Rmu_sc0002522.1_g000027

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002522.1
OG0000427
Physical Location & Seq
Reverse (-)
98873 .. 101121
2249 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002522.1_g000027.1.cds

Sequence Viewer

Length: 1485 bp
atggccaaaactccgttgatcagctcaaattcttgggttgttggtcactggcagtctggcactccattactgacattatcatccatggctaactatcttctcctctcgtttttgcttctaatttgcattctatcatatggctcagaaacactcacagccaaaaggcccaaaatcacaattgaagaagctactgttgatgatctccagcttgctttcaagctcaacactctgacctcaaggcaacttgttctgttctacatggacaagattcaaaaactgaacaaaaaccttagggctgtgatagaggggaaccctgatgctttatcccaagctgatatagctgacaaacaacacagagaatgcaaccacacactgcctaagcttcatggcattcctattctgctcaaggataccatagcaaccaaggacaaactcaacaccacagctggatcgtttcccttgttgggatccattgctcctcgagatgcaggtgttgtttctaagctaaggaggtctggagctatcattttagggaaggctagcttgagtgaatggtgccatttcaggtcattcctagcaccttctggatggagcgccagagaatccttacaattcatcggtggatcaagtagtggacctgcaatatcagtagctgctaatatggtttctgtgacattagggacggagactgacagctccattatctgtcctgctagctttaactctgtggtgggcatcaaacccactgttggtctcactagtcgagcaggagtcataccaatcagtccaagacaggacacagttgggtcaatttgtaggactatagcggatgctgttcacgtccttgatatcattgttggcaatgataggaatgataatgcaacaagtgaaacatcaaagtacatcccagtgggtggctatggacagtttctcaagcataatggcctgaaaggaaagagactggggatagtaaatatgttatttgattcaatcaaggacgatgcttttctgaaacaaacttttgtgcagcattttaccacactgaggaaacaagttgcagttttggttgacaatcttgaaatagccaatatagaagatgttctggattctcattcaagtggagaatttgatgcaatattagctgaattcaaaatagccttgaatgagtacctaagtgacctagtaaaatctccagtgaagtccttaagggaggtgatagagctcaacaagaatcattcaaaggtggaaaagatcaatgaatatgggcaagatgtcttcgaattagctgagaagactaatgggatgggaccaaaagagcaggaggtattgtcaaatttaaaaagactatctaaacaggggttcaagaagttcatgaccgatcattgcctagatgccttggtggcttatgctaacagtgcttctactatcctcgcaattggtggttttccgggcattgttgtgccggcaggatatcataacacttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

494

Amino Acids

53.62

Weight (kDa)

8.36

Isoelectric Point (pI)

