Rh5BG185400

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0000560
Physical Location & Seq
Forward (+)
20377204 .. 20382855
5652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG185400.1

Sequence Viewer

Length: 951 bp
ATGTCAACAACAACCCCAGAGCCACTTGCAGAAGATGAGAAGAAAGGAACCATTTATGTTCCTGCTGCTACAGAGAAAGCTCCAGCAACTGCATCTAACAAGCTACCCCCAGGAGTCAAGGGGAACTCTCAAGTTGAGAAGTTAAAAGTTGAAGTCCTGAGAAACAAGCTACCTCCTTTTTCTTCGAAGAGTTGGACTACCATGAAGGAATACCACAAGACACATTCGGCTTTAGCTTTGTATGGGCCAACGTGGGAAGAACTTGATACTCTGAAGGCTTTAACAGAAAACCCAGGGCTTGAGCCTTTGATGGTGCAAAGTACATCAAGTGCAGGATTGAAGATTTTATATCAGGCACAATTAGATGGTGTGGAGTTGGTGGCGGACACTGTTGATTTACCAGTATCCCAGCGAGATCTCAAGTATCTCAAGGAGTACCACAAAATGTATTCGGTTATCAGTACATATGGAGTAACTCCACAAGAGAGGAAGTTGTTGGTGAAGATGGAAGAACGCATTCGCCAACGAGAACTTGAATATGCTGCGGAGCTGGGCAAGGGAGATTCTGATTTTGAGTTGGAAGAGGAACTAGCAAACATGGACATAGACTGCAATGACACTCATCAACAAACCAGTTCTTTTGTGTGCGATGGAAAAGCCAACCAAGTAGATGGAAAATTCCATATTGAAGATGAGCAGCCAATGATTGAGGTAGAAGAAGAGCCAGAGTTTGAGAAGCCAACTGATGTTAGCTTTACAGAAGACTTCATTATCCATGATGAAGATCTCACTGGCCGCGGCAGAAGCTTTAATAGCCGTGACCATGATGAAGACCTCATTGGCCGCGGCAGAAGCTTCAATAGCCGCGACCATGGTGAAGACCTCACCGGCCACTGTAAAGGAGGTGAGATGGATGAAGGAATTGTAGCGTCATCATTGCCGAATCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

316

Amino Acids

35.41

Weight (kDa)

4.71

Isoelectric Point (pI)

