RchiOBHm_Chr6g0244701

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000427
Physical Location & Seq
Reverse (-)
716081 .. 718064
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21931

Sequence Viewer

Length: 663 bp
ATGGCTTGGCCAATGTGTAGGACAGTAGCAGATGCTGCTTATGTTCTTGATGCCATAGCAGGCATCTACCACAATGACATTGCAACGATTGAGACATCGAAGTACATTCCCAAAGGTGGTTATGCACAGTTTCTCAGGCCACTACAAGAGAAAATGAAAGGAGGTGCTGTTTTGGTAGACAATTTGAAAATTGCTAATTTGACTGAAATCTGTTCCTCAAACGATGAATATAATGCATTGTATATTGAGTTCAAAATATCCTTAAATGCATACTTGAAGGAGTTGGTGGCTTCCCCAGTGCGATCCTTGGCAGATGCTATAGCCTTTAACAACAAACATCAGAAACTTGAAACGATCAAGGAGTATGGGCAAGACGTATTTTTAGCTGCTGAAGCAACAAATGGGTTTGGAAAAACAGAGAAGCCACCATTGTTGAATCTTGCAAGGTGGACAAGATATGGTCTTGTGAAATTAATGACAGAGAAGAAGCTAGATGCTGTGGTGACTCCTAAATCAGATTTTCGAATTGTACTTGCAATTGGGGGAGCCCCAGGTCTAACAGTACGTTCCAGCTGGATACAAAAACAATCACTACACCAAAAATCTTATCAGACAACGGCAGAAAACCGATGTGGGATTTGGAGTCCCACCGTTGTAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

220

Amino Acids

24.43

Weight (kDa)

8.84

Isoelectric Point (pI)

