Rorug05G0510400

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000427
Physical Location & Seq
Reverse (-)
69440987 .. 69441672
686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0510400.1

Sequence Viewer

Length: 444 bp
ATGATCTTCGGCCTCGGCTCCGGCTCCACCGTCAAACACGCCCTGGATCGCTTGGGCGAGCTTTTACAACTGGAAAAAGTTCACAACATCATCAGAATTTCTGAGAACACCCACCAACAAGTCATCTCTCTTAGAATTCCATTATCCAACCTCGATGACTACCCGATCCTCGATCTCGCTATCGACGGTGCCGACGAGGTGGACCCCCACCTCAACCTAGTCAAAGGCCGCTGCGGCCTTGATAATTTGGATGATAGCCTGAAGGGGAATGTTTTGGAATTGAAGGAAGAGTTGGACTCTGAATTTGTTAGTGTCCTCAAGTCCTTGGGGTTGGATGTGGAGATAAGATCAAAAGAATTCATTGAGCAAACTTTGCCCTCAGAAGTCAAGGCTAGATTTTTTTTTGCTGGGTTCAAGAACATGAATGAATCAACATTGTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

147

Amino Acids

16.42

Weight (kDa)

4.77

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rib_5-P_isom_A PF06026 31 - 79 3.2e-12 Ribose 5-phosphate isomerase A (phosphoriboisomerase A)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 188
AciI CCGC 2 cut(s) 229, 234
AclWI GGATC 2 cut(s) 54, 160
AcsI RAATTY 4 cut(s) 96, 135, 302, 356
AcuI CTGAAG 1 cut(s) 281
AgsI TTSAA 2 cut(s) 283, 415
AjnI CCWGG 1 cut(s) 42
AjuI GAANNNNNNNTTGG 2 cut(s) 275, 307
AluBI AGCT 1 cut(s) 61
AluI AGCT 1 cut(s) 61
AlwI GGATC 2 cut(s) 54, 160
AoxI GGCC 3 cut(s) 10, 226, 235
ApeKI GCWGC 1 cut(s) 231
ApoI RAATTY 4 cut(s) 96, 135, 302, 356
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
Asp700I GAANNNNTTC 1 cut(s) 78
AspS9I GGNCC 1 cut(s) 202
AvaII GGWCC 1 cut(s) 202
BanI GGYRCC 1 cut(s) 188
BbvI GCAGC 1 cut(s) 218
BciT130I CCWGG 1 cut(s) 44
BfaI CTAG 2 cut(s) 218, 393
BglI GCCNNNNNGGC 1 cut(s) 234
BisI GCNGC 3 cut(s) 229, 232, 235
BlsI GCNGC 3 cut(s) 230, 233, 236
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 1 cut(s) 202
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 4 cut(s) 19, 25, 190, 204
BmrFI CCNGG 1 cut(s) 44
BplI GAGNNNNNCTC 2 cut(s) 281, 313
BpuEI CTTGAG 1 cut(s) 302
BsaJI CCNNGG 3 cut(s) 13, 42, 324
Bse1I ACTGG 1 cut(s) 75
BseBI CCWGG 1 cut(s) 44
BseDI CCNNGG 3 cut(s) 13, 42, 324
BseGI GGATG 2 cut(s) 256, 340
BseMII CTCAG 2 cut(s) 93, 393
BseNI ACTGG 1 cut(s) 75
BseXI GCAGC 1 cut(s) 218
BseYI CCCAGC 1 cut(s) 407
BshFI GGCC 3 cut(s) 12, 228, 237
BshNI GGYRCC 1 cut(s) 188
BsiSI CCGG 1 cut(s) 21
BsnI GGCC 3 cut(s) 12, 228, 237
Bsp143I GATC 5 cut(s) 3, 46, 165, 172, 347
BspACI CCGC 2 cut(s) 229, 234
BspANI GGCC 3 cut(s) 12, 228, 237
BspCNI CTCAG 2 cut(s) 94, 392
BspLI GGNNCC 4 cut(s) 19, 25, 190, 204
BspPI GGATC 2 cut(s) 54, 160
BspT107I GGYRCC 1 cut(s) 188
BsrI ACTGG 1 cut(s) 75
BssECI CCNNGG 3 cut(s) 13, 42, 324
BssMI GATC 5 cut(s) 3, 46, 165, 172, 347
BssT1I CCWWGG 1 cut(s) 324
Bst2UI CCWGG 1 cut(s) 44
Bst4CI ACNGT 2 cut(s) 31, 188
Bst6I CTCTTC 1 cut(s) 282
BstAPI GCANNNNNTGC 1 cut(s) 373
BstC8I GCNNGC 1 cut(s) 59
BstDEI CTNAG 3 cut(s) 102, 131, 379
BstF5I GGATG 2 cut(s) 256, 340
BstKTI GATC 5 cut(s) 6, 49, 168, 175, 350
BstMBI GATC 5 cut(s) 3, 46, 165, 172, 347
BstMWI GCNNNNNNNGC 2 cut(s) 234, 373
BstNI CCWGG 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 42
BstV1I GCAGC 1 cut(s) 218
BsuRI GGCC 3 cut(s) 12, 228, 237
BtsCI GGATG 2 cut(s) 256, 340
Cac8I GCNNGC 1 cut(s) 59
Cfr13I GGNCC 1 cut(s) 202