38.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 479
Acc36I ACCTGC 2 cut(s) 479, 646
AccB1I GGYRCC 1 cut(s) 555
AccB7I CCANNNNNTGG 1 cut(s) 914
AciI CCGC 1 cut(s) 827
AclWI GGATC 4 cut(s) 457, 462, 475, 631
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 4 cut(s) 28, 1124, 1145, 1333
AfaI GTAC 2 cut(s) 902, 1169
AfiI CCNNNNNNNGG 4 cut(s) 464, 749, 914, 1044
AflII CTTAAG 1 cut(s) 1204
AhlI ACTAGT 1 cut(s) 758
AjiI CACGTC 1 cut(s) 841
Alw21I GWGCWC 1 cut(s) 1224
Alw26I GTCTC 3 cut(s) 680, 758, 952
AlwI GGATC 4 cut(s) 457, 462, 475, 631
AlwNI CAGNNNCTG 1 cut(s) 653
Ama87I CYCGRG 1 cut(s) 480
AoxI GGCC 3 cut(s) 3, 164, 943
ApeKI GCWGC 2 cut(s) 653, 1027
ApoI RAATTY 4 cut(s) 28, 1124, 1145, 1333
Asp700I GAANNNNTTC 1 cut(s) 1098
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 3 cut(s) 165, 635, 1307
AsuC2I CCSGG 1 cut(s) 1449
AsuHPI GGTGA 1 cut(s) 1225
AsuII TTCGAA 1 cut(s) 1278
AsuNHI GCTAGC 2 cut(s) 539, 713
AvaI CYCGRG 1 cut(s) 480
AvaII GGWCC 2 cut(s) 635, 1307
AxyI CCTNAGG 1 cut(s) 290
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 467
BanI GGYRCC 1 cut(s) 555
BanII GRGCYC 1 cut(s) 1224
BbsI GAAGAC 2 cut(s) 1267, 1298
Bbv12I GWGCWC 1 cut(s) 1224
BbvI GCAGC 2 cut(s) 640, 1039
BccI CCATC 2 cut(s) 582, 1297
BciVI GTATCC 1 cut(s) 403
BclI TGATCA 1 cut(s) 18
BcnI CCSGG 1 cut(s) 1449
BcoDI GTCTC 3 cut(s) 680, 758, 952
BcuI ACTAGT 1 cut(s) 758
BfaI CTAG 6 cut(s) 540, 575, 714, 759, 1181, 1388
BfmI CTRYAG 1 cut(s) 822
BfoI RGCGCY 1 cut(s) 597
BfrI CTTAAG 1 cut(s) 1204
BfuAI ACCTGC 2 cut(s) 479, 646
BfuI GTATCC 1 cut(s) 403
BglI GCCNNNNNGGC 1 cut(s) 1400
BisI GCNGC 2 cut(s) 654, 1028
BlsI GCNGC 2 cut(s) 655, 1029
Bme1390I CCNGG 1 cut(s) 1449
Bme18I GGWCC 2 cut(s) 635, 1307
BmeT110I CYCGRG 1 cut(s) 480
BmgBI CACGTC 1 cut(s) 841
BmgT120I GGNCC 3 cut(s) 165, 635, 1307
BmiI GGNNCC 4 cut(s) 311, 469, 557, 1308
BmrFI CCNGG 1 cut(s) 1449
BmrI ACTGGG 2 cut(s) 902, 971
BmsI GCATC 7 cut(s) 307, 475, 744, 820, 991, 1120, 1381
BmtI GCTAGC 2 cut(s) 543, 717
BmuI ACTGGG 2 cut(s) 902, 971
BpiI GAAGAC 2 cut(s) 1267, 1298
BpmI CTGGAG 3 cut(s) 188, 537, 1176
Bpu10I CCTNAGC 2 cut(s) 378, 506
Bpu14I TTCGAA 1 cut(s) 1278
BpuEI CTTGAG 4 cut(s) 220, 389, 565, 917
BpuMI CCSGG 1 cut(s) 1449
BsaI GGTCTC 1 cut(s) 758
BsaJI CCNNGG 3 cut(s) 84, 423, 1395
BsaXI ACNNNNNCTCC 2 cut(s) 1313, 1343
Bsc4I CCNNNNNNNGG 4 cut(s) 464, 749, 914, 1044
Bse118I RCCGGY 1 cut(s) 1462
Bse1I ACTGG 4 cut(s) 53, 908, 966, 1193
Bse21I CCTNAGG 1 cut(s) 290
Bse3DI GCAATG 3 cut(s) 471, 868, 1381
BseDI CCNNGG 3 cut(s) 84, 423, 1395
BseGI GGATG 5 cut(s) 80, 593, 835, 903, 1308
BseLI CCNNNNNNNGG 4 cut(s) 464, 749, 914, 1044
BseMI GCAATG 3 cut(s) 471, 868, 1381
BseMII CTCAG 3 cut(s) 156, 1034, 1278
BseNI ACTGG 4 cut(s) 53, 908, 966, 1193
BseRI GAGGAG 2 cut(s) 92, 468
BseXI GCAGC 2 cut(s) 640, 1039
BsgI GTGCAG 1 cut(s) 1046
