pycom17g11700

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0000427
Physical Location & Seq
Reverse (-)
9442010 .. 9442568
559 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g11700.2

Sequence Viewer

Length: 519 bp
ATGGCTACCACCAATTCAAGGCAGAGACTTTTCTCTCTCCTCTCGTTTCTTATTCTAATTCTTCAATCCAAAATCTCATCTGGGTCCGAAACCCCAATTGAAGAATCCACCATTGACGACCTCCAGATCGCTTTCAAGCTCAAGACTCTGACTTCATGGCAACTGGTTCAGTTCTACCTGGACAGAATCCAAACCCTCAACAGCCACCTTAGAGGGGTCCTGGAGGTGAACCCAGATGCGCTTTCCCAAGCCGATTCCGCCGACGAACAACGCCGGAAATGGGAATCGAACCCATCAGCCAGAACCAGAGCACTGCCGAAGCTGCACGGGATTCCCATTTTGCTCAAGGACAACATTGGCACCAAGGACAAACTTAACAACACCGTCGGCTCCTTTGCGCTGTTGGGTTCCAAAGTTCCGAGGGATGCCGGGGTTGTGGCGAAGCTGAGGAGCTCTGGGGCTGTGATTCTGGGGAAGGGTAGCTTGAGCCAGTGGTCGTATATCCGGTCGGTGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

173

Amino Acids

19.06

Weight (kDa)

9.92

Isoelectric Point (pI)

