pycom12g20090

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000404
Physical Location & Seq
Forward (+)
21727309 .. 21728133
825 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g20090.1

Sequence Viewer

Length: 825 bp
ATGTCTCTGACCATGTCAGATATCTTAGAGTACAGCAGCGTTGCAAGAGAGGACTTTAGCAGAAGCTTAGAAATTCAACAGATTTTGATGGACGCAGGATTAATCAGAAGACAAAATAATGAAAATGATAATCCTAACTCAGCTGTCGCCGCTGCTGCTGATGTTGTATCACCAAGTCCAAGAAGGGTTGAGAAGAAATGGTGGTTCTTGACAGAATTGAAGAACTGGTATGGATATCCGCGTGATTGGGTGAAGGACTTACGTGGCATGCTGATGGTTGTGGCTACGATGATCTCGACCATGACTTTTCAAGCCGTAGTCAACCCACCTGGAGGTGTTTGGGACCATAATGATACAAACACTACCCTTGGTAATATAACAATTTGCAGTGAATCGAGAGTATGTAAAGCTGGAACTGCAGTGTTAAGCTACTACGGTAATGCTTCCCACGTTGACTTCGCCGATTTCATAGCATACAATACCATCTCGTTCTTTGCTTCTGTGAGTGTCACCATTTTGCTCGTTAGTGGACTTCCTCTCCACAATCGGCTCTCAACGTGGCTCTTACCGATGTTCATGTGCTACGCTCTCTTATTCTTGGCATGCACCTACCTAGCGGCGCTGTCCATAATCGACCCTAAATACTCTTGGGTTTCTTGGGTCGCGCCATTCAGTTTTCTTTCGGCTGCGTTGGTGGTCAGCGGTCTACTTCAGGGTATCCGGTCCGTGTCCTGGTTAACATCAGTTCGCAATAATATGATCGGCATTTTTTCACGTATCGATCCAACGCGTTTTGAAGAGAATGATGCGCCTGCAGTTGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

275

Amino Acids

30.56

Weight (kDa)

5.62

Isoelectric Point (pI)

