MD12G1214100.v1.1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0000404
Physical Location & Seq
Forward (+)
29257823 .. 29266468
8646 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1214100.v1.1.491

Sequence Viewer

Length: 1203 bp
ATGAGAGGAGGAGTTGAGGTGCTCCAGAAGTTGATTGGTAAAAATCCTGAGTCCATTTATGACAAAGTTAAAAACAGATCGAAAGAAACAGTTTTGCACTTGTGTATTATACACAACCAGTTCGAGTGCTTGAAACTGTTGGTTGATAGACTGTTGGTTGAAAGAGACGACAGAAATGGTGAGTTCCTCAACTCAAGAGCTGGCTGTGATGGTGGTGTGACCATCCTGCACTTAGCTTTGATGCTAAGGCAAATTAAGACTATACGTTACCTGCTTTCCGTTGATGCTATAAGAGCAGAAGCAACTGGTGTGAATGGGATGTCTCTGACCATGTTAGATATCTTAGAGTACAGCAGCGTTGCAAGAGAGGACTTTAGCAGAAGCTTAGAAATTCAACAGATTTTGATGGACGCAGGATTAATCAGAAGACAAAATAATGAAAATGATAAGCCTAACTCAGATGTTGCCGCTGCTGCTGTTGTATCACCAAATCCAAAAAGGGTAACGAAGGAAAAGAAGCTTCACAAACGAGTAGCATCATCGGAAAAGGGATCAACGCCAGCAAGGAGGTGCTGGACAAAATTGATGGAATGGTTGAGATATCCGAGTAATTGGGTGGGCGAGACACGTGGCATGCTGATGGTTGTGGCTACGATGATCTCGACCATGACTTTTCAAGCCGTAGTCAACCCACCTGGTGGTGTCTGGGACCATAATGATACAAACACTACCATTTATTATTCTGGAACCAATTATACCAGAACAATTTGCAGTGAAACGAGAGTATGTAGAGCTGGAACTGCAGTGTTAAGCTACGGTAATGATGACGACTTCGCCGATTTCCTAACATCCAATACCATCTCGTTCCTTGCTTCTTTGAGTGTCACCCTTTTGCTCGTTAGTGGATTTCCTCTCCACAATCGGTTCTGCACGTGGCTCTTATCGATGTCCATGTGCGTCACTCTCACATTCCTGGCATTCACCTACCTAGTTGCGCTGTATATGATCGTCCATGGTTCACCTACAGATACAAGCATTAACCTATACGGGATATCCGTTCTTACTTGGACTACTTTGCTGGGCATGGTCAGTGTAATGCACACTATTCGGTTTCTTATTCGGGTGACGAAGTGGTTGCGGCGCATGTTCTTCAGGTCAAAATCATGTTCTTGTTTGTGTAATTACTCTGCCATGGTGTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

401

Amino Acids

45.04

Weight (kDa)

9.1

Isoelectric Point (pI)

