Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0000350

Physical Location & Seq

Genomic Coordinates Forward (+)

6528009 .. 6531188

Gene Structure 3180 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ29071

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2601 bp

ATGTCTTATTCTACAGCTAAAACAGTCCAAAGACTTGCGATCTTGTTTCCTCTGCTCATCATCTACTTGCTATTCAATCTCTCCTACGCAGTTGAAGCTGAAGATGAGAAAAAAATCACTAACATTGGAGCAATCATTGATGTTAATTCCCGTATCGGGAAAGAACAGAAAGCTGCTATGGAAATTGCAGCTGAAAACTTCAACAACCAGTCAGACACTCATGAGCTAATCCTTCATTTTCGAGATTCTGGCCGCGACCCTTTTCTTGCTGCTTATGCTGCTAAACATTTGAAAAGGGAACAAAAGGTACAAGTGGTAATTGGCATGGAAACTTGGCAGGAAGCAGTTACAGTAGCTGATGTGGTCAAAAACCTGTCTGATCAAATTCCGGTCATTTCCTTTGCAGCACCCACCATATCCCCGCCACTAATACAACGGCGTTGGCCTTCCTTGATACGAATGGCTACCGATGGTGCTGCTCAGATGAAATGCATTGGAGATATAGTTAATGCATACAACTGGAAAAGGGTTGTTGTAATATATGAAGATGATGGTTATGGTGGTGGTGTGGGGAGGCTGGCTATTTTATCTGAGACTCTAAGAAATGTTGGTTCTACAATTGAGCTTCGTATAGTTCTCCCACGACTTTCTGAGCCTGATACAAATTGGGATGAAATAAAGAAGCAGCTGTTGGAGATTTCCAATGTAAACTCTCGGGCATTTATAGTTCTTCAGTCATCTTTACCAACAGTAACTGGTCTATTCAAAGTGGCTAGGAAGATGGGATTTGTAGGAAATGATTCAGCTTGGATAATCACAGAGAGTATTGCTAGTTTGCTTGACCCTCATGGAAACTACGATATGGAAGGAACTCTGGGAATCAAGACCTACTACGCCAATGAGTCTAGTTCTTATGCTGAATTCCAGAAGGAATTCCAAGCTAAGCATGCAGAAGAAAATAACTCCAAGCCAGGAATTTATGCTCTTCGAGCATATGATACTATTAATGCCATTTCACAGGCCATAGAAGGAATCCCTAATAACACCACTAGTACTAGCCTTCAGGGTATGATAACTAGATCGTTGAGCAATTACAACGGTTTAAGTGGGAAAATGCAACTAAAAGGAGGTGAGGTACCGCTGGATTCTCCGAGATTCAGGATCATAAATATTGTGGGTGGGAAGACAGATAACGAATTAAACTTTTGGATACCAGACTTTGGATTTTCAGAAAGCCTTGAAAAAGTATCAGACAAATACAGAAGTAATGATGGTGTTGGCAAAGTCATTTGGCCCGGGAACCCAACACTGCCCTCCAAAGGCTGGGCAATGCCCACTGATGAGAAGCCAATGAAAATTTTGCTCCCAGCTTACCACGAAAATTTTGTAAGGCATATCAGCGGGGAGAACTCAAATGAGATGGGAAAAGAGGGTTTATGTTTAGGAAAGGAAAAACACGGTTTATGCTCAGGAAAGATATATGACGGTTTGAGTATTGATCTTTTCTGTTGCGTGCTATCTAAATTGAATTATTCCTTGCCACATGAATTTGAGTTATTTAACAAGGCCAATGGCACTTACAATGATATGGTAGAACTAGTCTATAACAAGACTTTTGATGCTGTGATTGGCGATATGACTGTACTTGCATCGCGAATTGACAAAGTGGATTTCACTCAACCATATCTGGAGTCTGGTTTGTCATTGATAGTACCAGATAAATCTAATGAGTCAACATGGCTGTTTATGAAACCTTTCACATGGCAGATGTGGGTGGCTAGTTGTGCCATCTTAATCTACACCATGTTAATAATATGGTTCCTGGAGGGTCCTTCCAGTCCAGAATTTGATGGTCCATTGATGAATCAGATCGCCACAGCTACTTGGTTCTCCTTCTCATCTCTGTTCTTTGCTCACAGAGAGAAAATCTATAGCAATTTGACACGAGTAGTTTTCATAGTGTGGCTGTTTGTTGTATTCATCCTAAACTCAAGCTACACCGCTAATCTGTCTTCGATGCTTACAATTCAACGACTGAGCCCGAATGTAACGGACATTGAAATGTTAAGGACAACCAACTCATTAGTTGGTTGTGATAACGATTCATTTGTAAGGGATTACCTGGAGAATGTTCTTCAATTAAAAAACATCGAGACAGTAGAGAGCAACTTAAAACCCGATACCTTCAGCAGCAACACGAGATTATCTGCTGTATTTCTTGAACTCCCATATGCAGAAGTTTTCATGAACGATTACTGTGAGGGATTTACCAACACCAAAGCCACCTACAGTTTTGGAGGCTTTAGCTTTGCATTTCAGAAAGGTTCTCCAATAGCGAGGAATTTCTCTAAGGTCATCCTAGATCTATTGGAGAACGGAAAAATGAGAGAACTTCGAGATCACTGGTTGAATCCGAGCAAATGTCCAACCAATACAACTTCCGATATGCCAGAAAACTTGACCATCGAGAGCTTTTGGGGCATCTTTGTAATATCCGGTGCGACTTCCACCCTCTGTCTTCTATCTTCTGCCATCTTGAAAAAGTTTGGACACCAAGCAGCATCCGGAACACGCCCTGAAAATTCCGTACAAGTGCTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0004888 GO:0005215 GO:0005216 GO:0005261 GO:0005262 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005886 GO:0006810 GO:0006811 GO:0006812 GO:0006816 GO:0007154 GO:0007165 GO:0008066 GO:0008150 GO:0008324 GO:0009719 GO:0009987 GO:0010033 GO:0010243 GO:0015075 GO:0015085 GO:0015267 GO:0015318 GO:0016020 GO:0019722 GO:0019932 GO:0022803 GO:0022838 GO:0022857 GO:0022890 GO:0023052 GO:0030001 GO:0034220 GO:0035556 GO:0038023 GO:0042221 GO:0043200 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0046873 GO:0050789 GO:0050794 GO:0050896 GO:0051179 GO:0051234 GO:0051716 GO:0055085 GO:0060089 GO:0065007 GO:0070588 GO:0070838 GO:0070887 GO:0071229 GO:0071230 GO:0071310 GO:0071417 GO:0071495 GO:0071944 GO:0072511 GO:0098655 GO:0098660 GO:0098662 GO:1901698 GO:1901699 GO:1901700 GO:1901701

