Rh6BG021000

amidase C869.01

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0000427
Physical Location & Seq
Reverse (-)
3092648 .. 3093271
624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG021000.1

Sequence Viewer

Length: 624 bp
ATGGCCAAAACTCCGTTGATCAGCTCAAATTCTTGGGTTGTTGGTCACTGGCAGTCTGCCACTCCATTACTGCCATTATCATCCAAGGCGAACTATCTTCTCCTCTCGTTTTTGCTTCTAATTTGCATTCTATCATATGGCTCACAAAAACTCACGGCCAAAAGGCCCAAAATCACAATCGAAGAAGCTACTGTTGATGATCTCCAGCTTGCTTTCAAGCTCAACACTCTGACCTCGAGGCAACTTGTTCTGTTCTACATGGATGAGATTCAAAAACTGAACAAAAACCTTAGGGCTGTGATAGAGGTGAACCCTGATGCTTTATCCCAAGCTGATATAGCTGACAAACAACGCAGAGAATGCAACCACACACTGCCTAAGCTTCATGGCATTCCTATTCTGCTCAAGGATACCTTAGCAACCAAGGACAAACTCAACACCACAGCTGGATCGTTTGCCTTGTTGGGGTCCATTGTTCCTCGAGATGCAGGTGTTGTTTCTAAGCTAAGGAGGTCTGGAGCTATCATTTTAGGGAAGGCTAGCTTGAGTGAATGGTGCCATTTCAGGTCATTCCTAGCACCTTCTGGATGGAGCGCCAGAGGTTCCCAAGGGATGGTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

207

Amino Acids

22.73

Weight (kDa)

9.74

Isoelectric Point (pI)