33.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 177
AclWI GGATC 1 cut(s) 297
AcoI YGGCCR 1 cut(s) 8
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 3 cut(s) 104, 531, 564
AfiI CCNNNNNNNGG 1 cut(s) 116
AgsI TTSAA 5 cut(s) 187, 253, 277, 350, 436
AjnI CCWGG 1 cut(s) 550
AluBI AGCT 3 cut(s) 386, 490, 573
AluI AGCT 3 cut(s) 386, 490, 573
Alw26I GTCTC 1 cut(s) 86
AlwI GGATC 1 cut(s) 297
AlwNI CAGNNNCTG 1 cut(s) 35
AoxI GGCC 2 cut(s) 8, 137
ApeKI GCWGC 2 cut(s) 35, 386
AseI ATTAAT 1 cut(s) 473
AsuHPI GGTGA 1 cut(s) 514
AsuII TTCGAA 1 cut(s) 523
BalI TGGCCA 1 cut(s) 10
BanII GRGCYC 1 cut(s) 550
BbvI GCAGC 2 cut(s) 22, 373
BceAI ACGGC 1 cut(s) 633
BciT130I CCWGG 1 cut(s) 552
BciVI GTATCC 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 86
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 1 cut(s) 318
BfuI GTATCC 1 cut(s) 570
BisI GCNGC 2 cut(s) 36, 387
BlsI GCNGC 2 cut(s) 37, 388
Bme1390I CCNGG 1 cut(s) 552
BmiI GGNNCC 1 cut(s) 547
BmrFI CCNGG 1 cut(s) 552
BmrI ACTGGG 1 cut(s) 290
BmsI GCATC 5 cut(s) 22, 40, 72, 304, 484
BmuI ACTGGG 1 cut(s) 290
Bpu14I TTCGAA 1 cut(s) 523
BsaJI CCNNGG 2 cut(s) 306, 550
BsaXI ACNNNNNCTCC 2 cut(s) 153, 183
Bsc4I CCNNNNNNNGG 1 cut(s) 116
Bse1I ACTGG 1 cut(s) 296
Bse3DI GCAATG 1 cut(s) 78
BseBI CCWGG 1 cut(s) 552
BseDI CCNNGG 2 cut(s) 306, 550
BseLI CCNNNNNNNGG 1 cut(s) 116
BseMI GCAATG 1 cut(s) 78
BseMII CTCAG 1 cut(s) 148
BseNI ACTGG 1 cut(s) 296
BseXI GCAGC 2 cut(s) 22, 373
BshFI GGCC 2 cut(s) 10, 139
BslFI GGGAC 1 cut(s) 630
BslI CCNNNNNNNGG 1 cut(s) 116
BsmAI GTCTC 1 cut(s) 86
BsmFI GGGAC 1 cut(s) 630
BsnI GGCC 2 cut(s) 10, 139
Bsp119I TTCGAA 1 cut(s) 523
Bsp1286I GDGCHC 1 cut(s) 550
Bsp143I GATC 2 cut(s) 302, 354
BspANI GGCC 2 cut(s) 10, 139
BspCNI CTCAG 1 cut(s) 147
BspLI GGNNCC 1 cut(s) 547
BspPI GGATC 1 cut(s) 297
BspT104I TTCGAA 1 cut(s) 523
BsrDI GCAATG 1 cut(s) 78
BsrI ACTGG 1 cut(s) 296
BssECI CCNNGG 2 cut(s) 306, 550
BssMI GATC 2 cut(s) 302, 354
BssT1I CCWWGG 1 cut(s) 306
Bst2UI CCWGG 1 cut(s) 552
Bst4CI ACNGT 4 cut(s) 25, 129, 562, 652
BstAPI GCANNNNNTGC 1 cut(s) 35
BstBI TTCGAA 1 cut(s) 523
BstC8I GCNNGC 1 cut(s) 61
BstDEI CTNAG 1 cut(s) 134
BstKTI GATC 2 cut(s) 305, 357
BstMAI GTCTC 1 cut(s) 86
BstMBI GATC 2 cut(s) 302, 354
BstMWI GCNNNNNNNGC 2 cut(s) 35, 392
BstNI CCWGG 1 cut(s) 552
BstSCI CCNGG 1 cut(s) 550
BstSFI CTRYAG 1 cut(s) 318
BstV1I GCAGC 2 cut(s) 22, 373
BsuI GTATCC 1 cut(s) 570
BsuRI GGCC 2 cut(s) 10, 139
BtsIMutI CAGTG 1 cut(s) 303
Cac8I GCNNGC 1 cut(s) 61
CaiI CAGNNNCTG 1 cut(s) 35
Csp6I GTAC 3 cut(s) 103, 530, 563
CspCI CAANNNNNGTGG 2 cut(s) 414, 449
CviQI GTAC 3 cut(s) 103, 530, 563
DdeI CTNAG 1 cut(s) 134
DpnI GATC 2 cut(s) 304, 356
DpnII GATC 2 cut(s) 302, 354
EaeI YGGCCR 1 cut(s) 8
Eco130I CCWWGG 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 550
Eco57I CTGAAG 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 550
EcoT14I CCWWGG 1 cut(s) 306
EcoT22I ATGCAT 2 cut(s) 238, 271
EcoT38I GRGCYC 1 cut(s) 550
ErhI CCWWGG 1 cut(s) 306
FaiI YATR 9 cut(s) 42, 56, 123, 231, 243, 271, 320, 366, 459
