Rmu_sc0003071.1_g000022

Uncharacterized protein K02A2.6-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003071.1
OG0000560
Physical Location & Seq
Forward (+)
65562 .. 69597
4036 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003071.1_g000022.1.cds

Sequence Viewer

Length: 2313 bp
atggatagcgccggctttgccgcgttgcctctatgctggctgtcggacgggcacttcaacgccctgggctgggatcgggagatgttgggacggcgcgatcagacggcggccaggatctgggagcatctgggtaaccgaggaggcagcgaggatctggaagcatgtgggcgaccagcgcggcttgtgggtctcggggataatgctttggcaatggctcgagggtctcaggacagtgctggcttgtggaagagattggcaacgcggctcttaggatcgaggctagtttttgcggcgggacttatgggcggcgcgagtgttgatggcaggtttggaaggcttttcttgacgtctgtggtggtttctttgcagggacggcgcgatcagacggtgaccaggatctgggagcatctgggtaaccgaggaggcagtgaggatctggaagcatgtgggcgaccagcgcggcttgtgggtctcggggatagtgctttggcaatggctcgagggtctcagggcagtgctggcttgtggaagagattggcaacgcggctcttaggatcgaggctagtttttgcggcgggacttatgggcggcgcggctcgtggtgaggctggcttcggggctgtactcatgggcagcgctgcacaggcatctgtggacgtgatggaatggtggagtgggcttgggtcaactcggttttgctggacattgagtagggctcttctctttgggtctggcctattgggccttctaattttggctacggctgtacgtgaacttgctttttccaggaaggtagaaggggcaaaacgtgagcaagaaaggctgagaaaggttcaagaacaaggtgaacatgagaagtcccagttgagaaagggaataaacaatcaggggcattggcatgaccattacgagagaagtcatgaatcaaatgaaagattgcaacatggacaccggggttcagaagaaactaggagaggtagctcggcatcaacttctcaggttgctacaacttcagtgccaagattgaaggtagtacagaaagaggcttcaccggttagtctagaaggccacaagctattgaagagggatgtatcttcaaaagtccgagtgcatgtgcatttaaatccaccattcagtcctaaaggtttggagaaaatgaaaagatatcataagaaacactcagctatcaagctttatggtttgcttgaggaagaggaaagactatttttagagttgtctacggatgaagacttcttggcacggcctattattagccgagggttaaaagctatgttccaagcagcatgcgaaaattcttctccaacagtgtgcacaccgaaggatagatcagccctaccagtgtctgaggaagatttattgtatttaacggagtaccaccagcgctattcggctgagaagttatatggccttactgaggaagaggaagaactggcagatgcattcgattggtacatattccagaagaagagtgaggcagggataaaagctacagataattctcgatctgagcaggtgatggttgaagtgctgaggaataaattacctcctttttcagctaaagatttgactaccatgaaagagtatcacaagaagcagtcggccttagctttatatgggccgacatgggaagagttagatacccttaaggctttggcagataatcctgagctcgagcctataatggtacagaccgaagtaaacaaggaagactctgattttgagttgagtgatgggccagaagatatggttatagactgcaatgagattcagcaaggaaccatctttcatgcggccgaggttgaaaattcacaaccccaagttcagagtaagaaatttgaaattgaagataaacaagcaatgattgatgttgaagatcaagagggtgacaagcgcaatgaagattttgttgagggtcaggttttcgaaggaggaccaaagtacaacaatcatgggccggatcagccgaatacaatggaaggagatgttgaagatagaattttccctagagtttcaattcaggaacagcctcaagctcacttccagaagccaacgccagagatggtaaagcatataaaacctctgtacatcacggtatcctttgaaggatatccagtttcaaaagttttggttgatggtggggctacagtgaatgtcatccctatgtccatggtgaccaagcttaagaaagcaaagaaggacattttgcctgcagacttgaggaatcatcatcaccatcactattttcaccatgtcaatgccttcatgagctcatctacaccatcctcaacttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 rve

Protein Analysis

770

Amino Acids

85.64

Weight (kDa)

6.3

Isoelectric Point (pI)

48.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000560)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21400 FvH4_1g29793 FvH4_2g35581 FvH4_4g18554 FvH4_6g21671
pyrus_communis pycom11g14070
rosa_chinensis RchiOBHm_Chr6g0263201
rosa_laevigata RLG00000001861 RLG00000002050 RLG00000018783 RLG00000022649 RLG00000030152
rosa_multiflora Rmu_co8107100.1_g000001 Rmu_sc0000805.1_g000009 Rmu_sc0001347.1_g000002 Rmu_sc0001896.1_g000004 Rmu_sc0002170.1_g000040 Rmu_sc0002413.1_g000005 Rmu_sc0002489.1_g000053 Rmu_sc0002942.1_g000015 Rmu_sc0003071.1_g000022 Rmu_sc0003623.1_g000021 Rmu_sc0004063.1_g000005 Rmu_sc0004574.1_g000014 Rmu_sc0004723.1_g000009 Rmu_sc0005177.1_g000013 Rmu_sc0005994.1_g000011 Rmu_sc0006163.1_g000006 Rmu_sc0006583.1_g000008 Rmu_sc0008303.1_g000005 Rmu_sc0009027.1_g000002 Rmu_sc0009027.1_g000003 Rmu_sc0011790.1_g000009 Rmu_sc0011833.1_g000005 Rmu_sc0013493.1_g000006 Rmu_sc0016701.1_g000002 Rmu_sc0022033.1_g000001 Rmu_sc0022185.1_g000001 Rmu_sc0027245.1_g000005 Rmu_sc0030292.1_g000002 Rmu_ssc0000242.1_g000003
rosa_roxburghii Rroxscaffold_1G00015200 Rroxscaffold_1G00059290 Rroxscaffold_2G00079850 Rroxscaffold_2G00079860 Rroxscaffold_2G00107520 Rroxscaffold_2G00123420 Rroxscaffold_2G00123430 Rroxscaffold_3G00265790 Rroxscaffold_5G00335960 Rroxscaffold_5G00338270 Rroxscaffold_5G00343980 Rroxscaffold_5G00343990
rosa_samantha Rh1AG036500 Rh1DG429900 Rh2AG172600 Rh2AG610900 Rh2BG180200 Rh2BG180300 Rh2BG340600 Rh2BG400300 Rh2BG468400 Rh2BG478200 Rh2BG612500 Rh2BG612600 Rh3BG290000 Rh3BG290100 Rh3BG345200 Rh3BG345300 Rh3BG359800 Rh3CG291300 Rh4CG156300 Rh4CG264600 Rh5AG430300 Rh5BG185400 Rh6DG198700 Rh7CG199900 Rh7CG200000 Rh7CG370300 Rh7CG370600 Rh7CG395500 Rh7DG405800 Rh7DG405900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1522
