RLG00000015336

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000427
Physical Location & Seq
Forward (+)
64746249 .. 64748677
2429 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015336

Sequence Viewer

Length: 1569 bp
ATGATCCAAATCCATTATGGCCAAAACTCCGTTGATCAGCTCAATTCTTGGGTTCTTGGTCACTGGCAGTCTGGCACTTCATTACTGCCATTATCATCCATGACTAACTATCTTCTCCTCTCGTTTTTGCTTCTAATTTTCATTCTATCATATGGCTCACAAAAATTCACAGCCAAAAGGCCCAAAATCACAATCGAAGAAGCTGCTGTTGATAATCTCCAGCTTGCTTTCAAGCTCAACACTCTGACCTCGAGGCAACTTGTTCTGTTCTACATGGACGAGATTCAAAAACTGAACAAAAACCTTAGGGCTGTGATAGAGGTGAACCCTGATGCTTTATCCCAATCTGATATAGCTGACAAACAACGCAGAGAATGCAACCACACACTGCCTATGCTTCATGGCATTCCTATTCTGCTCAAGGATACCATAGCAACCAAGGACAAACTCAACACCACAGCTGGATCATTTGCCTTGTTGGGGTCCATTGTGCCTCGAGATGCAGGTGTTGTTTCTAAGCTAAGGAGGTCTGGAGCTATCATTTTAGGGAAGGCTAGCTTGAGTGAATGGTGCCATTTCAGGTCATTCCTAGCACCTTCGGGATGGAGCGCCAGAGGTTCCCAAGGGATGAATCCTTACAATTCATCGGTGGAAGTTTGTGGATCAAGTAGTGGACCTGCAATATCATTATCAACTGACAGCTCCATTATCTGTCCGGCTAGCTTTAACTCTGTGGTGGGCATCAAACCCACTGTTGGTCTCACTAGTCGAGCAGGAGTCATACCAATCAGTCCTAGACAGGACACAGTTGGGCTGGTTTGTAGGACTGTAGCGGATGTTGTTCACGTCCTTGATATCATTGTTGGCATTGATAGGAATGATAATGCAACAAGTGAATTTCTCAAGCACAATGGCCTCAAAGGAAAGAGACTGGGGATAGTAAATATGTTATTTGATTCAATCAAGGACGATGCTTTTCTGAAACGAACTTTTGTGCAGCATTTTACCACACTGAGGAAACAAGGTGCAGTTTTGGTTGACAATCTTGAAATAGCCAATATTGAAGATGTTCTGGATTCTCATTCAAGTGGAGAATTTGATGCAATATTAGCTGAATTCAAAATAGCCTTGAATGAGTACCTAAGTGACCTAGTAAAATCTCCAGTGAAGTCCTTAAGGGAGGTGATAGAGTTCAACAAGAATCATTCAACGGAAAAGATCAATGAATATGGGCAAGATGTCCTCGAATTAGCTGAGAAGACTAATGGGATGGGACCGAAAGTGCAGGAGGCATTGTCAAATTTAAAAAGACTATCTAAACAGGGGTTCAAGAAGTTGATGACCGATCATAGCCTTGATGCCTTGGTGGCTTATGTTAACAGTGCTTCTACTATCCTCGCAATCGGTGGTTTTCCGGGCATTGTTGTGCCGGCAGGATATCATAACACTAAAAAGTATCCTTTTGGGATCTGCTTTGGGGGACTCAAGGGTTCAGAGCCAAAGCTGATTGAGATCGCCTATGCCTTTGAGCAAGCAACTAAGACAAGGAGAAAGCCTCCTCTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

523

Amino Acids

57.1

Weight (kDa)

8.98

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 87 - 457 2.7e-52 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 494
AbsI CCTCGAGG 1 cut(s) 250
Acc36I ACCTGC 2 cut(s) 494, 685
