Rh6BG021100

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000427
Physical Location & Seq
Reverse (-)
3129949 .. 3140176
10228 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG021100.1

Sequence Viewer

Length: 1521 bp
ATGACGAAACCAAGCTTCCCTCACATCTTATCTTTATTGCTTCTCATTCTTCTCATCACATTATCATCTCTGCCAGGCACATTCGCAGCCCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTCCAGCTCGCTTTTAAGCACAACCAACTCACCTCGAGGCAACTTGTCCAATATTACCTTTCCCAAATCCGAAAACTTAACCCGGTTCTCAAAGGAGTCCTAGAAGTCAACCCGGATGCGCTTCACCTTGCCGACAAGGCTGACGATGAGCGCAGGACCAAGGCATCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCAACCAAGGATAAGATGAACACTACCGCCGGCTCTTTTGCGCTTCTTGGATCTGTCGTCCCTCGTGATGCCGGAGTGGTGGAGAAGTTGAGGAAAGAAGGGGCCATTATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCAAATTCTAGGTCTAATAATACAGCACCAAATGGTTGGAGCGCCAGAGGCGGTTATGGCTTGAACCCTTACACATTCTCAGACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTAGCAGCAAATCTGGCAGCGGTGTCATTAGGTACCGAGACAGATGGTTCAATCTTATGTCCATCAGGTTACAACTCGGTTGTGGGCATCAAACCAACAATCGGTCTTACTAGTCGAGCAGGAGTTGTCCCAATTTCTCCAAGACAGGATACTGTTGGGCCAATTTGTAGGACAGTAGCAGATGCTACTTATGTTCTTGATGCCATAGCAGGAATCGACCACAATGACATTGCAACGATTGAGACATCAAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCGCAACGGACTTAGAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATGACTTTGGCAATGACACTTCCTTGGCTCAAACTTTTGAGAAACATCTCAACACTCTGAGGAAAGGAGGTGCTGTTTTGGTAGACAATTTGGAAATTGCTAATTTGACTGAAATCTATTCTTCAAGCGATGAAGATACTGCATTGTTTGTTGAGTACAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCCGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACGTATTTTTAGCTGTTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCGGGCATTGTTGAATCTTGCAAGGTGGACAAGAAATGGTCTTGTAAAATTGGTGACAGAGAGGAAGCTAGATGCTGTGAACGCAGCGCAGCCAAGGAAGAGTGGAAAGACGAGTGTGTGGGTGCGTTGCTTGGCTGGTGGATCGACGAGGTCGAAGACGGCTTGGATTTACGGCCAGCAGAGGAATGTCTCGGCTCCTCAGTTGCTACGTCTCTTTTTCCATGACTGCTTCATTGAGAGAGACAGGTATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

506

Amino Acids

55.09

Weight (kDa)

9.57

Isoelectric Point (pI)

