Rw6G002150

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000427
Physical Location & Seq
Reverse (-)
3463774 .. 3465890
2117 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002150.1

Sequence Viewer

Length: 1515 bp
ATGGCAAAACCAAGCTTCCCTCACATCTTATCTTCATTGCTTCTCATTCTTTTCTTCACATTTTCATCTCTGCCAGGCACATTCGCAGCTCGCAGACTCTCACTCAAAGAAGCCACCATAGACGATCTTCAGCTCGCTTTTAAGCACAACCAACTCACCTCGAGGCAGCTTGTCCAATATTACCATTCCCAAATCCGAAAACTGAACCCGGTTCTCAAAGGAGTCCTAGAAGTAAACCCGGATGCGCTTCACCTTGCCGACAAGGCTGACTACGAGCGCAGGACCAAGGCACCAGTGTCACTACTCTCTAAGTTGCATGGCATTCCTATTCTGGTCAAGGACAACATTGCAACCAAGGATAAGATGAACACCACCGCCGGCTCTTTTGCACTGCTTGGATCTGTCGTGCCTCCTGGGGCTATCATTTTGGGAAAGGCTAGCTTGAGCGAGTGGTCACATTTTAGGTCTAATGAAGCACCCTCCGGTTGGAACGCCAGAGGCGGTTATGGCTTGAACCCTTACACATTCTCAAACCCTTGCGGCTCAAGCAGCGGTTCAGCAATATCAGTGGCAGCAAATCTGGCAGCGGTGTCATTAGGCACTGAGACAGATGGTTCAATCTTATGTCCATCAGGTTATAACTCAGTTGTGGGCATCAAACCAACAATCGGTCTTACTAGTCGAGCAGGGGTTGTCCCAATTTCTCCAAGACAGGATACTGTCGGGCCAATTTGTAGGACAGTAGCAGATGCTGCGTATGTTCTTGATGCCATAGCAGGCATCGACCACAATGACATTGCAACGATTGAGACATCAAAGTACGTTCCCAAAGGTGGTTATGCAATGTTTCTCAGGCGCGACGGACTTAAAGGAAAAAGAATAGGGATATTGAGAGCCTTCTATAACTTCGGCAATGACACTGCCTTGGCTCAAACGTTTGAGAAACATCTCAACACTCTGAGGAAAGGAGGTGCTGTTTTGGTAGACAATTTAGAAATTGCTAATTTAACTGAAATCTATTCTTCAAGCGATGAAGATACTGCATTGTCTGTTGAGTTCAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGTTATAGCCTTCAACAACAAACACCAGAAACTGGAAAAGATCAAGGAGTATGGGCAAGACCTATTTTTAGCAGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCGGGAATTGTTGAATCTTGCGAGGTGGACAAGAAATGGTCTTGTGAAATTGGTGACAGAGAAGAAGCTAGATGCTGTGGTGACTCCTAAAGCAGATTTTTCAAAGATACTTGCAATTGGGGGAGCCCCAGGTCTAATAGTTCCAGCCGGATACGCGAACAATGGGAGACCAGGTGGTATATGCTTTGGTGGACTTCGGGGTTCAGAGCCAAAGCTAATTGAGATTGCCTATGCTTTTGAGCAAGCTACTAAGATTAGAAAGCCTCCTTCATTCAAAGGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

504

Amino Acids

54.23

Weight (kDa)

