Rh3BG290000

At5g01610-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000560
Physical Location & Seq
Reverse (-)
29055126 .. 29064630
9505 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG290000.1

Sequence Viewer

Length: 735 bp
ATGGAAGAACGCATTCGCCAACGAGAACTTGAATATGCTACGGAGCTGGGCAAGGGAGATTCTGATTTTGAGTTAAAAGAAAAACTAGCAAACATGGACATAGACTGCAATGACACTCATCAACAAACCAGTTCTCTTGTGTGCGATGGAAAAGCCAACCAAGTACCGGCTGATGGAAAATTCCATATTGAAGATGAGCAGCCAATGATTGAGGTAGAAGAAGAACCAGAGTTTGAGAAGCCAAATGATGTTAGCTTTAAAGAAGACTTCATTATCCATGATGAAGACCTCACTGGCCGCGGCGGAAGCTTCAATAGCCGCGACCATGGTGAAGACCTCACCGGCCACTGTAAAGGAGGTGAGGTGGATGAAGGAGTTGTAGCGTCATCATCGCTGAATCAGGTAATGACATTGTCAGCTGACGGAGGAGAGGCCGCAGTAAGCATGGTTTATGTTCTTCCCATGGATTTTTTAGCGCAACCTGACCAGCCACAGTCAATGGAAGGGGATGTTGAGGATGCAGTTTTACCAAAAGTTCTTATTGAAGAGCAATCTCATGCTCAGGATCAGATTCTGAACAAGGCGGGGTCCTACTGGTTCAGCCAGAAGGCCACCAAGGAGATCTCCTCCAGTGATGACATCAACGGAATTTATGATAATATGAACTTGGGATTTTACCTTTTTGCATATGATGACATTGGGCATTTCCTGCCAAAAGGTCTGCAACTCATCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

244

Amino Acids

27.15

Weight (kDa)

4.22

Isoelectric Point (pI)