BshFI GGCC 3 cut(s) 5, 166, 945
BshNI GGYRCC 1 cut(s) 555
BsiHKAI GWGCWC 1 cut(s) 1224
BsiHKCI CYCGRG 1 cut(s) 480
BsiSI CCGG 2 cut(s) 1448, 1463
BslFI GGGAC 2 cut(s) 694, 1320
BslI CCNNNNNNNGG 4 cut(s) 464, 749, 914, 1044
BsmAI GTCTC 3 cut(s) 680, 758, 952
BsmFI GGGAC 2 cut(s) 694, 1320
BsmI GAATGC 3 cut(s) 126, 365, 390
BsnI GGCC 3 cut(s) 5, 166, 945
Bso31I GGTCTC 1 cut(s) 758
BsoBI CYCGRG 1 cut(s) 480
Bsp119I TTCGAA 1 cut(s) 1278
Bsp1286I GDGCHC 1 cut(s) 1224
Bsp143I GATC 7 cut(s) 18, 199, 449, 467, 623, 1251, 1378
Bsp19I CCATGG 1 cut(s) 84
BspACI CCGC 1 cut(s) 827
BspANI GGCC 3 cut(s) 5, 166, 945
BspCNI CTCAG 3 cut(s) 155, 1035, 1279
BspHI TCATGA 1 cut(s) 1371
BspLI GGNNCC 4 cut(s) 311, 469, 557, 1308
BspMI ACCTGC 2 cut(s) 479, 646
BspOI GCTAGC 2 cut(s) 543, 717
BspPI GGATC 4 cut(s) 457, 462, 475, 631
BspT104I TTCGAA 1 cut(s) 1278
BspT107I GGYRCC 1 cut(s) 555
BspTI CTTAAG 1 cut(s) 1204
BspTNI GGTCTC 1 cut(s) 758
BsrDI GCAATG 3 cut(s) 471, 868, 1381
BsrFI RCCGGY 1 cut(s) 1462
BsrI ACTGG 4 cut(s) 53, 908, 966, 1193
BssAI RCCGGY 1 cut(s) 1462
BssECI CCNNGG 3 cut(s) 84, 423, 1395
BssMI GATC 7 cut(s) 18, 199, 449, 467, 623, 1251, 1378
BssT1I CCWWGG 3 cut(s) 84, 423, 1395
Bst4CI ACNGT 5 cut(s) 193, 748, 802, 927, 1415
BstAFI CTTAAG 1 cut(s) 1204
BstBI TTCGAA 1 cut(s) 1278
BstC8I GCNNGC 4 cut(s) 210, 541, 715, 1464
BstDEI CTNAG 8 cut(s) 142, 290, 378, 501, 506, 1043, 1172, 1287
BstDSI CCRYGG 1 cut(s) 84
BstF5I GGATG 5 cut(s) 80, 593, 835, 903, 1308
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 7 cut(s) 21, 202, 452, 470, 626, 1254, 1381
BstMAI GTCTC 3 cut(s) 680, 758, 952
BstMBI GATC 7 cut(s) 18, 199, 449, 467, 623, 1251, 1378
BstMWI GCNNNNNNNGC 4 cut(s) 338, 1139, 1400, 1415
BstSCI CCNGG 1 cut(s) 1447
BstSFI CTRYAG 1 cut(s) 822
BstV1I GCAGC 2 cut(s) 640, 1039
BstV2I GAAGAC 2 cut(s) 1267, 1298
BstX2I RGATCY 1 cut(s) 467
BstYI RGATCY 1 cut(s) 467
Bsu36I CCTNAGG 1 cut(s) 290
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 3 cut(s) 5, 166, 945
BtgI CCRYGG 1 cut(s) 84
BtrI CACGTC 1 cut(s) 841
BtsCI GGATG 5 cut(s) 80, 593, 835, 903, 1308
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 7 cut(s) 46, 371, 744, 915, 1040, 1200, 1420
BveI ACCTGC 2 cut(s) 479, 646
Cac8I GCNNGC 4 cut(s) 210, 541, 715, 1464
CaiI CAGNNNCTG 1 cut(s) 653
CciI TCATGA 1 cut(s) 1371
CfoI GCGC 1 cut(s) 596
Cfr10I RCCGGY 1 cut(s) 1462
Cfr13I GGNCC 3 cut(s) 165, 635, 1307
Csp6I GTAC 2 cut(s) 901, 1168
CviAII CATG 4 cut(s) 85, 259, 386, 1372
CviQI GTAC 2 cut(s) 901, 1168
DdeI CTNAG 8 cut(s) 142, 290, 378, 501, 506, 1043, 1172, 1287
DpnI GATC 7 cut(s) 20, 201, 451, 469, 625, 1253, 1380
DpnII GATC 7 cut(s) 18, 199, 449, 467, 623, 1251, 1378
DraI TTTAAA 1 cut(s) 1338
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 1222
Eco130I CCWWGG 3 cut(s) 84, 423, 1395
Eco24I GRGCYC 1 cut(s) 1224
Eco31I GGTCTC 1 cut(s) 758
