Rmu_sc0002522.1_g000021

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002522.1
OG0000427
Physical Location & Seq
Reverse (-)
71596 .. 75413
3818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002522.1_g000021.1.cds

Sequence Viewer

Length: 1464 bp
atgaacccggatgcactttaccaagctgacaaggctgactatcagcgaaaggctaaggcccctggttattattccggcttgcatggcattcctgttttgctgaaggataacattggaaccagagataagctgaacactactgctggctcttttgcgctgctcggctctgtcgtgccccgggatgctggcgtggtgaccgagttgaggagcgctggagccattatcttggggaaggctagcttgagtgagtgggctcaggtcaggtctcttacgtcatccccaggtttcagccccagaggtggccaaggaaagaacccttatgtactatctgcaagtccatgtggatcaagcagtggatcttcaatatcagttgcagcaaatttggtagctgtatctcttggtactgagaccgatggctcaatactatgtccagcaagctttaactcagtagtcggcatcaaaccaaccgttggcctcacaagccgagccggggtgatcccagttactcccagacaggacaccattgggactgtgatagatgctgtttatgttcttgatgcgattgtaggctatgactacaaggatcaagcaaccacagaagcatcgaaatacattccacgaggtgggtataagcagttccttgggacttatgggctcaagggaaagagactgggaatagaaatgatcaaggaatttggccaagacatttttcttgcagcacaagccacaaatgggataggcaacaatgagagggcagcattgttaaatttagcaaagctgacaaaagatggatttgagaaactaatgacatacaacaggctagatgcattggtgactcccggtgcagatattgctcctgtacttgcaattgggggattcccaggcattagtgtccctgctggatatgacaggaagggtgtgcctttcggcataacttttgggggtctaaagggatcagaaccaaagcttatacagattgcctacggttctttctctctcagactcccaagccttccttcccctttcttcgatctcacaccctccgataagctcatatcgcacgccggcggtctcttccgtacttcgccgttccgcctcctctttcagattgagatcgacgagttagagcggcgtcgccaccttcctagctttgcctccgtagttgtcgttctcccttccctcctccgattctccaccaccttcaccgtcaccgacacttttgggagtccgaaacgatctctcggaattaggcaggagcttccataccggcctctcatttctctctgtcgccggaatctccaattggcttcgaacttgatgccggttcggaatggagcttcggaagacaacggcggcggttccgtgccaagaattaccgatgagattctcggcttcgtcgctccgatttcaaagcttccagactgttggaccggctttcctatacccactcgactacgatggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

487

Amino Acids

51.91

Weight (kDa)

9.49

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 470, 623
AccBSI CCGCTC 1 cut(s) 1129
AciI CCGC 5 cut(s) 1068, 1093, 1129, 1353, 1356
AclWI GGATC 5 cut(s) 352, 364, 490, 591, 961
AcoI YGGCCR 2 cut(s) 301, 697
AcsI RAATTY 3 cut(s) 379, 692, 766
AcuI CTGAAG 1 cut(s) 122
AcyI GRCGYC 1 cut(s) 1132
AdeI CACNNNGTG 1 cut(s) 623
AfaI GTAC 4 cut(s) 324, 403, 861, 1081
AfeI AGCGCT 1 cut(s) 211
AfiI CCNNNNNNNGG 6 cut(s) 204, 299, 470, 490, 623, 732
