Rw0G014670

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00654
OG0000427
Physical Location & Seq
Reverse (-)
3520 .. 19405
15886 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G014670.1

Sequence Viewer

Length: 3045 bp
ATGATCATTTCCTCATCGACTGCCATGGCAAAACCAAGCTTCCCTCACATCTTATCCTTATTGCTTCTCATTCTTCTCTTCACATTTTCATCTCTGCCAGGCACATTCGCAGCTCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTTAAGCACAACCAACTCACCTCGAGGCAGCTTGTCCAATATTACCATTCCCAAATCCGAAAACTGAACCCGGTTCTCAAAGGAGTCCTAGAAGTAAACCCGGATGCGCTTCACCTTGCCGACAAGGCTGACTACGAGCGCAGGACCAAGGCACCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCAACCAAGGATAAGATGAACACCACCGCCGGCTCTTTTGCACTGCTTGGATCTGTCGTGCCTCGTGATGCTGGCGTGGTGGAGAAGTTGAGGAAAGCTGGGGCTATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCACATTTTAGGTCTAATAAAGCACCCTCCGGTTGGAACGCCAGAGGCGGTTATGGCTTGAACCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTGGCAGCAAATCTGGCAGCGGTTTCATTAGGTACTGAGACAGATGGTTCAATCTTATGTCCATCAGGTTATAACTCAGTTGTGGGCATCAAACCAACAATCGGTCTTACTAGTCGAGCAGGGGTTGTCCCAATTTCTCTGAGGCAGGATACTGTCGGGCCAATTTGTAGGACAGTAGCCGATGCTGCTTATGTTCTTGATGCCATAGCAGGCATCGACCACAATGACATTGCAACAATTGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGACTTGGAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATAACTTTGGCAATGACACTGCCTTGGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAAAGGAGGTGCTGTTTTGGTAGACAATTTGGAAATTGCTAATTTGACTGAAATCTATTCTTCAAGCGATGAAGATACTGCATTGTCTGTTGAGTGCAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCAGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACCTATTTTTAGAAGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCGGGCATTGTTGAATCTTGCAAGGTGGACAAGAATTGGTCTTGTGAAATTGGTGACAGAGAAGAAGCTAGATGCTGTAGTGACTCCTAAAGCAGATTTTTCAAAGATACTTGCAATTGGGGGAGCCCCAGGTCTAATAGTTCCAGCCGGATACGAGAACAATGGGAGACCAGCTGAAATGATAATTTCCTCATCGACTGCCATGACGAAACCAAGCTTCCCTCACATCTTATCTTTATTGCATCTCATTCTTCTCTTCACATTATCATCTCTGTCAGGCACATTCGCAGCCCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTTAAGCACAACCAACTCACCTCGAGGCAACTTGTGCAATATTACCTTTCCCAAATCCGAAAACTTAACCCGGTTCTCAAAGGAGTCCTAGAAGTCAACCCGGATGCGCTTCACCTTGCCGACAAGGCTGACGATGAGCGCAGGACCAAGGCATCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCAACCAAGGATAAGATGAACACTACCGCCGGCTCTTTTGCGCTTCTTGGATCTGTCGTCCCTCGTGATGCCGGAGTGGTGGAGAAGTTGAGGAAAGAAGGGGCCATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCAAATTCTAGGTCTAATAATACAGCACCAAATGGTTGGAGCGCCAGAGGCGGTTATGGCTTGAACCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTAGCAGCAAATCTGGCAGCGGTGTCATTAGGTACCGAGACAGATGGTTCAATCTTATGTCCATCAGGTTATAACTCGGTTGTGGGCATCAAACCAACAATCGGTCTTACTAGTCGAGCAGGAGTTGTCCCAATTTCTCCAAGACAAGATACTGTTGGGCCAATTTGTAGGACAGTAGCAGATGCTACTTATGTTCTTGATGCCATAGCAGGAATCGACCACAATGACATTGCAACGATTGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCGACGGACTTAGAGGAAAAAGAATAGGGATACTGAGAGGCTTCTATGACTTTGGCAATGACACGTCCTTGGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAAAGGAGGTGCTGTTTTGGTAGACAATTTGGAAATTGCTAATTTGACTGAAATCTATTCTTCAAGCGATGAAGATACTGCATTGTTTGTTGAGTACAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCCGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACGTATTTTTAGCTGTTGAAGCAACAAATGGATTTGGAAAAACAGAGAAGCGAGCATTGTTGAATCTTGCAAGGTGGACAAGAAATGGTCTTGTAAAATTGGTGACAGAGAGGAAGCTAGATGCTGTGGTGACTCCTAAATCAGATTTTCGAACTGTACTTGCAATTGGGGGAGCCCCAGGTCTAACAGTTCCAGCTGGATACGAAAACAATGGGAGACCAGCTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATTGAGATTGCATATGCTTTTGAGCAAGCCACTAAGATTAGGAAGCCTCCTTCGTTCAAGGGCTTCAACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

1014

Amino Acids

109.09

Weight (kDa)

9.39

Isoelectric Point (pI)

34.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 65 - 401 6.6e-67 Amidase
Amidase PF01425 553 - 947 1.1e-67 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 696, 2163
AbsI CCTCGAGG 2 cut(s) 184, 1648
Acc65I GGTACC 1 cut(s) 2123
AccB1I GGYRCC 2 cut(s) 313, 2123
AccB7I CCANNNNNTGG 1 cut(s) 1210
AccI GTMKAC 2 cut(s) 1041, 2508
AccII CGCG 2 cut(s) 915, 2382
AclWI GGATC 4 cut(s) 430, 1161, 1894, 2628
AcsI RAATTY 1 cut(s) 1978
AcuI CTGAAG 1 cut(s) 1275
AfaI GTAC 6 cut(s) 658, 878, 2125, 2345, 2582, 2862
AfiI CCNNNNNNNGG 7 cut(s) 543, 725, 890, 1210, 2192, 2357, 2677
AflIII ACRYGT 1 cut(s) 2442
AhlI ACTAGT 2 cut(s) 734, 2201
AjiI CACGTC 1 cut(s) 2445
AjnI CCWGG 3 cut(s) 97, 1414, 2881
Alw26I GTCTC 6 cut(s) 656, 860, 1447, 2123, 2327, 2914
AlwI GGATC 4 cut(s) 430, 1161, 1894, 2628
AlwNI CAGNNNCTG 1 cut(s) 1210
Ama87I CYCGRG 2 cut(s) 184, 1648
AoxI GGCC 3 cut(s) 782, 1938, 2249
ApoI RAATTY 1 cut(s) 1978
ArsI GACNNNNNNTTYG 2 cut(s) 1970, 2002
Asp718I GGTACC 1 cut(s) 2123
AspLEI GCGC 8 cut(s) 271, 303, 915, 1735, 1767, 1879, 2018, 2382
AspS9I GGNCC 5 cut(s) 306, 782, 1770, 1938, 2249