AatII GACGTC 1 cut(s) 350
Acc36I ACCTGC 2 cut(s) 315, 1522
AccB7I CCANNNNNTGG 2 cut(s) 117, 399
AccI GTMKAC 1 cut(s) 1238
AccII CGCG 9 cut(s) 23, 96, 178, 262, 311, 378, 460, 544, 593
AclWI GGATC 8 cut(s) 81, 122, 159, 280, 404, 441, 562, 1986
AcoI YGGCCR 2 cut(s) 108, 1812
AcsI RAATTY 4 cut(s) 1312, 1825, 1853, 2016
AcuI CTGAAG 1 cut(s) 996
AcyI GRCGYC 1 cut(s) 347
AfaI GTAC 8 cut(s) 624, 768, 1035, 1394, 1472, 1707, 1961, 2105
AfeI AGCGCT 2 cut(s) 637, 1403
AfiI CCNNNNNNNGG 5 cut(s) 69, 70, 117, 399, 1435
AflII CTTAAG 2 cut(s) 1664, 2201
AgeI ACCGGT 1 cut(s) 1051
AjiI CACGTC 1 cut(s) 658
AjnI CCWGG 4 cut(s) 63, 110, 392, 785
Alw21I GWGCWC 3 cut(s) 1334, 1692, 2291
Alw26I GTCTC 4 cut(s) 194, 228, 476, 510
Alw44I GTGCAC 1 cut(s) 1330
AlwI GGATC 8 cut(s) 81, 122, 159, 280, 404, 441, 562, 1986
AlwNI CAGNNNCTG 3 cut(s) 117, 399, 1364
Ama87I CYCGRG 5 cut(s) 191, 216, 473, 498, 1691
Aor51HI AGCGCT 2 cut(s) 637, 1403
ApaLI GTGCAC 1 cut(s) 1330
ApeKI GCWGC 4 cut(s) 144, 633, 638, 1301
ApoI RAATTY 4 cut(s) 1312, 1825, 1853, 2016
ArsI GACNNNNNNTTYG 2 cut(s) 1721, 1753
AsiGI ACCGGT 1 cut(s) 1051
AspS9I GGNCC 5 cut(s) 742, 1637, 1754, 1952, 1973
AsuC2I CCSGG 1 cut(s) 953
AsuHPI GGTGA 9 cut(s) 400, 614, 857, 1041, 1546, 1916, 2203, 2243, 2258
AsuII TTCGAA 1 cut(s) 1944
AvaI CYCGRG 5 cut(s) 191, 216, 473, 498, 1691
AvaII GGWCC 1 cut(s) 1952
BaeGI GKGCMC 2 cut(s) 54, 1334
BanII GRGCYC 3 cut(s) 718, 1692, 2291
BauI CACGAG 1 cut(s) 597
BbsI GAAGAC 2 cut(s) 1254, 1734
Bbv12I GWGCWC 3 cut(s) 1334, 1692, 2291
BbvCI CCTCAGC 1 cut(s) 1550
BbvI GCAGC 4 cut(s) 156, 625, 645, 1313
BccI CCATC 9 cut(s) 314, 655, 1531, 1745, 1808, 2074, 2147, 2262, 2308
BceAI ACGGC 5 cut(s) 107, 120, 389, 777, 1277
BciT130I CCWGG 4 cut(s) 65, 112, 394, 787
BciVI GTATCC 1 cut(s) 2125
BcnI CCSGG 1 cut(s) 953
BcoDI GTCTC 4 cut(s) 194, 228, 476, 510
BfaI CTAG 5 cut(s) 281, 563, 969, 1061, 2025
BfmI CTRYAG 3 cut(s) 1509, 2163, 2229
BfoI RGCGCY 3 cut(s) 12, 639, 1405
BfrI CTTAAG 2 cut(s) 1664, 2201
BfuAI ACCTGC 2 cut(s) 315, 1522
BfuI GTATCC 1 cut(s) 2125
BglI GCCNNNNNGGC 1 cut(s) 741
Bme1390I CCNGG 5 cut(s) 65, 112, 394, 787, 953
Bme18I GGWCC 1 cut(s) 1952
BmeT110I CYCGRG 5 cut(s) 191, 216, 473, 498, 1691
