Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr7C

Orthogroup ID

OG0000017

Physical Location & Seq

Genomic Coordinates Forward (+)

30155017 .. 30164312

Gene Structure 9296 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh7CG295300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 270 bp

ATGTCTCAAACTAGAGTAGAAGCATTTGCTATCAATGCCAGCAATCTTAGGAGAGTCGTCTCAAAATTCCGGTTGCATTTTATTCTGAACCTGCCTGACCTAAAGCATTCTCCATTGGAGCGTATGGCAGCTACTTCCTTACAAACAGCGTGGCGTGAAAGAGAAAATCGCAGCAGTGGATGGAACAAATTGAAGGAATTACTCAACTATTCATCAGAAAGGCACCTCATTGAGATCTTCAAACATATATTACATATGCAAAACATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0006950 GO:0006952 GO:0008150 GO:0008324 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0043207 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0050896 GO:0051179 GO:0051234 GO:0051704 GO:0051707 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

89

Amino Acids

10.57

Weight (kDa)

10.55

Isoelectric Point (pI)

70.66

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000017)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g07351 FvH4_1g11800 FvH4_1g11800 FvH4_1g11800 FvH4_1g11800 FvH4_1g11800 FvH4_1g22250 FvH4_1g22250 FvH4_1g22250 FvH4_1g22250 FvH4_1g22250 FvH4_1g22250 FvH4_1g22250 FvH4_1g22250 FvH4_1g22250 FvH4_1g22250 FvH4_1g22250 FvH4_4g14360 FvH4_6g13561 FvH4_6g13562 FvH4_6g13580 FvH4_6g13590 FvH4_6g13590 FvH4_6g13590 FvH4_6g13731 FvH4_6g13770 FvH4_6g13800 FvH4_6g13800 FvH4_6g13860 FvH4_6g13870 FvH4_6g13870 FvH4_6g13870 FvH4_6g13870 FvH4_6g13870 FvH4_6g13890 FvH4_6g33801 FvH4_7g13020 FvH4_7g13270 FvH4_7g13270 FvH4_7g13280 FvH4_7g13280 FvH4_7g13280 FvH4_7g13280 FvH4_7g13280 FvH4_7g13280 FvH4_7g13280 FvH4_7g13280 FvH4_7g14580 FvH4_7g14580 FvH4_7g14580 FvH4_7g14600 FvH4_7g14600 FvH4_7g20030 FvH4_7g20510 FvH4_7g20510 FvH4_7g20510 FvH4_7g20670 FvH4_7g20670 FvH4_7g20670 FvH4_7g20670 FvH4_7g20690 FvH4_7g20690 FvH4_7g31930 FvH4_7g31930 FvH4_7g31940 FvH4_7g31940
malus_domestica	MD01G1115700.v1.1 MD01G1115800.v1.1 MD01G1116600.v1.1 MD01G1116700.v1.1 MD01G1117000.v1.1 MD01G1225000.v1.1 MD02G1077900.v1.1 MD02G1078000.v1.1 MD02G1078200.v1.1 MD02G1078400.v1.1 MD02G1084300.v1.1 MD04G1004700.v1.1 MD04G1123100.v1.1 MD04G1123200.v1.1 MD04G1128000.v1.1 MD04G1128400.v1.1 MD05G1299700.v1.1 MD05G1300000.v1.1 MD07G1184000.v1.1 MD07G1292300.v1.1 MD08G1214800.v1.1
