MD12G1213300.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000404
Physical Location & Seq
Forward (+)
29169672 .. 29172842
3171 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1213300.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGAGAGGAGAAGTTGAGGTGCTCCAGAAGTTGATTGATAAGAATCCTGAGTCCATTTATGTCAAAGTTGAAAACGGATCGAAAGAAACAGTTTTGCACTTGTGTATTAAACACAACCAGTTAGAGTGCTTGAAAGTGTTGATTACAAGAGACGACCACAATGGTGAGTTCCTCAACTCAAGAGCCGGCTGTGATGGTGGTGTGACCATCCTGCACTTAGCATTGATGCTAAGGCAAATTAAGACTATACGTTACCTGCTTTCCGTTGATGCTATAAGAGCAGAAGCAATTGGAATGAATGGGATGTCTCTGACCATGTTAGATATCTTAGAGTACAGCAGCATTGCAAGAGAGGACTTTAGCAGAAGCTTAGAAATTCAACAGATTCTGATAGACGCCGGATTAATCAGAAGAGAAAATGATGAAAATGATAAGCCTAACTCAGATGTTGACGCTGCTGCTGTTGTATCACCAAATCCAAGAAGGGTAAAGAAGAAAGAGAAGCTTCACAAACAAGGAGCATCATCGGAAAAGGGATCAAAGCCAGCAAGGCGGTGGGGGATAAAATTGATGAAATGGTTGAGATATCCGAGTGATTGGTTGGTCGAGACACGTGGCATGCTGATGGTTGTGGCTACGATGATCTCGACCATGACTTTTCAAGCCGTAGTTAACCCACCTGGTGGTGTTTGGCAAAATGATGTTACCAACTCTACCGAAGCTGGTTGCACTGCAGAGAATATATGTACTGCTGGAACTGCAGTGTTAGGCTACGTTTGGGAAGACGACTTCGTCACTTTCATAAAATACAATACCACCTCGTTCCTTGCTTCTTTGTGTGTCACACTTTTGCTCATTAGTGGATTTCCTCTCCACAATCGGTTGTGCATGTGGCTCTTATCGATGTCCATGTGCGTCACCCTTACATTCCTGGCACTCACCTACCTACAAGTGCTGCTCTTGATCATCCCTGTTAACGGCATTTTCGATTCATATAGAATCATTTGCAAGAATTGCTTTTATACTTGGGTTGCGTTGCTGGTCATAGTCGGTGTACTTCACACTATTCGGTTTCTTATTTGGGTGGTGAAGAGGTTGCGGCCTCAATACTTCAGGACAAAATCAGTTCTCAAGAACATGATCGCCACTGGCTCCTTTTCATGTAACGATCCAATGCGTTTTCAAGAGATTGATTTACAACCAGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

403

Amino Acids

45.45

Weight (kDa)

8.68

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 3 - 88 9.5e-10 Ankyrin repeats (3 copies)
PGG PF13962 199 - 317 3.6e-19 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13950 AT4G13266
fragaria_vesca FvH4_7g06240 FvH4_7g06250 FvH4_7g06270
malus_domestica MD12G1213300.v1.1 MD12G1213900.v1.1 MD12G1214100.v1.1 MD12G1214700.v1.1 MD12G1214800.v1.1
prunus_persica Prupe.2G107700_v2.0.a1 Prupe.3G142200_v2.0.a1 Prupe.3G154100_v2.0.a1 Prupe.3G154300_v2.0.a1 Prupe.3G154900_v2.0.a1 Prupe.3G155100_v2.0.a1
pyrus_communis pycom12g20010 pycom12g20080 pycom12g20090 pycom12g20100
rosa_chinensis RchiOBHm_Chr1g0341001 RchiOBHm_Chr1g0341021 RchiOBHm_Chr1g0341891 RchiOBHm_Chr1g0342341 RchiOBHm_Chr3g0490431
rosa_laevigata RLG00000022889 RLG00000029016 RLG00000029039 RLG00000029059 RLG00000029061 RLG00000029065 RLG00000029115 RLG00000029155 RLG00000033311 RLG00000033312