BmgBI CACGTC 1 cut(s) 658
BmgT120I GGNCC 5 cut(s) 742, 1637, 1754, 1952, 1973
BmiI GGNNCC 1 cut(s) 1798
BmrFI CCNGG 5 cut(s) 65, 112, 394, 787, 953
BmrI ACTGGG 1 cut(s) 856
BmsI GCATC 5 cut(s) 133, 415, 656, 995, 1447
BmuI ACTGGG 1 cut(s) 856
BpiI GAAGAC 2 cut(s) 1254, 1734
BplI GAGNNNNNCTC 2 cut(s) 700, 732
Bpu10I CCTNAGC 3 cut(s) 1550, 1624, 1686
Bpu14I TTCGAA 1 cut(s) 1944
BpuEI CTTGAG 3 cut(s) 1226, 2034, 2257
BpuMI CCSGG 1 cut(s) 953
BsaAI YACGTR 1 cut(s) 770
BsaHI GRCGYC 1 cut(s) 347
BsaI GGTCTC 4 cut(s) 194, 228, 476, 510
BsaJI CCNNGG 8 cut(s) 63, 64, 136, 418, 952, 1276, 1815, 2187
BsaWI WCCGGW 1 cut(s) 1051
Bsc4I CCNNNNNNNGG 5 cut(s) 69, 70, 117, 399, 1435
Bse118I RCCGGY 2 cut(s) 11, 1051
Bse1I ACTGG 4 cut(s) 862, 1358, 1455, 2132
Bse3DI GCAATG 5 cut(s) 216, 498, 1786, 1884, 1921
BseBI CCWGG 4 cut(s) 65, 112, 394, 787
BseDI CCNNGG 8 cut(s) 63, 64, 136, 418, 952, 1276, 1815, 2187
BseGI GGATG 4 cut(s) 1093, 1249, 2175, 2300
BseLI CCNNNNNNNGG 5 cut(s) 69, 70, 117, 399, 1435
BseMI GCAATG 5 cut(s) 216, 498, 1786, 1884, 1921
BseNI ACTGG 4 cut(s) 862, 1358, 1455, 2132
BseRI GAGGAG 2 cut(s) 153, 435
BseSI GKGCMC 2 cut(s) 54, 1334
BseX3I CGGCCG 1 cut(s) 1812
BseXI GCAGC 4 cut(s) 156, 625, 645, 1313
BseYI CCCAGC 1 cut(s) 69
BsgI GTGCAG 1 cut(s) 624
Bsh1236I CGCG 9 cut(s) 23, 96, 178, 262, 311, 378, 460, 544, 593
Bsh1285I CGRYCG 1 cut(s) 1815
BshTI ACCGGT 1 cut(s) 1051
BsiEI CGRYCG 1 cut(s) 1815
BsiHKAI GWGCWC 3 cut(s) 1334, 1692, 2291
BsiHKCI CYCGRG 5 cut(s) 191, 216, 473, 498, 1691
BsiSI CCGG 4 cut(s) 12, 952, 1052, 1976
BslFI GGGAC 5 cut(s) 102, 309, 384, 591, 844
BslI CCNNNNNNNGG 5 cut(s) 69, 70, 117, 399, 1435
BsmAI GTCTC 4 cut(s) 194, 228, 476, 510
BsmFI GGGAC 5 cut(s) 102, 309, 384, 591, 844
BsmI GAATGC 1 cut(s) 1460
Bso31I GGTCTC 4 cut(s) 194, 228, 476, 510
BsoBI CYCGRG 5 cut(s) 191, 216, 473, 498, 1691
Bsp119I TTCGAA 1 cut(s) 1944
Bsp1286I GDGCHC 5 cut(s) 54, 718, 1334, 1692, 2291
Bsp1407I TGTACA 1 cut(s) 2103
Bsp19I CCATGG 1 cut(s) 2187
BspFNI CGCG 9 cut(s) 23, 96, 178, 262, 311, 378, 460, 544, 593
BspHI TCATGA 2 cut(s) 919, 2283
BspLI GGNNCC 1 cut(s) 1798
BspMAI CTGCAG 1 cut(s) 2233
BspMI ACCTGC 2 cut(s) 315, 1522
BspPI GGATC 8 cut(s) 81, 122, 159, 280, 404, 441, 562, 1986