Eco32I GATATC 2 cut(s) 850, 1472
Eco47I GGWCC 2 cut(s) 635, 1307
Eco53kI GAGCTC 1 cut(s) 1222
Eco81I CCTNAGG 1 cut(s) 290
Eco88I CYCGRG 1 cut(s) 480
EcoICRI GAGCTC 1 cut(s) 1222
EcoRI GAATTC 1 cut(s) 1145
EcoRV GATATC 2 cut(s) 850, 1472
EcoT14I CCWWGG 3 cut(s) 84, 423, 1395
EcoT38I GRGCYC 1 cut(s) 1224
ErhI CCWWGG 3 cut(s) 84, 423, 1395
FaeI CATG 4 cut(s) 88, 262, 389, 1375
FalI AAGNNNNNCTT 2 cut(s) 527, 559
FaqI GGGAC 2 cut(s) 694, 1320
FatI CATG 4 cut(s) 84, 258, 385, 1371
FauNDI CATATG 1 cut(s) 136
FbaI TGATCA 1 cut(s) 18
Fnu4HI GCNGC 2 cut(s) 654, 1028
FokI GGATG 5 cut(s) 67, 600, 842, 890, 1315
FriOI GRGCYC 1 cut(s) 1224
Fsp4HI GCNGC 2 cut(s) 654, 1028
FspBI CTAG 6 cut(s) 540, 575, 714, 759, 1181, 1388
GlaI GCGC 1 cut(s) 595
GluI GCNGC 2 cut(s) 654, 1028
GsuI CTGGAG 3 cut(s) 188, 537, 1176
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 3 cut(s) 5, 166, 945
HapII CCGG 2 cut(s) 1448, 1463
HhaI GCGC 1 cut(s) 596
Hin1II CATG 4 cut(s) 88, 262, 389, 1375
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HincII GTYRAC 1 cut(s) 1069
HindII GTYRAC 1 cut(s) 1069
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 6 cut(s) 268, 602, 771, 986, 1106, 1231
HpaII CCGG 2 cut(s) 1448, 1463
HphI GGTGA 1 cut(s) 1225
Hpy166II GTNNAC 3 cut(s) 635, 838, 1069
Hpy188I TCNGA 3 cut(s) 145, 231, 1011
Hpy188III TCNNGA 7 cut(s) 482, 516, 585, 1076, 1103, 1363, 1372
Hpy8I GTNNAC 3 cut(s) 635, 838, 1069
HpyAV CCTTC 2 cut(s) 529, 591
HpyCH4III ACNGT 5 cut(s) 193, 748, 802, 927, 1415
HpyCH4IV ACGT 1 cut(s) 840
HpyCH4V TGCA 8 cut(s) 126, 363, 488, 641, 881, 1027, 1058, 1133
HpyF10VI GCNNNNNNNGC 4 cut(s) 338, 1139, 1400, 1415
HpyF3I CTNAG 8 cut(s) 142, 290, 378, 501, 506, 1043, 1172, 1287
HpySE526I ACGT 1 cut(s) 840
Hsp92II CATG 4 cut(s) 88, 262, 389, 1375
HspAI GCGC 1 cut(s) 594
KroI GCCGGC 1 cut(s) 1462
KroNI GCCGGC 1 cut(s) 1464
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 7 cut(s) 18, 199, 449, 467, 623, 1251, 1378
LmnI GCTCC 4 cut(s) 481, 518, 591, 701
Lsp1109I GCAGC 2 cut(s) 640, 1039
LweI GCATC 7 cut(s) 307, 475, 744, 820, 991, 1120, 1381
MaeI CTAG 6 cut(s) 540, 575, 714, 759, 1181, 1388
MaeII ACGT 1 cut(s) 840
MaeIII GTNAC 3 cut(s) 44, 670, 1175
MalI GATC 7 cut(s) 20, 201, 451, 469, 625, 1253, 1380
MboI GATC 7 cut(s) 18, 199, 449, 467, 623, 1251, 1378
MboII GAAGA 5 cut(s) 89, 194, 1106, 1267, 1303
MfeI CAATTG 2 cut(s) 177, 1434
MflI RGATCY 1 cut(s) 467
MhlI GDGCHC 1 cut(s) 1224
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 780
MnlI CCTC 9 cut(s) 113, 244, 298, 489, 504, 1038, 1204, 1315, 1439
Mox20I TGGCCA 1 cut(s) 5
MroNI GCCGGC 1 cut(s) 1462
MroXI GAANNNNTTC 1 cut(s) 1098
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 720, 1205, 1337, 1483
MslI CAYNNNNRTG 3 cut(s) 908, 1116, 1457
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 446
MspCI CTTAAG 1 cut(s) 1204
