Prupe.8G161900_v2.0.a1

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0000427
Physical Location & Seq
Forward (+)
16984665 .. 16987374
2710 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G161900.1

Sequence Viewer

Length: 1560 bp
ATGGCTGCAAAATCACCATCATGCTTTCCTCTCTTCCCATCTTTGCTTCTCATTCTTCTGTCCTTGGCTTCATTTGGGTCCCAAACTTTTTCTGTCCATGGGCTCTATATTAGAGAAGCTACAATCTCTGATCTCCAACTAGCTTTCAAACAGAACCAACTGACTTCAAGAAAACTTGTTGAGTTCTACCTTCAAGAAATCCGCAGGCTCAACCCGGTCCTTAATGCAGTCATAGAGGTGAACCCAGATGCCCTTTACCAAGCTGATAAGGCAGACTATCAGCGAAAGGCAAAGGGCCCCGGTTACTATTCTGGGTTACATGGCATTCCTATCTTGCTCAAGGACAACATTGGAACCAAAGATAAGCTTAACACCACTGCTGGCTCGTTGGCTCTGCTTGGGTCAGTTGTGCCTCATGATGCCGGTGTGGTGAGCAAGTTGAGGAGCGCGGGAGCCATCATATTGGGCAAGGCTAGCTTAAGTGAGTGGGCTCAGTTTAGGTCTCTCGCAGCACCCAGTGGTTGGAGTGCCAGAGGTGGCCAAGGAAAGAACCCTTATGTGCTATCAGCAACTCCCTGTGGATCAAGCAGTGGCCCAGCAATTTCAGCTGCAGCAAATTTGGTAGCAGTATCTCTGGGGACTGAGACTGATGGCTCCATATTATGTCCAGCAAGTTTCAACTCAGTTGTGGGCATCAAACCAACAGTTGGCCTCACCAGCCGTGCAGGGGTGATTCCGGTAACTCCCAGACAGGACACCGTTGGGCCCATCACCAGGACAGTTTCAGATGCCGTTCATGTTCTTGATGCCATTGTAGGCTATGATTACAATGATCAAGCAACCAGAGAAGCCTCCAAGTACATTCCAAGCGGTGGCTACAAACAATTTCTTCAGGCGTACGGGCTCAAAGGAAAGAGACTTGGAATAGTGAGGGATCCCTTCTTCACTTCCAGCAGCGGATCTCTCCAGCTTCAAGCGTTTGAGAAACATTTCCAGACTCTAAGGCAAGGAGGTGCTGTTTTGGTAGACCATTTACAAATAGCCAACATTGATGTTATCTTGAACTTCAATTTGAGTGGGGAAGCAACAGCATCACTGGCTGAGTTCAAGCTAGCATTGAATGCATATCTAAAAGACCTGGTGGTATCTCCGGTTCGATCATTGGCAGATGTAATCGCCTTCAACCTCAAGTTCTCGGGTGTGGAATTGATCAAGGAATTTGGACAAGACATTTTTCTTGCTGCCCAAGCAACAAATGGGATAGGCAACAAAGAAAAGGCAGCATTGTTGAATCTAGCAAAGCTGACAAAATATGGGTTTGAAAAATTGTTGACAGACAACAGACTAGATGCATTGGTGACTCCTGGTTCAGATGTTTCCCCTGCGCTTGCAATTGGGGGTTTCCCAGGAATTAGTGTCCCTGCTGGATATGACAAGAAGGGTGTGCCCTTTGGCATAACCTTTGGGGGACTGAAGGGTTCTGAGCCAAAGCTGATCCAGATTGCCTACGGCTTTGAGCAAGCTACTAAAGTTAGGAAGGCTCCTACATTTTTGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

520

Amino Acids

54.99

Weight (kDa)

9.35

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 522, 707, 872
AccI GTMKAC 1 cut(s) 1026
AccII CGCG 1 cut(s) 449
AciI CCGC 4 cut(s) 202, 449, 870, 957
AclWI GGATC 5 cut(s) 589, 929, 942, 967, 1489
AcoI YGGCCR 1 cut(s) 538
AcsI RAATTY 2 cut(s) 616, 1217
AcuI CTGAAG 2 cut(s) 875, 1493
AdeI CACNNNGTG 1 cut(s) 518
AfaI GTAC 2 cut(s) 860, 899
AfiI CCNNNNNNNGG 5 cut(s) 522, 707, 727, 774, 872
