RLG00000033312

Ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000404
Physical Location & Seq
Reverse (-)
25591427 .. 25595766
4340 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033312

Sequence Viewer

Length: 684 bp
ATGGAGAACCAAGAAGAGCAAGCTCGGATTCAAGCTCGAACACTCCAAGCACCGGCTCGGGATCGAGAAGAGGAGAACGAGATTAGAGGAGAAATTGGGCAAGACACACCACATCTCATGAAGGAGCTTATTGAGAGCATGGATCGCTTGGTTTCCCAGCTAGAGGTATCAAGTGACCTCAAGGAAAAAGTCCAGCTATTGATGAAGACTGGCATCATTAGCAACATTGCTCAGCCCAGTGTTGAAGCTCAAGAAAGCAGGATTCCAGTGGAGGAGGAATCAAACTGGGTGGAGAGTCAAAGGGCAATACTCATTGGCGTGGCTGCCACGATCGCATTGATGACAACCACATATATGGTTAGCCCACCTGGTAGGGTTTGGCAGAATGCAGGCAATGACCCAACATTTTGCACTCCTTTGAGCCCTTGCATCTCTCATGTGCAGCCAGCACTTTTCCAGGAATTCATCAAGAACAATTGTATATGCTTCGCTTTCTCCATGAGCGTCATTCTCCTTTTGTTCTGTGGATTTCGGTCACACAAGAAAGCGACCGTGTGGATATACAAAGTGCTCATGTGCCTCATATTATTCTTCTGGAGCATGGCCTTCTACAAGGCCGTCAGCTTGGTAGATTCGAGCTCAGCTGTACCTCCTACCAGCATAGACTCCAGGTCATTGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

228

Amino Acids

25.53

Weight (kDa)

5.1

Isoelectric Point (pI)

