pycom12g20080

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0000404
Physical Location & Seq
Forward (+)
21725592 .. 21726779
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g20080.2

Sequence Viewer

Length: 957 bp
ATGGAGATCAGACCGCACGATGAAGATGAAGTGTACAAGGCATCACGGACCGGGAGTGTAGAATCCTTAAACAGGTTGATCAAAAAAGACCCAGACATTCTATGGAGAATATCCCTGCAGACCGGCAAAACCGGAACCCCCTTACATGTGTCGGCTTTGCACGGCCACACTGAGTTTACCAAAGCCCTTTGCACTAACAATCCCAAACTTGCAGAGGGGGTGGACGCCGATAGACGCACGCCCCTGCACTTGGCTTCTGCTAAGGGCCACAAGGAGACCGTCGAAGCTTTGTTATCTGTGTATGCTGGTGCGTGCTCGGATTTTGATGAAAATGGAAGAATCCCTCTTCACTACGCAGCCATGAGAGGAGAAGTTGAGGTGCTCCAGAAGTTAATTGATGAAAATCCTCGGTCCATTTATGCCAAAGTTGAAAACAGATCGATAGAAACAGTTTTGCACTTGTGTATTATACACAACCAGTTAGAGTGCTTGAAACTGTTGGTTGGAAGAGACGACAGAAATGGTGAGTTCCTCAACTCAAGAGCTGGCTGTGACGGTGGTGTGACCATCCTGCACTTAGCTTTGATGCTAAGGCAAATTAAGATCTGCAACCCACTCCCTTCCCTTACCTCCCGTTCAACTGGAACAAAAGAAAGAATCCCGCCCATCTGCATCTCAAGAAGTTGTAGAAGTATTAGCAGCAGCACCGCTGGCGGTCATCGGTTCGGCGTCTTCCGCCGTCACCAAGTCCGAATCCGAAGACAACTCCAGTTGGCTGGCCAGCCGACGGAAAGTTCAGTCTCGAATGGGTTAGGAACCTCGTGTTTGTTTTTTTATTGGGCATCTAGAAATCTGGACCCGACCCAATTGCCCGTGTTTCCCGTAGTGGTTGTCGATAGCTTGGTCGTCTGCCCATCTAAGATCCTCCACAAAGAGGCCAAACGTCGATCAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

319

Amino Acids

35.31

Weight (kDa)

9.2

Isoelectric Point (pI)

