FvH4_4g18554

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000560
Physical Location & Seq
Reverse (-)
22415236 .. 22417184
1949 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18554.t1

Sequence Viewer

Length: 1845 bp
ATGGATGCCCAAGATATGGAGGTTGATGCCAACAAAAATGGAGTACTGAAGGTCCACTTTAAGGAAGCTCCTCGCCCCAAGCCATTCATCATAGTAGACAGGAACAGAGATCCACCTTTCACGGCCGAAGGACTTGTCACAATGTTGAATTATCATTTGCTACATTCGGCCATTGATTTGTATGGGTTAACCAGGGAAGAGGAAATGTTTCTATCAGAGATATACGAGGATAGAAATAGAGAAGACGAACTCATGGCACCTGATGTAAGGAGAGGAGTTAGAGCCAAATTTCAGACTTTTCGAAGTGATGAGGAGATTAAGGTCAGCTATGGGTCTCTTCCCATTTCGAAAGATGAATTAGATTACTTGAGGGAATACTTCAGTGTCCATTCGGCCGAAGAACTCTTCGGCCTGACTATTGAAGAACAAAGGCTTGCGTTCATATTCGAAGACCGCATGGAGAAGATGGCTAAAGAGCAGTTGGAGATTTTCCAGGCTTCTTCGTCGGGTGCACATGTATCAGAGAAGAGTGAAGGAGATGTTTCTGAACCAGGTGAGGATGAGGAACCTTTGGTCAAGCTCTGTCTGGATATGAATCCCCCTTTTTCAGCTAAGGGGTCTAGGAGAATGAAGGAGTACCACCAGAGGAATTCTGCTCTCGCGTTGTATGGGTTGACGAATAAAGAAACTGACACACTACAAATGATCACTGAGTGTCCAAAGTCAGCTTCCCTCTTGTTAGACCTAGATTCTAGTATCGGTCTAAAGGTTTCATATCAAGCACTGCAAGAAGGTGTGACACTTCAAGTCGGCACTGAAGATATTCCAATATCGGGTCGAGACATGAAATACTTGAAAAGGTTCCACAAAATTCAGTCGGCAATTGGGTTATATGGGATGACACCTCAAGAGAGAGAGTTAGTAGAACGCTTCGATTACCATGTTCGAGAAGAAGAAAAACGCCAACAAATCCAAGCGGGTCAGGATTTGGCTGAATGGTTTGAAGATGACGATCTACATGAGGGACTTGGGCAGGAGTTGGACGATGCTGATCTTGAAGACGATGACTTCGGTGACGAGGACTTTGGGGATGAAGATATGGGAGACTATGAGGAACAAGCTGATGGTGGAGACGATTTGCTAACCCATGATGGCATCCAAGTTCCTAGCATACCGGCCGACGTGACCGCCATGAGTGCATCGGCCATCGCGGATACACGAACATTGGTCAGCAAGGCCGAAGAAACATCTAATGCAATGATTCAGAACCTGAATGGATTCTCTGTCAACATGATTAGCTTGCGGCCGAAAACAAAATTTGAATACGGCCACGTGTTAGTGGAAAAGAAGTCGGCCGGAGTATGTTTTTCTCGGCCGAGAGAGCTCACGACCCAAGTCAAAGACCCCATATTCGTCACCGCTCGCATTGGCGGATACCAAGTCTCGAGGGTCCTAGTAGATGGAGGGTCGGCCGTGAATATCCTTCCTTACTCATTAATCCCTCAACTACATCTTACTAGAGGTAGCTTTGTTCCGACTGATGTTTCCGTACATGATTACATAGGTGGGGAAGCCGAAGTACACGGAGCTTTACCATTAGCTGTAACCGTGGGGAAGAAGATAGTGGAGGCCGACTCTCAACCGTTGAAAGCTTCGGCCAATGCAATTGACGCCCAGTACTATGAAGAGGATATTGGTTGTTCGGAGATTCAAAGAGAAGATGGTACTGGTCGAATTATAAGGATTACGATGCGAAGAGCACTGGCCTTAGGCGCCGATAAAGTCCACGAAGACTCGGAGCGGCCAGCATTGGCAGATCTTTTTGACCCCATTATCCATGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

