Rh6BG020900

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000427
Physical Location & Seq
Reverse (-)
3090871 .. 3092329
1459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG020900.1

Sequence Viewer

Length: 1026 bp
ATGATGTGGATGCAGAATCCTTACAATTCATCGGTGGAAGTTTGTGGATCAAGTAGTGGACCTGCAATATCAGTAGCTGCTAATATGGTTTCTGTGACATTAGGGACGGAGACTGACAGCTCCATTATCTGTCCGGCTAGCTTTAACTCTGTGGTGGGCATCAAACCCACTGTTGGTCTCACTAGTCGAGCAGGAGTCATACCAATCAGTCCAAGACAGGACACAGTTGGGCCGATTTGTAGGACTGTAGCGGATGCTGTTCACGTCCTTGATATCATTGTTGGCATTGATAGGAATGATAATGCAACAATTGAAGCATCAAAGTACATCCCAGTGGGTGTCTATGGACAGTTTCTCAAGCATAATGGCCTCAAAGGAAAGAGACTGGGGATAGTAAATATGTTATTTGATTCAATCAAGGACGATGCTTTTCTGAAACAAACTTTTGTGCAGCATTTTACCACACTGAGGAAACAAGGTGCAATTTTGGTTGACAATCTTGAAATAGCCAATATTGAAGATGTTCTGGATTCTCATTCAAGTGGAGAATTTGATGCTATATTAGCTGTATTCAAAATAGCCTTGAATGAGTACCTAAGTGACCTAGTAAAATCTCCAGTGAAGTCCTTAAGGGAGGTGATAGAGTTCAACAAGAATCATTCAAAGGTGGAAAATATCAATGAATATGGGCAAGATGTCTTCGAATTAGCTGAGAAGACTAATGGGATGGGACCAAAAGAGCAGGAGGCATTGTCAAATTTAGAAAGACTATCTAGACAGGGGTTCAAGAAGTTGATGACCGATCATAGCCTAGATGCCTTGGTGGCTTATGCTAACAGTGCTTCTAGTATCCTCGCAATTGGTGGTTTTCCGGGCATTGTTGTGCCGGCAGGATATCATAACACTAAAAAGTATCCTTTTGGGATCTGCTTTGGGGGACTCAAGGGTTCAGAGCCAAAGCTGATTGAGATCGCCTATGCCTTTGAGCAAGCAACTAAGATGAGGAGAAAGCCTCCTCTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

341

Amino Acids

36.98

Weight (kDa)

6.4

Isoelectric Point (pI)