CviAII CATG 1 cut(s) 421
CviJI RGCY 8 cut(s) 12, 18, 24, 61, 228, 237, 258, 392
CviKI_1 RGCY 8 cut(s) 12, 18, 24, 61, 228, 237, 258, 392
DdeI CTNAG 3 cut(s) 102, 131, 379
DpnI GATC 5 cut(s) 5, 48, 167, 174, 349
DpnII GATC 5 cut(s) 3, 46, 165, 172, 347
Eam1104I CTCTTC 1 cut(s) 282
EarI CTCTTC 1 cut(s) 282
Eco130I CCWWGG 1 cut(s) 324
Eco47I GGWCC 1 cut(s) 202
Eco57I CTGAAG 1 cut(s) 281
EcoRI GAATTC 2 cut(s) 135, 356
EcoRII CCWGG 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 324
FaeI CATG 1 cut(s) 424
FaiI YATR 1 cut(s) 422
FatI CATG 1 cut(s) 420
Fnu4HI GCNGC 3 cut(s) 229, 232, 235
FokI GGATG 2 cut(s) 263, 347
Fsp4HI GCNGC 3 cut(s) 229, 232, 235
FspBI CTAG 2 cut(s) 218, 393
GluI GCNGC 3 cut(s) 229, 232, 235
GsaI CCCAGC 1 cut(s) 411
HaeIII GGCC 3 cut(s) 12, 228, 237
HapII CCGG 1 cut(s) 21
Hin1II CATG 1 cut(s) 424
HinfI GANTC 2 cut(s) 296, 428
HpaII CCGG 1 cut(s) 21
Hpy166II GTNNAC 2 cut(s) 82, 202
Hpy188I TCNGA 4 cut(s) 95, 103, 301, 382
Hpy188III TCNNGA 1 cut(s) 415
Hpy8I GTNNAC 2 cut(s) 82, 202
Hpy99I CGWCG 2 cut(s) 188, 197
HpyAV CCTTC 2 cut(s) 256, 277
HpyCH4III ACNGT 2 cut(s) 31, 188
HpyF10VI GCNNNNNNNGC 2 cut(s) 234, 373
HpyF3I CTNAG 3 cut(s) 102, 131, 379
Hsp92II CATG 1 cut(s) 424
Kzo9I GATC 5 cut(s) 3, 46, 165, 172, 347
LmnI GCTCC 2 cut(s) 23, 29
LpnPI CCDG 6 cut(s) 29, 34, 56, 56, 272, 393
Lsp1109I GCAGC 1 cut(s) 218
MaeI CTAG 2 cut(s) 218, 393
MalI GATC 5 cut(s) 5, 48, 167, 174, 349
MboI GATC 5 cut(s) 3, 46, 165, 172, 347
MboII GAAGA 1 cut(s) 299
MluCI AATT 6 cut(s) 96, 135, 244, 278, 302, 356
MlyI GAGTC 1 cut(s) 290
MmeI TCCRAC 3 cut(s) 171, 273, 312
MnlI CCTC 7 cut(s) 23, 161, 179, 190, 221, 326, 388
MroXI GAANNNNTTC 1 cut(s) 78
MslI CAYNNNNRTG 1 cut(s) 439
MspA1I CMGCKG 1 cut(s) 231
MspI CCGG 1 cut(s) 21
MspR9I CCNGG 1 cut(s) 44
MvaI CCWGG 1 cut(s) 44
MwoI GCNNNNNNNGC 2 cut(s) 234, 373
NdeII GATC 5 cut(s) 3, 46, 165, 172, 347
NlaIII CATG 1 cut(s) 424
NlaIV GGNNCC 4 cut(s) 19, 25, 190, 204
PcsI WCGNNNNNNNCGW 2 cut(s) 189, 192
PdmI GAANNNNTTC 1 cut(s) 78
PfeI GAWTC 1 cut(s) 428
PkrI GCNGC 3 cut(s) 230, 233, 236
PleI GAGTC 1 cut(s) 290
PpsI GAGTC 1 cut(s) 290
Psp6I CCWGG 1 cut(s) 42
PspFI CCCAGC 1 cut(s) 407
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 4 cut(s) 19, 25, 190, 204
PspPI GGNCC 1 cut(s) 202
RseI CAYNNNNRTG 1 cut(s) 439
SatI GCNGC 3 cut(s) 229, 232, 235
Sau3AI GATC 5 cut(s) 3, 46, 165, 172, 347
Sau96I GGNCC 1 cut(s) 202
SchI GAGTC 1 cut(s) 290
ScrFI CCNGG 1 cut(s) 44
SetI ASST 5 cut(s) 63, 153, 201, 213, 219
SfiI GGCCNNNNNGGCC 1 cut(s) 234
SinI GGWCC 1 cut(s) 202
SmiMI CAYNNNNRTG 1 cut(s) 439
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 6 cut(s) 96, 135, 244, 278, 302, 356
SsiI CCGC 2 cut(s) 229, 234
SspMI CTAG 2 cut(s) 218, 393
StyD4I CCNGG 1 cut(s) 42
StyI CCWWGG 1 cut(s) 324
TaaI ACNGT 2 cut(s) 31, 188
TaqI TCGA 3 cut(s) 153, 171, 183
TasI AATT 6 cut(s) 96, 135, 244, 278, 302, 356
TauI GCSGC 2 cut(s) 231, 237
TfiI GAWTC 1 cut(s) 428
TseI GCWGC 1 cut(s) 231
TspDTI ATGAA 3 cut(s) 349, 437, 441
VpaK11BI GGWCC 1 cut(s) 202
XapI RAATTY 4 cut(s) 96, 135, 302, 356
XmnI GAANNNNTTC 1 cut(s) 78
XspI CTAG 2 cut(s) 218, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.