rosa_multiflora Rmu_sc0000404.1_g000006 Rmu_sc0001836.1_g000015 Rmu_sc0001836.1_g000050 Rmu_sc0002200.1_g000003 Rmu_sc0003862.1_g000020 Rmu_sc0005193.1_g000001 Rmu_sc0005193.1_g000002 Rmu_sc0008453.1_g000008 Rmu_sc0008453.1_g000009 Rmu_sc0008453.1_g000010 Rmu_sc0008862.1_g000001 Rmu_sc0011195.1_g000003 Rmu_sc0014077.1_g000001
rosa_roxburghii Rroxscaffold_4G00311670 Rroxscaffold_4G00311730 Rroxscaffold_4G00311750 Rroxscaffold_4G00312290 Rroxscaffold_6G00393430
rosa_rugosa Rorug01G0153800.1 Rorug01G0153900.1 Rorug01G0160100.1 Rorug03G0243800
rosa_samantha Rh1AG166400 Rh1AG168200 Rh1AG174100 Rh1AG174700 Rh1AG179000 Rh1BG132600 Rh1BG136500 Rh1BG142200 Rh1BG142800 Rh1BG143200 Rh1BG146200 Rh1BG147000 Rh1BG147100 Rh1CG154800 Rh1CG161900 Rh1CG162700 Rh1CG166000 Rh1DG167500 Rh1DG169900 Rh1DG174000 Rh1DG174400 Rh1DG174700 Rh1DG174800 Rh1DG178200 Rh1DG179000 Rh1DG179100 Rh3AG292800 Rh3BG329600 Rh3CG326900 Rh3DG326200 Rh5BG218300 Rh7CG326800 Rh7DG307100
rosa_wichuraiana Rw0G006190 Rw0G007830 Rw0G009970 Rw0G009980 Rw1G013880 Rw1G014000 Rw1G014500 Rw1G014560 Rw1G014920 Rw3G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 791
Acc36I ACCTGC 1 cut(s) 264
AccB7I CCANNNNNTGG 1 cut(s) 683
AciI CCGC 2 cut(s) 553, 1099
AclWI GGATC 3 cut(s) 85, 544, 1163
AcsI RAATTY 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 1096
AcvI CACGTG 1 cut(s) 614
AcyI GRCGYC 1 cut(s) 396
AdeI CACNNNGTG 1 cut(s) 683
AfaI GTAC 3 cut(s) 335, 748, 1056
AfiI CCNNNNNNNGG 2 cut(s) 683, 977
AflIII ACRYGT 1 cut(s) 611
AgsI TTSAA 5 cut(s) 71, 133, 380, 662, 1184
AjnI CCWGG 2 cut(s) 679, 930
AleI CACNNNNGTG 1 cut(s) 162
AluBI AGCT 3 cut(s) 369, 505, 722
AluI AGCT 3 cut(s) 369, 505, 722
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 3 cut(s) 144, 312, 602
AlwI GGATC 3 cut(s) 85, 544, 1163
AlwNI CAGNNNCTG 1 cut(s) 388
AoxI GGCC 1 cut(s) 1100
ApeKI GCWGC 4 cut(s) 339, 455, 458, 955
ApoI RAATTY 1 cut(s) 375
AseI ATTAAT 1 cut(s) 404
AsuHPI GGTGA 5 cut(s) 176, 462, 910, 931, 1099
BbrPI CACGTG 1 cut(s) 614
BbsI GAAGAC 1 cut(s) 789
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 4 cut(s) 351, 442, 445, 942
BccI CCATC 3 cut(s) 188, 215, 619
BceAI ACGGC 2 cut(s) 650, 994
BciT130I CCWGG 2 cut(s) 681, 932
BclI TGATCA 1 cut(s) 963
BcoDI GTCTC 3 cut(s) 144, 312, 602
BfmI CTRYAG 2 cut(s) 732, 759
BfuAI ACCTGC 1 cut(s) 264
BglI GCCNNNNNGGC 1 cut(s) 550
BisI GCNGC 5 cut(s) 340, 456, 459, 956, 1100
BlsI GCNGC 5 cut(s) 341, 457, 460, 957, 1101
Bme1390I CCNGG 2 cut(s) 681, 932
BmiI GGNNCC 1 cut(s) 1153
BmrFI CCNGG 2 cut(s) 681, 932
BmsI GCATC 3 cut(s) 216, 259, 530
BpiI GAAGAC 1 cut(s) 789
BpmI CTGGAG 1 cut(s) 8
Bpu10I CCTNAGC 1 cut(s) 230
BpuEI CTTGAG 2 cut(s) 163, 1115
Bsa29I ATCGAT 1 cut(s) 902
BsaAI YACGTR 1 cut(s) 614
BsaBI GATNNNNATC 1 cut(s) 42