34.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 57 - 353 4.1e-68 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 160
Acc65I GGTACC 1 cut(s) 635
AccB1I GGYRCC 1 cut(s) 635
AccI GTMKAC 1 cut(s) 1020
AciI CCGC 7 cut(s) 91, 375, 537, 576, 588, 623, 1267
AclWI GGATC 3 cut(s) 406, 1140, 1414
AcoI YGGCCR 1 cut(s) 1438
AcsI RAATTY 1 cut(s) 490
AfaI GTAC 3 cut(s) 637, 857, 1094
AfiI CCNNNNNNNGG 3 cut(s) 704, 869, 1189
AgsI TTSAA 7 cut(s) 550, 654, 1062, 1120, 1171, 1235, 1279
AhlI ACTAGT 1 cut(s) 713
AjnI CCWGG 1 cut(s) 73
AluBI AGCT 5 cut(s) 15, 133, 474, 1229, 1333
AluI AGCT 5 cut(s) 15, 133, 474, 1229, 1333
Alw26I GTCTC 5 cut(s) 635, 839, 1459, 1481, 1500
AlwI GGATC 3 cut(s) 406, 1140, 1414
Ama87I CYCGRG 1 cut(s) 160
AoxI GGCC 3 cut(s) 450, 761, 1438
ApeKI GCWGC 6 cut(s) 86, 585, 608, 620, 1349, 1354
ApoI RAATTY 1 cut(s) 490
ArsI GACNNNNNNTTYG 2 cut(s) 482, 514
Asp718I GGTACC 1 cut(s) 635
AspLEI GCGC 6 cut(s) 247, 279, 391, 530, 894, 1354
AspS9I GGNCC 3 cut(s) 282, 450, 761
AsuC2I CCSGG 2 cut(s) 209, 239
AsuHPI GGTGA 3 cut(s) 148, 242, 1330
AsuNHI GCTAGC 1 cut(s) 470
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 1 cut(s) 282
BanI GGYRCC 1 cut(s) 635
BauI CACGAG 1 cut(s) 411
BbsI GAAGAC 1 cut(s) 1427
BbvI GCAGC 6 cut(s) 98, 597, 620, 632, 1361, 1366
BccI CCATC 2 cut(s) 641, 673
BceAI ACGGC 2 cut(s) 1440, 1453
BciT130I CCWGG 1 cut(s) 75
BciVI GTATCC 1 cut(s) 745
BcnI CCSGG 2 cut(s) 209, 239
BcoDI GTCTC 5 cut(s) 635, 839, 1459, 1481, 1500
BcuI ACTAGT 1 cut(s) 713
BfaI CTAG 5 cut(s) 227, 471, 495, 714, 1334
BfoI RGCGCY 1 cut(s) 531
BfuI GTATCC 1 cut(s) 745
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 7 cut(s) 87, 577, 586, 609, 621, 1350, 1355
BlsI GCNGC 7 cut(s) 88, 578, 587, 610, 622, 1351, 1356
Bme1390I CCNGG 3 cut(s) 75, 209, 239
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 3 cut(s) 282, 450, 761
BmiI GGNNCC 3 cut(s) 451, 637, 1461
BmrFI CCNGG 3 cut(s) 75, 209, 239
BmrI ACTGGG 1 cut(s) 1133
BmsI GCATC 7 cut(s) 232, 299, 406, 699, 775, 793, 1327
BmtI GCTAGC 1 cut(s) 474
BmuI ACTGGG 1 cut(s) 1133
BpiI GAAGAC 1 cut(s) 1427
BpmI CTGGAG 1 cut(s) 113
BpuEI CTTGAG 2 cut(s) 496, 565
BpuMI CCSGG 2 cut(s) 209, 239
BsaJI CCNNGG 5 cut(s) 285, 354, 960, 1149, 1358
BsaXI ACNNNNNCTCC 2 cut(s) 996, 1026
Bsc4I CCNNNNNNNGG 3 cut(s) 704, 869, 1189
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 2 cut(s) 1139, 1194
Bse3DI GCAATG 3 cut(s) 345, 831, 955
BseBI CCWGG 1 cut(s) 75
BseDI CCNNGG 5 cut(s) 285, 354, 960, 1149, 1358
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 3 cut(s) 704, 869, 1189
BseMI GCAATG 3 cut(s) 345, 831, 955
BseMII CTCAG 4 cut(s) 579, 901, 986, 1478