34.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 4 - 93 2.7e-09 Ankyrin repeats (3 copies)
PGG PF13962 204 - 335 1.1e-17 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13950 AT4G13266
fragaria_vesca FvH4_7g06240 FvH4_7g06250 FvH4_7g06270
malus_domestica MD12G1213300.v1.1 MD12G1213900.v1.1 MD12G1214100.v1.1 MD12G1214700.v1.1 MD12G1214800.v1.1
prunus_persica Prupe.2G107700_v2.0.a1 Prupe.3G142200_v2.0.a1 Prupe.3G154100_v2.0.a1 Prupe.3G154300_v2.0.a1 Prupe.3G154900_v2.0.a1 Prupe.3G155100_v2.0.a1
pyrus_communis pycom12g20010 pycom12g20080 pycom12g20090 pycom12g20100
rosa_chinensis RchiOBHm_Chr1g0341001 RchiOBHm_Chr1g0341021 RchiOBHm_Chr1g0341891 RchiOBHm_Chr1g0342341 RchiOBHm_Chr3g0490431
rosa_laevigata RLG00000022889 RLG00000029016 RLG00000029039 RLG00000029059 RLG00000029061 RLG00000029065 RLG00000029115 RLG00000029155 RLG00000033311 RLG00000033312
rosa_multiflora Rmu_sc0000404.1_g000006 Rmu_sc0001836.1_g000015 Rmu_sc0001836.1_g000050 Rmu_sc0002200.1_g000003 Rmu_sc0003862.1_g000020 Rmu_sc0005193.1_g000001 Rmu_sc0005193.1_g000002 Rmu_sc0008453.1_g000008 Rmu_sc0008453.1_g000009 Rmu_sc0008453.1_g000010 Rmu_sc0008862.1_g000001 Rmu_sc0011195.1_g000003 Rmu_sc0014077.1_g000001
rosa_roxburghii Rroxscaffold_4G00311670 Rroxscaffold_4G00311730 Rroxscaffold_4G00311750 Rroxscaffold_4G00312290 Rroxscaffold_6G00393430
rosa_rugosa Rorug01G0153800.1 Rorug01G0153900.1 Rorug01G0160100.1 Rorug03G0243800
rosa_samantha Rh1AG166400 Rh1AG168200 Rh1AG174100 Rh1AG174700 Rh1AG179000 Rh1BG132600 Rh1BG136500 Rh1BG142200 Rh1BG142800 Rh1BG143200 Rh1BG146200 Rh1BG147000 Rh1BG147100 Rh1CG154800 Rh1CG161900 Rh1CG162700 Rh1CG166000 Rh1DG167500 Rh1DG169900 Rh1DG174000 Rh1DG174400 Rh1DG174700 Rh1DG174800 Rh1DG178200 Rh1DG179000 Rh1DG179100 Rh3AG292800 Rh3BG329600 Rh3CG326900 Rh3DG326200 Rh5BG218300 Rh7CG326800 Rh7DG307100
rosa_wichuraiana Rw0G006190 Rw0G007830 Rw0G009970 Rw0G009980 Rw1G013880 Rw1G014000 Rw1G014500 Rw1G014560 Rw1G014920 Rw3G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 279
AccB7I CCANNNNNTGG 1 cut(s) 698
AciI CCGC 2 cut(s) 468, 1138
AclWI GGATC 1 cut(s) 559
AcsI RAATTY 1 cut(s) 390
AcuI CTGAAG 1 cut(s) 1135
AcvI CACGTG 2 cut(s) 629, 933
AdeI CACNNNGTG 1 cut(s) 698
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 1 cut(s) 698
AflIII ACRYGT 1 cut(s) 626
AgsI TTSAA 4 cut(s) 133, 161, 395, 677
AjnI CCWGG 2 cut(s) 694, 972
AluBI AGCT 6 cut(s) 200, 236, 384, 520, 794, 813
AluI AGCT 6 cut(s) 200, 236, 384, 520, 794, 813
Alw21I GWGCWC 1 cut(s) 24
Alw26I GTCTC 3 cut(s) 159, 327, 617
AlwI GGATC 1 cut(s) 559
ApeKI GCWGC 3 cut(s) 354, 470, 473
ApoI RAATTY 1 cut(s) 390
AseI ATTAAT 1 cut(s) 419
AspLEI GCGC 2 cut(s) 997, 1143
AspS9I GGNCC 1 cut(s) 709