MspI CCGG 2 cut(s) 1448, 1463
MspR9I CCNGG 1 cut(s) 1449
MunI CAATTG 2 cut(s) 177, 1434
Mva1269I GAATGC 3 cut(s) 126, 365, 390
MwoI GCNNNNNNNGC 4 cut(s) 338, 1139, 1400, 1415
NaeI GCCGGC 1 cut(s) 1464
NciI CCSGG 1 cut(s) 1449
NcoI CCATGG 1 cut(s) 84
NdeI CATATG 1 cut(s) 136
NdeII GATC 7 cut(s) 18, 199, 449, 467, 623, 1251, 1378
NgoMIV GCCGGC 1 cut(s) 1462
NheI GCTAGC 2 cut(s) 539, 713
NlaIII CATG 4 cut(s) 88, 262, 389, 1375
NlaIV GGNNCC 4 cut(s) 311, 469, 557, 1308
NmuCI GTSAC 3 cut(s) 44, 670, 1175
NspV TTCGAA 1 cut(s) 1278
PaeR7I CTCGAG 1 cut(s) 480
PagI TCATGA 1 cut(s) 1371
PaqCI CACCTGC 1 cut(s) 479
PctI GAATGC 3 cut(s) 126, 365, 390
PdiI GCCGGC 1 cut(s) 1464
PdmI GAANNNNTTC 1 cut(s) 1098
PfeI GAWTC 5 cut(s) 268, 602, 986, 1106, 1231
PflMI CCANNNNNTGG 1 cut(s) 914
PkrI GCNGC 2 cut(s) 655, 1029
PleI GAGTC 1 cut(s) 779
PpsI GAGTC 1 cut(s) 779
Psp124BI GAGCTC 1 cut(s) 1224
PspN4I GGNNCC 4 cut(s) 311, 469, 557, 1308
PspPI GGNCC 3 cut(s) 165, 635, 1307
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 1 cut(s) 467
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 2 cut(s) 902, 1169
RsaNI GTAC 2 cut(s) 901, 1168
RseI CAYNNNNRTG 3 cut(s) 908, 1116, 1457
SacI GAGCTC 1 cut(s) 1224
SaqAI TTAA 4 cut(s) 720, 1205, 1337, 1483
SatI GCNGC 2 cut(s) 654, 1028
Sau3AI GATC 7 cut(s) 18, 199, 449, 467, 623, 1251, 1378
Sau96I GGNCC 3 cut(s) 165, 635, 1307
SchI GAGTC 1 cut(s) 780
ScrFI CCNGG 1 cut(s) 1449
SduI GDGCHC 1 cut(s) 1224
SfaNI GCATC 7 cut(s) 307, 475, 744, 820, 991, 1120, 1381
SfcI CTRYAG 1 cut(s) 822
Sfr274I CTCGAG 1 cut(s) 480
SfuI TTCGAA 1 cut(s) 1278
SinI GGWCC 2 cut(s) 635, 1307
SlaI CTCGAG 1 cut(s) 480
SmiMI CAYNNNNRTG 3 cut(s) 908, 1116, 1457
SmlI CTYRAG 6 cut(s) 235, 404, 480, 544, 932, 1204
SmoI CTYRAG 6 cut(s) 235, 404, 480, 544, 932, 1204
SpeI ACTAGT 1 cut(s) 758
SsiI CCGC 1 cut(s) 827
SspI AATATT 1 cut(s) 1137
SspMI CTAG 6 cut(s) 540, 575, 714, 759, 1181, 1388
SstI GAGCTC 1 cut(s) 1224
StyD4I CCNGG 1 cut(s) 1447
StyI CCWWGG 3 cut(s) 84, 423, 1395
TaaI ACNGT 5 cut(s) 193, 748, 802, 927, 1415
TaiI ACGT 1 cut(s) 843
TaqI TCGA 3 cut(s) 481, 763, 1278
TaqII GACCGA 1 cut(s) 1391
TatI WGTACW 1 cut(s) 900
TfiI GAWTC 5 cut(s) 268, 602, 986, 1106, 1231
Tru1I TTAA 4 cut(s) 720, 1205, 1337, 1483
Tru9I TTAA 4 cut(s) 720, 1205, 1337, 1483
TscAI CASTG 7 cut(s) 53, 378, 751, 915, 1047, 1200, 1420
TseFI GTSAC 3 cut(s) 44, 670, 1175
TseI GCWGC 2 cut(s) 653, 1027
Tsp45I GTSAC 3 cut(s) 44, 670, 1175
TspDTI ATGAA 4 cut(s) 374, 604, 1272, 1360
TspGWI ACGGA 1 cut(s) 698
TspRI CASTG 7 cut(s) 53, 378, 751, 915, 1047, 1200, 1420
Van91I CCANNNNNTGG 1 cut(s) 914
Vha464I CTTAAG 1 cut(s) 1204
VpaK11BI GGWCC 2 cut(s) 635, 1307
XapI RAATTY 4 cut(s) 28, 1124, 1145, 1333
XhoI CTCGAG 1 cut(s) 480
XmnI GAANNNNTTC 1 cut(s) 1098
XspI CTAG 6 cut(s) 540, 575, 714, 759, 1181, 1388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.