AccB1I GGYRCC 1 cut(s) 570
AciI CCGC 1 cut(s) 833
AclWI GGATC 3 cut(s) 472, 670, 1475
AcoI YGGCCR 1 cut(s) 19
AcsI RAATTY 5 cut(s) 164, 896, 1094, 1115, 1300
AfaI GTAC 1 cut(s) 1139
AfiI CCNNNNNNNGG 3 cut(s) 480, 755, 1014
AflII CTTAAG 1 cut(s) 1174
AhlI ACTAGT 1 cut(s) 764
AjiI CACGTC 1 cut(s) 847
Alw26I GTCTC 2 cut(s) 764, 922
AlwI GGATC 3 cut(s) 472, 670, 1475
Ama87I CYCGRG 2 cut(s) 250, 495
AoxI GGCC 3 cut(s) 19, 179, 913
ApeKI GCWGC 2 cut(s) 203, 997
ApoI RAATTY 5 cut(s) 164, 896, 1094, 1115, 1300
Asp700I GAANNNNTTC 1 cut(s) 1068
AspLEI GCGC 1 cut(s) 611
AspS9I GGNCC 4 cut(s) 180, 483, 674, 1274
AsuC2I CCSGG 1 cut(s) 1416
AsuHPI GGTGA 2 cut(s) 334, 1195
AsuNHI GCTAGC 2 cut(s) 554, 719
AvaI CYCGRG 2 cut(s) 250, 495
AvaII GGWCC 3 cut(s) 483, 674, 1274
AxyI CCTNAGG 1 cut(s) 305
BalI TGGCCA 1 cut(s) 21
BanI GGYRCC 1 cut(s) 570
BbsI GAAGAC 1 cut(s) 1265
BbvI GCAGC 2 cut(s) 190, 1009
BccI CCATC 2 cut(s) 597, 1264
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciVI GTATCC 2 cut(s) 418, 1467
BclI TGATCA 1 cut(s) 34
BcnI CCSGG 1 cut(s) 1416
BcoDI GTCTC 2 cut(s) 764, 922
BcuI ACTAGT 1 cut(s) 764
BfaI CTAG 7 cut(s) 555, 590, 720, 765, 795, 1151, 1562
BfmI CTRYAG 1 cut(s) 828
BfoI RGCGCY 1 cut(s) 612
BfrI CTTAAG 1 cut(s) 1174
BfuAI ACCTGC 2 cut(s) 494, 685
BfuI GTATCC 2 cut(s) 418, 1467
BglI GCCNNNNNGGC 1 cut(s) 1367
BisI GCNGC 2 cut(s) 204, 998
BlsI GCNGC 2 cut(s) 205, 999
Bme1390I CCNGG 1 cut(s) 1416
Bme18I GGWCC 3 cut(s) 483, 674, 1274
BmeT110I CYCGRG 2 cut(s) 250, 495
BmgBI CACGTC 1 cut(s) 847
BmgT120I GGNCC 4 cut(s) 180, 483, 674, 1274
BmiI GGNNCC 4 cut(s) 484, 572, 619, 1275
BmrFI CCNGG 1 cut(s) 1416
BmrI ACTGGG 1 cut(s) 941
BmsI GCATC 6 cut(s) 322, 490, 750, 961, 1090, 1348
BmtI GCTAGC 2 cut(s) 558, 723
BmuI ACTGGG 1 cut(s) 941
BpiI GAAGAC 1 cut(s) 1265
BplI GAGNNNNNCTC 1 cut(s) 1540
BpmI CTGGAG 3 cut(s) 203, 552, 1146
Bpu10I CCTNAGC 1 cut(s) 521
BpuEI CTTGAG 4 cut(s) 404, 580, 887, 1469
BpuMI CCSGG 1 cut(s) 1416
BsaBI GATNNNNATC 2 cut(s) 8, 1511
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 3 cut(s) 438, 622, 1362
BsaXI ACNNNNNCTCC 2 cut(s) 1280, 1310