AgsI TTSAA 2 cut(s) 363, 1410
AjnI CCWGG 3 cut(s) 61, 280, 880
AjuI GAANNNNNNNTTGG 6 cut(s) 693, 725, 1000, 1032, 1286, 1318
Alw26I GTCTC 4 cut(s) 270, 401, 661, 1076
AlwI GGATC 5 cut(s) 352, 364, 490, 591, 961
Ama87I CYCGRG 1 cut(s) 177
Aor51HI AGCGCT 1 cut(s) 211
AoxI GGCC 5 cut(s) 57, 301, 472, 697, 1268
ApeKI GCWGC 4 cut(s) 157, 374, 716, 755
ApoI RAATTY 3 cut(s) 379, 692, 766
AspLEI GCGC 2 cut(s) 157, 212
AspS9I GGNCC 2 cut(s) 58, 1428
AsuC2I CCSGG 5 cut(s) 8, 178, 179, 490, 840
AsuHPI GGTGA 5 cut(s) 205, 505, 844, 1195, 1201
AsuII TTCGAA 1 cut(s) 1310
AsuNHI GCTAGC 1 cut(s) 236
AvaI CYCGRG 1 cut(s) 177
AvaII GGWCC 1 cut(s) 1428
BaeGI GKGCMC 1 cut(s) 177
BalI TGGCCA 2 cut(s) 303, 699
BanII GRGCYC 2 cut(s) 256, 657
BauI CACGAG 1 cut(s) 618
BbsI GAAGAC 1 cut(s) 1350
BbvI GCAGC 4 cut(s) 144, 386, 728, 767
BccI CCATC 3 cut(s) 407, 782, 1452
BceAI ACGGC 2 cut(s) 1072, 1366
BcgI CGANNNNNNTGC 2 cut(s) 1300, 1334
BciT130I CCWGG 3 cut(s) 63, 282, 882
BclI TGATCA 1 cut(s) 684
BcnI CCSGG 5 cut(s) 8, 178, 179, 490, 840
BcoDI GTCTC 4 cut(s) 270, 401, 661, 1076
BfaI CTAG 3 cut(s) 237, 821, 1146
BfoI RGCGCY 1 cut(s) 213
BisI GCNGC 6 cut(s) 158, 375, 717, 756, 1130, 1354
BlsI GCNGC 6 cut(s) 159, 376, 718, 757, 1131, 1355
Bme1390I CCNGG 8 cut(s) 8, 63, 178, 179, 282, 490, 840, 882
Bme18I GGWCC 1 cut(s) 1428
BmeT110I CYCGRG 1 cut(s) 177
BmgT120I GGNCC 2 cut(s) 58, 1428
BmiI GGNNCC 4 cut(s) 60, 118, 217, 1360
BmrFI CCNGG 8 cut(s) 8, 63, 178, 179, 282, 490, 840, 882
BmrI ACTGGG 2 cut(s) 494, 680
BmsI GCATC 7 cut(s) 172, 465, 529, 547, 611, 814, 1308
BmtI GCTAGC 1 cut(s) 240
BmuI ACTGGG 2 cut(s) 494, 680
BpiI GAAGAC 1 cut(s) 1350
BpmI CTGGAG 1 cut(s) 234
Bpu10I CCTNAGC 2 cut(s) 54, 255
Bpu14I TTCGAA 1 cut(s) 1310
BpuEI CTTGAG 2 cut(s) 262, 641
BpuMI CCSGG 5 cut(s) 8, 178, 179, 490, 840
BsaBI GATNNNNATC 1 cut(s) 1112
BsaHI GRCGYC 1 cut(s) 1132
BsaI GGTCTC 3 cut(s) 270, 401, 1076
BsaJI CCNNGG 8 cut(s) 61, 176, 177, 280, 304, 489, 640, 880
Bsc4I CCNNNNNNNGG 6 cut(s) 204, 299, 470, 490, 623, 732
Bse118I RCCGGY 4 cut(s) 1064, 1266, 1321, 1430
Bse1I ACTGG 2 cut(s) 500, 675
Bse8I GATNNNNATC 1 cut(s) 1112
BseBI CCWGG 3 cut(s) 63, 282, 882
BseDI CCNNGG 8 cut(s) 61, 176, 177, 280, 304, 489, 640, 880
BseGI GGATG 3 cut(s) 16, 187, 275