9.44

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 57 - 439 2e-57 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 639
AbsI CCTCGAGG 1 cut(s) 160
AccB1I GGYRCC 1 cut(s) 289
AccB7I CCANNNNNTGG 1 cut(s) 1153
AccI GTMKAC 1 cut(s) 984
AccII CGCG 2 cut(s) 858, 1385
AciI CCGC 6 cut(s) 375, 501, 540, 552, 587, 1231
AclI AACGTT 1 cut(s) 935
AclWI GGATC 2 cut(s) 406, 1104
AcuI CTGAAG 2 cut(s) 113, 1218
AfaI GTAC 1 cut(s) 821
AfiI CCNNNNNNNGG 4 cut(s) 486, 668, 833, 1153
AgsI TTSAA 9 cut(s) 514, 618, 1026, 1060, 1084, 1135, 1243, 1332, 1504
AhlI ACTAGT 1 cut(s) 677
AjnI CCWGG 4 cut(s) 73, 412, 1357, 1399
AluBI AGCT 9 cut(s) 15, 89, 133, 169, 441, 1196, 1297, 1444, 1475
AluI AGCT 9 cut(s) 15, 89, 133, 169, 441, 1196, 1297, 1444, 1475
Alw26I GTCTC 3 cut(s) 599, 803, 1390
AlwI GGATC 2 cut(s) 406, 1104
AlwNI CAGNNNCTG 2 cut(s) 752, 1153
Ama87I CYCGRG 1 cut(s) 160
AoxI GGCC 1 cut(s) 725
ApeKI GCWGC 7 cut(s) 86, 166, 549, 572, 584, 752, 1193
AspLEI GCGC 3 cut(s) 247, 279, 858
AspS9I GGNCC 2 cut(s) 282, 725
AsuC2I CCSGG 2 cut(s) 209, 239
AsuHPI GGTGA 4 cut(s) 148, 242, 1294, 1321
AsuNHI GCTAGC 1 cut(s) 437
AvaI CYCGRG 1 cut(s) 160
AvaII GGWCC 1 cut(s) 282
BanI GGYRCC 1 cut(s) 289
BanII GRGCYC 1 cut(s) 1357
BbvI GCAGC 7 cut(s) 98, 178, 561, 584, 596, 739, 1205
BccI CCATC 2 cut(s) 605, 637
BciT130I CCWGG 4 cut(s) 75, 414, 1359, 1401
BciVI GTATCC 2 cut(s) 709, 1373
BcnI CCSGG 2 cut(s) 209, 239
BcoDI GTCTC 3 cut(s) 599, 803, 1390
BcuI ACTAGT 1 cut(s) 677
BfaI CTAG 5 cut(s) 227, 438, 678, 1298, 1513
BfuI GTATCC 2 cut(s) 709, 1373
BglI GCCNNNNNGGC 1 cut(s) 263
BisI GCNGC 8 cut(s) 87, 167, 541, 550, 573, 585, 753, 1194
BlsI GCNGC 8 cut(s) 88, 168, 542, 551, 574, 586, 754, 1195
Bme1390I CCNGG 6 cut(s) 75, 209, 239, 414, 1359, 1401
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 160
BmgT120I GGNCC 2 cut(s) 282, 725
BmiI GGNNCC 2 cut(s) 291, 1354
BmrFI CCNGG 6 cut(s) 75, 209, 239, 414, 1359, 1401
BmrI ACTGGG 1 cut(s) 1097
BmsI GCATC 6 cut(s) 232, 663, 739, 757, 789, 1291
BmtI GCTAGC 1 cut(s) 441
BmuI ACTGGG 1 cut(s) 1097
BpuEI CTTGAG 2 cut(s) 463, 529
BpuMI CCSGG 2 cut(s) 209, 239
BsaI GGTCTC 1 cut(s) 1390
BsaJI CCNNGG 6 cut(s) 285, 354, 413, 924, 1113, 1357
BsaWI WCCGGW 1 cut(s) 482
BsaXI ACNNNNNCTCC 4 cut(s) 960, 990, 1387, 1417
Bsc4I CCNNNNNNNGG 4 cut(s) 486, 668, 833, 1153
Bse118I RCCGGY 1 cut(s) 377
Bse1I ACTGG 3 cut(s) 293, 1103, 1158
Bse3DI GCAATG 5 cut(s) 35, 345, 795, 849, 919
BseBI CCWGG 4 cut(s) 75, 414, 1359, 1401
BseDI CCNNGG 6 cut(s) 285, 354, 413, 924, 1113, 1357
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 4 cut(s) 486, 668, 833, 1153
BseMI GCAATG 5 cut(s) 35, 345, 795, 849, 919
BseMII CTCAG 4 cut(s) 594, 657, 865, 950