45.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Amidase PF01425 82 - 188 9.8e-24 Amidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 479
AbsI CCTCGAGG 1 cut(s) 235
Acc36I ACCTGC 1 cut(s) 479
AccB1I GGYRCC 1 cut(s) 555
AccB7I CCANNNNNTGG 1 cut(s) 613
AclWI GGATC 1 cut(s) 457
AcoI YGGCCR 2 cut(s) 3, 156
AcsI RAATTY 1 cut(s) 28
AfiI CCNNNNNNNGG 2 cut(s) 465, 613
AgsI TTSAA 2 cut(s) 217, 272
AlwI GGATC 1 cut(s) 457
Ama87I CYCGRG 2 cut(s) 235, 480
AoxI GGCC 3 cut(s) 3, 156, 164
ApoI RAATTY 1 cut(s) 28
AspLEI GCGC 1 cut(s) 596
AspS9I GGNCC 2 cut(s) 165, 468
AsuHPI GGTGA 1 cut(s) 319
AsuNHI GCTAGC 1 cut(s) 539
AvaI CYCGRG 2 cut(s) 235, 480
AvaII GGWCC 1 cut(s) 468
AxyI CCTNAGG 1 cut(s) 290
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 555
BccI CCATC 2 cut(s) 582, 607
BceAI ACGGC 1 cut(s) 171
BciVI GTATCC 1 cut(s) 403
BclI TGATCA 1 cut(s) 18
BfaI CTAG 2 cut(s) 540, 575
BfoI RGCGCY 1 cut(s) 597
BfuAI ACCTGC 1 cut(s) 479
BfuI GTATCC 1 cut(s) 403
Bme18I GGWCC 1 cut(s) 468
BmeT110I CYCGRG 2 cut(s) 235, 480
BmgT120I GGNCC 2 cut(s) 165, 468
BmiI GGNNCC 3 cut(s) 469, 557, 604
BmsI GCATC 2 cut(s) 307, 475
BmtI GCTAGC 1 cut(s) 543
BpmI CTGGAG 2 cut(s) 188, 537
Bpu10I CCTNAGC 3 cut(s) 378, 415, 506
BpuEI CTTGAG 2 cut(s) 389, 565
BsaJI CCNNGG 3 cut(s) 84, 423, 607
Bsc4I CCNNNNNNNGG 2 cut(s) 465, 613
Bse1I ACTGG 1 cut(s) 53
Bse21I CCTNAGG 1 cut(s) 290
BseDI CCNNGG 3 cut(s) 84, 423, 607
BseGI GGATG 4 cut(s) 80, 268, 593, 618
BseLI CCNNNNNNNGG 2 cut(s) 465, 613
BseNI ACTGG 1 cut(s) 53
BseRI GAGGAG 1 cut(s) 92
BshFI GGCC 3 cut(s) 5, 158, 166
BshNI GGYRCC 1 cut(s) 555
BsiHKCI CYCGRG 2 cut(s) 235, 480
BslI CCNNNNNNNGG 2 cut(s) 465, 613
BsmI GAATGC 3 cut(s) 126, 365, 390
BsnI GGCC 3 cut(s) 5, 158, 166
BsoBI CYCGRG 2 cut(s) 235, 480
Bsp143I GATC 3 cut(s) 18, 199, 449
BspANI GGCC 3 cut(s) 5, 158, 166
BspLI GGNNCC 3 cut(s) 469, 557, 604
BspMI ACCTGC 1 cut(s) 479
BspOI GCTAGC 1 cut(s) 543
BspPI GGATC 1 cut(s) 457
BspT107I GGYRCC 1 cut(s) 555
BsrI ACTGG 1 cut(s) 53
BssECI CCNNGG 3 cut(s) 84, 423, 607
BssMI GATC 3 cut(s) 18, 199, 449
BssT1I CCWWGG 3 cut(s) 84, 423, 607
Bst4CI ACNGT 1 cut(s) 193
BstAPI GCANNNNNTGC 1 cut(s) 360
BstC8I GCNNGC 2 cut(s) 210, 541
BstDEI CTNAG 5 cut(s) 290, 378, 415, 501, 506
BstF5I GGATG 4 cut(s) 80, 268, 593, 618
BstH2I RGCGCY 1 cut(s) 597
BstHHI GCGC 1 cut(s) 596
BstKTI GATC 3 cut(s) 21, 202, 452
BstMBI GATC 3 cut(s) 18, 199, 449
BstMWI GCNNNNNNNGC 2 cut(s) 338, 360
Bsu36I CCTNAGG 1 cut(s) 290
BsuI GTATCC 1 cut(s) 403
BsuRI GGCC 3 cut(s) 5, 158, 166
BtsCI GGATG 4 cut(s) 80, 268, 593, 618
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 2 cut(s) 46, 371
BveI ACCTGC 1 cut(s) 479
Cac8I GCNNGC 2 cut(s) 210, 541
CfoI GCGC 1 cut(s) 596
Cfr13I GGNCC 2 cut(s) 165, 468
CviAII CATG 2 cut(s) 259, 386
DdeI CTNAG 5 cut(s) 290, 378, 415, 501, 506
DpnI GATC 3 cut(s) 20, 201, 451
DpnII GATC 3 cut(s) 18, 199, 449
EaeI YGGCCR 2 cut(s) 3, 156
Eco130I CCWWGG 3 cut(s) 84, 423, 607
Eco47I GGWCC 1 cut(s) 468
Eco81I CCTNAGG 1 cut(s) 290