AflII CTTAAG 1 cut(s) 478
AjnI CCWGG 4 cut(s) 773, 1137, 1363, 1405
Alw26I GTCTC 3 cut(s) 507, 638, 910
AlwI GGATC 5 cut(s) 589, 929, 942, 967, 1489
Ama87I CYCGRG 1 cut(s) 1195
AoxI GGCC 5 cut(s) 295, 538, 592, 709, 764
ApaI GGGCCC 2 cut(s) 299, 768
ApeKI GCWGC 7 cut(s) 5, 509, 608, 611, 954, 1241, 1280
ApoI RAATTY 2 cut(s) 616, 1217
Asp700I GAANNNNTTC 1 cut(s) 989
AspLEI GCGC 2 cut(s) 449, 1387
AspS9I GGNCC 7 cut(s) 78, 217, 295, 296, 593, 764, 765
AsuC2I CCSGG 2 cut(s) 215, 300
AsuHPI GGTGA 7 cut(s) 6, 250, 442, 706, 742, 763, 1369
AsuNHI GCTAGC 2 cut(s) 473, 1111
AvaI CYCGRG 1 cut(s) 1195
AvaII GGWCC 2 cut(s) 78, 217
BaeGI GKGCMC 3 cut(s) 299, 768, 1449
BalI TGGCCA 1 cut(s) 540
BamHI GGATCC 1 cut(s) 934
BanII GRGCYC 5 cut(s) 105, 299, 493, 768, 906
BbvI GCAGC 6 cut(s) 521, 595, 623, 966, 1228, 1292
BccI CCATC 5 cut(s) 25, 46, 464, 644, 776
BceAI ACGGC 3 cut(s) 705, 776, 1525
BciT130I CCWGG 4 cut(s) 775, 1139, 1365, 1407
BclI TGATCA 2 cut(s) 832, 1209
BcnI CCSGG 2 cut(s) 215, 300
BcoDI GTCTC 3 cut(s) 507, 638, 910
BfaI CTAG 5 cut(s) 140, 474, 1112, 1295, 1346
BfmI CTRYAG 1 cut(s) 609
BfrI CTTAAG 1 cut(s) 478
BisI GCNGC 7 cut(s) 6, 510, 609, 612, 955, 1242, 1281
BlsI GCNGC 7 cut(s) 7, 511, 610, 613, 956, 1243, 1282
Bme1390I CCNGG 6 cut(s) 215, 300, 775, 1139, 1365, 1407
Bme18I GGWCC 2 cut(s) 78, 217
BmeT110I CYCGRG 1 cut(s) 1195
BmgT120I GGNCC 7 cut(s) 78, 217, 295, 296, 593, 764, 765
BmrFI CCNGG 6 cut(s) 215, 300, 775, 1139, 1365, 1407
BmrI ACTGGG 1 cut(s) 510
BmsI GCATC 7 cut(s) 238, 409, 702, 778, 796, 1100, 1339
BmtI GCTAGC 2 cut(s) 477, 1115
BmuI ACTGGG 1 cut(s) 510
BpmI CTGGAG 1 cut(s) 950
BpuEI CTTGAG 2 cut(s) 323, 1172
BpuMI CCSGG 2 cut(s) 215, 300
BsaI GGTCTC 1 cut(s) 507
BsaJI CCNNGG 5 cut(s) 63, 97, 298, 541, 1405
BsaWI WCCGGW 2 cut(s) 736, 1150
BsaXI ACNNNNNCTCC 2 cut(s) 1002, 1032
Bsc4I CCNNNNNNNGG 5 cut(s) 522, 707, 727, 774, 872
Bse118I RCCGGY 1 cut(s) 422
Bse1I ACTGG 2 cut(s) 516, 1101
BseBI CCWGG 4 cut(s) 775, 1139, 1365, 1407
BseDI CCNNGG 5 cut(s) 63, 97, 298, 541, 1405
BseLI CCNNNNNNNGG 5 cut(s) 522, 707, 727, 774, 872
BseMII CTCAG 5 cut(s) 506, 633, 696, 1092, 1473
BseNI ACTGG 2 cut(s) 516, 1101
BseRI GAGGAG 1 cut(s) 457
BseSI GKGCMC 3 cut(s) 299, 768, 1449
BseXI GCAGC 6 cut(s) 521, 595, 623, 966, 1228, 1292
