Rmu_sc0002522.1_g000023

Amidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002522.1
OG0000427
Physical Location & Seq
Reverse (-)
80669 .. 82011
1343 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002522.1_g000023.1.cds

Sequence Viewer

Length: 855 bp
atggtttctgtgacactagggacggagaccgacggctccattatctgtccggctagctttaactcagtaattggcgtcaaaccgactgttggtctcactagtcgagctggagtcatcccaatcagtccaagacaggacacagttgggccgatttgtaggactgtagcggatgctgttcacgtccttgatatcattgttggcattgacaggaatgacaatgcaacaagtgaagcatcaaagtacatcccagtgggaggctatggacagtttctcaagcataatggtctcgaaggaaagagattggggatagtaaatttgttatttgattcaatcaagcacgatgtttttctgaaacaaacttttgagcagcattttaccacactgaggaatcagggggcagttttggttgacaatcttgaaatagcccatattgaagatgttctggattctcattcaagtggagaatttgatgcaatgttagctgaattcgaaatagccttgaatgagtacctaagtgacctagtaaaatctccagtgaagtccttaagggaggtgatagagttcaacaagaatcattcaaaggtggaaaagatcaaggaatatgggcaagatatcttcgaattagctgagaagactaatgggatgggaccgaaagagcaggaggcatttgcttcttctatcctcgcaattggtggttttccaggcattgttgtgctggcaggatatcatgacactaaaaagtatccttacgggatctgctttgggggactcaggggttcagagccaaagctgattgagatcacctatgccttcgagcaagcaactaagataaggagaaaacctcctctagcctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0016787 GO:0016810 GO:0016811
KEGG Pathways
Metabolic & Signaling
ko00330 ko00360 ko00380 ko00627 ko00643 ko01120 map00330 map00360 map00380 map00627 map00643 map01120
Pfam Domains
Protein Families
Amidase

Protein Analysis

284

Amino Acids

30.99

Weight (kDa)

5.65

Isoelectric Point (pI)