615

Amino Acids

68.81

Weight (kDa)

4.62

Isoelectric Point (pI)

41.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000560)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21400 FvH4_1g29793 FvH4_2g35581 FvH4_4g18554 FvH4_6g21671
pyrus_communis pycom11g14070
rosa_chinensis RchiOBHm_Chr6g0263201
rosa_laevigata RLG00000001861 RLG00000002050 RLG00000018783 RLG00000022649 RLG00000030152
rosa_multiflora Rmu_co8107100.1_g000001 Rmu_sc0000805.1_g000009 Rmu_sc0001347.1_g000002 Rmu_sc0001896.1_g000004 Rmu_sc0002170.1_g000040 Rmu_sc0002413.1_g000005 Rmu_sc0002489.1_g000053 Rmu_sc0002942.1_g000015 Rmu_sc0003071.1_g000022 Rmu_sc0003623.1_g000021 Rmu_sc0004063.1_g000005 Rmu_sc0004574.1_g000014 Rmu_sc0004723.1_g000009 Rmu_sc0005177.1_g000013 Rmu_sc0005994.1_g000011 Rmu_sc0006163.1_g000006 Rmu_sc0006583.1_g000008 Rmu_sc0008303.1_g000005 Rmu_sc0009027.1_g000002 Rmu_sc0009027.1_g000003 Rmu_sc0011790.1_g000009 Rmu_sc0011833.1_g000005 Rmu_sc0013493.1_g000006 Rmu_sc0016701.1_g000002 Rmu_sc0022033.1_g000001 Rmu_sc0022185.1_g000001 Rmu_sc0027245.1_g000005 Rmu_sc0030292.1_g000002 Rmu_ssc0000242.1_g000003
rosa_roxburghii Rroxscaffold_1G00015200 Rroxscaffold_1G00059290 Rroxscaffold_2G00079850 Rroxscaffold_2G00079860 Rroxscaffold_2G00107520 Rroxscaffold_2G00123420 Rroxscaffold_2G00123430 Rroxscaffold_3G00265790 Rroxscaffold_5G00335960 Rroxscaffold_5G00338270 Rroxscaffold_5G00343980 Rroxscaffold_5G00343990
rosa_samantha Rh1AG036500 Rh1DG429900 Rh2AG172600 Rh2AG610900 Rh2BG180200 Rh2BG180300 Rh2BG340600 Rh2BG400300 Rh2BG468400 Rh2BG478200 Rh2BG612500 Rh2BG612600 Rh3BG290000 Rh3BG290100 Rh3BG345200 Rh3BG345300 Rh3BG359800 Rh3CG291300 Rh4CG156300 Rh4CG264600 Rh5AG430300 Rh5BG185400 Rh6DG198700 Rh7CG199900 Rh7CG200000 Rh7CG370300 Rh7CG370600 Rh7CG395500 Rh7DG405800 Rh7DG405900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1739
AccB1I GGYRCC 2 cut(s) 256, 1772
AccB7I CCANNNNNTGG 1 cut(s) 16
AccBSI CCGCTC 2 cut(s) 1421, 1801
AccI GTMKAC 1 cut(s) 96
AccII CGCG 2 cut(s) 662, 1211
AciI CCGC 8 cut(s) 454, 977, 1188, 1211, 1303, 1419, 1431, 1801
AclWI GGATC 1 cut(s) 104
AcsI RAATTY 4 cut(s) 287, 649, 870, 1316
