Rh6DG020100

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000427
Physical Location & Seq
Reverse (-)
1783598 .. 1785253
1656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG020100.1

Sequence Viewer

Length: 852 bp
ATGGCAACCAATTCGTGTTCTCTCTTCTGGGTTCTGCTTCTAATTCTTCAAAGCACACTATCAAACGGGTCTGAATCCATTACACCCAGAAGCCTAATGGAAGAAGCCACAGTTGATGATCTCCAACTCGCTTTCAAGCAAAAGCTTCTGACTTCAAGGCAAGTCGTTCAGTTCTACCTGAACCAAATCCACAAACTCAATCAAGACCTCAGAGGAGTCTTAGAGATAAACCCAGATGCTTTATCCCAAGCTGACATAGCAGACAAAGAGCGCAGTGAATATGAATCCAGTGCCCAAACACTGCCTAAGCTTCATGGGATTCCTGTTCTGCTCAAGGATAATATAGCAACCAAGGACAAGCTTAACACCACTGCTGGGTCTTATGCTCTTTTGGGTTCCGTAGTGCCTAGAGATGCTGGGGTTGTCTCAAAGTTGAGGAGCTCTGGGGCTATCATTTTGGGGAAGGCTACTTTGAGTGAATGGGCCTATATCAGAACATCTGAAGCACCCTATGGTTGGAGTGCCAGAGGTGGTCAAGGAGTGAATCCTTATAATTCATCACTGGAAGTTTGTGGATCAAGTAGTGGATCCGCAATATCAGTATCTGCAAATATGGTATCAGTGTCTCTAGGAACAGAGACCGATAGCTCCATCTTATGTCCAGCGAGTTTTAACTCGGTAGTGGGCTTCAAACCAACGGTTGGTCTCACTAGTCGAGCAGGAGTCATCCCAGTCAGTCCAAGACAGGACACAATTGGGCCAATTTGTAGGACTGTAGCGGATGCTGTTCATGTCCTTGATACCATTGTTGGCATTGATAGCAATGACAACGCAACAAATGAAGTATCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

283

Amino Acids

30.0

Weight (kDa)