BseJI GATNNNNATC 1 cut(s) 1112
BseLI CCNNNNNNNGG 6 cut(s) 204, 299, 470, 490, 623, 732
BseMII CTCAG 4 cut(s) 269, 396, 459, 1012
BseNI ACTGG 2 cut(s) 500, 675
BseRI GAGGAG 3 cut(s) 220, 1088, 1172
BseSI GKGCMC 1 cut(s) 177
BseXI GCAGC 4 cut(s) 144, 386, 728, 767
BsgI GTGCAG 1 cut(s) 864
BshFI GGCC 5 cut(s) 59, 303, 474, 699, 1270
BsiHKCI CYCGRG 1 cut(s) 177
BslFI GGGAC 3 cut(s) 541, 658, 878
BslI CCNNNNNNNGG 6 cut(s) 204, 299, 470, 490, 623, 732
BsmAI GTCTC 4 cut(s) 270, 401, 661, 1076
BsmFI GGGAC 3 cut(s) 541, 658, 878
BsmI GAATGC 1 cut(s) 87
BsnI GGCC 5 cut(s) 59, 303, 474, 699, 1270
Bso31I GGTCTC 3 cut(s) 270, 401, 1076
BsoBI CYCGRG 1 cut(s) 177
Bsp119I TTCGAA 1 cut(s) 1310
Bsp1286I GDGCHC 3 cut(s) 177, 256, 657
Bsp143I GATC 9 cut(s) 344, 356, 495, 583, 684, 953, 1030, 1113, 1235
BspACI CCGC 5 cut(s) 1068, 1093, 1129, 1353, 1356
BspANI GGCC 5 cut(s) 59, 303, 474, 699, 1270
BspCNI CTCAG 4 cut(s) 268, 397, 458, 1011
BspLI GGNNCC 4 cut(s) 60, 118, 217, 1360
BspOI GCTAGC 1 cut(s) 240
BspPI GGATC 5 cut(s) 352, 364, 490, 591, 961
BspT104I TTCGAA 1 cut(s) 1310
BspTNI GGTCTC 3 cut(s) 270, 401, 1076
BsrBI CCGCTC 1 cut(s) 1129
BsrFI RCCGGY 4 cut(s) 1064, 1266, 1321, 1430
BsrI ACTGG 2 cut(s) 500, 675
BssAI RCCGGY 4 cut(s) 1064, 1266, 1321, 1430
BssECI CCNNGG 8 cut(s) 61, 176, 177, 280, 304, 489, 640, 880
BssMI GATC 9 cut(s) 344, 356, 495, 583, 684, 953, 1030, 1113, 1235
BssNI GRCGYC 1 cut(s) 1132
BssSI CACGAG 1 cut(s) 618
BssT1I CCWWGG 2 cut(s) 304, 640
Bst2BI CACGAG 1 cut(s) 618
Bst2UI CCWGG 3 cut(s) 63, 282, 882
Bst4CI ACNGT 5 cut(s) 469, 532, 986, 1207, 1424
Bst6I CTCTTC 1 cut(s) 1079
BstACI GRCGYC 1 cut(s) 1132
BstAPI GCANNNNNTGC 1 cut(s) 851
BstBI TTCGAA 1 cut(s) 1310
BstC8I GCNNGC 7 cut(s) 80, 145, 187, 238, 436, 1062, 1066
BstDEI CTNAG 5 cut(s) 54, 255, 405, 445, 998
BstEII GGTNACC 1 cut(s) 193
BstF5I GGATG 3 cut(s) 16, 187, 275
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 2 cut(s) 157, 212
BstKTI GATC 9 cut(s) 347, 359, 498, 586, 687, 956, 1033, 1116, 1238
BstMAI GTCTC 4 cut(s) 270, 401, 661, 1076
BstMBI GATC 9 cut(s) 344, 356, 495, 583, 684, 953, 1030, 1113, 1235
BstMWI GCNNNNNNNGC 6 cut(s) 32, 84, 480, 722, 851, 1057
BstNI CCWGG 3 cut(s) 63, 282, 882
BstPI GGTNACC 1 cut(s) 193