BseNI ACTGG 3 cut(s) 293, 1103, 1158
BseXI GCAGC 7 cut(s) 98, 178, 561, 584, 596, 739, 1205
Bsh1236I CGCG 2 cut(s) 858, 1385
BshFI GGCC 1 cut(s) 727
BshNI GGYRCC 1 cut(s) 289
BsiHKCI CYCGRG 1 cut(s) 160
BsiSI CCGG 5 cut(s) 209, 239, 378, 483, 1377
BslFI GGGAC 1 cut(s) 680
BslI CCNNNNNNNGG 4 cut(s) 486, 668, 833, 1153
BsmAI GTCTC 3 cut(s) 599, 803, 1390
BsmFI GGGAC 1 cut(s) 680
BsmI GAATGC 1 cut(s) 321
BsnI GGCC 1 cut(s) 727
Bso31I GGTCTC 1 cut(s) 1390
BsoBI CYCGRG 1 cut(s) 160
Bsp1286I GDGCHC 1 cut(s) 1357
Bsp143I GATC 4 cut(s) 124, 398, 1109, 1161
BspACI CCGC 6 cut(s) 375, 501, 540, 552, 587, 1231
BspANI GGCC 1 cut(s) 727
BspCNI CTCAG 4 cut(s) 595, 656, 864, 951
BspFNI CGCG 2 cut(s) 858, 1385
BspLI GGNNCC 2 cut(s) 291, 1354
BspOI GCTAGC 1 cut(s) 441
BspPI GGATC 2 cut(s) 406, 1104
BspT107I GGYRCC 1 cut(s) 289
BspTNI GGTCTC 1 cut(s) 1390
BsrDI GCAATG 5 cut(s) 35, 345, 795, 849, 919
BsrFI RCCGGY 1 cut(s) 377
BsrI ACTGG 3 cut(s) 293, 1103, 1158
BssAI RCCGGY 1 cut(s) 377
BssECI CCNNGG 6 cut(s) 285, 354, 413, 924, 1113, 1357
BssMI GATC 4 cut(s) 124, 398, 1109, 1161
BssT1I CCWWGG 4 cut(s) 285, 354, 924, 1113
Bst2UI CCWGG 4 cut(s) 75, 414, 1359, 1401
Bst4CI ACNGT 2 cut(s) 721, 742
BstAPI GCANNNNNTGC 1 cut(s) 752
BstC8I GCNNGC 6 cut(s) 91, 135, 379, 439, 778, 1473
BstDEI CTNAG 6 cut(s) 309, 603, 643, 851, 959, 1479
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 2 cut(s) 858, 1385
BstHHI GCGC 3 cut(s) 247, 279, 858
BstKTI GATC 4 cut(s) 127, 401, 1112, 1164
BstMAI GTCTC 3 cut(s) 599, 803, 1390
BstMBI GATC 4 cut(s) 124, 398, 1109, 1161
BstMWI GCNNNNNNNGC 8 cut(s) 263, 507, 546, 549, 581, 752, 1199, 1382
BstNI CCWGG 4 cut(s) 75, 414, 1359, 1401
BstSCI CCNGG 6 cut(s) 73, 207, 237, 412, 1357, 1399
BstUI CGCG 2 cut(s) 858, 1385
BstV1I GCAGC 7 cut(s) 98, 178, 561, 584, 596, 739, 1205
BstX2I RGATCY 1 cut(s) 398
BstYI RGATCY 1 cut(s) 398
BsuI GTATCC 2 cut(s) 709, 1373
BsuRI GGCC 1 cut(s) 727
BtgZI GCGATG 1 cut(s) 1044
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 2 cut(s) 389, 918
BtsIMutI CAGTG 6 cut(s) 300, 389, 573, 600, 918, 1110
Cac8I GCNNGC 6 cut(s) 91, 135, 379, 439, 778, 1473
CaiI CAGNNNCTG 2 cut(s) 752, 1153
CfoI GCGC 3 cut(s) 247, 279, 858
Cfr10I RCCGGY 1 cut(s) 377
Cfr13I GGNCC 2 cut(s) 282, 725
CsiI ACCWGGT 1 cut(s) 1399
Csp6I GTAC 1 cut(s) 820
CspCI CAANNNNNGTGG 2 cut(s) 549, 584
CviAII CATG 1 cut(s) 317
CviQI GTAC 1 cut(s) 820
DdeI CTNAG 6 cut(s) 309, 603, 643, 851, 959, 1479
DpnI GATC 4 cut(s) 126, 400, 1111, 1163
DpnII GATC 4 cut(s) 124, 398, 1109, 1161
Eco130I CCWWGG 4 cut(s) 285, 354, 924, 1113
Eco24I GRGCYC 1 cut(s) 1357
Eco31I GGTCTC 1 cut(s) 1390
Eco47I GGWCC 1 cut(s) 282
Eco57I CTGAAG 2 cut(s) 113, 1218