54.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13950 AT4G13266
fragaria_vesca FvH4_7g06240 FvH4_7g06250 FvH4_7g06270
malus_domestica MD12G1213300.v1.1 MD12G1213900.v1.1 MD12G1214100.v1.1 MD12G1214700.v1.1 MD12G1214800.v1.1
prunus_persica Prupe.2G107700_v2.0.a1 Prupe.3G142200_v2.0.a1 Prupe.3G154100_v2.0.a1 Prupe.3G154300_v2.0.a1 Prupe.3G154900_v2.0.a1 Prupe.3G155100_v2.0.a1
pyrus_communis pycom12g20010 pycom12g20080 pycom12g20090 pycom12g20100
rosa_chinensis RchiOBHm_Chr1g0341001 RchiOBHm_Chr1g0341021 RchiOBHm_Chr1g0341891 RchiOBHm_Chr1g0342341 RchiOBHm_Chr3g0490431
rosa_laevigata RLG00000022889 RLG00000029016 RLG00000029039 RLG00000029059 RLG00000029061 RLG00000029065 RLG00000029115 RLG00000029155 RLG00000033311 RLG00000033312
rosa_multiflora Rmu_sc0000404.1_g000006 Rmu_sc0001836.1_g000015 Rmu_sc0001836.1_g000050 Rmu_sc0002200.1_g000003 Rmu_sc0003862.1_g000020 Rmu_sc0005193.1_g000001 Rmu_sc0005193.1_g000002 Rmu_sc0008453.1_g000008 Rmu_sc0008453.1_g000009 Rmu_sc0008453.1_g000010 Rmu_sc0008862.1_g000001 Rmu_sc0011195.1_g000003 Rmu_sc0014077.1_g000001
rosa_roxburghii Rroxscaffold_4G00311670 Rroxscaffold_4G00311730 Rroxscaffold_4G00311750 Rroxscaffold_4G00312290 Rroxscaffold_6G00393430
rosa_rugosa Rorug01G0153800.1 Rorug01G0153900.1 Rorug01G0160100.1 Rorug03G0243800
rosa_samantha Rh1AG166400 Rh1AG168200 Rh1AG174100 Rh1AG174700 Rh1AG179000 Rh1BG132600 Rh1BG136500 Rh1BG142200 Rh1BG142800 Rh1BG143200 Rh1BG146200 Rh1BG147000 Rh1BG147100 Rh1CG154800 Rh1CG161900 Rh1CG162700 Rh1CG166000 Rh1DG167500 Rh1DG169900 Rh1DG174000 Rh1DG174400 Rh1DG174700 Rh1DG174800 Rh1DG178200 Rh1DG179000 Rh1DG179100 Rh3AG292800 Rh3BG329600 Rh3CG326900 Rh3DG326200 Rh5BG218300 Rh7CG326800 Rh7DG307100
rosa_wichuraiana Rw0G006190 Rw0G007830 Rw0G009970 Rw0G009980 Rw1G013880 Rw1G014000 Rw1G014500 Rw1G014560 Rw1G014920 Rw3G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 14, 662, 708, 714, 736
AclWI GGATC 1 cut(s) 916
AcoI YGGCCR 2 cut(s) 163, 778
AcyI GRCGYC 2 cut(s) 225, 729
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 4 cut(s) 72, 250, 787, 934
AflIII ACRYGT 1 cut(s) 145
AgsI TTSAA 3 cut(s) 431, 493, 639
AluBI AGCT 4 cut(s) 287, 545, 581, 900
AluI AGCT 4 cut(s) 287, 545, 581, 900
Alw21I GWGCWC 2 cut(s) 317, 384
Alw26I GTCTC 3 cut(s) 269, 504, 805
AlwI GGATC 1 cut(s) 916
AoxI GGCC 4 cut(s) 163, 265, 778, 936
ApeKI GCWGC 3 cut(s) 356, 699, 702
AspS9I GGNCC 4 cut(s) 48, 265, 411, 856
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 2 cut(s) 536, 734
AvaII GGWCC 3 cut(s) 48, 411, 856
BalI TGGCCA 1 cut(s) 780
BauI CACGAG 1 cut(s) 820
BbsI GAAGAC 2 cut(s) 724, 766