BstSCI CCNGG 8 cut(s) 6, 61, 176, 177, 280, 488, 838, 880
BstSLI GKGCMC 1 cut(s) 177
BstV1I GCAGC 4 cut(s) 144, 386, 728, 767
BstV2I GAAGAC 1 cut(s) 1350
BstX2I RGATCY 1 cut(s) 356
BstXI CCANNNNNNTGG 2 cut(s) 226, 1425
BstYI RGATCY 1 cut(s) 356
BsuRI GGCC 5 cut(s) 59, 303, 474, 699, 1270
BtsCI GGATG 3 cut(s) 16, 187, 275
BtsI GCAGTG 1 cut(s) 358
BtsIMutI CAGTG 1 cut(s) 358
Cac8I GCNNGC 7 cut(s) 80, 145, 187, 238, 436, 1062, 1066
CfoI GCGC 2 cut(s) 157, 212
Cfr10I RCCGGY 4 cut(s) 1064, 1266, 1321, 1430
Cfr13I GGNCC 2 cut(s) 58, 1428
Cfr9I CCCGGG 1 cut(s) 177
CseI GACGC 1 cut(s) 1121
Csp6I GTAC 4 cut(s) 323, 402, 860, 1080
CviAII CATG 2 cut(s) 83, 339
CviQI GTAC 4 cut(s) 323, 402, 860, 1080
DdeI CTNAG 5 cut(s) 54, 255, 405, 445, 998
DpnI GATC 9 cut(s) 346, 358, 497, 585, 686, 955, 1032, 1115, 1237
DpnII GATC 9 cut(s) 344, 356, 495, 583, 684, 953, 1030, 1113, 1235
DraIII CACNNNGTG 1 cut(s) 623
EaeI YGGCCR 2 cut(s) 301, 697
Eam1104I CTCTTC 1 cut(s) 1079
EarI CTCTTC 1 cut(s) 1079
EciI GGCGGA 1 cut(s) 1082
Eco130I CCWWGG 2 cut(s) 304, 640
Eco24I GRGCYC 2 cut(s) 256, 657
Eco31I GGTCTC 3 cut(s) 270, 401, 1076
Eco47I GGWCC 1 cut(s) 1428
Eco47III AGCGCT 1 cut(s) 211
Eco57I CTGAAG 1 cut(s) 122
Eco88I CYCGRG 1 cut(s) 177
Eco91I GGTNACC 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 58
EcoO65I GGTNACC 1 cut(s) 193
EcoRII CCWGG 3 cut(s) 61, 280, 880
EcoT14I CCWWGG 2 cut(s) 304, 640
EcoT22I ATGCAT 1 cut(s) 829
EcoT38I GRGCYC 2 cut(s) 256, 657
ErhI CCWWGG 2 cut(s) 304, 640
FaeI CATG 2 cut(s) 86, 342
FalI AAGNNNNNCTT 4 cut(s) 224, 256, 1000, 1032
FaqI GGGAC 3 cut(s) 541, 658, 878
FatI CATG 2 cut(s) 82, 338
FbaI TGATCA 1 cut(s) 684
Fnu4HI GCNGC 6 cut(s) 158, 375, 717, 756, 1130, 1354
FokI GGATG 3 cut(s) 23, 194, 262
FriOI GRGCYC 2 cut(s) 256, 657
Fsp4HI GCNGC 6 cut(s) 158, 375, 717, 756, 1130, 1354
FspBI CTAG 3 cut(s) 237, 821, 1146
GlaI GCGC 2 cut(s) 156, 211
GluI GCNGC 6 cut(s) 158, 375, 717, 756, 1130, 1354
GsuI CTGGAG 1 cut(s) 234
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 5 cut(s) 59, 303, 474, 699, 1270
HgaI GACGC 1 cut(s) 1121
HhaI GCGC 2 cut(s) 157, 212
Hin1I GRCGYC 1 cut(s) 1132
Hin1II CATG 2 cut(s) 86, 342
Hin6I GCGC 2 cut(s) 155, 210
HinP1I GCGC 2 cut(s) 155, 210
HindIII AAGCTT 3 cut(s) 436, 965, 1412