42.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 82 - 210 6.9e-18 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13950 AT4G13266
fragaria_vesca FvH4_7g06240 FvH4_7g06250 FvH4_7g06270
malus_domestica MD12G1213300.v1.1 MD12G1213900.v1.1 MD12G1214100.v1.1 MD12G1214700.v1.1 MD12G1214800.v1.1
prunus_persica Prupe.2G107700_v2.0.a1 Prupe.3G142200_v2.0.a1 Prupe.3G154100_v2.0.a1 Prupe.3G154300_v2.0.a1 Prupe.3G154900_v2.0.a1 Prupe.3G155100_v2.0.a1
pyrus_communis pycom12g20010 pycom12g20080 pycom12g20090 pycom12g20100
rosa_chinensis RchiOBHm_Chr1g0341001 RchiOBHm_Chr1g0341021 RchiOBHm_Chr1g0341891 RchiOBHm_Chr1g0342341 RchiOBHm_Chr3g0490431
rosa_laevigata RLG00000022889 RLG00000029016 RLG00000029039 RLG00000029059 RLG00000029061 RLG00000029065 RLG00000029115 RLG00000029155 RLG00000033311 RLG00000033312
rosa_multiflora Rmu_sc0000404.1_g000006 Rmu_sc0001836.1_g000015 Rmu_sc0001836.1_g000050 Rmu_sc0002200.1_g000003 Rmu_sc0003862.1_g000020 Rmu_sc0005193.1_g000001 Rmu_sc0005193.1_g000002 Rmu_sc0008453.1_g000008 Rmu_sc0008453.1_g000009 Rmu_sc0008453.1_g000010 Rmu_sc0008862.1_g000001 Rmu_sc0011195.1_g000003 Rmu_sc0014077.1_g000001
rosa_roxburghii Rroxscaffold_4G00311670 Rroxscaffold_4G00311730 Rroxscaffold_4G00311750 Rroxscaffold_4G00312290 Rroxscaffold_6G00393430
rosa_rugosa Rorug01G0153800.1 Rorug01G0153900.1 Rorug01G0160100.1 Rorug03G0243800
rosa_samantha Rh1AG166400 Rh1AG168200 Rh1AG174100 Rh1AG174700 Rh1AG179000 Rh1BG132600 Rh1BG136500 Rh1BG142200 Rh1BG142800 Rh1BG143200 Rh1BG146200 Rh1BG147000 Rh1BG147100 Rh1CG154800 Rh1CG161900 Rh1CG162700 Rh1CG166000 Rh1DG167500 Rh1DG169900 Rh1DG174000 Rh1DG174400 Rh1DG174700 Rh1DG174800 Rh1DG178200 Rh1DG179000 Rh1DG179100 Rh3AG292800 Rh3BG329600 Rh3CG326900 Rh3DG326200 Rh5BG218300 Rh7CG326800 Rh7DG307100
rosa_wichuraiana Rw0G006190 Rw0G007830 Rw0G009970 Rw0G009980 Rw1G013880 Rw1G014000 Rw1G014500 Rw1G014560 Rw1G014920 Rw3G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 706
AccII CGCG 3 cut(s) 241, 665, 790
AciI CCGC 4 cut(s) 150, 239, 617, 702
AclWI GGATC 1 cut(s) 776
AcsI RAATTY 1 cut(s) 72
AcuI CTGAAG 1 cut(s) 695
AfaI GTAC 1 cut(s) 32
AfiI CCNNNNNNNGG 2 cut(s) 332, 732
AflIII ACRYGT 1 cut(s) 788
AgsI TTSAA 4 cut(s) 77, 220, 311, 797
AjnI CCWGG 2 cut(s) 328, 731
AluBI AGCT 4 cut(s) 66, 143, 410, 429
AluI AGCT 4 cut(s) 66, 143, 410, 429
Alw26I GTCTC 1 cut(s) 9
AlwI GGATC 1 cut(s) 776
ApeKI GCWGC 4 cut(s) 36, 152, 155, 686
ApoI RAATTY 1 cut(s) 72
AseI ATTAAT 1 cut(s) 101
