Rroxscaffold_7G00215610

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000427
Physical Location & Seq
Forward (+)
66212040 .. 66214131
2092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00215610.1

Sequence Viewer

Length: 972 bp
ATGGAGGTGAACCCTGATGCTTTATCCCAAGCTGATATAGCTGACAAACAACGCAGAGAATGCAACCACACACTGCCTAAGCTTCATGGCATTCCTATTCTGCTCAAGGATACCATAGCAACCAAGGACAAACTCAACACCACAGCTGGCTCGTTTGCCTTTGTTGGGGTCATTCCTAGCACCTTCTGGATGGAGCGCCAGAGGTTCCCAAGGGATGGGACGGAGACTGACAGCTCCATTATCTGTCCGGCTAGCTTTAACTCTGTGGTGGGCATCAAACCCACTGTTGGTCTCACTAGTCGAGCAGGAGTCATACCAATCAGTCCAAGACAGGACACAGTTGGGCCAATTTGTAGGACTGTAGTGGATGCTGTTCACGTCCTTGATATCATTGTTGGCATTGATAGGAATGATAATGCAACAAGTGAAGCATCAAAGTATATCCCAGTGGGTGGCTATGGACGGTTTCTCAAGCATAATGGCCTCAAAGGAAAGAGACTGGGGATAGTAAATATGTTATTTGATTCAATCAAGGACGATGCTTTTCTGAAACAGACTTCTAAACAAGGTGCAGCTTTGGTTGACAATCTTGAAGTAGCCAATATTGAAGATGTTCTGGATTCTCATTCAAGTGGAATGTTTGAAGCAATATTAGCTGAATTCAAAATAGCCTTGAATGAGTACCTAAGTGACCTAGTAAAATCTCCAGTGAAGTCCTTAAGGGAGGTGATAGAGTTCAACAAGAATCATTCAAAGGAGGAAAAGATCAACAAATATGGGCAAGATGTCTTCGAATTAGCTGAGAAGACTAATGGGATGGGACCAAAAGAGCAGGAGGATATCATAACACTAAAAAAGTATCCTTTTGGGATCTGCTTTGGGGGACTCAAGGGTTCAGAGCCAAAGCTGATTGAGATCGCCTATGCCTTTGAGCAAGCAACTAAGATAAGGAGAAAGCCTCCTCTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

323

Amino Acids

35.54

Weight (kDa)

7.02

Isoelectric Point (pI)