AcuI CTGAAG 3 cut(s) 68, 364, 837
AcvI CACGTG 1 cut(s) 1333
AcyI GRCGYC 2 cut(s) 1671, 1773
AdeI CACNNNGTG 1 cut(s) 714
AfaI GTAC 6 cut(s) 45, 638, 1551, 1581, 1679, 1726
AfiI CCNNNNNNNGG 3 cut(s) 16, 61, 833
AflIII ACRYGT 2 cut(s) 514, 1332
AgsI TTSAA 9 cut(s) 148, 422, 806, 856, 1004, 1058, 1322, 1648, 1712
AjiI CACGTC 1 cut(s) 1183
AjnI CCWGG 3 cut(s) 191, 492, 550
AjuI GAANNNNNNNTTGG 4 cut(s) 712, 744, 1677, 1709
Alw21I GWGCWC 3 cut(s) 514, 1386, 1762
Alw26I GTCTC 5 cut(s) 339, 834, 1098, 1125, 1447
Alw44I GTGCAC 1 cut(s) 510
AlwI GGATC 1 cut(s) 104
AlwNI CAGNNNCTG 1 cut(s) 1270
Ama87I CYCGRG 1 cut(s) 1444
ApaLI GTGCAC 1 cut(s) 510
ApoI RAATTY 4 cut(s) 287, 649, 870, 1316
ArsI GACNNNNNNTTYG 8 cut(s) 120, 152, 1052, 1067, 1084, 1099, 1394, 1426
AseI ATTAAT 1 cut(s) 1496
Asp700I GAANNNNTTC 4 cut(s) 207, 822, 860, 1277
AspLEI GCGC 1 cut(s) 1775
AspS9I GGNCC 2 cut(s) 52, 1450
AsuHPI GGTGA 3 cut(s) 566, 1085, 1408
AsuII TTCGAA 3 cut(s) 301, 347, 447
AvaI CYCGRG 1 cut(s) 1444
AvaII GGWCC 2 cut(s) 52, 1450
AxyI CCTNAGG 1 cut(s) 1768
BaeGI GKGCMC 1 cut(s) 514
BanI GGYRCC 2 cut(s) 256, 1772
BanII GRGCYC 1 cut(s) 1386
BarI GAAGNNNNNNTAC 2 cut(s) 1563, 1595
BbrPI CACGTG 1 cut(s) 1333
BbsI GAAGAC 4 cut(s) 249, 456, 1065, 1797
Bbv12I GWGCWC 3 cut(s) 514, 1386, 1762
BccI CCATC 6 cut(s) 460, 1118, 1145, 1214, 1454, 1715
BceAI ACGGC 3 cut(s) 138, 1342, 1457
BciT130I CCWGG 3 cut(s) 193, 494, 552
BciVI GTATCC 2 cut(s) 1207, 1427
BclI TGATCA 1 cut(s) 705
BcoDI GTCTC 5 cut(s) 339, 834, 1098, 1125, 1447
BfaI CTAG 6 cut(s) 621, 746, 753, 1167, 1454, 1518
BfoI RGCGCY 1 cut(s) 1776
BfuI GTATCC 2 cut(s) 1207, 1427
BglII AGATCT 1 cut(s) 1816
BisI GCNGC 2 cut(s) 1304, 1802
BlsI GCNGC 2 cut(s) 1305, 1803
BmcAI AGTACT 2 cut(s) 45, 1679
Bme1390I CCNGG 3 cut(s) 193, 494, 552
Bme18I GGWCC 2 cut(s) 52, 1450
BmeT110I CYCGRG 1 cut(s) 1444
BmgBI CACGTC 1 cut(s) 1183
BmgT120I GGNCC 2 cut(s) 52, 1450
BmiI GGNNCC 5 cut(s) 258, 567, 863, 1451, 1774
BmrFI CCNGG 3 cut(s) 193, 494, 552
BmrI ACTGGG 1 cut(s) 1669
BmsI GCATC 5 cut(s) 16, 1036, 1164, 1208, 1740
BmuI ACTGGG 1 cut(s) 1669
BoxI GACNNNNGTC 1 cut(s) 1394
