heat shock protein 70
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 232 |
| Acc65I | GGTACC | 1 cut(s) | 554 |
| AccB1I | GGYRCC | 1 cut(s) | 554 |
| AccI | GTMKAC | 2 cut(s) | 131, 256 |
| AccII | CGCG | 1 cut(s) | 176 |
| AciI | CCGC | 3 cut(s) | 174, 176, 663 |
| AcoI | YGGCCR | 2 cut(s) | 112, 371 |
| AcyI | GRCGYC | 1 cut(s) | 125 |
| AdeI | CACNNNGTG | 2 cut(s) | 715, 768 |
| AfaI | GTAC | 1 cut(s) | 556 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 369 |
| AgsI | TTSAA | 8 cut(s) | 207, 520, 598, 677, 683, 692, 729, 787 |
| AjnI | CCWGG | 2 cut(s) | 198, 368 |
| AluBI | AGCT | 5 cut(s) | 51, 193, 289, 338, 451 |
| AluI | AGCT | 5 cut(s) | 51, 193, 289, 338, 451 |
| Alw21I | GWGCWC | 1 cut(s) | 549 |
| Alw26I | GTCTC | 1 cut(s) | 478 |
| AoxI | GGCC | 2 cut(s) | 112, 371 |
| ApeKI | GCWGC | 1 cut(s) | 210 |
| Asp718I | GGTACC | 1 cut(s) | 554 |
| AspLEI | GCGC | 1 cut(s) | 626 |
| AsuHPI | GGTGA | 1 cut(s) | 614 |
| BaeGI | GKGCMC | 1 cut(s) | 764 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 538, 571 |
| BalI | TGGCCA | 2 cut(s) | 114, 373 |
| BanI | GGYRCC | 1 cut(s) | 554 |
| BauI | CACGAG | 1 cut(s) | 52 |
| BbsI | GAAGAC | 1 cut(s) | 338 |
| Bbv12I | GWGCWC | 1 cut(s) | 549 |
| BbvCI | CCTCAGC | 1 cut(s) | 531 |
| BbvI | GCAGC | 1 cut(s) | 222 |
| BccI | CCATC | 4 cut(s) | 175, 224, 310, 613 |
| BceAI | ACGGC | 3 cut(s) | 36, 112, 760 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 553, 587 |
| BciT130I | CCWGG | 2 cut(s) | 200, 370 |
| BclI | TGATCA | 2 cut(s) | 427, 502 |
| BcoDI | GTCTC | 1 cut(s) | 478 |
| BfaI | CTAG | 2 cut(s) | 290, 437 |
| BfmI | CTRYAG | 2 cut(s) | 132, 580 |
| BfoI | RGCGCY | 1 cut(s) | 627 |
| BfuAI | ACCTGC | 1 cut(s) | 232 |
| BisI | GCNGC | 1 cut(s) | 211 |
| BlsI | GCNGC | 1 cut(s) | 212 |
| Bme1390I | CCNGG | 2 cut(s) | 200, 370 |
| BmiI | GGNNCC | 1 cut(s) | 556 |
| BmrFI | CCNGG | 2 cut(s) | 200, 370 |
| BmsI | GCATC | 1 cut(s) | 394 |
| BpiI | GAAGAC | 1 cut(s) | 338 |
| Bpu10I | CCTNAGC | 1 cut(s) | 531 |
| BpuEI | CTTGAG | 1 cut(s) | 473 |
| Bsa29I | ATCGAT | 1 cut(s) | 33 |
| BsaBI | GATNNNNATC | 1 cut(s) | 485 |
| BsaHI | GRCGYC | 1 cut(s) | 125 |
| BsaJI | CCNNGG | 3 cut(s) | 174, 198, 404 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 369 |
| Bse3DI | GCAATG | 1 cut(s) | 676 |
| Bse8I | GATNNNNATC | 1 cut(s) | 485 |
| BseBI | CCWGG | 2 cut(s) | 200, 370 |
| BseCI | ATCGAT | 1 cut(s) | 33 |
| BseDI | CCNNGG | 3 cut(s) | 174, 198, 404 |
| BseJI | GATNNNNATC | 1 cut(s) | 485 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 369 |
| BseMI | GCAATG | 1 cut(s) | 676 |