AspLEI GCGC 3 cut(s) 622, 667, 811
AspS9I GGNCC 2 cut(s) 343, 723
AsuHPI GGTGA 3 cut(s) 162, 262, 502
AvaII GGWCC 2 cut(s) 343, 723
BbsI GAAGAC 1 cut(s) 115
BbvI GCAGC 4 cut(s) 48, 139, 142, 673
BccI CCATC 3 cut(s) 82, 268, 491
BceAI ACGGC 1 cut(s) 299
BciT130I CCWGG 2 cut(s) 330, 733
BciVI GTATCC 1 cut(s) 728
BcoDI GTCTC 1 cut(s) 9
BfaI CTAG 1 cut(s) 614
BfmI CTRYAG 2 cut(s) 417, 813
BfoI RGCGCY 1 cut(s) 623
BfuI GTATCC 1 cut(s) 728
BisI GCNGC 6 cut(s) 37, 150, 153, 156, 618, 687
BlsI GCNGC 6 cut(s) 38, 151, 154, 157, 619, 688
Bme1390I CCNGG 2 cut(s) 330, 733
Bme18I GGWCC 2 cut(s) 343, 723
BmgT120I GGNCC 2 cut(s) 343, 723
BmiI GGNNCC 1 cut(s) 344
BmrFI CCNGG 2 cut(s) 330, 733
BmsI GCATC 1 cut(s) 796
BpiI GAAGAC 1 cut(s) 115
BpmI CTGGAG 1 cut(s) 351
Bsa29I ATCGAT 1 cut(s) 780
BsaAI YACGTR 2 cut(s) 263, 776
BsaJI CCNNGG 1 cut(s) 367
BsaWI WCCGGW 1 cut(s) 720
BsaXI ACNNNNNCTCC 2 cut(s) 522, 552
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 732
Bse1I ACTGG 1 cut(s) 230
BseBI CCWGG 2 cut(s) 330, 733
BseCI ATCGAT 1 cut(s) 780
BseDI CCNNGG 1 cut(s) 367
BseLI CCNNNNNNNGG 2 cut(s) 332, 732
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 230
BseXI GCAGC 4 cut(s) 48, 139, 142, 673
Bsh1236I CGCG 3 cut(s) 241, 665, 790
BshVI ATCGAT 1 cut(s) 780
BsiSI CCGG 1 cut(s) 721
BslFI GGGAC 1 cut(s) 356
BslI CCNNNNNNNGG 2 cut(s) 332, 732
BsmAI GTCTC 1 cut(s) 9
BsmFI GGGAC 1 cut(s) 356
Bsp143I GATC 3 cut(s) 291, 759, 781
BspACI CCGC 4 cut(s) 150, 239, 617, 702
BspCNI CTCAG 1 cut(s) 152
BspDI ATCGAT 1 cut(s) 780
BspFNI CGCG 3 cut(s) 241, 665, 790
BspLI GGNNCC 1 cut(s) 344
BspMAI CTGCAG 2 cut(s) 421, 817
BspPI GGATC 1 cut(s) 776
BsrI ACTGG 1 cut(s) 230
BssECI CCNNGG 1 cut(s) 367
BssMI GATC 3 cut(s) 291, 759, 781
BssT1I CCWWGG 1 cut(s) 367
Bst2UI CCWGG 2 cut(s) 330, 733
Bst4CI ACNGT 1 cut(s) 437
Bst6I CTCTTC 1 cut(s) 792
BstBAI YACGTR 2 cut(s) 263, 776
BstC8I GCNNGC 3 cut(s) 269, 604, 813
BstDEI CTNAG 3 cut(s) 25, 67, 139
BstFNI CGCG 3 cut(s) 241, 665, 790
BstH2I RGCGCY 1 cut(s) 623
BstHHI GCGC 3 cut(s) 622, 667, 811
BstKTI GATC 3 cut(s) 294, 762, 784
BstMAI GTCTC 1 cut(s) 9
BstMBI GATC 3 cut(s) 291, 759, 781
BstMWI GCNNNNNNNGC 3 cut(s) 149, 155, 416
BstNI CCWGG 2 cut(s) 330, 733
BstNSI RCATGY 2 cut(s) 271, 606
BstSCI CCNGG 2 cut(s) 328, 731
BstSFI CTRYAG 2 cut(s) 417, 813
BstUI CGCG 3 cut(s) 241, 665, 790
BstV1I GCAGC 4 cut(s) 48, 139, 142, 673
BstV2I GAAGAC 1 cut(s) 115
Bsu15I ATCGAT 1 cut(s) 780
BsuI GTATCC 1 cut(s) 728