BpiI GAAGAC 4 cut(s) 249, 456, 1065, 1797
BplI GAGNNNNNCTC 2 cut(s) 1619, 1651
Bpu10I CCTNAGC 1 cut(s) 612
Bpu14I TTCGAA 3 cut(s) 301, 347, 447
BpuEI CTTGAG 2 cut(s) 388, 891
BsaAI YACGTR 1 cut(s) 1333
BsaBI GATNNNNATC 3 cut(s) 594, 1011, 1050
BsaHI GRCGYC 2 cut(s) 1671, 1773
BsaI GGTCTC 1 cut(s) 339
BsaJI CCNNGG 2 cut(s) 192, 1608
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 61, 833
Bse118I RCCGGY 1 cut(s) 1174
Bse1I ACTGG 3 cut(s) 1675, 1732, 1767
Bse21I CCTNAGG 1 cut(s) 1768
Bse3DI GCAATG 1 cut(s) 1263
Bse8I GATNNNNATC 3 cut(s) 594, 1011, 1050
BseBI CCWGG 3 cut(s) 193, 494, 552
BseDI CCNNGG 2 cut(s) 192, 1608
BseGI GGATG 5 cut(s) 10, 565, 903, 1096, 1155
BseJI GATNNNNATC 3 cut(s) 594, 1011, 1050
BseLI CCNNNNNNNGG 3 cut(s) 16, 61, 833
BseMI GCAATG 1 cut(s) 1263
BseMII CTCAG 1 cut(s) 702
BseNI ACTGG 3 cut(s) 1675, 1732, 1767
BseRI GAGGAG 3 cut(s) 60, 288, 326
BseSI GKGCMC 1 cut(s) 514
BseX3I CGGCCG 7 cut(s) 123, 393, 1176, 1304, 1353, 1373, 1470
Bsh1236I CGCG 2 cut(s) 662, 1211
Bsh1285I CGRYCG 7 cut(s) 126, 396, 1179, 1307, 1356, 1376, 1473
BshNI GGYRCC 2 cut(s) 256, 1772
BsiEI CGRYCG 7 cut(s) 126, 396, 1179, 1307, 1356, 1376, 1473
BsiHKAI GWGCWC 3 cut(s) 514, 1386, 1762
BsiHKCI CYCGRG 1 cut(s) 1444
BsiSI CCGG 2 cut(s) 1175, 1356
BslFI GGGAC 1 cut(s) 1038
BslI CCNNNNNNNGG 3 cut(s) 16, 61, 833
BsmAI GTCTC 5 cut(s) 339, 834, 1098, 1125, 1447
BsmBI CGTCTC 1 cut(s) 1125
BsmFI GGGAC 1 cut(s) 1038
Bso31I GGTCTC 1 cut(s) 339
BsoBI CYCGRG 1 cut(s) 1444
Bsp119I TTCGAA 3 cut(s) 301, 347, 447
Bsp1286I GDGCHC 3 cut(s) 514, 1386, 1762
Bsp143I GATC 5 cut(s) 109, 705, 1012, 1051, 1816
BspACI CCGC 8 cut(s) 454, 977, 1188, 1211, 1303, 1419, 1431, 1801
BspCNI CTCAG 1 cut(s) 703
BspFNI CGCG 2 cut(s) 662, 1211
BspLI GGNNCC 5 cut(s) 258, 567, 863, 1451, 1774
BspPI GGATC 1 cut(s) 104
BspQI GCTCTTC 1 cut(s) 1750
BspT104I TTCGAA 3 cut(s) 301, 347, 447
BspT107I GGYRCC 2 cut(s) 256, 1772
BspTNI GGTCTC 1 cut(s) 339
BsrBI CCGCTC 2 cut(s) 1421, 1801
BsrDI GCAATG 1 cut(s) 1263
BsrFI RCCGGY 1 cut(s) 1174
BsrI ACTGG 3 cut(s) 1675, 1732, 1767
BssAI RCCGGY 1 cut(s) 1174
BssECI CCNNGG 2 cut(s) 192, 1608