AsuHPI GGTGA 6 cut(s) 191, 477, 877, 973, 1011, 1135
AvaII GGWCC 1 cut(s) 709
BbrPI CACGTG 2 cut(s) 629, 933
BbsI GAAGAC 1 cut(s) 433
Bbv12I GWGCWC 1 cut(s) 24
BbvI GCAGC 3 cut(s) 366, 457, 460
BccI CCATC 6 cut(s) 203, 230, 400, 580, 634, 866
BceAI ACGGC 1 cut(s) 665
BcgI CGANNNNNNTGC 4 cut(s) 1088, 1117, 1122, 1151
BciT130I CCWGG 2 cut(s) 696, 974
BcoDI GTCTC 3 cut(s) 159, 327, 617
BfaI CTAG 1 cut(s) 989
BfmI CTRYAG 2 cut(s) 801, 1023
BfuAI ACCTGC 1 cut(s) 279
BisI GCNGC 5 cut(s) 355, 468, 471, 474, 1139
BlsI GCNGC 5 cut(s) 356, 469, 472, 475, 1140
Bme1390I CCNGG 2 cut(s) 696, 974
Bme18I GGWCC 1 cut(s) 709
BmgT120I GGNCC 1 cut(s) 709
BmiI GGNNCC 2 cut(s) 710, 748
BmrFI CCNGG 2 cut(s) 696, 974
BmsI GCATC 3 cut(s) 231, 274, 545
BpiI GAAGAC 1 cut(s) 433
BpmI CTGGAG 1 cut(s) 8
Bpu10I CCTNAGC 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 178
Bsa29I ATCGAT 1 cut(s) 944
BsaAI YACGTR 2 cut(s) 629, 933
BsaJI CCNNGG 2 cut(s) 1012, 1191
BsaXI ACNNNNNCTCC 1 cut(s) 33
Bsc4I CCNNNNNNNGG 1 cut(s) 698
Bse1I ACTGG 2 cut(s) 118, 310
BseBI CCWGG 2 cut(s) 696, 974
BseCI ATCGAT 1 cut(s) 944
BseDI CCNNGG 2 cut(s) 1012, 1191
BseGI GGATG 3 cut(s) 222, 324, 848
BseLI CCNNNNNNNGG 1 cut(s) 698
BseMII CTCAG 2 cut(s) 39, 471
BseNI ACTGG 2 cut(s) 118, 310
BseRI GAGGAG 2 cut(s) 21, 24
BseXI GCAGC 3 cut(s) 366, 457, 460
BseYI CCCAGC 1 cut(s) 1078
BsgI GTGCAG 2 cut(s) 212, 913
BshVI ATCGAT 1 cut(s) 944
BsiHKAI GWGCWC 1 cut(s) 24
BslFI GGGAC 1 cut(s) 722
BslI CCNNNNNNNGG 1 cut(s) 698
BsmAI GTCTC 3 cut(s) 159, 327, 617
BsmBI CGTCTC 1 cut(s) 159
BsmFI GGGAC 1 cut(s) 722
BsmI GAATGC 1 cut(s) 977
Bsp1286I GDGCHC 1 cut(s) 24
Bsp143I GATC 4 cut(s) 77, 551, 657, 1005
Bsp19I CCATGG 2 cut(s) 1012, 1191
BspACI CCGC 2 cut(s) 468, 1138
BspCNI CTCAG 2 cut(s) 40, 470
BspDI ATCGAT 1 cut(s) 944
BspLI GGNNCC 2 cut(s) 710, 748
BspMAI CTGCAG 1 cut(s) 805
BspMI ACCTGC 1 cut(s) 279
BspPI GGATC 1 cut(s) 559
BsrI ACTGG 2 cut(s) 118, 310
BssECI CCNNGG 2 cut(s) 1012, 1191
BssMI GATC 4 cut(s) 77, 551, 657, 1005
BssT1I CCWWGG 2 cut(s) 1012, 1191
Bst2UI CCWGG 2 cut(s) 696, 974
Bst4CI ACNGT 4 cut(s) 91, 138, 153, 818
BstBAI YACGTR 2 cut(s) 629, 933
BstC8I GCNNGC 3 cut(s) 202, 561, 635
BstDEI CTNAG 6 cut(s) 48, 232, 245, 343, 385, 457
BstDSI CCRYGG 2 cut(s) 1012, 1191
BstF5I GGATG 3 cut(s) 222, 324, 848
BstHHI GCGC 2 cut(s) 997, 1143
BstKTI GATC 4 cut(s) 80, 554, 660, 1008
BstMAI GTCTC 3 cut(s) 159, 327, 617
BstMBI GATC 4 cut(s) 77, 551, 657, 1005
BstMWI GCNNNNNNNGC 3 cut(s) 293, 473, 800
BstNI CCWGG 2 cut(s) 696, 974
BstNSI RCATGY 2 cut(s) 637, 1147
BstSCI CCNGG 2 cut(s) 694, 972
BstSFI CTRYAG 2 cut(s) 801, 1023
BstV1I GCAGC 3 cut(s) 366, 457, 460
BstV2I GAAGAC 1 cut(s) 433
Bsu15I ATCGAT 1 cut(s) 944
