Rh1AG174700

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0000404
Physical Location & Seq
Reverse (-)
33478444 .. 33481118
2675 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG174700.1

Sequence Viewer

Length: 1311 bp
ATGGGGAGAGAGAGAGAGATGGAGGTTAGAGATGGCGACCATGAAGACGATACGACAAAAGCACTCTATGAGGCAGCAGTTGCAGGCTCAGTATCATCTTTTAAAACATTGATCCAGAAAGACCGTCTCATCCTCGACGAAGTTTCACTCACTCACTTCAGTGAAACTCCCCTCCACATCTCAGCTCTGCTCGGCAACATCGATTTCACCAAGGCTATTCTTACACATAACTGGAGACTTGCCACCGTGAGGGACTCCTTGAAACGCCTGCCGCTTCTCTTGGCAGCTGCCGAGGGCCAAAAGGAGATTGTTCAAGCTCTTCTGAAAGCCTACCCGGCCGCTTGCTTGTTTCCTGATCAGTATGGAAGAATCCCTCTTCACTATGCCGCTATGAGAGGACACGCTGAGGTCTTAGTTGAGTTGATGCACAAAATGCCCAAATCAGTCATGTTAAGGGTTCTTGATAGAGGAGACACGGTTTTCCACTTATGTGTTACTTATAACCAATTAGAATGCTTGAAGCTATTGGTGAAAGAGGTAGGGGACAATAGAGACCTCCTCAATGCTAGAGCTGGATGCGATGGTGGTATGACCATCCTCGAATTAGCTGTGATGCTAAGGCAAACTGAGACAATAAAATACTTGCGCTCGCTACCTGTTGTACGAGCAGAAGCAAAGAAAGATGGAAAAAACGGAAGGAAAAGTGAAAATAAGGTGAATGAGAAATGCTATAACAGTGGGACTTGGGTGGAAGAGAAACGCGGCATGCTGATGGTTGTGGCTACCATGATCTCAACAATGACATTCCAAGCTACAATTAACCCACCAGGTGGGTTCTGGCAAGAAATGAACACCAACTCGACTTTTGATGGAGCAATTATTTGCAATGTAACAAACCCTTGTGTTGCCGGAACTGCAGTCTCAAGCTACATACATACAGATTACTTCAACAACTTCCAGACTTACAATGCCATATGCTTCCTTTTTTCTTTGAGCATCACTCTTTTGCTTATAAGCGGATTTCCTCTCCGTAAACGAGTACTTGTCTGGCTCTTGTCAATAGTCATGTGTCTCACTCTCACATTCTTGGCGCTGACCTTCAGTGAAGGTGCAAAGATGGTAATACCCAAAAGTGTTGATTCTGAATATACTACCACTATCAGGATTGTGAGTTCTGTCACATTGTTTTGGGTTCCGCTACTTGGACTGGTTGCTTTATATAACGTTATTCGACTTCTCATTTGGCTCGTAAAGCTGTTGTGGCGCATAATCTTTCCCAAAAACAACAATGACAACCGTTCGCACGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

436

Amino Acids

49.05

Weight (kDa)

8.74

Isoelectric Point (pI)

33.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 22 - 109 1.5e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 92 - 179 1.6e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 126 - 216 3.6e-10 Ankyrin repeats (3 copies)
PGG PF13962 249 - 371 1.4e-17 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13950 AT4G13266
fragaria_vesca FvH4_7g06240 FvH4_7g06250 FvH4_7g06270
malus_domestica MD12G1213300.v1.1 MD12G1213900.v1.1 MD12G1214100.v1.1 MD12G1214700.v1.1 MD12G1214800.v1.1