BsaHI GRCGYC 1 cut(s) 396
Bsc4I CCNNNNNNNGG 2 cut(s) 683, 977
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 3 cut(s) 118, 1153, 1202
Bse3DI GCAATG 1 cut(s) 342
Bse8I GATNNNNATC 1 cut(s) 42
BseBI CCWGG 2 cut(s) 681, 932
BseCI ATCGAT 1 cut(s) 902
BseGI GGATG 3 cut(s) 207, 309, 966
BseJI GATNNNNATC 1 cut(s) 42
BseLI CCNNNNNNNGG 2 cut(s) 683, 977
BseMI GCAATG 1 cut(s) 342
BseMII CTCAG 2 cut(s) 39, 456
BseNI ACTGG 3 cut(s) 118, 1153, 1202
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 4 cut(s) 351, 442, 445, 942
BsgI GTGCAG 1 cut(s) 197
BshFI GGCC 1 cut(s) 1102
BshVI ATCGAT 1 cut(s) 902
BsiHKAI GWGCWC 1 cut(s) 24
BsiSI CCGG 2 cut(s) 186, 399
BslI CCNNNNNNNGG 2 cut(s) 683, 977
BsmAI GTCTC 3 cut(s) 144, 312, 602
BsmBI CGTCTC 1 cut(s) 144
BsnI GGCC 1 cut(s) 1102
Bsp1286I GDGCHC 1 cut(s) 24
Bsp143I GATC 6 cut(s) 77, 536, 642, 963, 1140, 1168
BspACI CCGC 2 cut(s) 553, 1099
BspANI GGCC 1 cut(s) 1102
BspCNI CTCAG 2 cut(s) 40, 455
BspDI ATCGAT 1 cut(s) 902
BspLI GGNNCC 1 cut(s) 1153
BspMAI CTGCAG 2 cut(s) 736, 763
BspMI ACCTGC 1 cut(s) 264
BspPI GGATC 3 cut(s) 85, 544, 1163
BsrDI GCAATG 1 cut(s) 342
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 3 cut(s) 118, 1153, 1202
BssAI RCCGGY 1 cut(s) 185
BssMI GATC 6 cut(s) 77, 536, 642, 963, 1140, 1168
BssNI GRCGYC 1 cut(s) 396
Bst2UI CCWGG 2 cut(s) 681, 932
Bst4CI ACNGT 1 cut(s) 91
Bst6I CTCTTC 2 cut(s) 406, 1085
BstACI GRCGYC 1 cut(s) 396
BstAPI GCANNNNNTGC 1 cut(s) 1014
BstBAI YACGTR 1 cut(s) 614
BstC8I GCNNGC 3 cut(s) 187, 546, 620
BstDEI CTNAG 6 cut(s) 48, 217, 230, 328, 370, 442
BstF5I GGATG 3 cut(s) 207, 309, 966
BstKTI GATC 6 cut(s) 80, 539, 645, 966, 1143, 1171
BstMAI GTCTC 3 cut(s) 144, 312, 602
BstMBI GATC 6 cut(s) 77, 536, 642, 963, 1140, 1168
BstMWI GCNNNNNNNGC 4 cut(s) 278, 550, 758, 1014
BstNI CCWGG 2 cut(s) 681, 932
BstNSI RCATGY 2 cut(s) 622, 892
BstSCI CCNGG 2 cut(s) 679, 930
BstSFI CTRYAG 2 cut(s) 732, 759
BstV1I GCAGC 4 cut(s) 351, 442, 445, 942
BstV2I GAAGAC 1 cut(s) 789
Bsu15I ATCGAT 1 cut(s) 902
BsuRI GGCC 1 cut(s) 1102
BsuTUI ATCGAT 1 cut(s) 902
BtsCI GGATG 3 cut(s) 207, 309, 966
BtsI GCAGTG 2 cut(s) 729, 768
BtsIMutI CAGTG 4 cut(s) 729, 768, 1146, 1209
BveI ACCTGC 1 cut(s) 264
Cac8I GCNNGC 3 cut(s) 187, 546, 620
CaiI CAGNNNCTG 1 cut(s) 388
Cfr10I RCCGGY 1 cut(s) 185
ClaI ATCGAT 1 cut(s) 902
CseI GACGC 3 cut(s) 404, 461, 904
CsiI ACCWGGT 1 cut(s) 679
Csp6I GTAC 3 cut(s) 334, 747, 1055
CviAII CATG 7 cut(s) 316, 619, 652, 889, 910, 1138, 1161
CviQI GTAC 3 cut(s) 334, 747, 1055
DdeI CTNAG 6 cut(s) 48, 217, 230, 328, 370, 442
DpnI GATC 6 cut(s) 79, 538, 644, 965, 1142, 1170
