1g04600 FvH4_3g08210 FvH4_3g08210 FvH4_3g08211 FvH4_3g32960 FvH4_3g32961 FvH4_5g21700 FvH4_6g22211
malus_domestica	MD05G1305700.v1.1 MD05G1305800.v1.1 MD05G1305900.v1.1 MD05G1306400.v1.1
prunus_persica	Prupe.4G056200_v2.0.a1 Prupe.4G056200_v2.0.a1 Prupe.4G056600_v2.0.a1 Prupe.4G056600_v2.0.a1
pyrus_communis	pycom05g28300 pycom05g28310 pycom05g28350 pycom10g23860
rosa_chinensis	RchiOBHm_Chr5g0009861 RchiOBHm_Chr5g0009881 RchiOBHm_Chr5g0009931 RchiOBHm_Chr5g0009951 RchiOBHm_Chr5g0009981 RchiOBHm_Chr5g0055531 RchiOBHm_Chr5g0055541
rosa_laevigata	RLG00000015779 RLG00000031705 RLG00000031706 RLG00000031711 RLG00000031713 RLG00000031715 RLG00000031716 RLG00000034996
rosa_multiflora	Rmu_co8083594.1_g000001 Rmu_co8206032.1_g000001 Rmu_co8356045.1_g000001 Rmu_sc0000773.1_g000002 Rmu_sc0001709.1_g000001 Rmu_sc0001709.1_g000007 Rmu_sc0001709.1_g000012 Rmu_sc0001709.1_g000013 Rmu_sc0001709.1_g000018 Rmu_sc0001709.1_g000019 Rmu_sc0002275.1_g000004 Rmu_sc0003071.1_g000002 Rmu_sc0003071.1_g000003 Rmu_sc0003630.1_g000014 Rmu_sc0003630.1_g000017 Rmu_sc0003630.1_g000043 Rmu_sc0003630.1_g000044 Rmu_sc0004626.1_g000007
rosa_roxburghii	Rroxscaffold_1G00024770 Rroxscaffold_1G00066180 Rroxscaffold_1G00066200 Rroxscaffold_1G00066210 Rroxscaffold_1G00066240 Rroxscaffold_1G00066250 Rroxscaffold_1G00066320 Rroxscaffold_1G00066340 Rroxscaffold_3G00234120
rosa_rugosa	Rorug04G0446400 Rorug04G0446500 Rorug04G0446600 Rorug04G0447000 Rorug04G0447100 Rorug04G0447200 Rorug04G0447300 Rorug04G0447900 Rorug04G0448200
rosa_samantha	Rh2CG021600 Rh2DG021300 Rh4AG195700 Rh4BG194700 Rh5AG077900 Rh5AG078100 Rh5AG078600 Rh5AG078700 Rh5AG078900 Rh5AG079000 Rh5AG079100 Rh5AG363100 Rh5CG085300 Rh5CG085900 Rh5CG086000 Rh5CG086200 Rh5CG086400 Rh5CG086500 Rh5CG397300 Rh5DG073400 Rh5DG074000 Rh5DG074100 Rh5DG074300 Rh5DG074500 Rh5DG074600 Rh5DG129000 Rh5DG129400 Rh5DG388500
rosa_wichuraiana	Rw2G001690 Rw5G007180 Rw5G007190 Rw5G007230 Rw5G007240 Rw5G007250 Rw5G007320 Rw5G034180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1012
AbsI	CCTCGAGG	1 cut(s)	1508
Acc36I	ACCTGC	1 cut(s)	1012
AccBSI	CCGCTC	1 cut(s)	1065
AccI	GTMKAC	2 cut(s)	480, 1418
AccII	CGCG	1 cut(s)	583
AciI	CCGC	3 cut(s)	379, 581, 1065
AclWI	GGATC	1 cut(s)	1127