BssMI GATC 5 cut(s) 109, 705, 1012, 1051, 1816
BssNI GRCGYC 2 cut(s) 1671, 1773
Bst2UI CCWGG 3 cut(s) 193, 494, 552
Bst4CI ACNGT 2 cut(s) 1609, 1644
Bst6I CTCTTC 6 cut(s) 192, 342, 410, 521, 1680, 1750
BstACI GRCGYC 2 cut(s) 1671, 1773
BstBAI YACGTR 1 cut(s) 1333
BstBI TTCGAA 3 cut(s) 301, 347, 447
BstC8I GCNNGC 4 cut(s) 435, 1301, 1423, 1806
BstDEI CTNAG 3 cut(s) 612, 711, 1768
BstDSI CCRYGG 1 cut(s) 1608
BstF5I GGATG 5 cut(s) 10, 565, 903, 1096, 1155
BstFNI CGCG 2 cut(s) 662, 1211
BstH2I RGCGCY 1 cut(s) 1776
BstHHI GCGC 1 cut(s) 1775
BstKTI GATC 5 cut(s) 112, 708, 1015, 1054, 1819
BstMAI GTCTC 5 cut(s) 339, 834, 1098, 1125, 1447
BstMBI GATC 5 cut(s) 109, 705, 1012, 1051, 1816
BstMCI CGRYCG 7 cut(s) 126, 396, 1179, 1307, 1356, 1376, 1473
BstMWI GCNNNNNNNGC 4 cut(s) 1196, 1381, 1670, 1772
BstNI CCWGG 3 cut(s) 193, 494, 552
BstNSI RCATGY 1 cut(s) 518
BstPAI GACNNNNGTC 1 cut(s) 1394
BstSCI CCNGG 3 cut(s) 191, 492, 550
BstSLI GKGCMC 1 cut(s) 514
BstUI CGCG 2 cut(s) 662, 1211
BstV2I GAAGAC 4 cut(s) 249, 456, 1065, 1797
BstX2I RGATCY 2 cut(s) 109, 1816
BstYI RGATCY 2 cut(s) 109, 1816
BstZI CGGCCG 7 cut(s) 123, 393, 1176, 1304, 1353, 1373, 1470
Bsu36I CCTNAGG 1 cut(s) 1768
BsuI GTATCC 2 cut(s) 1207, 1427
BtgI CCRYGG 1 cut(s) 1608
BtgZI GCGATG 1 cut(s) 1192
BtrI CACGTC 1 cut(s) 1183
BtsCI GGATG 5 cut(s) 10, 565, 903, 1096, 1155
BtsI GCAGTG 1 cut(s) 782
BtsIMutI CAGTG 5 cut(s) 388, 708, 782, 813, 1760
Cac8I GCNNGC 4 cut(s) 435, 1301, 1423, 1806
CaiI CAGNNNCTG 1 cut(s) 1270
CfoI GCGC 1 cut(s) 1775
Cfr10I RCCGGY 1 cut(s) 1174
Cfr13I GGNCC 2 cut(s) 52, 1450
CseI GACGC 1 cut(s) 1679
CsiI ACCWGGT 1 cut(s) 550
Csp6I GTAC 6 cut(s) 44, 637, 1550, 1580, 1678, 1725
CviQI GTAC 6 cut(s) 44, 637, 1550, 1580, 1678, 1725
DdeI CTNAG 3 cut(s) 612, 711, 1768
DinI GGCGCC 1 cut(s) 1774
DpnI GATC 5 cut(s) 111, 707, 1014, 1053, 1818
DpnII GATC 5 cut(s) 109, 705, 1012, 1051, 1816
DraIII CACNNNGTG 1 cut(s) 714
EagI CGGCCG 7 cut(s) 123, 393, 1176, 1304, 1353, 1373, 1470
Eam1104I CTCTTC 6 cut(s) 192, 342, 410, 521, 1680, 1750
EarI CTCTTC 6 cut(s) 192, 342, 410, 521, 1680, 1750
EciI GGCGGA 1 cut(s) 1446
Ecl136II GAGCTC 1 cut(s) 1384
