Rmu_sc0002064.1_g000004

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002064.1
OG0000427
Physical Location & Seq
Reverse (-)
26650 .. 28023
1374 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002064.1_g000004.1.cds

Sequence Viewer

Length: 963 bp
atgcttattacctttctcttcacattgtcatctcttccaggcacattcgcagcccgcagattctcactaaaagaagccaccatagacgatctccagctcactttcaagcacaaccaactcacctcgaggcaacttgttcaatattaccttgcccaaatccgaaaactgaacccggttctcaaaggagtcctagaagtcaatccagatgcgcttcaccttgccgacaaggctgactacgagcgcaggaccaaggcaccagtgtcactactctctaagttgcatggcattcctattctggtcaaggacaacattgcaaccaaggataagatgaacaccacagccggctcttttgcactgcttggatctgtcgtgcctcgtgatgctggcgtggtggagaagttgaggaaagctggggctatcattttgggaaaggctagcttgagcgagtggtcacattttaggtctactaaaactccctctggttggagcgctagaggcggttatggcttgaacccttacacattctcagacccttgcggctcaagcagcggttcagcaatatcagtggcagcaaatctggcagcggtgtcattaggtactgagacaagtggttcaatcttatgtccatcaagtttgaactcagttgtgggcctcaaaccaacagtcggtcttactagtcgagcaggggttgtcccaatttctctaagacaggatactgttgggccaatttgtaggacagtagcagatgctgcttatgttcttgatgccatagcagggatcgaccacaatgacattgcaacaattgagacatcaaagtacattcccaaaggtggttatgcacagtttctcaggcgcgacggacttagtggaaaaagaatagggatattgggagtcttccatgactttggcaatgacacttccttggctcaaacttttgagaaacatctcaacactctgaggtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

320

Amino Acids

34.21

Weight (kDa)

9.59

Isoelectric Point (pI)