BsuTUI ATCGAT 1 cut(s) 780
BtsI GCAGTG 2 cut(s) 394, 426
BtsIMutI CAGTG 2 cut(s) 394, 426
Cac8I GCNNGC 3 cut(s) 269, 604, 813
CfoI GCGC 3 cut(s) 622, 667, 811
Cfr13I GGNCC 2 cut(s) 343, 723
ClaI ATCGAT 1 cut(s) 780
CpoI CGGWCCG 1 cut(s) 723
CseI GACGC 1 cut(s) 101
Csp6I GTAC 1 cut(s) 31
CspI CGGWCCG 1 cut(s) 723
CviAII CATG 5 cut(s) 13, 268, 301, 577, 603
CviJI RGCY 9 cut(s) 66, 143, 284, 314, 410, 429, 550, 562, 686
CviKI_1 RGCY 9 cut(s) 66, 143, 284, 314, 410, 429, 550, 562, 686
CviQI GTAC 1 cut(s) 31
DdeI CTNAG 3 cut(s) 25, 67, 139
DpnI GATC 3 cut(s) 293, 761, 783
DpnII GATC 3 cut(s) 291, 759, 781
Eam1104I CTCTTC 1 cut(s) 792
EarI CTCTTC 1 cut(s) 792
Eco130I CCWWGG 1 cut(s) 367
Eco32I GATATC 2 cut(s) 22, 236
Eco47I GGWCC 2 cut(s) 343, 723
Eco57I CTGAAG 1 cut(s) 695
EcoRII CCWGG 2 cut(s) 328, 731
EcoRV GATATC 2 cut(s) 22, 236
EcoT14I CCWWGG 1 cut(s) 367
ErhI CCWWGG 1 cut(s) 367
FaeI CATG 5 cut(s) 16, 271, 304, 580, 606
FaqI GGGAC 1 cut(s) 356
FatI CATG 5 cut(s) 12, 267, 300, 576, 602
FblI GTMKAC 1 cut(s) 706
Fnu4HI GCNGC 6 cut(s) 37, 150, 153, 156, 618, 687
Fsp4HI GCNGC 6 cut(s) 37, 150, 153, 156, 618, 687
FspBI CTAG 1 cut(s) 614
GlaI GCGC 3 cut(s) 621, 666, 810
GluI GCNGC 6 cut(s) 37, 150, 153, 156, 618, 687
GsuI CTGGAG 1 cut(s) 351
HaeII RGCGCY 1 cut(s) 623
HapII CCGG 1 cut(s) 721
HgaI GACGC 1 cut(s) 101
HhaI GCGC 3 cut(s) 622, 667, 811
Hin1II CATG 5 cut(s) 16, 271, 304, 580, 606
Hin6I GCGC 3 cut(s) 620, 665, 809
HinP1I GCGC 3 cut(s) 620, 665, 809
HincII GTYRAC 3 cut(s) 322, 454, 738
HindII GTYRAC 3 cut(s) 322, 454, 738
HindIII AAGCTT 1 cut(s) 64
HinfI GANTC 1 cut(s) 392
HpaI GTTAAC 1 cut(s) 738
HpaII CCGG 1 cut(s) 721
HphI GGTGA 3 cut(s) 162, 262, 502
Hpy166II GTNNAC 5 cut(s) 322, 454, 530, 707, 738
Hpy188I TCNGA 3 cut(s) 9, 19, 107
Hpy188III TCNNGA 3 cut(s) 208, 295, 396
Hpy8I GTNNAC 5 cut(s) 322, 454, 530, 707, 738
HpyAV CCTTC 2 cut(s) 177, 247
HpyCH4III ACNGT 1 cut(s) 437
HpyCH4IV ACGT 4 cut(s) 262, 450, 557, 775
HpyCH4V TGCA 5 cut(s) 44, 387, 419, 606, 815
HpyF10VI GCNNNNNNNGC 3 cut(s) 149, 155, 416
HpyF3I CTNAG 3 cut(s) 25, 67, 139
HpySE526I ACGT 4 cut(s) 262, 450, 557, 775
Hsp92II CATG 5 cut(s) 16, 271, 304, 580, 606
HspAI GCGC 3 cut(s) 620, 665, 809
KspAI GTTAAC 1 cut(s) 738
Kzo9I GATC 3 cut(s) 291, 759, 781
LpnPI CCDG 9 cut(s) 81, 211, 315, 342, 396, 698, 718, 734, 745
Lsp1109I GCAGC 4 cut(s) 48, 139, 142, 673
LweI GCATC 1 cut(s) 796
MaeI CTAG 1 cut(s) 614
MaeII ACGT 4 cut(s) 262, 450, 557, 775
