MD12G1214800.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000404
Physical Location & Seq
Forward (+)
29335151 .. 29335810
660 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1214800.v1.1.491

Sequence Viewer

Length: 660 bp
ATGGAGATCACAAGGCATGATGAAGATGAAGTATACGAGGCATCACGGACTGGGGATGTGGAATACTTAAACAGATTGATTGAAAAAGACCCAGAAATTCCAAGGAGAATATCCCTGCAGACCGGAAAAACCGGAACCCCCTTGCATGTTTCGGCTTTGCTCGGCCACGCTGAGTTTACCAAATCCCTTTGCACTAAAAATCCCAAACTTGCAGAGCGAGTGGACGCCGATGGACGCACGCCCCTGCATTTGGCTTCTGCTGAGGGCCAGAAGGAGACTGTCGAAGCTTTGTTATCAGTGTATGCTAATGCGTGCTTGCGTTGCGATGAAAAGGGAAGAATCCCTCTTCACTATGCAGCCATGAATGGAGAAGTTGAGGTGCTTCAGAAGTTGATTGATAAAAATCCTGAGTCCATTTATGTCAAAGTTGAAAACAGATCAAATGAAACAGTTTTGCACTTGTGTATTATACACAACCAGTTAAAGTGCTTGAAATTGTTGGTTGAAAGACTGTTGGTTGAAAGAGACAACAGAAATGATGAGTTCCTCAACTCAAAAGCTGGCTGTGATGGTGGTGTGACCATCCTGCGCTTAGCTTTGATGCTAAGGCAAATTAAGGTACGTATATATATTTCCATTTTCTATATTGGATATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

220

Amino Acids

24.8

Weight (kDa)

6.4

Isoelectric Point (pI)