BsuTUI ATCGAT 1 cut(s) 944
BtgI CCRYGG 2 cut(s) 1012, 1191
BtsCI GGATG 3 cut(s) 222, 324, 848
BtsI GCAGTG 2 cut(s) 778, 810
BtsIMutI CAGTG 3 cut(s) 778, 810, 1096
BveI ACCTGC 1 cut(s) 279
Cac8I GCNNGC 3 cut(s) 202, 561, 635
CfoI GCGC 2 cut(s) 997, 1143
Cfr13I GGNCC 1 cut(s) 709
ClaI ATCGAT 1 cut(s) 944
CseI GACGC 2 cut(s) 419, 946
CsiI ACCWGGT 1 cut(s) 694
Csp6I GTAC 1 cut(s) 349
CviAII CATG 9 cut(s) 331, 634, 667, 952, 1013, 1084, 1144, 1164, 1192
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 6 cut(s) 48, 232, 245, 343, 385, 457
DpnI GATC 4 cut(s) 79, 553, 659, 1007
DpnII GATC 4 cut(s) 77, 551, 657, 1005
DraIII CACNNNGTG 1 cut(s) 698
Eco130I CCWWGG 2 cut(s) 1012, 1191
Eco32I GATATC 3 cut(s) 340, 602, 1053
Eco47I GGWCC 1 cut(s) 709
Eco57I CTGAAG 1 cut(s) 1135
Eco72I CACGTG 2 cut(s) 629, 933
EcoRII CCWGG 2 cut(s) 694, 972
EcoRV GATATC 3 cut(s) 340, 602, 1053
EcoT14I CCWWGG 2 cut(s) 1012, 1191
ErhI CCWWGG 2 cut(s) 1012, 1191
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 9 cut(s) 334, 637, 670, 955, 1016, 1087, 1147, 1167, 1195
FaqI GGGAC 1 cut(s) 722
FatI CATG 9 cut(s) 330, 633, 666, 951, 1012, 1083, 1143, 1163, 1191
Fnu4HI GCNGC 5 cut(s) 355, 468, 471, 474, 1139
FokI GGATG 3 cut(s) 209, 331, 835
Fsp4HI GCNGC 5 cut(s) 355, 468, 471, 474, 1139
FspBI CTAG 1 cut(s) 989
GlaI GCGC 2 cut(s) 996, 1142
GluI GCNGC 5 cut(s) 355, 468, 471, 474, 1139
GsaI CCCAGC 1 cut(s) 1082
GsuI CTGGAG 1 cut(s) 8
HgaI GACGC 2 cut(s) 419, 946
HhaI GCGC 2 cut(s) 997, 1143
Hin1II CATG 9 cut(s) 334, 637, 670, 955, 1016, 1087, 1147, 1167, 1195
Hin6I GCGC 2 cut(s) 995, 1141
HinP1I GCGC 2 cut(s) 995, 1141
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HindIII AAGCTT 2 cut(s) 382, 518
HinfI GANTC 1 cut(s) 50
HphI GGTGA 6 cut(s) 191, 477, 877, 973, 1011, 1135
Hpy166II GTNNAC 2 cut(s) 688, 1019
Hpy188I TCNGA 5 cut(s) 327, 425, 460, 544, 606
Hpy188III TCNNGA 5 cut(s) 25, 47, 195, 661, 744
Hpy8I GTNNAC 2 cut(s) 688, 1019
HpyAV CCTTC 1 cut(s) 502
HpyCH4III ACNGT 4 cut(s) 91, 138, 153, 818
HpyCH4IV ACGT 3 cut(s) 265, 628, 932
HpyCH4V TGCA 7 cut(s) 97, 229, 362, 771, 803, 930, 1099
HpyF10VI GCNNNNNNNGC 3 cut(s) 293, 473, 800
HpyF3I CTNAG 6 cut(s) 48, 232, 245, 343, 385, 457
HpySE526I ACGT 3 cut(s) 265, 628, 932
Hsp92II CATG 9 cut(s) 334, 637, 670, 955, 1016, 1087, 1147, 1167, 1195
HspAI GCGC 2 cut(s) 995, 1141
Kzo9I GATC 4 cut(s) 77, 551, 657, 1005
LmnI GCTCC 1 cut(s) 27
Lsp1109I GCAGC 3 cut(s) 366, 457, 460
LweI GCATC 3 cut(s) 231, 274, 545
MabI ACCWGGT 1 cut(s) 694
MaeI CTAG 1 cut(s) 989
MaeII ACGT 3 cut(s) 265, 628, 932
MaeIII GTNAC 6 cut(s) 217, 266, 502, 883, 958, 1123
MalI GATC 4 cut(s) 79, 553, 659, 1007
MboI GATC 4 cut(s) 77, 551, 657, 1005
MboII GAAGA 2 cut(s) 438, 1141