AsuC2I CCSGG 4 cut(s) 233, 263, 1697, 1727
AsuHPI GGTGA 7 cut(s) 172, 266, 1351, 1636, 1730, 2818, 2845
AsuII TTCGAA 1 cut(s) 2854
AsuNHI GCTAGC 2 cut(s) 494, 1958
AvaI CYCGRG 2 cut(s) 184, 1648
AvaII GGWCC 2 cut(s) 306, 1770
BanI GGYRCC 2 cut(s) 313, 2123
BanII GRGCYC 2 cut(s) 1414, 2881
BauI CACGAG 2 cut(s) 435, 1899
BccI CCATC 5 cut(s) 662, 694, 1949, 2129, 2161
BciT130I CCWGG 3 cut(s) 99, 1416, 2883
BciVI GTATCC 4 cut(s) 766, 1430, 2403, 2897
BclI TGATCA 1 cut(s) 3
BcnI CCSGG 4 cut(s) 233, 263, 1697, 1727
BcoDI GTCTC 6 cut(s) 656, 860, 1447, 2123, 2327, 2914
BcuI ACTAGT 2 cut(s) 734, 2201
BfaI CTAG 9 cut(s) 251, 495, 735, 1355, 1715, 1959, 1983, 2202, 2822
BfmI CTRYAG 1 cut(s) 1362
BfoI RGCGCY 1 cut(s) 2019
BfuI GTATCC 4 cut(s) 766, 1430, 2403, 2897
BglI GCCNNNNNGGC 2 cut(s) 287, 1751
Bme1390I CCNGG 7 cut(s) 99, 233, 263, 1416, 1697, 1727, 2883
Bme18I GGWCC 2 cut(s) 306, 1770
BmeT110I CYCGRG 2 cut(s) 184, 1648
BmgBI CACGTC 1 cut(s) 2445
BmgT120I GGNCC 5 cut(s) 306, 782, 1770, 1938, 2249
BmiI GGNNCC 5 cut(s) 315, 1411, 1939, 2125, 2878
BmrFI CCNGG 7 cut(s) 99, 233, 263, 1416, 1697, 1727, 2883
BmrI ACTGGG 2 cut(s) 1154, 2621
BmtI GCTAGC 2 cut(s) 498, 1962
BmuI ACTGGG 2 cut(s) 1154, 2621
BpmI CTGGAG 2 cut(s) 137, 1601
Bpu14I TTCGAA 1 cut(s) 2854
BpuEI CTTGAG 4 cut(s) 520, 586, 1984, 2053
BpuMI CCSGG 4 cut(s) 233, 263, 1697, 1727
BsaI GGTCTC 2 cut(s) 1447, 2914
BsaWI WCCGGW 1 cut(s) 539
BsaXI ACNNNNNCTCC 4 cut(s) 1017, 1047, 2484, 2514
Bsc4I CCNNNNNNNGG 7 cut(s) 543, 725, 890, 1210, 2192, 2357, 2677
Bse118I RCCGGY 2 cut(s) 401, 1865
Bse1I ACTGG 5 cut(s) 317, 1160, 1215, 2627, 2682
Bse3DI GCAATG 6 cut(s) 369, 852, 976, 1833, 2319, 2443
BseBI CCWGG 3 cut(s) 99, 1416, 2883
BseGI GGATG 2 cut(s) 271, 1735
BseLI CCNNNNNNNGG 7 cut(s) 543, 725, 890, 1210, 2192, 2357, 2677
BseMI GCAATG 6 cut(s) 369, 852, 976, 1833, 2319, 2443
BseNI ACTGG 5 cut(s) 317, 1160, 1215, 2627, 2682
BseYI CCCAGC 1 cut(s) 470
Bsh1236I CGCG 2 cut(s) 915, 2382
BshFI GGCC 3 cut(s) 784, 1940, 2251
BshNI GGYRCC 2 cut(s) 313, 2123
BsiHKCI CYCGRG 2 cut(s) 184, 1648
BsiSI CCGG 9 cut(s) 233, 263, 402, 540, 1434, 1697, 1727, 1866, 1908
BslFI GGGAC 3 cut(s) 737, 1880, 2204
BslI CCNNNNNNNGG 7 cut(s) 543, 725, 890, 1210, 2192, 2357, 2677
BsmAI GTCTC 6 cut(s) 656, 860, 1447, 2123, 2327, 2914