35.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000427)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G34880 AT4G34880
fragaria_vesca FvH4_2g01710 FvH4_2g01720 FvH4_2g01722
malus_domestica MD05G1117000.v1.1 MD05G1117200.v1.1 MD10G1120200.v1.1 MD10G1120300.v1.1 MD10G1120400.v1.1
prunus_persica Prupe.8G161600_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161700_v2.0.a1 Prupe.8G161800_v2.0.a1 Prupe.8G161900_v2.0.a1
pyrus_communis pycom05g06760 pycom10g10360 pycom10g10370 pycom17g11700
rosa_chinensis RchiOBHm_Chr6g0244611 RchiOBHm_Chr6g0244621 RchiOBHm_Chr6g0244631 RchiOBHm_Chr6g0244671 RchiOBHm_Chr6g0244701 RchiOBHm_Chr6g0244721 RchiOBHm_Chr6g0244741 RchiOBHm_Chr6g0244751
rosa_laevigata RLG00000015331 RLG00000015332 RLG00000015333 RLG00000015334 RLG00000015336 RLG00000015338 RLG00000015339 RLG00000015340
rosa_multiflora Rmu_co8154324.1_g000001 Rmu_co8259027.1_g000001 Rmu_co8319583.1_g000001 Rmu_sc0002064.1_g000004 Rmu_sc0002522.1_g000021 Rmu_sc0002522.1_g000022 Rmu_sc0002522.1_g000023 Rmu_sc0002522.1_g000026 Rmu_sc0002522.1_g000027 Rmu_sc0006257.1_g000002 Rmu_sc0006635.1_g000001 Rmu_sc0006635.1_g000002 Rmu_sc0007349.1_g000001 Rmu_sc0011292.1_g000009 Rmu_sc0011292.1_g000010 Rmu_sc0039466.1_g000001
rosa_roxburghii Rroxscaffold_7G00215530 Rroxscaffold_7G00215550 Rroxscaffold_7G00215560 Rroxscaffold_7G00215570 Rroxscaffold_7G00215610 Rroxscaffold_7G00215620 Rroxscaffold_7G00215630 Rroxscaffold_7G00215640
rosa_rugosa Rorug05G0509900 Rorug05G0510000 Rorug05G0510000 Rorug05G0510100 Rorug05G0510100 Rorug05G0510200 Rorug05G0510300 Rorug05G0510400
rosa_samantha Rh6BG020500 Rh6BG020600 Rh6BG020700 Rh6BG020900 Rh6BG021000 Rh6BG021100 Rh6BG021200 Rh6CG018800 Rh6CG019000 Rh6CG019100 Rh6CG019200 Rh6CG019300 Rh6CG019400 Rh6CG019600 Rh6CG019800 Rh6DG019900 Rh6DG020100 Rh6DG020200 Rh6DG020400
rosa_wichuraiana Rw0G014670 Rw6G000010 Rw6G000020 Rw6G002100 Rw6G002110 Rw6G002120 Rw6G002140 Rw6G002150 Rw6G002160 Rw6G005110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 167
AclWI GGATC 1 cut(s) 761
AcsI RAATTY 3 cut(s) 313, 464, 485
AcyI GRCGYC 1 cut(s) 75
AfaI GTAC 2 cut(s) 242, 509
AfiI CCNNNNNNNGG 3 cut(s) 89, 254, 384
AflII CTTAAG 1 cut(s) 544
AgsI TTSAA 7 cut(s) 330, 419, 434, 456, 502, 565, 579
AhdI GACNNNNNGTC 1 cut(s) 90
AhlI ACTAGT 1 cut(s) 98
AjiI CACGTC 1 cut(s) 181
AjnI CCWGG 1 cut(s) 700
AluBI AGCT 5 cut(s) 57, 107, 482, 626, 790
AluI AGCT 5 cut(s) 57, 107, 482, 626, 790
Alw26I GTCTC 3 cut(s) 20, 98, 290