35.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 14 - 103 6.1e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 108 - 170 1.3e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 116 - 168 8.9e-08 Ankyrin repeats (many copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13950 AT4G13266
fragaria_vesca FvH4_7g06240 FvH4_7g06250 FvH4_7g06270
malus_domestica MD12G1213300.v1.1 MD12G1213900.v1.1 MD12G1214100.v1.1 MD12G1214700.v1.1 MD12G1214800.v1.1
prunus_persica Prupe.2G107700_v2.0.a1 Prupe.3G142200_v2.0.a1 Prupe.3G154100_v2.0.a1 Prupe.3G154300_v2.0.a1 Prupe.3G154900_v2.0.a1 Prupe.3G155100_v2.0.a1
pyrus_communis pycom12g20010 pycom12g20080 pycom12g20090 pycom12g20100
rosa_chinensis RchiOBHm_Chr1g0341001 RchiOBHm_Chr1g0341021 RchiOBHm_Chr1g0341891 RchiOBHm_Chr1g0342341 RchiOBHm_Chr3g0490431
rosa_laevigata RLG00000022889 RLG00000029016 RLG00000029039 RLG00000029059 RLG00000029061 RLG00000029065 RLG00000029115 RLG00000029155 RLG00000033311 RLG00000033312
rosa_multiflora Rmu_sc0000404.1_g000006 Rmu_sc0001836.1_g000015 Rmu_sc0001836.1_g000050 Rmu_sc0002200.1_g000003 Rmu_sc0003862.1_g000020 Rmu_sc0005193.1_g000001 Rmu_sc0005193.1_g000002 Rmu_sc0008453.1_g000008 Rmu_sc0008453.1_g000009 Rmu_sc0008453.1_g000010 Rmu_sc0008862.1_g000001 Rmu_sc0011195.1_g000003 Rmu_sc0014077.1_g000001
rosa_roxburghii Rroxscaffold_4G00311670 Rroxscaffold_4G00311730 Rroxscaffold_4G00311750 Rroxscaffold_4G00312290 Rroxscaffold_6G00393430
rosa_rugosa Rorug01G0153800.1 Rorug01G0153900.1 Rorug01G0160100.1 Rorug03G0243800
rosa_samantha Rh1AG166400 Rh1AG168200 Rh1AG174100 Rh1AG174700 Rh1AG179000 Rh1BG132600 Rh1BG136500 Rh1BG142200 Rh1BG142800 Rh1BG143200 Rh1BG146200 Rh1BG147000 Rh1BG147100 Rh1CG154800 Rh1CG161900 Rh1CG162700 Rh1CG166000 Rh1DG167500 Rh1DG169900 Rh1DG174000 Rh1DG174400 Rh1DG174700 Rh1DG174800 Rh1DG178200 Rh1DG179000 Rh1DG179100 Rh3AG292800 Rh3BG329600 Rh3CG326900 Rh3DG326200 Rh5BG218300 Rh7CG326800 Rh7DG307100
rosa_wichuraiana Rw0G006190 Rw0G007830 Rw0G009970 Rw0G009980 Rw1G013880 Rw1G014000 Rw1G014500 Rw1G014560 Rw1G014920 Rw3G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 33
AcoI YGGCCR 1 cut(s) 163
AcsI RAATTY 1 cut(s) 96
AcuI CTGAAG 1 cut(s) 368
AcyI GRCGYC 1 cut(s) 225
AfaI GTAC 1 cut(s) 621
AfiI CCNNNNNNNGG 1 cut(s) 250
AgsI TTSAA 5 cut(s) 83, 431, 493, 506, 521
AluBI AGCT 3 cut(s) 287, 560, 596
AluI AGCT 3 cut(s) 287, 560, 596
Alw26I GTCTC 2 cut(s) 269, 519
AoxI GGCC 2 cut(s) 163, 265
ApeKI GCWGC 1 cut(s) 356
ApoI RAATTY 1 cut(s) 96
AspLEI GCGC 1 cut(s) 591
AspS9I GGNCC 1 cut(s) 265
BbvCI CCTCAGC 1 cut(s) 261
BbvI GCAGC 1 cut(s) 368
BccI CCATC 3 cut(s) 224, 563, 590