EclXI CGGCCG 7 cut(s) 123, 393, 1176, 1304, 1353, 1373, 1470
Eco24I GRGCYC 1 cut(s) 1386
Eco31I GGTCTC 1 cut(s) 339
Eco47I GGWCC 2 cut(s) 52, 1450
Eco52I CGGCCG 7 cut(s) 123, 393, 1176, 1304, 1353, 1373, 1470
Eco53kI GAGCTC 1 cut(s) 1384
Eco57I CTGAAG 3 cut(s) 68, 364, 837
Eco72I CACGTG 1 cut(s) 1333
Eco81I CCTNAGG 1 cut(s) 1768
Eco88I CYCGRG 1 cut(s) 1444
EcoICRI GAGCTC 1 cut(s) 1384
EcoO109I RGGNCCY 1 cut(s) 1450
EcoRI GAATTC 1 cut(s) 649
EcoRII CCWGG 3 cut(s) 191, 492, 550
EcoT38I GRGCYC 1 cut(s) 1386
EgeI GGCGCC 1 cut(s) 1774
EheI GGCGCC 1 cut(s) 1774
Esp3I CGTCTC 1 cut(s) 1125
FalI AAGNNNNNCTT 2 cut(s) 41, 73
FaqI GGGAC 1 cut(s) 1038
FauI CCCGC 1 cut(s) 970
FbaI TGATCA 1 cut(s) 705
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 2 cut(s) 1304, 1802
FokI GGATG 5 cut(s) 17, 572, 910, 1103, 1142
FriOI GRGCYC 1 cut(s) 1386
Fsp4HI GCNGC 2 cut(s) 1304, 1802
FspBI CTAG 6 cut(s) 621, 746, 753, 1167, 1454, 1518
GlaI GCGC 1 cut(s) 1774
GluI GCNGC 2 cut(s) 1304, 1802
HaeII RGCGCY 1 cut(s) 1776
HapII CCGG 2 cut(s) 1175, 1356
HgaI GACGC 1 cut(s) 1679
HhaI GCGC 1 cut(s) 1775
Hin1I GRCGYC 2 cut(s) 1671, 1773
Hin6I GCGC 1 cut(s) 1773
HinP1I GCGC 1 cut(s) 1773
HincII GTYRAC 3 cut(s) 189, 675, 1288
HindII GTYRAC 3 cut(s) 189, 675, 1288
HindIII AAGCTT 1 cut(s) 1650
HinfI GANTC 7 cut(s) 595, 749, 1261, 1278, 1634, 1708, 1793
HpaI GTTAAC 1 cut(s) 189
HpaII CCGG 2 cut(s) 1175, 1356
HphI GGTGA 3 cut(s) 566, 1085, 1408
Hpy166II GTNNAC 8 cut(s) 55, 97, 189, 512, 675, 1288, 1582, 1786
Hpy188I TCNGA 8 cut(s) 217, 294, 523, 547, 1266, 1536, 1705, 1798
Hpy188III TCNNGA 8 cut(s) 587, 839, 908, 947, 983, 1055, 1387, 1444
Hpy8I GTNNAC 8 cut(s) 55, 97, 189, 512, 675, 1288, 1582, 1786
Hpy99I CGWCG 2 cut(s) 508, 1184
HpyAV CCTTC 6 cut(s) 43, 122, 527, 625, 785, 1493
HpyCH4III ACNGT 2 cut(s) 1609, 1644
HpyCH4IV ACGT 2 cut(s) 1182, 1332
HpyCH4V TGCA 5 cut(s) 512, 787, 1199, 1256, 1664
HpyF10VI GCNNNNNNNGC 4 cut(s) 1196, 1381, 1670, 1772
HpyF3I CTNAG 3 cut(s) 612, 711, 1768
HpySE526I ACGT 2 cut(s) 1182, 1332
Hsp92I GRCGYC 2 cut(s) 1671, 1773
HspAI GCGC 1 cut(s) 1773
KasI GGCGCC 1 cut(s) 1772
Ksp22I TGATCA 1 cut(s) 705