46.73

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000560)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21400 FvH4_1g29793 FvH4_2g35581 FvH4_4g18554 FvH4_6g21671
pyrus_communis pycom11g14070
rosa_chinensis RchiOBHm_Chr6g0263201
rosa_laevigata RLG00000001861 RLG00000002050 RLG00000018783 RLG00000022649 RLG00000030152
rosa_multiflora Rmu_co8107100.1_g000001 Rmu_sc0000805.1_g000009 Rmu_sc0001347.1_g000002 Rmu_sc0001896.1_g000004 Rmu_sc0002170.1_g000040 Rmu_sc0002413.1_g000005 Rmu_sc0002489.1_g000053 Rmu_sc0002942.1_g000015 Rmu_sc0003071.1_g000022 Rmu_sc0003623.1_g000021 Rmu_sc0004063.1_g000005 Rmu_sc0004574.1_g000014 Rmu_sc0004723.1_g000009 Rmu_sc0005177.1_g000013 Rmu_sc0005994.1_g000011 Rmu_sc0006163.1_g000006 Rmu_sc0006583.1_g000008 Rmu_sc0008303.1_g000005 Rmu_sc0009027.1_g000002 Rmu_sc0009027.1_g000003 Rmu_sc0011790.1_g000009 Rmu_sc0011833.1_g000005 Rmu_sc0013493.1_g000006 Rmu_sc0016701.1_g000002 Rmu_sc0022033.1_g000001 Rmu_sc0022185.1_g000001 Rmu_sc0027245.1_g000005 Rmu_sc0030292.1_g000002 Rmu_ssc0000242.1_g000003
rosa_roxburghii Rroxscaffold_1G00015200 Rroxscaffold_1G00059290 Rroxscaffold_2G00079850 Rroxscaffold_2G00079860 Rroxscaffold_2G00107520 Rroxscaffold_2G00123420 Rroxscaffold_2G00123430 Rroxscaffold_3G00265790 Rroxscaffold_5G00335960 Rroxscaffold_5G00338270 Rroxscaffold_5G00343980 Rroxscaffold_5G00343990
rosa_samantha Rh1AG036500 Rh1DG429900 Rh2AG172600 Rh2AG610900 Rh2BG180200 Rh2BG180300 Rh2BG340600 Rh2BG400300 Rh2BG468400 Rh2BG478200 Rh2BG612500 Rh2BG612600 Rh3BG290000 Rh3BG290100 Rh3BG345200 Rh3BG345300 Rh3BG359800 Rh3CG291300 Rh4CG156300 Rh4CG264600 Rh5AG430300 Rh5BG185400 Rh6DG198700 Rh7CG199900 Rh7CG200000 Rh7CG370300 Rh7CG370600 Rh7CG395500 Rh7DG405800 Rh7DG405900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 798, 846, 867
AciI CCGC 7 cut(s) 383, 545, 796, 798, 844, 846, 865
AcoI YGGCCR 3 cut(s) 793, 841, 889
AcsI RAATTY 1 cut(s) 677
AcuI CTGAAG 1 cut(s) 293
AfaI GTAC 3 cut(s) 322, 437, 463
AgsI TTSAA 5 cut(s) 152, 340, 536, 689, 859
AjnI CCWGG 2 cut(s) 109, 292
AjuI GAANNNNNNNTTGG 2 cut(s) 822, 854
AluBI AGCT 8 cut(s) 80, 103, 169, 236, 550, 753, 807, 855
AluI AGCT 8 cut(s) 80, 103, 169, 236, 550, 753, 807, 855
AlwNI CAGNNNCTG 1 cut(s) 89
AoxI GGCC 4 cut(s) 245, 793, 841, 889
ApeKI GCWGC 3 cut(s) 65, 542, 697
ApoI RAATTY 1 cut(s) 677
Asp700I GAANNNNTTC 1 cut(s) 516
AspS9I GGNCC 1 cut(s) 245
AsuHPI GGTGA 4 cut(s) 511, 877, 887, 917
