AT4G34880

amidase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000427
Physical Location & Seq
Forward (+)
16615391 .. 16617586
2196 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G34880.1

Sequence Viewer

Length: 1401 bp
ATGGTTGCTCTGCGTTTCTTCTTCTTCTCATCTCTTCTTATTCTTCTTATTCTTTCTCAAGCTCTTATTATGTCAGTTGGATCTGCCTCTCAGATCAGGCTTAGCTCAACGTTCTCGATCCAAGAAGCTACCATTGAAGACATTAGAGTTGCTTTCAACGAGAAACGACTAACATCAAAGCAGCTTGTGGAGTTGTATCTCGAAGCAATCTCCAAACTTAATCCAATACTCCACGCAGTCATCGAGACGAATCCTGATGCGTTGATCCAAGCAGAGATCGCAGACAGAGAACGTGACCTTAAAAACACCACTAAGCTTCCTATCTTGCACGGTGTTCCGGTTCTTCTCAAGGATTCCATATCTACCAAGGACAAGCTTAACACCACGGCTGGCTCGTTTGCCTTGCTTGGTTCGGTCGTGGCTCGAGACGCGGGTGTTGTTAAAAGACTGAGAGAATCTGGTGCTGTGATCTTGGGCAAAGCCAGTTTAAGTGAATGGGCTCATTTTCGATCTTTCTCCATCCCAGATGGTTGGAGCGCACCAAGTCAGAACTCTGTGGTCGGGATTAAACCGAGTGTCGGACTCACGAGCCGAGCCGGGGTAGTTCCTATCTCCTTGAGACAGGACAGTATTGGGCCGATTTGTAGGACAGTGTCGGATGCTGTTCATCTCCTTGATGCTATTGTGGGTTATGATCCATTGGACGAGGCCACGAAAACTGCGTCCGAGTTCATACCTGAAGGCGGTTACAAGCAGTTTCTGACAACTAGTGGCCTCAAAGGTAAGAGATTAGGGATTGTAATGAAGCATTCTTCTCTTCTTGATCATCACATCAAAACATTAAGACGAGAAGGCGCTATTGTCATTAACAATCTGACGATACCAAACATCGAAGTCATTGTGGGTGGGACTGATAGTGGTGAAGAGATAGCACTTTTGGCTGAGTTCAAGATGTCTCTCAATGCATATCTTAAAGAGCTTGTGAAATCACCAGTCCGGTCCTTAGCAGATGTTATTGCCTACAACGAGGAATTCGCTGAACAAGAAAAAGTGAAAGAATGGGGACAAGAGGTGTTCCTTACAGCAGAAGCCACTAGTGGAATGGGTGAGAAAGAGAAAACAGCGTTGCAGAAAATGAAAGAACTATCGAGGAATGGAATCGAAAAGCTAATTGAAGAGAACAAGTTAGATGCAATAGTGACACTTGGTTCAGATTTGAGCTCTGTCCTAGCCATTGGAGGGTATCCAGGAATCAATGTCCCTGCAGGATACGATAGTGGAGGAGTTCCATATGGGATTAGCTTTGGAGGACTGAGATTCTCAGAGCCAAAGCTCATTGAGATTGCTTTTGCCTTTGAACAAGCAACTCTTATTAGAAAGCCACCCAAGTTCATAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

466

Amino Acids

50.5

Weight (kDa)