AlwI GGATC 1 cut(s) 761
AoxI GGCC 1 cut(s) 146
ApeKI GCWGC 1 cut(s) 367
ApoI RAATTY 3 cut(s) 313, 464, 485
Asp700I GAANNNNTTC 1 cut(s) 438
AspS9I GGNCC 2 cut(s) 146, 647
AsuHPI GGTGA 2 cut(s) 565, 793
AsuII TTCGAA 2 cut(s) 489, 618
AsuNHI GCTAGC 1 cut(s) 53
AvaII GGWCC 1 cut(s) 647
BbsI GAAGAC 1 cut(s) 638
BbvI GCAGC 1 cut(s) 379
BccI CCATC 1 cut(s) 637
BceAI ACGGC 1 cut(s) 49
BciT130I CCWGG 1 cut(s) 702
BciVI GTATCC 1 cut(s) 753
BcoDI GTCTC 3 cut(s) 20, 98, 290
BcuI ACTAGT 1 cut(s) 98
BfaI CTAG 5 cut(s) 17, 54, 99, 521, 848
BfmI CTRYAG 1 cut(s) 162
BfrI CTTAAG 1 cut(s) 544
BfuI GTATCC 1 cut(s) 753
BisI GCNGC 1 cut(s) 368
BlsI GCNGC 1 cut(s) 369
Bme1390I CCNGG 1 cut(s) 702
Bme18I GGWCC 1 cut(s) 647
BmeRI GACNNNNNGTC 1 cut(s) 90
BmgBI CACGTC 1 cut(s) 181
BmgT120I GGNCC 2 cut(s) 146, 647
BmiI GGNNCC 2 cut(s) 37, 648
BmrFI CCNGG 1 cut(s) 702
BmrI ACTGGG 1 cut(s) 242
BmsI GCATC 3 cut(s) 160, 242, 460
BmtI GCTAGC 1 cut(s) 57
BmuI ACTGGG 1 cut(s) 242
BpiI GAAGAC 1 cut(s) 638
BplI GAGNNNNNCTC 1 cut(s) 826
BpmI CTGGAG 2 cut(s) 129, 516
Bpu14I TTCGAA 2 cut(s) 489, 618
BpuEI CTTGAG 1 cut(s) 257
BsaBI GATNNNNATC 1 cut(s) 797
BsaHI GRCGYC 1 cut(s) 75
BsaI GGTCTC 3 cut(s) 20, 98, 290
Bsc4I CCNNNNNNNGG 3 cut(s) 89, 254, 384
Bse1I ACTGG 2 cut(s) 248, 533
Bse3DI GCAATG 1 cut(s) 480
Bse8I GATNNNNATC 1 cut(s) 797
BseBI CCWGG 1 cut(s) 702
BseGI GGATG 4 cut(s) 114, 175, 243, 648
BseJI GATNNNNATC 1 cut(s) 797
BseLI CCNNNNNNNGG 3 cut(s) 89, 254, 384
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 4 cut(s) 78, 374, 618, 784
BseNI ACTGG 2 cut(s) 248, 533
BseRI GAGGAG 1 cut(s) 834
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 1 cut(s) 148
BsiSI CCGG 1 cut(s) 50
BslFI GGGAC 3 cut(s) 34, 660, 780
BslI CCNNNNNNNGG 3 cut(s) 89, 254, 384
BsmAI GTCTC 3 cut(s) 20, 98, 290
BsmFI GGGAC 3 cut(s) 34, 660, 780
BsnI GGCC 1 cut(s) 148
Bso31I GGTCTC 3 cut(s) 20, 98, 290
Bsp119I TTCGAA 2 cut(s) 489, 618
Bsp143I GATC 3 cut(s) 591, 753, 798
BspACI CCGC 1 cut(s) 167
BspANI GGCC 1 cut(s) 148
BspCNI CTCAG 4 cut(s) 77, 375, 619, 783
BspHI TCATGA 1 cut(s) 727
BspLI GGNNCC 2 cut(s) 37, 648
BspOI GCTAGC 1 cut(s) 57
BspPI GGATC 1 cut(s) 761
BspT104I TTCGAA 2 cut(s) 489, 618
BspTI CTTAAG 1 cut(s) 544
BspTNI GGTCTC 3 cut(s) 20, 98, 290
BsrDI GCAATG 1 cut(s) 480
BsrI ACTGG 2 cut(s) 248, 533
BssMI GATC 3 cut(s) 591, 753, 798
BssNI GRCGYC 1 cut(s) 75
Bst2UI CCWGG 1 cut(s) 702
Bst4CI ACNGT 4 cut(s) 88, 142, 163, 267
BstACI GRCGYC 1 cut(s) 75