36.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 3 - 59 9.4e-09 Amidase
Amidase PF01425 73 - 249 5.7e-23 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 215, 452
AclWI GGATC 1 cut(s) 878
AcsI RAATTY 1 cut(s) 659
AfaI GTAC 1 cut(s) 683
AfiI CCNNNNNNNGG 4 cut(s) 165, 215, 287, 452
AflII CTTAAG 1 cut(s) 718
AgsI TTSAA 9 cut(s) 528, 593, 608, 630, 644, 664, 676, 739, 753
AhlI ACTAGT 1 cut(s) 296
AjiI CACGTC 1 cut(s) 379
Alw26I GTCTC 3 cut(s) 218, 296, 490
AlwI GGATC 1 cut(s) 878
AoxI GGCC 2 cut(s) 344, 481
ApeKI GCWGC 1 cut(s) 572
ApoI RAATTY 1 cut(s) 659
Asp700I GAANNNNTTC 1 cut(s) 612
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 2 cut(s) 344, 821
AsuHPI GGTGA 2 cut(s) 19, 739
AsuII TTCGAA 1 cut(s) 792
AsuNHI GCTAGC 1 cut(s) 251
AvaII GGWCC 1 cut(s) 821
BbsI GAAGAC 2 cut(s) 781, 812
BbvI GCAGC 1 cut(s) 584
BccI CCATC 3 cut(s) 184, 209, 811
BciVI GTATCC 2 cut(s) 103, 870
BcoDI GTCTC 3 cut(s) 218, 296, 490
BcuI ACTAGT 1 cut(s) 296
BfaI CTAG 5 cut(s) 177, 252, 297, 695, 965
BfmI CTRYAG 1 cut(s) 360
BfoI RGCGCY 1 cut(s) 199
BfrI CTTAAG 1 cut(s) 718
BfuI GTATCC 2 cut(s) 103, 870
BisI GCNGC 1 cut(s) 573
BlsI GCNGC 1 cut(s) 574
Bme18I GGWCC 1 cut(s) 821
BmgBI CACGTC 1 cut(s) 379
BmgT120I GGNCC 2 cut(s) 344, 821
BmiI GGNNCC 2 cut(s) 206, 822
BmrI ACTGGG 2 cut(s) 440, 509
BmsI GCATC 5 cut(s) 7, 282, 358, 440, 529
BmtI GCTAGC 1 cut(s) 255
BmuI ACTGGG 2 cut(s) 440, 509
BpiI GAAGAC 2 cut(s) 781, 812
BplI GAGNNNNNCTC 1 cut(s) 943
BpmI CTGGAG 1 cut(s) 690
Bpu10I CCTNAGC 1 cut(s) 78
Bpu14I TTCGAA 1 cut(s) 792
BpuEI CTTGAG 3 cut(s) 89, 455, 872
BsaBI GATNNNNATC 1 cut(s) 914
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 2 cut(s) 123, 209
Bsc4I CCNNNNNNNGG 4 cut(s) 165, 215, 287, 452
Bse1I ACTGG 3 cut(s) 446, 504, 707
Bse8I GATNNNNATC 1 cut(s) 914
BseDI CCNNGG 2 cut(s) 123, 209
BseGI GGATG 4 cut(s) 195, 220, 373, 822
BseJI GATNNNNATC 1 cut(s) 914
BseLI CCNNNNNNNGG 4 cut(s) 165, 215, 287, 452
BseMII CTCAG 1 cut(s) 792
BseNI ACTGG 3 cut(s) 446, 504, 707
BseRI GAGGAG 1 cut(s) 951
BseXI GCAGC 1 cut(s) 584
BsgI GTGCAG 1 cut(s) 591
BshFI GGCC 2 cut(s) 346, 483
BsiSI CCGG 1 cut(s) 248
BslFI GGGAC 3 cut(s) 232, 834, 897
BslI CCNNNNNNNGG 4 cut(s) 165, 215, 287, 452
BsmAI GTCTC 3 cut(s) 218, 296, 490
BsmFI GGGAC 3 cut(s) 232, 834, 897
BsmI GAATGC 2 cut(s) 65, 90
BsnI GGCC 2 cut(s) 346, 483
Bso31I GGTCTC 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 792
Bsp143I GATC 3 cut(s) 765, 870, 915
BspANI GGCC 2 cut(s) 346, 483
BspCNI CTCAG 1 cut(s) 793
BspLI GGNNCC 2 cut(s) 206, 822