Bbv12I GWGCWC 2 cut(s) 317, 384
BbvI GCAGC 3 cut(s) 368, 711, 714
BccI CCATC 3 cut(s) 575, 674, 922
BceAI ACGGC 2 cut(s) 178, 723
BcgI CGANNNNNNTGC 2 cut(s) 850, 884
BclI TGATCA 1 cut(s) 78
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 269, 504, 805
BfaI CTAG 1 cut(s) 846
BfmI CTRYAG 1 cut(s) 116
BglII AGATCT 1 cut(s) 603
BisI GCNGC 3 cut(s) 357, 700, 703
BlsI GCNGC 3 cut(s) 358, 701, 704
Bme1390I CCNGG 1 cut(s) 52
Bme18I GGWCC 3 cut(s) 48, 411, 856
BmgT120I GGNCC 4 cut(s) 48, 265, 411, 856
BmiI GGNNCC 3 cut(s) 136, 817, 858
BmrFI CCNGG 1 cut(s) 52
BmsI GCATC 4 cut(s) 50, 576, 681, 851
BpiI GAAGAC 2 cut(s) 724, 766
BpmI CTGGAG 2 cut(s) 368, 752
Bpu10I CCTNAGC 2 cut(s) 261, 590
BpuEI CTTGAG 2 cut(s) 523, 661
BpuMI CCSGG 1 cut(s) 52
Bsa29I ATCGAT 1 cut(s) 440
BsaBI GATNNNNATC 1 cut(s) 402
BsaHI GRCGYC 2 cut(s) 225, 729
BsaI GGTCTC 1 cut(s) 269
BsaJI CCNNGG 1 cut(s) 407
BsaWI WCCGGW 1 cut(s) 131
Bsc4I CCNNNNNNNGG 4 cut(s) 72, 250, 787, 934
Bse118I RCCGGY 1 cut(s) 122
Bse1I ACTGG 3 cut(s) 478, 646, 769
Bse8I GATNNNNATC 1 cut(s) 402
BseCI ATCGAT 1 cut(s) 440
BseDI CCNNGG 1 cut(s) 407
BseGI GGATG 1 cut(s) 567
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 4 cut(s) 72, 250, 787, 934
BseMII CTCAG 1 cut(s) 162
BseNI ACTGG 3 cut(s) 478, 646, 769
BseRI GAGGAG 1 cut(s) 381
BseXI GCAGC 3 cut(s) 368, 711, 714
BsgI GTGCAG 2 cut(s) 230, 557
BshFI GGCC 4 cut(s) 165, 267, 780, 938
BshVI ATCGAT 1 cut(s) 440
BsiHKAI GWGCWC 2 cut(s) 317, 384
BsiSI CCGG 3 cut(s) 51, 123, 132
BslI CCNNNNNNNGG 4 cut(s) 72, 250, 787, 934
BsmAI GTCTC 3 cut(s) 269, 504, 805
BsmBI CGTCTC 1 cut(s) 504
BsnI GGCC 4 cut(s) 165, 267, 780, 938
Bso31I GGTCTC 1 cut(s) 269
Bsp1286I GDGCHC 2 cut(s) 317, 384
Bsp1407I TGTACA 1 cut(s) 33
Bsp143I GATC 6 cut(s) 6, 78, 437, 603, 921, 947
BspACI CCGC 5 cut(s) 14, 662, 708, 714, 736
BspANI GGCC 4 cut(s) 165, 267, 780, 938
BspCNI CTCAG 1 cut(s) 163
BspDI ATCGAT 1 cut(s) 440
BspLI GGNNCC 3 cut(s) 136, 817, 858
BspMAI CTGCAG 1 cut(s) 120
BspPI GGATC 1 cut(s) 916
BspTNI GGTCTC 1 cut(s) 269
BsrFI RCCGGY 1 cut(s) 122
BsrGI TGTACA 1 cut(s) 33
BsrI ACTGG 3 cut(s) 478, 646, 769
BssAI RCCGGY 1 cut(s) 122
BssECI CCNNGG 1 cut(s) 407
BssMI GATC 6 cut(s) 6, 78, 437, 603, 921, 947
BssNI GRCGYC 2 cut(s) 225, 729
BssSI CACGAG 1 cut(s) 820
Bst2BI CACGAG 1 cut(s) 820
Bst4CI ACNGT 4 cut(s) 280, 451, 498, 557
Bst6I CTCTTC 2 cut(s) 351, 502
BstACI GRCGYC 2 cut(s) 225, 729
BstAUI TGTACA 1 cut(s) 33
BstC8I GCNNGC 6 cut(s) 239, 313, 547, 712, 778, 782
BstDEI CTNAG 5 cut(s) 171, 261, 577, 590, 918
BstENI CCTNNNNNAGG 1 cut(s) 70
BstF5I GGATG 1 cut(s) 567