BspQI GCTCTTC 1 cut(s) 723
BspT104I TTCGAA 1 cut(s) 1944
BspTI CTTAAG 2 cut(s) 1664, 2201
BspTNI GGTCTC 4 cut(s) 194, 228, 476, 510
BsrDI GCAATG 5 cut(s) 216, 498, 1786, 1884, 1921
BsrFI RCCGGY 2 cut(s) 11, 1051
BsrGI TGTACA 1 cut(s) 2103
BsrI ACTGG 4 cut(s) 862, 1358, 1455, 2132
BssAI RCCGGY 2 cut(s) 11, 1051
BssECI CCNNGG 8 cut(s) 63, 64, 136, 418, 952, 1276, 1815, 2187
BssNI GRCGYC 1 cut(s) 347
BssSI CACGAG 1 cut(s) 597
BssT1I CCWWGG 1 cut(s) 2187
Bst2BI CACGAG 1 cut(s) 597
Bst2UI CCWGG 4 cut(s) 65, 112, 394, 787
Bst4CI ACNGT 5 cut(s) 233, 388, 1327, 2113, 2167
Bst6I CTCTTC 8 cut(s) 242, 524, 723, 1076, 1206, 1434, 1481, 1644
BstACI GRCGYC 1 cut(s) 347
BstAFI CTTAAG 2 cut(s) 1664, 2201
BstAUI TGTACA 1 cut(s) 2103
BstBAI YACGTR 1 cut(s) 770
BstBI TTCGAA 1 cut(s) 1944
BstC8I GCNNGC 7 cut(s) 13, 38, 238, 520, 610, 1306, 2229
BstDSI CCRYGG 1 cut(s) 2187
BstEII GGTNACC 4 cut(s) 131, 388, 413, 2191
BstENI CCTNNNNNAGG 1 cut(s) 1433
BstF5I GGATG 4 cut(s) 1093, 1249, 2175, 2300
BstFNI CGCG 9 cut(s) 23, 96, 178, 262, 311, 378, 460, 544, 593
BstH2I RGCGCY 3 cut(s) 12, 639, 1405
BstMAI GTCTC 4 cut(s) 194, 228, 476, 510
BstMCI CGRYCG 1 cut(s) 1815
BstMWI GCNNNNNNNGC 9 cut(s) 17, 175, 373, 457, 519, 644, 741, 820, 1981
BstNI CCWGG 4 cut(s) 65, 112, 394, 787
BstNSI RCATGY 4 cut(s) 165, 447, 1115, 1308
BstPI GGTNACC 4 cut(s) 131, 388, 413, 2191
BstSCI CCNGG 5 cut(s) 63, 110, 392, 785, 951
BstSFI CTRYAG 3 cut(s) 1509, 2163, 2229
BstSLI GKGCMC 2 cut(s) 54, 1334
BstUI CGCG 9 cut(s) 23, 96, 178, 262, 311, 378, 460, 544, 593
BstV1I GCAGC 4 cut(s) 156, 625, 645, 1313
BstV2I GAAGAC 2 cut(s) 1254, 1734
BstX2I RGATCY 4 cut(s) 114, 151, 396, 433
BstYI RGATCY 4 cut(s) 114, 151, 396, 433
BstZI CGGCCG 1 cut(s) 1812
BsuI GTATCC 1 cut(s) 2125
BtgI CCRYGG 1 cut(s) 2187
BtrI CACGTC 1 cut(s) 658
BtsCI GGATG 4 cut(s) 1093, 1249, 2175, 2300
BtsI GCAGTG 2 cut(s) 433, 520
BtsIMutI CAGTG 7 cut(s) 238, 433, 520, 1020, 1332, 1365, 2172
BveI ACCTGC 2 cut(s) 315, 1522
Cac8I GCNNGC 7 cut(s) 13, 38, 238, 520, 610, 1306, 2229
CaiI CAGNNNCTG 3 cut(s) 117, 399, 1364
CciI TCATGA 2 cut(s) 919, 2283
Cfr10I RCCGGY 2 cut(s) 11, 1051
Cfr13I GGNCC 5 cut(s) 742, 1637, 1754, 1952, 1973
Csp6I GTAC 8 cut(s) 623, 767, 1034, 1393, 1471, 1706, 1960, 2104