Eco88I CYCGRG 1 cut(s) 160
EcoRII CCWGG 4 cut(s) 73, 412, 1357, 1399
EcoT14I CCWWGG 4 cut(s) 285, 354, 924, 1113
EcoT22I ATGCAT 1 cut(s) 1078
EcoT38I GRGCYC 1 cut(s) 1357
ErhI CCWWGG 4 cut(s) 285, 354, 924, 1113
FaeI CATG 1 cut(s) 320
FalI AAGNNNNNCTT 2 cut(s) 425, 457
FaqI GGGAC 1 cut(s) 680
FatI CATG 1 cut(s) 316
FauI CCCGC 1 cut(s) 1224
FblI GTMKAC 1 cut(s) 984
Fnu4HI GCNGC 8 cut(s) 87, 167, 541, 550, 573, 585, 753, 1194
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 1 cut(s) 1357
Fsp4HI GCNGC 8 cut(s) 87, 167, 541, 550, 573, 585, 753, 1194
FspBI CTAG 5 cut(s) 227, 438, 678, 1298, 1513
GlaI GCGC 3 cut(s) 246, 278, 857
GluI GCNGC 8 cut(s) 87, 167, 541, 550, 573, 585, 753, 1194
HaeIII GGCC 1 cut(s) 727
HapII CCGG 5 cut(s) 209, 239, 378, 483, 1377
HhaI GCGC 3 cut(s) 247, 279, 858
Hin1II CATG 1 cut(s) 320
Hin6I GCGC 3 cut(s) 245, 277, 856
HinP1I GCGC 3 cut(s) 245, 277, 856
HindIII AAGCTT 1 cut(s) 13
HinfI GANTC 4 cut(s) 96, 222, 1243, 1312
HpaII CCGG 5 cut(s) 209, 239, 378, 483, 1377
HphI GGTGA 4 cut(s) 148, 242, 1294, 1321
Hpy166II GTNNAC 4 cut(s) 235, 985, 1257, 1421
Hpy188I TCNGA 3 cut(s) 197, 960, 1435
Hpy188III TCNNGA 1 cut(s) 764
Hpy8I GTNNAC 4 cut(s) 235, 985, 1257, 1421
Hpy99I CGWCG 1 cut(s) 863
HpyAV CCTTC 4 cut(s) 907, 1078, 1141, 1506
HpyCH4III ACNGT 2 cut(s) 721, 742
HpyCH4IV ACGT 2 cut(s) 822, 935
HpyCH4V TGCA 8 cut(s) 316, 350, 389, 800, 842, 1043, 1076, 1343
HpyF10VI GCNNNNNNNGC 8 cut(s) 263, 507, 546, 549, 581, 752, 1199, 1382
HpyF3I CTNAG 6 cut(s) 309, 603, 643, 851, 959, 1479
HpySE526I ACGT 2 cut(s) 822, 935
Hsp92II CATG 1 cut(s) 320
HspAI GCGC 3 cut(s) 245, 277, 856
KroI GCCGGC 1 cut(s) 377
KroNI GCCGGC 1 cut(s) 379
Kzo9I GATC 4 cut(s) 124, 398, 1109, 1161
LmnI GCTCC 1 cut(s) 1352
Lsp1109I GCAGC 7 cut(s) 98, 178, 561, 584, 596, 739, 1205
LweI GCATC 6 cut(s) 232, 663, 739, 757, 789, 1291
MabI ACCWGGT 1 cut(s) 1399
MaeI CTAG 5 cut(s) 227, 438, 678, 1298, 1513
MaeII ACGT 2 cut(s) 822, 935
MaeIII GTNAC 4 cut(s) 297, 453, 1282, 1309
MalI GATC 4 cut(s) 126, 400, 1111, 1163
MboI GATC 4 cut(s) 124, 398, 1109, 1161
MboII GAAGA 6 cut(s) 24, 46, 119, 1014, 1046, 1303
MfeI CAATTG 1 cut(s) 1344
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 1 cut(s) 1357
MluCI AATT 9 cut(s) 699, 729, 988, 996, 1003, 1235, 1277, 1344, 1446
MlyI GAGTC 3 cut(s) 90, 231, 1306
MmeI TCCRAC 1 cut(s) 467
Mph1103I ATGCAT 1 cut(s) 1078
MroNI GCCGGC 1 cut(s) 377
MseI TTAA 4 cut(s) 141, 867, 1007, 1070
MspA1I CMGCKG 3 cut(s) 552, 587, 1196
MspI CCGG 5 cut(s) 209, 239, 378, 483, 1377
MspR9I CCNGG 6 cut(s) 75, 209, 239, 414, 1359, 1401
MunI CAATTG 1 cut(s) 1344
Mva1269I GAATGC 1 cut(s) 321
MvaI CCWGG 4 cut(s) 75, 414, 1359, 1401