BstKTI GATC 6 cut(s) 9, 81, 440, 606, 924, 950
BstMAI GTCTC 3 cut(s) 269, 504, 805
BstMBI GATC 6 cut(s) 6, 78, 437, 603, 921, 947
BstMWI GCNNNNNNNGC 2 cut(s) 711, 735
BstNSI RCATGY 1 cut(s) 149
BstSCI CCNGG 1 cut(s) 50
BstSFI CTRYAG 1 cut(s) 116
BstV1I GCAGC 3 cut(s) 368, 711, 714
BstV2I GAAGAC 2 cut(s) 724, 766
BstX2I RGATCY 2 cut(s) 603, 921
BstXI CCANNNNNNTGG 1 cut(s) 776
BstYI RGATCY 2 cut(s) 603, 921
Bsu15I ATCGAT 1 cut(s) 440
BsuRI GGCC 4 cut(s) 165, 267, 780, 938
BsuTUI ATCGAT 1 cut(s) 440
BtsCI GGATG 1 cut(s) 567
BtsIMutI CAGTG 1 cut(s) 168
Cac8I GCNNGC 6 cut(s) 239, 313, 547, 712, 778, 782
Cfr10I RCCGGY 1 cut(s) 122
Cfr13I GGNCC 4 cut(s) 48, 265, 411, 856
ClaI ATCGAT 1 cut(s) 440
CpoI CGGWCCG 1 cut(s) 48
CseI GACGC 3 cut(s) 233, 243, 718
Csp6I GTAC 1 cut(s) 34
CspI CGGWCCG 1 cut(s) 48
CviAII CATG 2 cut(s) 146, 361
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 5 cut(s) 171, 261, 577, 590, 918
DpnI GATC 6 cut(s) 8, 80, 439, 605, 923, 949
DpnII GATC 6 cut(s) 6, 78, 437, 603, 921, 947
EaeI YGGCCR 2 cut(s) 163, 778
Eam1104I CTCTTC 2 cut(s) 351, 502
EarI CTCTTC 2 cut(s) 351, 502
EciI GGCGGA 1 cut(s) 725
Eco31I GGTCTC 1 cut(s) 269
Eco47I GGWCC 3 cut(s) 48, 411, 856
EcoNI CCTNNNNNAGG 1 cut(s) 70
Esp3I CGTCTC 1 cut(s) 504
FaeI CATG 2 cut(s) 149, 364
FaiI YATR 6 cut(s) 103, 147, 303, 362, 420, 470
FatI CATG 2 cut(s) 145, 360
FauI CCCGC 1 cut(s) 669
FbaI TGATCA 1 cut(s) 78
Fnu4HI GCNGC 3 cut(s) 357, 700, 703
FokI GGATG 1 cut(s) 554
Fsp4HI GCNGC 3 cut(s) 357, 700, 703
FspBI CTAG 1 cut(s) 846
GluI GCNGC 3 cut(s) 357, 700, 703
GsuI CTGGAG 2 cut(s) 368, 752
HaeIII GGCC 4 cut(s) 165, 267, 780, 938
HapII CCGG 3 cut(s) 51, 123, 132
HgaI GACGC 3 cut(s) 233, 243, 718
Hin1I GRCGYC 2 cut(s) 225, 729
Hin1II CATG 2 cut(s) 149, 364
HindIII AAGCTT 1 cut(s) 285
HinfI GANTC 4 cut(s) 62, 339, 657, 753
HpaII CCGG 3 cut(s) 51, 123, 132
HphI GGTGA 2 cut(s) 536, 734
Hpy166II GTNNAC 3 cut(s) 34, 177, 223
Hpy188I TCNGA 4 cut(s) 11, 319, 752, 758
Hpy188III TCNNGA 6 cut(s) 385, 540, 678, 802, 846, 854
Hpy8I GTNNAC 3 cut(s) 34, 177, 223
Hpy99I CGWCG 3 cut(s) 284, 790, 948
HpyAV CCTTC 1 cut(s) 630
HpyCH4III ACNGT 4 cut(s) 280, 451, 498, 557
HpyCH4IV ACGT 1 cut(s) 943
HpyCH4V TGCA 9 cut(s) 118, 160, 192, 212, 247, 457, 574, 609, 672
HpyF10VI GCNNNNNNNGC 2 cut(s) 711, 735
HpyF3I CTNAG 5 cut(s) 171, 261, 577, 590, 918
HpySE526I ACGT 1 cut(s) 943
Hsp92I GRCGYC 2 cut(s) 225, 729
Hsp92II CATG 2 cut(s) 149, 364
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 6 cut(s) 6, 78, 437, 603, 921, 947
LmnI GCTCC 1 cut(s) 387
Lsp1109I GCAGC 3 cut(s) 368, 711, 714