MaeIII GTNAC 1 cut(s) 508
MalI GATC 3 cut(s) 293, 761, 783
MboI GATC 3 cut(s) 291, 759, 781
MboII GAAGA 4 cut(s) 120, 205, 232, 809
MluCI AATT 3 cut(s) 72, 215, 381
MluI ACGCGT 1 cut(s) 788
MmeI TCCRAC 1 cut(s) 809
MnlI CCTC 3 cut(s) 43, 326, 546
MseI TTAA 3 cut(s) 101, 425, 737
MslI CAYNNNNRTG 1 cut(s) 272
MspA1I CMGCKG 3 cut(s) 143, 152, 702
MspI CCGG 1 cut(s) 721
MspR9I CCNGG 2 cut(s) 330, 733
MvaI CCWGG 2 cut(s) 330, 733
MvnI CGCG 3 cut(s) 241, 665, 790
MwoI GCNNNNNNNGC 3 cut(s) 149, 155, 416
NdeII GATC 3 cut(s) 291, 759, 781
NlaIII CATG 5 cut(s) 16, 271, 304, 580, 606
NlaIV GGNNCC 1 cut(s) 344
NmuCI GTSAC 1 cut(s) 508
NspI RCATGY 2 cut(s) 271, 606
PaeI GCATGC 2 cut(s) 271, 606
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PfeI GAWTC 1 cut(s) 392
PflFI GACNNNGTC 1 cut(s) 13
PkrI GCNGC 6 cut(s) 38, 151, 154, 157, 619, 688
Ppu21I YACGTR 2 cut(s) 263, 776
PshBI ATTAAT 1 cut(s) 101
Psp6I CCWGG 2 cut(s) 328, 731
PspGI CCWGG 2 cut(s) 328, 731
PspN4I GGNNCC 1 cut(s) 344
PspPI GGNCC 2 cut(s) 343, 723
PstI CTGCAG 2 cut(s) 421, 817
PsyI GACNNNGTC 1 cut(s) 13
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 1 cut(s) 32
RsaNI GTAC 1 cut(s) 31
RseI CAYNNNNRTG 1 cut(s) 272
Rsr2I CGGWCCG 1 cut(s) 723
RsrII CGGWCCG 1 cut(s) 723
SaqAI TTAA 3 cut(s) 101, 425, 737
SatI GCNGC 6 cut(s) 37, 150, 153, 156, 618, 687
Sau3AI GATC 3 cut(s) 291, 759, 781
Sau96I GGNCC 2 cut(s) 343, 723
ScrFI CCNGG 2 cut(s) 330, 733
SfaNI GCATC 1 cut(s) 796
SfcI CTRYAG 2 cut(s) 417, 813
SinI GGWCC 2 cut(s) 343, 723
SmiMI CAYNNNNRTG 1 cut(s) 272
SphI GCATGC 2 cut(s) 271, 606
Sse9I AATT 3 cut(s) 72, 215, 381
SsiI CCGC 4 cut(s) 150, 239, 617, 702
SspMI CTAG 1 cut(s) 614
StyD4I CCNGG 2 cut(s) 328, 731
StyI CCWWGG 1 cut(s) 367
TaaI ACNGT 1 cut(s) 437
TaiI ACGT 4 cut(s) 265, 453, 560, 778
TaqI TCGA 4 cut(s) 296, 395, 633, 780
TasI AATT 3 cut(s) 72, 215, 381
TatI WGTACW 1 cut(s) 30
TauI GCSGC 2 cut(s) 152, 620
TfiI GAWTC 1 cut(s) 392
Tru1I TTAA 3 cut(s) 101, 425, 737
Tru9I TTAA 3 cut(s) 101, 425, 737
TscAI CASTG 2 cut(s) 394, 426
TseFI GTSAC 1 cut(s) 508
TseI GCWGC 4 cut(s) 36, 152, 155, 686
Tsp45I GTSAC 1 cut(s) 508
TspDTI ATGAA 3 cut(s) 135, 457, 565
TspGWI ACGGA 1 cut(s) 715
TspRI CASTG 2 cut(s) 394, 426
Tth111I GACNNNGTC 1 cut(s) 13
VpaK11BI GGWCC 2 cut(s) 343, 723
VspI ATTAAT 1 cut(s) 101
XapI RAATTY 1 cut(s) 72
XceI RCATGY 2 cut(s) 271, 606
XmiI GTMKAC 1 cut(s) 706
XspI CTAG 1 cut(s) 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.