31.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 124
AccB1I GGYRCC 1 cut(s) 253
AccI GTMKAC 1 cut(s) 464
AccII CGCG 1 cut(s) 855
AciI CCGC 5 cut(s) 55, 498, 537, 549, 584
AclWI GGATC 2 cut(s) 370, 785
AfaI GTAC 2 cut(s) 598, 818
AfeI AGCGCT 1 cut(s) 490
AfiI CCNNNNNNNGG 4 cut(s) 483, 665, 774, 830
AgsI TTSAA 5 cut(s) 106, 140, 511, 615, 637
AhlI ACTAGT 1 cut(s) 674
AjnI CCWGG 1 cut(s) 37
AluBI AGCT 3 cut(s) 97, 410, 438
AluI AGCT 3 cut(s) 97, 410, 438
Alw26I GTCTC 2 cut(s) 596, 800
AlwI GGATC 2 cut(s) 370, 785
AlwNI CAGNNNCTG 1 cut(s) 749
Ama87I CYCGRG 1 cut(s) 124
Aor51HI AGCGCT 1 cut(s) 490
AoxI GGCC 2 cut(s) 649, 722
ApeKI GCWGC 5 cut(s) 50, 546, 569, 581, 749
ArsI GACNNNNNNTTYG 2 cut(s) 648, 680
AspLEI GCGC 4 cut(s) 211, 243, 491, 855
AspS9I GGNCC 3 cut(s) 246, 649, 722
AsuC2I CCSGG 1 cut(s) 173
AsuHPI GGTGA 2 cut(s) 112, 206
AsuNHI GCTAGC 1 cut(s) 434
AvaI CYCGRG 1 cut(s) 124
AvaII GGWCC 1 cut(s) 246
BanI GGYRCC 1 cut(s) 253
BauI CACGAG 1 cut(s) 375
BbsI GAAGAC 1 cut(s) 886
BbvI GCAGC 5 cut(s) 62, 558, 581, 593, 736
BccI CCATC 1 cut(s) 634
BciT130I CCWGG 1 cut(s) 39
BciVI GTATCC 1 cut(s) 706
BcnI CCSGG 1 cut(s) 173
BcoDI GTCTC 2 cut(s) 596, 800
BcuI ACTAGT 1 cut(s) 674
BfaI CTAG 4 cut(s) 191, 435, 492, 675
BfoI RGCGCY 1 cut(s) 492
BfuI GTATCC 1 cut(s) 706
BglI GCCNNNNNGGC 1 cut(s) 227
BisI GCNGC 6 cut(s) 51, 538, 547, 570, 582, 750
BlsI GCNGC 6 cut(s) 52, 539, 548, 571, 583, 751
Bme1390I CCNGG 2 cut(s) 39, 173
Bme18I GGWCC 1 cut(s) 246
BmeT110I CYCGRG 1 cut(s) 124
BmgT120I GGNCC 3 cut(s) 246, 649, 722
BmiI GGNNCC 1 cut(s) 255
BmrFI CCNGG 2 cut(s) 39, 173
BmsI GCATC 4 cut(s) 196, 370, 736, 754
BmtI GCTAGC 1 cut(s) 438
BpiI GAAGAC 1 cut(s) 886
BpmI CTGGAG 1 cut(s) 77
BpuEI CTTGAG 2 cut(s) 460, 526
BpuMI CCSGG 1 cut(s) 173
BsaJI CCNNGG 3 cut(s) 249, 318, 921
BsaXI ACNNNNNCTCC 2 cut(s) 457, 487
Bsc4I CCNNNNNNNGG 4 cut(s) 483, 665, 774, 830
Bse118I RCCGGY 1 cut(s) 341
Bse1I ACTGG 1 cut(s) 257
Bse3DI GCAATG 3 cut(s) 309, 792, 916
BseBI CCWGG 1 cut(s) 39
BseDI CCNNGG 3 cut(s) 249, 318, 921
BseLI CCNNNNNNNGG 4 cut(s) 483, 665, 774, 830
BseMI GCAATG 3 cut(s) 309, 792, 916
BseMII CTCAG 5 cut(s) 540, 591, 654, 862, 947
BseNI ACTGG 1 cut(s) 257
BseXI GCAGC 5 cut(s) 62, 558, 581, 593, 736
BseYI CCCAGC 1 cut(s) 410
Bsh1236I CGCG 1 cut(s) 855
BshFI GGCC 2 cut(s) 651, 724
BshNI GGYRCC 1 cut(s) 253
BsiHKCI CYCGRG 1 cut(s) 124
BsiSI CCGG 2 cut(s) 173, 342
BslFI GGGAC 1 cut(s) 677
BslI CCNNNNNNNGG 4 cut(s) 483, 665, 774, 830
BsmAI GTCTC 2 cut(s) 596, 800
BsmFI GGGAC 1 cut(s) 677
BsmI GAATGC 1 cut(s) 285
BsnI GGCC 2 cut(s) 651, 724
BsoBI CYCGRG 1 cut(s) 124
Bsp143I GATC 3 cut(s) 88, 362, 777
BspACI CCGC 5 cut(s) 55, 498, 537, 549, 584
BspANI GGCC 2 cut(s) 651, 724
BspCNI CTCAG 5 cut(s) 539, 592, 653, 861, 948
BspFNI CGCG 1 cut(s) 855
BspLI GGNNCC 1 cut(s) 255
BspOI GCTAGC 1 cut(s) 438
BspPI GGATC 2 cut(s) 370, 785
BspT107I GGYRCC 1 cut(s) 253