BseNI ACTGG 2 cut(s) 1139, 1194
BseRI GAGGAG 1 cut(s) 1452
BseXI GCAGC 6 cut(s) 98, 597, 620, 632, 1361, 1366
BshFI GGCC 3 cut(s) 452, 763, 1440
BshNI GGYRCC 1 cut(s) 635
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 4 cut(s) 209, 239, 378, 420
BslFI GGGAC 2 cut(s) 392, 716
BslI CCNNNNNNNGG 3 cut(s) 704, 869, 1189
BsmAI GTCTC 5 cut(s) 635, 839, 1459, 1481, 1500
BsmBI CGTCTC 1 cut(s) 1481
BsmFI GGGAC 2 cut(s) 392, 716
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 3 cut(s) 452, 763, 1440
BsoBI CYCGRG 1 cut(s) 160
Bsp143I GATC 5 cut(s) 124, 398, 1145, 1197, 1406
BspACI CCGC 7 cut(s) 91, 375, 537, 576, 588, 623, 1267
BspANI GGCC 3 cut(s) 452, 763, 1440
BspCNI CTCAG 4 cut(s) 578, 900, 987, 1477
BspLI GGNNCC 3 cut(s) 451, 637, 1461
BspOI GCTAGC 1 cut(s) 474
BspPI GGATC 3 cut(s) 406, 1140, 1414
BspT107I GGYRCC 1 cut(s) 635
BsrDI GCAATG 3 cut(s) 345, 831, 955
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 2 cut(s) 1139, 1194
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 5 cut(s) 285, 354, 960, 1149, 1358
BssMI GATC 5 cut(s) 124, 398, 1145, 1197, 1406
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 5 cut(s) 285, 354, 960, 1149, 1358
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 1 cut(s) 75
Bst4CI ACNGT 3 cut(s) 757, 778, 882
Bst6I CTCTTC 1 cut(s) 1358
BstC8I GCNNGC 6 cut(s) 91, 135, 379, 472, 1269, 1442
BstDEI CTNAG 6 cut(s) 309, 565, 887, 902, 995, 1464
BstF5I GGATG 1 cut(s) 247
BstH2I RGCGCY 1 cut(s) 531
BstHHI GCGC 6 cut(s) 247, 279, 391, 530, 894, 1354
BstKTI GATC 5 cut(s) 127, 401, 1148, 1200, 1409
BstMAI GTCTC 5 cut(s) 635, 839, 1459, 1481, 1500
BstMBI GATC 5 cut(s) 124, 398, 1145, 1197, 1406
BstMWI GCNNNNNNNGC 8 cut(s) 263, 534, 543, 582, 585, 617, 1235, 1346
BstNI CCWGG 1 cut(s) 75
BstSCI CCNGG 3 cut(s) 73, 207, 237
BstV1I GCAGC 6 cut(s) 98, 597, 620, 632, 1361, 1366
BstV2I GAAGAC 1 cut(s) 1427
BstX2I RGATCY 1 cut(s) 398
BstXI CCANNNNNNTGG 2 cut(s) 460, 522
BstYI RGATCY 1 cut(s) 398
BsuI GTATCC 1 cut(s) 745
BsuRI GGCC 3 cut(s) 452, 763, 1440
BtgZI GCGATG 1 cut(s) 1080
BtsCI GGATG 1 cut(s) 247
BtsIMutI CAGTG 2 cut(s) 300, 1146
Cac8I GCNNGC 6 cut(s) 91, 135, 379, 472, 1269, 1442
CfoI GCGC 6 cut(s) 247, 279, 391, 530, 894, 1354
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 3 cut(s) 282, 450, 761
Csp6I GTAC 3 cut(s) 636, 856, 1093
CviAII CATG 3 cut(s) 317, 1487, 1518
CviQI GTAC 3 cut(s) 636, 856, 1093
DdeI CTNAG 6 cut(s) 309, 565, 887, 902, 995, 1464
DpnI GATC 5 cut(s) 126, 400, 1147, 1199, 1408
DpnII GATC 5 cut(s) 124, 398, 1145, 1197, 1406
EaeI YGGCCR 1 cut(s) 1438
Eam1104I CTCTTC 1 cut(s) 1358
EarI CTCTTC 1 cut(s) 1358
Eco130I CCWWGG 5 cut(s) 285, 354, 960, 1149, 1358