BseYI CCCAGC 1 cut(s) 595
BsgI GTGCAG 1 cut(s) 744
Bsh1236I CGCG 1 cut(s) 449
BshFI GGCC 5 cut(s) 297, 540, 594, 711, 766
BsiHKCI CYCGRG 1 cut(s) 1195
BsiSI CCGG 5 cut(s) 215, 300, 423, 737, 1151
BsiWI CGTACG 1 cut(s) 897
BslFI GGGAC 4 cut(s) 64, 652, 1403, 1482
BslI CCNNNNNNNGG 5 cut(s) 522, 707, 727, 774, 872
BsmAI GTCTC 3 cut(s) 507, 638, 910
BsmFI GGGAC 4 cut(s) 64, 652, 1403, 1482
BsmI GAATGC 2 cut(s) 324, 1126
BsnI GGCC 5 cut(s) 297, 540, 594, 711, 766
Bso31I GGTCTC 1 cut(s) 507
BsoBI CYCGRG 1 cut(s) 1195
Bsp120I GGGCCC 2 cut(s) 295, 764
Bsp1286I GDGCHC 6 cut(s) 105, 299, 493, 768, 906, 1449
Bsp143I GATC 8 cut(s) 130, 581, 832, 934, 959, 1157, 1209, 1494
Bsp19I CCATGG 1 cut(s) 97
BspACI CCGC 4 cut(s) 202, 449, 870, 957
BspANI GGCC 5 cut(s) 297, 540, 594, 711, 766
BspCNI CTCAG 5 cut(s) 505, 634, 695, 1093, 1474
BspFNI CGCG 1 cut(s) 449
BspHI TCATGA 1 cut(s) 415
BspMAI CTGCAG 1 cut(s) 613
BspOI GCTAGC 2 cut(s) 477, 1115
BspPI GGATC 5 cut(s) 589, 929, 942, 967, 1489
BspTI CTTAAG 1 cut(s) 478
BspTNI GGTCTC 1 cut(s) 507
BsrFI RCCGGY 1 cut(s) 422
BsrI ACTGG 2 cut(s) 516, 1101
BssAI RCCGGY 1 cut(s) 422
BssECI CCNNGG 5 cut(s) 63, 97, 298, 541, 1405
BssMI GATC 8 cut(s) 130, 581, 832, 934, 959, 1157, 1209, 1494
BssT1I CCWWGG 3 cut(s) 63, 97, 541
Bst2UI CCWGG 4 cut(s) 775, 1139, 1365, 1407
Bst4CI ACNGT 3 cut(s) 706, 760, 781
Bst6I CTCTTC 1 cut(s) 38
BstAFI CTTAAG 1 cut(s) 478
BstAPI GCANNNNNTGC 1 cut(s) 1121
BstC8I GCNNGC 6 cut(s) 206, 382, 475, 1113, 1389, 1521
BstDEI CTNAG 6 cut(s) 492, 642, 682, 1001, 1101, 1482
BstDSI CCRYGG 1 cut(s) 97
BstFNI CGCG 1 cut(s) 449
BstHHI GCGC 2 cut(s) 449, 1387
BstKTI GATC 8 cut(s) 133, 584, 835, 937, 962, 1160, 1212, 1497
BstMAI GTCTC 3 cut(s) 507, 638, 910
BstMBI GATC 8 cut(s) 130, 581, 832, 934, 959, 1157, 1209, 1494
BstMWI GCNNNNNNNGC 7 cut(s) 269, 474, 605, 717, 1097, 1121, 1247
BstNI CCWGG 4 cut(s) 775, 1139, 1365, 1407
BstSCI CCNGG 6 cut(s) 213, 298, 773, 1137, 1363, 1405
BstSFI CTRYAG 1 cut(s) 609
BstSLI GKGCMC 3 cut(s) 299, 768, 1449
BstUI CGCG 1 cut(s) 449
BstV1I GCAGC 6 cut(s) 521, 595, 623, 966, 1228, 1292
BstX2I RGATCY 2 cut(s) 934, 959
BstXI CCANNNNNNTGG 1 cut(s) 463
BstYI RGATCY 2 cut(s) 934, 959
BsuRI GGCC 5 cut(s) 297, 540, 594, 711, 766
BtgI CCRYGG 1 cut(s) 97