39.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 5 - 276 8.2e-36 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 70
AciI CCGC 1 cut(s) 251
AclWI GGATC 2 cut(s) 55, 932
AcsI RAATTY 2 cut(s) 548, 757
AfaI GTAC 2 cut(s) 326, 593
AfiI CCNNNNNNNGG 2 cut(s) 173, 468
AflII CTTAAG 1 cut(s) 628
AhlI ACTAGT 1 cut(s) 182
AjiI CACGTC 1 cut(s) 265
AluBI AGCT 6 cut(s) 77, 120, 141, 566, 710, 961
AluI AGCT 6 cut(s) 77, 120, 141, 566, 710, 961
Alw26I GTCTC 3 cut(s) 104, 182, 376
AlwI GGATC 2 cut(s) 55, 932
AlwNI CAGNNNCTG 1 cut(s) 77
AoxI GGCC 2 cut(s) 230, 367
ApeKI GCWGC 2 cut(s) 77, 451
ApoI RAATTY 2 cut(s) 548, 757
Asp700I GAANNNNTTC 1 cut(s) 522
AspS9I GGNCC 3 cut(s) 59, 230, 731
AsuC2I CCSGG 1 cut(s) 873
AsuHPI GGTGA 1 cut(s) 649
AsuII TTCGAA 1 cut(s) 702
AsuNHI GCTAGC 1 cut(s) 137
AvaII GGWCC 2 cut(s) 59, 731
BbsI GAAGAC 2 cut(s) 691, 722
BbvI GCAGC 2 cut(s) 64, 463
BccI CCATC 1 cut(s) 721
BciVI GTATCC 2 cut(s) 860, 924
BcnI CCSGG 1 cut(s) 873
BcoDI GTCTC 3 cut(s) 104, 182, 376
BcuI ACTAGT 1 cut(s) 182
BfaI CTAG 7 cut(s) 138, 183, 605, 774, 812, 846, 1019
BfmI CTRYAG 1 cut(s) 246
BfrI CTTAAG 1 cut(s) 628
BfuAI ACCTGC 1 cut(s) 70
BfuI GTATCC 2 cut(s) 860, 924
BglI GCCNNNNNGGC 1 cut(s) 824
BisI GCNGC 2 cut(s) 78, 452
BlsI GCNGC 2 cut(s) 79, 453
Bme1390I CCNGG 1 cut(s) 873
Bme18I GGWCC 2 cut(s) 59, 731
BmgBI CACGTC 1 cut(s) 265
BmgT120I GGNCC 3 cut(s) 59, 230, 731
BmiI GGNNCC 1 cut(s) 732
BmrFI CCNGG 1 cut(s) 873
BmrI ACTGGG 2 cut(s) 326, 395
BmsI GCATC 6 cut(s) 168, 244, 326, 415, 544, 805
BmtI GCTAGC 1 cut(s) 141
BmuI ACTGGG 2 cut(s) 326, 395
BpiI GAAGAC 2 cut(s) 691, 722
BplI GAGNNNNNCTC 1 cut(s) 997
BpmI CTGGAG 1 cut(s) 600
Bpu14I TTCGAA 1 cut(s) 702
BpuEI CTTGAG 2 cut(s) 341, 926
BpuMI CCSGG 1 cut(s) 873
BsaBI GATNNNNATC 1 cut(s) 968
BsaI GGTCTC 1 cut(s) 182
BsaJI CCNNGG 1 cut(s) 819
BsaXI ACNNNNNCTCC 2 cut(s) 737, 767
Bsc4I CCNNNNNNNGG 2 cut(s) 173, 468
Bse118I RCCGGY 1 cut(s) 886
Bse1I ACTGG 3 cut(s) 332, 390, 617
Bse8I GATNNNNATC 1 cut(s) 968
BseDI CCNNGG 1 cut(s) 819
BseGI GGATG 4 cut(s) 15, 259, 327, 732
BseJI GATNNNNATC 1 cut(s) 968
BseLI CCNNNNNNNGG 2 cut(s) 173, 468
BseMII CTCAG 2 cut(s) 458, 702
BseNI ACTGG 3 cut(s) 332, 390, 617
BseRI GAGGAG 2 cut(s) 1005, 1018
BseXI GCAGC 2 cut(s) 64, 463
BsgI GTGCAG 1 cut(s) 470
BshFI GGCC 2 cut(s) 232, 369
BsiSI CCGG 3 cut(s) 134, 872, 887
BslFI GGGAC 3 cut(s) 118, 744, 951
BslI CCNNNNNNNGG 2 cut(s) 173, 468
BsmAI GTCTC 3 cut(s) 104, 182, 376
BsmFI GGGAC 3 cut(s) 118, 744, 951
BsnI GGCC 2 cut(s) 232, 369
Bso31I GGTCTC 1 cut(s) 182
Bsp119I TTCGAA 1 cut(s) 702
Bsp143I GATC 4 cut(s) 47, 802, 924, 969
BspACI CCGC 1 cut(s) 251
BspANI GGCC 2 cut(s) 232, 369
BspCNI CTCAG 2 cut(s) 459, 703