6.24

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 62 - 170 3.9e-23 Amidase
Amidase PF01425 182 - 401 6.3e-18 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 431
AciI CCGC 2 cut(s) 431, 744
AclI AACGTT 1 cut(s) 110
AclWI GGATC 4 cut(s) 88, 112, 259, 689
AcsI RAATTY 1 cut(s) 1031
AcuI CTGAAG 1 cut(s) 759
AfiI CCNNNNNNNGG 4 cut(s) 578, 598, 743, 1239
AgsI TTSAA 5 cut(s) 137, 157, 949, 1175, 1358
AhlI ACTAGT 2 cut(s) 767, 1094
AjnI CCWGG 1 cut(s) 1246
Alw21I GWGCWC 1 cut(s) 1223
Alw26I GTCTC 4 cut(s) 239, 420, 613, 960
AlwI GGATC 4 cut(s) 88, 112, 259, 689
AlwNI CAGNNNCTG 1 cut(s) 760
Ama87I CYCGRG 1 cut(s) 423
AoxI GGCC 3 cut(s) 635, 708, 772
ApeKI GCWGC 1 cut(s) 181
ApoI RAATTY 1 cut(s) 1031
ArsI GACNNNNNNTTYG 2 cut(s) 707, 739
AspLEI GCGC 2 cut(s) 539, 857
AspS9I GGNCC 2 cut(s) 635, 999
AsuC2I CCSGG 1 cut(s) 598
AsuHPI GGTGA 3 cut(s) 932, 981, 1118
AvaI CYCGRG 1 cut(s) 423
AvaII GGWCC 1 cut(s) 999
BaeI ACNNNNGTAYC 2 cut(s) 1261, 1294
BanII GRGCYC 2 cut(s) 502, 1223
BauI CACGAG 1 cut(s) 586
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 1 cut(s) 1223
BbvI GCAGC 1 cut(s) 193
BccI CCATC 2 cut(s) 521, 527
BceAI ACGGC 1 cut(s) 402
BciT130I CCWGG 1 cut(s) 1248
BciVI GTATCC 2 cut(s) 1254, 1262
BclI TGATCA 1 cut(s) 823
BcnI CCSGG 1 cut(s) 598
BcoDI GTCTC 4 cut(s) 239, 420, 613, 960
BcuI ACTAGT 2 cut(s) 767, 1094
BfaI CTAG 3 cut(s) 768, 1095, 1229
BfmI CTRYAG 1 cut(s) 1263
BfoI RGCGCY 1 cut(s) 858
BfuI GTATCC 2 cut(s) 1254, 1262
BisI GCNGC 1 cut(s) 182
BlpI GCTNAGC 1 cut(s) 101
BlsI GCNGC 1 cut(s) 183
Bme1390I CCNGG 2 cut(s) 598, 1248
Bme18I GGWCC 1 cut(s) 999
BmeT110I CYCGRG 1 cut(s) 423
BmgT120I GGNCC 2 cut(s) 635, 999
BmrFI CCNGG 2 cut(s) 598, 1248
BmsI GCATC 4 cut(s) 247, 649, 667, 1180
BpiI GAAGAC 1 cut(s) 144
Bpu10I CCTNAGC 1 cut(s) 1003
Bpu1102I GCTNAGC 1 cut(s) 101
BpuEI CTTGAG 3 cut(s) 42, 332, 637
BpuMI CCSGG 1 cut(s) 598
BsaJI CCNNGG 3 cut(s) 366, 384, 597
BsaWI WCCGGW 2 cut(s) 337, 996
Bsc4I CCNNNNNNNGG 4 cut(s) 578, 598, 743, 1239
Bse1I ACTGG 2 cut(s) 483, 992
BseBI CCWGG 1 cut(s) 1248
BseDI CCNNGG 3 cut(s) 366, 384, 597
BseGI GGATG 2 cut(s) 519, 664
BseLI CCNNNNNNNGG 4 cut(s) 578, 598, 743, 1239
BseMII CTCAG 5 cut(s) 104, 440, 933, 1304, 1335
BseNI ACTGG 2 cut(s) 483, 992
BseRI GAGGAG 1 cut(s) 1296
BseXI GCAGC 1 cut(s) 193
Bsh1236I CGCG 1 cut(s) 431
Bsh1285I CGRYCG 1 cut(s) 417
BshFI GGCC 3 cut(s) 637, 710, 774
BsiEI CGRYCG 1 cut(s) 417