prunus_persica	Prupe.1G205100_v2.0.a1 Prupe.1G205100_v2.0.a1 Prupe.1G205200_v2.0.a1 Prupe.2G222300_v2.0.a1 Prupe.2G223300_v2.0.a1 Prupe.2G223500_v2.0.a1 Prupe.2G223500_v2.0.a1 Prupe.2G223500_v2.0.a1 Prupe.2G223500_v2.0.a1 Prupe.2G223500_v2.0.a1 Prupe.2G223600_v2.0.a1 Prupe.2G223600_v2.0.a1 Prupe.2G223600_v2.0.a1 Prupe.2G223700_v2.0.a1 Prupe.2G223700_v2.0.a1 Prupe.2G223700_v2.0.a1 Prupe.2G223700_v2.0.a1 Prupe.2G223800_v2.0.a1 Prupe.2G223900_v2.0.a1 Prupe.2G224000_v2.0.a1 Prupe.5G162000_v2.0.a1 Prupe.6G254400_v2.0.a1 Prupe.6G254400_v2.0.a1 Prupe.6G254400_v2.0.a1 Prupe.6G254400_v2.0.a1 Prupe.6G254400_v2.0.a1 Prupe.6G291000_v2.0.a1 Prupe.7G057700_v2.0.a1 Prupe.7G057700_v2.0.a1 Prupe.7G057700_v2.0.a1 Prupe.7G057700_v2.0.a1 Prupe.7G057700_v2.0.a1 Prupe.7G057700_v2.0.a1 Prupe.7G057700_v2.0.a1 Prupe.7G057700_v2.0.a1 Prupe.7G073200_v2.0.a1 Prupe.7G073200_v2.0.a1 Prupe.7G073200_v2.0.a1 Prupe.7G073200_v2.0.a1 Prupe.7G073200_v2.0.a1 Prupe.7G073200_v2.0.a1 Prupe.7G073200_v2.0.a1 Prupe.7G210400_v2.0.a1 Prupe.7G210600_v2.0.a1 Prupe.7G210700_v2.0.a1 Prupe.7G210800_v2.0.a1 Prupe.7G210900_v2.0.a1 Prupe.8G097600_v2.0.a1 Prupe.8G097800_v2.0.a1 Prupe.8G097800_v2.0.a1
pyrus_communis	pycom01g14430 pycom01g14440 pycom01g23130 pycom01g23160 pycom01g23330 pycom02g06210 pycom02g06220 pycom02g06250 pycom02g06270 pycom02g06710 pycom03g05360 pycom03g05380 pycom04g11470 pycom04g11480 pycom04g11520 pycom04g11560 pycom04g11600 pycom04g11610 pycom04g11650 pycom04g11660 pycom04g11670 pycom05g27790 pycom07g17630 pycom08g20960 pycom126g00020 pycom126g00030
rosa_chinensis	RchiOBHm_Chr1g0318251 RchiOBHm_Chr1g0318731 RchiOBHm_Chr1g0339101 RchiOBHm_Chr1g0339111 RchiOBHm_Chr1g0347151 RchiOBHm_Chr1g0347161 RchiOBHm_Chr1g0347221 RchiOBHm_Chr1g0352401 RchiOBHm_Chr1g0352441 RchiOBHm_Chr1g0352461 RchiOBHm_Chr1g0356131 RchiOBHm_Chr1g0363991 RchiOBHm_Chr1g0364791 RchiOBHm_Chr1g0364801 RchiOBHm_Chr1g0364811 RchiOBHm_Chr1g0364851 RchiOBHm_Chr1g0364951 RchiOBHm_Chr1g0370821 RchiOBHm_Chr1g0381221 RchiOBHm_Chr1g0381231 RchiOBHm_Chr1g0381261 RchiOBHm_Chr1g0381271 RchiOBHm_Chr1g0381341 RchiOBHm_Chr1g0381351 RchiOBHm_Chr1g0381391 RchiOBHm_Chr1g0381801 RchiOBHm_Chr1g0381821 