BsmFI GGGAC 3 cut(s) 737, 1880, 2204
BsmI GAATGC 2 cut(s) 345, 1809
BsnI GGCC 3 cut(s) 784, 1940, 2251
Bso31I GGTCTC 2 cut(s) 1447, 2914
BsoBI CYCGRG 2 cut(s) 184, 1648
Bsp119I TTCGAA 1 cut(s) 2854
Bsp1286I GDGCHC 2 cut(s) 1414, 2881
Bsp143I GATC 9 cut(s) 3, 148, 422, 1166, 1218, 1612, 1886, 2633, 2685
Bsp19I CCATGG 1 cut(s) 24
BspANI GGCC 3 cut(s) 784, 1940, 2251
BspFNI CGCG 2 cut(s) 915, 2382
BspLI GGNNCC 5 cut(s) 315, 1411, 1939, 2125, 2878
BspOI GCTAGC 2 cut(s) 498, 1962
BspPI GGATC 4 cut(s) 430, 1161, 1894, 2628
BspT104I TTCGAA 1 cut(s) 2854
BspT107I GGYRCC 2 cut(s) 313, 2123
BspTNI GGTCTC 2 cut(s) 1447, 2914
BsrDI GCAATG 6 cut(s) 369, 852, 976, 1833, 2319, 2443
BsrFI RCCGGY 2 cut(s) 401, 1865
BsrI ACTGG 5 cut(s) 317, 1160, 1215, 2627, 2682
BssAI RCCGGY 2 cut(s) 401, 1865
BssMI GATC 9 cut(s) 3, 148, 422, 1166, 1218, 1612, 1886, 2633, 2685
BssSI CACGAG 2 cut(s) 435, 1899
BssT1I CCWWGG 9 cut(s) 24, 309, 378, 981, 1170, 1773, 1842, 2448, 2637
Bst2BI CACGAG 2 cut(s) 435, 1899
Bst2UI CCWGG 3 cut(s) 99, 1416, 2883
Bst4CI ACNGT 8 cut(s) 778, 799, 903, 2245, 2266, 2370, 2860, 2893
Bst6I CTCTTC 2 cut(s) 83, 1547
BstAPI GCANNNNNTGC 1 cut(s) 1660
BstBI TTCGAA 1 cut(s) 2854
BstDSI CCRYGG 1 cut(s) 24
BstF5I GGATG 2 cut(s) 271, 1735
BstFNI CGCG 2 cut(s) 915, 2382
BstH2I RGCGCY 1 cut(s) 2019
BstHHI GCGC 8 cut(s) 271, 303, 915, 1735, 1767, 1879, 2018, 2382
BstKTI GATC 9 cut(s) 6, 151, 425, 1169, 1221, 1615, 1889, 2636, 2688
BstMAI GTCTC 6 cut(s) 656, 860, 1447, 2123, 2327, 2914
BstMBI GATC 9 cut(s) 3, 148, 422, 1166, 1218, 1612, 1886, 2633, 2685
BstNI CCWGG 3 cut(s) 99, 1416, 2883
BstSCI CCNGG 7 cut(s) 97, 231, 261, 1414, 1695, 1725, 2881
BstSFI CTRYAG 1 cut(s) 1362
BstUI CGCG 2 cut(s) 915, 2382
BstX2I RGATCY 2 cut(s) 422, 1886
BstXI CCANNNNNNTGG 2 cut(s) 1948, 2010
BstYI RGATCY 2 cut(s) 422, 1886
BsuI GTATCC 4 cut(s) 766, 1430, 2403, 2897
BsuRI GGCC 3 cut(s) 784, 1940, 2251
BtgI CCRYGG 1 cut(s) 24
BtgZI GCGATG 2 cut(s) 1101, 2568
BtrI CACGTC 1 cut(s) 2445
BtsCI GGATG 2 cut(s) 271, 1735
BtsI GCAGTG 2 cut(s) 413, 975
BtsIMutI CAGTG 7 cut(s) 324, 413, 630, 975, 1167, 1788, 2634
CaiI CAGNNNCTG 1 cut(s) 1210
CfoI GCGC 8 cut(s) 271, 303, 915, 1735, 1767, 1879, 2018, 2382
Cfr10I RCCGGY 2 cut(s) 401, 1865
Cfr13I GGNCC 5 cut(s) 306, 782, 1770, 1938, 2249