BsiHKAI GWGCWC 1 cut(s) 1223
BsiHKCI CYCGRG 1 cut(s) 423
BsiSI CCGG 3 cut(s) 338, 597, 997
BslFI GGGAC 3 cut(s) 922, 1077, 1244
BslI CCNNNNNNNGG 4 cut(s) 578, 598, 743, 1239
BsmAI GTCTC 4 cut(s) 239, 420, 613, 960
BsmBI CGTCTC 2 cut(s) 239, 420
BsmFI GGGAC 3 cut(s) 922, 1077, 1244
BsmI GAATGC 1 cut(s) 808
BsnI GGCC 3 cut(s) 637, 710, 774
BsoBI CYCGRG 1 cut(s) 423
Bsp1286I GDGCHC 2 cut(s) 502, 1223
Bsp143I GATC 9 cut(s) 80, 93, 117, 264, 276, 468, 509, 694, 823
Bsp1720I GCTNAGC 1 cut(s) 101
BspACI CCGC 2 cut(s) 431, 744
BspANI GGCC 3 cut(s) 637, 710, 774
BspCNI CTCAG 5 cut(s) 103, 441, 934, 1305, 1334
BspFNI CGCG 1 cut(s) 431
BspMAI CTGCAG 1 cut(s) 1267
BspPI GGATC 4 cut(s) 88, 112, 259, 689
BsrI ACTGG 2 cut(s) 483, 992
BssECI CCNNGG 3 cut(s) 366, 384, 597
BssMI GATC 9 cut(s) 80, 93, 117, 264, 276, 468, 509, 694, 823
BssSI CACGAG 1 cut(s) 586
BssT1I CCWWGG 1 cut(s) 366
Bst2BI CACGAG 1 cut(s) 586
Bst2UI CCWGG 1 cut(s) 1248
Bst4CI ACNGT 3 cut(s) 332, 629, 652
Bst6I CTCTTC 4 cut(s) 39, 822, 918, 1170
BstC8I GCNNGC 1 cut(s) 391
BstDEI CTNAG 8 cut(s) 90, 101, 312, 449, 942, 1003, 1313, 1321
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 2 cut(s) 519, 664
BstFNI CGCG 1 cut(s) 431
BstH2I RGCGCY 1 cut(s) 858
BstHHI GCGC 2 cut(s) 539, 857
BstKTI GATC 9 cut(s) 83, 96, 120, 267, 279, 471, 512, 697, 826
BstMAI GTCTC 4 cut(s) 239, 420, 613, 960
BstMBI GATC 9 cut(s) 80, 93, 117, 264, 276, 468, 509, 694, 823
BstMCI CGRYCG 1 cut(s) 417
BstMWI GCNNNNNNNGC 3 cut(s) 278, 428, 938
BstNI CCWGG 1 cut(s) 1248
BstSCI CCNGG 2 cut(s) 596, 1246
BstSFI CTRYAG 1 cut(s) 1263
BstUI CGCG 1 cut(s) 431
BstV1I GCAGC 1 cut(s) 193
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 1 cut(s) 80
BstXI CCANNNNNNTGG 1 cut(s) 531
BstYI RGATCY 1 cut(s) 80
BsuI GTATCC 2 cut(s) 1254, 1262
BsuRI GGCC 3 cut(s) 637, 710, 774
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 2 cut(s) 519, 664
BtsIMutI CAGTG 1 cut(s) 657
Cac8I GCNNGC 1 cut(s) 391
CaiI CAGNNNCTG 1 cut(s) 760
CfoI GCGC 2 cut(s) 539, 857
Cfr13I GGNCC 2 cut(s) 635, 999
CseI GACGC 2 cut(s) 437, 711
DdeI CTNAG 8 cut(s) 90, 101, 312, 449, 942, 1003, 1313, 1321
DpnI GATC 9 cut(s) 82, 95, 119, 266, 278, 470, 511, 696, 825
DpnII GATC 9 cut(s) 80, 93, 117, 264, 276, 468, 509, 694, 823
Eam1104I CTCTTC 4 cut(s) 39, 822, 918, 1170
EarI CTCTTC 4 cut(s) 39, 822, 918, 1170
Ecl136II GAGCTC 1 cut(s) 1221
Eco130I CCWWGG 1 cut(s) 366
Eco24I GRGCYC 2 cut(s) 502, 1223