Eco47I GGWCC 1 cut(s) 282
Eco88I CYCGRG 1 cut(s) 160
EcoRII CCWGG 1 cut(s) 73
EcoT14I CCWWGG 5 cut(s) 285, 354, 960, 1149, 1358
EcoT22I ATGCAT 2 cut(s) 1114, 1519
ErhI CCWWGG 5 cut(s) 285, 354, 960, 1149, 1358
Esp3I CGTCTC 1 cut(s) 1481
FaeI CATG 3 cut(s) 320, 1490, 1521
FalI AAGNNNNNCTT 2 cut(s) 458, 490
FaqI GGGAC 2 cut(s) 392, 716
FatI CATG 3 cut(s) 316, 1486, 1517
FauI CCCGC 2 cut(s) 98, 1260
FblI GTMKAC 1 cut(s) 1020
Fnu4HI GCNGC 7 cut(s) 87, 577, 586, 609, 621, 1350, 1355
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 7 cut(s) 87, 577, 586, 609, 621, 1350, 1355
FspBI CTAG 5 cut(s) 227, 471, 495, 714, 1334
GlaI GCGC 6 cut(s) 246, 278, 390, 529, 893, 1353
GluI GCNGC 7 cut(s) 87, 577, 586, 609, 621, 1350, 1355
GsuI CTGGAG 1 cut(s) 113
HaeII RGCGCY 1 cut(s) 531
HaeIII GGCC 3 cut(s) 452, 763, 1440
HapII CCGG 4 cut(s) 209, 239, 378, 420
HhaI GCGC 6 cut(s) 247, 279, 391, 530, 894, 1354
Hin1II CATG 3 cut(s) 320, 1490, 1521
Hin6I GCGC 6 cut(s) 245, 277, 389, 528, 892, 1352
HinP1I GCGC 6 cut(s) 245, 277, 389, 528, 892, 1352
HincII GTYRAC 1 cut(s) 235
HindII GTYRAC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 4 cut(s) 96, 222, 816, 1279
HpaII CCGG 4 cut(s) 209, 239, 378, 420
HphI GGTGA 3 cut(s) 148, 242, 1330
Hpy166II GTNNAC 4 cut(s) 235, 1021, 1293, 1345
Hpy188I TCNGA 3 cut(s) 197, 568, 996
Hpy188III TCNNGA 2 cut(s) 413, 800
Hpy8I GTNNAC 4 cut(s) 235, 1021, 1293, 1345
Hpy99I CGWCG 1 cut(s) 1414
HpyAV CCTTC 4 cut(s) 440, 943, 1114, 1177
HpyCH4III ACNGT 3 cut(s) 757, 778, 882
HpyCH4IV ACGT 2 cut(s) 1218, 1474
HpyCH4V TGCA 8 cut(s) 316, 350, 836, 878, 1079, 1112, 1286, 1517
HpyF10VI GCNNNNNNNGC 8 cut(s) 263, 534, 543, 582, 585, 617, 1235, 1346
HpyF3I CTNAG 6 cut(s) 309, 565, 887, 902, 995, 1464
HpySE526I ACGT 2 cut(s) 1218, 1474
Hsp92II CATG 3 cut(s) 320, 1490, 1521
HspAI GCGC 6 cut(s) 245, 277, 389, 528, 892, 1352
KpnI GGTACC 1 cut(s) 639
KroI GCCGGC 1 cut(s) 377
KroNI GCCGGC 1 cut(s) 379
Kzo9I GATC 5 cut(s) 124, 398, 1145, 1197, 1406
LmnI GCTCC 2 cut(s) 525, 1465
Lsp1109I GCAGC 6 cut(s) 98, 597, 620, 632, 1361, 1366
LweI GCATC 7 cut(s) 232, 299, 406, 699, 775, 793, 1327
MaeI CTAG 5 cut(s) 227, 471, 495, 714, 1334
MaeII ACGT 2 cut(s) 1218, 1474
MaeIII GTNAC 3 cut(s) 297, 671, 1318
MalI GATC 5 cut(s) 126, 400, 1147, 1199, 1408
MboI GATC 5 cut(s) 124, 398, 1145, 1197, 1406
MboII GAAGA 5 cut(s) 41, 1050, 1082, 1375, 1432
MflI RGATCY 1 cut(s) 398
MluCI AATT 7 cut(s) 490, 735, 765, 1024, 1032, 1039, 1313
MlyI GAGTC 2 cut(s) 90, 231
MmeI TCCRAC 1 cut(s) 503
Mph1103I ATGCAT 2 cut(s) 1114, 1519
MroNI GCCGGC 1 cut(s) 377
MseI TTAA 3 cut(s) 141, 204, 1106
MspA1I CMGCKG 2 cut(s) 588, 623
MspI CCGG 4 cut(s) 209, 239, 378, 420