| BseMII | CTCAG | 3 cut(s) | 292, 522, 534 |
| BseRI | GAGGAG | 1 cut(s) | 656 |
| BseSI | GKGCMC | 1 cut(s) | 764 |
| BseXI | GCAGC | 1 cut(s) | 222 |
| Bsh1236I | CGCG | 1 cut(s) | 176 |
| BshFI | GGCC | 2 cut(s) | 114, 373 |
| BshNI | GGYRCC | 1 cut(s) | 554 |
| BshVI | ATCGAT | 1 cut(s) | 33 |
| BsiHKAI | GWGCWC | 1 cut(s) | 549 |
| BslFI | GGGAC | 1 cut(s) | 83 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 369 |
| BsmAI | GTCTC | 1 cut(s) | 478 |
| BsmFI | GGGAC | 1 cut(s) | 83 |
| BsnI | GGCC | 2 cut(s) | 114, 373 |
| Bsp1286I | GDGCHC | 2 cut(s) | 549, 764 |
| Bsp143I | GATC | 4 cut(s) | 427, 480, 502, 694 |
| Bsp19I | CCATGG | 1 cut(s) | 404 |
| BspACI | CCGC | 3 cut(s) | 174, 176, 663 |
| BspANI | GGCC | 2 cut(s) | 114, 373 |
| BspCNI | CTCAG | 3 cut(s) | 291, 523, 535 |
| BspDI | ATCGAT | 1 cut(s) | 33 |
| BspFNI | CGCG | 1 cut(s) | 176 |
| BspLI | GGNNCC | 1 cut(s) | 556 |
| BspMI | ACCTGC | 1 cut(s) | 232 |
| BspT107I | GGYRCC | 1 cut(s) | 554 |
| BsrDI | GCAATG | 1 cut(s) | 676 |
| BssECI | CCNNGG | 3 cut(s) | 174, 198, 404 |
| BssMI | GATC | 4 cut(s) | 427, 480, 502, 694 |
| BssNI | GRCGYC | 1 cut(s) | 125 |
| BssSI | CACGAG | 1 cut(s) | 52 |
| BssT1I | CCWWGG | 1 cut(s) | 404 |
| Bst2BI | CACGAG | 1 cut(s) | 52 |
| Bst2UI | CCWGG | 2 cut(s) | 200, 370 |
| Bst4CI | ACNGT | 3 cut(s) | 136, 581, 602 |
| BstACI | GRCGYC | 1 cut(s) | 125 |
| BstC8I | GCNNGC | 4 cut(s) | 195, 566, 657, 661 |
| BstDEI | CTNAG | 4 cut(s) | 278, 531, 543, 627 |
| BstDSI | CCRYGG | 2 cut(s) | 174, 404 |
| BstFNI | CGCG | 1 cut(s) | 176 |
| BstH2I | RGCGCY | 1 cut(s) | 627 |
| BstHHI | GCGC | 1 cut(s) | 626 |
| BstKTI | GATC | 4 cut(s) | 430, 483, 505, 697 |
| BstMAI | GTCTC | 1 cut(s) | 478 |
| BstMBI | GATC | 4 cut(s) | 427, 480, 502, 694 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 48, 632, 652 |
| BstNI | CCWGG | 2 cut(s) | 200, 370 |
| BstNSI | RCATGY | 2 cut(s) | 568, 659 |
| BstSCI | CCNGG | 2 cut(s) | 198, 368 |
| BstSFI | CTRYAG | 2 cut(s) | 132, 580 |
| BstSLI | GKGCMC | 1 cut(s) | 764 |
| BstUI | CGCG | 1 cut(s) | 176 |
| BstV1I | GCAGC | 1 cut(s) | 222 |
| BstV2I | GAAGAC | 1 cut(s) | 338 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 381 |
| Bsu15I | ATCGAT | 1 cut(s) | 33 |
| BsuRI | GGCC | 2 cut(s) | 114, 373 |
| BsuTUI | ATCGAT | 1 cut(s) | 33 |
| BtgI | CCRYGG | 2 cut(s) | 174, 404 |
| BveI | ACCTGC | 1 cut(s) | 232 |
| Cac8I | GCNNGC | 4 cut(s) | 195, 566, 657, 661 |
| CfoI | GCGC | 1 cut(s) | 626 |
| Cfr42I | CCGCGG | 1 cut(s) | 177 |
| ClaI | ATCGAT | 1 cut(s) | 33 |
| CseI | GACGC | 1 cut(s) | 133 |
| Csp6I | GTAC | 1 cut(s) | 555 |