BspLI GGNNCC 1 cut(s) 732
BspMI ACCTGC 1 cut(s) 70
BspOI GCTAGC 1 cut(s) 141
BspPI GGATC 2 cut(s) 55, 932
BspT104I TTCGAA 1 cut(s) 702
BspTI CTTAAG 1 cut(s) 628
BspTNI GGTCTC 1 cut(s) 182
BsrFI RCCGGY 1 cut(s) 886
BsrI ACTGG 3 cut(s) 332, 390, 617
BssAI RCCGGY 1 cut(s) 886
BssECI CCNNGG 1 cut(s) 819
BssMI GATC 4 cut(s) 47, 802, 924, 969
BssT1I CCWWGG 1 cut(s) 819
Bst4CI ACNGT 5 cut(s) 172, 226, 247, 351, 839
BstAFI CTTAAG 1 cut(s) 628
BstBI TTCGAA 1 cut(s) 702
BstC8I GCNNGC 3 cut(s) 139, 888, 990
BstDEI CTNAG 4 cut(s) 467, 596, 711, 996
BstF5I GGATG 4 cut(s) 15, 259, 327, 732
BstKTI GATC 4 cut(s) 50, 805, 927, 972
BstMAI GTCTC 3 cut(s) 104, 182, 376
BstMBI GATC 4 cut(s) 47, 802, 924, 969
BstMWI GCNNNNNNNGC 3 cut(s) 563, 824, 839
BstSCI CCNGG 1 cut(s) 871
BstSFI CTRYAG 1 cut(s) 246
BstV1I GCAGC 2 cut(s) 64, 463
BstV2I GAAGAC 2 cut(s) 691, 722
BstX2I RGATCY 1 cut(s) 924
BstYI RGATCY 1 cut(s) 924
BsuI GTATCC 2 cut(s) 860, 924
BsuRI GGCC 2 cut(s) 232, 369
BtrI CACGTC 1 cut(s) 265
BtsCI GGATG 4 cut(s) 15, 259, 327, 732
BtsIMutI CAGTG 5 cut(s) 168, 339, 464, 624, 844
BveI ACCTGC 1 cut(s) 70
Cac8I GCNNGC 3 cut(s) 139, 888, 990
CaiI CAGNNNCTG 1 cut(s) 77
Cfr10I RCCGGY 1 cut(s) 886
Cfr13I GGNCC 3 cut(s) 59, 230, 731
Csp6I GTAC 2 cut(s) 325, 592
CviQI GTAC 2 cut(s) 325, 592
DdeI CTNAG 4 cut(s) 467, 596, 711, 996
DpnI GATC 4 cut(s) 49, 804, 926, 971
DpnII GATC 4 cut(s) 47, 802, 924, 969
Eco130I CCWWGG 1 cut(s) 819
Eco31I GGTCTC 1 cut(s) 182
Eco32I GATATC 2 cut(s) 274, 896
Eco47I GGWCC 2 cut(s) 59, 731
EcoRV GATATC 2 cut(s) 274, 896
EcoT14I CCWWGG 1 cut(s) 819
ErhI CCWWGG 1 cut(s) 819
FaqI GGGAC 3 cut(s) 118, 744, 951
Fnu4HI GCNGC 2 cut(s) 78, 452
FokI GGATG 4 cut(s) 22, 266, 314, 739
Fsp4HI GCNGC 2 cut(s) 78, 452
FspBI CTAG 7 cut(s) 138, 183, 605, 774, 812, 846, 1019
GluI GCNGC 2 cut(s) 78, 452
GsuI CTGGAG 1 cut(s) 600
HaeIII GGCC 2 cut(s) 232, 369
HapII CCGG 3 cut(s) 134, 872, 887
HincII GTYRAC 1 cut(s) 493
HindII GTYRAC 1 cut(s) 493
HinfI GANTC 6 cut(s) 16, 195, 410, 530, 655, 939
HpaII CCGG 3 cut(s) 134, 872, 887
HphI GGTGA 1 cut(s) 649
Hpy166II GTNNAC 3 cut(s) 59, 262, 493
Hpy188I TCNGA 2 cut(s) 435, 952
Hpy188III TCNNGA 4 cut(s) 500, 527, 774, 787
Hpy8I GTNNAC 3 cut(s) 59, 262, 493
HpyCH4III ACNGT 5 cut(s) 172, 226, 247, 351, 839
HpyCH4IV ACGT 1 cut(s) 264
HpyCH4V TGCA 5 cut(s) 13, 65, 305, 451, 482
HpyF10VI GCNNNNNNNGC 3 cut(s) 563, 824, 839
HpyF3I CTNAG 4 cut(s) 467, 596, 711, 996
HpySE526I ACGT 1 cut(s) 264
KroI GCCGGC 1 cut(s) 886
KroNI GCCGGC 1 cut(s) 888
Kzo9I GATC 4 cut(s) 47, 802, 924, 969
LmnI GCTCC 1 cut(s) 125
Lsp1109I GCAGC 2 cut(s) 64, 463
LweI GCATC 6 cut(s) 168, 244, 326, 415, 544, 805
MaeI CTAG 7 cut(s) 138, 183, 605, 774, 812, 846, 1019