AcoI	YGGCCR	2 cut(s)	163, 383
AcsI	RAATTY	1 cut(s)	924
AcuI	CTGAAG	2 cut(s)	1313, 1454
AcyI	GRCGYC	1 cut(s)	1463
AfaI	GTAC	5 cut(s)	455, 859, 1054, 1076, 1095
AfiI	CCNNNNNNNGG	3 cut(s)	50, 920, 1294
AgsI	TTSAA	6 cut(s)	217, 427, 594, 938, 1045, 1081
AhlI	ACTAGT	2 cut(s)	986, 1007
AjnI	CCWGG	1 cut(s)	919
AluBI	AGCT	8 cut(s)	113, 206, 241, 299, 353, 506, 521, 746
AluI	AGCT	8 cut(s)	113, 206, 241, 299, 353, 506, 521, 746
Alw21I	GWGCWC	2 cut(s)	72, 567
Alw26I	GTCTC	3 cut(s)	252, 264, 732
AlwI	GGATC	1 cut(s)	1127
Ama87I	CYCGRG	2 cut(s)	1223, 1508
AoxI	GGCC	7 cut(s)	163, 383, 969, 1220, 1230, 1471, 1513
ApaI	GGGCCC	1 cut(s)	1517
ApeKI	GCWGC	1 cut(s)	203
ApoI	RAATTY	1 cut(s)	924
AseI	ATTAAT	1 cut(s)	153
Asp700I	GAANNNNTTC	1 cut(s)	1439
AspLEI	GCGC	1 cut(s)	913
AspS9I	GGNCC	3 cut(s)	1221, 1513, 1514
AsuC2I	CCSGG	2 cut(s)	1224, 1225
AsuHPI	GGTGA	4 cut(s)	124, 343, 349, 1100
AsuII	TTCGAA	2 cut(s)	227, 554
AsuNHI	GCTAGC	1 cut(s)	517
AvaI	CYCGRG	2 cut(s)	1223, 1508
BaeGI	GKGCMC	2 cut(s)	1243, 1517
BalI	TGGCCA	1 cut(s)	385
BanII	GRGCYC	2 cut(s)	57, 1517
BarI	GAAGNNNNNNTAC	2 cut(s)	1077, 1109
BbsI	GAAGAC	1 cut(s)	236
Bbv12I	GWGCWC	2 cut(s)	72, 567
BbvI	GCAGC	1 cut(s)	215
BccI	CCATC	4 cut(s)	485, 608, 715, 1130
BceAI	ACGGC	1 cut(s)	150
BciT130I	CCWGG	1 cut(s)	921
BclI	TGATCA	1 cut(s)	1336
BcnI	CCSGG	2 cut(s)	1224, 1225
BcoDI	GTCTC	3 cut(s)	252, 264, 732
BcuI	ACTAGT	2 cut(s)	986, 1007
BfaI	CTAG	5 cut(s)	242, 518, 849, 987, 1008
BfmI	CTRYAG	3 cut(s)	294, 687, 1019
BfuAI	ACCTGC	1 cut(s)	1012
BisI	GCNGC	2 cut(s)	204, 379
BlsI	GCNGC	2 cut(s)	205, 380
Bme1390I	CCNGG	3 cut(s)	921, 1224, 1225
BmeT110I	CYCGRG	2 cut(s)	1223, 1508
BmgT120I	GGNCC	3 cut(s)	1221, 1513, 1514
BmiI	GGNNCC	2 cut(s)	1505, 1515
BmrFI	CCNGG	3 cut(s)	921, 1224, 1225
BmsI	GCATC	3 cut(s)	353, 504, 1294
BmtI	GCTAGC	1 cut(s)	521
BpiI	GAAGAC	1 cut(s)	236
Bpu14I	TTCGAA	2 cut(s)	227, 554
BpuMI	CCSGG	2 cut(s)	1224, 1225
BsaHI	GRCGYC	1 cut(s)	1463
BsaI	GGTCTC	1 cut(s)	264
BsaJI	CCNNGG	3 cut(s)	100, 192, 1223
Bsc4I	CCNNNNNNNGG	3 cut(s)	50, 920, 1294
Bse1I	ACTGG	1 cut(s)	1060
Bse3DI	GCAATG	2 cut(s)	133, 960
BseBI	CCWGG	1 cut(s)	921
BseDI	CCNNGG	3 cut(s)	100, 192, 1223
BseGI	GGATG	2 cut(s)	519, 619
BseLI	CCNNNNNNNGG	3 cut(s)	50, 920, 1294