prunus_persica Prupe.2G107700_v2.0.a1 Prupe.3G142200_v2.0.a1 Prupe.3G154100_v2.0.a1 Prupe.3G154300_v2.0.a1 Prupe.3G154900_v2.0.a1 Prupe.3G155100_v2.0.a1
pyrus_communis pycom12g20010 pycom12g20080 pycom12g20090 pycom12g20100
rosa_chinensis RchiOBHm_Chr1g0341001 RchiOBHm_Chr1g0341021 RchiOBHm_Chr1g0341891 RchiOBHm_Chr1g0342341 RchiOBHm_Chr3g0490431
rosa_laevigata RLG00000022889 RLG00000029016 RLG00000029039 RLG00000029059 RLG00000029061 RLG00000029065 RLG00000029115 RLG00000029155 RLG00000033311 RLG00000033312
rosa_multiflora Rmu_sc0000404.1_g000006 Rmu_sc0001836.1_g000015 Rmu_sc0001836.1_g000050 Rmu_sc0002200.1_g000003 Rmu_sc0003862.1_g000020 Rmu_sc0005193.1_g000001 Rmu_sc0005193.1_g000002 Rmu_sc0008453.1_g000008 Rmu_sc0008453.1_g000009 Rmu_sc0008453.1_g000010 Rmu_sc0008862.1_g000001 Rmu_sc0011195.1_g000003 Rmu_sc0014077.1_g000001
rosa_roxburghii Rroxscaffold_4G00311670 Rroxscaffold_4G00311730 Rroxscaffold_4G00311750 Rroxscaffold_4G00312290 Rroxscaffold_6G00393430
rosa_rugosa Rorug01G0153800.1 Rorug01G0153900.1 Rorug01G0160100.1 Rorug03G0243800
rosa_samantha Rh1AG166400 Rh1AG168200 Rh1AG174100 Rh1AG174700 Rh1AG179000 Rh1BG132600 Rh1BG136500 Rh1BG142200 Rh1BG142800 Rh1BG143200 Rh1BG146200 Rh1BG147000 Rh1BG147100 Rh1CG154800 Rh1CG161900 Rh1CG162700 Rh1CG166000 Rh1DG167500 Rh1DG169900 Rh1DG174000 Rh1DG174400 Rh1DG174700 Rh1DG174800 Rh1DG178200 Rh1DG179000 Rh1DG179100 Rh3AG292800 Rh3BG329600 Rh3CG326900 Rh3DG326200 Rh5BG218300 Rh7CG326800 Rh7DG307100
rosa_wichuraiana Rw0G006190 Rw0G007830 Rw0G009970 Rw0G009980 Rw1G013880 Rw1G014000 Rw1G014500 Rw1G014560 Rw1G014920 Rw3G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 501, 1013
AccB7I CCANNNNNTGG 1 cut(s) 830
AccII CGCG 1 cut(s) 762
AciI CCGC 6 cut(s) 272, 339, 387, 762, 1017, 1196
AclI AACGTT 1 cut(s) 1224
AclWI GGATC 1 cut(s) 106
AcoI YGGCCR 1 cut(s) 336
AcuI CTGAAG 2 cut(s) 142, 1084
AdeI CACNNNGTG 1 cut(s) 830
AfaI GTAC 2 cut(s) 663, 1041
AfiI CCNNNNNNNGG 4 cut(s) 249, 830, 1161, 1202
AgsI TTSAA 4 cut(s) 262, 314, 520, 949
AjiI CACGTC 1 cut(s) 1306
AjnI CCWGG 1 cut(s) 826
AleI CACNNNNGTG 2 cut(s) 159, 489
AloI GAACNNNNNNTCC 2 cut(s) 1156, 1188
AluBI AGCT 9 cut(s) 185, 287, 317, 523, 572, 608, 812, 927, 1255
AluI AGCT 9 cut(s) 185, 287, 317, 523, 572, 608, 812, 927, 1255
Alw26I GTCTC 7 cut(s) 131, 229, 465, 546, 623, 925, 1076
