Rw0G009970

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00413
OG0000404
Physical Location & Seq
Forward (+)
4825 .. 5752
928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G009970.1

Sequence Viewer

Length: 627 bp
ATGGAGATTAGAGAAGATCACCATGAAGACGATACAACAAGAGCACTCTATGAGGCAGCAGATGCCGGCTCAGTACCATCTTTAGAAAGATTGATCCAGAAAGATCCTCGCATCCTCAACAAAGTTTCACTCACTCACTTCAGTGAAACTCCCCTCCACATCTCAGCTCTGCTCGGCCACATTGATTTCACCAAGGCTATTCTTACTCATAAGTACTCGAGGCTTGCCACCGTGAGGGACTCCTTGAGACGCCTGCCCCTCCACTTGGCATCTGCCGAGGGCCATAAGGAGACTGTTCAAGCTCTTCTGAAAGCCTACCCCGACGCTTGCTTGTTTCGGGATCAGGATGGAAGAATCCCTCTTCACTATGCCGCTATGAGAGGACACGCTGAGGTCTTAGCTGAGCTGATGCGCGCAAACCCCGAATCAGTCACGTTAAGCGTTCTTGACAGAGGAGACACGGTGTTCCACTTATGTGTTACTTATAACCAATTAGAATGCTTGAAGCTATTGGTGGAAGAGGTAGGAGACAATAGAGACCTCCTCAATGCTAGAGCTGGATGCGATGGTGGTATGACCATCCTCGAATTAGCTTTTAGAATTTCATTGAAGCTGAATCTAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 PGG

Protein Analysis

208

Amino Acids

23.33

Weight (kDa)

5.87

Isoelectric Point (pI)