BseMI	GCAATG	2 cut(s)	133, 960
BseMII	CTCAG	5 cut(s)	434, 522, 756, 1052, 1376
BseNI	ACTGG	1 cut(s)	1060
BseRI	GAGGAG	1 cut(s)	1427
BseSI	GKGCMC	2 cut(s)	1243, 1517
BseXI	GCAGC	1 cut(s)	215
BseYI	CCCAGC	1 cut(s)	360
BsgI	GTGCAG	1 cut(s)	327
Bsh1236I	CGCG	1 cut(s)	583
BshFI	GGCC	7 cut(s)	165, 385, 971, 1222, 1232, 1473, 1515
BsiHKAI	GWGCWC	2 cut(s)	72, 567
BsiHKCI	CYCGRG	2 cut(s)	1223, 1508
BsiSI	CCGG	1 cut(s)	1224
BslFI	GGGAC	1 cut(s)	738
BslI	CCNNNNNNNGG	3 cut(s)	50, 920, 1294
BsmAI	GTCTC	3 cut(s)	252, 264, 732
BsmFI	GGGAC	1 cut(s)	738
BsnI	GGCC	7 cut(s)	165, 385, 971, 1222, 1232, 1473, 1515
Bso31I	GGTCTC	1 cut(s)	264
BsoBI	CYCGRG	2 cut(s)	1223, 1508
Bsp119I	TTCGAA	2 cut(s)	227, 554
Bsp120I	GGGCCC	1 cut(s)	1513
Bsp1286I	GDGCHC	5 cut(s)	57, 72, 567, 1243, 1517
Bsp143I	GATC	4 cut(s)	16, 301, 1119, 1336
Bsp19I	CCATGG	2 cut(s)	100, 192
BspACI	CCGC	3 cut(s)	379, 581, 1065
BspANI	GGCC	7 cut(s)	165, 385, 971, 1222, 1232, 1473, 1515
BspCNI	CTCAG	5 cut(s)	433, 523, 755, 1053, 1375
BspFNI	CGCG	1 cut(s)	583
BspLI	GGNNCC	2 cut(s)	1505, 1515
BspMAI	CTGCAG	1 cut(s)	1023
BspMI	ACCTGC	1 cut(s)	1012
BspOI	GCTAGC	1 cut(s)	521
BspPI	GGATC	1 cut(s)	1127
BspQI	GCTCTTC	1 cut(s)	543
BspT104I	TTCGAA	2 cut(s)	227, 554
BspTNI	GGTCTC	1 cut(s)	264
BsrBI	CCGCTC	1 cut(s)	1065
BsrDI	GCAATG	2 cut(s)	133, 960
BsrI	ACTGG	1 cut(s)	1060
BssECI	CCNNGG	3 cut(s)	100, 192, 1223
BssMI	GATC	4 cut(s)	16, 301, 1119, 1336
BssNI	GRCGYC	1 cut(s)	1463
BssT1I	CCWWGG	2 cut(s)	100, 192
Bst2UI	CCWGG	1 cut(s)	921
Bst4CI	ACNGT	7 cut(s)	224, 413, 500, 691, 958, 998, 1327
Bst6I	CTCTTC	3 cut(s)	543, 618, 730
BstACI	GRCGYC	1 cut(s)	1463
BstBI	TTCGAA	2 cut(s)	227, 554
BstC8I	GCNNGC	4 cut(s)	83, 383, 519, 1019
BstDEI	CTNAG	5 cut(s)	420, 531, 742, 1061, 1362
BstDSI	CCRYGG	2 cut(s)	100, 192
BstF5I	GGATG	2 cut(s)	519, 619
BstFNI	CGCG	1 cut(s)	583
BstHHI	GCGC	1 cut(s)	913
BstKTI	GATC	4 cut(s)	19, 304, 1122, 1339
BstMAI	GTCTC	3 cut(s)	252, 264, 732
BstMBI	GATC	4 cut(s)	16, 301, 1119, 1336
BstMWI	GCNNNNNNNGC	6 cut(s)	67, 290, 305, 350, 938, 1238
BstNI	CCWGG	1 cut(s)	921
BstSCI	CCNGG	3 cut(s)	919, 1222, 1223
BstSFI	CTRYAG	3 cut(s)	294, 687, 1019
BstSLI	GKGCMC	2 cut(s)	1243, 1517
BstUI	CGCG	1 cut(s)	583