| CviAII | CATG | 6 cut(s) | 110, 349, 405, 565, 656, 780 |
| CviQI | GTAC | 1 cut(s) | 555 |
| DdeI | CTNAG | 4 cut(s) | 278, 531, 543, 627 |
| DpnI | GATC | 4 cut(s) | 429, 482, 504, 696 |
| DpnII | GATC | 4 cut(s) | 427, 480, 502, 694 |
| DraIII | CACNNNGTG | 2 cut(s) | 715, 768 |
| EaeI | YGGCCR | 2 cut(s) | 112, 371 |
| EciI | GGCGGA | 1 cut(s) | 678 |
| Eco130I | CCWWGG | 1 cut(s) | 404 |
| EcoRII | CCWGG | 2 cut(s) | 198, 368 |
| EcoT14I | CCWWGG | 1 cut(s) | 404 |
| ErhI | CCWWGG | 1 cut(s) | 404 |
| FaeI | CATG | 6 cut(s) | 113, 352, 408, 568, 659, 783 |
| FaqI | GGGAC | 1 cut(s) | 83 |
| FatI | CATG | 6 cut(s) | 109, 348, 404, 564, 655, 779 |
| FbaI | TGATCA | 2 cut(s) | 427, 502 |
| FblI | GTMKAC | 2 cut(s) | 131, 256 |
| Fnu4HI | GCNGC | 1 cut(s) | 211 |
| Fsp4HI | GCNGC | 1 cut(s) | 211 |
| FspBI | CTAG | 2 cut(s) | 290, 437 |
| GlaI | GCGC | 1 cut(s) | 625 |
| GluI | GCNGC | 1 cut(s) | 211 |
| HaeII | RGCGCY | 1 cut(s) | 627 |
| HaeIII | GGCC | 2 cut(s) | 114, 373 |
| HgaI | GACGC | 1 cut(s) | 133 |
| HhaI | GCGC | 1 cut(s) | 626 |
| Hin1I | GRCGYC | 1 cut(s) | 125 |
| Hin1II | CATG | 6 cut(s) | 113, 352, 408, 568, 659, 783 |
| Hin6I | GCGC | 1 cut(s) | 624 |
| HinP1I | GCGC | 1 cut(s) | 624 |
| HincII | GTYRAC | 1 cut(s) | 577 |
| HindII | GTYRAC | 1 cut(s) | 577 |
| HinfI | GANTC | 3 cut(s) | 71, 203, 460 |
| HphI | GGTGA | 1 cut(s) | 614 |
| Hpy166II | GTNNAC | 3 cut(s) | 132, 257, 577 |
| Hpy188I | TCNGA | 2 cut(s) | 507, 544 |
| Hpy188III | TCNNGA | 1 cut(s) | 389 |
| Hpy8I | GTNNAC | 3 cut(s) | 132, 257, 577 |
| Hpy99I | CGWCG | 1 cut(s) | 127 |
| HpyAV | CCTTC | 1 cut(s) | 413 |
| HpyCH4III | ACNGT | 3 cut(s) | 136, 581, 602 |
| HpyCH4V | TGCA | 2 cut(s) | 241, 748 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 48, 632, 652 |
| HpyF3I | CTNAG | 4 cut(s) | 278, 531, 543, 627 |
| Hsp92I | GRCGYC | 1 cut(s) | 125 |
| Hsp92II | CATG | 6 cut(s) | 113, 352, 408, 568, 659, 783 |
| HspAI | GCGC | 1 cut(s) | 624 |
| KpnI | GGTACC | 1 cut(s) | 558 |
| Ksp22I | TGATCA | 2 cut(s) | 427, 502 |
| KspI | CCGCGG | 1 cut(s) | 177 |
| Kzo9I | GATC | 4 cut(s) | 427, 480, 502, 694 |
| LmnI | GCTCC | 2 cut(s) | 286, 643 |
| Lsp1109I | GCAGC | 1 cut(s) | 222 |
| LweI | GCATC | 1 cut(s) | 394 |
| MaeI | CTAG | 2 cut(s) | 290, 437 |
| MaeIII | GTNAC | 2 cut(s) | 169, 523 |
| MalI | GATC | 4 cut(s) | 429, 482, 504, 696 |
| MboI | GATC | 4 cut(s) | 427, 480, 502, 694 |
| MboII | GAAGA | 3 cut(s) | 343, 425, 704 |
| MfeI | CAATTG | 1 cut(s) | 24 |
| MhlI | GDGCHC | 2 cut(s) | 549, 764 |
| MlsI | TGGCCA | 2 cut(s) | 114, 373 |
| MluCI | AATT | 3 cut(s) | 24, 647, 755 |