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

866

Amino Acids

96.87

Weight (kDa)

5.49

Isoelectric Point (pI)

34.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peripla_BP_6	PF13458	51 - 373	1.2e-17	Periplasmic binding protein
ANF_receptor	PF01094	57 - 392	5.4e-40	Receptor family ligand binding region
Lig_chan-Glu_bd	PF10613	493 - 572	8.4e-08	Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3	PF00497	495 - 804	3.1e-11	Bacterial extracellular solute-binding proteins, family 3
Lig_chan	PF00060	587 - 834	3.4e-29	Ligand-gated ion channel

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000350)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g04591 FvH4_1g04600 FvH4_3g08210 FvH4_3g08210 FvH4_3g08211 FvH4_3g32960 FvH4_3g32961 FvH4_5g21700 FvH4_6g22211
malus_domestica	MD05G1305700.v1.1 MD05G1305800.v1.1 MD05G1305900.v1.1 MD05G1306400.v1.1
prunus_persica	Prupe.4G056200_v2.0.a1 Prupe.4G056200_v2.0.a1 Prupe.4G056600_v2.0.a1 Prupe.4G056600_v2.0.a1
pyrus_communis	pycom05g28300 pycom05g28310 pycom05g28350 pycom10g23860
rosa_chinensis	RchiOBHm_Chr5g0009861 RchiOBHm_Chr5g0009881 RchiOBHm_Chr5g0009931 RchiOBHm_Chr5g0009951 RchiOBHm_Chr5g0009981 RchiOBHm_Chr5g0055531 RchiOBHm_Chr5g0055541
rosa_laevigata	RLG00000015779 RLG00000031705 RLG00000031706 RLG00000031711 RLG00000031713 RLG00000031715 RLG00000031716 RLG00000034996
rosa_multiflora	Rmu_co8083594.1_g000001 Rmu_co8206032.1_g000001 Rmu_co8356045.1_g000001 Rmu_sc0000773.1_g000002 Rmu_sc0001709.1_g000001 Rmu_sc0001709.1_g000007 Rmu_sc0001709.1_g000012 Rmu_sc0001709.1_g000013 Rmu_sc0001709.1_g000018 Rmu_sc0001709.1_g000019 Rmu_sc0002275.1_g000004 Rmu_sc0003071.1_g000002 Rmu_sc0003071.1_g000003 Rmu_sc0003630.1_g000014 Rmu_sc0003630.1_g000017 Rmu_sc0003630.1_g000043 Rmu_sc0003630.1_g000044 Rmu_sc0004626.1_g000007
rosa_roxburghii	Rroxscaffold_1G00024770 Rroxscaffold_1G00066180 Rroxscaffold_1G00066200 Rroxscaffold_1G00066210 Rroxscaffold_1G00066240 Rroxscaffold_1G00066250 Rroxscaffold_1G00066320 Rroxscaffold_1G00066340 Rroxscaffold_3G00234120
rosa_rugosa	Rorug04G0446400 Rorug04G0446500 Rorug04G0446600 Rorug04G0447000 Rorug04G0447100 Rorug04G0447200 Rorug04G0447300 Rorug04G0447900 Rorug04G0448200
rosa_samantha	Rh2CG021600 Rh2DG021300 Rh4AG195700 Rh4BG194700 Rh5AG077900 Rh5AG078100 Rh5AG078600 Rh5AG078700 Rh5AG078900 Rh5AG079000 Rh5AG079100 Rh5AG363100 Rh5CG085300 Rh5CG085900 Rh5CG086000 Rh5CG086200 Rh5CG086400 Rh5CG086500 Rh5CG397300 Rh5DG073400 Rh5DG074000 Rh5DG074100 Rh5DG074300 Rh5DG074500 Rh5DG074600 Rh5DG129000 Rh5DG129400 Rh5DG388500
rosa_wichuraiana	Rw2G001690 Rw5G007180 Rw5G007190 Rw5G007230 Rw5G007240 Rw5G007250 Rw5G007320 Rw5G034180