BtsI GCAGTG 2 cut(s) 375, 595
BtsIMutI CAGTG 4 cut(s) 375, 523, 595, 1094
Cac8I GCNNGC 6 cut(s) 206, 382, 475, 1113, 1389, 1521
CciI TCATGA 1 cut(s) 415
CfoI GCGC 2 cut(s) 449, 1387
Cfr10I RCCGGY 1 cut(s) 422
Cfr13I GGNCC 7 cut(s) 78, 217, 295, 296, 593, 764, 765
CsiI ACCWGGT 1 cut(s) 1137
Csp6I GTAC 2 cut(s) 859, 898
CspCI CAANNNNNGTGG 2 cut(s) 1057, 1092
CviAII CATG 5 cut(s) 21, 98, 320, 416, 797
CviQI GTAC 2 cut(s) 859, 898
DdeI CTNAG 6 cut(s) 492, 642, 682, 1001, 1101, 1482
DpnI GATC 8 cut(s) 132, 583, 834, 936, 961, 1159, 1211, 1496
DpnII GATC 8 cut(s) 130, 581, 832, 934, 959, 1157, 1209, 1494
DraIII CACNNNGTG 1 cut(s) 518
EaeI YGGCCR 1 cut(s) 538
Eam1104I CTCTTC 1 cut(s) 38
EarI CTCTTC 1 cut(s) 38
Eco130I CCWWGG 3 cut(s) 63, 97, 541
Eco24I GRGCYC 5 cut(s) 105, 299, 493, 768, 906
Eco31I GGTCTC 1 cut(s) 507
Eco47I GGWCC 2 cut(s) 78, 217
Eco57I CTGAAG 2 cut(s) 875, 1493
Eco88I CYCGRG 1 cut(s) 1195
EcoO109I RGGNCCY 3 cut(s) 78, 295, 296
EcoRII CCWGG 4 cut(s) 773, 1137, 1363, 1405
EcoT14I CCWWGG 3 cut(s) 63, 97, 541
EcoT22I ATGCAT 2 cut(s) 1126, 1354
EcoT38I GRGCYC 5 cut(s) 105, 299, 493, 768, 906
ErhI CCWWGG 3 cut(s) 63, 97, 541
FaeI CATG 5 cut(s) 24, 101, 323, 419, 800
FalI AAGNNNNNCTT 4 cut(s) 351, 383, 461, 493
FaqI GGGAC 4 cut(s) 64, 652, 1403, 1482
FatI CATG 5 cut(s) 20, 97, 319, 415, 796
FauI CCCGC 1 cut(s) 442
FbaI TGATCA 2 cut(s) 832, 1209
FblI GTMKAC 1 cut(s) 1026
Fnu4HI GCNGC 7 cut(s) 6, 510, 609, 612, 955, 1242, 1281
FriOI GRGCYC 5 cut(s) 105, 299, 493, 768, 906
Fsp4HI GCNGC 7 cut(s) 6, 510, 609, 612, 955, 1242, 1281
FspBI CTAG 5 cut(s) 140, 474, 1112, 1295, 1346
GlaI GCGC 2 cut(s) 448, 1386
GluI GCNGC 7 cut(s) 6, 510, 609, 612, 955, 1242, 1281
GsaI CCCAGC 1 cut(s) 599
GsuI CTGGAG 1 cut(s) 950
HaeIII GGCC 5 cut(s) 297, 540, 594, 711, 766
HapII CCGG 5 cut(s) 215, 300, 423, 737, 1151
HhaI GCGC 2 cut(s) 449, 1387
Hin1II CATG 5 cut(s) 24, 101, 323, 419, 800
Hin6I GCGC 2 cut(s) 447, 1385
HinP1I GCGC 2 cut(s) 447, 1385
HincII GTYRAC 1 cut(s) 1332
HindII GTYRAC 1 cut(s) 1332
HindIII AAGCTT 1 cut(s) 365
HinfI GANTC 4 cut(s) 733, 997, 1291, 1360
HpaII CCGG 5 cut(s) 215, 300, 423, 737, 1151
HphI GGTGA 7 cut(s) 6, 250, 442, 706, 742, 763, 1369
Hpy166II GTNNAC 3 cut(s) 241, 1027, 1332
Hpy188I TCNGA 4 cut(s) 130, 787, 1372, 1483