MspR9I CCNGG 3 cut(s) 75, 209, 239
Mva1269I GAATGC 1 cut(s) 321
MvaI CCWGG 1 cut(s) 75
MwoI GCNNNNNNNGC 8 cut(s) 263, 534, 543, 582, 585, 617, 1235, 1346
NaeI GCCGGC 1 cut(s) 379
NciI CCSGG 2 cut(s) 209, 239
NdeII GATC 5 cut(s) 124, 398, 1145, 1197, 1406
NgoMIV GCCGGC 1 cut(s) 377
NheI GCTAGC 1 cut(s) 470
NlaIII CATG 3 cut(s) 320, 1490, 1521
NlaIV GGNNCC 3 cut(s) 451, 637, 1461
NmeAIII GCCGAG 1 cut(s) 1436
NmuCI GTSAC 2 cut(s) 297, 1318
NsiI ATGCAT 2 cut(s) 1114, 1519
PaeR7I CTCGAG 1 cut(s) 160
PcsI WCGNNNNNNNCGW 1 cut(s) 1415
PctI GAATGC 1 cut(s) 321
PdiI GCCGGC 1 cut(s) 379
PfeI GAWTC 2 cut(s) 816, 1279
PflFI GACNNNGTC 1 cut(s) 1414
PkrI GCNGC 7 cut(s) 88, 578, 587, 610, 622, 1351, 1356
PleI GAGTC 2 cut(s) 90, 230
PpsI GAGTC 2 cut(s) 90, 230
Psp6I CCWGG 1 cut(s) 73
PspGI CCWGG 1 cut(s) 73
PspN4I GGNNCC 3 cut(s) 451, 637, 1461
PspPI GGNCC 3 cut(s) 282, 450, 761
PspXI VCTCGAGB 1 cut(s) 160
PsuI RGATCY 1 cut(s) 398
PsyI GACNNNGTC 1 cut(s) 1414
RsaI GTAC 3 cut(s) 637, 857, 1094
RsaNI GTAC 3 cut(s) 636, 856, 1093
SaqAI TTAA 3 cut(s) 141, 204, 1106
SatI GCNGC 7 cut(s) 87, 577, 586, 609, 621, 1350, 1355
Sau3AI GATC 5 cut(s) 124, 398, 1145, 1197, 1406
Sau96I GGNCC 3 cut(s) 282, 450, 761
SchI GAGTC 2 cut(s) 90, 231
ScrFI CCNGG 3 cut(s) 75, 209, 239
SfaNI GCATC 7 cut(s) 232, 299, 406, 699, 775, 793, 1327
Sfr274I CTCGAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 282
SlaI CTCGAG 1 cut(s) 160
SmlI CTYRAG 3 cut(s) 160, 475, 580
SmoI CTYRAG 3 cut(s) 160, 475, 580
SpeI ACTAGT 1 cut(s) 713
Sse9I AATT 7 cut(s) 490, 735, 765, 1024, 1032, 1039, 1313
SsiI CCGC 7 cut(s) 91, 375, 537, 576, 588, 623, 1267
SspI AATATT 1 cut(s) 179
SspMI CTAG 5 cut(s) 227, 471, 495, 714, 1334
StyD4I CCNGG 3 cut(s) 73, 207, 237
StyI CCWWGG 5 cut(s) 285, 354, 960, 1149, 1358
TaaI ACNGT 3 cut(s) 757, 778, 882
TaiI ACGT 2 cut(s) 1221, 1477
TaqI TCGA 5 cut(s) 161, 718, 819, 1409, 1418
TaqII GACCGA 1 cut(s) 695
TasI AATT 7 cut(s) 490, 735, 765, 1024, 1032, 1039, 1313
TatI WGTACW 2 cut(s) 855, 1092
TauI GCSGC 1 cut(s) 579
TfiI GAWTC 2 cut(s) 816, 1279
Tru1I TTAA 3 cut(s) 141, 204, 1106
Tru9I TTAA 3 cut(s) 141, 204, 1106
TscAI CASTG 2 cut(s) 300, 1146
TseFI GTSAC 2 cut(s) 297, 1318
TseI GCWGC 6 cut(s) 86, 585, 608, 620, 1349, 1354
Tsp45I GTSAC 2 cut(s) 297, 1318
TspDTI ATGAA 3 cut(s) 380, 1083, 1486
TspGWI ACGGA 1 cut(s) 912
TspRI CASTG 2 cut(s) 300, 1146
Tth111I GACNNNGTC 1 cut(s) 1414
VpaK11BI GGWCC 1 cut(s) 282
XapI RAATTY 1 cut(s) 490
XhoI CTCGAG 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 1020
XspI CTAG 5 cut(s) 227, 471, 495, 714, 1334
Zsp2I ATGCAT 2 cut(s) 1114, 1519
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.