BsrDI GCAATG 3 cut(s) 309, 792, 916
BsrFI RCCGGY 1 cut(s) 341
BsrI ACTGG 1 cut(s) 257
BssAI RCCGGY 1 cut(s) 341
BssECI CCNNGG 3 cut(s) 249, 318, 921
BssMI GATC 3 cut(s) 88, 362, 777
BssSI CACGAG 1 cut(s) 375
BssT1I CCWWGG 3 cut(s) 249, 318, 921
Bst2BI CACGAG 1 cut(s) 375
Bst2UI CCWGG 1 cut(s) 39
Bst4CI ACNGT 4 cut(s) 664, 718, 739, 843
Bst6I CTCTTC 2 cut(s) 23, 39
BstAPI GCANNNNNTGC 1 cut(s) 749
BstC8I GCNNGC 4 cut(s) 55, 343, 385, 436
BstDEI CTNAG 8 cut(s) 273, 526, 600, 640, 704, 848, 863, 956
BstFNI CGCG 1 cut(s) 855
BstH2I RGCGCY 1 cut(s) 492
BstHHI GCGC 4 cut(s) 211, 243, 491, 855
BstKTI GATC 3 cut(s) 91, 365, 780
BstMAI GTCTC 2 cut(s) 596, 800
BstMBI GATC 3 cut(s) 88, 362, 777
BstMWI GCNNNNNNNGC 7 cut(s) 227, 495, 504, 543, 546, 578, 749
BstNI CCWGG 1 cut(s) 39
BstSCI CCNGG 2 cut(s) 37, 171
BstUI CGCG 1 cut(s) 855
BstV1I GCAGC 5 cut(s) 62, 558, 581, 593, 736
BstV2I GAAGAC 1 cut(s) 886
BstX2I RGATCY 1 cut(s) 362
BstXI CCANNNNNNTGG 1 cut(s) 905
BstYI RGATCY 1 cut(s) 362
BsuI GTATCC 1 cut(s) 706
BsuRI GGCC 2 cut(s) 651, 724
BtsI GCAGTG 1 cut(s) 353
BtsIMutI CAGTG 3 cut(s) 264, 353, 570
Cac8I GCNNGC 4 cut(s) 55, 343, 385, 436
CaiI CAGNNNCTG 1 cut(s) 749
CfoI GCGC 4 cut(s) 211, 243, 491, 855
Cfr10I RCCGGY 1 cut(s) 341
Cfr13I GGNCC 3 cut(s) 246, 649, 722
Csp6I GTAC 2 cut(s) 597, 817
CspCI CAANNNNNGTGG 2 cut(s) 546, 581
CviAII CATG 2 cut(s) 281, 899
CviQI GTAC 2 cut(s) 597, 817
DdeI CTNAG 8 cut(s) 273, 526, 600, 640, 704, 848, 863, 956
DpnI GATC 3 cut(s) 90, 364, 779
DpnII GATC 3 cut(s) 88, 362, 777
Eam1104I CTCTTC 2 cut(s) 23, 39
EarI CTCTTC 2 cut(s) 23, 39
Eco130I CCWWGG 3 cut(s) 249, 318, 921
Eco47I GGWCC 1 cut(s) 246
Eco47III AGCGCT 1 cut(s) 490
Eco88I CYCGRG 1 cut(s) 124
EcoRII CCWGG 1 cut(s) 37
EcoT14I CCWWGG 3 cut(s) 249, 318, 921
ErhI CCWWGG 3 cut(s) 249, 318, 921
FaeI CATG 2 cut(s) 284, 902
FaiI YATR 8 cut(s) 83, 282, 504, 622, 756, 770, 837, 900
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FaqI GGGAC 1 cut(s) 677
FatI CATG 2 cut(s) 280, 898
FauI CCCGC 1 cut(s) 62
FblI GTMKAC 1 cut(s) 464
Fnu4HI GCNGC 6 cut(s) 51, 538, 547, 570, 582, 750
Fsp4HI GCNGC 6 cut(s) 51, 538, 547, 570, 582, 750
FspBI CTAG 4 cut(s) 191, 435, 492, 675
GlaI GCGC 4 cut(s) 210, 242, 490, 854
GluI GCNGC 6 cut(s) 51, 538, 547, 570, 582, 750
GsaI CCCAGC 1 cut(s) 414
GsuI CTGGAG 1 cut(s) 77
HaeII RGCGCY 1 cut(s) 492
HaeIII GGCC 2 cut(s) 651, 724
HapII CCGG 2 cut(s) 173, 342
HhaI GCGC 4 cut(s) 211, 243, 491, 855
Hin1II CATG 2 cut(s) 284, 902
Hin6I GCGC 4 cut(s) 209, 241, 489, 853
HinP1I GCGC 4 cut(s) 209, 241, 489, 853
HinfI GANTC 3 cut(s) 60, 186, 891
HpaII CCGG 2 cut(s) 173, 342
HphI GGTGA 2 cut(s) 112, 206
Hpy166II GTNNAC 1 cut(s) 465
Hpy188I TCNGA 3 cut(s) 161, 529, 957
Hpy188III TCNNGA 3 cut(s) 203, 377, 761
Hpy8I GTNNAC 1 cut(s) 465
Hpy99I CGWCG 1 cut(s) 860
HpyCH4III ACNGT 4 cut(s) 664, 718, 739, 843
HpyCH4V TGCA 5 cut(s) 280, 314, 353, 797, 839