MaeII ACGT 1 cut(s) 264
MaeIII GTNAC 2 cut(s) 94, 599
MalI GATC 4 cut(s) 49, 804, 926, 971
MboI GATC 4 cut(s) 47, 802, 924, 969
MboII GAAGA 3 cut(s) 530, 691, 727
MfeI CAATTG 2 cut(s) 309, 858
MflI RGATCY 1 cut(s) 924
MluCI AATT 7 cut(s) 25, 309, 483, 548, 704, 757, 858
MlyI GAGTC 2 cut(s) 204, 933
MnlI CCTC 8 cut(s) 380, 462, 628, 739, 863, 996, 1023, 1026
MroNI GCCGGC 1 cut(s) 886
MroXI GAANNNNTTC 1 cut(s) 522
MseI TTAA 2 cut(s) 144, 629
MslI CAYNNNNRTG 3 cut(s) 332, 540, 881
MspCI CTTAAG 1 cut(s) 628
MspI CCGG 3 cut(s) 134, 872, 887
MspR9I CCNGG 1 cut(s) 873
MunI CAATTG 2 cut(s) 309, 858
MwoI GCNNNNNNNGC 3 cut(s) 563, 824, 839
NaeI GCCGGC 1 cut(s) 888
NciI CCSGG 1 cut(s) 873
NdeII GATC 4 cut(s) 47, 802, 924, 969
NgoMIV GCCGGC 1 cut(s) 886
NheI GCTAGC 1 cut(s) 137
NlaIV GGNNCC 1 cut(s) 732
NmuCI GTSAC 2 cut(s) 94, 599
NspV TTCGAA 1 cut(s) 702
PdiI GCCGGC 1 cut(s) 888
PdmI GAANNNNTTC 1 cut(s) 522
PfeI GAWTC 4 cut(s) 16, 410, 530, 655
PkrI GCNGC 2 cut(s) 79, 453
PleI GAGTC 2 cut(s) 203, 933
PpsI GAGTC 2 cut(s) 203, 933
PspN4I GGNNCC 1 cut(s) 732
PspPI GGNCC 3 cut(s) 59, 230, 731
PstNI CAGNNNCTG 1 cut(s) 77
PsuI RGATCY 1 cut(s) 924
RsaI GTAC 2 cut(s) 326, 593
RsaNI GTAC 2 cut(s) 325, 592
RseI CAYNNNNRTG 3 cut(s) 332, 540, 881
SaqAI TTAA 2 cut(s) 144, 629
SatI GCNGC 2 cut(s) 78, 452
Sau3AI GATC 4 cut(s) 47, 802, 924, 969
Sau96I GGNCC 3 cut(s) 59, 230, 731
SchI GAGTC 2 cut(s) 204, 933
ScrFI CCNGG 1 cut(s) 873
SfaNI GCATC 6 cut(s) 168, 244, 326, 415, 544, 805
SfcI CTRYAG 1 cut(s) 246
SfuI TTCGAA 1 cut(s) 702
SinI GGWCC 2 cut(s) 59, 731
SmiMI CAYNNNNRTG 3 cut(s) 332, 540, 881
SmlI CTYRAG 3 cut(s) 356, 628, 941
SmoI CTYRAG 3 cut(s) 356, 628, 941
SpeI ACTAGT 1 cut(s) 182
Sse9I AATT 7 cut(s) 25, 309, 483, 548, 704, 757, 858
SsiI CCGC 1 cut(s) 251
SspI AATATT 1 cut(s) 514
SspMI CTAG 7 cut(s) 138, 183, 605, 774, 812, 846, 1019
StyD4I CCNGG 1 cut(s) 871
StyI CCWWGG 1 cut(s) 819
TaaI ACNGT 5 cut(s) 172, 226, 247, 351, 839
TaiI ACGT 1 cut(s) 267
TaqI TCGA 2 cut(s) 187, 702
TaqII GACCGA 1 cut(s) 815
TasI AATT 7 cut(s) 25, 309, 483, 548, 704, 757, 858
TatI WGTACW 1 cut(s) 324
TfiI GAWTC 4 cut(s) 16, 410, 530, 655
Tru1I TTAA 2 cut(s) 144, 629
Tru9I TTAA 2 cut(s) 144, 629
TscAI CASTG 5 cut(s) 175, 339, 471, 624, 844
TseFI GTSAC 2 cut(s) 94, 599
TseI GCWGC 2 cut(s) 77, 451
Tsp45I GTSAC 2 cut(s) 94, 599
TspDTI ATGAA 2 cut(s) 18, 696
TspGWI ACGGA 1 cut(s) 122
TspRI CASTG 5 cut(s) 175, 339, 471, 624, 844
Vha464I CTTAAG 1 cut(s) 628
VpaK11BI GGWCC 2 cut(s) 59, 731
XapI RAATTY 2 cut(s) 548, 757
XbaI TCTAGA 1 cut(s) 773
XmnI GAANNNNTTC 1 cut(s) 522
XspI CTAG 7 cut(s) 138, 183, 605, 774, 812, 846, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.