42.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 359
AciI CCGC 1 cut(s) 258
AfiI CCNNNNNNNGG 3 cut(s) 18, 214, 280
AgsI TTSAA 4 cut(s) 18, 65, 101, 136
AjnI CCWGG 2 cut(s) 177, 219
AluBI AGCT 5 cut(s) 139, 322, 445, 453, 483
AluI AGCT 5 cut(s) 139, 322, 445, 453, 483
Alw21I GWGCWC 2 cut(s) 313, 455
Alw26I GTCTC 1 cut(s) 19
ApeKI GCWGC 1 cut(s) 322
AspLEI GCGC 2 cut(s) 241, 400
AspS9I GGNCC 2 cut(s) 84, 217
AsuC2I CCSGG 1 cut(s) 430
AsuHPI GGTGA 1 cut(s) 238
AvaII GGWCC 2 cut(s) 84, 217
BanI GGYRCC 1 cut(s) 359
BanII GRGCYC 1 cut(s) 455
Bbv12I GWGCWC 2 cut(s) 313, 455
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 1 cut(s) 309
BccI CCATC 1 cut(s) 301
BciT130I CCWGG 2 cut(s) 179, 221
BcnI CCSGG 1 cut(s) 430
BcoDI GTCTC 1 cut(s) 19
BisI GCNGC 1 cut(s) 323
BlsI GCNGC 1 cut(s) 324
Bme1390I CCNGG 3 cut(s) 179, 221, 430
Bme18I GGWCC 2 cut(s) 84, 217
BmgT120I GGNCC 2 cut(s) 84, 217
BmiI GGNNCC 5 cut(s) 85, 218, 361, 391, 409
BmrFI CCNGG 3 cut(s) 179, 221, 430
BmsI GCATC 2 cut(s) 226, 415
BpmI CTGGAG 2 cut(s) 107, 242
Bpu10I CCTNAGC 1 cut(s) 446
BpuEI CTTGAG 3 cut(s) 125, 329, 505
BpuMI CCSGG 1 cut(s) 430
BsaJI CCNNGG 3 cut(s) 363, 419, 429
BsaWI WCCGGW 1 cut(s) 504
Bsc4I CCNNNNNNNGG 3 cut(s) 18, 214, 280
Bse1I ACTGG 2 cut(s) 168, 490
BseBI CCWGG 2 cut(s) 179, 221
BseDI CCNNGG 3 cut(s) 363, 419, 429
BseGI GGATG 1 cut(s) 430
BseLI CCNNNNNNNGG 3 cut(s) 18, 214, 280
BseMII CTCAG 1 cut(s) 437
BseNI ACTGG 2 cut(s) 168, 490
BseRI GAGGAG 2 cut(s) 29, 463
BseXI GCAGC 1 cut(s) 309
BsgI GTGCAG 1 cut(s) 308
Bsh1285I CGRYCG 1 cut(s) 509
BshNI GGYRCC 1 cut(s) 359
BsiEI CGRYCG 1 cut(s) 509
BsiHKAI GWGCWC 2 cut(s) 313, 455
BsiSI CCGG 3 cut(s) 274, 429, 505
BslI CCNNNNNNNGG 3 cut(s) 18, 214, 280
BsmAI GTCTC 1 cut(s) 19
Bsp1286I GDGCHC 2 cut(s) 313, 455
Bsp143I GATC 1 cut(s) 126
BspACI CCGC 1 cut(s) 258
BspCNI CTCAG 1 cut(s) 438
BspLI GGNNCC 5 cut(s) 85, 218, 361, 391, 409
BspT107I GGYRCC 1 cut(s) 359
BsrI ACTGG 2 cut(s) 168, 490
BssECI CCNNGG 3 cut(s) 363, 419, 429
BssMI GATC 1 cut(s) 126
BssT1I CCWWGG 1 cut(s) 363
Bst2UI CCWGG 2 cut(s) 179, 221
Bst4CI ACNGT 1 cut(s) 385
BstDEI CTNAG 2 cut(s) 209, 446
BstF5I GGATG 1 cut(s) 430
BstHHI GCGC 2 cut(s) 241, 400
BstKTI GATC 1 cut(s) 129
BstMAI GTCTC 1 cut(s) 19
BstMBI GATC 1 cut(s) 126
BstMCI CGRYCG 1 cut(s) 509
BstMWI GCNNNNNNNGC 2 cut(s) 257, 322
BstNI CCWGG 2 cut(s) 179, 221
BstSCI CCNGG 3 cut(s) 177, 219, 428
BstV1I GCAGC 1 cut(s) 309
BtsCI GGATG 1 cut(s) 430
BtsI GCAGTG 1 cut(s) 311
BtsIMutI CAGTG 2 cut(s) 311, 497
CfoI GCGC 2 cut(s) 241, 400
Cfr13I GGNCC 2 cut(s) 84, 217
CviAII CATG 1 cut(s) 156
DdeI CTNAG 2 cut(s) 209, 446
DpnI GATC 1 cut(s) 128
DpnII GATC 1 cut(s) 126
EciI GGCGGA 1 cut(s) 247
Ecl136II GAGCTC 1 cut(s) 453
Eco130I CCWWGG 1 cut(s) 363
Eco24I GRGCYC 1 cut(s) 455
Eco47I GGWCC 2 cut(s) 84, 217
Eco53kI GAGCTC 1 cut(s) 453
EcoICRI GAGCTC 1 cut(s) 453
EcoO109I RGGNCCY 1 cut(s) 217
EcoRII CCWGG 2 cut(s) 177, 219
EcoT14I CCWWGG 1 cut(s) 363