Hpy188III TCNNGA 7 cut(s) 168, 194, 416, 803, 994, 1060, 1498
Hpy8I GTNNAC 3 cut(s) 241, 1027, 1332
HpyAV CCTTC 6 cut(s) 200, 949, 1189, 1432, 1468, 1531
HpyCH4III ACNGT 3 cut(s) 706, 760, 781
HpyCH4V TGCA 7 cut(s) 8, 227, 611, 725, 1124, 1352, 1391
HpyF10VI GCNNNNNNNGC 7 cut(s) 269, 474, 605, 717, 1097, 1121, 1247
HpyF3I CTNAG 6 cut(s) 492, 642, 682, 1001, 1101, 1482
Hsp92II CATG 5 cut(s) 24, 101, 323, 419, 800
HspAI GCGC 2 cut(s) 447, 1385
KflI GGGWCCC 1 cut(s) 78
Ksp22I TGATCA 2 cut(s) 832, 1209
Kzo9I GATC 8 cut(s) 130, 581, 832, 934, 959, 1157, 1209, 1494
LmnI GCTCC 4 cut(s) 444, 452, 659, 1546
Lsp1109I GCAGC 6 cut(s) 521, 595, 623, 966, 1228, 1292
LweI GCATC 7 cut(s) 238, 409, 702, 778, 796, 1100, 1339
MabI ACCWGGT 1 cut(s) 1137
MaeI CTAG 5 cut(s) 140, 474, 1112, 1295, 1346
MaeIII GTNAC 4 cut(s) 302, 315, 739, 1357
MalI GATC 8 cut(s) 132, 583, 834, 936, 961, 1159, 1211, 1496
MboI GATC 8 cut(s) 130, 581, 832, 934, 959, 1157, 1209, 1494
MboII GAAGA 4 cut(s) 25, 47, 881, 934
MfeI CAATTG 1 cut(s) 1392
MflI RGATCY 2 cut(s) 934, 959
MhlI GDGCHC 6 cut(s) 105, 299, 493, 768, 906, 1449
MlsI TGGCCA 1 cut(s) 540
MluCI AATT 9 cut(s) 600, 616, 884, 1069, 1205, 1217, 1325, 1392, 1410
MluNI TGGCCA 1 cut(s) 540
MlyI GAGTC 2 cut(s) 991, 1354
MmeI TCCRAC 2 cut(s) 160, 503
Mox20I TGGCCA 1 cut(s) 540
Mph1103I ATGCAT 2 cut(s) 1126, 1354
MroXI GAANNNNTTC 1 cut(s) 989
MscI TGGCCA 1 cut(s) 540
MseI TTAA 3 cut(s) 222, 369, 479
MslI CAYNNNNRTG 2 cut(s) 19, 236
Msp20I TGGCCA 1 cut(s) 540
MspA1I CMGCKG 2 cut(s) 608, 957
MspCI CTTAAG 1 cut(s) 478
MspI CCGG 5 cut(s) 215, 300, 423, 737, 1151
MspR9I CCNGG 6 cut(s) 215, 300, 775, 1139, 1365, 1407
MunI CAATTG 1 cut(s) 1392
Mva1269I GAATGC 2 cut(s) 324, 1126
MvaI CCWGG 4 cut(s) 775, 1139, 1365, 1407
MvnI CGCG 1 cut(s) 449
MwoI GCNNNNNNNGC 7 cut(s) 269, 474, 605, 717, 1097, 1121, 1247
NciI CCSGG 2 cut(s) 215, 300
NcoI CCATGG 1 cut(s) 97
NdeII GATC 8 cut(s) 130, 581, 832, 934, 959, 1157, 1209, 1494
NheI GCTAGC 2 cut(s) 473, 1111
NlaIII CATG 5 cut(s) 24, 101, 323, 419, 800
NmuCI GTSAC 1 cut(s) 1357
NsiI ATGCAT 2 cut(s) 1126, 1354
PagI TCATGA 1 cut(s) 415
PctI GAATGC 2 cut(s) 324, 1126
PdmI GAANNNNTTC 1 cut(s) 989
PfeI GAWTC 2 cut(s) 733, 1291
Pfl23II CGTACG 1 cut(s) 897
PflMI CCANNNNNTGG 3 cut(s) 522, 707, 872