RchiOBHm_Chr2g0093241 RchiOBHm_Chr2g0116391 RchiOBHm_Chr3g0466441 RchiOBHm_Chr3g0466631 RchiOBHm_Chr3g0466651 RchiOBHm_Chr3g0496921 RchiOBHm_Chr4g0400441 RchiOBHm_Chr4g0400501 RchiOBHm_Chr4g0400511 RchiOBHm_Chr4g0400691 RchiOBHm_Chr4g0400701 RchiOBHm_Chr4g0404331 RchiOBHm_Chr5g0052101 RchiOBHm_Chr5g0052111 RchiOBHm_Chr5g0052171 RchiOBHm_Chr5g0052181 RchiOBHm_Chr6g0264161 RchiOBHm_Chr6g0265551 RchiOBHm_Chr7g0213571 RchiOBHm_Chr7g0213581
rosa_laevigata	RLG00000002796 RLG00000003261 RLG00000007935 RLG00000007936 RLG00000009187 RLG00000014179 RLG00000016381 RLG00000018265 RLG00000024558 RLG00000024574 RLG00000026232 RLG00000026234 RLG00000026236 RLG00000026237 RLG00000027459 RLG00000027461 RLG00000027463 RLG00000027530 RLG00000028103 RLG00000028304 RLG00000028333 RLG00000029214 RLG00000029848 RLG00000029951 RLG00000030115 RLG00000030552 RLG00000033668 RLG00000034840 RLG00000034841 RLG00000034842 RLG00000035830
rosa_multiflora	Rmu_sc0000654.1_g000004 Rmu_sc0000675.1_g000025 Rmu_sc0001075.1_g000002 Rmu_sc0001726.1_g000004 Rmu_sc0001772.1_g000061 Rmu_sc0001772.1_g000065 Rmu_sc0002067.1_g000002 Rmu_sc0002183.1_g000001 Rmu_sc0002183.1_g000006 Rmu_sc0002244.1_g000003 Rmu_sc0002313.1_g000004 Rmu_sc0002319.1_g000004 Rmu_sc0002539.1_g000085 Rmu_sc0002636.1_g000004 Rmu_sc0003229.1_g000007 Rmu_sc0003313.1_g000020 Rmu_sc0003546.1_g000007 Rmu_sc0003724.1_g000015 Rmu_sc0003936.1_g000029 Rmu_sc0004191.1_g000067 Rmu_sc0004525.1_g000012 Rmu_sc0005122.1_g000009 Rmu_sc0005255.1_g000040 Rmu_sc0005338.1_g000016 Rmu_sc0005584.1_g000008 Rmu_sc0006460.1_g000013 Rmu_sc0006872.1_g000011 Rmu_sc0006872.1_g000015 Rmu_sc0006872.1_g000016 Rmu_sc0006952.1_g000013 Rmu_sc0007342.1_g000005 Rmu_sc0007342.1_g000006 Rmu_sc0008418.1_g000003 Rmu_sc0008418.1_g000006 Rmu_sc0008636.1_g000007 Rmu_sc0008636.1_g000008 Rmu_sc0009205.1_g000014 Rmu_sc0009365.1_g000014 Rmu_sc0009917.1_g000002 Rmu_sc0019289.1_g000003 Rmu_ssc0000098.1_g000011 Rmu_ssc0000098.1_g000012 Rmu_ssc0000167.1_g000018 Rmu_ssc0000167.1_g000024 Rmu_ssc0000326.1_g000015