37.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 16 - 106 2.3e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 87 - 133 2.5e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 112 - 174 2.3e-09 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000404)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13950 AT4G13266
fragaria_vesca FvH4_7g06240 FvH4_7g06250 FvH4_7g06270
malus_domestica MD12G1213300.v1.1 MD12G1213900.v1.1 MD12G1214100.v1.1 MD12G1214700.v1.1 MD12G1214800.v1.1
prunus_persica Prupe.2G107700_v2.0.a1 Prupe.3G142200_v2.0.a1 Prupe.3G154100_v2.0.a1 Prupe.3G154300_v2.0.a1 Prupe.3G154900_v2.0.a1 Prupe.3G155100_v2.0.a1
pyrus_communis pycom12g20010 pycom12g20080 pycom12g20090 pycom12g20100
rosa_chinensis RchiOBHm_Chr1g0341001 RchiOBHm_Chr1g0341021 RchiOBHm_Chr1g0341891 RchiOBHm_Chr1g0342341 RchiOBHm_Chr3g0490431
rosa_laevigata RLG00000022889 RLG00000029016 RLG00000029039 RLG00000029059 RLG00000029061 RLG00000029065 RLG00000029115 RLG00000029155 RLG00000033311 RLG00000033312
rosa_multiflora Rmu_sc0000404.1_g000006 Rmu_sc0001836.1_g000015 Rmu_sc0001836.1_g000050 Rmu_sc0002200.1_g000003 Rmu_sc0003862.1_g000020 Rmu_sc0005193.1_g000001 Rmu_sc0005193.1_g000002 Rmu_sc0008453.1_g000008 Rmu_sc0008453.1_g000009 Rmu_sc0008453.1_g000010 Rmu_sc0008862.1_g000001 Rmu_sc0011195.1_g000003 Rmu_sc0014077.1_g000001
rosa_roxburghii Rroxscaffold_4G00311670 Rroxscaffold_4G00311730 Rroxscaffold_4G00311750 Rroxscaffold_4G00312290 Rroxscaffold_6G00393430
rosa_rugosa Rorug01G0153800.1 Rorug01G0153900.1 Rorug01G0160100.1 Rorug03G0243800
rosa_samantha Rh1AG166400 Rh1AG168200 Rh1AG174100 Rh1AG174700 Rh1AG179000 Rh1BG132600 Rh1BG136500 Rh1BG142200 Rh1BG142800 Rh1BG143200 Rh1BG146200 Rh1BG147000 Rh1BG147100 Rh1CG154800 Rh1CG161900 Rh1CG162700 Rh1CG166000 Rh1DG167500 Rh1DG169900 Rh1DG174000 Rh1DG174400 Rh1DG174700 Rh1DG174800 Rh1DG178200 Rh1DG179000 Rh1DG179100 Rh3AG292800 Rh3BG329600 Rh3CG326900 Rh3DG326200 Rh5BG218300 Rh7CG326800 Rh7DG307100
rosa_wichuraiana Rw0G006190 Rw0G007830 Rw0G009970 Rw0G009980 Rw1G013880 Rw1G014000 Rw1G014500 Rw1G014560 Rw1G014920 Rw3G025950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 486
AccII CGCG 1 cut(s) 414
AciI CCGC 1 cut(s) 372
AclWI GGATC 3 cut(s) 88, 98, 348
AcoI YGGCCR 1 cut(s) 175
AcsI RAATTY 1 cut(s) 600
AcuI CTGAAG 1 cut(s) 124
AcyI GRCGYC 1 cut(s) 250
AfaI GTAC 2 cut(s) 75, 215
AfiI CCNNNNNNNGG 2 cut(s) 234, 265
AgsI TTSAA 3 cut(s) 299, 505, 610
AleI CACNNNNGTG 2 cut(s) 141, 474
AluBI AGCT 8 cut(s) 167, 302, 401, 406, 508, 557, 593, 613
AluI AGCT 8 cut(s) 167, 302, 401, 406, 508, 557, 593, 613
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 5 cut(s) 241, 284, 450, 522, 531
AlwI GGATC 3 cut(s) 88, 98, 348
Ama87I CYCGRG 1 cut(s) 217
AoxI GGCC 2 cut(s) 175, 280
ApeKI GCWGC 1 cut(s) 56
ApoI RAATTY 1 cut(s) 600
AspLEI GCGC 2 cut(s) 414, 416
AspS9I GGNCC 1 cut(s) 280
AsuHPI GGTGA 2 cut(s) 11, 181
AvaI CYCGRG 1 cut(s) 217
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 1 cut(s) 46
BbvCI CCTCAGC 1 cut(s) 390
BbvI GCAGC 1 cut(s) 68
BccI CCATC 4 cut(s) 85, 341, 560, 587
BcoDI GTCTC 5 cut(s) 241, 284, 450, 522, 531
BfaI CTAG 2 cut(s) 552, 620
BisI GCNGC 2 cut(s) 57, 372
BlpI GCTNAGC 1 cut(s) 402
BlsI GCNGC 2 cut(s) 58, 373
BmcAI AGTACT 1 cut(s) 215