PkrI GCNGC 7 cut(s) 7, 511, 610, 613, 956, 1243, 1282
PleI GAGTC 2 cut(s) 991, 1354
PpsI GAGTC 2 cut(s) 991, 1354
PpuMI RGGWCCY 1 cut(s) 78
Psp5II RGGWCCY 1 cut(s) 78
Psp6I CCWGG 4 cut(s) 773, 1137, 1363, 1405
PspFI CCCAGC 1 cut(s) 595
PspGI CCWGG 4 cut(s) 773, 1137, 1363, 1405
PspLI CGTACG 1 cut(s) 897
PspOMI GGGCCC 2 cut(s) 295, 764
PspPI GGNCC 7 cut(s) 78, 217, 295, 296, 593, 764, 765
PspPPI RGGWCCY 1 cut(s) 78
PsrI GAACNNNNNNTAC 2 cut(s) 1137, 1169
PstI CTGCAG 1 cut(s) 613
PsuI RGATCY 2 cut(s) 934, 959
PvuII CAGCTG 1 cut(s) 608
RsaI GTAC 2 cut(s) 860, 899
RsaNI GTAC 2 cut(s) 859, 898
RseI CAYNNNNRTG 2 cut(s) 19, 236
SaqAI TTAA 3 cut(s) 222, 369, 479
SatI GCNGC 7 cut(s) 6, 510, 609, 612, 955, 1242, 1281
Sau3AI GATC 8 cut(s) 130, 581, 832, 934, 959, 1157, 1209, 1494
Sau96I GGNCC 7 cut(s) 78, 217, 295, 296, 593, 764, 765
SchI GAGTC 2 cut(s) 991, 1354
ScrFI CCNGG 6 cut(s) 215, 300, 775, 1139, 1365, 1407
SduI GDGCHC 6 cut(s) 105, 299, 493, 768, 906, 1449
SexAI ACCWGGT 1 cut(s) 1137
SfaNI GCATC 7 cut(s) 238, 409, 702, 778, 796, 1100, 1339
SfcI CTRYAG 1 cut(s) 609
SinI GGWCC 2 cut(s) 78, 217
SmiMI CAYNNNNRTG 2 cut(s) 19, 236
SmlI CTYRAG 3 cut(s) 338, 478, 1187
SmoI CTYRAG 3 cut(s) 338, 478, 1187
Sse9I AATT 9 cut(s) 600, 616, 884, 1069, 1205, 1217, 1325, 1392, 1410
SsiI CCGC 4 cut(s) 202, 449, 870, 957
SspMI CTAG 5 cut(s) 140, 474, 1112, 1295, 1346
StyD4I CCNGG 6 cut(s) 213, 298, 773, 1137, 1363, 1405
StyI CCWWGG 3 cut(s) 63, 97, 541
TaaI ACNGT 3 cut(s) 706, 760, 781
TaqI TCGA 1 cut(s) 1156
TasI AATT 9 cut(s) 600, 616, 884, 1069, 1205, 1217, 1325, 1392, 1410
TatI WGTACW 1 cut(s) 858
TfiI GAWTC 2 cut(s) 733, 1291
Tru1I TTAA 3 cut(s) 222, 369, 479
Tru9I TTAA 3 cut(s) 222, 369, 479
TscAI CASTG 4 cut(s) 382, 523, 595, 1101
TseFI GTSAC 1 cut(s) 1357
TseI GCWGC 7 cut(s) 5, 509, 608, 611, 954, 1241, 1280
Tsp45I GTSAC 1 cut(s) 1357
TspDTI ATGAA 2 cut(s) 60, 785
TspRI CASTG 4 cut(s) 382, 523, 595, 1101
Van91I CCANNNNNTGG 3 cut(s) 522, 707, 872
Vha464I CTTAAG 1 cut(s) 478
VpaK11BI GGWCC 2 cut(s) 78, 217
XapI RAATTY 2 cut(s) 616, 1217
XcmI CCANNNNNNNNNTGG 1 cut(s) 1253
XmiI GTMKAC 1 cut(s) 1026
XmnI GAANNNNTTC 1 cut(s) 989
XspI CTAG 5 cut(s) 140, 474, 1112, 1295, 1346
Zsp2I ATGCAT 2 cut(s) 1126, 1354
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.