CspAI ACCGGT 1 cut(s) 1051
CviQI GTAC 8 cut(s) 623, 767, 1034, 1393, 1471, 1706, 1960, 2104
DraI TTTAAA 1 cut(s) 1122
EaeI YGGCCR 2 cut(s) 108, 1812
EagI CGGCCG 1 cut(s) 1812
Eam1104I CTCTTC 8 cut(s) 242, 524, 723, 1076, 1206, 1434, 1481, 1644
EarI CTCTTC 8 cut(s) 242, 524, 723, 1076, 1206, 1434, 1481, 1644
Ecl136II GAGCTC 2 cut(s) 1690, 2289
EclXI CGGCCG 1 cut(s) 1812
Eco130I CCWWGG 1 cut(s) 2187
Eco24I GRGCYC 3 cut(s) 718, 1692, 2291
Eco31I GGTCTC 4 cut(s) 194, 228, 476, 510
Eco32I GATATC 2 cut(s) 1166, 2129
Eco47I GGWCC 1 cut(s) 1952
Eco47III AGCGCT 2 cut(s) 637, 1403
Eco52I CGGCCG 1 cut(s) 1812
Eco53kI GAGCTC 2 cut(s) 1690, 2289
Eco57I CTGAAG 1 cut(s) 996
Eco88I CYCGRG 5 cut(s) 191, 216, 473, 498, 1691
Eco91I GGTNACC 4 cut(s) 131, 388, 413, 2191
EcoICRI GAGCTC 2 cut(s) 1690, 2289
EcoNI CCTNNNNNAGG 1 cut(s) 1433
EcoO65I GGTNACC 4 cut(s) 131, 388, 413, 2191
EcoRII CCWGG 4 cut(s) 63, 110, 392, 785
EcoRV GATATC 2 cut(s) 1166, 2129
EcoT14I CCWWGG 1 cut(s) 2187
EcoT22I ATGCAT 1 cut(s) 1462
EcoT38I GRGCYC 3 cut(s) 718, 1692, 2291
ErhI CCWWGG 1 cut(s) 2187
FaqI GGGAC 5 cut(s) 102, 309, 384, 591, 844
FauI CCCGC 2 cut(s) 286, 568
FblI GTMKAC 1 cut(s) 1238
FokI GGATG 4 cut(s) 1100, 1256, 2162, 2287
FriOI GRGCYC 3 cut(s) 718, 1692, 2291
FspBI CTAG 5 cut(s) 281, 563, 969, 1061, 2025
GsaI CCCAGC 1 cut(s) 73
HaeII RGCGCY 3 cut(s) 12, 639, 1405
HapII CCGG 4 cut(s) 12, 952, 1052, 1976
Hin1I GRCGYC 1 cut(s) 347
HincII GTYRAC 1 cut(s) 687
HindII GTYRAC 1 cut(s) 687
HindIII AAGCTT 2 cut(s) 1190, 2198
HinfI GANTC 4 cut(s) 923, 1730, 1786, 2242
HpaII CCGG 4 cut(s) 12, 952, 1052, 1976
HphI GGTGA 9 cut(s) 400, 614, 857, 1041, 1546, 1916, 2203, 2243, 2258
Hpy166II GTNNAC 7 cut(s) 655, 687, 773, 848, 1239, 1332, 1720
Hpy188I TCNGA 9 cut(s) 46, 102, 384, 961, 1106, 1366, 1528, 1735, 1845
Hpy8I GTNNAC 7 cut(s) 655, 687, 773, 848, 1239, 1332, 1720
HpyCH4III ACNGT 5 cut(s) 233, 388, 1327, 2113, 2167
HpyCH4IV ACGT 4 cut(s) 347, 657, 769, 808
HpyCH4V TGCA 9 cut(s) 367, 641, 940, 1111, 1117, 1332, 1460, 1779, 2231
HpyF10VI GCNNNNNNNGC 9 cut(s) 17, 175, 373, 457, 519, 644, 741, 820, 1981
HpySE526I ACGT 4 cut(s) 347, 657, 769, 808
Hsp92I GRCGYC 1 cut(s) 347
KroI GCCGGC 1 cut(s) 11
KroNI GCCGGC 1 cut(s) 13