BstV1I	GCAGC	1 cut(s)	215
BstV2I	GAAGAC	1 cut(s)	236
BstXI	CCANNNNNNTGG	2 cut(s)	107, 1500
BsuRI	GGCC	7 cut(s)	165, 385, 971, 1222, 1232, 1473, 1515
BtgI	CCRYGG	2 cut(s)	100, 192
BtsCI	GGATG	2 cut(s)	519, 619
BtsI	GCAGTG	1 cut(s)	906
BtsIMutI	CAGTG	3 cut(s)	696, 906, 1003
BveI	ACCTGC	1 cut(s)	1012
Cac8I	GCNNGC	4 cut(s)	83, 383, 519, 1019
CfoI	GCGC	1 cut(s)	913
Cfr13I	GGNCC	3 cut(s)	1221, 1513, 1514
Cfr9I	CCCGGG	1 cut(s)	1223
CseI	GACGC	1 cut(s)	1452
Csp6I	GTAC	5 cut(s)	454, 858, 1053, 1075, 1094
CviAII	CATG	4 cut(s)	101, 193, 1268, 1422
CviQI	GTAC	5 cut(s)	454, 858, 1053, 1075, 1094
DdeI	CTNAG	5 cut(s)	420, 531, 742, 1061, 1362
DpnI	GATC	4 cut(s)	18, 303, 1121, 1338
DpnII	GATC	4 cut(s)	16, 301, 1119, 1336
EaeI	YGGCCR	2 cut(s)	163, 383
Eam1104I	CTCTTC	3 cut(s)	543, 618, 730
EarI	CTCTTC	3 cut(s)	543, 618, 730
Eco130I	CCWWGG	2 cut(s)	100, 192
Eco24I	GRGCYC	2 cut(s)	57, 1517
Eco31I	GGTCTC	1 cut(s)	264
Eco57I	CTGAAG	2 cut(s)	1313, 1454
Eco88I	CYCGRG	2 cut(s)	1223, 1508
EcoO109I	RGGNCCY	1 cut(s)	1513
EcoRII	CCWGG	1 cut(s)	919
EcoT14I	CCWWGG	2 cut(s)	100, 192
EcoT22I	ATGCAT	1 cut(s)	434
EcoT38I	GRGCYC	2 cut(s)	57, 1517
ErhI	CCWWGG	2 cut(s)	100, 192
FaeI	CATG	4 cut(s)	104, 196, 1271, 1425
FaqI	GGGAC	1 cut(s)	738
FatI	CATG	4 cut(s)	100, 192, 1267, 1421
FauI	CCCGC	1 cut(s)	588
FbaI	TGATCA	1 cut(s)	1336
FblI	GTMKAC	2 cut(s)	480, 1418
Fnu4HI	GCNGC	2 cut(s)	204, 379
FokI	GGATG	2 cut(s)	526, 626
FriOI	GRGCYC	2 cut(s)	57, 1517
Fsp4HI	GCNGC	2 cut(s)	204, 379
FspBI	CTAG	5 cut(s)	242, 518, 849, 987, 1008
GlaI	GCGC	1 cut(s)	912
GluI	GCNGC	2 cut(s)	204, 379
GsaI	CCCAGC	1 cut(s)	364
HaeIII	GGCC	7 cut(s)	165, 385, 971, 1222, 1232, 1473, 1515
HapII	CCGG	1 cut(s)	1224
HgaI	GACGC	1 cut(s)	1452
HhaI	GCGC	1 cut(s)	913
Hin1I	GRCGYC	1 cut(s)	1463
Hin1II	CATG	4 cut(s)	104, 196, 1271, 1425
Hin6I	GCGC	1 cut(s)	911
HinP1I	GCGC	1 cut(s)	911
HincII	GTYRAC	1 cut(s)	1419
HindII	GTYRAC	1 cut(s)	1419
HinfI	GANTC	8 cut(s)	232, 260, 574, 694, 740, 785, 1376, 1415
HpaII	CCGG	1 cut(s)	1224
HphI	GGTGA	4 cut(s)	124, 343, 349, 1100
Hpy166II	GTNNAC	3 cut(s)	481, 1354, 1419
Hpy188I	TCNGA	9 cut(s)	21, 393, 532, 802, 821, 895, 1062, 1168, 1434
Hpy188III	TCNNGA	5 cut(s)	257, 542, 782, 791, 1400
Hpy8I	GTNNAC	3 cut(s)	481, 1354, 1419