46.95

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000560)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g21400 FvH4_1g29793 FvH4_2g35581 FvH4_4g18554 FvH4_6g21671
pyrus_communis pycom11g14070
rosa_chinensis RchiOBHm_Chr6g0263201
rosa_laevigata RLG00000001861 RLG00000002050 RLG00000018783 RLG00000022649 RLG00000030152
rosa_multiflora Rmu_co8107100.1_g000001 Rmu_sc0000805.1_g000009 Rmu_sc0001347.1_g000002 Rmu_sc0001896.1_g000004 Rmu_sc0002170.1_g000040 Rmu_sc0002413.1_g000005 Rmu_sc0002489.1_g000053 Rmu_sc0002942.1_g000015 Rmu_sc0003071.1_g000022 Rmu_sc0003623.1_g000021 Rmu_sc0004063.1_g000005 Rmu_sc0004574.1_g000014 Rmu_sc0004723.1_g000009 Rmu_sc0005177.1_g000013 Rmu_sc0005994.1_g000011 Rmu_sc0006163.1_g000006 Rmu_sc0006583.1_g000008 Rmu_sc0008303.1_g000005 Rmu_sc0009027.1_g000002 Rmu_sc0009027.1_g000003 Rmu_sc0011790.1_g000009 Rmu_sc0011833.1_g000005 Rmu_sc0013493.1_g000006 Rmu_sc0016701.1_g000002 Rmu_sc0022033.1_g000001 Rmu_sc0022185.1_g000001 Rmu_sc0027245.1_g000005 Rmu_sc0030292.1_g000002 Rmu_ssc0000242.1_g000003
rosa_roxburghii Rroxscaffold_1G00015200 Rroxscaffold_1G00059290 Rroxscaffold_2G00079850 Rroxscaffold_2G00079860 Rroxscaffold_2G00107520 Rroxscaffold_2G00123420 Rroxscaffold_2G00123430 Rroxscaffold_3G00265790 Rroxscaffold_5G00335960 Rroxscaffold_5G00338270 Rroxscaffold_5G00343980 Rroxscaffold_5G00343990
rosa_samantha Rh1AG036500 Rh1DG429900 Rh2AG172600 Rh2AG610900 Rh2BG180200 Rh2BG180300 Rh2BG340600 Rh2BG400300 Rh2BG468400 Rh2BG478200 Rh2BG612500 Rh2BG612600 Rh3BG290000 Rh3BG290100 Rh3BG345200 Rh3BG345300 Rh3BG359800 Rh3CG291300 Rh4CG156300 Rh4CG264600 Rh5AG430300 Rh5BG185400 Rh6DG198700 Rh7CG199900 Rh7CG200000 Rh7CG370300 Rh7CG370600 Rh7CG395500 Rh7DG405800 Rh7DG405900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 300, 321
AciI CCGC 6 cut(s) 298, 300, 303, 319, 435, 584
AclWI GGATC 1 cut(s) 573
AcoI YGGCCR 2 cut(s) 295, 343
AcsI RAATTY 2 cut(s) 179, 648
AfaI GTAC 1 cut(s) 165
AfiI CCNNNNNNNGG 2 cut(s) 166, 173
AgsI TTSAA 4 cut(s) 32, 191, 313, 545
AluBI AGCT 4 cut(s) 46, 255, 309, 419
AluI AGCT 4 cut(s) 46, 255, 309, 419
AlwI GGATC 1 cut(s) 573
AlwNI CAGNNNCTG 1 cut(s) 574
AoxI GGCC 4 cut(s) 295, 343, 432, 609
ApeKI GCWGC 1 cut(s) 199
ApoI RAATTY 2 cut(s) 179, 648
Asp700I GAANNNNTTC 1 cut(s) 12
AspLEI GCGC 1 cut(s) 478
AspS9I GGNCC 1 cut(s) 588
AsuHPI GGTGA 3 cut(s) 331, 341, 371
AvaII GGWCC 1 cut(s) 588
BbsI GAAGAC 3 cut(s) 270, 291, 339
BbvI GCAGC 1 cut(s) 211
BccI CCATC 2 cut(s) 140, 167
BfaI CTAG 2 cut(s) 86, 733
BglII AGATCT 1 cut(s) 621
BisI GCNGC 5 cut(s) 200, 298, 301, 319, 435
BlsI GCNGC 5 cut(s) 201, 299, 302, 320, 436
Bme18I GGWCC 1 cut(s) 588
BmgT120I GGNCC 1 cut(s) 588
BmiI GGNNCC 1 cut(s) 589
BmsI GCATC 1 cut(s) 508
BpiI GAAGAC 3 cut(s) 270, 291, 339
BplI GAGNNNNNCTC 2 cut(s) 611, 643
BpmI CTGGAG 1 cut(s) 613
Bpu10I CCTNAGC 1 cut(s) 561
BsaJI CCNNGG 4 cut(s) 298, 325, 462, 615
BsaXI ACNNNNNCTCC 2 cut(s) 348, 378