LguI GCTCTTC 1 cut(s) 723
LmnI GCTCC 2 cut(s) 121, 403
Lsp1109I GCAGC 4 cut(s) 156, 625, 645, 1313
LweI GCATC 5 cut(s) 133, 415, 656, 995, 1447
MaeI CTAG 5 cut(s) 281, 563, 969, 1061, 2025
MaeII ACGT 4 cut(s) 347, 657, 769, 808
MaeIII GTNAC 5 cut(s) 131, 388, 413, 1904, 2191
MflI RGATCY 4 cut(s) 114, 151, 396, 433
MhlI GDGCHC 5 cut(s) 54, 718, 1334, 1692, 2291
MluCI AATT 9 cut(s) 750, 1312, 1516, 1559, 1825, 1853, 1860, 2016, 2034
MlyI GAGTC 1 cut(s) 1724
MmeI TCCRAC 2 cut(s) 24, 1346
Mph1103I ATGCAT 1 cut(s) 1462
MroNI GCCGGC 1 cut(s) 11
MseI TTAA 5 cut(s) 1121, 1283, 1385, 1665, 2202
MslI CAYNNNNRTG 3 cut(s) 897, 2180, 2274
MspCI CTTAAG 2 cut(s) 1664, 2201
MspI CCGG 4 cut(s) 12, 952, 1052, 1976
MspR9I CCNGG 5 cut(s) 65, 112, 394, 787, 953
Mva1269I GAATGC 1 cut(s) 1460
MvaI CCWGG 4 cut(s) 65, 112, 394, 787
MvnI CGCG 9 cut(s) 23, 96, 178, 262, 311, 378, 460, 544, 593
MwoI GCNNNNNNNGC 9 cut(s) 17, 175, 373, 457, 519, 644, 741, 820, 1981
NaeI GCCGGC 1 cut(s) 13
NciI CCSGG 1 cut(s) 953
NcoI CCATGG 1 cut(s) 2187
NgoMIV GCCGGC 1 cut(s) 11
NlaIV GGNNCC 1 cut(s) 1798
NmeAIII GCCGAG 3 cut(s) 962, 1301, 1840
NmuCI GTSAC 3 cut(s) 388, 1904, 2191
NsiI ATGCAT 1 cut(s) 1462
NspI RCATGY 4 cut(s) 165, 447, 1115, 1308
NspV TTCGAA 1 cut(s) 1944
PaeI GCATGC 1 cut(s) 1308
PaeR7I CTCGAG 3 cut(s) 216, 498, 1691
PagI TCATGA 2 cut(s) 919, 2283
PaqCI CACCTGC 1 cut(s) 1522
PasI CCCWGGG 1 cut(s) 64
PciSI GCTCTTC 1 cut(s) 723
PctI GAATGC 1 cut(s) 1460
PdiI GCCGGC 1 cut(s) 13
PfeI GAWTC 3 cut(s) 923, 1786, 2242
PflMI CCANNNNNTGG 2 cut(s) 117, 399
PfoI TCCNGGA 1 cut(s) 785
PinAI ACCGGT 1 cut(s) 1051
PleI GAGTC 1 cut(s) 1724
PpsI GAGTC 1 cut(s) 1724
Ppu21I YACGTR 1 cut(s) 770
Psp124BI GAGCTC 2 cut(s) 1692, 2291
Psp6I CCWGG 4 cut(s) 63, 110, 392, 785
PspEI GGTNACC 4 cut(s) 131, 388, 413, 2191
PspFI CCCAGC 1 cut(s) 69
PspGI CCWGG 4 cut(s) 63, 110, 392, 785
PspN4I GGNNCC 1 cut(s) 1798
PspPI GGNCC 5 cut(s) 742, 1637, 1754, 1952, 1973
PspXI VCTCGAGB 3 cut(s) 216, 498, 1691
PstI CTGCAG 1 cut(s) 2233
PstNI CAGNNNCTG 3 cut(s) 117, 399, 1364
PsuI RGATCY 4 cut(s) 114, 151, 396, 433
RsaI GTAC 8 cut(s) 624, 768, 1035, 1394, 1472, 1707, 1961, 2105
RsaNI GTAC 8 cut(s) 623, 767, 1034, 1393, 1471, 1706, 1960, 2104
RseI CAYNNNNRTG 3 cut(s) 897, 2180, 2274