MhlI GDGCHC 1 cut(s) 24
MluCI AATT 7 cut(s) 252, 390, 581, 610, 751, 765, 1180
MlyI GAGTC 1 cut(s) 59
MnlI CCTC 5 cut(s) 10, 197, 361, 561, 921
MseI TTAA 5 cut(s) 69, 255, 419, 809, 1038
MslI CAYNNNNRTG 1 cut(s) 638
MspA1I CMGCKG 1 cut(s) 470
MspR9I CCNGG 2 cut(s) 696, 974
Mva1269I GAATGC 1 cut(s) 977
MvaI CCWGG 2 cut(s) 696, 974
MwoI GCNNNNNNNGC 3 cut(s) 293, 473, 800
NcoI CCATGG 2 cut(s) 1012, 1191
NdeII GATC 4 cut(s) 77, 551, 657, 1005
NlaIII CATG 9 cut(s) 334, 637, 670, 955, 1016, 1087, 1147, 1167, 1195
NlaIV GGNNCC 2 cut(s) 710, 748
NmuCI GTSAC 4 cut(s) 217, 883, 958, 1123
NspI RCATGY 2 cut(s) 637, 1147
PaeI GCATGC 1 cut(s) 637
PcsI WCGNNNNNNNCGW 3 cut(s) 659, 834, 1053
PctI GAATGC 1 cut(s) 977
PflMI CCANNNNNTGG 1 cut(s) 698
PkrI GCNGC 5 cut(s) 356, 469, 472, 475, 1140
PleI GAGTC 1 cut(s) 58
PmaCI CACGTG 2 cut(s) 629, 933
PmlI CACGTG 2 cut(s) 629, 933
PpsI GAGTC 1 cut(s) 58
Ppu21I YACGTR 2 cut(s) 629, 933
PshBI ATTAAT 1 cut(s) 419
Psp6I CCWGG 2 cut(s) 694, 972
PspCI CACGTG 2 cut(s) 629, 933
PspFI CCCAGC 1 cut(s) 1078
PspGI CCWGG 2 cut(s) 694, 972
PspN4I GGNNCC 2 cut(s) 710, 748
PspPI GGNCC 1 cut(s) 709
PsrI GAACNNNNNNTAC 2 cut(s) 739, 771
PstI CTGCAG 1 cut(s) 805
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
RseI CAYNNNNRTG 1 cut(s) 638
SaqAI TTAA 5 cut(s) 69, 255, 419, 809, 1038
SatI GCNGC 5 cut(s) 355, 468, 471, 474, 1139
Sau3AI GATC 4 cut(s) 77, 551, 657, 1005
Sau96I GGNCC 1 cut(s) 709
SchI GAGTC 1 cut(s) 59
ScrFI CCNGG 2 cut(s) 696, 974
SduI GDGCHC 1 cut(s) 24
SexAI ACCWGGT 1 cut(s) 694
SfaNI GCATC 3 cut(s) 231, 274, 545
SfcI CTRYAG 2 cut(s) 801, 1023
SinI GGWCC 1 cut(s) 709
SmiMI CAYNNNNRTG 1 cut(s) 638
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
SphI GCATGC 1 cut(s) 637
Sse9I AATT 7 cut(s) 252, 390, 581, 610, 751, 765, 1180
SsiI CCGC 2 cut(s) 468, 1138
SspMI CTAG 1 cut(s) 989
StyD4I CCNGG 2 cut(s) 694, 972
StyI CCWWGG 2 cut(s) 1012, 1191
TaaI ACNGT 4 cut(s) 91, 138, 153, 818
TaiI ACGT 3 cut(s) 268, 631, 935
TaqI TCGA 4 cut(s) 80, 123, 662, 944
TasI AATT 7 cut(s) 252, 390, 581, 610, 751, 765, 1180
TatI WGTACW 1 cut(s) 348
TauI GCSGC 2 cut(s) 470, 1141
Tru1I TTAA 5 cut(s) 69, 255, 419, 809, 1038
Tru9I TTAA 5 cut(s) 69, 255, 419, 809, 1038
TscAI CASTG 3 cut(s) 778, 810, 1096
TseFI GTSAC 4 cut(s) 217, 883, 958, 1123
TseI GCWGC 3 cut(s) 354, 470, 473
Tsp45I GTSAC 4 cut(s) 217, 883, 958, 1123
TspDTI ATGAA 1 cut(s) 453
TspGWI ACGGA 2 cut(s) 268, 1045
TspRI CASTG 3 cut(s) 778, 810, 1096
Van91I CCANNNNNTGG 1 cut(s) 698
VpaK11BI GGWCC 1 cut(s) 709
VspI ATTAAT 1 cut(s) 419
XapI RAATTY 1 cut(s) 390
XceI RCATGY 2 cut(s) 637, 1147
XcmI CCANNNNNNNNNTGG 1 cut(s) 32
XspI CTAG 1 cut(s) 989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.