BmeT110I CYCGRG 1 cut(s) 217
BmgT120I GGNCC 1 cut(s) 280
BmsI GCATC 5 cut(s) 52, 120, 278, 399, 551
BpiI GAAGAC 1 cut(s) 33
BplI GAGNNNNNCTC 2 cut(s) 528, 560
Bpu10I CCTNAGC 1 cut(s) 390
Bpu1102I GCTNAGC 1 cut(s) 402
BpuEI CTTGAG 1 cut(s) 265
BsaHI GRCGYC 1 cut(s) 250
BsaI GGTCTC 1 cut(s) 531
BsaJI CCNNGG 2 cut(s) 192, 276
BsaXI ACNNNNNCTCC 2 cut(s) 447, 477
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 265
Bse118I RCCGGY 1 cut(s) 65
BseDI CCNNGG 2 cut(s) 192, 276
BseGI GGATG 4 cut(s) 111, 352, 566, 579
BseLI CCNNNNNNNGG 2 cut(s) 234, 265
BseMII CTCAG 4 cut(s) 84, 177, 381, 393
BsePI GCGCGC 1 cut(s) 412
BseRI GAGGAG 2 cut(s) 468, 533
BseXI GCAGC 1 cut(s) 68
Bsh1236I CGCG 1 cut(s) 414
BshFI GGCC 2 cut(s) 177, 282
BsiHKAI GWGCWC 1 cut(s) 46
BsiHKCI CYCGRG 1 cut(s) 217
BsiSI CCGG 1 cut(s) 66
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 2 cut(s) 234, 265
BsmAI GTCTC 5 cut(s) 241, 284, 450, 522, 531
BsmBI CGTCTC 1 cut(s) 241
BsmFI GGGAC 1 cut(s) 251
BsmI GAATGC 1 cut(s) 503
BsnI GGCC 2 cut(s) 177, 282
Bso31I GGTCTC 1 cut(s) 531
BsoBI CYCGRG 1 cut(s) 217
Bsp1286I GDGCHC 1 cut(s) 46
Bsp143I GATC 4 cut(s) 16, 93, 103, 340
Bsp1720I GCTNAGC 1 cut(s) 402
BspACI CCGC 1 cut(s) 372
BspANI GGCC 2 cut(s) 177, 282
BspCNI CTCAG 4 cut(s) 83, 176, 382, 394
BspFNI CGCG 1 cut(s) 414
BspPI GGATC 3 cut(s) 88, 98, 348
BspQI GCTCTTC 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 531
BsrFI RCCGGY 1 cut(s) 65
BssAI RCCGGY 1 cut(s) 65
BssECI CCNNGG 2 cut(s) 192, 276
BssHII GCGCGC 1 cut(s) 412
BssMI GATC 4 cut(s) 16, 93, 103, 340
BssNI GRCGYC 1 cut(s) 250
BssT1I CCWWGG 1 cut(s) 192
Bst4CI ACNGT 3 cut(s) 232, 295, 463
Bst6I CTCTTC 3 cut(s) 309, 366, 513
BstACI GRCGYC 1 cut(s) 250
BstAPI GCANNNNNTGC 1 cut(s) 62
BstC8I GCNNGC 5 cut(s) 67, 225, 254, 328, 414
BstDEI CTNAG 5 cut(s) 70, 163, 390, 397, 402
BstF5I GGATG 4 cut(s) 111, 352, 566, 579
BstFNI CGCG 1 cut(s) 414
BstHHI GCGC 2 cut(s) 414, 416
BstKTI GATC 4 cut(s) 19, 96, 106, 343
BstMAI GTCTC 5 cut(s) 241, 284, 450, 522, 531
BstMBI GATC 4 cut(s) 16, 93, 103, 340
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstUI CGCG 1 cut(s) 414
BstV1I GCAGC 1 cut(s) 68
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 103
BstYI RGATCY 1 cut(s) 103
BsuRI GGCC 2 cut(s) 177, 282
BtgZI GCGATG 1 cut(s) 579
BtsCI GGATG 4 cut(s) 111, 352, 566, 579
BtsIMutI CAGTG 1 cut(s) 148
Cac8I GCNNGC 5 cut(s) 67, 225, 254, 328, 414
CfoI GCGC 2 cut(s) 414, 416
Cfr10I RCCGGY 1 cut(s) 65
Cfr13I GGNCC 1 cut(s) 280
CseI GACGC 2 cut(s) 258, 332
Csp6I GTAC 2 cut(s) 74, 214
CviAII CATG 1 cut(s) 23
CviQI GTAC 2 cut(s) 74, 214
DdeI CTNAG 5 cut(s) 70, 163, 390, 397, 402
DpnI GATC 4 cut(s) 18, 95, 105, 342
DpnII GATC 4 cut(s) 16, 93, 103, 340
EaeI YGGCCR 1 cut(s) 175
Eam1104I CTCTTC 3 cut(s) 309, 366, 513
EarI CTCTTC 3 cut(s) 309, 366, 513
Eco130I CCWWGG 1 cut(s) 192
Eco31I GGTCTC 1 cut(s) 531
Eco57I CTGAAG 1 cut(s) 124
Eco88I CYCGRG 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 192
ErhI CCWWGG 1 cut(s) 192
Esp3I CGTCTC 1 cut(s) 241
FaeI CATG 1 cut(s) 26
FaiI YATR 9 cut(s) 24, 51, 210, 285, 369, 377, 475, 486, 575
FaqI GGGAC 1 cut(s) 251
FatI CATG 1 cut(s) 22
Fnu4HI GCNGC 2 cut(s) 57, 372
FokI GGATG 4 cut(s) 98, 359, 566, 573
Fsp4HI GCNGC 2 cut(s) 57, 372
FspBI CTAG 2 cut(s) 552, 620