5.26

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 55 - 280 1.6e-65 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 552
AccB7I CCANNNNNTGG 1 cut(s) 701
AciI CCGC 2 cut(s) 591, 779
AclWI GGATC 3 cut(s) 582, 583, 595
AcuI CTGAAG 1 cut(s) 522
AfiI CCNNNNNNNGG 3 cut(s) 375, 516, 701
AgsI TTSAA 4 cut(s) 50, 136, 156, 691
AhlI ACTAGT 1 cut(s) 710
AluBI AGCT 6 cut(s) 145, 251, 310, 361, 441, 648
AluI AGCT 6 cut(s) 145, 251, 310, 361, 441, 648
Alw21I GWGCWC 1 cut(s) 443
Alw26I GTCTC 4 cut(s) 430, 630, 632, 710
AlwI GGATC 3 cut(s) 582, 583, 595
AlwNI CAGNNNCTG 1 cut(s) 605
AoxI GGCC 2 cut(s) 483, 758
AspLEI GCGC 1 cut(s) 273
AspS9I GGNCC 2 cut(s) 483, 758
BaeGI GKGCMC 1 cut(s) 295
BaeI ACNNNNGTAYC 2 cut(s) 792, 825
BamHI GGATCC 1 cut(s) 587
BanII GRGCYC 1 cut(s) 443
Bbv12I GWGCWC 1 cut(s) 443
BccI CCATC 1 cut(s) 659
BcgI CGANNNNNNTGC 1 cut(s) 28
BcoDI GTCTC 4 cut(s) 430, 630, 632, 710
BcuI ACTAGT 1 cut(s) 710
BfaI CTAG 4 cut(s) 408, 629, 711, 850
BfmI CTRYAG 1 cut(s) 774
BmgT120I GGNCC 2 cut(s) 483, 758
BmiI GGNNCC 2 cut(s) 397, 589
BmrI ACTGGG 1 cut(s) 725
BmsI GCATC 3 cut(s) 226, 403, 772
BmuI ACTGGG 1 cut(s) 725
Bpu10I CCTNAGC 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 317
BsaI GGTCTC 2 cut(s) 632, 710
BsaJI CCNNGG 1 cut(s) 351
Bsc4I CCNNNNNNNGG 3 cut(s) 375, 516, 701
Bse1I ACTGG 3 cut(s) 288, 567, 731
Bse3DI GCAATG 1 cut(s) 829
BseDI CCNNGG 1 cut(s) 351
BseGI GGATG 2 cut(s) 726, 787
BseLI CCNNNNNNNGG 3 cut(s) 375, 516, 701
BseMI GCAATG 1 cut(s) 829
BseMII CTCAG 1 cut(s) 223
BseNI ACTGG 3 cut(s) 288, 567, 731
BseRI GAGGAG 2 cut(s) 228, 451
BseSI GKGCMC 1 cut(s) 295
BseYI CCCAGC 2 cut(s) 374, 416
BshFI GGCC 2 cut(s) 485, 760
BsiHKAI GWGCWC 1 cut(s) 443
BslI CCNNNNNNNGG 3 cut(s) 375, 516, 701
BsmAI GTCTC 4 cut(s) 430, 630, 632, 710
BsnI GGCC 2 cut(s) 485, 760
Bso31I GGTCTC 2 cut(s) 632, 710
Bsp1286I GDGCHC 2 cut(s) 295, 443
Bsp143I GATC 3 cut(s) 118, 575, 587
BspACI CCGC 2 cut(s) 591, 779
BspANI GGCC 2 cut(s) 485, 760
BspCNI CTCAG 1 cut(s) 222
BspLI GGNNCC 2 cut(s) 397, 589
BspPI GGATC 3 cut(s) 582, 583, 595
BspTNI GGTCTC 2 cut(s) 632, 710
BsrDI GCAATG 1 cut(s) 829
BsrI ACTGG 3 cut(s) 288, 567, 731
BssECI CCNNGG 1 cut(s) 351
BssMI GATC 3 cut(s) 118, 575, 587
BssT1I CCWWGG 1 cut(s) 351
Bst4CI ACNGT 3 cut(s) 112, 700, 775
Bst6I CTCTTC 1 cut(s) 29
BstDEI CTNAG 3 cut(s) 209, 220, 306
BstF5I GGATG 2 cut(s) 726, 787
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 3 cut(s) 121, 578, 590
BstMAI GTCTC 4 cut(s) 430, 630, 632, 710
BstMBI GATC 3 cut(s) 118, 575, 587
BstMWI GCNNNNNNNGC 2 cut(s) 257, 819
BstSFI CTRYAG 1 cut(s) 774
BstSLI GKGCMC 1 cut(s) 295
BstX2I RGATCY 1 cut(s) 587
BstYI RGATCY 1 cut(s) 587
BsuRI GGCC 2 cut(s) 485, 760
BtsCI GGATG 2 cut(s) 726, 787
BtsI GCAGTG 3 cut(s) 280, 299, 369
BtsIMutI CAGTG 6 cut(s) 280, 295, 299, 369, 560, 627
CaiI CAGNNNCTG 1 cut(s) 605
CfoI GCGC 1 cut(s) 273
Cfr13I GGNCC 2 cut(s) 483, 758
CviAII CATG 2 cut(s) 314, 791
DdeI CTNAG 3 cut(s) 209, 220, 306
DpnI GATC 3 cut(s) 120, 577, 589
DpnII GATC 3 cut(s) 118, 575, 587
Eam1104I CTCTTC 1 cut(s) 29
EarI CTCTTC 1 cut(s) 29