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1135
AccB1I	GGYRCC	1 cut(s)	1135
AccB7I	CCANNNNNTGG	2 cut(s)	1220, 1323
AccII	CGCG	2 cut(s)	255, 1654
AccIII	TCCGGA	1 cut(s)	2564
AciI	CCGC	5 cut(s)	253, 422, 1139, 1401, 2001
AclWI	GGATC	1 cut(s)	1169
AcoI	YGGCCR	1 cut(s)	250
AcuI	CTGAAG	4 cut(s)	120, 716, 1046, 2170
AfaI	GTAC	6 cut(s)	309, 1054, 1137, 1644, 1713, 2589
AfiI	CCNNNNNNNGG	4 cut(s)	156, 1220, 1319, 1323
AhlI	ACTAGT	2 cut(s)	1049, 1597
AjnI	CCWGG	3 cut(s)	970, 1821, 2121
AjuI	GAANNNNNNNTTGG	2 cut(s)	674, 706
Alw21I	GWGCWC	1 cut(s)	2598
Alw26I	GTCTC	2 cut(s)	587, 2147
AlwI	GGATC	1 cut(s)	1169
AlwNI	CAGNNNCTG	2 cut(s)	356, 755
Ama87I	CYCGRG	2 cut(s)	714, 1295
Aor13HI	TCCGGA	1 cut(s)	2564
AoxI	GGCC	5 cut(s)	250, 443, 1020, 1292, 1566
ApeKI	GCWGC	9 cut(s)	173, 188, 269, 278, 404, 476, 685, 2190, 2558
AseI	ATTAAT	1 cut(s)	1005
Asp700I	GAANNNNTTC	3 cut(s)	799, 932, 1754
Asp718I	GGTACC	1 cut(s)	1135
AspS9I	GGNCC	3 cut(s)	1293, 1829, 1853
AsuC2I	CCSGG	2 cut(s)	1296, 1297
AsuHPI	GGTGA	1 cut(s)	1142
AvaI	CYCGRG	2 cut(s)	714, 1295
AvaII	GGWCC	2 cut(s)	1829, 1853
BanI	GGYRCC	1 cut(s)	1135
BanII	GRGCYC	1 cut(s)	2042
BauI	CACGAG	2 cut(s)	1944, 2197
BbsI	GAAGAC	3 cut(s)	1190, 2004, 2510
Bbv12I	GWGCWC	1 cut(s)	2598
BbvI	GCAGC	9 cut(s)	160, 200, 256, 265, 416, 463, 697, 2202, 2570
BccI	CCATC	9 cut(s)	464, 545, 775, 1265, 1414, 1796, 1844, 2471, 2540
BceAI	ACGGC	1 cut(s)	452
BcgI	CGANNNNNNTGC	2 cut(s)	458, 492
BciT130I	CCWGG	3 cut(s)	972, 1823, 2123
BciVI	GTATCC	1 cut(s)	1203
BclI	TGATCA	1 cut(s)	379
BcnI	CCSGG	2 cut(s)	1296, 1297
BcoDI	GTCTC	2 cut(s)	587, 2147
BcuI	ACTAGT	2 cut(s)	1049, 1597
BfaI	CTAG	9 cut(s)	774, 831, 906, 1050, 1056, 1077, 1598, 1779, 2360
BfmI	CTRYAG	3 cut(s)	12, 1930, 2287
BfuI	GTATCC	1 cut(s)	1203
BglII	AGATCT	1 cut(s)	2362
BlpI	GCTNAGC	1 cut(s)	942
BmcAI	AGTACT	1 cut(s)	1054
Bme1390I	CCNGG	5 cut(s)	972, 1296, 1297, 1823, 2123
Bme18I	GGWCC	2 cut(s)	1829, 1853
BmeT110I	CYCGRG	2 cut(s)	714, 1295
BmgT120I	GGNCC	3 cut(s)	1293, 1829, 1853
BmiI	GGNNCC	4 cut(s)	1137, 1301, 1820, 1830
BmrFI	CCNGG	5 cut(s)	972, 1296, 1297, 1823, 2123
BmsI	GCATC	5 cut(s)	1609, 1658, 2007, 2490, 2570
BpiI	GAAGAC	3 cut(s)	1190, 2004, 2510
BpmI	CTGGAG	3 cut(s)	1709, 1844, 2144