BstAFI CTTAAG 1 cut(s) 544
BstBI TTCGAA 2 cut(s) 489, 618
BstC8I GCNNGC 3 cut(s) 55, 717, 819
BstDEI CTNAG 6 cut(s) 64, 383, 512, 627, 770, 825
BstF5I GGATG 4 cut(s) 114, 175, 243, 648
BstKTI GATC 3 cut(s) 594, 756, 801
BstMAI GTCTC 3 cut(s) 20, 98, 290
BstMBI GATC 3 cut(s) 591, 753, 798
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 1 cut(s) 702
BstSCI CCNGG 1 cut(s) 700
BstSFI CTRYAG 1 cut(s) 162
BstV1I GCAGC 1 cut(s) 379
BstV2I GAAGAC 1 cut(s) 638
BstX2I RGATCY 1 cut(s) 753
BstYI RGATCY 1 cut(s) 753
BsuI GTATCC 1 cut(s) 753
BsuRI GGCC 1 cut(s) 148
BtrI CACGTC 1 cut(s) 181
BtsCI GGATG 4 cut(s) 114, 175, 243, 648
BtsIMutI CAGTG 3 cut(s) 255, 380, 540
Cac8I GCNNGC 3 cut(s) 55, 717, 819
CciI TCATGA 1 cut(s) 727
Cfr13I GGNCC 2 cut(s) 146, 647
CseI GACGC 1 cut(s) 64
Csp6I GTAC 2 cut(s) 241, 508
CviAII CATG 1 cut(s) 728
CviQI GTAC 2 cut(s) 241, 508
DdeI CTNAG 6 cut(s) 64, 383, 512, 627, 770, 825
DpnI GATC 3 cut(s) 593, 755, 800
DpnII GATC 3 cut(s) 591, 753, 798
DriI GACNNNNNGTC 1 cut(s) 90
Eam1105I GACNNNNNGTC 1 cut(s) 90
Eco31I GGTCTC 3 cut(s) 20, 98, 290
Eco32I GATATC 3 cut(s) 190, 613, 725
Eco47I GGWCC 1 cut(s) 647
EcoRI GAATTC 1 cut(s) 485
EcoRII CCWGG 1 cut(s) 700
EcoRV GATATC 3 cut(s) 190, 613, 725
FaeI CATG 1 cut(s) 731
FaiI YATR 6 cut(s) 261, 279, 429, 603, 729, 807
FaqI GGGAC 3 cut(s) 34, 660, 780
FatI CATG 1 cut(s) 727
Fnu4HI GCNGC 1 cut(s) 368
FokI GGATG 4 cut(s) 101, 182, 230, 655
Fsp4HI GCNGC 1 cut(s) 368
FspBI CTAG 5 cut(s) 17, 54, 99, 521, 848
GluI GCNGC 1 cut(s) 368
GsuI CTGGAG 2 cut(s) 129, 516
HaeIII GGCC 1 cut(s) 148
HapII CCGG 1 cut(s) 50
HgaI GACGC 1 cut(s) 64
Hin1I GRCGYC 1 cut(s) 75
Hin1II CATG 1 cut(s) 731
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HinfI GANTC 6 cut(s) 111, 326, 388, 446, 571, 768
HpaII CCGG 1 cut(s) 50
HphI GGTGA 2 cut(s) 565, 793
Hpy166II GTNNAC 2 cut(s) 178, 409
Hpy188I TCNGA 2 cut(s) 351, 781
Hpy188III TCNNGA 4 cut(s) 287, 416, 443, 728
Hpy8I GTNNAC 2 cut(s) 178, 409
Hpy99I CGWCG 1 cut(s) 35
HpyAV CCTTC 2 cut(s) 284, 820
HpyCH4III ACNGT 4 cut(s) 88, 142, 163, 267
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 2 cut(s) 221, 473
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 6 cut(s) 64, 383, 512, 627, 770, 825
HpySE526I ACGT 1 cut(s) 180
Hsp92I GRCGYC 1 cut(s) 75
Hsp92II CATG 1 cut(s) 731
Kzo9I GATC 3 cut(s) 591, 753, 798
LmnI GCTCC 1 cut(s) 41
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 3 cut(s) 160, 242, 460
MaeI CTAG 5 cut(s) 17, 54, 99, 521, 848