HinfI GANTC 7 cut(s) 835, 876, 1002, 1188, 1225, 1294, 1384
HphI GGTGA 5 cut(s) 205, 505, 844, 1195, 1201
Hpy188III TCNNGA 2 cut(s) 554, 1418
Hpy99I CGWCG 3 cut(s) 1121, 1137, 1400
HpyAV CCTTC 8 cut(s) 97, 226, 907, 1022, 1026, 1151, 1185, 1210
HpyCH4III ACNGT 5 cut(s) 469, 532, 986, 1207, 1424
HpyCH4IV ACGT 1 cut(s) 272
HpyCH4V TGCA 8 cut(s) 14, 82, 332, 374, 716, 827, 845, 866
HpyF10VI GCNNNNNNNGC 6 cut(s) 32, 84, 480, 722, 851, 1057
HpyF3I CTNAG 5 cut(s) 54, 255, 405, 445, 998
HpySE526I ACGT 1 cut(s) 272
Hsp92I GRCGYC 1 cut(s) 1132
Hsp92II CATG 2 cut(s) 86, 342
HspAI GCGC 2 cut(s) 155, 210
KroI GCCGGC 1 cut(s) 1064
KroNI GCCGGC 1 cut(s) 1066
Ksp22I TGATCA 1 cut(s) 684
Kzo9I GATC 9 cut(s) 344, 356, 495, 583, 684, 953, 1030, 1113, 1235
LmnI GCTCC 6 cut(s) 207, 215, 859, 1255, 1334, 1405
Lsp1109I GCAGC 4 cut(s) 144, 386, 728, 767
LweI GCATC 7 cut(s) 172, 465, 529, 547, 611, 814, 1308
MaeI CTAG 3 cut(s) 237, 821, 1146
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 4 cut(s) 193, 502, 832, 1207
MalI GATC 9 cut(s) 346, 358, 497, 585, 686, 955, 1032, 1115, 1237
MbiI CCGCTC 1 cut(s) 1129
MboI GATC 9 cut(s) 344, 356, 495, 583, 684, 953, 1030, 1113, 1235
MboII GAAGA 4 cut(s) 351, 1018, 1066, 1355
MfeI CAATTG 2 cut(s) 867, 1301
MflI RGATCY 1 cut(s) 356
MhlI GDGCHC 3 cut(s) 177, 256, 657
MlsI TGGCCA 2 cut(s) 303, 699
MluCI AATT 7 cut(s) 379, 692, 766, 867, 1245, 1301, 1371
MluNI TGGCCA 2 cut(s) 303, 699
MlyI GAGTC 3 cut(s) 829, 996, 1234
MmeI TCCRAC 1 cut(s) 1406
Mox20I TGGCCA 2 cut(s) 303, 699
Mph1103I ATGCAT 1 cut(s) 829
MreI CGCCGGCG 1 cut(s) 1064
MroNI GCCGGC 1 cut(s) 1064
MscI TGGCCA 2 cut(s) 303, 699
MseI TTAA 2 cut(s) 441, 764
Msp20I TGGCCA 2 cut(s) 303, 699
MspR9I CCNGG 8 cut(s) 8, 63, 178, 179, 282, 490, 840, 882
MunI CAATTG 2 cut(s) 867, 1301
Mva1269I GAATGC 1 cut(s) 87
MvaI CCWGG 3 cut(s) 63, 282, 882
MwoI GCNNNNNNNGC 6 cut(s) 32, 84, 480, 722, 851, 1057
NaeI GCCGGC 1 cut(s) 1066
NciI CCSGG 5 cut(s) 8, 178, 179, 490, 840
NdeII GATC 9 cut(s) 344, 356, 495, 583, 684, 953, 1030, 1113, 1235
NgoMIV GCCGGC 1 cut(s) 1064
NheI GCTAGC 1 cut(s) 236
NlaIII CATG 2 cut(s) 86, 342
NlaIV GGNNCC 4 cut(s) 60, 118, 217, 1360
NmeAIII GCCGAG 3 cut(s) 141, 509, 1368
NmuCI GTSAC 3 cut(s) 193, 832, 1207
NsiI ATGCAT 1 cut(s) 829