Bsc4I CCNNNNNNNGG 2 cut(s) 166, 173
Bse118I RCCGGY 2 cut(s) 166, 341
Bse1I ACTGG 4 cut(s) 129, 298, 599, 630
Bse3DI GCAATG 1 cut(s) 115
BseDI CCNNGG 4 cut(s) 298, 325, 462, 615
BseGI GGATG 3 cut(s) 373, 514, 523
BseLI CCNNNNNNNGG 2 cut(s) 166, 173
BseMI GCAATG 1 cut(s) 115
BseMII CTCAG 1 cut(s) 575
BseNI ACTGG 4 cut(s) 129, 298, 599, 630
BseRI GAGGAG 2 cut(s) 441, 616
BseXI GCAGC 1 cut(s) 211
BseYI CCCAGC 1 cut(s) 46
Bsh1236I CGCG 2 cut(s) 300, 321
BshFI GGCC 4 cut(s) 297, 345, 434, 611
BsiSI CCGG 2 cut(s) 167, 342
BslI CCNNNNNNNGG 2 cut(s) 166, 173
BsmI GAATGC 1 cut(s) 12
BsnI GGCC 4 cut(s) 297, 345, 434, 611
Bsp143I GATC 2 cut(s) 565, 621
Bsp19I CCATGG 2 cut(s) 325, 462
BspACI CCGC 6 cut(s) 298, 300, 303, 319, 435, 584
BspANI GGCC 4 cut(s) 297, 345, 434, 611
BspCNI CTCAG 1 cut(s) 574
BspFNI CGCG 2 cut(s) 300, 321
BspLI GGNNCC 1 cut(s) 589
BspPI GGATC 1 cut(s) 573
BspQI GCTCTTC 1 cut(s) 540
BsrDI GCAATG 1 cut(s) 115
BsrFI RCCGGY 2 cut(s) 166, 341
BsrI ACTGG 4 cut(s) 129, 298, 599, 630
BssAI RCCGGY 2 cut(s) 166, 341
BssECI CCNNGG 4 cut(s) 298, 325, 462, 615
BssMI GATC 2 cut(s) 565, 621
BssT1I CCWWGG 3 cut(s) 325, 462, 615
Bst4CI ACNGT 2 cut(s) 350, 495
Bst6I CTCTTC 1 cut(s) 540
BstAPI GCANNNNNTGC 1 cut(s) 709
BstDEI CTNAG 1 cut(s) 561
BstDSI CCRYGG 3 cut(s) 298, 325, 462
BstF5I GGATG 3 cut(s) 373, 514, 523
BstFNI CGCG 2 cut(s) 300, 321
BstHHI GCGC 1 cut(s) 478
BstKTI GATC 2 cut(s) 568, 624
BstMBI GATC 2 cut(s) 565, 621
BstMWI GCNNNNNNNGC 3 cut(s) 306, 315, 709
BstUI CGCG 2 cut(s) 300, 321
BstV1I GCAGC 1 cut(s) 211
BstV2I GAAGAC 3 cut(s) 270, 291, 339
BstX2I RGATCY 1 cut(s) 621
BstYI RGATCY 1 cut(s) 621
BsuRI GGCC 4 cut(s) 297, 345, 434, 611
BtgI CCRYGG 3 cut(s) 298, 325, 462
BtgZI GCGATG 2 cut(s) 159, 375
BtsCI GGATG 3 cut(s) 373, 514, 523
BtsIMutI CAGTG 3 cut(s) 291, 346, 637
CaiI CAGNNNCTG 1 cut(s) 574
CfoI GCGC 1 cut(s) 478
Cfr10I RCCGGY 2 cut(s) 166, 341
Cfr13I GGNCC 1 cut(s) 588
Cfr42I CCGCGG 1 cut(s) 301
CseI GACGC 1 cut(s) 372
Csp6I GTAC 1 cut(s) 164
CviAII CATG 6 cut(s) 94, 278, 326, 445, 463, 557
CviQI GTAC 1 cut(s) 164
DdeI CTNAG 1 cut(s) 561
DpnI GATC 2 cut(s) 567, 623
DpnII GATC 2 cut(s) 565, 621
DraI TTTAAA 1 cut(s) 259
EaeI YGGCCR 2 cut(s) 295, 343
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
EciI GGCGGA 1 cut(s) 318
Eco130I CCWWGG 3 cut(s) 325, 462, 615
Eco47I GGWCC 1 cut(s) 588
EcoO109I RGGNCCY 1 cut(s) 588
EcoT14I CCWWGG 3 cut(s) 325, 462, 615
ErhI CCWWGG 3 cut(s) 325, 462, 615
FaeI CATG 6 cut(s) 97, 281, 329, 448, 466, 560
FatI CATG 6 cut(s) 93, 277, 325, 444, 462, 556
FauI CCCGC 1 cut(s) 577
FauNDI CATATG 1 cut(s) 688
Fnu4HI GCNGC 5 cut(s) 200, 298, 301, 319, 435
FokI GGATG 3 cut(s) 380, 521, 530
Fsp4HI GCNGC 5 cut(s) 200, 298, 301, 319, 435
FspBI CTAG 2 cut(s) 86, 733
GlaI GCGC 1 cut(s) 477
GluI GCNGC 5 cut(s) 200, 298, 301, 319, 435
GsaI CCCAGC 1 cut(s) 50
GsuI CTGGAG 1 cut(s) 613
HaeIII GGCC 4 cut(s) 297, 345, 434, 611