AlwI GGATC 1 cut(s) 106
AoxI GGCC 2 cut(s) 295, 336
ApeKI GCWGC 3 cut(s) 74, 284, 287
ArsI GACNNNNNNTTYG 2 cut(s) 1224, 1256
AspLEI GCGC 3 cut(s) 648, 1093, 1266
AspS9I GGNCC 1 cut(s) 295
AsuC2I CCSGG 1 cut(s) 335
AsuHPI GGTGA 3 cut(s) 199, 541, 727
BbsI GAAGAC 1 cut(s) 51
BbvCI CCTCAGC 1 cut(s) 405
BbvI GCAGC 3 cut(s) 86, 274, 296
BccI CCATC 8 cut(s) 13, 26, 575, 602, 677, 766, 863, 1111
BciT130I CCWGG 1 cut(s) 828
BclI TGATCA 1 cut(s) 355
BcnI CCSGG 1 cut(s) 335
BcoDI GTCTC 7 cut(s) 131, 229, 465, 546, 623, 925, 1076
BfaI CTAG 1 cut(s) 567
BfmI CTRYAG 1 cut(s) 915
BfoI RGCGCY 1 cut(s) 1094
BglI GCCNNNNNGGC 1 cut(s) 335
BisI GCNGC 7 cut(s) 75, 272, 285, 288, 339, 387, 763
BlsI GCNGC 7 cut(s) 76, 273, 286, 289, 340, 388, 764
BmcAI AGTACT 1 cut(s) 1041
Bme1390I CCNGG 2 cut(s) 335, 828
BmgBI CACGTC 1 cut(s) 1306
BmgT120I GGNCC 1 cut(s) 295
BmiI GGNNCC 1 cut(s) 1194
BmrFI CCNGG 2 cut(s) 335, 828
BmsI GCATC 4 cut(s) 414, 566, 603, 1005
BpiI GAAGAC 1 cut(s) 51
BplI GAGNNNNNCTC 4 cut(s) 543, 575, 985, 1017
BpmI CTGGAG 1 cut(s) 253
Bpu10I CCTNAGC 2 cut(s) 405, 617
BpuEI CTTGAG 1 cut(s) 907
BpuMI CCSGG 1 cut(s) 335
Bsa29I ATCGAT 1 cut(s) 201
BsaI GGTCTC 1 cut(s) 546
BsaJI CCNNGG 2 cut(s) 210, 291
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bsc4I CCNNNNNNNGG 4 cut(s) 249, 830, 1161, 1202
Bse1I ACTGG 2 cut(s) 236, 1212
Bse3DI GCAATG 1 cut(s) 892
BseBI CCWGG 1 cut(s) 828
BseCI ATCGAT 1 cut(s) 201
BseDI CCNNGG 2 cut(s) 210, 291
BseGI GGATG 3 cut(s) 129, 581, 594
BseLI CCNNNNNNNGG 4 cut(s) 249, 830, 1161, 1202
BseMI GCAATG 1 cut(s) 892
BseMII CTCAG 4 cut(s) 102, 195, 396, 618
BseNI ACTGG 2 cut(s) 236, 1212
BseRI GAGGAG 2 cut(s) 483, 548
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 3 cut(s) 86, 274, 296
Bsh1236I CGCG 1 cut(s) 762
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 2 cut(s) 297, 338
BshVI ATCGAT 1 cut(s) 201
BsiEI CGRYCG 1 cut(s) 339
BsiSI CCGG 2 cut(s) 335, 909
BslFI GGGAC 3 cut(s) 266, 557, 754
BslI CCNNNNNNNGG 4 cut(s) 249, 830, 1161, 1202
BsmAI GTCTC 7 cut(s) 131, 229, 465, 546, 623, 925, 1076
BsmBI CGTCTC 1 cut(s) 131
BsmFI GGGAC 3 cut(s) 266, 557, 754
BsmI GAATGC 1 cut(s) 518
BsnI GGCC 2 cut(s) 297, 338
Bso31I GGTCTC 1 cut(s) 546
Bsp143I GATC 3 cut(s) 111, 355, 789