DpnII GATC 6 cut(s) 77, 536, 642, 963, 1140, 1168
DraIII CACNNNGTG 1 cut(s) 683
DrdI GACNNNNNNGTC 1 cut(s) 791
DseDI GACNNNNNNGTC 1 cut(s) 791
Eam1104I CTCTTC 2 cut(s) 406, 1085
EarI CTCTTC 2 cut(s) 406, 1085
Eco32I GATATC 2 cut(s) 325, 587
Eco57I CTGAAG 1 cut(s) 1096
Eco72I CACGTG 1 cut(s) 614
EcoRII CCWGG 2 cut(s) 679, 930
EcoRV GATATC 2 cut(s) 325, 587
Esp3I CGTCTC 1 cut(s) 144
FaeI CATG 7 cut(s) 319, 622, 655, 892, 913, 1141, 1164
FalI AAGNNNNNCTT 4 cut(s) 489, 521, 1001, 1033
FatI CATG 7 cut(s) 315, 618, 651, 888, 909, 1137, 1160
FbaI TGATCA 1 cut(s) 963
Fnu4HI GCNGC 5 cut(s) 340, 456, 459, 956, 1100
FokI GGATG 3 cut(s) 194, 316, 953
Fsp4HI GCNGC 5 cut(s) 340, 456, 459, 956, 1100
GluI GCNGC 5 cut(s) 340, 456, 459, 956, 1100
GsuI CTGGAG 1 cut(s) 8
HaeIII GGCC 1 cut(s) 1102
HapII CCGG 2 cut(s) 186, 399
HgaI GACGC 3 cut(s) 404, 461, 904
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 7 cut(s) 319, 622, 655, 892, 913, 1141, 1164
HincII GTYRAC 3 cut(s) 451, 673, 976
HindII GTYRAC 3 cut(s) 451, 673, 976
HindIII AAGCTT 2 cut(s) 367, 503
HinfI GANTC 5 cut(s) 43, 50, 385, 989, 999
HpaI GTTAAC 2 cut(s) 673, 976
HpaII CCGG 2 cut(s) 186, 399
HphI GGTGA 5 cut(s) 176, 462, 910, 931, 1099
Hpy166II GTNNAC 4 cut(s) 451, 673, 976, 1055
Hpy188I TCNGA 6 cut(s) 312, 390, 410, 445, 529, 591
Hpy188III TCNNGA 9 cut(s) 25, 47, 180, 607, 646, 961, 1114, 1132, 1184
Hpy8I GTNNAC 4 cut(s) 451, 673, 976, 1055
HpyAV CCTTC 1 cut(s) 477
HpyCH4III ACNGT 1 cut(s) 91
HpyCH4IV ACGT 3 cut(s) 250, 613, 774
HpyCH4V TGCA 8 cut(s) 97, 214, 347, 729, 734, 761, 888, 1008
HpyF10VI GCNNNNNNNGC 4 cut(s) 278, 550, 758, 1014
HpyF3I CTNAG 6 cut(s) 48, 217, 230, 328, 370, 442
HpySE526I ACGT 3 cut(s) 250, 613, 774
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 7 cut(s) 319, 622, 655, 892, 913, 1141, 1164
KroI GCCGGC 1 cut(s) 185
KroNI GCCGGC 1 cut(s) 187
Ksp22I TGATCA 1 cut(s) 963
KspAI GTTAAC 2 cut(s) 673, 976
Kzo9I GATC 6 cut(s) 77, 536, 642, 963, 1140, 1168
LmnI GCTCC 3 cut(s) 27, 518, 1157
Lsp1109I GCAGC 4 cut(s) 351, 442, 445, 942
LweI GCATC 3 cut(s) 216, 259, 530
MabI ACCWGGT 1 cut(s) 679
MaeII ACGT 3 cut(s) 250, 613, 774
MaeIII GTNAC 7 cut(s) 202, 251, 703, 793, 841, 916, 1163
MalI GATC 6 cut(s) 79, 538, 644, 965, 1142, 1170
MboI GATC 6 cut(s) 77, 536, 642, 963, 1140, 1168
MboII GAAGA 4 cut(s) 423, 505, 794, 1102
MfeI CAATTG 1 cut(s) 288
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 5 cut(s) 237, 288, 375, 566, 1012
MlyI GAGTC 1 cut(s) 59
MnlI CCTC 7 cut(s) 10, 182, 346, 829, 879, 1086, 1113
MroNI GCCGGC 1 cut(s) 185
MseI TTAA 5 cut(s) 108, 240, 404, 672, 975