Csp6I GTAC 6 cut(s) 657, 877, 2124, 2344, 2581, 2861
CspCI CAANNNNNGTGG 2 cut(s) 606, 641
CviAII CATG 4 cut(s) 25, 341, 1489, 1805
CviQI GTAC 6 cut(s) 657, 877, 2124, 2344, 2581, 2861
DpnI GATC 9 cut(s) 5, 150, 424, 1168, 1220, 1614, 1888, 2635, 2687
DpnII GATC 9 cut(s) 3, 148, 422, 1166, 1218, 1612, 1886, 2633, 2685
Eam1104I CTCTTC 2 cut(s) 83, 1547
EarI CTCTTC 2 cut(s) 83, 1547
Eco130I CCWWGG 9 cut(s) 24, 309, 378, 981, 1170, 1773, 1842, 2448, 2637
Eco24I GRGCYC 2 cut(s) 1414, 2881
Eco31I GGTCTC 2 cut(s) 1447, 2914
Eco47I GGWCC 2 cut(s) 306, 1770
Eco57I CTGAAG 1 cut(s) 1275
Eco88I CYCGRG 2 cut(s) 184, 1648
EcoRII CCWGG 3 cut(s) 97, 1414, 2881
EcoT14I CCWWGG 9 cut(s) 24, 309, 378, 981, 1170, 1773, 1842, 2448, 2637
EcoT22I ATGCAT 2 cut(s) 1135, 2602
EcoT38I GRGCYC 2 cut(s) 1414, 2881
ErhI CCWWGG 9 cut(s) 24, 309, 378, 981, 1170, 1773, 1842, 2448, 2637
FaeI CATG 4 cut(s) 28, 344, 1492, 1808
FalI AAGNNNNNCTT 4 cut(s) 482, 514, 1946, 1978
FaqI GGGAC 3 cut(s) 737, 1880, 2204
FatI CATG 4 cut(s) 24, 340, 1488, 1804
FauI CCCGC 2 cut(s) 1281, 1586
FauNDI CATATG 1 cut(s) 2983
FbaI TGATCA 1 cut(s) 3
FblI GTMKAC 2 cut(s) 1041, 2508
FokI GGATG 2 cut(s) 278, 1742
FriOI GRGCYC 2 cut(s) 1414, 2881
FspBI CTAG 9 cut(s) 251, 495, 735, 1355, 1715, 1959, 1983, 2202, 2822
GlaI GCGC 8 cut(s) 270, 302, 914, 1734, 1766, 1878, 2017, 2381
GsaI CCCAGC 1 cut(s) 474
GsuI CTGGAG 2 cut(s) 137, 1601
HaeII RGCGCY 1 cut(s) 2019
HaeIII GGCC 3 cut(s) 784, 1940, 2251
HapII CCGG 9 cut(s) 233, 263, 402, 540, 1434, 1697, 1727, 1866, 1908
HhaI GCGC 8 cut(s) 271, 303, 915, 1735, 1767, 1879, 2018, 2382
Hin1II CATG 4 cut(s) 28, 344, 1492, 1808
Hin6I GCGC 8 cut(s) 269, 301, 913, 1733, 1765, 1877, 2016, 2380
HinP1I GCGC 8 cut(s) 269, 301, 913, 1733, 1765, 1877, 2016, 2380
HincII GTYRAC 1 cut(s) 1723
HindII GTYRAC 1 cut(s) 1723
HindIII AAGCTT 2 cut(s) 37, 1501
HinfI GANTC 9 cut(s) 120, 246, 1300, 1369, 1584, 1710, 2304, 2767, 2836
HpaII CCGG 9 cut(s) 233, 263, 402, 540, 1434, 1697, 1727, 1866, 1908
HphI GGTGA 7 cut(s) 172, 266, 1351, 1636, 1730, 2818, 2845
Hpy166II GTNNAC 7 cut(s) 259, 1042, 1314, 1723, 2509, 2781, 2945
Hpy188I TCNGA 9 cut(s) 221, 589, 765, 1017, 1685, 2056, 2484, 2848, 2959
Hpy188III TCNNGA 4 cut(s) 437, 821, 1901, 2288
Hpy8I GTNNAC 7 cut(s) 259, 1042, 1314, 1723, 2509, 2781, 2945
Hpy99I CGWCG 2 cut(s) 920, 2387