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 2 cut(s) 10, 515
MalI GATC 3 cut(s) 593, 755, 800
MboI GATC 3 cut(s) 591, 753, 798
MboII GAAGA 4 cut(s) 446, 607, 643, 666
MfeI CAATTG 1 cut(s) 687
MflI RGATCY 1 cut(s) 753
MluCI AATT 6 cut(s) 69, 313, 464, 485, 620, 687
MlyI GAGTC 2 cut(s) 120, 762
MnlI CCTC 7 cut(s) 248, 378, 544, 655, 692, 852, 855
MroXI GAANNNNTTC 1 cut(s) 438
MseI TTAA 2 cut(s) 60, 545
MslI CAYNNNNRTG 3 cut(s) 248, 456, 710
MspCI CTTAAG 1 cut(s) 544
MspI CCGG 1 cut(s) 50
MspR9I CCNGG 1 cut(s) 702
MunI CAATTG 1 cut(s) 687
MvaI CCWGG 1 cut(s) 702
MwoI GCNNNNNNNGC 1 cut(s) 479
NdeII GATC 3 cut(s) 591, 753, 798
NheI GCTAGC 1 cut(s) 53
NlaIII CATG 1 cut(s) 731
NlaIV GGNNCC 2 cut(s) 37, 648
NmuCI GTSAC 2 cut(s) 10, 515
NspV TTCGAA 2 cut(s) 489, 618
PagI TCATGA 1 cut(s) 727
PdmI GAANNNNTTC 1 cut(s) 438
PfeI GAWTC 4 cut(s) 326, 388, 446, 571
PkrI GCNGC 1 cut(s) 369
PleI GAGTC 2 cut(s) 119, 762
PpsI GAGTC 2 cut(s) 119, 762
Psp6I CCWGG 1 cut(s) 700
PspGI CCWGG 1 cut(s) 700
PspN4I GGNNCC 2 cut(s) 37, 648
PspPI GGNCC 2 cut(s) 146, 647
PsuI RGATCY 1 cut(s) 753
RsaI GTAC 2 cut(s) 242, 509
RsaNI GTAC 2 cut(s) 241, 508
RseI CAYNNNNRTG 3 cut(s) 248, 456, 710
SaqAI TTAA 2 cut(s) 60, 545
SatI GCNGC 1 cut(s) 368
Sau3AI GATC 3 cut(s) 591, 753, 798
Sau96I GGNCC 2 cut(s) 146, 647
SchI GAGTC 2 cut(s) 120, 762
ScrFI CCNGG 1 cut(s) 702
SfaNI GCATC 3 cut(s) 160, 242, 460
SfcI CTRYAG 1 cut(s) 162
SfuI TTCGAA 2 cut(s) 489, 618
SinI GGWCC 1 cut(s) 647
SmiMI CAYNNNNRTG 3 cut(s) 248, 456, 710
SmlI CTYRAG 2 cut(s) 272, 544
SmoI CTYRAG 2 cut(s) 272, 544
SpeI ACTAGT 1 cut(s) 98
Sse9I AATT 6 cut(s) 69, 313, 464, 485, 620, 687
SsiI CCGC 1 cut(s) 167
SspMI CTAG 5 cut(s) 17, 54, 99, 521, 848
StyD4I CCNGG 1 cut(s) 700
TaaI ACNGT 4 cut(s) 88, 142, 163, 267
TaiI ACGT 1 cut(s) 183
TaqI TCGA 5 cut(s) 103, 288, 489, 618, 813
TaqII GACCGA 2 cut(s) 44, 664
TasI AATT 6 cut(s) 69, 313, 464, 485, 620, 687
TatI WGTACW 1 cut(s) 240
TfiI GAWTC 4 cut(s) 326, 388, 446, 571
Tru1I TTAA 2 cut(s) 60, 545
Tru9I TTAA 2 cut(s) 60, 545
TscAI CASTG 3 cut(s) 255, 387, 540
TseFI GTSAC 2 cut(s) 10, 515
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 2 cut(s) 10, 515
TspGWI ACGGA 1 cut(s) 38
TspRI CASTG 3 cut(s) 255, 387, 540
Vha464I CTTAAG 1 cut(s) 544
VpaK11BI GGWCC 1 cut(s) 647
XapI RAATTY 3 cut(s) 313, 464, 485
XmnI GAANNNNTTC 1 cut(s) 438
XspI CTAG 5 cut(s) 17, 54, 99, 521, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.