HpyAV	CCTTC	8 cut(s)	3, 257, 647, 803, 944, 1079, 1222, 1288
HpyCH4III	ACNGT	7 cut(s)	224, 413, 500, 691, 958, 998, 1327
HpyCH4IV	ACGT	3 cut(s)	1096, 1198, 1256
HpyCH4V	TGCA	6 cut(s)	203, 308, 344, 432, 965, 1021
HpyF10VI	GCNNNNNNNGC	6 cut(s)	67, 290, 305, 350, 938, 1238
HpyF3I	CTNAG	5 cut(s)	420, 531, 742, 1061, 1362
HpySE526I	ACGT	3 cut(s)	1096, 1198, 1256
Hsp92I	GRCGYC	1 cut(s)	1463
Hsp92II	CATG	4 cut(s)	104, 196, 1271, 1425
HspAI	GCGC	1 cut(s)	911
Ksp22I	TGATCA	1 cut(s)	1336
Kzo9I	GATC	4 cut(s)	16, 301, 1119, 1336
LguI	GCTCTTC	1 cut(s)	543
LmnI	GCTCC	3 cut(s)	110, 143, 503
Lsp1109I	GCAGC	1 cut(s)	215
LweI	GCATC	3 cut(s)	353, 504, 1294
MaeI	CTAG	5 cut(s)	242, 518, 849, 987, 1008
MaeII	ACGT	3 cut(s)	1096, 1198, 1256
MaeIII	GTNAC	5 cut(s)	130, 494, 691, 989, 1321
MalI	GATC	4 cut(s)	18, 303, 1121, 1338
MbiI	CCGCTC	1 cut(s)	1065
MboI	GATC	4 cut(s)	16, 301, 1119, 1336
MfeI	CAATTG	2 cut(s)	457, 1340
MhlI	GDGCHC	5 cut(s)	57, 72, 567, 1243, 1517
MlsI	TGGCCA	1 cut(s)	385
MluCI	AATT	9 cut(s)	87, 198, 457, 607, 844, 924, 1048, 1215, 1340
MluNI	TGGCCA	1 cut(s)	385
MlyI	GAGTC	7 cut(s)	226, 254, 688, 734, 779, 1385, 1424
MmeI	TCCRAC	1 cut(s)	88
MnlI	CCTC	5 cut(s)	526, 1084, 1405, 1504, 1517
Mox20I	TGGCCA	1 cut(s)	385
Mph1103I	ATGCAT	1 cut(s)	434
MroXI	GAANNNNTTC	1 cut(s)	1439
MscI	TGGCCA	1 cut(s)	385
MseI	TTAA	2 cut(s)	153, 1031
MslI	CAYNNNNRTG	2 cut(s)	1152, 1426
Msp20I	TGGCCA	1 cut(s)	385
MspA1I	CMGCKG	2 cut(s)	206, 381
MspI	CCGG	1 cut(s)	1224
MspR9I	CCNGG	3 cut(s)	921, 1224, 1225
MunI	CAATTG	2 cut(s)	457, 1340
MvaI	CCWGG	1 cut(s)	921
MvnI	CGCG	1 cut(s)	583
MwoI	GCNNNNNNNGC	6 cut(s)	67, 290, 305, 350, 938, 1238
NciI	CCSGG	2 cut(s)	1224, 1225
NcoI	CCATGG	2 cut(s)	100, 192
NdeII	GATC	4 cut(s)	16, 301, 1119, 1336
NheI	GCTAGC	1 cut(s)	517
NlaIII	CATG	4 cut(s)	104, 196, 1271, 1425
NlaIV	GGNNCC	2 cut(s)	1505, 1515
NmuCI	GTSAC	4 cut(s)	130, 494, 691, 1321
NsiI	ATGCAT	1 cut(s)	434
NspV	TTCGAA	2 cut(s)	227, 554
PaeR7I	CTCGAG	1 cut(s)	1508
PaqCI	CACCTGC	1 cut(s)	1012
PciSI	GCTCTTC	1 cut(s)	543
PdmI	GAANNNNTTC	1 cut(s)	1439
PfeI	GAWTC	1 cut(s)	574
PkrI	GCNGC	2 cut(s)	205, 380
PleI	GAGTC	7 cut(s)	226, 254, 688, 734, 779, 1384, 1423
PpsI	GAGTC	7 cut(s)	226, 254, 688, 734, 779, 1384, 1423