HpyAV CCTTC 7 cut(s) 964, 1135, 1198, 1928, 2602, 2665, 3030
HpyCH4III ACNGT 8 cut(s) 778, 799, 903, 2245, 2266, 2370, 2860, 2893
HpyCH4IV ACGT 2 cut(s) 2444, 2706
HpySE526I ACGT 2 cut(s) 2444, 2706
Hsp92II CATG 4 cut(s) 28, 344, 1492, 1808
HspAI GCGC 8 cut(s) 269, 301, 913, 1733, 1765, 1877, 2016, 2380
KpnI GGTACC 1 cut(s) 2127
KroI GCCGGC 2 cut(s) 401, 1865
KroNI GCCGGC 2 cut(s) 403, 1867
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 9 cut(s) 3, 148, 422, 1166, 1218, 1612, 1886, 2633, 2685
LmnI GCTCC 3 cut(s) 1409, 2013, 2876
MaeI CTAG 9 cut(s) 251, 495, 735, 1355, 1715, 1959, 1983, 2202, 2822
MaeII ACGT 2 cut(s) 2444, 2706
MaeIII GTNAC 7 cut(s) 321, 510, 1339, 1366, 1785, 2806, 2833
MalI GATC 9 cut(s) 5, 150, 424, 1168, 1220, 1614, 1888, 2635, 2687
MboI GATC 9 cut(s) 3, 148, 422, 1166, 1218, 1612, 1886, 2633, 2685
MboII GAAGA 9 cut(s) 65, 70, 1071, 1103, 1360, 1529, 1534, 2538, 2570
MfeI CAATTG 3 cut(s) 861, 1401, 2868
MflI RGATCY 2 cut(s) 422, 1886
MhlI GDGCHC 2 cut(s) 1414, 2881
MlyI GAGTC 6 cut(s) 114, 255, 1363, 1578, 1719, 2830
MmeI TCCRAC 2 cut(s) 524, 1991
Mph1103I ATGCAT 2 cut(s) 1135, 2602
MroNI GCCGGC 2 cut(s) 401, 1865
MseI TTAA 5 cut(s) 165, 1127, 1629, 1692, 2594
MspA1I CMGCKG 7 cut(s) 609, 644, 1460, 2076, 2111, 2900, 2927
MspI CCGG 9 cut(s) 233, 263, 402, 540, 1434, 1697, 1727, 1866, 1908
MspR9I CCNGG 7 cut(s) 99, 233, 263, 1416, 1697, 1727, 2883
MunI CAATTG 3 cut(s) 861, 1401, 2868
Mva1269I GAATGC 2 cut(s) 345, 1809
MvaI CCWGG 3 cut(s) 99, 1416, 2883
MvnI CGCG 2 cut(s) 915, 2382
NaeI GCCGGC 2 cut(s) 403, 1867
NciI CCSGG 4 cut(s) 233, 263, 1697, 1727
NcoI CCATGG 1 cut(s) 24
NdeI CATATG 1 cut(s) 2983
NdeII GATC 9 cut(s) 3, 148, 422, 1166, 1218, 1612, 1886, 2633, 2685
NgoMIV GCCGGC 2 cut(s) 401, 1865
NheI GCTAGC 2 cut(s) 494, 1958
NlaIII CATG 4 cut(s) 28, 344, 1492, 1808
NlaIV GGNNCC 5 cut(s) 315, 1411, 1939, 2125, 2878
NmuCI GTSAC 7 cut(s) 321, 510, 1339, 1366, 1785, 2806, 2833
NsiI ATGCAT 2 cut(s) 1135, 2602
NspV TTCGAA 1 cut(s) 2854
PaeR7I CTCGAG 2 cut(s) 184, 1648
PctI GAATGC 2 cut(s) 345, 1809
PdiI GCCGGC 2 cut(s) 403, 1867
PfeI GAWTC 3 cut(s) 1300, 2304, 2767
PflMI CCANNNNNTGG 1 cut(s) 1210
PleI GAGTC 6 cut(s) 114, 254, 1363, 1578, 1718, 2830
PpsI GAGTC 6 cut(s) 114, 254, 1363, 1578, 1718, 2830
PsiI TTATAA 2 cut(s) 696, 2163