Bpu10I	CCTNAGC	1 cut(s)	1468
Bpu1102I	GCTNAGC	1 cut(s)	942
BpuEI	CTTGAG	1 cut(s)	1975
BpuMI	CCSGG	2 cut(s)	1296, 1297
BsaJI	CCNNGG	1 cut(s)	1295
BsaWI	WCCGGW	3 cut(s)	388, 2495, 2564
BsaXI	ACNNNNNCTCC	4 cut(s)	489, 519, 1119, 1149
Bsc4I	CCNNNNNNNGG	4 cut(s)	156, 1220, 1319, 1323
Bse1I	ACTGG	5 cut(s)	208, 524, 760, 1836, 2408
Bse3DI	GCAATG	1 cut(s)	1335
BseAI	TCCGGA	1 cut(s)	2564
BseBI	CCWGG	3 cut(s)	972, 1823, 2123
BseDI	CCNNGG	1 cut(s)	1295
BseGI	GGATG	4 cut(s)	676, 1980, 2355, 2561
BseLI	CCNNNNNNNGG	4 cut(s)	156, 1220, 1319, 1323
BseMI	GCAATG	1 cut(s)	1335
BseMII	CTCAG	5 cut(s)	494, 582, 642, 1482, 2027
BseNI	ACTGG	5 cut(s)	208, 524, 760, 1836, 2408
BseXI	GCAGC	9 cut(s)	160, 200, 256, 265, 416, 463, 697, 2202, 2570
BseYI	CCCAGC	2 cut(s)	1323, 1366
Bsh1236I	CGCG	2 cut(s)	255, 1654
BshFI	GGCC	5 cut(s)	252, 445, 1022, 1294, 1568
BshNI	GGYRCC	1 cut(s)	1135
BsiHKAI	GWGCWC	1 cut(s)	2598
BsiHKCI	CYCGRG	2 cut(s)	714, 1295
BsiSI	CCGG	4 cut(s)	389, 1296, 2496, 2565
BslI	CCNNNNNNNGG	4 cut(s)	156, 1220, 1319, 1323
BsmAI	GTCTC	2 cut(s)	587, 2147
BsnI	GGCC	5 cut(s)	252, 445, 1022, 1294, 1568
BsoBI	CYCGRG	2 cut(s)	714, 1295
Bsp1286I	GDGCHC	2 cut(s)	2042, 2598
Bsp13I	TCCGGA	1 cut(s)	2564
Bsp143I	GATC	8 cut(s)	39, 379, 1079, 1161, 1498, 1869, 2362, 2398
Bsp1720I	GCTNAGC	1 cut(s)	942
Bsp68I	TCGCGA	1 cut(s)	1654
BspACI	CCGC	5 cut(s)	253, 422, 1139, 1401, 2001
BspANI	GGCC	5 cut(s)	252, 445, 1022, 1294, 1568
BspCNI	CTCAG	5 cut(s)	493, 583, 643, 1481, 2028
BspEI	TCCGGA	1 cut(s)	2564
BspFNI	CGCG	2 cut(s)	255, 1654
BspHI	TCATGA	2 cut(s)	220, 2244
BspLI	GGNNCC	4 cut(s)	1137, 1301, 1820, 1830
BspPI	GGATC	1 cut(s)	1169
BspQI	GCTCTTC	1 cut(s)	990
BspT107I	GGYRCC	1 cut(s)	1135
BsrDI	GCAATG	1 cut(s)	1335
BsrI	ACTGG	5 cut(s)	208, 524, 760, 1836, 2408
BssECI	CCNNGG	1 cut(s)	1295
BssMI	GATC	8 cut(s)	39, 379, 1079, 1161, 1498, 1869, 2362, 2398
BssSI	CACGAG	2 cut(s)	1944, 2197
Bst2BI	CACGAG	2 cut(s)	1944, 2197
Bst2UI	CCWGG	3 cut(s)	972, 1823, 2123
Bst6I	CTCTTC	1 cut(s)	990
BstC8I	GCNNGC	3 cut(s)	579, 948, 1514
BstDEI	CTNAG	8 cut(s)	480, 591, 599, 651, 942, 1468, 2036, 2349
BstF5I	GGATG	4 cut(s)	676, 1980, 2355, 2561
BstFNI	CGCG	2 cut(s)	255, 1654
BstKTI	GATC	8 cut(s)	42, 382, 1082, 1164, 1501, 1872, 2365, 2401