BcoDI GTCTC 2 cut(s) 269, 519
BfmI CTRYAG 1 cut(s) 116
BisI GCNGC 1 cut(s) 357
BlpI GCTNAGC 1 cut(s) 592
BlsI GCNGC 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 1 cut(s) 136
BmrI ACTGGG 1 cut(s) 60
BmsI GCATC 2 cut(s) 50, 591
BmuI ACTGGG 1 cut(s) 60
Bpu10I CCTNAGC 2 cut(s) 261, 605
Bpu1102I GCTNAGC 1 cut(s) 592
BsaAI YACGTR 1 cut(s) 623
BsaBI GATNNNNATC 1 cut(s) 402
BsaHI GRCGYC 1 cut(s) 225
BsaJI CCNNGG 1 cut(s) 101
BsaWI WCCGGW 2 cut(s) 122, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 250
Bse1I ACTGG 2 cut(s) 55, 478
Bse8I GATNNNNATC 1 cut(s) 402
BseDI CCNNGG 1 cut(s) 101
BseGI GGATG 2 cut(s) 61, 582
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 1 cut(s) 250
BseMII CTCAG 3 cut(s) 162, 252, 399
BseNI ACTGG 2 cut(s) 55, 478
BseXI GCAGC 1 cut(s) 368
BshFI GGCC 2 cut(s) 165, 267
BsiSI CCGG 2 cut(s) 123, 132
BslI CCNNNNNNNGG 1 cut(s) 250
BsmAI GTCTC 2 cut(s) 269, 519
BsnI GGCC 2 cut(s) 165, 267
Bsp143I GATC 2 cut(s) 6, 437
Bsp1720I GCTNAGC 1 cut(s) 592
BspANI GGCC 2 cut(s) 165, 267
BspCNI CTCAG 3 cut(s) 163, 253, 400
BspLI GGNNCC 1 cut(s) 136
BspMAI CTGCAG 1 cut(s) 120
BsrI ACTGG 2 cut(s) 55, 478
BssECI CCNNGG 1 cut(s) 101
BssMI GATC 2 cut(s) 6, 437
BssNAI GTATAC 1 cut(s) 34
BssNI GRCGYC 1 cut(s) 225
BssT1I CCWWGG 1 cut(s) 101
Bst1107I GTATAC 1 cut(s) 34
Bst4CI ACNGT 3 cut(s) 280, 451, 513
Bst6I CTCTTC 1 cut(s) 351
BstACI GRCGYC 1 cut(s) 225
BstBAI YACGTR 1 cut(s) 623
BstC8I GCNNGC 4 cut(s) 239, 313, 317, 562
BstDEI CTNAG 5 cut(s) 171, 261, 408, 592, 605
BstF5I GGATG 2 cut(s) 61, 582
BstHHI GCGC 1 cut(s) 591
BstKTI GATC 2 cut(s) 9, 440
BstMAI GTCTC 2 cut(s) 269, 519
BstMBI GATC 2 cut(s) 6, 437
BstMWI GCNNNNNNNGC 1 cut(s) 321
BstNSI RCATGY 1 cut(s) 149
BstSFI CTRYAG 1 cut(s) 116
BstSNI TACGTA 1 cut(s) 623
BstV1I GCAGC 1 cut(s) 368
BstZ17I GTATAC 1 cut(s) 34
BsuRI GGCC 2 cut(s) 165, 267
BtgZI GCGATG 1 cut(s) 339
BtsCI GGATG 2 cut(s) 61, 582
BtsIMutI CAGTG 1 cut(s) 303
Cac8I GCNNGC 4 cut(s) 239, 313, 317, 562
CfoI GCGC 1 cut(s) 591
Cfr13I GGNCC 1 cut(s) 265
CseI GACGC 2 cut(s) 233, 243
Csp6I GTAC 1 cut(s) 620
CviAII CATG 3 cut(s) 17, 146, 361
CviJI RGCY 9 cut(s) 155, 165, 254, 267, 287, 359, 560, 564, 596
CviKI_1 RGCY 9 cut(s) 155, 165, 254, 267, 287, 359, 560, 564, 596
CviQI GTAC 1 cut(s) 620
DdeI CTNAG 5 cut(s) 171, 261, 408, 592, 605
DpnI GATC 2 cut(s) 8, 439
DpnII GATC 2 cut(s) 6, 437
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 351
EarI CTCTTC 1 cut(s) 351
Eco105I TACGTA 1 cut(s) 623
Eco130I CCWWGG 1 cut(s) 101
Eco57I CTGAAG 1 cut(s) 368
EcoT14I CCWWGG 1 cut(s) 101
ErhI CCWWGG 1 cut(s) 101
FaeI CATG 3 cut(s) 20, 149, 364
FatI CATG 3 cut(s) 16, 145, 360