Eco47I GGWCC 1 cut(s) 999
Eco53kI GAGCTC 1 cut(s) 1221
Eco57I CTGAAG 1 cut(s) 759
Eco88I CYCGRG 1 cut(s) 423
EcoICRI GAGCTC 1 cut(s) 1221
EcoRI GAATTC 1 cut(s) 1031
EcoRII CCWGG 1 cut(s) 1246
EcoT14I CCWWGG 1 cut(s) 366
EcoT22I ATGCAT 1 cut(s) 967
EcoT38I GRGCYC 2 cut(s) 502, 1223
ErhI CCWWGG 1 cut(s) 366
Esp3I CGTCTC 2 cut(s) 239, 420
FaiI YATR 8 cut(s) 71, 359, 693, 734, 967, 1291, 1293, 1394
FalI AAGNNNNNCTT 2 cut(s) 1353, 1385
FaqI GGGAC 3 cut(s) 922, 1077, 1244
FauI CCCGC 1 cut(s) 424
FauNDI CATATG 1 cut(s) 1291
FbaI TGATCA 1 cut(s) 823
Fnu4HI GCNGC 1 cut(s) 182
FokI GGATG 2 cut(s) 506, 671
FriOI GRGCYC 2 cut(s) 502, 1223
Fsp4HI GCNGC 1 cut(s) 182
FspBI CTAG 3 cut(s) 768, 1095, 1229
GlaI GCGC 2 cut(s) 538, 856
GluI GCNGC 1 cut(s) 182
HaeII RGCGCY 1 cut(s) 858
HaeIII GGCC 3 cut(s) 637, 710, 774
HapII CCGG 3 cut(s) 338, 597, 997
HgaI GACGC 2 cut(s) 437, 711
HhaI GCGC 2 cut(s) 539, 857
Hin6I GCGC 2 cut(s) 537, 855
HinP1I GCGC 2 cut(s) 537, 855
HindIII AAGCTT 2 cut(s) 314, 374
HinfI GANTC 7 cut(s) 250, 353, 455, 582, 1158, 1251, 1317
HpaII CCGG 3 cut(s) 338, 597, 997
HphI GGTGA 3 cut(s) 932, 981, 1118
Hpy188I TCNGA 9 cut(s) 93, 549, 581, 658, 727, 762, 876, 1213, 1324
Hpy188III TCNNGA 9 cut(s) 115, 200, 244, 254, 425, 562, 586, 821, 949
HpyAV CCTTC 2 cut(s) 734, 845
HpyCH4III ACNGT 3 cut(s) 332, 629, 652
HpyCH4IV ACGT 2 cut(s) 110, 292
HpyCH4V TGCA 5 cut(s) 328, 965, 1129, 1193, 1265
HpyF10VI GCNNNNNNNGC 3 cut(s) 278, 428, 938
HpyF3I CTNAG 8 cut(s) 90, 101, 312, 449, 942, 1003, 1313, 1321
HpySE526I ACGT 2 cut(s) 110, 292
HspAI GCGC 2 cut(s) 537, 855
Ksp22I TGATCA 1 cut(s) 823
Kzo9I GATC 9 cut(s) 80, 93, 117, 264, 276, 468, 509, 694, 823
LmnI GCTCC 1 cut(s) 534
Lsp1109I GCAGC 1 cut(s) 193
LweI GCATC 4 cut(s) 247, 649, 667, 1180
MaeI CTAG 3 cut(s) 768, 1095, 1229
MaeII ACGT 2 cut(s) 110, 292
MaeIII GTNAC 3 cut(s) 293, 746, 1198
MalI GATC 9 cut(s) 82, 95, 119, 266, 278, 470, 511, 696, 825
MboI GATC 9 cut(s) 80, 93, 117, 264, 276, 468, 509, 694, 823
MflI RGATCY 1 cut(s) 80
MhlI GDGCHC 2 cut(s) 502, 1223
MluCI AATT 2 cut(s) 1031, 1170
MlyI GAGTC 1 cut(s) 576
MmeI TCCRAC 4 cut(s) 58, 512, 559, 636
MnlI CCTC 9 cut(s) 97, 700, 785, 1021, 1063, 1143, 1232, 1274, 1301
Mph1103I ATGCAT 1 cut(s) 967
MseI TTAA 9 cut(s) 219, 300, 378, 441, 488, 567, 842, 867, 972
MspI CCGG 3 cut(s) 338, 597, 997
MspR9I CCNGG 2 cut(s) 598, 1248
Mva1269I GAATGC 1 cut(s) 808