Psp6I CCWGG 3 cut(s) 97, 1414, 2881
PspFI CCCAGC 1 cut(s) 470
PspGI CCWGG 3 cut(s) 97, 1414, 2881
PspN4I GGNNCC 5 cut(s) 315, 1411, 1939, 2125, 2878
PspPI GGNCC 5 cut(s) 306, 782, 1770, 1938, 2249
PspXI VCTCGAGB 2 cut(s) 184, 1648
PstNI CAGNNNCTG 1 cut(s) 1210
PsuI RGATCY 2 cut(s) 422, 1886
PvuII CAGCTG 3 cut(s) 1460, 2900, 2927
RsaI GTAC 6 cut(s) 658, 878, 2125, 2345, 2582, 2862
RsaNI GTAC 6 cut(s) 657, 877, 2124, 2344, 2581, 2861
SaqAI TTAA 5 cut(s) 165, 1127, 1629, 1692, 2594
Sau3AI GATC 9 cut(s) 3, 148, 422, 1166, 1218, 1612, 1886, 2633, 2685
Sau96I GGNCC 5 cut(s) 306, 782, 1770, 1938, 2249
SchI GAGTC 6 cut(s) 114, 255, 1363, 1578, 1719, 2830
ScrFI CCNGG 7 cut(s) 99, 233, 263, 1416, 1697, 1727, 2883
SduI GDGCHC 2 cut(s) 1414, 2881
SfcI CTRYAG 1 cut(s) 1362
Sfr274I CTCGAG 2 cut(s) 184, 1648
SfuI TTCGAA 1 cut(s) 2854
SinI GGWCC 2 cut(s) 306, 1770
SlaI CTCGAG 2 cut(s) 184, 1648
SmlI CTYRAG 6 cut(s) 184, 499, 601, 1648, 1963, 2068
SmoI CTYRAG 6 cut(s) 184, 499, 601, 1648, 1963, 2068
SpeI ACTAGT 2 cut(s) 734, 2201
SspI AATATT 2 cut(s) 203, 1667
SspMI CTAG 9 cut(s) 251, 495, 735, 1355, 1715, 1959, 1983, 2202, 2822
StyD4I CCNGG 7 cut(s) 97, 231, 261, 1414, 1695, 1725, 2881
StyI CCWWGG 9 cut(s) 24, 309, 378, 981, 1170, 1773, 1842, 2448, 2637
TaaI ACNGT 8 cut(s) 778, 799, 903, 2245, 2266, 2370, 2860, 2893
TaiI ACGT 2 cut(s) 2447, 2709
TaqI TCGA 9 cut(s) 17, 185, 739, 840, 1481, 1649, 2206, 2307, 2854
TaqII GACCGA 2 cut(s) 716, 2183
TatI WGTACW 4 cut(s) 876, 2343, 2580, 2860
TauI GCSGC 2 cut(s) 600, 2067
TfiI GAWTC 3 cut(s) 1300, 2304, 2767
Tru1I TTAA 5 cut(s) 165, 1127, 1629, 1692, 2594
Tru9I TTAA 5 cut(s) 165, 1127, 1629, 1692, 2594
TscAI CASTG 7 cut(s) 324, 420, 630, 982, 1167, 1788, 2634
TseFI GTSAC 7 cut(s) 321, 510, 1339, 1366, 1785, 2806, 2833
Tsp45I GTSAC 7 cut(s) 321, 510, 1339, 1366, 1785, 2806, 2833
TspDTI ATGAA 6 cut(s) 78, 404, 639, 1104, 1868, 2571
TspGWI ACGGA 2 cut(s) 933, 2400
TspRI CASTG 7 cut(s) 324, 420, 630, 982, 1167, 1788, 2634
Van91I CCANNNNNTGG 1 cut(s) 1210
VpaK11BI GGWCC 2 cut(s) 306, 1770
XapI RAATTY 1 cut(s) 1978
XhoI CTCGAG 2 cut(s) 184, 1648
XmiI GTMKAC 2 cut(s) 1041, 2508
XspI CTAG 9 cut(s) 251, 495, 735, 1355, 1715, 1959, 1983, 2202, 2822
Zsp2I ATGCAT 2 cut(s) 1135, 2602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.