Bsc4I CCNNNNNNNGG 3 cut(s) 480, 755, 1014
Bse118I RCCGGY 1 cut(s) 1429
Bse1I ACTGG 3 cut(s) 68, 936, 1163
Bse21I CCTNAGG 1 cut(s) 305
Bse8I GATNNNNATC 2 cut(s) 8, 1511
BseDI CCNNGG 3 cut(s) 438, 622, 1362
BseGI GGATG 5 cut(s) 95, 608, 633, 841, 1275
BseJI GATNNNNATC 2 cut(s) 8, 1511
BseLI CCNNNNNNNGG 3 cut(s) 480, 755, 1014
BseMII CTCAG 2 cut(s) 1004, 1245
BseNI ACTGG 3 cut(s) 68, 936, 1163
BseRI GAGGAG 2 cut(s) 107, 1548
BseXI GCAGC 2 cut(s) 190, 1009
BsgI GTGCAG 3 cut(s) 1016, 1047, 1304
BshFI GGCC 3 cut(s) 21, 181, 915
BshNI GGYRCC 1 cut(s) 570
BsiHKCI CYCGRG 2 cut(s) 250, 495
BsiSI CCGG 3 cut(s) 716, 1415, 1430
BslFI GGGAC 2 cut(s) 1287, 1494
BslI CCNNNNNNNGG 3 cut(s) 480, 755, 1014
BsmAI GTCTC 2 cut(s) 764, 922
BsmFI GGGAC 2 cut(s) 1287, 1494
BsmI GAATGC 2 cut(s) 380, 405
BsnI GGCC 3 cut(s) 21, 181, 915
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 2 cut(s) 250, 495
Bsp143I GATC 8 cut(s) 3, 34, 464, 662, 1218, 1345, 1467, 1512
BspACI CCGC 1 cut(s) 833
BspANI GGCC 3 cut(s) 21, 181, 915
BspCNI CTCAG 2 cut(s) 1005, 1246
BspLI GGNNCC 4 cut(s) 484, 572, 619, 1275
BspMI ACCTGC 2 cut(s) 494, 685
BspOI GCTAGC 2 cut(s) 558, 723
BspPI GGATC 3 cut(s) 472, 670, 1475
BspT107I GGYRCC 1 cut(s) 570
BspTI CTTAAG 1 cut(s) 1174
BspTNI GGTCTC 1 cut(s) 764
BsrFI RCCGGY 1 cut(s) 1429
BsrI ACTGG 3 cut(s) 68, 936, 1163
BssAI RCCGGY 1 cut(s) 1429
BssECI CCNNGG 3 cut(s) 438, 622, 1362
BssMI GATC 8 cut(s) 3, 34, 464, 662, 1218, 1345, 1467, 1512
BssT1I CCWWGG 3 cut(s) 438, 622, 1362
Bst4CI ACNGT 4 cut(s) 754, 808, 829, 1382
BstAFI CTTAAG 1 cut(s) 1174
BstAPI GCANNNNNTGC 1 cut(s) 375
BstC8I GCNNGC 5 cut(s) 225, 556, 721, 1431, 1533
BstDEI CTNAG 7 cut(s) 305, 516, 521, 1013, 1142, 1254, 1539
BstF5I GGATG 5 cut(s) 95, 608, 633, 841, 1275
BstH2I RGCGCY 1 cut(s) 612
BstHHI GCGC 1 cut(s) 611
BstKTI GATC 8 cut(s) 6, 37, 467, 665, 1221, 1348, 1470, 1515
BstMAI GTCTC 2 cut(s) 764, 922
BstMBI GATC 8 cut(s) 3, 34, 464, 662, 1218, 1345, 1467, 1512
BstMWI GCNNNNNNNGC 3 cut(s) 375, 1109, 1367
BstSCI CCNGG 1 cut(s) 1414
BstSFI CTRYAG 1 cut(s) 828
BstV1I GCAGC 2 cut(s) 190, 1009