60.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 95 - 209 1.8e-09 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13950 AT4G13266
fragaria_vesca FvH4_7g06240 FvH4_7g06250 FvH4_7g06270
malus_domestica MD12G1213300.v1.1 MD12G1213900.v1.1 MD12G1214100.v1.1 MD12G1214700.v1.1 MD12G1214800.v1.1
prunus_persica Prupe.2G107700_v2.0.a1 Prupe.3G142200_v2.0.a1 Prupe.3G154100_v2.0.a1 Prupe.3G154300_v2.0.a1 Prupe.3G154900_v2.0.a1 Prupe.3G155100_v2.0.a1
pyrus_communis pycom12g20010 pycom12g20080 pycom12g20090 pycom12g20100
rosa_chinensis RchiOBHm_Chr1g0341001 RchiOBHm_Chr1g0341021 RchiOBHm_Chr1g0341891 RchiOBHm_Chr1g0342341 RchiOBHm_Chr3g0490431
rosa_laevigata RLG00000022889 RLG00000029016 RLG00000029039 RLG00000029059 RLG00000029061 RLG00000029065 RLG00000029115 RLG00000029155 RLG00000033311 RLG00000033312
rosa_multiflora Rmu_sc0000404.1_g000006 Rmu_sc0001836.1_g000015 Rmu_sc0001836.1_g000050 Rmu_sc0002200.1_g000003 Rmu_sc0003862.1_g000020 Rmu_sc0005193.1_g000001 Rmu_sc0005193.1_g000002 Rmu_sc0008453.1_g000008 Rmu_sc0008453.1_g000009 Rmu_sc0008453.1_g000010 Rmu_sc0008862.1_g000001 Rmu_sc0011195.1_g000003 Rmu_sc0014077.1_g000001
rosa_roxburghii Rroxscaffold_4G00311670 Rroxscaffold_4G00311730 Rroxscaffold_4G00311750 Rroxscaffold_4G00312290 Rroxscaffold_6G00393430
rosa_rugosa Rorug01G0153800.1 Rorug01G0153900.1 Rorug01G0160100.1 Rorug03G0243800
rosa_samantha Rh1AG166400 Rh1AG168200 Rh1AG174100 Rh1AG174700 Rh1AG179000 Rh1BG132600 Rh1BG136500 Rh1BG142200 Rh1BG142800 Rh1BG143200 Rh1BG146200 Rh1BG147000 Rh1BG147100 Rh1CG154800 Rh1CG161900 Rh1CG162700 Rh1CG166000 Rh1DG167500 Rh1DG169900 Rh1DG174000 Rh1DG174400 Rh1DG174700 Rh1DG174800 Rh1DG178200 Rh1DG179000 Rh1DG179100 Rh3AG292800 Rh3BG329600 Rh3CG326900 Rh3DG326200 Rh5BG218300 Rh7CG326800 Rh7DG307100
rosa_wichuraiana Rw0G006190 Rw0G007830 Rw0G009970 Rw0G009980 Rw1G013880 Rw1G014000 Rw1G014500 Rw1G014560 Rw1G014920 Rw3G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 69, 150
AcsI RAATTY 1 cut(s) 461
AfaI GTAC 1 cut(s) 648
AfiI CCNNNNNNNGG 2 cut(s) 52, 163
AgsI TTSAA 2 cut(s) 32, 245
AhdI GACNNNNNGTC 1 cut(s) 670
AjnI CCWGG 3 cut(s) 367, 456, 668
AluBI AGCT 9 cut(s) 23, 35, 127, 160, 196, 248, 624, 639, 644
AluI AGCT 9 cut(s) 23, 35, 127, 160, 196, 248, 624, 639, 644
Alw21I GWGCWC 2 cut(s) 573, 641
AlwI GGATC 2 cut(s) 69, 150
Ama87I CYCGRG 1 cut(s) 57
AoxI GGCC 2 cut(s) 603, 615
ApeKI GCWGC 2 cut(s) 323, 442
ApoI RAATTY 1 cut(s) 461
AvaI CYCGRG 1 cut(s) 57
BanII GRGCYC 2 cut(s) 425, 641
BbsI GAAGAC 1 cut(s) 212
Bbv12I GWGCWC 2 cut(s) 573, 641
BbvI GCAGC 2 cut(s) 310, 454
BceAI ACGGC 1 cut(s) 602
BciT130I CCWGG 3 cut(s) 369, 458, 670
BfaI CTAG 1 cut(s) 161
BisI GCNGC 2 cut(s) 324, 443
BlpI GCTNAGC 2 cut(s) 231, 640
BlsI GCNGC 2 cut(s) 325, 444
Bme1390I CCNGG 3 cut(s) 369, 458, 670
BmeRI GACNNNNNGTC 1 cut(s) 670
BmeT110I CYCGRG 1 cut(s) 57