HapII CCGG 2 cut(s) 167, 342
HgaI GACGC 1 cut(s) 372
HhaI GCGC 1 cut(s) 478
Hin1II CATG 6 cut(s) 97, 281, 329, 448, 466, 560
Hin6I GCGC 1 cut(s) 476
HinP1I GCGC 1 cut(s) 476
HindIII AAGCTT 1 cut(s) 307
HinfI GANTC 3 cut(s) 59, 397, 571
HpaII CCGG 2 cut(s) 167, 342
HphI GGTGA 3 cut(s) 331, 341, 371
Hpy188I TCNGA 3 cut(s) 64, 570, 576
Hpy188III TCNNGA 1 cut(s) 563
HpyAV CCTTC 3 cut(s) 365, 497, 601
HpyCH4III ACNGT 2 cut(s) 350, 495
HpyCH4V TGCA 4 cut(s) 108, 521, 686, 724
HpyF10VI GCNNNNNNNGC 3 cut(s) 306, 315, 709
HpyF3I CTNAG 1 cut(s) 561
Hsp92II CATG 6 cut(s) 97, 281, 329, 448, 466, 560
HspAI GCGC 1 cut(s) 476
KspI CCGCGG 1 cut(s) 301
Kzo9I GATC 2 cut(s) 565, 621
LguI GCTCTTC 1 cut(s) 540
LmnI GCTCC 1 cut(s) 43
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 1 cut(s) 508
MaeI CTAG 2 cut(s) 86, 733
MalI GATC 2 cut(s) 567, 623
MboI GATC 2 cut(s) 565, 621
MboII GAAGA 9 cut(s) 17, 203, 230, 233, 275, 296, 344, 449, 557
MflI RGATCY 1 cut(s) 621
MluCI AATT 2 cut(s) 179, 648
MnlI CCTC 9 cut(s) 205, 299, 347, 350, 355, 419, 424, 508, 637
MroXI GAANNNNTTC 1 cut(s) 12
MseI TTAA 2 cut(s) 74, 258
MspA1I CMGCKG 2 cut(s) 300, 419
MspI CCGG 2 cut(s) 167, 342
Mva1269I GAATGC 1 cut(s) 12
MvnI CGCG 2 cut(s) 300, 321
MwoI GCNNNNNNNGC 3 cut(s) 306, 315, 709
NcoI CCATGG 2 cut(s) 325, 462
NdeI CATATG 1 cut(s) 688
NdeII GATC 2 cut(s) 565, 621
NlaIII CATG 6 cut(s) 97, 281, 329, 448, 466, 560
NlaIV GGNNCC 1 cut(s) 589
PciSI GCTCTTC 1 cut(s) 540
PctI GAATGC 1 cut(s) 12
PdmI GAANNNNTTC 1 cut(s) 12
PfeI GAWTC 3 cut(s) 59, 397, 571
PflFI GACNNNGTC 1 cut(s) 412
PkrI GCNGC 5 cut(s) 201, 299, 302, 320, 436
PpuMI RGGWCCY 1 cut(s) 588
Psp5II RGGWCCY 1 cut(s) 588
PspFI CCCAGC 1 cut(s) 46
PspN4I GGNNCC 1 cut(s) 589
PspPI GGNCC 1 cut(s) 588
PspPPI RGGWCCY 1 cut(s) 588
PstNI CAGNNNCTG 1 cut(s) 574
PsuI RGATCY 1 cut(s) 621
PsyI GACNNNGTC 1 cut(s) 412
PvuII CAGCTG 1 cut(s) 419
RsaI GTAC 1 cut(s) 165
RsaNI GTAC 1 cut(s) 164
SacII CCGCGG 1 cut(s) 301
SapI GCTCTTC 1 cut(s) 540
SaqAI TTAA 2 cut(s) 74, 258
SatI GCNGC 5 cut(s) 200, 298, 301, 319, 435
Sau3AI GATC 2 cut(s) 565, 621
Sau96I GGNCC 1 cut(s) 588
SfaNI GCATC 1 cut(s) 508
Sfr303I CCGCGG 1 cut(s) 301
SgrBI CCGCGG 1 cut(s) 301
SinI GGWCC 1 cut(s) 588
Sse9I AATT 2 cut(s) 179, 648
SsiI CCGC 6 cut(s) 298, 300, 303, 319, 435, 584
SspMI CTAG 2 cut(s) 86, 733
StyI CCWWGG 3 cut(s) 325, 462, 615
TaaI ACNGT 2 cut(s) 350, 495
TasI AATT 2 cut(s) 179, 648
TauI GCSGC 4 cut(s) 300, 303, 321, 437
TfiI GAWTC 3 cut(s) 59, 397, 571
Tru1I TTAA 2 cut(s) 74, 258
Tru9I TTAA 2 cut(s) 74, 258
TscAI CASTG 3 cut(s) 298, 353, 637
TseI GCWGC 1 cut(s) 199
TspDTI ATGAA 4 cut(s) 259, 297, 384, 677
TspGWI ACGGA 3 cut(s) 56, 438, 660
TspRI CASTG 3 cut(s) 298, 353, 637
Tth111I GACNNNGTC 1 cut(s) 412
VpaK11BI GGWCC 1 cut(s) 588
XapI RAATTY 2 cut(s) 179, 648
XmnI GAANNNNTTC 1 cut(s) 12
XspI CTAG 2 cut(s) 86, 733
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.