PshBI	ATTAAT	1 cut(s)	153
Psp6I	CCWGG	1 cut(s)	919
PspFI	CCCAGC	1 cut(s)	360
PspGI	CCWGG	1 cut(s)	919
PspN4I	GGNNCC	2 cut(s)	1505, 1515
PspOMI	GGGCCC	1 cut(s)	1513
PspPI	GGNCC	3 cut(s)	1221, 1513, 1514
PspXI	VCTCGAGB	1 cut(s)	1508
PstI	CTGCAG	1 cut(s)	1023
PvuII	CAGCTG	1 cut(s)	206
RsaI	GTAC	5 cut(s)	455, 859, 1054, 1076, 1095
RsaNI	GTAC	5 cut(s)	454, 858, 1053, 1075, 1094
RseI	CAYNNNNRTG	2 cut(s)	1152, 1426
SalI	GTCGAC	1 cut(s)	1417
SapI	GCTCTTC	1 cut(s)	543
SaqAI	TTAA	2 cut(s)	153, 1031
SatI	GCNGC	2 cut(s)	204, 379
Sau3AI	GATC	4 cut(s)	16, 301, 1119, 1336
Sau96I	GGNCC	3 cut(s)	1221, 1513, 1514
SbfI	CCTGCAGG	1 cut(s)	1023
SchI	GAGTC	7 cut(s)	226, 254, 688, 734, 779, 1385, 1424
ScrFI	CCNGG	3 cut(s)	921, 1224, 1225
SdaI	CCTGCAGG	1 cut(s)	1023
SduI	GDGCHC	5 cut(s)	57, 72, 567, 1243, 1517
SfaNI	GCATC	3 cut(s)	353, 504, 1294
SfcI	CTRYAG	3 cut(s)	294, 687, 1019
Sfr274I	CTCGAG	1 cut(s)	1508
SfuI	TTCGAA	2 cut(s)	227, 554
SlaI	CTCGAG	1 cut(s)	1508
SmaI	CCCGGG	1 cut(s)	1225
SmiMI	CAYNNNNRTG	2 cut(s)	1152, 1426
SmlI	CTYRAG	1 cut(s)	1508
SmoI	CTYRAG	1 cut(s)	1508
SpeI	ACTAGT	2 cut(s)	986, 1007
Sse8387I	CCTGCAGG	1 cut(s)	1023
Sse9I	AATT	9 cut(s)	87, 198, 457, 607, 844, 924, 1048, 1215, 1340
SsiI	CCGC	3 cut(s)	379, 581, 1065
SspI	AATATT	1 cut(s)	1282
SspMI	CTAG	5 cut(s)	242, 518, 849, 987, 1008
StyD4I	CCNGG	3 cut(s)	919, 1222, 1223
StyI	CCWWGG	2 cut(s)	100, 192
TaaI	ACNGT	7 cut(s)	224, 413, 500, 691, 958, 998, 1327
TaiI	ACGT	3 cut(s)	1099, 1201, 1259
TasI	AATT	9 cut(s)	87, 198, 457, 607, 844, 924, 1048, 1215, 1340
TatI	WGTACW	2 cut(s)	1052, 1074
TauI	GCSGC	1 cut(s)	381
TfiI	GAWTC	1 cut(s)	574
Tru1I	TTAA	2 cut(s)	153, 1031
Tru9I	TTAA	2 cut(s)	153, 1031
TscAI	CASTG	3 cut(s)	696, 913, 1003
TseFI	GTSAC	4 cut(s)	130, 494, 691, 1321
TseI	GCWGC	1 cut(s)	203
Tsp45I	GTSAC	4 cut(s)	130, 494, 691, 1321
TspDTI	ATGAA	3 cut(s)	239, 327, 1262
TspMI	CCCGGG	1 cut(s)	1223
TspRI	CASTG	3 cut(s)	696, 913, 1003
VspI	ATTAAT	1 cut(s)	153
XapI	RAATTY	1 cut(s)	924
XhoI	CTCGAG	1 cut(s)	1508
XmaI	CCCGGG	1 cut(s)	1223
XmiI	GTMKAC	2 cut(s)	480, 1418
XmnI	GAANNNNTTC	1 cut(s)	1439
XspI	CTAG	5 cut(s)	242, 518, 849, 987, 1008
Zsp2I	ATGCAT	1 cut(s)	434

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.