MvaI CCWGG 1 cut(s) 1248
MvnI CGCG 1 cut(s) 431
MwoI GCNNNNNNNGC 3 cut(s) 278, 428, 938
NciI CCSGG 1 cut(s) 598
NdeI CATATG 1 cut(s) 1291
NdeII GATC 9 cut(s) 80, 93, 117, 264, 276, 468, 509, 694, 823
NmeAIII GCCGAG 1 cut(s) 617
NmuCI GTSAC 2 cut(s) 293, 1198
NsiI ATGCAT 1 cut(s) 967
PaeR7I CTCGAG 1 cut(s) 423
PcsI WCGNNNNNNNCGW 3 cut(s) 240, 392, 719
PctI GAATGC 1 cut(s) 808
PfeI GAWTC 6 cut(s) 250, 353, 455, 1158, 1251, 1317
PflFI GACNNNGTC 1 cut(s) 652
PfoI TCCNGGA 1 cut(s) 1246
PkrI GCNGC 1 cut(s) 183
PleI GAGTC 1 cut(s) 576
PpsI GAGTC 1 cut(s) 576
Psp124BI GAGCTC 1 cut(s) 1223
Psp1406I AACGTT 1 cut(s) 110
Psp6I CCWGG 1 cut(s) 1246
PspGI CCWGG 1 cut(s) 1246
PspPI GGNCC 2 cut(s) 635, 999
PstI CTGCAG 1 cut(s) 1267
PstNI CAGNNNCTG 1 cut(s) 760
PsuI RGATCY 1 cut(s) 80
PsyI GACNNNGTC 1 cut(s) 652
SacI GAGCTC 1 cut(s) 1223
SaqAI TTAA 9 cut(s) 219, 300, 378, 441, 488, 567, 842, 867, 972
SatI GCNGC 1 cut(s) 182
Sau3AI GATC 9 cut(s) 80, 93, 117, 264, 276, 468, 509, 694, 823
Sau96I GGNCC 2 cut(s) 635, 999
SbfI CCTGCAGG 1 cut(s) 1267
SchI GAGTC 1 cut(s) 576
ScrFI CCNGG 2 cut(s) 598, 1248
SdaI CCTGCAGG 1 cut(s) 1267
SduI GDGCHC 2 cut(s) 502, 1223
SfaNI GCATC 4 cut(s) 247, 649, 667, 1180
SfcI CTRYAG 1 cut(s) 1263
Sfr274I CTCGAG 1 cut(s) 423
SinI GGWCC 1 cut(s) 999
SlaI CTCGAG 1 cut(s) 423
SmlI CTYRAG 4 cut(s) 57, 347, 423, 616
SmoI CTYRAG 4 cut(s) 57, 347, 423, 616
SpeI ACTAGT 2 cut(s) 767, 1094
Sse8387I CCTGCAGG 1 cut(s) 1267
Sse9I AATT 2 cut(s) 1031, 1170
SsiI CCGC 2 cut(s) 431, 744
SspMI CTAG 3 cut(s) 768, 1095, 1229
SstI GAGCTC 1 cut(s) 1223
StyD4I CCNGG 2 cut(s) 596, 1246
StyI CCWWGG 1 cut(s) 366
TaaI ACNGT 3 cut(s) 332, 629, 652
TaiI ACGT 2 cut(s) 113, 295
TaqI TCGA 8 cut(s) 116, 201, 243, 424, 508, 891, 1148, 1161
TaqII GACCGA 1 cut(s) 403
TasI AATT 2 cut(s) 1031, 1170
TfiI GAWTC 6 cut(s) 250, 353, 455, 1158, 1251, 1317
Tru1I TTAA 9 cut(s) 219, 300, 378, 441, 488, 567, 842, 867, 972
Tru9I TTAA 9 cut(s) 219, 300, 378, 441, 488, 567, 842, 867, 972
TscAI CASTG 1 cut(s) 657
TseFI GTSAC 2 cut(s) 293, 1198
TseI GCWGC 1 cut(s) 181
Tsp45I GTSAC 2 cut(s) 293, 1198
TspDTI ATGAA 5 cut(s) 656, 721, 818, 1151, 1381
TspRI CASTG 1 cut(s) 657
Tth111I GACNNNGTC 1 cut(s) 652
VpaK11BI GGWCC 1 cut(s) 999
XapI RAATTY 1 cut(s) 1031
XcmI CCANNNNNNNNNTGG 1 cut(s) 1099
XhoI CTCGAG 1 cut(s) 423
XspI CTAG 3 cut(s) 768, 1095, 1229
Zsp2I ATGCAT 1 cut(s) 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.