rosa_roxburghii	Rroxscaffold_166G00440780 Rroxscaffold_176G00431500 Rroxscaffold_1G00015080 Rroxscaffold_1G00027880 Rroxscaffold_1G00027890 Rroxscaffold_1G00027920 Rroxscaffold_2G00127250 Rroxscaffold_2G00148380 Rroxscaffold_3G00219450 Rroxscaffold_3G00232170 Rroxscaffold_3G00232210 Rroxscaffold_3G00242230 Rroxscaffold_3G00242240 Rroxscaffold_3G00244790 Rroxscaffold_3G00245790 Rroxscaffold_4G00278400 Rroxscaffold_4G00278430 Rroxscaffold_4G00278670 Rroxscaffold_4G00278680 Rroxscaffold_4G00278690 Rroxscaffold_4G00278700 Rroxscaffold_4G00278720 Rroxscaffold_4G00291690 Rroxscaffold_4G00292070 Rroxscaffold_4G00292090 Rroxscaffold_4G00292770 Rroxscaffold_4G00302240 Rroxscaffold_4G00302860 Rroxscaffold_4G00311320 Rroxscaffold_4G00311330 Rroxscaffold_4G00320570 Rroxscaffold_4G00322050 Rroxscaffold_4G00329480 Rroxscaffold_4G00329730 Rroxscaffold_5G00345500 Rroxscaffold_5G00345510 Rroxscaffold_5G00360830 Rroxscaffold_5G00360840 Rroxscaffold_6G00393450 Rroxscaffold_6G00404030 Rroxscaffold_6G00414220 Rroxscaffold_6G00414230 Rroxscaffold_6G00414280 Rroxscaffold_6G00414290 Rroxscaffold_6G00414350 Rroxscaffold_6G00414470 Rroxscaffold_7G00202570 Rroxscaffold_7G00203460 Rroxscaffold_7G00203470
rosa_rugosa	Rorug01G0020500 Rorug01G0020600 Rorug01G0020700 Rorug01G0143700.1 Rorug01G0225700 Rorug01G0228500 Rorug01G0228600 Rorug01G0228600 Rorug01G0228700 Rorug01G0228800 Rorug01G0228800 Rorug01G0229000 Rorug01G0229300 Rorug01G0229400 Rorug01G0229600 Rorug01G0229700 Rorug01G0232200 Rorug01G0232600 Rorug01G0232600 Rorug01G0311900 Rorug01G0311900 Rorug01G0318400 Rorug01G0318500 Rorug01G0318500 Rorug01G0318500 Rorug01G0427900 Rorug01G0427900 Rorug01G0428000 Rorug01G0428200 Rorug01G0428300 Rorug01G0428400 Rorug01G0431500 Rorug01G0431600 Rorug01G0431600 Rorug02G0033800 Rorug02G0206300 Rorug03G0081900 Rorug03G0083200 Rorug03G0083200 Rorug03G0083300 Rorug04G0025100 Rorug04G0025100 Rorug04G0025200 Rorug04G0025300 Rorug04G0058600 Rorug04G0058700 Rorug04G0058800 Rorug04G0098400 Rorug04G0098500 Rorug05G0274500 Rorug05G0274600 Rorug05G0274800 Rorug06G0008500 Rorug06G0009100 Rorug06G0018000
rosa_samantha	Rh1AG031700 Rh1AG036100 Rh1AG089800 Rh1AG239800 Rh1AG239900 Rh1AG242000 Rh1AG242300 Rh1AG245600 Rh1AG262600 Rh1AG319700 Rh1AG319800 Rh1AG319900 Rh1AG327200 Rh1AG451100 Rh1AG451200 Rh1AG451300 Rh1AG451600 Rh1AG451700 Rh1BG027200 Rh1BG071600 Rh1BG090700 Rh1BG211000 Rh1BG213000 Rh1BG213500 Rh1BG282800 Rh1BG288400 Rh1BG408100 Rh1BG408200 Rh1BG408400 