BspOI GCTAGC 1 cut(s) 255
BspPI GGATC 1 cut(s) 878
BspT104I TTCGAA 1 cut(s) 792
BspTI CTTAAG 1 cut(s) 718
BspTNI GGTCTC 1 cut(s) 296
BsrI ACTGG 3 cut(s) 446, 504, 707
BssECI CCNNGG 2 cut(s) 123, 209
BssMI GATC 3 cut(s) 765, 870, 915
BssT1I CCWWGG 2 cut(s) 123, 209
Bst4CI ACNGT 4 cut(s) 286, 340, 361, 465
BstAFI CTTAAG 1 cut(s) 718
BstAPI GCANNNNNTGC 1 cut(s) 60
BstBI TTCGAA 1 cut(s) 792
BstC8I GCNNGC 3 cut(s) 148, 253, 936
BstDEI CTNAG 4 cut(s) 78, 686, 801, 942
BstF5I GGATG 4 cut(s) 195, 220, 373, 822
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 3 cut(s) 768, 873, 918
BstMAI GTCTC 3 cut(s) 218, 296, 490
BstMBI GATC 3 cut(s) 765, 870, 915
BstMWI GCNNNNNNNGC 3 cut(s) 38, 60, 653
BstSFI CTRYAG 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 584
BstV2I GAAGAC 2 cut(s) 781, 812
BstX2I RGATCY 1 cut(s) 870
BstYI RGATCY 1 cut(s) 870
BsuI GTATCC 2 cut(s) 103, 870
BsuRI GGCC 2 cut(s) 346, 483
BtrI CACGTC 1 cut(s) 379
BtsCI GGATG 4 cut(s) 195, 220, 373, 822
BtsI GCAGTG 1 cut(s) 71
BtsIMutI CAGTG 4 cut(s) 71, 282, 453, 714
Cac8I GCNNGC 3 cut(s) 148, 253, 936
CfoI GCGC 1 cut(s) 198
Cfr13I GGNCC 2 cut(s) 344, 821
Csp6I GTAC 1 cut(s) 682
CviAII CATG 1 cut(s) 86
CviQI GTAC 1 cut(s) 682
DdeI CTNAG 4 cut(s) 78, 686, 801, 942
DpnI GATC 3 cut(s) 767, 872, 917
DpnII GATC 3 cut(s) 765, 870, 915
Eco130I CCWWGG 2 cut(s) 123, 209
Eco31I GGTCTC 1 cut(s) 296
Eco32I GATATC 2 cut(s) 388, 841
Eco47I GGWCC 1 cut(s) 821
EcoRI GAATTC 1 cut(s) 659
EcoRV GATATC 2 cut(s) 388, 841
EcoT14I CCWWGG 2 cut(s) 123, 209
ErhI CCWWGG 2 cut(s) 123, 209
FaeI CATG 1 cut(s) 89
FaqI GGGAC 3 cut(s) 232, 834, 897
FatI CATG 1 cut(s) 85
Fnu4HI GCNGC 1 cut(s) 573
FokI GGATG 4 cut(s) 202, 227, 380, 829
Fsp4HI GCNGC 1 cut(s) 573
FspBI CTAG 5 cut(s) 177, 252, 297, 695, 965
GlaI GCGC 1 cut(s) 197
GluI GCNGC 1 cut(s) 573
GsuI CTGGAG 1 cut(s) 690
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 2 cut(s) 346, 483
HapII CCGG 1 cut(s) 248
HhaI GCGC 1 cut(s) 198
Hin1II CATG 1 cut(s) 89
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 5 cut(s) 309, 524, 620, 745, 885
HpaII CCGG 1 cut(s) 248
HphI GGTGA 2 cut(s) 19, 739
Hpy166II GTNNAC 3 cut(s) 10, 376, 583
Hpy188I TCNGA 2 cut(s) 549, 898
Hpy188III TCNNGA 3 cut(s) 187, 590, 617
Hpy8I GTNNAC 3 cut(s) 10, 376, 583
HpyAV CCTTC 1 cut(s) 193
HpyCH4III ACNGT 4 cut(s) 286, 340, 361, 465
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 3 cut(s) 63, 419, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 60, 653
HpyF3I CTNAG 4 cut(s) 78, 686, 801, 942