GlaI GCGC 2 cut(s) 413, 415
GluI GCNGC 2 cut(s) 57, 372
HaeIII GGCC 2 cut(s) 177, 282
HapII CCGG 1 cut(s) 66
HgaI GACGC 2 cut(s) 258, 332
HhaI GCGC 2 cut(s) 414, 416
Hin1I GRCGYC 1 cut(s) 250
Hin1II CATG 1 cut(s) 26
Hin6I GCGC 2 cut(s) 412, 414
HinP1I GCGC 2 cut(s) 412, 414
HinfI GANTC 4 cut(s) 239, 354, 425, 616
HpaII CCGG 1 cut(s) 66
HphI GGTGA 2 cut(s) 11, 181
Hpy188I TCNGA 1 cut(s) 309
Hpy188III TCNNGA 5 cut(s) 97, 338, 344, 446, 620
Hpy99I CGWCG 1 cut(s) 326
HpyCH4III ACNGT 3 cut(s) 232, 295, 463
HpyCH4IV ACGT 1 cut(s) 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 5 cut(s) 70, 163, 390, 397, 402
HpySE526I ACGT 1 cut(s) 434
Hsp92I GRCGYC 1 cut(s) 250
Hsp92II CATG 1 cut(s) 26
HspAI GCGC 2 cut(s) 412, 414
KroI GCCGGC 1 cut(s) 65
KroNI GCCGGC 1 cut(s) 67
Kzo9I GATC 4 cut(s) 16, 93, 103, 340
LguI GCTCTTC 1 cut(s) 309
LpnPI CCDG 5 cut(s) 79, 110, 266, 329, 543
Lsp1109I GCAGC 1 cut(s) 68
LweI GCATC 5 cut(s) 52, 120, 278, 399, 551
MaeI CTAG 2 cut(s) 552, 620
MaeII ACGT 1 cut(s) 434
MaeIII GTNAC 2 cut(s) 430, 478
MalI GATC 4 cut(s) 18, 95, 105, 342
MboI GATC 4 cut(s) 16, 93, 103, 340
MboII GAAGA 6 cut(s) 26, 38, 296, 353, 363, 530
MflI RGATCY 1 cut(s) 103
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 3 cut(s) 491, 587, 600
MlyI GAGTC 1 cut(s) 233
MroNI GCCGGC 1 cut(s) 65
MseI TTAA 1 cut(s) 437
MslI CAYNNNNRTG 2 cut(s) 141, 474
MspI CCGG 1 cut(s) 66
Mva1269I GAATGC 1 cut(s) 503
MvnI CGCG 1 cut(s) 414
MwoI GCNNNNNNNGC 1 cut(s) 62
NaeI GCCGGC 1 cut(s) 67
NdeII GATC 4 cut(s) 16, 93, 103, 340
NgoMIV GCCGGC 1 cut(s) 65
NlaIII CATG 1 cut(s) 26
NmeAIII GCCGAG 2 cut(s) 153, 301
NmuCI GTSAC 1 cut(s) 430
OliI CACNNNNGTG 2 cut(s) 141, 474
PaeR7I CTCGAG 1 cut(s) 217
PauI GCGCGC 1 cut(s) 412
PciSI GCTCTTC 1 cut(s) 309
PctI GAATGC 1 cut(s) 503
PdiI GCCGGC 1 cut(s) 67
PfeI GAWTC 3 cut(s) 354, 425, 616
PkrI GCNGC 2 cut(s) 58, 373
PleI GAGTC 1 cut(s) 233
PpsI GAGTC 1 cut(s) 233
PsiI TTATAA 1 cut(s) 486
PspPI GGNCC 1 cut(s) 280
PspXI VCTCGAGB 1 cut(s) 217
PsuI RGATCY 1 cut(s) 103
PteI GCGCGC 1 cut(s) 412
RsaI GTAC 2 cut(s) 75, 215
RsaNI GTAC 2 cut(s) 74, 214
RseI CAYNNNNRTG 2 cut(s) 141, 474
SapI GCTCTTC 1 cut(s) 309
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 2 cut(s) 57, 372
Sau3AI GATC 4 cut(s) 16, 93, 103, 340
Sau96I GGNCC 1 cut(s) 280
ScaI AGTACT 1 cut(s) 215
SchI GAGTC 1 cut(s) 233
SduI GDGCHC 1 cut(s) 46
SfaNI GCATC 5 cut(s) 52, 120, 278, 399, 551
Sfr274I CTCGAG 1 cut(s) 217
SlaI CTCGAG 1 cut(s) 217
SmiMI CAYNNNNRTG 2 cut(s) 141, 474
SmlI CTYRAG 2 cut(s) 217, 244
SmoI CTYRAG 2 cut(s) 217, 244
Sse9I AATT 3 cut(s) 491, 587, 600
SsiI CCGC 1 cut(s) 372
SspMI CTAG 2 cut(s) 552, 620
StyI CCWWGG 1 cut(s) 192
TaaI ACNGT 3 cut(s) 232, 295, 463
TaiI ACGT 1 cut(s) 437
TaqI TCGA 2 cut(s) 218, 585
TasI AATT 3 cut(s) 491, 587, 600
TatI WGTACW 1 cut(s) 213
TauI GCSGC 1 cut(s) 374
TfiI GAWTC 3 cut(s) 354, 425, 616
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TscAI CASTG 1 cut(s) 148
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 1 cut(s) 56
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 2 cut(s) 39, 594
TspRI CASTG 1 cut(s) 148
XapI RAATTY 1 cut(s) 600
XbaI TCTAGA 1 cut(s) 619
XhoI CTCGAG 1 cut(s) 217
XspI CTAG 2 cut(s) 552, 620
ZrmI AGTACT 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.