FaqI GGGAC 1 cut(s) 630
FblI GTMKAC 1 cut(s) 177
Fnu4HI GCNGC 2 cut(s) 36, 387
FriOI GRGCYC 1 cut(s) 550
Fsp4HI GCNGC 2 cut(s) 36, 387
FspBI CTAG 1 cut(s) 491
GluI GCNGC 2 cut(s) 36, 387
HaeIII GGCC 2 cut(s) 10, 139
HinfI GANTC 3 cut(s) 436, 505, 643
HphI GGTGA 1 cut(s) 514
Hpy166II GTNNAC 2 cut(s) 178, 450
Hpy188I TCNGA 3 cut(s) 342, 517, 612
Hpy188III TCNNGA 1 cut(s) 47
Hpy8I GTNNAC 2 cut(s) 178, 450
HpyAV CCTTC 1 cut(s) 271
HpyCH4III ACNGT 4 cut(s) 25, 129, 562, 652
HpyCH4IV ACGT 2 cut(s) 375, 565
HpyCH4V TGCA 6 cut(s) 83, 125, 236, 269, 443, 536
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 392
HpyF3I CTNAG 1 cut(s) 134
HpySE526I ACGT 2 cut(s) 375, 565
Kzo9I GATC 2 cut(s) 302, 354
LmnI GCTCC 1 cut(s) 545
LpnPI CCDG 7 cut(s) 45, 121, 309, 537, 559, 564, 583
Lsp1109I GCAGC 2 cut(s) 22, 373
LweI GCATC 5 cut(s) 22, 40, 72, 304, 484
MaeI CTAG 1 cut(s) 491
MaeII ACGT 2 cut(s) 375, 565
MaeIII GTNAC 1 cut(s) 502
MalI GATC 2 cut(s) 304, 356
MboI GATC 2 cut(s) 302, 354
MboII GAAGA 1 cut(s) 496
MfeI CAATTG 1 cut(s) 537
MhlI GDGCHC 1 cut(s) 550
MlsI TGGCCA 1 cut(s) 10
MluCI AATT 6 cut(s) 181, 189, 196, 470, 525, 537
MluNI TGGCCA 1 cut(s) 10
MlyI GAGTC 2 cut(s) 499, 652
MnlI CCTC 2 cut(s) 155, 226
Mox20I TGGCCA 1 cut(s) 10
Mph1103I ATGCAT 2 cut(s) 238, 271
MscI TGGCCA 1 cut(s) 10
MseI TTAA 3 cut(s) 263, 327, 473
Msp20I TGGCCA 1 cut(s) 10
MspA1I CMGCKG 1 cut(s) 573
MspR9I CCNGG 1 cut(s) 552
MunI CAATTG 1 cut(s) 537
MvaI CCWGG 1 cut(s) 552
MwoI GCNNNNNNNGC 2 cut(s) 35, 392
NdeII GATC 2 cut(s) 302, 354
NlaIV GGNNCC 1 cut(s) 547
NmuCI GTSAC 1 cut(s) 502
NsiI ATGCAT 2 cut(s) 238, 271
NspV TTCGAA 1 cut(s) 523
PfeI GAWTC 1 cut(s) 436
PkrI GCNGC 2 cut(s) 37, 388
PleI GAGTC 2 cut(s) 499, 651
PpsI GAGTC 2 cut(s) 499, 651
PshBI ATTAAT 1 cut(s) 473
Psp6I CCWGG 1 cut(s) 550
PspGI CCWGG 1 cut(s) 550
PspN4I GGNNCC 1 cut(s) 547
PsrI GAACNNNNNNTAC 2 cut(s) 233, 265
PstNI CAGNNNCTG 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 573
RsaI GTAC 3 cut(s) 104, 531, 564
RsaNI GTAC 3 cut(s) 103, 530, 563
SaqAI TTAA 3 cut(s) 263, 327, 473
SatI GCNGC 2 cut(s) 36, 387
Sau3AI GATC 2 cut(s) 302, 354
SchI GAGTC 2 cut(s) 499, 652
ScrFI CCNGG 1 cut(s) 552
SduI GDGCHC 1 cut(s) 550
SetI ASST 9 cut(s) 118, 166, 378, 388, 449, 492, 556, 568, 575
SfaNI GCATC 5 cut(s) 22, 40, 72, 304, 484
SfcI CTRYAG 1 cut(s) 318
SfuI TTCGAA 1 cut(s) 523
Sse9I AATT 6 cut(s) 181, 189, 196, 470, 525, 537
SspMI CTAG 1 cut(s) 491
StyD4I CCNGG 1 cut(s) 550
StyI CCWWGG 1 cut(s) 306
TaaI ACNGT 4 cut(s) 25, 129, 562, 652
TaiI ACGT 2 cut(s) 378, 568
TaqI TCGA 2 cut(s) 98, 523
TasI AATT 6 cut(s) 181, 189, 196, 470, 525, 537
TatI WGTACW 2 cut(s) 102, 529
TfiI GAWTC 1 cut(s) 436
Tru1I TTAA 3 cut(s) 263, 327, 473
Tru9I TTAA 3 cut(s) 263, 327, 473
TscAI CASTG 1 cut(s) 303
TseFI GTSAC 1 cut(s) 502
TseI GCWGC 2 cut(s) 35, 386
Tsp45I GTSAC 1 cut(s) 502
TspDTI ATGAA 2 cut(s) 170, 240
TspRI CASTG 1 cut(s) 303
VspI ATTAAT 1 cut(s) 473
XmiI GTMKAC 1 cut(s) 177
XspI CTAG 1 cut(s) 491
Zsp2I ATGCAT 2 cut(s) 238, 271
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.