FblI GTMKAC 1 cut(s) 33
Fnu4HI GCNGC 1 cut(s) 357
FokI GGATG 2 cut(s) 68, 569
Fsp4HI GCNGC 1 cut(s) 357
GlaI GCGC 1 cut(s) 590
GluI GCNGC 1 cut(s) 357
HaeIII GGCC 2 cut(s) 165, 267
HapII CCGG 2 cut(s) 123, 132
HgaI GACGC 2 cut(s) 233, 243
HhaI GCGC 1 cut(s) 591
Hin1I GRCGYC 1 cut(s) 225
Hin1II CATG 3 cut(s) 20, 149, 364
Hin6I GCGC 1 cut(s) 589
HinP1I GCGC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 285
HinfI GANTC 2 cut(s) 339, 410
HpaII CCGG 2 cut(s) 123, 132
Hpy166II GTNNAC 3 cut(s) 34, 177, 223
Hpy188I TCNGA 1 cut(s) 387
Hpy188III TCNNGA 1 cut(s) 407
Hpy8I GTNNAC 3 cut(s) 34, 177, 223
HpyAV CCTTC 1 cut(s) 265
HpyCH4III ACNGT 3 cut(s) 280, 451, 513
HpyCH4IV ACGT 1 cut(s) 622
HpyCH4V TGCA 7 cut(s) 118, 145, 192, 212, 247, 356, 457
HpyF10VI GCNNNNNNNGC 1 cut(s) 321
HpyF3I CTNAG 5 cut(s) 171, 261, 408, 592, 605
HpySE526I ACGT 1 cut(s) 622
Hsp92I GRCGYC 1 cut(s) 225
Hsp92II CATG 3 cut(s) 20, 149, 364
HspAI GCGC 1 cut(s) 589
Kzo9I GATC 2 cut(s) 6, 437
Lsp1109I GCAGC 1 cut(s) 368
LweI GCATC 2 cut(s) 50, 591
MaeII ACGT 1 cut(s) 622
MaeIII GTNAC 1 cut(s) 577
MalI GATC 2 cut(s) 8, 439
MboI GATC 2 cut(s) 6, 437
MboII GAAGA 3 cut(s) 35, 338, 348
MluCI AATT 3 cut(s) 96, 494, 612
MlyI GAGTC 1 cut(s) 419
MnlI CCTC 5 cut(s) 31, 256, 354, 370, 557
MseI TTAA 4 cut(s) 68, 482, 615, 658
MspI CCGG 2 cut(s) 123, 132
MwoI GCNNNNNNNGC 1 cut(s) 321
NdeII GATC 2 cut(s) 6, 437
NlaIII CATG 3 cut(s) 20, 149, 364
NlaIV GGNNCC 1 cut(s) 136
NmeAIII GCCGAG 1 cut(s) 141
NmuCI GTSAC 1 cut(s) 577
NspI RCATGY 1 cut(s) 149
PfeI GAWTC 1 cut(s) 339
PkrI GCNGC 1 cut(s) 358
PleI GAGTC 1 cut(s) 418
PpsI GAGTC 1 cut(s) 418
Ppu21I YACGTR 1 cut(s) 623
PspN4I GGNNCC 1 cut(s) 136
PspPI GGNCC 1 cut(s) 265
PstI CTGCAG 1 cut(s) 120
RsaI GTAC 1 cut(s) 621
RsaNI GTAC 1 cut(s) 620
SaqAI TTAA 4 cut(s) 68, 482, 615, 658
SatI GCNGC 1 cut(s) 357
Sau3AI GATC 2 cut(s) 6, 437
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 1 cut(s) 419
SetI ASST 6 cut(s) 289, 381, 562, 598, 621, 625
SfaNI GCATC 2 cut(s) 50, 591
SfcI CTRYAG 1 cut(s) 116
SnaBI TACGTA 1 cut(s) 623
Sse9I AATT 3 cut(s) 96, 494, 612
StyI CCWWGG 1 cut(s) 101
TaaI ACNGT 3 cut(s) 280, 451, 513
TaiI ACGT 1 cut(s) 625
TaqI TCGA 1 cut(s) 282
TasI AATT 3 cut(s) 96, 494, 612
TfiI GAWTC 1 cut(s) 339
Tru1I TTAA 4 cut(s) 68, 482, 615, 658
Tru9I TTAA 4 cut(s) 68, 482, 615, 658
TscAI CASTG 1 cut(s) 303
TseFI GTSAC 1 cut(s) 577
TseI GCWGC 1 cut(s) 356
Tsp45I GTSAC 1 cut(s) 577
TspDTI ATGAA 5 cut(s) 36, 42, 342, 377, 459
TspGWI ACGGA 1 cut(s) 61
TspRI CASTG 1 cut(s) 303
XapI RAATTY 1 cut(s) 96
XceI RCATGY 1 cut(s) 149
XmiI GTMKAC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.