BspACI CCGC 6 cut(s) 272, 339, 387, 762, 1017, 1196
BspANI GGCC 2 cut(s) 297, 338
BspCNI CTCAG 4 cut(s) 101, 194, 397, 619
BspDI ATCGAT 1 cut(s) 201
BspFNI CGCG 1 cut(s) 762
BspLI GGNNCC 1 cut(s) 1194
BspMAI CTGCAG 1 cut(s) 919
BspPI GGATC 1 cut(s) 106
BspQI GCTCTTC 1 cut(s) 324
BspTNI GGTCTC 1 cut(s) 546
BsrDI GCAATG 1 cut(s) 892
BsrI ACTGG 2 cut(s) 236, 1212
BssECI CCNNGG 2 cut(s) 210, 291
BssMI GATC 3 cut(s) 111, 355, 789
BssT1I CCWWGG 1 cut(s) 210
Bst2UI CCWGG 1 cut(s) 828
Bst4CI ACNGT 5 cut(s) 125, 247, 478, 737, 1298
Bst6I CTCTTC 3 cut(s) 324, 381, 747
BstAPI GCANNNNNTGC 2 cut(s) 80, 433
BstC8I GCNNGC 5 cut(s) 85, 269, 343, 650, 767
BstDEI CTNAG 6 cut(s) 88, 181, 405, 412, 617, 627
BstF5I GGATG 3 cut(s) 129, 581, 594
BstFNI CGCG 1 cut(s) 762
BstH2I RGCGCY 1 cut(s) 1094
BstHHI GCGC 3 cut(s) 648, 1093, 1266
BstKTI GATC 3 cut(s) 114, 358, 792
BstMAI GTCTC 7 cut(s) 131, 229, 465, 546, 623, 925, 1076
BstMBI GATC 3 cut(s) 111, 355, 789
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 6 cut(s) 80, 335, 433, 914, 1252, 1261
BstNI CCWGG 1 cut(s) 828
BstNSI RCATGY 1 cut(s) 769
BstSCI CCNGG 2 cut(s) 333, 826
BstSFI CTRYAG 1 cut(s) 915
BstUI CGCG 1 cut(s) 762
BstV1I GCAGC 3 cut(s) 86, 274, 296
BstV2I GAAGAC 1 cut(s) 51
BstZI CGGCCG 1 cut(s) 336
Bsu15I ATCGAT 1 cut(s) 201
BsuRI GGCC 2 cut(s) 297, 338
BsuTUI ATCGAT 1 cut(s) 201
BtgZI GCGATG 1 cut(s) 594
BtrI CACGTC 1 cut(s) 1306
BtsCI GGATG 3 cut(s) 129, 581, 594
BtsIMutI CAGTG 3 cut(s) 166, 742, 1108
Cac8I GCNNGC 5 cut(s) 85, 269, 343, 650, 767
CfoI GCGC 3 cut(s) 648, 1093, 1266
Cfr13I GGNCC 1 cut(s) 295
ClaI ATCGAT 1 cut(s) 201
CsiI ACCWGGT 1 cut(s) 826
Csp6I GTAC 2 cut(s) 662, 1040
CviAII CATG 5 cut(s) 41, 448, 766, 787, 1066
CviQI GTAC 2 cut(s) 662, 1040
DdeI CTNAG 6 cut(s) 88, 181, 405, 412, 617, 627
DpnI GATC 3 cut(s) 113, 357, 791
DpnII GATC 3 cut(s) 111, 355, 789
DraI TTTAAA 1 cut(s) 103
DraIII CACNNNGTG 1 cut(s) 830
EaeI YGGCCR 1 cut(s) 336
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 3 cut(s) 324, 381, 747
EarI CTCTTC 3 cut(s) 324, 381, 747
EclXI CGGCCG 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 210
Eco31I GGTCTC 1 cut(s) 546
Eco52I CGGCCG 1 cut(s) 336
Eco57I CTGAAG 2 cut(s) 142, 1084
EcoRII CCWGG 1 cut(s) 826