AsuII TTCGAA 1 cut(s) 185
BbsI GAAGAC 3 cut(s) 768, 837, 885
BbvI GCAGC 3 cut(s) 52, 529, 709
BccI CCATC 6 cut(s) 304, 359, 499, 644, 665, 904
BceAI ACGGC 1 cut(s) 801
BciT130I CCWGG 2 cut(s) 111, 294
BciVI GTATCC 1 cut(s) 415
BfaI CTAG 1 cut(s) 590
BfmI CTRYAG 1 cut(s) 69
BfuI GTATCC 1 cut(s) 415
BglII AGATCT 2 cut(s) 415, 784
BisI GCNGC 8 cut(s) 66, 543, 698, 796, 799, 844, 847, 865
BlsI GCNGC 8 cut(s) 67, 544, 699, 797, 800, 845, 848, 866
Bme1390I CCNGG 2 cut(s) 111, 294
BmgT120I GGNCC 1 cut(s) 245
BmiI GGNNCC 1 cut(s) 49
BmrFI CCNGG 2 cut(s) 111, 294
BmsI GCATC 1 cut(s) 101
BpiI GAAGAC 3 cut(s) 768, 837, 885
BpmI CTGGAG 1 cut(s) 66
Bpu14I TTCGAA 1 cut(s) 185
BpuEI CTTGAG 4 cut(s) 114, 320, 404, 413
BsaBI GATNNNNATC 1 cut(s) 783
BsaJI CCNNGG 6 cut(s) 109, 292, 293, 796, 844, 871
Bse118I RCCGGY 1 cut(s) 887
Bse1I ACTGG 3 cut(s) 401, 633, 796
Bse3DI GCAATG 2 cut(s) 619, 935
Bse8I GATNNNNATC 1 cut(s) 783
BseBI CCWGG 2 cut(s) 111, 294
BseDI CCNNGG 6 cut(s) 109, 292, 293, 796, 844, 871
BseGI GGATG 1 cut(s) 919
BseJI GATNNNNATC 1 cut(s) 783
BseMI GCAATG 2 cut(s) 619, 935
BseMII CTCAG 1 cut(s) 149
BseNI ACTGG 3 cut(s) 401, 633, 796
BseXI GCAGC 3 cut(s) 52, 529, 709
BseYI CCCAGC 2 cut(s) 408, 550
BsgI GTGCAG 1 cut(s) 351
Bsh1236I CGCG 3 cut(s) 798, 846, 867
BshFI GGCC 4 cut(s) 247, 795, 843, 891
BsiSI CCGG 1 cut(s) 888
BsmI GAATGC 1 cut(s) 516
BsnI GGCC 4 cut(s) 247, 795, 843, 891
Bsp119I TTCGAA 1 cut(s) 185
Bsp143I GATC 2 cut(s) 415, 784
Bsp19I CCATGG 1 cut(s) 871
BspACI CCGC 7 cut(s) 383, 545, 796, 798, 844, 846, 865
BspANI GGCC 4 cut(s) 247, 795, 843, 891
BspCNI CTCAG 1 cut(s) 150
BspFNI CGCG 3 cut(s) 798, 846, 867
BspLI GGNNCC 1 cut(s) 49
BspQI GCTCTTC 1 cut(s) 714
BspT104I TTCGAA 1 cut(s) 185
BsrDI GCAATG 2 cut(s) 619, 935
BsrFI RCCGGY 1 cut(s) 887
BsrI ACTGG 3 cut(s) 401, 633, 796
BssAI RCCGGY 1 cut(s) 887
BssECI CCNNGG 6 cut(s) 109, 292, 293, 796, 844, 871
BssMI GATC 2 cut(s) 415, 784
BssT1I CCWWGG 1 cut(s) 871
Bst2UI CCWGG 2 cut(s) 111, 294
Bst4CI ACNGT 2 cut(s) 391, 896
Bst6I CTCTTC 3 cut(s) 182, 576, 714
BstBI TTCGAA 1 cut(s) 185
BstDEI CTNAG 1 cut(s) 158
BstDSI CCRYGG 3 cut(s) 796, 844, 871
BstF5I GGATG 1 cut(s) 919
BstFNI CGCG 3 cut(s) 798, 846, 867
BstKTI GATC 2 cut(s) 418, 787
BstMBI GATC 2 cut(s) 415, 784
BstMWI GCNNNNNNNGC 4 cut(s) 804, 813, 852, 861
BstNI CCWGG 2 cut(s) 111, 294
BstSCI CCNGG 2 cut(s) 109, 292
BstSFI CTRYAG 1 cut(s) 69
BstUI CGCG 3 cut(s) 798, 846, 867