BmrFI CCNGG 3 cut(s) 369, 458, 670
BmrI ACTGGG 2 cut(s) 231, 295
BmsI GCATC 2 cut(s) 222, 438
BmuI ACTGGG 2 cut(s) 231, 295
BpiI GAAGAC 1 cut(s) 212
BpmI CTGGAG 2 cut(s) 616, 652
Bpu1102I GCTNAGC 2 cut(s) 231, 640
BpuEI CTTGAG 2 cut(s) 164, 234
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 163
Bse118I RCCGGY 1 cut(s) 52
Bse1I ACTGG 4 cut(s) 214, 237, 266, 290
Bse3DI GCAATG 2 cut(s) 225, 400
BseBI CCWGG 3 cut(s) 369, 458, 670
BseLI CCNNNNNNNGG 2 cut(s) 52, 163
BseMI GCAATG 2 cut(s) 225, 400
BseMII CTCAG 2 cut(s) 245, 654
BseNI ACTGG 4 cut(s) 214, 237, 266, 290
BseRI GAGGAG 3 cut(s) 86, 102, 287
BseXI GCAGC 2 cut(s) 310, 454
BseYI CCCAGC 1 cut(s) 156
BsgI GTGCAG 1 cut(s) 461
Bsh1285I CGRYCG 2 cut(s) 333, 552
BshFI GGCC 2 cut(s) 605, 617
BsiEI CGRYCG 2 cut(s) 333, 552
BsiHKAI GWGCWC 2 cut(s) 573, 641
BsiHKCI CYCGRG 1 cut(s) 57
BsiSI CCGG 1 cut(s) 53
BslI CCNNNNNNNGG 2 cut(s) 52, 163
BsmI GAATGC 1 cut(s) 391
BsnI GGCC 2 cut(s) 605, 617
BsoBI CYCGRG 1 cut(s) 57
Bsp1286I GDGCHC 3 cut(s) 425, 573, 641
Bsp143I GATC 3 cut(s) 61, 142, 330
Bsp1720I GCTNAGC 2 cut(s) 231, 640
BspANI GGCC 2 cut(s) 605, 617
BspCNI CTCAG 2 cut(s) 244, 653
BspHI TCATGA 1 cut(s) 117
BspPI GGATC 2 cut(s) 69, 150
BspQI GCTCTTC 1 cut(s) 9
BsrDI GCAATG 2 cut(s) 225, 400
BsrFI RCCGGY 1 cut(s) 52
BsrI ACTGG 4 cut(s) 214, 237, 266, 290
BssAI RCCGGY 1 cut(s) 52
BssMI GATC 3 cut(s) 61, 142, 330
Bst2UI CCWGG 3 cut(s) 369, 458, 670
Bst4CI ACNGT 1 cut(s) 553
Bst6I CTCTTC 2 cut(s) 9, 63
BstC8I GCNNGC 3 cut(s) 21, 391, 447
BstDEI CTNAG 2 cut(s) 231, 640
BstKTI GATC 3 cut(s) 64, 145, 333
BstMBI GATC 3 cut(s) 61, 142, 330
BstMCI CGRYCG 2 cut(s) 333, 552
BstMWI GCNNNNNNNGC 3 cut(s) 144, 219, 332
BstNI CCWGG 3 cut(s) 369, 458, 670
BstSCI CCNGG 3 cut(s) 367, 456, 668
BstV1I GCAGC 2 cut(s) 310, 454
BstV2I GAAGAC 1 cut(s) 212
BstXI CCANNNNNNTGG 2 cut(s) 355, 676
BsuRI GGCC 2 cut(s) 605, 617
BtsIMutI CAGTG 2 cut(s) 244, 273
Cac8I GCNNGC 3 cut(s) 21, 391, 447
CciI TCATGA 1 cut(s) 117
Cfr10I RCCGGY 1 cut(s) 52
CseI GACGC 1 cut(s) 493
CsiI ACCWGGT 1 cut(s) 367
Csp6I GTAC 1 cut(s) 647
CspCI CAANNNNNGTGG 2 cut(s) 270, 305
CviAII CATG 6 cut(s) 118, 139, 437, 499, 574, 601
CviQI GTAC 1 cut(s) 647
DdeI CTNAG 2 cut(s) 231, 640
DpnI GATC 3 cut(s) 63, 144, 332
DpnII GATC 3 cut(s) 61, 142, 330
DriI GACNNNNNGTC 1 cut(s) 670
Eam1104I CTCTTC 2 cut(s) 9, 63
Eam1105I GACNNNNNGTC 1 cut(s) 670
EarI CTCTTC 2 cut(s) 9, 63
Ecl136II GAGCTC 1 cut(s) 639
Eco24I GRGCYC 2 cut(s) 425, 641
Eco53kI GAGCTC 1 cut(s) 639
Eco88I CYCGRG 1 cut(s) 57
EcoICRI GAGCTC 1 cut(s) 639
EcoRI GAATTC 1 cut(s) 461
EcoRII CCWGG 3 cut(s) 367, 456, 668
EcoT38I GRGCYC 2 cut(s) 425, 641
FaeI CATG 6 cut(s) 121, 142, 440, 502, 577, 604
FatI CATG 6 cut(s) 117, 138, 436, 498, 573, 600
Fnu4HI GCNGC 2 cut(s) 324, 443
FriOI GRGCYC 2 cut(s) 425, 641
Fsp4HI GCNGC 2 cut(s) 324, 443
FspBI CTAG 1 cut(s) 161
GluI GCNGC 2 cut(s) 324, 443