EcoT38I GRGCYC 1 cut(s) 455
ErhI CCWWGG 1 cut(s) 363
FaeI CATG 1 cut(s) 159
FaiI YATR 2 cut(s) 157, 501
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FatI CATG 1 cut(s) 155
Fnu4HI GCNGC 1 cut(s) 323
FokI GGATG 1 cut(s) 437
FriOI GRGCYC 1 cut(s) 455
Fsp4HI GCNGC 1 cut(s) 323
GlaI GCGC 2 cut(s) 240, 399
GluI GCNGC 1 cut(s) 323
GsuI CTGGAG 2 cut(s) 107, 242
HapII CCGG 3 cut(s) 274, 429, 505
HhaI GCGC 2 cut(s) 241, 400
Hin1II CATG 1 cut(s) 159
Hin6I GCGC 2 cut(s) 239, 398
HinP1I GCGC 2 cut(s) 239, 398
HinfI GANTC 7 cut(s) 104, 145, 186, 254, 284, 331, 466
HpaII CCGG 3 cut(s) 274, 429, 505
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 3 cut(s) 88, 150, 420
Hpy188III TCNNGA 2 cut(s) 124, 142
Hpy8I GTNNAC 1 cut(s) 229
Hpy99I CGWCG 2 cut(s) 266, 389
HpyAV CCTTC 1 cut(s) 469
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4V TGCA 1 cut(s) 325
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 322
HpyF3I CTNAG 2 cut(s) 209, 446
Hsp92II CATG 1 cut(s) 159
HspAI GCGC 2 cut(s) 239, 398
Kzo9I GATC 1 cut(s) 126
LmnI GCTCC 2 cut(s) 395, 450
Lsp1109I GCAGC 1 cut(s) 309
LweI GCATC 2 cut(s) 226, 415
MalI GATC 1 cut(s) 128
MboI GATC 1 cut(s) 126
MboII GAAGA 2 cut(s) 53, 113
MfeI CAATTG 1 cut(s) 96
MhlI GDGCHC 2 cut(s) 313, 455
MluCI AATT 3 cut(s) 13, 57, 96
MlyI GAGTC 1 cut(s) 139
MnlI CCTC 7 cut(s) 50, 131, 206, 206, 217, 414, 441
MseI TTAA 1 cut(s) 375
MspI CCGG 3 cut(s) 274, 429, 505
MspR9I CCNGG 3 cut(s) 179, 221, 430
MunI CAATTG 1 cut(s) 96
MvaI CCWGG 2 cut(s) 179, 221
MwoI GCNNNNNNNGC 2 cut(s) 257, 322
NciI CCSGG 1 cut(s) 430
NdeII GATC 1 cut(s) 126
NlaIII CATG 1 cut(s) 159
NlaIV GGNNCC 5 cut(s) 85, 218, 361, 391, 409
PfeI GAWTC 6 cut(s) 104, 186, 254, 284, 331, 466
PfoI TCCNGGA 1 cut(s) 219
PkrI GCNGC 1 cut(s) 324
PleI GAGTC 1 cut(s) 139
PpsI GAGTC 1 cut(s) 139
PpuMI RGGWCCY 1 cut(s) 217
Psp124BI GAGCTC 1 cut(s) 455
Psp5II RGGWCCY 1 cut(s) 217
Psp6I CCWGG 2 cut(s) 177, 219
PspGI CCWGG 2 cut(s) 177, 219
PspN4I GGNNCC 5 cut(s) 85, 218, 361, 391, 409
PspPI GGNCC 2 cut(s) 84, 217
PspPPI RGGWCCY 1 cut(s) 217
SacI GAGCTC 1 cut(s) 455
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 1 cut(s) 323
Sau3AI GATC 1 cut(s) 126
Sau96I GGNCC 2 cut(s) 84, 217
SchI GAGTC 1 cut(s) 139
ScrFI CCNGG 3 cut(s) 179, 221, 430
SduI GDGCHC 2 cut(s) 313, 455
SetI ASST 9 cut(s) 123, 141, 180, 210, 228, 324, 447, 455, 485
SfaNI GCATC 2 cut(s) 226, 415
SinI GGWCC 2 cut(s) 84, 217
SmlI CTYRAG 3 cut(s) 140, 344, 484
SmoI CTYRAG 3 cut(s) 140, 344, 484
Sse9I AATT 3 cut(s) 13, 57, 96
SsiI CCGC 1 cut(s) 258
SstI GAGCTC 1 cut(s) 455
StyD4I CCNGG 3 cut(s) 177, 219, 428
StyI CCWWGG 1 cut(s) 363
TaaI ACNGT 1 cut(s) 385
TaqI TCGA 1 cut(s) 287
TasI AATT 3 cut(s) 13, 57, 96
TfiI GAWTC 6 cut(s) 104, 186, 254, 284, 331, 466
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 318, 497
TseI GCWGC 1 cut(s) 322
TspDTI ATGAA 1 cut(s) 144
TspRI CASTG 2 cut(s) 318, 497
VpaK11BI GGWCC 2 cut(s) 84, 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.