MslI CAYNNNNRTG 2 cut(s) 162, 623
MspI CCGG 2 cut(s) 186, 399
MspR9I CCNGG 2 cut(s) 681, 932
MunI CAATTG 1 cut(s) 288
MvaI CCWGG 2 cut(s) 681, 932
MwoI GCNNNNNNNGC 4 cut(s) 278, 550, 758, 1014
NaeI GCCGGC 1 cut(s) 187
NdeII GATC 6 cut(s) 77, 536, 642, 963, 1140, 1168
NgoMIV GCCGGC 1 cut(s) 185
NlaIII CATG 7 cut(s) 319, 622, 655, 892, 913, 1141, 1164
NlaIV GGNNCC 1 cut(s) 1153
NmuCI GTSAC 4 cut(s) 202, 793, 841, 916
NspI RCATGY 2 cut(s) 622, 892
OliI CACNNNNGTG 1 cut(s) 162
PaeI GCATGC 1 cut(s) 622
PcsI WCGNNNNNNNCGW 2 cut(s) 644, 984
PdiI GCCGGC 1 cut(s) 187
PfeI GAWTC 4 cut(s) 43, 385, 989, 999
PflFI GACNNNGTC 1 cut(s) 791
PflMI CCANNNNNTGG 1 cut(s) 683
PkrI GCNGC 5 cut(s) 341, 457, 460, 957, 1101
PleI GAGTC 1 cut(s) 58
PmaCI CACGTG 1 cut(s) 614
PmlI CACGTG 1 cut(s) 614
PpsI GAGTC 1 cut(s) 58
Ppu21I YACGTR 1 cut(s) 614
PshBI ATTAAT 1 cut(s) 404
Psp6I CCWGG 2 cut(s) 679, 930
PspCI CACGTG 1 cut(s) 614
PspGI CCWGG 2 cut(s) 679, 930
PspN4I GGNNCC 1 cut(s) 1153
PstI CTGCAG 2 cut(s) 736, 763
PstNI CAGNNNCTG 1 cut(s) 388
PsyI GACNNNGTC 1 cut(s) 791
RsaI GTAC 3 cut(s) 335, 748, 1056
RsaNI GTAC 3 cut(s) 334, 747, 1055
RseI CAYNNNNRTG 2 cut(s) 162, 623
SaqAI TTAA 5 cut(s) 108, 240, 404, 672, 975
SatI GCNGC 5 cut(s) 340, 456, 459, 956, 1100
Sau3AI GATC 6 cut(s) 77, 536, 642, 963, 1140, 1168
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 2 cut(s) 681, 932
SduI GDGCHC 1 cut(s) 24
SexAI ACCWGGT 1 cut(s) 679
SfaNI GCATC 3 cut(s) 216, 259, 530
SfcI CTRYAG 2 cut(s) 732, 759
SmiMI CAYNNNNRTG 2 cut(s) 162, 623
SmlI CTYRAG 2 cut(s) 178, 1130
SmoI CTYRAG 2 cut(s) 178, 1130
SphI GCATGC 1 cut(s) 622
Sse9I AATT 5 cut(s) 237, 288, 375, 566, 1012
SsiI CCGC 2 cut(s) 553, 1099
StyD4I CCNGG 2 cut(s) 679, 930
TaaI ACNGT 1 cut(s) 91
TaiI ACGT 3 cut(s) 253, 616, 777
TaqI TCGA 5 cut(s) 80, 606, 647, 902, 987
TasI AATT 5 cut(s) 237, 288, 375, 566, 1012
TatI WGTACW 3 cut(s) 333, 746, 1054
TauI GCSGC 1 cut(s) 1102
TfiI GAWTC 4 cut(s) 43, 385, 989, 999
Tru1I TTAA 5 cut(s) 108, 240, 404, 672, 975
Tru9I TTAA 5 cut(s) 108, 240, 404, 672, 975
TscAI CASTG 4 cut(s) 736, 768, 1153, 1209
TseFI GTSAC 4 cut(s) 202, 793, 841, 916
TseI GCWGC 4 cut(s) 339, 455, 458, 955
Tsp45I GTSAC 4 cut(s) 202, 793, 841, 916
TspDTI ATGAA 6 cut(s) 311, 438, 587, 790, 981, 1149
TspGWI ACGGA 2 cut(s) 90, 253
TspRI CASTG 4 cut(s) 736, 768, 1153, 1209
Tth111I GACNNNGTC 1 cut(s) 791
Van91I CCANNNNNTGG 1 cut(s) 683
VspI ATTAAT 1 cut(s) 404
XapI RAATTY 1 cut(s) 375
XceI RCATGY 2 cut(s) 622, 892
XcmI CCANNNNNNNNNTGG 1 cut(s) 552
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.