GsaI CCCAGC 1 cut(s) 160
GsuI CTGGAG 2 cut(s) 616, 652
HaeIII GGCC 2 cut(s) 605, 617
HapII CCGG 1 cut(s) 53
HgaI GACGC 1 cut(s) 493
Hin1II CATG 6 cut(s) 121, 142, 440, 502, 577, 604
HinfI GANTC 6 cut(s) 28, 262, 278, 295, 632, 665
HpaII CCGG 1 cut(s) 53
Hpy188I TCNGA 1 cut(s) 27
Hpy188III TCNNGA 6 cut(s) 59, 65, 118, 251, 469, 595
HpyAV CCTTC 2 cut(s) 115, 616
HpyCH4III ACNGT 1 cut(s) 553
HpyCH4V TGCA 4 cut(s) 389, 411, 429, 442
HpyF10VI GCNNNNNNNGC 3 cut(s) 144, 219, 332
HpyF3I CTNAG 2 cut(s) 231, 640
Hsp92II CATG 6 cut(s) 121, 142, 440, 502, 577, 604
Kzo9I GATC 3 cut(s) 61, 142, 330
LguI GCTCTTC 1 cut(s) 9
LmnI GCTCC 2 cut(s) 124, 597
Lsp1109I GCAGC 2 cut(s) 310, 454
LweI GCATC 2 cut(s) 222, 438
MabI ACCWGGT 1 cut(s) 367
MaeI CTAG 1 cut(s) 161
MaeIII GTNAC 2 cut(s) 173, 534
MalI GATC 3 cut(s) 63, 144, 332
MboI GATC 3 cut(s) 61, 142, 330
MboII GAAGA 4 cut(s) 26, 80, 217, 583
MfeI CAATTG 1 cut(s) 475
MhlI GDGCHC 3 cut(s) 425, 573, 641
MluCI AATT 3 cut(s) 93, 461, 475
MlyI GAGTC 2 cut(s) 304, 659
MnlI CCTC 8 cut(s) 64, 80, 157, 188, 265, 268, 590, 660
MslI CAYNNNNRTG 2 cut(s) 317, 353
MspA1I CMGCKG 1 cut(s) 644
MspI CCGG 1 cut(s) 53
MspR9I CCNGG 3 cut(s) 369, 458, 670
MunI CAATTG 1 cut(s) 475
Mva1269I GAATGC 1 cut(s) 391
MvaI CCWGG 3 cut(s) 369, 458, 670
MwoI GCNNNNNNNGC 3 cut(s) 144, 219, 332
NdeII GATC 3 cut(s) 61, 142, 330
NlaIII CATG 6 cut(s) 121, 142, 440, 502, 577, 604
NmuCI GTSAC 2 cut(s) 173, 534
PagI TCATGA 1 cut(s) 117
PciSI GCTCTTC 1 cut(s) 9
PctI GAATGC 1 cut(s) 391
PfeI GAWTC 4 cut(s) 28, 262, 278, 632
PfoI TCCNGGA 1 cut(s) 456
PkrI GCNGC 2 cut(s) 325, 444
Ple19I CGATCG 1 cut(s) 333
PleI GAGTC 2 cut(s) 303, 659
PpsI GAGTC 2 cut(s) 303, 659
Psp124BI GAGCTC 1 cut(s) 641
Psp6I CCWGG 3 cut(s) 367, 456, 668
PspFI CCCAGC 1 cut(s) 156
PspGI CCWGG 3 cut(s) 367, 456, 668
PvuI CGATCG 1 cut(s) 333
PvuII CAGCTG 1 cut(s) 644
RsaI GTAC 1 cut(s) 648
RsaNI GTAC 1 cut(s) 647
RseI CAYNNNNRTG 2 cut(s) 317, 353
SacI GAGCTC 1 cut(s) 641
SapI GCTCTTC 1 cut(s) 9
SatI GCNGC 2 cut(s) 324, 443
Sau3AI GATC 3 cut(s) 61, 142, 330
SchI GAGTC 2 cut(s) 304, 659
ScrFI CCNGG 3 cut(s) 369, 458, 670
SduI GDGCHC 3 cut(s) 425, 573, 641
SexAI ACCWGGT 1 cut(s) 367
SfaNI GCATC 2 cut(s) 222, 438
SmiMI CAYNNNNRTG 2 cut(s) 317, 353
SmlI CTYRAG 2 cut(s) 179, 249
SmoI CTYRAG 2 cut(s) 179, 249
Sse9I AATT 3 cut(s) 93, 461, 475
SspMI CTAG 1 cut(s) 161
SstI GAGCTC 1 cut(s) 641
StyD4I CCNGG 3 cut(s) 367, 456, 668
TaaI ACNGT 1 cut(s) 553
TaqI TCGA 3 cut(s) 37, 64, 635
TaqII GACCGA 1 cut(s) 522
TasI AATT 3 cut(s) 93, 461, 475
TfiI GAWTC 4 cut(s) 28, 262, 278, 632
TscAI CASTG 2 cut(s) 244, 273
TseFI GTSAC 2 cut(s) 173, 534
TseI GCWGC 2 cut(s) 323, 442
Tsp45I GTSAC 2 cut(s) 173, 534
TspDTI ATGAA 3 cut(s) 134, 218, 454
TspRI CASTG 2 cut(s) 244, 273
XapI RAATTY 1 cut(s) 461
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.