BstV2I GAAGAC 1 cut(s) 1265
BstX2I RGATCY 1 cut(s) 1467
BstYI RGATCY 1 cut(s) 1467
Bsu36I CCTNAGG 1 cut(s) 305
BsuI GTATCC 2 cut(s) 418, 1467
BsuRI GGCC 3 cut(s) 21, 181, 915
BtrI CACGTC 1 cut(s) 847
BtsCI GGATG 5 cut(s) 95, 608, 633, 841, 1275
BtsI GCAGTG 1 cut(s) 386
BtsIMutI CAGTG 6 cut(s) 61, 386, 750, 1010, 1170, 1387
BveI ACCTGC 2 cut(s) 494, 685
Cac8I GCNNGC 5 cut(s) 225, 556, 721, 1431, 1533
CfoI GCGC 1 cut(s) 611
Cfr10I RCCGGY 1 cut(s) 1429
Cfr13I GGNCC 4 cut(s) 180, 483, 674, 1274
Csp6I GTAC 1 cut(s) 1138
CviAII CATG 3 cut(s) 100, 274, 401
CviQI GTAC 1 cut(s) 1138
DdeI CTNAG 7 cut(s) 305, 516, 521, 1013, 1142, 1254, 1539
DpnI GATC 8 cut(s) 5, 36, 466, 664, 1220, 1347, 1469, 1514
DpnII GATC 8 cut(s) 3, 34, 464, 662, 1218, 1345, 1467, 1512
DraI TTTAAA 1 cut(s) 1305
EaeI YGGCCR 1 cut(s) 19
Eco130I CCWWGG 3 cut(s) 438, 622, 1362
Eco31I GGTCTC 1 cut(s) 764
Eco32I GATATC 2 cut(s) 856, 1439
Eco47I GGWCC 3 cut(s) 483, 674, 1274
Eco81I CCTNAGG 1 cut(s) 305
Eco88I CYCGRG 2 cut(s) 250, 495
EcoRI GAATTC 1 cut(s) 1115
EcoRV GATATC 2 cut(s) 856, 1439
EcoT14I CCWWGG 3 cut(s) 438, 622, 1362
ErhI CCWWGG 3 cut(s) 438, 622, 1362
FaeI CATG 3 cut(s) 103, 277, 404
FalI AAGNNNNNCTT 2 cut(s) 542, 574
FaqI GGGAC 2 cut(s) 1287, 1494
FatI CATG 3 cut(s) 99, 273, 400
FauNDI CATATG 1 cut(s) 151
FbaI TGATCA 1 cut(s) 34
Fnu4HI GCNGC 2 cut(s) 204, 998
FokI GGATG 5 cut(s) 82, 615, 640, 848, 1282
Fsp4HI GCNGC 2 cut(s) 204, 998
FspBI CTAG 7 cut(s) 555, 590, 720, 765, 795, 1151, 1562
GlaI GCGC 1 cut(s) 610
GluI GCNGC 2 cut(s) 204, 998
GsuI CTGGAG 3 cut(s) 203, 552, 1146
HaeII RGCGCY 1 cut(s) 612
HaeIII GGCC 3 cut(s) 21, 181, 915
HapII CCGG 3 cut(s) 716, 1415, 1430
HhaI GCGC 1 cut(s) 611
Hin1II CATG 3 cut(s) 103, 277, 404
Hin6I GCGC 1 cut(s) 609
HinP1I GCGC 1 cut(s) 609
HincII GTYRAC 2 cut(s) 1039, 1378
HindII GTYRAC 2 cut(s) 1039, 1378
HinfI GANTC 7 cut(s) 283, 631, 777, 956, 1076, 1201, 1482
HpaI GTTAAC 1 cut(s) 1378
HpaII CCGG 3 cut(s) 716, 1415, 1430
HphI GGTGA 2 cut(s) 334, 1195
Hpy166II GTNNAC 5 cut(s) 325, 674, 844, 1039, 1378
Hpy188I TCNGA 4 cut(s) 246, 349, 981, 1495