BstV1I GCAGC 3 cut(s) 52, 529, 709
BstV2I GAAGAC 3 cut(s) 768, 837, 885
BstX2I RGATCY 2 cut(s) 415, 784
BstXI CCANNNNNNTGG 1 cut(s) 671
BstYI RGATCY 2 cut(s) 415, 784
BsuI GTATCC 1 cut(s) 415
BsuRI GGCC 4 cut(s) 247, 795, 843, 891
BtgI CCRYGG 3 cut(s) 796, 844, 871
BtgZI GCGATG 1 cut(s) 663
BtsCI GGATG 1 cut(s) 919
BtsIMutI CAGTG 3 cut(s) 387, 789, 892
CaiI CAGNNNCTG 1 cut(s) 89
Cfr10I RCCGGY 1 cut(s) 887
Cfr13I GGNCC 1 cut(s) 245
Cfr42I CCGCGG 2 cut(s) 799, 847
CseI GACGC 1 cut(s) 918
Csp6I GTAC 3 cut(s) 321, 436, 462
CviAII CATG 5 cut(s) 202, 598, 776, 824, 872
CviQI GTAC 3 cut(s) 321, 436, 462
DdeI CTNAG 1 cut(s) 158
DpnI GATC 2 cut(s) 417, 786
DpnII GATC 2 cut(s) 415, 784
EaeI YGGCCR 3 cut(s) 793, 841, 889
Eam1104I CTCTTC 3 cut(s) 182, 576, 714
EarI CTCTTC 3 cut(s) 182, 576, 714
EciI GGCGGA 1 cut(s) 398
Eco130I CCWWGG 1 cut(s) 871
Eco57I CTGAAG 1 cut(s) 293
EcoRII CCWGG 2 cut(s) 109, 292
EcoT14I CCWWGG 1 cut(s) 871
ErhI CCWWGG 1 cut(s) 871
FaeI CATG 5 cut(s) 205, 601, 779, 827, 875
FatI CATG 5 cut(s) 201, 597, 775, 823, 871
FauNDI CATATG 1 cut(s) 466
Fnu4HI GCNGC 8 cut(s) 66, 543, 698, 796, 799, 844, 847, 865
FokI GGATG 1 cut(s) 926
Fsp4HI GCNGC 8 cut(s) 66, 543, 698, 796, 799, 844, 847, 865
FspBI CTAG 1 cut(s) 590
GluI GCNGC 8 cut(s) 66, 543, 698, 796, 799, 844, 847, 865
GsaI CCCAGC 2 cut(s) 412, 554
GsuI CTGGAG 1 cut(s) 66
HaeIII GGCC 4 cut(s) 247, 795, 843, 891
HapII CCGG 1 cut(s) 888
HgaI GACGC 1 cut(s) 918
Hin1II CATG 5 cut(s) 205, 601, 779, 827, 875
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HindIII AAGCTT 2 cut(s) 805, 853
HinfI GANTC 3 cut(s) 114, 563, 943
HpaII CCGG 1 cut(s) 888
HphI GGTGA 4 cut(s) 511, 877, 887, 917
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 2 cut(s) 273, 568
Hpy188III TCNNGA 1 cut(s) 157
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 3 cut(s) 199, 268, 911
HpyCH4III ACNGT 2 cut(s) 391, 896
HpyCH4IV ACGT 1 cut(s) 251
HpyCH4V TGCA 5 cut(s) 29, 92, 316, 332, 612
HpyF10VI GCNNNNNNNGC 4 cut(s) 804, 813, 852, 861
HpyF3I CTNAG 1 cut(s) 158
HpySE526I ACGT 1 cut(s) 251
Hsp92II CATG 5 cut(s) 205, 601, 779, 827, 875
KspI CCGCGG 2 cut(s) 799, 847
Kzo9I GATC 2 cut(s) 415, 784
LguI GCTCTTC 1 cut(s) 714
LmnI GCTCC 2 cut(s) 85, 547
Lsp1109I GCAGC 3 cut(s) 52, 529, 709
LweI GCATC 1 cut(s) 101
MaeI CTAG 1 cut(s) 590
MaeII ACGT 1 cut(s) 251
MaeIII GTNAC 2 cut(s) 472, 818
MalI GATC 2 cut(s) 417, 786
MboI GATC 2 cut(s) 415, 784