LweI GCATC 4 cut(s) 50, 576, 681, 851
MaeI CTAG 1 cut(s) 846
MaeII ACGT 1 cut(s) 943
MaeIII GTNAC 3 cut(s) 551, 562, 740
MalI GATC 6 cut(s) 8, 80, 439, 605, 923, 949
MboI GATC 6 cut(s) 6, 78, 437, 603, 921, 947
MboII GAAGA 6 cut(s) 35, 338, 348, 519, 724, 771
MfeI CAATTG 1 cut(s) 866
MflI RGATCY 2 cut(s) 603, 921
MhlI GDGCHC 2 cut(s) 317, 384
MlsI TGGCCA 1 cut(s) 780
MluCI AATT 3 cut(s) 393, 597, 866
MluNI TGGCCA 1 cut(s) 780
MmeI TCCRAC 1 cut(s) 484
Mox20I TGGCCA 1 cut(s) 780
MscI TGGCCA 1 cut(s) 780
MseI TTAA 3 cut(s) 68, 392, 600
Msp20I TGGCCA 1 cut(s) 780
MspA1I CMGCKG 1 cut(s) 710
MspI CCGG 3 cut(s) 51, 123, 132
MspR9I CCNGG 1 cut(s) 52
MunI CAATTG 1 cut(s) 866
MwoI GCNNNNNNNGC 2 cut(s) 711, 735
NciI CCSGG 1 cut(s) 52
NdeII GATC 6 cut(s) 6, 78, 437, 603, 921, 947
NlaIII CATG 2 cut(s) 149, 364
NlaIV GGNNCC 3 cut(s) 136, 817, 858
NmuCI GTSAC 3 cut(s) 551, 562, 740
NspI RCATGY 1 cut(s) 149
PciI ACATGT 1 cut(s) 145
PfeI GAWTC 4 cut(s) 62, 339, 657, 753
PkrI GCNGC 3 cut(s) 358, 701, 704
PscI ACATGT 1 cut(s) 145
PspN4I GGNNCC 3 cut(s) 136, 817, 858
PspPI GGNCC 4 cut(s) 48, 265, 411, 856
PstI CTGCAG 1 cut(s) 120
PsuI RGATCY 2 cut(s) 603, 921
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
Rsr2I CGGWCCG 1 cut(s) 48
RsrII CGGWCCG 1 cut(s) 48
SaqAI TTAA 3 cut(s) 68, 392, 600
SatI GCNGC 3 cut(s) 357, 700, 703
Sau3AI GATC 6 cut(s) 6, 78, 437, 603, 921, 947
Sau96I GGNCC 4 cut(s) 48, 265, 411, 856
ScrFI CCNGG 1 cut(s) 52
SduI GDGCHC 2 cut(s) 317, 384
SetI ASST 9 cut(s) 77, 289, 381, 547, 583, 632, 821, 902, 946
SfaNI GCATC 4 cut(s) 50, 576, 681, 851
SfcI CTRYAG 1 cut(s) 116
SinI GGWCC 3 cut(s) 48, 411, 856
SmlI CTYRAG 2 cut(s) 538, 676
SmoI CTYRAG 2 cut(s) 538, 676
Sse9I AATT 3 cut(s) 393, 597, 866
SsiI CCGC 5 cut(s) 14, 662, 708, 714, 736
SspMI CTAG 1 cut(s) 846
StyD4I CCNGG 1 cut(s) 50
TaaI ACNGT 4 cut(s) 280, 451, 498, 557
TaiI ACGT 1 cut(s) 946
TaqI TCGA 5 cut(s) 282, 440, 803, 894, 946
TaqII GACCGA 1 cut(s) 399
TasI AATT 3 cut(s) 393, 597, 866
TatI WGTACW 1 cut(s) 33
TfiI GAWTC 4 cut(s) 62, 339, 657, 753
Tru1I TTAA 3 cut(s) 68, 392, 600
Tru9I TTAA 3 cut(s) 68, 392, 600
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 3 cut(s) 551, 562, 740
TseI GCWGC 3 cut(s) 356, 699, 702
Tsp45I GTSAC 3 cut(s) 551, 562, 740
TspDTI ATGAA 4 cut(s) 36, 42, 342, 414
TspGWI ACGGA 2 cut(s) 61, 803
TspRI CASTG 1 cut(s) 175
VpaK11BI GGWCC 3 cut(s) 48, 411, 856
XagI CCTNNNNNAGG 1 cut(s) 70
XbaI TCTAGA 1 cut(s) 845
XceI RCATGY 1 cut(s) 149
XcmI CCANNNNNNNNNTGG 1 cut(s) 99
XspI CTAG 1 cut(s) 846
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.