Rh1BG408700 Rh1CG026300 Rh1CG028300 Rh1CG224100 Rh1CG226000 Rh1CG226100 Rh1CG229300 Rh1CG229400 Rh1CG245900 Rh1CG299200 Rh1CG299400 Rh1CG299500 Rh1CG303300 Rh1CG305100 Rh1CG422500 Rh1CG422600 Rh1CG422700 Rh1CG422800 Rh1CG422900 Rh1CG423100 Rh1CG423300 Rh1CG423400 Rh1CG426300 Rh1CG426600 Rh1DG239300 Rh1DG239600 Rh1DG258300 Rh1DG314200 Rh1DG314300 Rh1DG314400 Rh1DG320000 Rh1DG439200 Rh1DG439400 Rh1DG439500 Rh1DG439700 Rh1DG440000 Rh1DG443800 Rh2AG261800 Rh2BG080900 Rh2CG083300 Rh2DG078900 Rh2DG270000 Rh2DG270100 Rh3AG128400 Rh3AG130100 Rh3AG130500 Rh3AG332500 Rh3AG332600 Rh3AG332800 Rh3BG149700 Rh3BG151500 Rh3CG151400 Rh3CG354500 Rh3CG366800 Rh3DG150500 Rh3DG152200 Rh4AG101400 Rh4AG102900 Rh4AG148300 Rh4AG148600 Rh4BG098000 Rh4CG111100 Rh4CG111300 Rh4CG155100 Rh4DG094800 Rh5AG213600 Rh5AG346900 Rh5AG347000 Rh5BG357500 Rh5BG357700 Rh5BG485400 Rh5CG383100 Rh5CG383200 Rh5CG383300 Rh5CG509900 Rh5DG373000 Rh5DG373200 Rh6BG054300 Rh6BG126300 Rh6CG125100 Rh6CG185300 Rh6DG177000 Rh7AG276100 Rh7AG276200 Rh7BG230500 Rh7BG270500 Rh7BG364000 Rh7CG250100 Rh7CG295200 Rh7CG295300 Rh7DG389700
rosa_wichuraiana	Rw0G001190 Rw0G010680 Rw0G013360 Rw0G014310 Rw0G014890 Rw0G020310 Rw0G021350 Rw0G022510 Rw1G003360 Rw1G004330 Rw1G006930 Rw1G008310 Rw1G021090 Rw1G021150 Rw1G021210 Rw1G023200 Rw1G023210 Rw1G028340 Rw1G028350 Rw1G028990 Rw1G032510 Rw1G039370 Rw1G039400 Rw1G039410 Rw1G039420 Rw1G039430 Rw2G006700 Rw2G020550 Rw2G020580 Rw3G012130 Rw3G012300 Rw3G012310 Rw3G028220 Rw3G029230 Rw5G021600 Rw5G032790 Rw6G011240 Rw7G027670 Rw7G032780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	99
AccB1I	GGYRCC	1 cut(s)	222
AcsI	RAATTY	1 cut(s)	65
AgsI	TTSAA	2 cut(s)	193, 241
AluBI	AGCT	1 cut(s)	131
AluI	AGCT	1 cut(s)	131
Alw26I	GTCTC	2 cut(s)	9, 64
ApeKI	GCWGC	2 cut(s)	128, 171
ApoI	RAATTY	1 cut(s)	65
BanI	GGYRCC	1 cut(s)	222
BbvI	GCAGC	2 cut(s)	140, 183
BccI	CCATC	1 cut(s)	174
BcoDI	GTCTC	2 cut(s)	9, 64
BfaI	CTAG	1 cut(s)	12
BfuAI	ACCTGC	1 cut(s)	99
BglII	AGATCT	1 cut(s)	234
BisI	GCNGC	2 cut(s)	129, 172
BlsI	GCNGC	2 cut(s)	130, 173
BmiI	GGNNCC	1 cut(s)	224
BsaWI	WCCGGW	1 cut(s)	69
BseGI	GGATG	1 cut(s)	185
BseXI	GCAGC	2 cut(s)	140, 183
BshNI	GGYRCC	1 cut(s)	222
BsiSI	CCGG	1 cut(s)	70
BsmAI	GTCTC	2 cut(s)	9, 64
BsmBI	CGTCTC	1 cut(s)	64