| MluNI | TGGCCA | 2 cut(s) | 114, 373 |
| MlyI | GAGTC | 1 cut(s) | 65 |
| MmeI | TCCRAC | 1 cut(s) | 368 |
| MnlI | CCTC | 8 cut(s) | 85, 178, 285, 321, 448, 526, 582, 634 |
| Mox20I | TGGCCA | 2 cut(s) | 114, 373 |
| MscI | TGGCCA | 2 cut(s) | 114, 373 |
| MseI | TTAA | 2 cut(s) | 650, 720 |
| MslI | CAYNNNNRTG | 1 cut(s) | 269 |
| Msp20I | TGGCCA | 2 cut(s) | 114, 373 |
| MspA1I | CMGCKG | 1 cut(s) | 176 |
| MspR9I | CCNGG | 2 cut(s) | 200, 370 |
| MunI | CAATTG | 1 cut(s) | 24 |
| MvaI | CCWGG | 2 cut(s) | 200, 370 |
| MvnI | CGCG | 1 cut(s) | 176 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 48, 632, 652 |
| NcoI | CCATGG | 1 cut(s) | 404 |
| NdeII | GATC | 4 cut(s) | 427, 480, 502, 694 |
| NlaIII | CATG | 6 cut(s) | 113, 352, 408, 568, 659, 783 |
| NlaIV | GGNNCC | 1 cut(s) | 556 |
| NspI | RCATGY | 2 cut(s) | 568, 659 |
| PaeI | GCATGC | 2 cut(s) | 568, 659 |
| PfeI | GAWTC | 2 cut(s) | 203, 460 |
| PflFI | GACNNNGTC | 1 cut(s) | 127 |
| PkrI | GCNGC | 1 cut(s) | 212 |
| PleI | GAGTC | 1 cut(s) | 65 |
| PpsI | GAGTC | 1 cut(s) | 65 |
| Psp6I | CCWGG | 2 cut(s) | 198, 368 |
| PspGI | CCWGG | 2 cut(s) | 198, 368 |
| PspN4I | GGNNCC | 1 cut(s) | 556 |
| PsyI | GACNNNGTC | 1 cut(s) | 127 |
| RsaI | GTAC | 1 cut(s) | 556 |
| RsaNI | GTAC | 1 cut(s) | 555 |
| RseI | CAYNNNNRTG | 1 cut(s) | 269 |
| SacII | CCGCGG | 1 cut(s) | 177 |
| SaqAI | TTAA | 2 cut(s) | 650, 720 |
| SatI | GCNGC | 1 cut(s) | 211 |
| Sau3AI | GATC | 4 cut(s) | 427, 480, 502, 694 |
| SchI | GAGTC | 1 cut(s) | 65 |
| ScrFI | CCNGG | 2 cut(s) | 200, 370 |
| SduI | GDGCHC | 2 cut(s) | 549, 764 |
| SfaNI | GCATC | 1 cut(s) | 394 |
| SfcI | CTRYAG | 2 cut(s) | 132, 580 |
| Sfr303I | CCGCGG | 1 cut(s) | 177 |
| SgrBI | CCGCGG | 1 cut(s) | 177 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 269 |
| SmlI | CTYRAG | 1 cut(s) | 452 |
| SmoI | CTYRAG | 1 cut(s) | 452 |
| SphI | GCATGC | 2 cut(s) | 568, 659 |
| Sse9I | AATT | 3 cut(s) | 24, 647, 755 |
| SsiI | CCGC | 3 cut(s) | 174, 176, 663 |
| SspMI | CTAG | 2 cut(s) | 290, 437 |
| StyD4I | CCNGG | 2 cut(s) | 198, 368 |
| StyI | CCWWGG | 1 cut(s) | 404 |
| TaaI | ACNGT | 3 cut(s) | 136, 581, 602 |
| TaqI | TCGA | 1 cut(s) | 33 |
| TasI | AATT | 3 cut(s) | 24, 647, 755 |
| TfiI | GAWTC | 2 cut(s) | 203, 460 |
| Tru1I | TTAA | 2 cut(s) | 650, 720 |
| Tru9I | TTAA | 2 cut(s) | 650, 720 |
| TseI | GCWGC | 1 cut(s) | 210 |
| TspDTI | ATGAA | 3 cut(s) | 365, 432, 767 |
| Tth111I | GACNNNGTC | 1 cut(s) | 127 |
| XceI | RCATGY | 2 cut(s) | 568, 659 |
| XmiI | GTMKAC | 2 cut(s) | 131, 256 |
| XspI | CTAG | 2 cut(s) | 290, 437 |