NspV TTCGAA 1 cut(s) 1310
PcsI WCGNNNNNNNCGW 1 cut(s) 168
PctI GAATGC 1 cut(s) 87
PdiI GCCGGC 1 cut(s) 1066
PfeI GAWTC 4 cut(s) 876, 1188, 1294, 1384
PflMI CCANNNNNTGG 2 cut(s) 470, 623
PkrI GCNGC 6 cut(s) 159, 376, 718, 757, 1131, 1355
PleI GAGTC 3 cut(s) 829, 996, 1233
PpsI GAGTC 3 cut(s) 829, 996, 1233
Psp6I CCWGG 3 cut(s) 61, 280, 880
PspEI GGTNACC 1 cut(s) 193
PspGI CCWGG 3 cut(s) 61, 280, 880
PspN4I GGNNCC 4 cut(s) 60, 118, 217, 1360
PspPI GGNCC 2 cut(s) 58, 1428
PsrI GAACNNNNNNTAC 4 cut(s) 620, 652, 1152, 1184
PsuI RGATCY 1 cut(s) 356
RsaI GTAC 4 cut(s) 324, 403, 861, 1081
RsaNI GTAC 4 cut(s) 323, 402, 860, 1080
SaqAI TTAA 2 cut(s) 441, 764
SatI GCNGC 6 cut(s) 158, 375, 717, 756, 1130, 1354
Sau3AI GATC 9 cut(s) 344, 356, 495, 583, 684, 953, 1030, 1113, 1235
Sau96I GGNCC 2 cut(s) 58, 1428
SchI GAGTC 3 cut(s) 829, 996, 1234
ScrFI CCNGG 8 cut(s) 8, 63, 178, 179, 282, 490, 840, 882
SduI GDGCHC 3 cut(s) 177, 256, 657
SfaNI GCATC 7 cut(s) 172, 465, 529, 547, 611, 814, 1308
SfuI TTCGAA 1 cut(s) 1310
SgrAI CRCCGGYG 1 cut(s) 1064
SinI GGWCC 1 cut(s) 1428
SmaI CCCGGG 1 cut(s) 179
SmlI CTYRAG 2 cut(s) 241, 656
SmoI CTYRAG 2 cut(s) 241, 656
Sse9I AATT 7 cut(s) 379, 692, 766, 867, 1245, 1301, 1371
SsiI CCGC 5 cut(s) 1068, 1093, 1129, 1353, 1356
SspMI CTAG 3 cut(s) 237, 821, 1146
StyD4I CCNGG 8 cut(s) 6, 61, 176, 177, 280, 488, 838, 880
StyI CCWWGG 2 cut(s) 304, 640
TaaI ACNGT 5 cut(s) 469, 532, 986, 1207, 1424
TaiI ACGT 1 cut(s) 275
TaqI TCGA 5 cut(s) 605, 1029, 1116, 1310, 1450
TaqII GACCGA 2 cut(s) 212, 425
TasI AATT 7 cut(s) 379, 692, 766, 867, 1245, 1301, 1371
TatI WGTACW 2 cut(s) 322, 859
TauI GCSGC 2 cut(s) 1132, 1356
TfiI GAWTC 4 cut(s) 876, 1188, 1294, 1384
Tru1I TTAA 2 cut(s) 441, 764
Tru9I TTAA 2 cut(s) 441, 764
TscAI CASTG 1 cut(s) 358
TseFI GTSAC 3 cut(s) 193, 832, 1207
TseI GCWGC 4 cut(s) 157, 374, 716, 755
Tsp45I GTSAC 3 cut(s) 193, 832, 1207
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 3 cut(s) 1067, 1147, 1351
TspMI CCCGGG 1 cut(s) 177
TspRI CASTG 1 cut(s) 358
Van91I CCANNNNNTGG 2 cut(s) 470, 623
VpaK11BI GGWCC 1 cut(s) 1428
XapI RAATTY 3 cut(s) 379, 692, 766
XmaI CCCGGG 1 cut(s) 177
XspI CTAG 3 cut(s) 237, 821, 1146
Zsp2I ATGCAT 1 cut(s) 829
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.