KspAI GTTAAC 1 cut(s) 189
Kzo9I GATC 5 cut(s) 109, 705, 1012, 1051, 1816
LguI GCTCTTC 1 cut(s) 1750
LmnI GCTCC 3 cut(s) 73, 1586, 1798
LweI GCATC 5 cut(s) 16, 1036, 1164, 1208, 1740
MabI ACCWGGT 1 cut(s) 550
MaeI CTAG 6 cut(s) 621, 746, 753, 1167, 1454, 1518
MaeII ACGT 2 cut(s) 1182, 1332
MaeIII GTNAC 6 cut(s) 136, 796, 1073, 1183, 1414, 1603
MalI GATC 5 cut(s) 111, 707, 1014, 1053, 1818
MbiI CCGCTC 2 cut(s) 1421, 1801
MboI GATC 5 cut(s) 109, 705, 1012, 1051, 1816
MfeI CAATTG 2 cut(s) 882, 1665
MflI RGATCY 2 cut(s) 109, 1816
MhlI GDGCHC 3 cut(s) 514, 1386, 1762
MluCI AATT 9 cut(s) 148, 287, 356, 649, 870, 882, 1316, 1665, 1734
Mly113I GGCGCC 1 cut(s) 1773
MlyI GAGTC 2 cut(s) 1628, 1787
MmeI TCCRAC 3 cut(s) 462, 1020, 1559
MroXI GAANNNNTTC 4 cut(s) 207, 822, 860, 1277
MseI TTAA 4 cut(s) 60, 188, 318, 1496
MspI CCGG 2 cut(s) 1175, 1356
MspR9I CCNGG 3 cut(s) 193, 494, 552
MunI CAATTG 2 cut(s) 882, 1665
MvaI CCWGG 3 cut(s) 193, 494, 552
MvnI CGCG 2 cut(s) 662, 1211
MwoI GCNNNNNNNGC 4 cut(s) 1196, 1381, 1670, 1772
NarI GGCGCC 1 cut(s) 1773
NdeII GATC 5 cut(s) 109, 705, 1012, 1051, 1816
NlaIV GGNNCC 5 cut(s) 258, 567, 863, 1451, 1774
NmeAIII GCCGAG 2 cut(s) 1351, 1401
NmuCI GTSAC 5 cut(s) 136, 796, 1073, 1183, 1414
NspI RCATGY 1 cut(s) 518
NspV TTCGAA 3 cut(s) 301, 347, 447
PaeR7I CTCGAG 1 cut(s) 1444
PciI ACATGT 1 cut(s) 514
PciSI GCTCTTC 1 cut(s) 1750
PdmI GAANNNNTTC 4 cut(s) 207, 822, 860, 1277
PfeI GAWTC 5 cut(s) 595, 749, 1261, 1278, 1708
PflMI CCANNNNNTGG 1 cut(s) 16
PkrI GCNGC 2 cut(s) 1305, 1803
PleI GAGTC 2 cut(s) 1628, 1787
PluTI GGCGCC 1 cut(s) 1776
PmaCI CACGTG 1 cut(s) 1333
PmlI CACGTG 1 cut(s) 1333
PpsI GAGTC 2 cut(s) 1628, 1787
Ppu21I YACGTR 1 cut(s) 1333
PpuMI RGGWCCY 1 cut(s) 1450
PscI ACATGT 1 cut(s) 514
PshAI GACNNNNGTC 1 cut(s) 1394
PshBI ATTAAT 1 cut(s) 1496
PsiI TTATAA 1 cut(s) 1739
Psp124BI GAGCTC 1 cut(s) 1386
Psp5II RGGWCCY 1 cut(s) 1450
Psp6I CCWGG 3 cut(s) 191, 492, 550
PspCI CACGTG 1 cut(s) 1333
PspGI CCWGG 3 cut(s) 191, 492, 550
PspN4I GGNNCC 5 cut(s) 258, 567, 863, 1451, 1774
PspPI GGNCC 2 cut(s) 52, 1450
PspPPI RGGWCCY 1 cut(s) 1450
PstNI CAGNNNCTG 1 cut(s) 1270