HpyF10VI GCNNNNNNNGC 7 cut(s) 227, 495, 504, 543, 546, 578, 749
HpyF3I CTNAG 8 cut(s) 273, 526, 600, 640, 704, 848, 863, 956
Hsp92II CATG 2 cut(s) 284, 902
HspAI GCGC 4 cut(s) 209, 241, 489, 853
KroI GCCGGC 1 cut(s) 341
KroNI GCCGGC 1 cut(s) 343
Kzo9I GATC 3 cut(s) 88, 362, 777
LmnI GCTCC 1 cut(s) 486
Lsp1109I GCAGC 5 cut(s) 62, 558, 581, 593, 736
LweI GCATC 4 cut(s) 196, 370, 736, 754
MaeI CTAG 4 cut(s) 191, 435, 492, 675
MaeIII GTNAC 2 cut(s) 261, 450
MalI GATC 3 cut(s) 90, 364, 779
MboI GATC 3 cut(s) 88, 362, 777
MboII GAAGA 3 cut(s) 10, 26, 886
MfeI CAATTG 1 cut(s) 801
MflI RGATCY 1 cut(s) 362
MluCI AATT 3 cut(s) 696, 726, 801
MlyI GAGTC 2 cut(s) 195, 900
MmeI TCCRAC 1 cut(s) 464
MnlI CCTC 8 cut(s) 120, 133, 384, 396, 487, 488, 662, 951
MroNI GCCGGC 1 cut(s) 341
MspA1I CMGCKG 2 cut(s) 549, 584
MspI CCGG 2 cut(s) 173, 342
MspR9I CCNGG 2 cut(s) 39, 173
MunI CAATTG 1 cut(s) 801
Mva1269I GAATGC 1 cut(s) 285
MvaI CCWGG 1 cut(s) 39
MvnI CGCG 1 cut(s) 855
MwoI GCNNNNNNNGC 7 cut(s) 227, 495, 504, 543, 546, 578, 749
NaeI GCCGGC 1 cut(s) 343
NciI CCSGG 1 cut(s) 173
NdeII GATC 3 cut(s) 88, 362, 777
NgoMIV GCCGGC 1 cut(s) 341
NheI GCTAGC 1 cut(s) 434
NlaIII CATG 2 cut(s) 284, 902
NlaIV GGNNCC 1 cut(s) 255
NmuCI GTSAC 2 cut(s) 261, 450
PaeR7I CTCGAG 1 cut(s) 124
PctI GAATGC 1 cut(s) 285
PdiI GCCGGC 1 cut(s) 343
PfeI GAWTC 1 cut(s) 60
PkrI GCNGC 6 cut(s) 52, 539, 548, 571, 583, 751
PleI GAGTC 2 cut(s) 194, 899
PpsI GAGTC 2 cut(s) 194, 899
Psp6I CCWGG 1 cut(s) 37
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 255
PspPI GGNCC 3 cut(s) 246, 649, 722
PspXI VCTCGAGB 1 cut(s) 124
PstNI CAGNNNCTG 1 cut(s) 749
PsuI RGATCY 1 cut(s) 362
RsaI GTAC 2 cut(s) 598, 818
RsaNI GTAC 2 cut(s) 597, 817
SatI GCNGC 6 cut(s) 51, 538, 547, 570, 582, 750
Sau3AI GATC 3 cut(s) 88, 362, 777
Sau96I GGNCC 3 cut(s) 246, 649, 722
SchI GAGTC 2 cut(s) 195, 900
ScrFI CCNGG 2 cut(s) 39, 173
SfaNI GCATC 4 cut(s) 196, 370, 736, 754
Sfr274I CTCGAG 1 cut(s) 124
SinI GGWCC 1 cut(s) 246
SlaI CTCGAG 1 cut(s) 124
SmlI CTYRAG 3 cut(s) 124, 439, 541
SmoI CTYRAG 3 cut(s) 124, 439, 541
SpeI ACTAGT 1 cut(s) 674
Sse9I AATT 3 cut(s) 696, 726, 801
SsiI CCGC 5 cut(s) 55, 498, 537, 549, 584
SspI AATATT 1 cut(s) 143
SspMI CTAG 4 cut(s) 191, 435, 492, 675
StyD4I CCNGG 2 cut(s) 37, 171
StyI CCWWGG 3 cut(s) 249, 318, 921
TaaI ACNGT 4 cut(s) 664, 718, 739, 843
TaqI TCGA 3 cut(s) 125, 679, 780
TaqII GACCGA 1 cut(s) 656
TasI AATT 3 cut(s) 696, 726, 801
TatI WGTACW 1 cut(s) 816
TauI GCSGC 1 cut(s) 540
TfiI GAWTC 1 cut(s) 60
TscAI CASTG 3 cut(s) 264, 360, 570
TseFI GTSAC 2 cut(s) 261, 450
TseI GCWGC 5 cut(s) 50, 546, 569, 581, 749
Tsp45I GTSAC 2 cut(s) 261, 450
TspDTI ATGAA 1 cut(s) 344
TspGWI ACGGA 1 cut(s) 873
TspRI CASTG 3 cut(s) 264, 360, 570
VpaK11BI GGWCC 1 cut(s) 246
XhoI CTCGAG 1 cut(s) 124
XmiI GTMKAC 1 cut(s) 464
XspI CTAG 4 cut(s) 191, 435, 492, 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.