EcoT14I CCWWGG 1 cut(s) 210
ErhI CCWWGG 1 cut(s) 210
Esp3I CGTCTC 1 cut(s) 131
FaeI CATG 5 cut(s) 44, 451, 769, 790, 1069
FaqI GGGAC 3 cut(s) 266, 557, 754
FatI CATG 5 cut(s) 40, 447, 765, 786, 1065
FauNDI CATATG 1 cut(s) 974
FbaI TGATCA 1 cut(s) 355
Fnu4HI GCNGC 7 cut(s) 75, 272, 285, 288, 339, 387, 763
FokI GGATG 3 cut(s) 116, 581, 588
Fsp4HI GCNGC 7 cut(s) 75, 272, 285, 288, 339, 387, 763
FspBI CTAG 1 cut(s) 567
GlaI GCGC 3 cut(s) 647, 1092, 1265
GluI GCNGC 7 cut(s) 75, 272, 285, 288, 339, 387, 763
GsuI CTGGAG 1 cut(s) 253
HaeII RGCGCY 1 cut(s) 1094
HaeIII GGCC 2 cut(s) 297, 338
HapII CCGG 2 cut(s) 335, 909
HhaI GCGC 3 cut(s) 648, 1093, 1266
Hin1II CATG 5 cut(s) 44, 451, 769, 790, 1069
Hin6I GCGC 3 cut(s) 646, 1091, 1264
HinP1I GCGC 3 cut(s) 646, 1091, 1264
HinfI GANTC 3 cut(s) 254, 369, 1139
HpaII CCGG 2 cut(s) 335, 909
HphI GGTGA 3 cut(s) 199, 541, 727
Hpy166II GTNNAC 1 cut(s) 1034
Hpy188I TCNGA 2 cut(s) 324, 1144
Hpy188III TCNNGA 5 cut(s) 115, 353, 461, 958, 1162
Hpy8I GTNNAC 1 cut(s) 1034
Hpy99I CGWCG 1 cut(s) 140
HpyAV CCTTC 3 cut(s) 690, 1100, 1108
HpyCH4III ACNGT 5 cut(s) 125, 247, 478, 737, 1298
HpyCH4IV ACGT 2 cut(s) 1224, 1305
HpyCH4V TGCA 5 cut(s) 83, 427, 885, 917, 1112
HpyF10VI GCNNNNNNNGC 6 cut(s) 80, 335, 433, 914, 1252, 1261
HpyF3I CTNAG 6 cut(s) 88, 181, 405, 412, 617, 627
HpySE526I ACGT 2 cut(s) 1224, 1305
Hsp92II CATG 5 cut(s) 44, 451, 769, 790, 1069
HspAI GCGC 3 cut(s) 646, 1091, 1264
Ksp22I TGATCA 1 cut(s) 355
Kzo9I GATC 3 cut(s) 111, 355, 789
LguI GCTCTTC 1 cut(s) 324
LmnI GCTCC 1 cut(s) 872
Lsp1109I GCAGC 3 cut(s) 86, 274, 296
LweI GCATC 4 cut(s) 414, 566, 603, 1005
MabI ACCWGGT 1 cut(s) 826
MaeI CTAG 1 cut(s) 567
MaeII ACGT 2 cut(s) 1224, 1305
MaeIII GTNAC 3 cut(s) 493, 889, 1177
MalI GATC 3 cut(s) 113, 357, 791
MboI GATC 3 cut(s) 111, 355, 789
MboII GAAGA 5 cut(s) 56, 311, 368, 378, 764
MluCI AATT 4 cut(s) 506, 602, 816, 876
MlyI GAGTC 1 cut(s) 248
MseI TTAA 3 cut(s) 102, 452, 819
MslI CAYNNNNRTG 3 cut(s) 159, 489, 770
MspA1I CMGCKG 1 cut(s) 287
MspI CCGG 2 cut(s) 335, 909
MspR9I CCNGG 2 cut(s) 335, 828
Mva1269I GAATGC 1 cut(s) 518
MvaI CCWGG 1 cut(s) 828
MvnI CGCG 1 cut(s) 762
MwoI GCNNNNNNNGC 6 cut(s) 80, 335, 433, 914, 1252, 1261
NciI CCSGG 1 cut(s) 335