PsuI RGATCY 2 cut(s) 109, 1816
RsaI GTAC 6 cut(s) 45, 638, 1551, 1581, 1679, 1726
RsaNI GTAC 6 cut(s) 44, 637, 1550, 1580, 1678, 1725
SacI GAGCTC 1 cut(s) 1386
SapI GCTCTTC 1 cut(s) 1750
SaqAI TTAA 4 cut(s) 60, 188, 318, 1496
SatI GCNGC 2 cut(s) 1304, 1802
Sau3AI GATC 5 cut(s) 109, 705, 1012, 1051, 1816
Sau96I GGNCC 2 cut(s) 52, 1450
ScaI AGTACT 2 cut(s) 45, 1679
SchI GAGTC 2 cut(s) 1628, 1787
ScrFI CCNGG 3 cut(s) 193, 494, 552
SduI GDGCHC 3 cut(s) 514, 1386, 1762
SexAI ACCWGGT 1 cut(s) 550
SfaNI GCATC 5 cut(s) 16, 1036, 1164, 1208, 1740
SfoI GGCGCC 1 cut(s) 1774
Sfr274I CTCGAG 1 cut(s) 1444
SfuI TTCGAA 3 cut(s) 301, 347, 447
SinI GGWCC 2 cut(s) 52, 1450
SlaI CTCGAG 1 cut(s) 1444
SmlI CTYRAG 3 cut(s) 367, 906, 1444
SmoI CTYRAG 3 cut(s) 367, 906, 1444
Sse9I AATT 9 cut(s) 148, 287, 356, 649, 870, 882, 1316, 1665, 1734
SsiI CCGC 8 cut(s) 454, 977, 1188, 1211, 1303, 1419, 1431, 1801
SspDI GGCGCC 1 cut(s) 1772
SspMI CTAG 6 cut(s) 621, 746, 753, 1167, 1454, 1518
SstI GAGCTC 1 cut(s) 1386
StyD4I CCNGG 3 cut(s) 191, 492, 550
TaaI ACNGT 2 cut(s) 1609, 1644
TaiI ACGT 2 cut(s) 1185, 1335
TaqI TCGA 8 cut(s) 301, 347, 447, 838, 933, 946, 1445, 1732
TaqII GACCGA 1 cut(s) 749
TasI AATT 9 cut(s) 148, 287, 356, 649, 870, 882, 1316, 1665, 1734
TatI WGTACW 3 cut(s) 43, 1579, 1677
TauI GCSGC 2 cut(s) 1306, 1804
TfiI GAWTC 5 cut(s) 595, 749, 1261, 1278, 1708
Tru1I TTAA 4 cut(s) 60, 188, 318, 1496
Tru9I TTAA 4 cut(s) 60, 188, 318, 1496
TscAI CASTG 5 cut(s) 388, 715, 789, 820, 1767
TseFI GTSAC 5 cut(s) 136, 796, 1073, 1183, 1414
Tsp45I GTSAC 5 cut(s) 136, 796, 1073, 1183, 1414
TspDTI ATGAA 9 cut(s) 76, 369, 430, 608, 644, 762, 860, 1107, 1698
TspGWI ACGGA 2 cut(s) 1537, 1599
TspRI CASTG 5 cut(s) 388, 715, 789, 820, 1767
Van91I CCANNNNNTGG 1 cut(s) 16
VneI GTGCAC 1 cut(s) 510
VpaK11BI GGWCC 2 cut(s) 52, 1450
VspI ATTAAT 1 cut(s) 1496
XapI RAATTY 4 cut(s) 287, 649, 870, 1316
XceI RCATGY 1 cut(s) 518
XhoI CTCGAG 1 cut(s) 1444
XmiI GTMKAC 1 cut(s) 96
XmnI GAANNNNTTC 4 cut(s) 207, 822, 860, 1277
XspI CTAG 6 cut(s) 621, 746, 753, 1167, 1454, 1518
ZrmI AGTACT 2 cut(s) 45, 1679
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.