Hpy188III TCNNGA 6 cut(s) 497, 531, 600, 1046, 1073, 1330
Hpy8I GTNNAC 5 cut(s) 325, 674, 844, 1039, 1378
HpyAV CCTTC 2 cut(s) 544, 606
HpyCH4III ACNGT 4 cut(s) 754, 808, 829, 1382
HpyCH4IV ACGT 1 cut(s) 846
HpyCH4V TGCA 8 cut(s) 378, 503, 680, 887, 997, 1028, 1103, 1285
HpyF10VI GCNNNNNNNGC 3 cut(s) 375, 1109, 1367
HpyF3I CTNAG 7 cut(s) 305, 516, 521, 1013, 1142, 1254, 1539
HpySE526I ACGT 1 cut(s) 846
Hsp92II CATG 3 cut(s) 103, 277, 404
HspAI GCGC 1 cut(s) 609
KroI GCCGGC 1 cut(s) 1429
KroNI GCCGGC 1 cut(s) 1431
Ksp22I TGATCA 1 cut(s) 34
KspAI GTTAAC 1 cut(s) 1378
Kzo9I GATC 8 cut(s) 3, 34, 464, 662, 1218, 1345, 1467, 1512
LmnI GCTCC 3 cut(s) 533, 606, 707
Lsp1109I GCAGC 2 cut(s) 190, 1009
LweI GCATC 6 cut(s) 322, 490, 750, 961, 1090, 1348
MaeI CTAG 7 cut(s) 555, 590, 720, 765, 795, 1151, 1562
MaeII ACGT 1 cut(s) 846
MaeIII GTNAC 2 cut(s) 59, 1145
MalI GATC 8 cut(s) 5, 36, 466, 664, 1220, 1347, 1469, 1514
MboI GATC 8 cut(s) 3, 34, 464, 662, 1218, 1345, 1467, 1512
MboII GAAGA 4 cut(s) 104, 209, 1076, 1270
MflI RGATCY 1 cut(s) 1467
MlsI TGGCCA 1 cut(s) 21
MluCI AATT 9 cut(s) 43, 135, 164, 640, 896, 1094, 1115, 1247, 1300
MluNI TGGCCA 1 cut(s) 21
MlyI GAGTC 2 cut(s) 786, 1476
Mox20I TGGCCA 1 cut(s) 21
MroNI GCCGGC 1 cut(s) 1429
MroXI GAANNNNTTC 1 cut(s) 1068
MscI TGGCCA 1 cut(s) 21
MseI TTAA 4 cut(s) 726, 1175, 1304, 1377
MslI CAYNNNNRTG 2 cut(s) 1086, 1424
Msp20I TGGCCA 1 cut(s) 21
MspA1I CMGCKG 1 cut(s) 461
MspCI CTTAAG 1 cut(s) 1174
MspI CCGG 3 cut(s) 716, 1415, 1430
MspR9I CCNGG 1 cut(s) 1416
Mva1269I GAATGC 2 cut(s) 380, 405
MwoI GCNNNNNNNGC 3 cut(s) 375, 1109, 1367
NaeI GCCGGC 1 cut(s) 1431
NciI CCSGG 1 cut(s) 1416
NdeI CATATG 1 cut(s) 151
NdeII GATC 8 cut(s) 3, 34, 464, 662, 1218, 1345, 1467, 1512
NgoMIV GCCGGC 1 cut(s) 1429
NheI GCTAGC 2 cut(s) 554, 719
NlaIII CATG 3 cut(s) 103, 277, 404
NlaIV GGNNCC 4 cut(s) 484, 572, 619, 1275
NmuCI GTSAC 2 cut(s) 59, 1145
PaeR7I CTCGAG 2 cut(s) 250, 495
PaqCI CACCTGC 1 cut(s) 494
PctI GAATGC 2 cut(s) 380, 405
PdiI GCCGGC 1 cut(s) 1431
PdmI GAANNNNTTC 1 cut(s) 1068
PfeI GAWTC 5 cut(s) 283, 631, 956, 1076, 1201
PkrI GCNGC 2 cut(s) 205, 999