BstMAI	GTCTC	2 cut(s)	587, 2147
BstMBI	GATC	8 cut(s)	39, 379, 1079, 1161, 1498, 1869, 2362, 2398
BstMWI	GCNNNNNNNGC	7 cut(s)	95, 275, 278, 947, 989, 1784, 2478
BstNI	CCWGG	3 cut(s)	972, 1823, 2123
BstNSI	RCATGY	1 cut(s)	950
BstSCI	CCNGG	5 cut(s)	970, 1294, 1295, 1821, 2121
BstSFI	CTRYAG	3 cut(s)	12, 1930, 2287
BstUI	CGCG	2 cut(s)	255, 1654
BstV1I	GCAGC	9 cut(s)	160, 200, 256, 265, 416, 463, 697, 2202, 2570
BstV2I	GAAGAC	3 cut(s)	1190, 2004, 2510
BstX2I	RGATCY	1 cut(s)	2362
BstYI	RGATCY	1 cut(s)	2362
BsuI	GTATCC	1 cut(s)	1203
BsuRI	GGCC	5 cut(s)	252, 445, 1022, 1294, 1568
BtgZI	GCGATG	1 cut(s)	1635
BtsCI	GGATG	4 cut(s)	676, 1980, 2355, 2561
BtsI	GCAGTG	1 cut(s)	1307
BtsIMutI	CAGTG	3 cut(s)	1307, 1335, 2401
BtuMI	TCGCGA	1 cut(s)	1654
Cac8I	GCNNGC	3 cut(s)	579, 948, 1514
CaiI	CAGNNNCTG	2 cut(s)	356, 755
CciI	TCATGA	2 cut(s)	220, 2244
Cfr13I	GGNCC	3 cut(s)	1293, 1829, 1853
Cfr9I	CCCGGG	1 cut(s)	1295
Csp6I	GTAC	6 cut(s)	308, 1053, 1136, 1643, 1712, 2588
CviAII	CATG	9 cut(s)	221, 325, 848, 947, 1544, 1737, 1761, 1804, 2245
CviQI	GTAC	6 cut(s)	308, 1053, 1136, 1643, 1712, 2588
DdeI	CTNAG	8 cut(s)	480, 591, 599, 651, 942, 1468, 2036, 2349
DpnI	GATC	8 cut(s)	41, 381, 1081, 1163, 1500, 1871, 2364, 2400
DpnII	GATC	8 cut(s)	39, 379, 1079, 1161, 1498, 1869, 2362, 2398
EaeI	YGGCCR	1 cut(s)	250
Eam1104I	CTCTTC	1 cut(s)	990
EarI	CTCTTC	1 cut(s)	990
Eco24I	GRGCYC	1 cut(s)	2042
Eco47I	GGWCC	2 cut(s)	1829, 1853
Eco57I	CTGAAG	4 cut(s)	120, 716, 1046, 2170
Eco88I	CYCGRG	2 cut(s)	714, 1295
EcoO109I	RGGNCCY	1 cut(s)	1829
EcoRI	GAATTC	2 cut(s)	920, 932
EcoRII	CCWGG	3 cut(s)	970, 1821, 2121
EcoT22I	ATGCAT	2 cut(s)	494, 514
EcoT38I	GRGCYC	1 cut(s)	2042
FaeI	CATG	9 cut(s)	224, 328, 851, 950, 1547, 1740, 1764, 1807, 2248
FatI	CATG	9 cut(s)	220, 324, 847, 946, 1543, 1736, 1760, 1803, 2244
FauI	CCCGC	2 cut(s)	429, 1394
FauNDI	CATATG	2 cut(s)	994, 2230
FbaI	TGATCA	1 cut(s)	379
FokI	GGATG	4 cut(s)	683, 1967, 2342, 2548
FriOI	GRGCYC	1 cut(s)	2042
FspBI	CTAG	9 cut(s)	774, 831, 906, 1050, 1056, 1077, 1598, 1779, 2360
GsaI	CCCAGC	2 cut(s)	1327, 1370
GsuI	CTGGAG	3 cut(s)	1709, 1844, 2144
HaeIII	GGCC	5 cut(s)	252, 445, 1022, 1294, 1568
HapII	CCGG	4 cut(s)	389, 1296, 2496, 2565
Hin1II	CATG	9 cut(s)	224, 328, 851, 950, 1547, 1740, 1764, 1807, 2248