Eco88I CYCGRG 2 cut(s) 235, 480
EcoT14I CCWWGG 3 cut(s) 84, 423, 607
ErhI CCWWGG 3 cut(s) 84, 423, 607
FaeI CATG 2 cut(s) 262, 389
FaiI YATR 5 cut(s) 136, 138, 260, 338, 387
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 527, 559
FatI CATG 2 cut(s) 258, 385
FauNDI CATATG 1 cut(s) 136
FbaI TGATCA 1 cut(s) 18
FokI GGATG 3 cut(s) 67, 275, 600
FspBI CTAG 2 cut(s) 540, 575
GlaI GCGC 1 cut(s) 595
GsuI CTGGAG 2 cut(s) 188, 537
HaeII RGCGCY 1 cut(s) 597
HaeIII GGCC 3 cut(s) 5, 158, 166
HhaI GCGC 1 cut(s) 596
Hin1II CATG 2 cut(s) 262, 389
Hin6I GCGC 1 cut(s) 594
HinP1I GCGC 1 cut(s) 594
HindIII AAGCTT 1 cut(s) 380
HinfI GANTC 1 cut(s) 268
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 1 cut(s) 310
Hpy188I TCNGA 1 cut(s) 231
Hpy188III TCNNGA 3 cut(s) 482, 516, 585
Hpy8I GTNNAC 1 cut(s) 310
HpyAV CCTTC 2 cut(s) 529, 591
HpyCH4III ACNGT 1 cut(s) 193
HpyCH4V TGCA 3 cut(s) 126, 363, 488
HpyF10VI GCNNNNNNNGC 2 cut(s) 338, 360
HpyF3I CTNAG 5 cut(s) 290, 378, 415, 501, 506
Hsp92II CATG 2 cut(s) 262, 389
HspAI GCGC 1 cut(s) 594
Ksp22I TGATCA 1 cut(s) 18
Kzo9I GATC 3 cut(s) 18, 199, 449
LmnI GCTCC 2 cut(s) 518, 591
LpnPI CCDG 9 cut(s) 34, 218, 327, 432, 474, 501, 550, 570, 610
LweI GCATC 2 cut(s) 307, 475
MaeI CTAG 2 cut(s) 540, 575
MaeIII GTNAC 1 cut(s) 44
MalI GATC 3 cut(s) 20, 201, 451
MboI GATC 3 cut(s) 18, 199, 449
MboII GAAGA 2 cut(s) 89, 194
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 28, 120
MluNI TGGCCA 1 cut(s) 5
MnlI CCTC 7 cut(s) 113, 231, 244, 298, 489, 504, 593
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 622
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 446
Mva1269I GAATGC 3 cut(s) 126, 365, 390
MwoI GCNNNNNNNGC 2 cut(s) 338, 360
NdeI CATATG 1 cut(s) 136
NdeII GATC 3 cut(s) 18, 199, 449
NheI GCTAGC 1 cut(s) 539
NlaIII CATG 2 cut(s) 262, 389
NlaIV GGNNCC 3 cut(s) 469, 557, 604
NmuCI GTSAC 1 cut(s) 44
PaeR7I CTCGAG 2 cut(s) 235, 480
PaqCI CACCTGC 1 cut(s) 479
PctI GAATGC 3 cut(s) 126, 365, 390
PfeI GAWTC 1 cut(s) 268
PflMI CCANNNNNTGG 1 cut(s) 613
PspN4I GGNNCC 3 cut(s) 469, 557, 604
PspPI GGNCC 2 cut(s) 165, 468
PspXI VCTCGAGB 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 446
SaqAI TTAA 1 cut(s) 622
Sau3AI GATC 3 cut(s) 18, 199, 449
Sau96I GGNCC 2 cut(s) 165, 468
SfaNI GCATC 2 cut(s) 307, 475
Sfr274I CTCGAG 2 cut(s) 235, 480
SinI GGWCC 1 cut(s) 468
SlaI CTCGAG 2 cut(s) 235, 480
SmlI CTYRAG 4 cut(s) 235, 404, 480, 544
SmoI CTYRAG 4 cut(s) 235, 404, 480, 544
Sse9I AATT 2 cut(s) 28, 120
SspMI CTAG 2 cut(s) 540, 575
StyI CCWWGG 3 cut(s) 84, 423, 607
TaaI ACNGT 1 cut(s) 193
TaqI TCGA 3 cut(s) 180, 236, 481
TasI AATT 2 cut(s) 28, 120
TfiI GAWTC 1 cut(s) 268
Tru1I TTAA 1 cut(s) 622
Tru9I TTAA 1 cut(s) 622
TscAI CASTG 2 cut(s) 53, 378
TseFI GTSAC 1 cut(s) 44
Tsp45I GTSAC 1 cut(s) 44
TspDTI ATGAA 1 cut(s) 374
TspRI CASTG 2 cut(s) 53, 378
Van91I CCANNNNNTGG 1 cut(s) 613
VpaK11BI GGWCC 1 cut(s) 468
XapI RAATTY 1 cut(s) 28
XhoI CTCGAG 2 cut(s) 235, 480
XspI CTAG 2 cut(s) 540, 575
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.