PleI GAGTC 2 cut(s) 785, 1476
PpsI GAGTC 2 cut(s) 785, 1476
PspN4I GGNNCC 4 cut(s) 484, 572, 619, 1275
PspPI GGNCC 4 cut(s) 180, 483, 674, 1274
PspXI VCTCGAGB 1 cut(s) 250
PsuI RGATCY 1 cut(s) 1467
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 1 cut(s) 1139
RsaNI GTAC 1 cut(s) 1138
RseI CAYNNNNRTG 2 cut(s) 1086, 1424
SaqAI TTAA 4 cut(s) 726, 1175, 1304, 1377
SatI GCNGC 2 cut(s) 204, 998
Sau3AI GATC 8 cut(s) 3, 34, 464, 662, 1218, 1345, 1467, 1512
Sau96I GGNCC 4 cut(s) 180, 483, 674, 1274
SchI GAGTC 2 cut(s) 786, 1476
ScrFI CCNGG 1 cut(s) 1416
SfaNI GCATC 6 cut(s) 322, 490, 750, 961, 1090, 1348
SfcI CTRYAG 1 cut(s) 828
Sfr274I CTCGAG 2 cut(s) 250, 495
SinI GGWCC 3 cut(s) 483, 674, 1274
SlaI CTCGAG 2 cut(s) 250, 495
SmiMI CAYNNNNRTG 2 cut(s) 1086, 1424
SmlI CTYRAG 7 cut(s) 250, 419, 495, 559, 902, 1174, 1484
SmoI CTYRAG 7 cut(s) 250, 419, 495, 559, 902, 1174, 1484
SpeI ACTAGT 1 cut(s) 764
Sse9I AATT 9 cut(s) 43, 135, 164, 640, 896, 1094, 1115, 1247, 1300
SsiI CCGC 1 cut(s) 833
SspI AATATT 2 cut(s) 1060, 1107
SspMI CTAG 7 cut(s) 555, 590, 720, 765, 795, 1151, 1562
StyD4I CCNGG 1 cut(s) 1414
StyI CCWWGG 3 cut(s) 438, 622, 1362
TaaI ACNGT 4 cut(s) 754, 808, 829, 1382
TaiI ACGT 1 cut(s) 849
TaqI TCGA 5 cut(s) 195, 251, 496, 769, 1245
TaqII GACCGA 2 cut(s) 1291, 1358
TasI AATT 9 cut(s) 43, 135, 164, 640, 896, 1094, 1115, 1247, 1300
TfiI GAWTC 5 cut(s) 283, 631, 956, 1076, 1201
Tru1I TTAA 4 cut(s) 726, 1175, 1304, 1377
Tru9I TTAA 4 cut(s) 726, 1175, 1304, 1377
TscAI CASTG 6 cut(s) 68, 393, 757, 1017, 1170, 1387
TseFI GTSAC 2 cut(s) 59, 1145
TseI GCWGC 2 cut(s) 203, 997
Tsp45I GTSAC 2 cut(s) 59, 1145
TspDTI ATGAA 6 cut(s) 69, 130, 389, 633, 644, 1239
TspGWI ACGGA 2 cut(s) 19, 1226
TspRI CASTG 6 cut(s) 68, 393, 757, 1017, 1170, 1387
Vha464I CTTAAG 1 cut(s) 1174
VpaK11BI GGWCC 3 cut(s) 483, 674, 1274
XapI RAATTY 5 cut(s) 164, 896, 1094, 1115, 1300
XcmI CCANNNNNNNNNTGG 1 cut(s) 14
XhoI CTCGAG 2 cut(s) 250, 495
XmnI GAANNNNTTC 1 cut(s) 1068
XspI CTAG 7 cut(s) 555, 590, 720, 765, 795, 1151, 1562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.