HincII	GTYRAC	1 cut(s)	1734
HindII	GTYRAC	1 cut(s)	1734
HpaII	CCGG	4 cut(s)	389, 1296, 2496, 2565
HphI	GGTGA	1 cut(s)	1142
Hpy166II	GTNNAC	2 cut(s)	709, 1734
Hpy8I	GTNNAC	2 cut(s)	709, 1734
HpyAV	CCTTC	9 cut(s)	242, 456, 860, 922, 1022, 1070, 1842, 1903, 2194
HpyCH4V	TGCA	9 cut(s)	188, 404, 492, 512, 950, 1117, 1649, 2234, 2312
HpyF10VI	GCNNNNNNNGC	7 cut(s)	95, 275, 278, 947, 989, 1784, 2478
HpyF3I	CTNAG	8 cut(s)	480, 591, 599, 651, 942, 1468, 2036, 2349
Hsp92II	CATG	9 cut(s)	224, 328, 851, 950, 1547, 1740, 1764, 1807, 2248
Kpn2I	TCCGGA	1 cut(s)	2564
KpnI	GGTACC	1 cut(s)	1139
Ksp22I	TGATCA	1 cut(s)	379
Kzo9I	GATC	8 cut(s)	39, 379, 1079, 1161, 1498, 1869, 2362, 2398
LguI	GCTCTTC	1 cut(s)	990
LmnI	GCTCC	2 cut(s)	128, 1368
Lsp1109I	GCAGC	9 cut(s)	160, 200, 256, 265, 416, 463, 697, 2202, 2570
LweI	GCATC	5 cut(s)	1609, 1658, 2007, 2490, 2570
MaeI	CTAG	9 cut(s)	774, 831, 906, 1050, 1056, 1077, 1598, 1779, 2360
MaeIII	GTNAC	3 cut(s)	346, 751, 2047
MalI	GATC	8 cut(s)	41, 381, 1081, 1163, 1500, 1871, 2364, 2400
MboI	GATC	8 cut(s)	39, 379, 1079, 1161, 1498, 1869, 2362, 2398
MfeI	CAATTG	1 cut(s)	618
MflI	RGATCY	1 cut(s)	2362
MhlI	GDGCHC	2 cut(s)	2042, 2598
MlyI	GAGTC	4 cut(s)	589, 911, 1700, 1739
MmeI	TCCRAC	2 cut(s)	672, 2450
Mph1103I	ATGCAT	2 cut(s)	494, 514
MroI	TCCGGA	1 cut(s)	2564
MroXI	GAANNNNTTC	3 cut(s)	799, 932, 1754
MslI	CAYNNNNRTG	1 cut(s)	2060
MspA1I	CMGCKG	4 cut(s)	191, 688, 1141, 1401
MspI	CCGG	4 cut(s)	389, 1296, 2496, 2565
MspR9I	CCNGG	5 cut(s)	972, 1296, 1297, 1823, 2123
MunI	CAATTG	1 cut(s)	618
MvaI	CCWGG	3 cut(s)	972, 1823, 2123
MvnI	CGCG	2 cut(s)	255, 1654
MwoI	GCNNNNNNNGC	7 cut(s)	95, 275, 278, 947, 989, 1784, 2478
NciI	CCSGG	2 cut(s)	1296, 1297
NdeI	CATATG	2 cut(s)	994, 2230
NdeII	GATC	8 cut(s)	39, 379, 1079, 1161, 1498, 1869, 2362, 2398
NlaIII	CATG	9 cut(s)	224, 328, 851, 950, 1547, 1740, 1764, 1807, 2248
NlaIV	GGNNCC	4 cut(s)	1137, 1301, 1820, 1830
NruI	TCGCGA	1 cut(s)	1654
NsiI	ATGCAT	2 cut(s)	494, 514
NspI	RCATGY	1 cut(s)	950
PaeI	GCATGC	1 cut(s)	950
PagI	TCATGA	2 cut(s)	220, 2244
PciSI	GCTCTTC	1 cut(s)	990
PdmI	GAANNNNTTC	3 cut(s)	799, 932, 1754
PfeI	GAWTC	9 cut(s)	245, 800, 879, 1032, 1145, 1155, 1864, 2102, 2410
PflMI	CCANNNNNTGG	2 cut(s)	1220, 1323