Ecl136II GAGCTC 1 cut(s) 441
Eco130I CCWWGG 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 443
Eco31I GGTCTC 2 cut(s) 632, 710
Eco53kI GAGCTC 1 cut(s) 441
Eco57I CTGAAG 1 cut(s) 522
EcoICRI GAGCTC 1 cut(s) 441
EcoT14I CCWWGG 1 cut(s) 351
EcoT38I GRGCYC 1 cut(s) 443
ErhI CCWWGG 1 cut(s) 351
FaeI CATG 2 cut(s) 317, 794
FatI CATG 2 cut(s) 313, 790
FokI GGATG 2 cut(s) 713, 794
FriOI GRGCYC 1 cut(s) 443
FspBI CTAG 4 cut(s) 408, 629, 711, 850
GlaI GCGC 1 cut(s) 272
GsaI CCCAGC 2 cut(s) 378, 420
HaeIII GGCC 2 cut(s) 485, 760
HhaI GCGC 1 cut(s) 273
Hin1II CATG 2 cut(s) 317, 794
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HindIII AAGCTT 3 cut(s) 143, 308, 359
HinfI GANTC 6 cut(s) 74, 216, 284, 319, 544, 723
Hpy188I TCNGA 5 cut(s) 73, 150, 212, 494, 502
Hpy188III TCNNGA 1 cut(s) 203
HpyAV CCTTC 1 cut(s) 457
HpyCH4III ACNGT 3 cut(s) 112, 700, 775
HpyCH4V TGCA 1 cut(s) 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 819
HpyF3I CTNAG 3 cut(s) 209, 220, 306
Hsp92II CATG 2 cut(s) 317, 794
HspAI GCGC 1 cut(s) 271
Kzo9I GATC 3 cut(s) 118, 575, 587
LmnI GCTCC 2 cut(s) 438, 653
LweI GCATC 3 cut(s) 226, 403, 772
MaeI CTAG 4 cut(s) 408, 629, 711, 850
MalI GATC 3 cut(s) 120, 577, 589
MboI GATC 3 cut(s) 118, 575, 587
MboII GAAGA 3 cut(s) 16, 38, 113
MfeI CAATTG 1 cut(s) 753
MflI RGATCY 1 cut(s) 587
MhlI GDGCHC 2 cut(s) 295, 443
MluCI AATT 5 cut(s) 10, 42, 553, 753, 762
MlyI GAGTC 2 cut(s) 225, 732
MmeI TCCRAC 2 cut(s) 148, 497
MnlI CCTC 4 cut(s) 206, 218, 429, 521
MseI TTAA 2 cut(s) 363, 672
MunI CAATTG 1 cut(s) 753
MwoI GCNNNNNNNGC 2 cut(s) 257, 819
NdeII GATC 3 cut(s) 118, 575, 587
NlaIII CATG 2 cut(s) 317, 794
NlaIV GGNNCC 2 cut(s) 397, 589
PfeI GAWTC 4 cut(s) 74, 284, 319, 544
PflMI CCANNNNNTGG 1 cut(s) 701
PleI GAGTC 2 cut(s) 224, 731
PpsI GAGTC 2 cut(s) 224, 731
PsiI TTATAA 1 cut(s) 552
Psp124BI GAGCTC 1 cut(s) 443
PspFI CCCAGC 2 cut(s) 374, 416
PspN4I GGNNCC 2 cut(s) 397, 589
PspPI GGNCC 2 cut(s) 483, 758
PstNI CAGNNNCTG 1 cut(s) 605
PsuI RGATCY 1 cut(s) 587
SacI GAGCTC 1 cut(s) 443
SaqAI TTAA 2 cut(s) 363, 672
Sau3AI GATC 3 cut(s) 118, 575, 587
Sau96I GGNCC 2 cut(s) 483, 758
SchI GAGTC 2 cut(s) 225, 732
SduI GDGCHC 2 cut(s) 295, 443
SetI ASST 9 cut(s) 147, 180, 210, 253, 312, 363, 443, 532, 650
SfaNI GCATC 3 cut(s) 226, 403, 772
SfcI CTRYAG 1 cut(s) 774
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
SpeI ACTAGT 1 cut(s) 710
Sse9I AATT 5 cut(s) 10, 42, 553, 753, 762
SsiI CCGC 2 cut(s) 591, 779
SspMI CTAG 4 cut(s) 408, 629, 711, 850
SstI GAGCTC 1 cut(s) 443
StyI CCWWGG 1 cut(s) 351
TaaI ACNGT 3 cut(s) 112, 700, 775
TaqI TCGA 1 cut(s) 715
TaqII GACCGA 1 cut(s) 656
TasI AATT 5 cut(s) 10, 42, 553, 753, 762
TfiI GAWTC 4 cut(s) 74, 284, 319, 544
Tru1I TTAA 2 cut(s) 363, 672
Tru9I TTAA 2 cut(s) 363, 672
TscAI CASTG 6 cut(s) 280, 295, 306, 376, 567, 627
TspDTI ATGAA 4 cut(s) 297, 302, 546, 779
TspGWI ACGGA 1 cut(s) 388
TspRI CASTG 6 cut(s) 280, 295, 306, 376, 567, 627
Van91I CCANNNNNTGG 1 cut(s) 701
XcmI CCANNNNNNNNNTGG 1 cut(s) 94
XspI CTAG 4 cut(s) 408, 629, 711, 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.