MvnI CGCG 2 cut(s) 858, 1385
MwoI GCNNNNNNNGC 8 cut(s) 263, 507, 546, 549, 581, 752, 1199, 1382
NaeI GCCGGC 1 cut(s) 379
NciI CCSGG 2 cut(s) 209, 239
NdeII GATC 4 cut(s) 124, 398, 1109, 1161
NgoMIV GCCGGC 1 cut(s) 377
NheI GCTAGC 1 cut(s) 437
NlaIII CATG 1 cut(s) 320
NlaIV GGNNCC 2 cut(s) 291, 1354
NmuCI GTSAC 4 cut(s) 297, 453, 1282, 1309
NsiI ATGCAT 1 cut(s) 1078
PaeR7I CTCGAG 1 cut(s) 160
PctI GAATGC 1 cut(s) 321
PdiI GCCGGC 1 cut(s) 379
PfeI GAWTC 1 cut(s) 1243
PflMI CCANNNNNTGG 1 cut(s) 1153
PkrI GCNGC 8 cut(s) 88, 168, 542, 551, 574, 586, 754, 1195
PleI GAGTC 3 cut(s) 90, 230, 1306
PpsI GAGTC 3 cut(s) 90, 230, 1306
PsiI TTATAA 1 cut(s) 639
Psp1406I AACGTT 1 cut(s) 935
Psp6I CCWGG 4 cut(s) 73, 412, 1357, 1399
PspGI CCWGG 4 cut(s) 73, 412, 1357, 1399
PspN4I GGNNCC 2 cut(s) 291, 1354
PspPI GGNCC 2 cut(s) 282, 725
PspXI VCTCGAGB 1 cut(s) 160
PstNI CAGNNNCTG 2 cut(s) 752, 1153
PsuI RGATCY 1 cut(s) 398
PvuII CAGCTG 1 cut(s) 1196
RsaI GTAC 1 cut(s) 821
RsaNI GTAC 1 cut(s) 820
SaqAI TTAA 4 cut(s) 141, 867, 1007, 1070
SatI GCNGC 8 cut(s) 87, 167, 541, 550, 573, 585, 753, 1194
Sau3AI GATC 4 cut(s) 124, 398, 1109, 1161
Sau96I GGNCC 2 cut(s) 282, 725
SchI GAGTC 3 cut(s) 90, 231, 1306
ScrFI CCNGG 6 cut(s) 75, 209, 239, 414, 1359, 1401
SduI GDGCHC 1 cut(s) 1357
SexAI ACCWGGT 1 cut(s) 1399
SfaNI GCATC 6 cut(s) 232, 663, 739, 757, 789, 1291
Sfr274I CTCGAG 1 cut(s) 160
SinI GGWCC 1 cut(s) 282
SlaI CTCGAG 1 cut(s) 160
SmlI CTYRAG 3 cut(s) 160, 442, 544
SmoI CTYRAG 3 cut(s) 160, 442, 544
SpeI ACTAGT 1 cut(s) 677
Sse9I AATT 9 cut(s) 699, 729, 988, 996, 1003, 1235, 1277, 1344, 1446
SsiI CCGC 6 cut(s) 375, 501, 540, 552, 587, 1231
SspI AATATT 1 cut(s) 179
SspMI CTAG 5 cut(s) 227, 438, 678, 1298, 1513
StyD4I CCNGG 6 cut(s) 73, 207, 237, 412, 1357, 1399
StyI CCWWGG 4 cut(s) 285, 354, 924, 1113
TaaI ACNGT 2 cut(s) 721, 742
TaiI ACGT 2 cut(s) 825, 938
TaqI TCGA 3 cut(s) 161, 682, 783
TaqII GACCGA 1 cut(s) 659
TasI AATT 9 cut(s) 699, 729, 988, 996, 1003, 1235, 1277, 1344, 1446
TauI GCSGC 1 cut(s) 543
TfiI GAWTC 1 cut(s) 1243
Tru1I TTAA 4 cut(s) 141, 867, 1007, 1070
Tru9I TTAA 4 cut(s) 141, 867, 1007, 1070
TscAI CASTG 6 cut(s) 300, 396, 573, 607, 925, 1110
TseFI GTSAC 4 cut(s) 297, 453, 1282, 1309
TseI GCWGC 7 cut(s) 86, 166, 549, 572, 584, 752, 1193
Tsp45I GTSAC 4 cut(s) 297, 453, 1282, 1309
TspDTI ATGAA 6 cut(s) 24, 54, 380, 486, 1047, 1488
TspGWI ACGGA 1 cut(s) 876
TspRI CASTG 6 cut(s) 300, 396, 573, 607, 925, 1110
Van91I CCANNNNNTGG 1 cut(s) 1153
VpaK11BI GGWCC 1 cut(s) 282
XhoI CTCGAG 1 cut(s) 160
XmiI GTMKAC 1 cut(s) 984
XspI CTAG 5 cut(s) 227, 438, 678, 1298, 1513
Zsp2I ATGCAT 1 cut(s) 1078
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.