PfoI	TCCNGGA	1 cut(s)	1821
PleI	GAGTC	4 cut(s)	589, 910, 1699, 1738
PpsI	GAGTC	4 cut(s)	589, 910, 1699, 1738
PpuMI	RGGWCCY	1 cut(s)	1829
PshBI	ATTAAT	1 cut(s)	1005
Psp5II	RGGWCCY	1 cut(s)	1829
Psp6I	CCWGG	3 cut(s)	970, 1821, 2121
PspFI	CCCAGC	2 cut(s)	1323, 1366
PspGI	CCWGG	3 cut(s)	970, 1821, 2121
PspN4I	GGNNCC	4 cut(s)	1137, 1301, 1820, 1830
PspPI	GGNCC	3 cut(s)	1293, 1829, 1853
PspPPI	RGGWCCY	1 cut(s)	1829
PsrI	GAACNNNNNNTAC	2 cut(s)	291, 323
PstNI	CAGNNNCTG	2 cut(s)	356, 755
PsuI	RGATCY	1 cut(s)	2362
PvuII	CAGCTG	2 cut(s)	191, 688
RruI	TCGCGA	1 cut(s)	1654
RsaI	GTAC	6 cut(s)	309, 1054, 1137, 1644, 1713, 2589
RsaNI	GTAC	6 cut(s)	308, 1053, 1136, 1643, 1712, 2588
RseI	CAYNNNNRTG	1 cut(s)	2060
SapI	GCTCTTC	1 cut(s)	990
Sau3AI	GATC	8 cut(s)	39, 379, 1079, 1161, 1498, 1869, 2362, 2398
Sau96I	GGNCC	3 cut(s)	1293, 1829, 1853
ScaI	AGTACT	1 cut(s)	1054
SchI	GAGTC	4 cut(s)	589, 911, 1700, 1739
ScrFI	CCNGG	5 cut(s)	972, 1296, 1297, 1823, 2123
SduI	GDGCHC	2 cut(s)	2042, 2598
SfaNI	GCATC	5 cut(s)	1609, 1658, 2007, 2490, 2570
SfcI	CTRYAG	3 cut(s)	12, 1930, 2287
SinI	GGWCC	2 cut(s)	1829, 1853
SmaI	CCCGGG	1 cut(s)	1297
SmiMI	CAYNNNNRTG	1 cut(s)	2060
SmlI	CTYRAG	1 cut(s)	1990
SmoI	CTYRAG	1 cut(s)	1990
SpeI	ACTAGT	2 cut(s)	1049, 1597
SphI	GCATGC	1 cut(s)	950
SsiI	CCGC	5 cut(s)	253, 422, 1139, 1401, 2001
SspI	AATATT	1 cut(s)	1171
SspMI	CTAG	9 cut(s)	774, 831, 906, 1050, 1056, 1077, 1598, 1779, 2360
StyD4I	CCNGG	5 cut(s)	970, 1294, 1295, 1821, 2121
TaqI	TCGA	6 cut(s)	241, 988, 2015, 2151, 2395, 2466
TatI	WGTACW	2 cut(s)	1052, 1642
TauI	GCSGC	1 cut(s)	255
TfiI	GAWTC	9 cut(s)	245, 800, 879, 1032, 1145, 1155, 1864, 2102, 2410
TscAI	CASTG	3 cut(s)	1314, 1342, 2408
TseI	GCWGC	9 cut(s)	173, 188, 269, 278, 404, 476, 685, 2190, 2558
TspGWI	ACGGA	3 cut(s)	2066, 2391, 2575
TspMI	CCCGGG	1 cut(s)	1295
TspRI	CASTG	3 cut(s)	1314, 1342, 2408
Van91I	CCANNNNNTGG	2 cut(s)	1220, 1323
VpaK11BI	GGWCC	2 cut(s)	1829, 1853
VspI	ATTAAT	1 cut(s)	1005
XceI	RCATGY	1 cut(s)	950
XmaI	CCCGGG	1 cut(s)	1295
XmnI	GAANNNNTTC	3 cut(s)	799, 932, 1754
XspI	CTAG	9 cut(s)	774, 831, 906, 1050, 1056, 1077, 1598, 1779, 2360
ZrmI	AGTACT	1 cut(s)	1054
Zsp2I	ATGCAT	2 cut(s)	494, 514

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.