NdeI CATATG 1 cut(s) 974
NdeII GATC 3 cut(s) 111, 355, 789
NlaIII CATG 5 cut(s) 44, 451, 769, 790, 1069
NlaIV GGNNCC 1 cut(s) 1194
NmeAIII GCCGAG 2 cut(s) 171, 316
NmuCI GTSAC 1 cut(s) 1177
NspI RCATGY 1 cut(s) 769
OliI CACNNNNGTG 2 cut(s) 159, 489
PaeI GCATGC 1 cut(s) 769
PciSI GCTCTTC 1 cut(s) 324
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 518
PfeI GAWTC 2 cut(s) 369, 1139
PflMI CCANNNNNTGG 1 cut(s) 830
PkrI GCNGC 7 cut(s) 76, 273, 286, 289, 340, 388, 764
PleI GAGTC 1 cut(s) 248
PpsI GAGTC 1 cut(s) 248
PsiI TTATAA 2 cut(s) 501, 1013
Psp1406I AACGTT 1 cut(s) 1224
Psp6I CCWGG 1 cut(s) 826
PspGI CCWGG 1 cut(s) 826
PspN4I GGNNCC 1 cut(s) 1194
PspPI GGNCC 1 cut(s) 295
PstI CTGCAG 1 cut(s) 919
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 2 cut(s) 663, 1041
RsaNI GTAC 2 cut(s) 662, 1040
RseI CAYNNNNRTG 3 cut(s) 159, 489, 770
SapI GCTCTTC 1 cut(s) 324
SaqAI TTAA 3 cut(s) 102, 452, 819
SatI GCNGC 7 cut(s) 75, 272, 285, 288, 339, 387, 763
Sau3AI GATC 3 cut(s) 111, 355, 789
Sau96I GGNCC 1 cut(s) 295
ScaI AGTACT 1 cut(s) 1041
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 2 cut(s) 335, 828
SexAI ACCWGGT 1 cut(s) 826
SfaNI GCATC 4 cut(s) 414, 566, 603, 1005
SfcI CTRYAG 1 cut(s) 915
SmiMI CAYNNNNRTG 3 cut(s) 159, 489, 770
SmlI CTYRAG 1 cut(s) 922
SmoI CTYRAG 1 cut(s) 922
SphI GCATGC 1 cut(s) 769
Sse9I AATT 4 cut(s) 506, 602, 816, 876
SsiI CCGC 6 cut(s) 272, 339, 387, 762, 1017, 1196
SspMI CTAG 1 cut(s) 567
StyD4I CCNGG 2 cut(s) 333, 826
StyI CCWWGG 1 cut(s) 210
TaaI ACNGT 5 cut(s) 125, 247, 478, 737, 1298
TaiI ACGT 2 cut(s) 1227, 1308
TaqI TCGA 5 cut(s) 135, 201, 600, 860, 1231
TasI AATT 4 cut(s) 506, 602, 816, 876
TatI WGTACW 1 cut(s) 1039
TauI GCSGC 4 cut(s) 274, 341, 389, 765
TfiI GAWTC 2 cut(s) 369, 1139
Tru1I TTAA 3 cut(s) 102, 452, 819
Tru9I TTAA 3 cut(s) 102, 452, 819
TscAI CASTG 3 cut(s) 166, 742, 1108
TseFI GTSAC 1 cut(s) 1177
TseI GCWGC 3 cut(s) 74, 284, 287
Tsp45I GTSAC 1 cut(s) 1177
TspDTI ATGAA 2 cut(s) 57, 863
TspGWI ACGGA 2 cut(s) 708, 1019
TspRI CASTG 3 cut(s) 166, 742, 1108
Van91I CCANNNNNTGG 1 cut(s) 830
XceI RCATGY 1 cut(s) 769
XcmI CCANNNNNNNNNTGG 1 cut(s) 834
XspI CTAG 1 cut(s) 567
ZrmI AGTACT 1 cut(s) 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.