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 1 cut(s) 89
HspAI GCGC 1 cut(s) 196
Kzo9I GATC 3 cut(s) 765, 870, 915
LmnI GCTCC 2 cut(s) 193, 239
Lsp1109I GCAGC 1 cut(s) 584
LweI GCATC 5 cut(s) 7, 282, 358, 440, 529
MaeI CTAG 5 cut(s) 177, 252, 297, 695, 965
MaeII ACGT 1 cut(s) 378
MaeIII GTNAC 1 cut(s) 689
MalI GATC 3 cut(s) 767, 872, 917
MboI GATC 3 cut(s) 765, 870, 915
MboII GAAGA 3 cut(s) 620, 781, 817
MflI RGATCY 1 cut(s) 870
MluCI AATT 3 cut(s) 348, 659, 794
MlyI GAGTC 2 cut(s) 318, 879
MnlI CCTC 7 cut(s) 195, 494, 718, 751, 829, 969, 972
MroXI GAANNNNTTC 1 cut(s) 612
MseI TTAA 2 cut(s) 258, 719
MslI CAYNNNNRTG 1 cut(s) 630
MspA1I CMGCKG 1 cut(s) 146
MspCI CTTAAG 1 cut(s) 718
MspI CCGG 1 cut(s) 248
Mva1269I GAATGC 2 cut(s) 65, 90
MwoI GCNNNNNNNGC 3 cut(s) 38, 60, 653
NdeII GATC 3 cut(s) 765, 870, 915
NheI GCTAGC 1 cut(s) 251
NlaIII CATG 1 cut(s) 89
NlaIV GGNNCC 2 cut(s) 206, 822
NmuCI GTSAC 1 cut(s) 689
NspV TTCGAA 1 cut(s) 792
PctI GAATGC 2 cut(s) 65, 90
PdmI GAANNNNTTC 1 cut(s) 612
PfeI GAWTC 3 cut(s) 524, 620, 745
PflMI CCANNNNNTGG 2 cut(s) 215, 452
PkrI GCNGC 1 cut(s) 574
PleI GAGTC 2 cut(s) 317, 879
PpsI GAGTC 2 cut(s) 317, 879
PspN4I GGNNCC 2 cut(s) 206, 822
PspPI GGNCC 2 cut(s) 344, 821
PsuI RGATCY 1 cut(s) 870
PvuII CAGCTG 1 cut(s) 146
RsaI GTAC 1 cut(s) 683
RsaNI GTAC 1 cut(s) 682
RseI CAYNNNNRTG 1 cut(s) 630
SaqAI TTAA 2 cut(s) 258, 719
SatI GCNGC 1 cut(s) 573
Sau3AI GATC 3 cut(s) 765, 870, 915
Sau96I GGNCC 2 cut(s) 344, 821
SchI GAGTC 2 cut(s) 318, 879
SfaNI GCATC 5 cut(s) 7, 282, 358, 440, 529
SfcI CTRYAG 1 cut(s) 360
SfuI TTCGAA 1 cut(s) 792
SinI GGWCC 1 cut(s) 821
SmiMI CAYNNNNRTG 1 cut(s) 630
SmlI CTYRAG 4 cut(s) 104, 470, 718, 887
SmoI CTYRAG 4 cut(s) 104, 470, 718, 887
SpeI ACTAGT 1 cut(s) 296
Sse9I AATT 3 cut(s) 348, 659, 794
SspI AATATT 2 cut(s) 604, 651
SspMI CTAG 5 cut(s) 177, 252, 297, 695, 965
StyI CCWWGG 2 cut(s) 123, 209
TaaI ACNGT 4 cut(s) 286, 340, 361, 465
TaiI ACGT 1 cut(s) 381
TaqI TCGA 2 cut(s) 301, 792
TasI AATT 3 cut(s) 348, 659, 794
TfiI GAWTC 3 cut(s) 524, 620, 745
Tru1I TTAA 2 cut(s) 258, 719
Tru9I TTAA 2 cut(s) 258, 719
TscAI CASTG 4 cut(s) 78, 289, 453, 714
TseFI GTSAC 1 cut(s) 689
TseI GCWGC 1 cut(s) 572
Tsp45I GTSAC 1 cut(s) 689
TspDTI ATGAA 1 cut(s) 74
TspGWI ACGGA 1 cut(s) 236
TspRI CASTG 4 cut(s) 78, 289, 453, 714
Van91I CCANNNNNTGG 2 cut(s) 215, 452
Vha464I CTTAAG 1 cut(s) 718
VpaK11BI GGWCC 1 cut(s) 821
XapI RAATTY 1 cut(s) 659
XmnI GAANNNNTTC 1 cut(s) 612
XspI CTAG 5 cut(s) 177, 252, 297, 695, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.