SacI GAGCTC 2 cut(s) 1692, 2291
SapI GCTCTTC 1 cut(s) 723
SaqAI TTAA 5 cut(s) 1121, 1283, 1385, 1665, 2202
Sau96I GGNCC 5 cut(s) 742, 1637, 1754, 1952, 1973
SchI GAGTC 1 cut(s) 1724
ScrFI CCNGG 5 cut(s) 65, 112, 394, 787, 953
SduI GDGCHC 5 cut(s) 54, 718, 1334, 1692, 2291
SfaNI GCATC 5 cut(s) 133, 415, 656, 995, 1447
SfcI CTRYAG 3 cut(s) 1509, 2163, 2229
SfiI GGCCNNNNNGGCC 1 cut(s) 741
Sfr274I CTCGAG 3 cut(s) 216, 498, 1691
SfuI TTCGAA 1 cut(s) 1944
SinI GGWCC 1 cut(s) 1952
SlaI CTCGAG 3 cut(s) 216, 498, 1691
SmiI ATTTAAAT 1 cut(s) 1122
SmiMI CAYNNNNRTG 3 cut(s) 897, 2180, 2274
SmlI CTYRAG 8 cut(s) 216, 498, 1205, 1664, 1691, 2049, 2201, 2236
SmoI CTYRAG 8 cut(s) 216, 498, 1205, 1664, 1691, 2049, 2201, 2236
SphI GCATGC 1 cut(s) 1308
Sse9I AATT 9 cut(s) 750, 1312, 1516, 1559, 1825, 1853, 1860, 2016, 2034
SspMI CTAG 5 cut(s) 281, 563, 969, 1061, 2025
SstI GAGCTC 2 cut(s) 1692, 2291
StyD4I CCNGG 5 cut(s) 63, 110, 392, 785, 951
StyI CCWWGG 1 cut(s) 2187
SwaI ATTTAAAT 1 cut(s) 1122
TaaI ACNGT 5 cut(s) 233, 388, 1327, 2113, 2167
TaiI ACGT 4 cut(s) 350, 660, 772, 811
TaqI TCGA 8 cut(s) 217, 275, 499, 557, 1464, 1522, 1692, 1944
TaqII GACCGA 1 cut(s) 1727
TasI AATT 9 cut(s) 750, 1312, 1516, 1559, 1825, 1853, 1860, 2016, 2034
TatI WGTACW 4 cut(s) 622, 1033, 1959, 2103
TfiI GAWTC 3 cut(s) 923, 1786, 2242
Tru1I TTAA 5 cut(s) 1121, 1283, 1385, 1665, 2202
Tru9I TTAA 5 cut(s) 1121, 1283, 1385, 1665, 2202
TscAI CASTG 7 cut(s) 238, 433, 520, 1020, 1332, 1365, 2172
TseFI GTSAC 3 cut(s) 388, 1904, 2191
TseI GCWGC 4 cut(s) 144, 633, 638, 1301
Tsp45I GTSAC 3 cut(s) 388, 1904, 2191
TspDTI ATGAA 8 cut(s) 936, 945, 1172, 1260, 1610, 1796, 1932, 2272
TspGWI ACGGA 2 cut(s) 1256, 1403
TspRI CASTG 7 cut(s) 238, 433, 520, 1020, 1332, 1365, 2172
Van91I CCANNNNNTGG 2 cut(s) 117, 399
Vha464I CTTAAG 2 cut(s) 1664, 2201
VneI GTGCAC 1 cut(s) 1330
VpaK11BI GGWCC 1 cut(s) 1952
XagI CCTNNNNNAGG 1 cut(s) 1433
XapI RAATTY 4 cut(s) 1312, 1825, 1853, 2016
XbaI TCTAGA 1 cut(s) 1060
XceI RCATGY 4 cut(s) 165, 447, 1115, 1308
XhoI CTCGAG 3 cut(s) 216, 498, 1691
XmiI GTMKAC 1 cut(s) 1238
XspI CTAG 5 cut(s) 281, 563, 969, 1061, 2025
ZraI GACGTC 1 cut(s) 348
Zsp2I ATGCAT 1 cut(s) 1462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.