BsmI	GAATGC	1 cut(s)	106
Bsp143I	GATC	1 cut(s)	234
BspLI	GGNNCC	1 cut(s)	224
BspMI	ACCTGC	1 cut(s)	99
BspT107I	GGYRCC	1 cut(s)	222
BssMI	GATC	1 cut(s)	234
BstC8I	GCNNGC	1 cut(s)	40
BstDEI	CTNAG	1 cut(s)	47
BstF5I	GGATG	1 cut(s)	185
BstKTI	GATC	1 cut(s)	237
BstMAI	GTCTC	2 cut(s)	9, 64
BstMBI	GATC	1 cut(s)	234
BstMWI	GCNNNNNNNGC	1 cut(s)	35
BstV1I	GCAGC	2 cut(s)	140, 183
BstX2I	RGATCY	1 cut(s)	234
BstYI	RGATCY	1 cut(s)	234
BtsCI	GGATG	1 cut(s)	185
BtsI	GCAGTG	1 cut(s)	181
BtsIMutI	CAGTG	1 cut(s)	181
BveI	ACCTGC	1 cut(s)	99
Cac8I	GCNNGC	1 cut(s)	40
CspCI	CAANNNNNGTGG	2 cut(s)	131, 166
CviJI	RGCY	1 cut(s)	131
CviKI_1	RGCY	1 cut(s)	131
DdeI	CTNAG	1 cut(s)	47
DpnI	GATC	1 cut(s)	236
DpnII	GATC	1 cut(s)	234
Esp3I	CGTCTC	1 cut(s)	64
FaiI	YATR	5 cut(s)	125, 246, 248, 255, 257
FauNDI	CATATG	1 cut(s)	255
Fnu4HI	GCNGC	2 cut(s)	129, 172
FokI	GGATG	1 cut(s)	192
Fsp4HI	GCNGC	2 cut(s)	129, 172
FspBI	CTAG	1 cut(s)	12
GluI	GCNGC	2 cut(s)	129, 172
HapII	CCGG	1 cut(s)	70
HinfI	GANTC	1 cut(s)	54
HpaII	CCGG	1 cut(s)	70
Hpy188I	TCNGA	2 cut(s)	87, 217
HpyAV	CCTTC	1 cut(s)	187
HpyCH4V	TGCA	2 cut(s)	76, 259
HpyF10VI	GCNNNNNNNGC	1 cut(s)	35
HpyF3I	CTNAG	1 cut(s)	47
Kzo9I	GATC	1 cut(s)	234
LmnI	GCTCC	1 cut(s)	118
LpnPI	CCDG	4 cut(s)	52, 83, 104, 108
Lsp1109I	GCAGC	2 cut(s)	140, 183
MaeI	CTAG	1 cut(s)	12
MalI	GATC	1 cut(s)	236
MboI	GATC	1 cut(s)	234
MboII	GAAGA	1 cut(s)	229
MflI	RGATCY	1 cut(s)	234
MluCI	AATT	3 cut(s)	65, 188, 197
MlyI	GAGTC	1 cut(s)	63
MnlI	CCTC	1 cut(s)	236
MspI	CCGG	1 cut(s)	70
Mva1269I	GAATGC	1 cut(s)	106
MwoI	GCNNNNNNNGC	1 cut(s)	35
NdeI	CATATG	1 cut(s)	255
NdeII	GATC	1 cut(s)	234
NlaIV	GGNNCC	1 cut(s)	224
PctI	GAATGC	1 cut(s)	106
PkrI	GCNGC	2 cut(s)	130, 173
PleI	GAGTC	1 cut(s)	62
PpsI	GAGTC	1 cut(s)	62
PspN4I	GGNNCC	1 cut(s)	224
PsuI	RGATCY	1 cut(s)	234
SatI	GCNGC	2 cut(s)	129, 172
Sau3AI	GATC	1 cut(s)	234
SchI	GAGTC	1 cut(s)	63
SetI	ASST	4 cut(s)	93, 102, 133, 228
SgeI	CNNG	7 cut(s)	24, 51, 82, 103, 107, 162, 167
Sse9I	AATT	3 cut(s)	65, 188, 197
SspMI	CTAG	1 cut(s)	12
TasI	AATT	3 cut(s)	65, 188, 197
TscAI	CASTG	1 cut(s)	181
TseI	GCWGC	2 cut(s)	128, 171
TspDTI	ATGAA	1 cut(s)	201
TspRI	CASTG	1 cut(s)	181
XapI	RAATTY	1 cut(s)	65
XspI	CTAG	1 cut(s)	12

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.