MflI RGATCY 2 cut(s) 415, 784
MluCI AATT 3 cut(s) 359, 677, 921
MlyI GAGTC 1 cut(s) 123
MmeI TCCRAC 2 cut(s) 173, 558
MnlI CCTC 7 cut(s) 183, 480, 577, 703, 845, 893, 896
MroXI GAANNNNTTC 1 cut(s) 516
MseI TTAA 3 cut(s) 143, 281, 810
MspA1I CMGCKG 2 cut(s) 798, 846
MspI CCGG 1 cut(s) 888
MspR9I CCNGG 2 cut(s) 111, 294
Mva1269I GAATGC 1 cut(s) 516
MvaI CCWGG 2 cut(s) 111, 294
MvnI CGCG 3 cut(s) 798, 846, 867
MwoI GCNNNNNNNGC 4 cut(s) 804, 813, 852, 861
NcoI CCATGG 1 cut(s) 871
NdeI CATATG 1 cut(s) 466
NdeII GATC 2 cut(s) 415, 784
NlaIII CATG 5 cut(s) 205, 601, 779, 827, 875
NlaIV GGNNCC 1 cut(s) 49
NmuCI GTSAC 1 cut(s) 818
NspV TTCGAA 1 cut(s) 185
PasI CCCWGGG 1 cut(s) 293
PciSI GCTCTTC 1 cut(s) 714
PctI GAATGC 1 cut(s) 516
PdmI GAANNNNTTC 1 cut(s) 516
PfeI GAWTC 2 cut(s) 563, 943
PkrI GCNGC 8 cut(s) 67, 544, 699, 797, 800, 845, 848, 866
PleI GAGTC 1 cut(s) 122
PpsI GAGTC 1 cut(s) 122
Psp6I CCWGG 2 cut(s) 109, 292
PspFI CCCAGC 2 cut(s) 408, 550
PspGI CCWGG 2 cut(s) 109, 292
PspN4I GGNNCC 1 cut(s) 49
PspPI GGNCC 1 cut(s) 245
PstNI CAGNNNCTG 1 cut(s) 89
PsuI RGATCY 2 cut(s) 415, 784
RsaI GTAC 3 cut(s) 322, 437, 463
RsaNI GTAC 3 cut(s) 321, 436, 462
SacII CCGCGG 2 cut(s) 799, 847
SapI GCTCTTC 1 cut(s) 714
SaqAI TTAA 3 cut(s) 143, 281, 810
SatI GCNGC 8 cut(s) 66, 543, 698, 796, 799, 844, 847, 865
Sau3AI GATC 2 cut(s) 415, 784
Sau96I GGNCC 1 cut(s) 245
SchI GAGTC 1 cut(s) 123
ScrFI CCNGG 2 cut(s) 111, 294
SfaNI GCATC 1 cut(s) 101
SfcI CTRYAG 1 cut(s) 69
Sfr303I CCGCGG 2 cut(s) 799, 847
SfuI TTCGAA 1 cut(s) 185
SgrBI CCGCGG 2 cut(s) 799, 847
SmlI CTYRAG 4 cut(s) 129, 299, 419, 428
SmoI CTYRAG 4 cut(s) 129, 299, 419, 428
Sse9I AATT 3 cut(s) 359, 677, 921
SsiI CCGC 7 cut(s) 383, 545, 796, 798, 844, 846, 865
SspMI CTAG 1 cut(s) 590
StyD4I CCNGG 2 cut(s) 109, 292
StyI CCWWGG 1 cut(s) 871
TaaI ACNGT 2 cut(s) 391, 896
TaiI ACGT 1 cut(s) 254
TaqI TCGA 1 cut(s) 185
TasI AATT 3 cut(s) 359, 677, 921
TatI WGTACW 2 cut(s) 320, 461
TauI GCSGC 5 cut(s) 798, 801, 846, 849, 867
TfiI GAWTC 2 cut(s) 563, 943
Tru1I TTAA 3 cut(s) 143, 281, 810
Tru9I TTAA 3 cut(s) 143, 281, 810
TscAI CASTG 3 cut(s) 394, 796, 899
TseFI GTSAC 1 cut(s) 818
TseI GCWGC 3 cut(s) 65, 542, 697
Tsp45I GTSAC 1 cut(s) 818
TspDTI ATGAA 5 cut(s) 218, 757, 795, 843, 930
TspRI CASTG 3 cut(s) 394, 796, 899
XapI RAATTY 1 cut(s) 677
XmnI GAANNNNTTC 1 cut(s) 516
XspI CTAG 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.